KR20130070107A - A method for preparing functional green tea extract comprising abundant functional ingredient and the cosmetic composition comprising the same having potent anti-wrinkle activity - Google Patents
A method for preparing functional green tea extract comprising abundant functional ingredient and the cosmetic composition comprising the same having potent anti-wrinkle activity Download PDFInfo
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Abstract
Description
The present invention relates to an extraction method for extracting a functional green tea fermented product containing a large amount of functional ingredients and a cosmetic composition for improving wrinkles.
M. B. Kim, Tea of Korea, Tam Gu Dang, 1983, 322.
Sung, KC, Characteristics and Analysis on the Refined Oil Component of Green-Tea, J. of Korean Oil Chmist's Soc . 2005, 22 (3), 241-249.
3.Han, JS, Shin, DH, Yun, SE and Kim, MS Antimicrobial Effects on Listeria Monocytogenes by Some Edible Plants Extracts Korean J. Food . Sci . Technol . 1994, 26; 545.
Ji, BJ, Chow, WH, Hscing, AW, Mclaughlin, JK, Dai, G., Gao, YT, Blot, WJ and Fraumeni, JF Green-Tea Comsumption and the Risk of Pancreatic and Colorectal Cancers, Int. J. Cancer . 1997, 70, 255.
5.Shan, S. G, Katitar, SK, Agarwal, R. and Mukhtar, H. Enhancement of Antioxidant and Phase II Enzymes by Oral Feeding of Green-Tea PolyPhenols in Drinking Water to SKH-1 Hairless Mice; Possible Role in Cancer Chemoprevention, Cancer Res . 1992, 52; 4050.
6. Ji BT, Chow WH, Hsing AW, McLaughlin JK, Dai Q, Gao YT, et al. Green tea comsumption and the risk of pancreatic and colorectal cancers. Int . J. Cancer . 1997, 70, 255-8.
7.J. W. Yang, Antioxidant Effects and Extract Yield to Catechins from Green-Tea by Various Solvents, Department of Food Sci., Of Korea Univ. Graduate School, 2004, pp 1-2.
8. Salah N, Miller NJ, Paganga G, Tijburg L, Bo [wel] GP, Rice-Evans C. Polyphenolic flavanols as scavengers of aqueous phase radicals and as chain breaking antioxidants. Arch . Biochem. Biophys . 1995, 322, 339-346.
9.Kelly C, Hunter K, Crosibie L, Gordon MJ, Dutta-Roy AK, Modulation of human platelet function by food flavonoid. Biochem . Soc . Trans 1996, 24, 197S.
10. Hara Y. Influence of tea catechins on the digestive tract. J. cell Biochem. Suppl . 1997, 27, 52-58.
11.Suzuki H, Ishigaki A, Hara Y. Long-term effect of a trace amount of tea catechins with perilla oil on the plasma lipids in mice. Int . J. Vitam . Nutr . Res . 1998, 68, 272-274.
12.Shibata K, Moriyama M, Fukushima T, Miyazaki M, Une H. Green tea consumption and chronic atrophic gastritis: a cross-sectional study in green tea protection village. J. Epidemiol . 2000, 10, 310-316.
13. Hodgson JM, Puddey IB, Burke V, Jordan N. .Effects on blood pressure of drinking green and black tea. J. Hypertens . 1999, 17, 457-463.
14. Blois MS. Antioxidant determination by the use of a stable free radical. Nature . 1958; 26:
15. Bury M, Gerhards J, Erni W, Stamm A. Application of a new method based on conductivity measurements to determine the creaming stability of o / w emulsions. Int . J. Pharm . 1995; 124 (2): 183)
16. Amerine MA, Ough CS, 1980. Method for analysis of musts and wine, Wiley & Sons, Chichester. P.176-180
17. Byeonghwa, Instrumental Evaluation of Skin Effect, Korean Society of Skin Barriers, Vol. 8, No. 1
Historically, green tea has been cultivated for the first time in China and India, and has since spread to Southeast Asia such as Java, Ceylon, Myanmar, Thailand, and East Asia in Korea and Japan, mainly in Asia below 35 ° C. Has come. 1) Green tea has the scientific name Camellia sinensis L., and it is a perennial seed plant belonging to the tea tree family. It has a natural fragrance, natural color, natural taste, antibacterial action, antioxidant activity, anticancer activity, and anticancer activity. allergic reactions, blood pressure lowering action, anti-diabetic activity, diuretic and detoxification is known that the human body works and pharmacological properties, including one of the oldest symbols of food 2-5), the most widely consumed in the world. Green tea goes through a long history been used in various diseases and drug cultivation, manufacturing technology advances and 6) There is increasing interest in green tea as it is reported that green tea maintains health and suppresses the occurrence of diseases such as cancer. Green tea is composed of various ingredients and contains caffeine, tannin, chlorophyll, vitamins A, B 1 , B 2 , C, E and amino acids, proteins, fats, fiber, and inorganic components Ca, Mg, Cu, Mo , Sb, Ti and other components are variously contained. Among these components, caffeine and tannin are composed of polyphenol, an aromatic extract, which consists of cellulose, lignin, and catechin having several or more phenolic hydroxyl groups in the molecule. Among the polyphenol components contained in green tea, catechins are representative bioactive substances. 7) in particular including (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicatechin-3-gallate (ECG), (-)-epigallocatechin-3-gallate (EGCG), and the like. Most studies on polyphenols have been conducted 8-10 ) . Green tea catechins are known to possess various physiological activity such as anti-inflammatory, anti-allergic and anti-cancer as natural antioxidants 12-13).
Green tea catechin is a natural antioxidant known to have various physiological activities such as anti-inflammatory, anti-allergic and anti-cancer. Recently, tea has been used as an additive, such as food, medicine, health food, cosmetics by simply extracting functional ingredients from tea leaves as well as drinking beverages. Tea extracts for this purpose have been used for extracting dried tea by processing the green leaves of tea by thickening or steaming food process, but using hot water extract. There has been a problem that the cost increase factor such as
Therefore, in this study, we tried to prepare a new behavioral tea extract by enzymatic treatment in order to obtain high quality extract with improved extraction efficiency of green tea. The extract was confirmed that the wrinkle improvement effect is excellent and completed the present invention.
In order to achieve the above object, the present invention is the first step of adding water to the dry green tea and adding a hydrolase thereto; A second step of obtaining a fermentation product by performing an enzyme reaction while culturing and stirring the solution; A third step of heating and extracting the fermented product; It provides a manufacturing method for producing a fermented green tea extract containing a large amount of the functional component comprising the step of filtering and extracting the extract and separating the supernatant.
In the first step of the preparation method, water is about 1 to 100 times (w / w), preferably about 5 to 50 times (w / w), more preferably about 10 to 30 times (w) the weight of green tea. / w) weight of water, and the hydrolase is preferably a hydrolase that is mainly composed of cellulase, β-glucosidase, xylanase, and more. Preferably, Viscozyme L (enzyme activity of 100FBG / g, FBG = fungal beta-glucanse units Novo Nordisk Co., Denmark Product No. KTN02199) was prepared in an amount from about 0.001 to 50% (v / v), preferably about 0.01 to 5% (v / v), more preferably about 0.1 to 2% (v) of the solution volume. v / v) volume of enzyme is added.
In the second step of the preparation method, the solution is preferably 30 minutes to 1 week, more preferably 1 hour to 56 hours, even more preferably 12 hours to 48 hours, most preferably 18 hours to For 24 hours, incubated at 0 to 100 ° C., preferably at about 10 to 60 ° C., more preferably at about 30 to 50 ° C., with stirring being stirred at 100 rpm to 500 rpm, preferably 150 rpm to 250 rpm Characterized by carrying out an enzymatic reaction.
In the third step of the production method, the fermented product is extracted for about 10 minutes to 24 hours, preferably 30 hours to 2 hours at a temperature of about 10 to 100 ℃, preferably 60 to 90 ℃ .
The fermented green tea extract prepared by the above method provides a high extraction yield and a high content of total phenolic extract, which is a functional ingredient, and at the same time shows a strong anti-wrinkle effect in the mouse experimental model with strong antioxidant effect and skin aging. It may be usefully used as a cosmetic composition for skin aging and wrinkle improvement.
In another aspect, the present invention provides a cosmetic composition for improving wrinkles containing the fermented green tea extract containing a large amount of the functional ingredient obtained by the production method and the production method as an active ingredient.
In addition, the cosmetic composition includes a lotion, skin, lotion, nutrition lotion, nutrition cream, massage cream, essence, the formulation of the pack.
In addition, green tea catechins have been used for herbal medicine and food for a long time, the fermented green tea extract of the present invention extracted from them also has no problems such as toxicity and side effects, and it is proved to be a non-irritating sample in skin patch test, so it is safe even for long-term use. Can be used.
The external skin pharmaceutical composition containing the extract of the present invention may be prepared as a pharmaceutical composition in the form of an external skin preparation of cream, gel, patch, spray, ointment, warning, lotion, linen, pasta or cataplasma. However, it is not limited thereto.
Preferred dosages of the extracts of the present invention vary depending on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, for the preferred effect, the extract of the present invention is preferably administered at 0.0001 to 100 mg / kg, preferably 0.001 to 10 mg / kg per day. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.
The extract of the present invention can be used variously in cosmetics and cleansers having a whitening effect.
Examples of products to which the present composition can be added include cosmetics such as lotion, skin, lotion, nutrition lotion, nutritional cream, massage cream, essence, pack, cleansing, cleanser, soap, have.
The cosmetic composition of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high molecular weight peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.
The water-soluble vitamin is not particularly limited as long as it can be compounded in cosmetics. Preferably, vitamin B, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, And their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbate-2-phosphate etc.) can also be added to water-soluble vitamins . The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.
Usable vitamins include vitamins such as vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) , Derivatives thereof (such as palmitic acid ascorbin, stearic acid ascorbic acid, dipalmitic acid ascorbin, dl-alpha tocopherol acetic acid, dl-alpha tocopherol nicotinic acid vitamin E, dl-pantothenyl alcohol, D-pantothenyl alcohol, Ether, etc.) are also included in the usable vitamins used in the present invention. Usability Vitamins can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method.
The polymeric peptide may be any compound as long as it can be compounded in cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.
The polymeric polysaccharide may be any compound as long as it can be incorporated in cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.
Sphingo lipids may be any as long as they can be incorporated into cosmetics, and preferable examples thereof include ceramides, phytosphingosine and sphingoglycolipids. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.
The seaweed extract may be any of those which can be compounded in cosmetics. Preferably, the seaweed extract is selected from the group consisting of algae extract, red pepper extract, green algae extract and the like. Also, the algae extract may be colored guanine, arginic acid, Potassium alginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained from seaweed by a conventional method.
The cosmetic of the present invention may be blended with other essential ingredients, if necessary, in combination with the essential ingredients.
Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.
Examples of the oil retaining component include ester-based oil retaining, hydrocarbon-based oil retaining, silicone-based oil retaining, fluoric oil retaining, animal retention and plant retention.
Examples of ester-based fats include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearyl isostearate, Butyl isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isosilyl myristate, isostearic acid isostearyl, isostearyl palmitate, octyldodecyl myristate, Trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic acid pentaerythritol tetra (2-ethylhexanoate) , Decyl caprylate, decyl laurate, hexyl laurate, myristate decyl, myristyl myristate, myristine monoethyl stearate, stearyl stearate, decyl oleate, ricinoleic acid tri , Isostearyl stearate, isostearyl stearate, isodecyl stearate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, isopropyl stearate, isopropyl stearate, isopropyl stearate, -Hexyl stearate, stearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol, Propyleneglycol propionate, propyleneglycol propionate, dicaproic acid neopentyl glycol, dioctanoic acid neopentyl glycol, tricarboxylic acid glyceryl, triunsaturated glyceryl, triisopalmitic acid glyceryl, triisostearic acid glyceryl, neopentanoic acid octyldodecyl Octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, Octyldecyl lactate, octyldecyl lactate, octyldecyl lactate, polyglycerin oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisobutyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, But are not limited to, ethyl, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, But are not limited to, dioctyl sebacate, stearic acid cholesteryl, isostearic acid cholesteryl, hydroxystearic acid cholesteryl, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid pitostearyl, Stearoyl hydroxystearic acid isostearyl, 12-stearoyl stearyl hydroxystearate, 12-stearo And monohydroxystearic acid and esters such as sostearyl.
Examples of the hydrocarbon hydrocarbon-based fats include hydrocarbon fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline.
Examples of silicone based oils include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstarchoxysiloxane copolymer, alkyl Modified silicone oils, and amino-modified silicone oils.
Examples of the fluorine-based oil include perfluoropolyether and the like.
Examples of animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, , Corn oil, palm oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, coltsfoot colostrum, marker daisy nut oil, mead home oil, egg oil, , Canned wax, carnauba wax, liquid lanolin, hardened castor oil, and the like.
Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers.
Examples of the water-soluble low-molecular moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like.
Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol and cholesterol ester.
Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. .
Examples of the oil-soluble polymer include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.
Examples of the emollients include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid and lanolin fatty acid cholesteryl ester.
Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.
Examples of the nonionic surfactant include self emulsifying monostearate glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester, POE (Polyoxyethylene / polyoxypropylene) copolymer, POE.POP alkyl ether, polyether-modified silicone, polyether-modified silicone, polyoxyethylene-polyoxypropylene (POE) Alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.
Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylarylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, Alkylsulfosuccinic acid salts, acylated hydrolyzed collagen peptide salts, and perfluoroalkyl phosphoric acid esters, and the like can be mentioned. have.
As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.
Examples of the amphoteric surfactant include carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amide amine type Amphoteric surfactants and the like.
Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.
As the organic powder, metallic soap such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.
Examples of ultraviolet absorbers include paraaminobenzoic acid, ethyl parnamobenzoate, amyl paranobenzoate, octyl paranobenzoate, ethyleneglycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate , Octyl methoxycinnamate, dioctyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl paratumoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Carninoic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino- p- (carbo-2'-ethylhexyl-1'- , 3,5-triazine, 2- (2- And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.
As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.
Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.
Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.
Examples of the alcohol include higher alcohols such as cetyl alcohol.
In addition, any of the above components may be blended within the range not to impair the objects and effects of the present invention, but it is preferably 0.01 to 5% by weight based on the total weight, Preferably 0.01 to 3% by weight.
The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.
The ingredients contained in the cosmetic composition of the present invention may contain, in addition to the above-mentioned extracts, the components conventionally used in cosmetic compositions, such as stabilizers, solubilizers, vitamins, pigments and fragrances, And a carrier.
The cosmetic composition of the present invention can be prepared into any formulation conventionally produced in the art, and examples thereof include emulsions, creams, lotions, packs, foundations, lotions, essences, and hair cosmetics.
Specifically, the cosmetic composition of the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, Packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.
When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .
In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.
In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.
When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.
When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.
The production method of the present invention can provide a functional green tea fermentation product containing a large amount of functional components exhibiting various physiological activities, and the extract prepared by the production method shows a strong anti-wrinkle effect and a strong wrinkle improvement effect in a skin aging-induced mouse experimental model. Bar, it can be usefully used as a cosmetic composition for skin aging and wrinkle improvement.
1 is a view showing a manufacturing process for producing a fermented green tea extract (FT) of the present invention;
Figure 2 is a diagram showing the effect of inhibiting skin aging after 5 weeks of hairless mouse applied according to the enzyme-treated green tea extract concentration.
Hereinafter, the present invention will be described in detail with reference to the following Examples and Experimental Examples.
However, the following examples and experimental examples are illustrative of the present invention, and the content of the present invention is not limited by the following examples and experimental examples.
Example 1. Preparation of Fermented Green Tea Extract (see FIG. 1)
1-1. Experimental material
The green tea used in this experiment was dried leaf green tea (purchased from the medicinal market in June-August from Gyeongnam Ha-dong) and the enzyme used for green tea extraction was commercially used as an enzyme used for hydrolysis. Viscozyme L (Novo Nordisk Co., Denmark Product No. KTN02199), the main ingredient of which is cellulase, β-glucosidase, and xylanase, was used.
1-2.Enzyme-treated Green Tea Extract Preparation
After adding 200 mL of distilled water to 10 g of green tea, the enzyme concentration (0, 0.5, 1, 2% by volume) and the enzyme reaction time (0, 6, 12, 18, 24 hr) were varied and stirred at 40 rpm at 200 rpm. It was. Enzyme-treated green tea was heated for 1 hour at 80 ° C for enzyme inactivation and extraction improvement. After filtration through a 20 mesh sieve, centrifugation was performed at 1000g for 30 minutes (Beckman Coulter, Optima TM L-100XP ultracentrifuge) and dried. 1 ± 0.5g of fermented green tea extract was prepared, which was used as a sample for physicochemical quality analysis.
1-3.Preparation of Sample (Enzyme-treated Green Tea Extract Concentrate)
Enzyme treatment conditions set as the optimum enzyme treatment conditions 1:20 ratio of green tea and distilled water,
Example 2. Preparation of Cosmetic (Essence) Compositions with Different Concentrate Concentrations
The fermented green tea extract obtained in the above Example was used to prepare cosmetic compositions for various uses as follows (see Table 1).
(1) The temperature of the water phase is warmed up to 80 ° C and completely mixed with a pedal mixer.
(2) Warm up to 75 ~ 80 ℃ in a separate heating container of oil contents and cool completely.
(3) The contents of the oil phase portion are added to the water phase portion, and the homomixer is mixed and mixed at a stirring speed of 3200 rpm for 5 minutes.
(4) After cooling to 45 ° C., the additive part is added to the mixed content of the water phase part and the oil phase part, followed by mixing for 2 minutes with a stirring speed of 2500 rpm and a 12 rpm pedal mixer and degassing.
Experimental Example 1. Determination of soluble solids content and Electron donating ability Measure
1-1. Soluble solid content measurement
In order to measure the soluble solids content of the enzyme-treated green tea extract, 20 mL of the extract was taken in a volumetric formula, evaporated to dryness at 105 ° C., and the weight thereof was measured. Soluble solid content (%) was calculated | required as
1-1-1. Determination of Total Phenolic Extract Content
The total phenolic extract content of each extract was colorimetrically determined according to the Folin-Denis method (Amerine MA, Ough CS, 1980. Method for analysis of musts and wine, Wiley & Sons, Chichester. P. 176-180).
In other words, add 5 mL of 5 mL Folin-Ciocalteu reagent diluted 100-fold, and mix. After 3 minutes, shake 5 mL of 10% Na 2 CO 3, shake at room temperature for 1 hour, and absorbance at 700 nm (Optizen 2120UV, Mecasys). Co., Ltd. Daejeon Korea). The control was treated in the same manner by adding distilled water instead of the sample solution. At this time, gallic acid (Sigma chemical Co., USA, G7384) was prepared at the concentration of 5 ~ 50㎍ / mL to prepare a calibration curve.
1-1-2. Total Catechin Quantification
Catechin analysis of green tea extract was quantified by HPLC. Each extract was concentrated and dissolved in 80% methanol and 1 mL was passed through a 0.45 μm membrane filter (Waters Co., Milford, Mass., USA) to use 10 uL for HPLC analysis. At this time, the instrument used as analytical conditions is HPLC (Waters 2796, Waters Co., USA) system, column is YMC-Pack Pro C18 (5㎛, 4.6 ID × 250mm, YMC Inc., USA), mobile phase is solvent A ( 4.5% formalic acid in H2O) and solvent B (90% CH3CN containing 10% solvent A) were used. Detector was performed under UV 280nm, flow rate 1.0 mL / min, sensitivity 0.01 AUFS. In addition, to identify the catechins of the extract, epigallocatechin (EGC, Sigma chemical Co., USA, E3768), epigallocatechin gallate (EGCG, Sigma chemical Co., USA E4143), epicatechin (EC Sigma chemical Co., USA, E1753) and epicatechin gallate (ECG Sigma chemical Co., USA, E3893) were each dissolved in methanol to prepare a mg / mL solution. Total catechin content was calculated by adding the four catechins.
1-2. Electron donating ability Measure
An experiment as follows by using a method described in the literature in order to determine the sample electron donating activity of getting in Example was carried out (Blois MS Antioxidant determination by the use of a stable free radical Nature 1958; 26....: pp1199-120).
The electron donating abilities (EDA) were measured by modifying Blois' method 13 ) as follows. After dissolving 12 mg of DPPH reagent (1,1-diphenyl-2-picrylhydrazyl Sigma chemical Co., USA) in 100 mL of absolute ethanol, add 100 mL of distilled water and test the absorbance of DPPH solution at 517 nm using a 50% ethanol solution as a blank test. After adjusting to 1.0, take 5 mL of this solution, mix with 0.5 mL of sample solution, and leave it for 30 seconds at room temperature. Measure the absorbance at 517 nm (Optizen 2120UV, Mecasys Co., Ltd Daejeon Korea) The difference was expressed as a percentage (%) to give an electron donating ability (see Equation 1).
The experimental results are as follows.
1-2-1. Physicochemical Quality Characteristics According to Enzyme Treatment Concentration
The extraction yield, total phenolic extract content, and electron donating ability of the extract extracted by stirring at 200 rpm for 120 minutes by treating different enzyme concentrations at 0, 0.5, 1.0, 2.0% are shown in Table 1. In other words, the extraction yield, total phenolic extract content, and electron donating ability were increased with increasing enzyme treatment concentration, especially after 1% enzyme treatment concentration. In particular, the total phenolic extract content was high when the enzyme concentration was 1%, and there was no significant difference from the addition of 2% enzyme concentration. (See Table 2)
1-2-2. Chemical Quality Characteristics According to Enzyme Treatment Time
The physicochemical quality characteristics of green tea extracted by fixing the enzyme treatment concentration to 1% and varying the enzyme treatment time to 0, 6, 12, 18 and 24 hours are shown in Table 2. In other words, the extraction yield increased as the enzyme treatment time increased, and the increase was not significant after 18 hours. In addition, the total phenolic extract content also increased with increasing enzyme treatment time, and the increase was not significant. The electron donating ability, which indicates antioxidant activity, also increased as the enzyme treatment time increased, but no significant difference was found between 18 and 24 hours treatment (see Table 3).
1-2-3. Changes in Functional Components According to Enzyme Treatment Conditions
The analysis results of four types of catechins, namely, glass type epicatechin (EC), epigallocatechin (EGC), ester type EGCG, and ECG, which are the main components of green tea catechin, are shown in Tables 3 and 4. All the test plots contained EGC, EC, ECG and EGCG in order, and the EGC content was found to be almost the majority. The content of EGC, EC, ECG, and EGCG increased with increasing enzyme concentration and decreased after 1%. The enzyme treatment time increased with increasing time, but decreased after 18 hours. In addition, the total catechin content was found to be high at the
Experimental Example 2. Evaluation of wrinkle improvement effect using experimental animals
In order to confirm the wrinkle improvement effect of the extract sample obtained in the above example, the experiment was performed by applying the method disclosed in the literature (small pathogenesis, device evaluation of the skin effect, Korean Journal of Skin Barriers Journal, Vol. 8 No. 1)
2-1. Laboratory animals Experimental group
Experimental animals were fed 6-7 weeks old (22-28 g) female hairless mouse (Orient Bio, Tokyo, Japan) and used for 1 week in the nursery. Feed and water were supplied freely for the entire period. The nursery was kept at a temperature of 22 ± 1 o C, a relative humidity of 50 ± 5% and a lighting cycle of 12 hours. The experimental group was divided into four groups according to the cosmetic composition test examples 1, 2, 3, 4, a total of 20 animals in each group was used for the experiment.
2-2. Causes skin aging and sample application
Ultraviolet radiation dose was measured by UV-radiometer (UVB LP9021, Italy), and the mouse was placed in an ultraviolet irradiation cage, and the amount of light at 60 mJ / cm 2 was equally increased to 1 MED at 1 week intervals at 1 MED. 3 times a week, every other day for 5 weeks. After inducing photoaging for 5 weeks, cosmetics (essences) prepared by mixing the green tea extract concentrates at concentrations of 0, 0.1, and 0.5%, respectively, were applied as 200 μL once a day for 5 weeks as a sample.
2-3. Wrinkle measurement according to sample application by concentration
Five weeks after the start of the experiment, each group was lightly anesthetized with ether, and in order to objectively evaluate the degree of wrinkles, it was replicated using silicone polymer (SILFLO impression material, Flexico, England) on the back of the animal. ), The shape of the wrinkles was observed with a skin visometer (SV600, CK electronic GmbH, Germany).
As a result of the experiment, as shown in FIG. 2, the wrinkles of the dorsal template (reflica) of the experimental animal were examined by applying a sample through a skin visometer. In the essence application group containing 0.5% of green tea concentrate and the essence application group containing 1% of green tea concentrate, the lines of wrinkles were markedly thin and the depth was shallow. These results are expected that the application of green tea extract-containing cosmetics (essences) is effective in the improvement and prevention of wrinkles that are a typical sign of skin aging.
In addition, the results, the
The green tea extract thus extracted was concentrated so that the solid content was 30%, and the content of the concentrate was applied to the skin of the mouse, and the wrinkle improvement effect was measured after applying 0.5 ~ 1% concentrate in the cosmetic (essence) composition. It has been shown to have an anti wrinkle effect.
Hereinafter, formulations of cream, massage cream, lotion, skin lotion, essence, pack, and cleansing foam are exemplified as the formulation examples of the present invention, but the formulations including the cosmetic composition of the present invention are not limited thereto.
Formulation Example 1. Cream composition
The oil phase and water phase are heated to 75 ° C and cooled to room temperature.
Formulation Example 2. Massage Cream Composition
The oil phase and water phase are mixed by heating at 75 DEG C and then cooled to room temperature.
Formulation Example 3. lotion composition
The oil phase and water phase are mixed and emulsified by heating at 75 ° C and then cooled to room temperature.
Formulation Example 4. Skin lotion composition
The water phase and the ethanol phase are respectively prepared and mixed and then filtered.
Formulation Example 5. Essence composition
The water phase and the ethanol phase are respectively prepared and mixed and then filtered.
Formulation Example 6. Pack composition
The water phase and the ethanol phase are dispersively dissolved and mixed, and then cooled to room temperature.
Formulation Example 7. Cleansing Foam Composition
The water phase and the oil phase are dispersed and dissolved, mixed and sieved, and then cooled to room temperature.
Claims (9)
In the first step of the manufacturing method, the water is characterized in that to add water of 1 to 100 times (w / w) weight of the green tea weight.
In the first step of the production method, the hydrolase is a cellulase (cellulase), β-glucosidase (glucosidase), xylalanase (xylanase) is a manufacturing method, characterized in that the hydrolysis enzyme as a main component.
In the first step of the production method, the enzyme is characterized in that the addition of 0.001 to 50% (v / v) volume of the enzyme of the solution volume.
In the second step of the manufacturing method, the solution is characterized in that the culture for 30 minutes to one week, 0 to 100 ℃.
In the second step of the production method, the agitation is characterized in that for performing the enzyme reaction while stirring at 100rpm to 500rpm.
In the third step of the manufacturing method, the fermented product is characterized in that extracted for 10 minutes to 24 hours at a temperature of 10 to 100 ℃.
The cosmetic composition is a cosmetic composition, characterized in that the formulation of the lotion, skin, lotion, nutrition lotion, nutrition cream, massage cream, essence, pack.
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Cited By (2)
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CN109694886A (en) * | 2017-10-19 | 2019-04-30 | 安琪酵母股份有限公司 | A kind of green tea fermentation filtrate and its preparation method and application |
KR20190054542A (en) * | 2017-11-14 | 2019-05-22 | (주)아모레퍼시픽 | Method for manufacturing green tea extract, and green tea extract therefrom |
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Cited By (3)
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CN109694886A (en) * | 2017-10-19 | 2019-04-30 | 安琪酵母股份有限公司 | A kind of green tea fermentation filtrate and its preparation method and application |
CN109694886B (en) * | 2017-10-19 | 2022-07-01 | 安琪纽特股份有限公司 | Green tea fermentation filtrate and preparation method and application thereof |
KR20190054542A (en) * | 2017-11-14 | 2019-05-22 | (주)아모레퍼시픽 | Method for manufacturing green tea extract, and green tea extract therefrom |
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