KR20130038737A

KR20130038737A - Sorghum extracts to inhibit absorption of cholesterol and lipids in small intestine

Info

Publication number: KR20130038737A
Application number: KR1020110103261A
Authority: KR
Inventors: 서명철; 우관식; 강종래; 이재생; 송석보; 오병근; 고지연; 곽도연; 남민희; 노상규
Original assignee: 대한민국(관리부서:농촌진흥청장); 창원대학교 산학협력단
Priority date: 2011-10-10
Filing date: 2011-10-10
Publication date: 2013-04-18

Abstract

PURPOSE: A Sorghum extract which suppresses lipid absorption in small intestine is provided to treat hyperlipidemia, to suppress lipid absorption rate, and to ensure various applicabilities. CONSTITUTION: A pharmaceutical composition which treats hyperlipidemia and suppresses lipid absorption in small intestine contains a Sorghum extract as an active ingredient. The extract is prepared by pulverizing Sorghum, extracting with an organic solvent, and concentrating and freeze-drying the extract. A health functional food which suppresses cholesterol and lipid absorption contains the extract as an active ingredient. The health food is used in the form of a powder, a granule, a tablet, a capsule, a syrup, or a drink.

Description

Sorghum extracts to inhibit absorption of cholesterol and lipids in small intestine}

The present invention relates to the inhibitory effect of small intestine fat absorption rate of red sorghum extract, and more specifically, the radioactive isotope is measured using an intestinal mesenteric lymph duct cannulated rat model in vivo. It relates to the inhibitory effect of red sorghum extract not measured by measuring the absorption of cholesterol (14C-cholesterol) contained.

Sorghum, Sorghum bicolor L. Moench) is a perennial herb (Kim et al., 2006) with a monocotyledonous plant, which is native to tropical Africa and is cultivated in arid regions, and grain sorghum, sorgo, and small sorghum Broom-corn is cultivated and is a major food resource in Asia, Africa and Central America. Sorghum is an important grain, followed by rice, barley, wheat and corn, and contains a large amount of active ingredients such as dietary fiber and phenolic compounds. Most of phenolic compounds are known as flavonoids, and recent studies on physiological functionalities of sorghum Is being reported. Although sorghum has high value for the development of new processed foods such as baby food, alcohol, rice cake, bread, malt, and porridge, research on this has been insufficient.

Cardiovascular disease is the leading cause of mortality in human disease and is closely related to blood levels of blood fats, particularly cholesterol and triglycerides. Hypercholesterolemia is one of the major causes of cardiovascular disease. Flavonoids, which are found in abundance in nature such as cereals, fruits, and green tea, are known to protect the cardiovascular system and lower related diseases.

Blood circulation system fat is divided into dietary fats (dietary lipids) and endogenous lipids (endogenous lipids) according to the type of fat flowing into the blood circulation system. In other words, blood fat is largely a dietary fat that enters through the small intestine organs, and some fat that remains in the blood circulation system after metabolism and distribution as endogenous fat is produced in liver tissue and interacts with non-hepatic tissues. And the sum of the remaining fat secreted through the bile secretion pathway, a dynamic interplay product. Fat components of the blood circulation are greatly influenced by the rate of absorption, secretion, or metabolism of these major tissues. The small intestine is the influx of blood circulation fat and the secretion of bile is the only pathway of release of this blood circulation fat. In this regard, research on the effect of inhibiting dietary fat inflow through the small intestine through the use of sorghum extracts rich in active ingredients such as physiologically active phenolic compounds is more important than when frozen.

The hypocholesterolemic effect of sorghum has experimental results suggesting that, in terms of absorption of the small intestine, it may induce inhibition of fat absorption metabolism and finally reduce the frequency of cardiovascular disease. Cho et al (2003) reported that sorghum hexane extracts increased bile acid excretion through feces, and Al-Mamary et al (2001) also reported that sorghum tannins inhibit the activity of pancreatic lipase.

In relation to the explanation of this suppression phenomenon, sorghum's hypercholesterolemic activity is weakly convincing by the excellent antioxidant ability of sorghum, which is explained in many studies above. Inhibition of fat in the small intestine, in particular, inhibition of cholesterol absorption, suppression of cholesterol intestine absorption by sorghum tannin, or inhibition of ileal reuptake of bile acids should not have a greater effect. However, there are no studies on the effects of sorghum extract on small intestine fat absorption at home and abroad.

In relation to studies on the possibility of sorghum extracts inhibiting intestinal lipolysis, Al-Mamary et al. (2001) reported that sorghum tannins inhibit lipase activity in the duodenum in proportion. And the fact that the enzymatic activity of pancreatic lipase is reduced by the tannin component. Lindahl et al. (1997) also found that the activity of pancreatic phospholipase A ₂ (PLA ₂ ) is inhibited by tannins such as hesperetin or myricetin, which are representative flavonoids. Reported. Optimal enzymatic activity of these enzymes in the digestion of fat is essential for the absorption of fat, so if the digestion and degradation of fat by these enzymes is delayed or reduced, the small intestinal The formation of micelles in the lumen is reduced, which inhibits intestinal cell wall entry and subsequent cellular esterification and re-synthesis of dietary fats, including cholesterol, which will ultimately inhibit uptake. . In light of these considerations, sorghum extracts are likely to inhibit the intestinal fat absorption.

Therefore, the present invention, using the sorghum extract to suppress the influx of dietary fat in the small intestine organs associated with the treatment and alleviation of cholesterol and triacylglycerolemia (hypertriacylglycerolemia) has been tried in various fields. Therefore, the present invention is to determine how to suppress the absorption of the small intestine of the main fat when the red-based sorghum-based solvent extraction to feed at the dietary amount level.

The present invention used an intestinal mesenteric lymph duct-cannulated rat model to observe whether sorghum extract affects small intestine fat absorption. This model has long been applied to the development of new drugs using gastrointestinal physiology as a means of studying the interaction between exogenous or dietary factors and lipophilic compounds in the intestinal digestion and absorption of fat. This method involves the absorption of physiologically related absorption physiology of the stomach, bile duct, pancreas, duodenum, small intestine, and small intestine lymphoid system in a live animal model. (Gastrointestinal physiology) with almost no damage to the gastrointestinal tract and gastric tubing connected to the duodenum through the stomach by micro-surgery under anesthesia under anesthesia. Biomeasures directly the intestine's fat absorption rate by connecting the suture of the lymph duct cannula and putting the dietary approach and the corresponding absorption rate as experimental variables for 8 hours after a certain recovery time. It is a model.

This model was used to obtain a US patent in 2004 (United States Patent No. US 6,727,277; Date of Patent: April 27, 2004. Compounds affecting cholesterol absorption; Duy H. Hua, Sung I. Koo & Sang K. Noh ).

A representative example of this type of study in the form of extracts is the amount of extract equivalent to one to two cups of green tea that humans consume, reducing the intestinal absorption of cholesterol in a dose-dependent manner. There is a study result (FIG. 1).

Based on the theory known to you and your own research experience, set the following goals. "Sorghum extract inhibits the intestinal uptake of ¹⁴ C-cholesterol and triglycerides in the intestinal mesenteric lymph duct-cannulated rat model." This has not been attempted at home or abroad under these experimental conditions, and it is the first animal bioassay to check the effect of sorghum extract on the intestinal absorption rate of dietary fat.

This experimental data is a quantitative data explaining that sorghum extract is a natural extract effective in suppressing the intestinal fat absorption associated with hyperlipidemia treatment and alleviation. In addition, the sorghum extract is a physiological functional extract effective in treating hyperlipidemia and having various applications.

1 is a diagram illustrating the inhibition of ¹⁴ C-cholesterol ( ¹⁴ C-CH) small intestinal absorption by green tea extract as an example of the description of the means. GT0, 0.0 mg GT extract; GT1, 42.9 mg GT; GT2, 120.5 mg GT (20).
2 is a graph showing the effect of sorghum extract for 8 hours on the small intestine absorption rate of ¹⁴ C-cholesterol.
3 is a graph showing the effect of sorghum extract on small intestine absorption rate of α-tocophenol (αTP) for 8 hours.
4 is a graph showing the effect of sorghum extract on small intestine absorption of retinol (ROH) for 8 hours.
5 is a graph showing the effect of sorghum extract on the absorption rate of phospholipid (PL) for 8 hours.

Referring to the present invention in more detail as follows.

Experimental Example 1 Extraction of Sorghum Extract

The sorghum extract used for animal experiments was grown and harvested by the National Crop Research Division, 4,600 rpm using a Pin-type Mill (DK201, Sejung Tech, Daegu, Korea). The mixture was shaken three times for 24 hours at 50 ° C. with 80% methanol (Wise Cube WIS-RL010, Daihan Scientific Co., Ltd., Seoul, Korea), and then filtered and concentrated under reduced pressure (Eyela N-1000, Tokyo, Japan) and lyophilization (FDT-8612, OPERON, Kimpo, Korea) to obtain 40g was used as a sample while stored in a -20 ° C freezer.

Experimental Example 2: animals, dietary and breeding conditions

The diet used for animal experiments was obtained from the powdered Dyets Inc. (Bethlehem, PA, USA) AIN-93G diet recommended by the National Institute of Nutrition (AIN) through the Central Laboratory Animal. The mineral content of this diet was generally adjusted to egg whites, and fats were raised on a standard diet using soybean oil. Dietary composition is shown in Table 1.

Component Content (g / kg) Component Content (g / kg) Egg whites 200.0 Mineral mix 35.0 Corn starch 528.5 Vitamin mix 10.0 Crystalline glucose 100.0 Biotin 4.0 fibrin 50.0 Choline Hydrogen Tartrate 2.5 Soybean oil (0.02% tert-butylhydroauinone) 70.0

The animal used to develop the rat absorption experiment model was male Sprague-Dawley rat (Harlan Sprague Dawley, Inc.), and the initial average weight was 250.6 g. , Standard humidity and 55 ± 5%, 12 hours light-dark cycle) were supplied free of distilled water and a standard diet as described above.

Experimental Example 3: Liposuction Surgery

Fifteen rats with an average body weight of about 300 g were used for the development of a fat absorption tube model, five per group. Previous studies by the lead investigator (Noh et al., 1999; Noh et al., 2001a &200b; Loest, 2002; Noh et al., 2003a &2003b; Noh et al., 2004; Shu & Noh, 2006; In the antiseptic conditions described in detail in Zou et al. (2005) (FIG. 1), individual animals are dissected from the abdominal midline after anesthesia is maintained by carburettor (isoflurane / oxygen) one by one on the day of the experiment. It was. Lymph sample and duodenal infusions took about 40 minutes per horse.

According to lymph duct cannulation microsurgery, PE (polyethylene) tubing (lymph sample tube SV 31, USA) was inserted into the major mesenteric lymph duct and fixed. When ingested, the dietary fat that enters the small intestine enters the lymphatic tract from the small intestine. Thus, in this experiment, this bypass tube is a sampling tube for directly quantifying the fat absorbed through the small intestine during the absorption experiment and obtaining a lymph sample for analyzing various fat components.

An intra-duodenal catheter (OD 2.1 mm, USA) is used to quantitatively measure the rate of absorption of the small intestine and to accurately inject the precisely calculated and refined lipid emulsion into the small intestine (duodenum). The upper part of the small intestine, that is, a kind of fatty emulsion injection tube inserted to enter the duodenum. Thus, the tube was inserted and fixed in the duodenum 4 cm through the gastric fundus of the stomach with little blood vessels. After these tubes were fixed, each tube (lymph sampling tube and duodenal infusion tube) was penetrated through the right flank and then fixed.

And after limiting the animals operated in the restraining cage, after a recovery time of about 20 hours in a recovery chamber maintained at about 30 ° C., in-vivo in vivo. Was carried out. During this recovery period, a continuous duodenal injection of PBS (phosphate buffered saline) containing 5% glucose (6.75 mM Na ₂ HPO ₄ , 16.5 mM NaH ₂ PO ₄ , 115 mM NaCl, and 5 mM KCl, pH 6.4) 3 mL per hour was injected into the intra-duodenal catheter. 5% glucose was used to promote recovery and PBS solution was used to prevent dehydration and mineral supplementation. The next morning, just before the absorption experiment, a precisely formulated lipid emulsion was added to the intra-duodenal catheter at 3 mL per hour, and a 24 mL fat emulsion was continuously maintained for 8 hours. NE-1600, New Era Pump Systems, Inc., NY, NY, USA). Lymph samples were taken at the same time into the lymph sample collection tube while injecting 3 mL per hour for 8 hours. The composition of the injected emulsion is shown in Table 2, the basic composition is 33.3kBq [ ¹⁴ C] -cholesterol (specific activity, 1.9GBq / mmol, Perkin Elmer, Life and Analytical Sciences, MA, USA), 451.8μmol triolein (95%; Sigma Chemical), 3.1 μmol α-tocopherol (all-rac-α-tocopherol, 97%; Aldrich Chemical), 75.4 nmol retinol, 396 μmol sodium taurocholate and 20 μmol cholesterol]. In order to check the proportional effect, the control group was divided into three groups, and the control group did not add sorghum extract, and the low sorghum extract group received 80 mg / s. 8 h (10 mg / h) high sorghum extract administration group was added 240 mg / 8 h (30 mg / h) to the fat emulsion, and the other additives were prepared and administered under the same conditions. As representative dietary fats, cholesterol and triolein were used, and these concentrations were generally calculated at the level of daily intake of each fat. α-tocophenol and retinol are representative fat-soluble vitamins. In addition to the nutritional aspects, it was included as an indicator to check the degree of fat solubility, ie, how the carbon number affects the absorption stage, in the absorption process.

Fat emulsion composition content Fat emulsion composition content ¹⁴ C-cholesterol 1.0 μCi α-Tocopherol 3.1μmol Cholesterol 8.0μmol Retinol 75.4nmol Triolein 451.8μmol Albumin 72.0mg Na-taurocholate 396.0μmol PBS buffer (pH 6.4) 24.0 mL

Experimental Example 4: Measurement of ¹⁴ C-cholesterol Radiation

The amount of radiation was measured using a liquid scintillation counter (Wallac 1414, Perkin Elmer, Inc, Life and Analytical Sciences, MA, USA) as a lymph sample. The absorption rate is 100% of the total amount of lipid emulsion ¹⁴ C injected based on the amount of radiation (DPM) per 100 μL lymph sample, and is absorbed into the lymph cannula bypassed in vitro. The amount secreted (DPM) was compared and the absorption rate (% dose / 8h) for 8 hours was calculated. 100 μL lymph samples were mixed with 10 mL of scintillation aid (Ready safe ^™ , Beckman Coulter. Inc., CA, USA) and measured using a liquid scintillation counter. The remaining lymph samples were stored at -70 ° C until analysis of other fat components.

Experimental Example 5 Simultaneous Analysis of α-tocopherol and Retinol in Lymphatic Fluid Using HPLC

Lymphoid fat was first extracted from 100 μL lymph sample by chloroform: methanol (v / v, BHT 151.3 μmol / L) mixed organic solvent method. Tocol (Matreya, USA) was used as an internal standard and after separation of the organic solvent layer, the lower solvent was removed using an N ₂ concentrator (N-EVAPTM 111, Organomation Associates Inc., MA, USA). Simultaneous measurement of α-tocophenol and retinol was performed under the following conditions. The HPLC column used was a reversed phase column (Alltima C18, 5 μm, 4.6 × 150 mm, Alltech Associates, Inc., IL, USA) maintained at 40 ° C., Beckman System Gold software (Beckman Instruments, Inc., Fullerton, CA, USA) Was connected to the device. The mobile phase was 100% methanol (2 mL / min) and analyzed at UV 295 nm. Total cholesterol analysis was analyzed using HPLC (Noh et al., 1999; Noh et al., 2001a &200b; Loest, 2002; Noh et al., 2003a &2003b; Noh et al., 2004; Shu & Noh , 2006; Zou et al., 2005). The amount contained in lymphatic phospholipids was determined by chemical methods (Noh et al, 1999).

Experimental Example 6 Analysis of Lymphatic Fatty Acid Using GC

Total fatty acid in lymph fluid was measured according to the method of Folch et al. And Slover et al. 100 μL of lymphatic fluid was taken and extracted with cholroform: methanol (v / v, 2: 1) mixed solvent, followed by addition of 2 mL of 0.5N methanolic NaOH and 14% BF ₃ to induce methylation. The isolation and identification of fatty acids was measured using a GC (Model GC 7890A, Agilent Technologies, Inc., Wilmington, DE, USA) and a flame ionization detector. The column used was DB-23 (50% -Cyanopropyl) -methyl polysiloxane; 0.15μm, 0.2mm, 60m, Agilent Inc., USA), the separation temperature conditions were measured for 10 minutes at 200 ° C, then 5 ℃ at 1 minute and then 4 minutes at 220 ℃ for 9 minutes. The internal standard used was 17: 0 and the amount of each fatty acid was calculated in comparison with standard fatty acids (Nu-Chek Prep Inc., Elysian, MN, USA) under the same analytical conditions.

Experimental Example 7 Food Addition of Sorghum Extracts Efficacy in Inhibiting Small Intestine Fat Absorption Rate

Examples of the food to which the extract or fraction having the effect of inhibiting small intestine fat absorption may be added, for example, drink, meat, sausage, bread, chocolate, candy, snacks, confectionary, pizza, ramen, gum, ice cream, soup, Beverages, alcoholic beverages and vitamin complexes, but are not limited thereto. At this time, the amount of the extract or fraction in the food or beverage may be added in 0.01 to 15% by weight of the total food weight, the health beverage composition may be added in a ratio of 0.02 to 5g, preferably 0.3 to 1g based on 100ml. It is good to adjust according to the type of food and the method of use.

Example 1 Lymph Secretion Rate

When the lipid emulsion containing sorghum extract was continuously injected into the duodenum of the experimental animal for 8 hours, the amount of various fats or lymph introduced into the lymph circulation system for 8 hours at the same time is shown in Table 3. The amount of lymph secreted by sorghum extract was significantly reduced compared to the control. The secreted amount tended to increase continuously in the control group, but the increase rate by the sorghum extract in the experimental group tended to decrease in the control group regardless of the dose of sorghum extract. The total amount of lymph secreted for 8 hours was 23.08 mL in the control group and 17.12 mL and 14.71 mL in the sorghum extract group, respectively.

Lymph lipids Control group Low concentration sorghum extract group High concentration sorghum extract group Lymph (mL / 8h) 23.08 17.12 14.71 ¹⁴ C-CH (% dose / 8 h) 39.08 25.81 19.66 total CH (μmol / 8h) 18.44 13.34 10.48 αTP (nmol / 8h) 1,184.90 809.00 753.40 (% dose / 8h) 37.81 26.05 24.04 Retinol (nmol / 8h) 13.60 14.97 19.22 (% dose / 8h) 18.04 19.86 25.50 Total FA (μmol / 8h) 1,709.00 1,515.00 1,342.00 PL (μmol / 8h) 36.45 36.66 36.45

Example 2 Small Intestine ¹⁴ C-cholesterol Absorption

The amount (absorption rate) of ¹⁴ C-cholesterol introduced into the small intestine lymph circulation system when the fat emulsion containing sorghum extract was injected into the duodenum through the stomach for 8 hours is shown in FIG. 2. The ¹⁴ C-cholesterol uptake rate was not significantly different between control and sorghum extract animals between the first 1-2 hours, but the absorption rate of ¹⁴ C-cholesterol in the control group increased rapidly from 3 hours after the sorghum extract administration. On the other hand, the absorption rate of the sorghum extract-treated animals started to decrease significantly, and from 4 hours, the increase rate was the slowest in the group receiving more sorghum extracts and decreased to a significant difference. . Finally, the total amount of ¹⁴ C-cholesterol absorbed for the total 8 hours was 39.08% dose in the control group and 25.81% dose in the low sorghum extract group, which was a significant difference. In the high sorghum extract-treated animals, the absorption rate of ¹⁴ C-cholesterol for 8 hours was 19.66% dose, and it was found that the sorghum extract inhibited the absorption of cholesterol in the small intestine by 33.8 and 49.7%, respectively (Table 3). .

Example 3 Effect of Sorghum Extracts on the Small Intestine Absorption Rate of Fat-Soluble Vitamin α-Tocophenol

When the fat emulsion containing sorghum extract was injected into the small intestine (duodenum), the absorption rate of α-tocophenol introduced into the lymph circulation system of the small intestine is shown in FIG. 3. In the present invention, the reason for measuring the absorption rate of α-tocophenol together was firstly to examine how sorghum extract affects the absorption of vitamin E, a representative micronutrient, and secondly, the molecular formula of α-tocophenol is C. ₂₉ H ₅₀ O ₂ is similar to the above-mentioned cholesterol, similar in molecular structure and carbon number, and polar solubility. In addition, essential fat-soluble antioxidant vitamins were included in the lipid emulsion to see if they interact with each other during digestion and absorption. As shown in FIG. 3, the sorghum extract administered animals group tended to be significantly lower than the control group from 2 hours to 8 hours, and there was no effect according to the dose of sorghum extract. The total amount of α-tocophenol secreted for 8 hours was calculated to be 1,184.9 nmol (37.81% dose), 809.0 nmol (26.05% dose), 753.4 nmol (24.04% dose) in control, low sorghum extract and high sorghum extract ( Table 3). α-tocophenol is a fat-soluble component and a cholesterol-like substance as can be seen from the above results. Thus, it is absorbed into the lymph circulation system through a cholesterol-like absorption pathway. In general, the absorption path is mainly affected by the lipid components, so the tendency to suppress absorption by polyphenols including flavonoids, which are mainly vegetable extracts, was also confirmed in this experiment. The results of this experiment suggest that, similar to the absorption rate of cholesterol, similar polar solubility α-tocophenol is also suppressed and special care is required for nutritional management of fat-soluble trace elements when eating sorghum in the long term. However, the various polyphenolic constituents of sorghum are antioxidative as much as this vitamin E, which is expected to replace the homeostasis as sufficiently as vitamin E affected. I think this will be.

Example 4 Effect of Sorghum Extract on Small Absorption Rate of Vitamin A (retinol, ROH)

The effect of sorghum extract on the small intestine absorption of vitamin A (retinol) as another micronutrient was investigated. When the fat emulsion containing the sorghum extract was injected into the small intestine (duodenum), the absorption rate of the retinol introduced into the small intestine fat absorption tube is shown in FIG. 4. The results of the retinol uptake are intriguing with the results of the cholesterol uptake. The reason why the absorption rate of vitamin A (retinol) was measured together was to examine the effects on vitamin E and the most representative micronutrients. As shown in Figure 4, starting from the administration of sorghum extract there was no difference in the comparison between groups until 5 hours, but the absorption rate began to increase rapidly with a significant difference only in the high sorghum group from 6 hours It was. The total amount of retinol absorbed for 8 hours was 13.60 nmol (18.04% dose), 14.97 nmol (19.86% dose) and 19.22 nmol (25.50% dose) in the control, low and high sorghum extract animals groups (Table 3). Retinol, like the α-tocophenols described above, is a representative fat-soluble vitamin and enters the lymphatic system and is affected by various dietary factors. Contrary to expectations, however, the tendency to increase by sorghum extract has several important meanings. Vitamin A is an important nutrient that affects cell growth, visual acuity, and gene expression. The results suggest that sorghum extract may be an important source of vitamin A while reducing the absorption of cholesterol, a factor in adult disease. Based on these results, the application aspect is considered to be an important clue for commercialization or patentization. It is also believed that vitamin A may be a scientific basis for explaining important dietary prescriptions for adolescents and the elderly who are in need of growth.

Example 5 Effect of Sorghum Extract on Small Absorption Rate of Fatty Acids

The effect of sorghum extract on the absorption rate of small intestine, triglycerides, consisting of three molecules of fatty acids and one molecule of glycerol, was investigated. Absorption rate of fatty acids introduced into the small intestine fat absorption tube when sorghum extract and triolein containing fat emulsion were injected into the small intestine (duodenum) is shown in Table 4. Triolein was used as a representative triglyceride. Triolein uptake was not significantly different between control and low-marrow sorghum-treated animals, as measured by oleic acid, but significantly reduced absorption of control group and high-sorghum extract-treated animals. Finally, the amount of oleic acid absorbed for 8 hours was 1427.3 μmol / 8h in the control group, 1282.4 μmol / 8h in the low concentration sorghum extract group, and 1122.4 μmol / 8h in the high sorghum extract group (Table 4). . Total fatty acids, combined with all types of fatty acids entering the lymphatic circulatory system, whether triolein or endogenous, also showed a similar inhibition pattern to oleic acid absorption.

Lymphatic Fatty Acid Composition Control group
(μmol / 8h) Low concentration sorghum extract group
(μmol / 8h) Low concentration sorghum extract group
(μmol / 8h) 16: 0 99.07 82.52 75.32 18: 0 38.85 32.54 31.36 18: 1 1,427.30 1,282.40 1,122.40 18: 2 85.60 70.12 69.36 20: 4 50.71 41.71 38.90 22: 6 7.39 5.52 5.06 Total amount 1,708.90 1,514.80 1,342.40

Example 6 Effect of Sorghum Extract on Phospholipid Secretion Rate

Phospholipids were not included in fat emulsions. In other words, the results of this phospholipid analysis are related to endogenous lipids. The sorghum extract administration can confirm the effect on the metabolism of endogenous fat and not dietary fat during the absorption process (Fig. 5). Compared with the control group, there was no effect on the secretion rate of endogenous phospholipids for 8 hours regardless of whether there was more or less sorghum extract.

Claims

A pharmaceutical composition for treating hyperlipidemia and inhibiting intestinal fat absorption using an extract extracted from red sorghum as an active ingredient.

Sorghum extract effective for inhibiting hyperlipidemia and small intestine fat absorption, which is characterized by pulverizing sorghum of red series, extracting the active ingredient with an organic solvent, and concentrating under reduced pressure and lyophilization.

Cholesterol and fat absorption inhibitory health food containing red sorghum extract as an active ingredient.

4. The dietary supplement for inhibiting cholesterol and fat absorption according to claim 3, which is a powder, granule, tablet, capsule, syrup or beverage utilizing red sorghum extract that has not been milled.

A method for inhibiting small intestine absorption of ¹⁴ C-cholesterol and triglycerides by an intestinal mesenteric lymph duct cannulated rat model using an extract extracted from red sorghum as an active ingredient.

A small intestine absorption method of fat-soluble vitamin α-tocophenol and vitamin A by an intestinal mesenteric lymph duct cannulated rat model using an extract extracted from red sorghum as an active ingredient.