KR20130003401A - Standard marker for determining place of origin or age of processed ginseng, establishing method thereof, or method for determining place of origin or age using the same - Google Patents

Abstract

PURPOSE: A standard marker for determining origin or age of processed ginseng and a method for determining the origin or age are provided to quickly analyze and compare the origin or age of the processed ginseng such as a concentrate liquid and a power. CONSTITUTION: A marker of chemical formula 1 or 2 for determining origin of raw ginseng is used for distinguishing domestic ginseng and Chinese ginseng. A compound of chemical formula 1 is a marker for identifying domestic ginseng. A compound of chemical formula 2 is a marker for identifying Chinese ginseng. A method for establishing the marker comprises: a step of extracting a metabolite from the domestic ginseng and Chinese ginseng; a step of analyzing the metabolite by a liquid chromatography-mass spectrometer(LC-MS); and a step of detecting ingredients with difference between two groups and selecting an ingredient with largest difference.

Description

Standard marker for determining place of origin or age of processed ginseng, establishing method approximately, or method for determining place of origin or age using the same}

The present invention relates to a standard marker for determining the origin or age of processed ginseng, a method for establishing the same or a method for determining the origin or age using the same.

In recent years, non-destructive techniques such as near-infrared spectroscopy, electronic nose, and X-ray fluorescence spectroscopy have been widely used. In particular, nondestructive techniques using near-infrared spectroscopy are used to determine the sugar content of fruit vegetables, the moisture content of cereals, Protein and solids content of milk and dairy products and it is possible to utilize existing protein analysis methods such as electrophoresis and HPLC method in order to discriminate the kind or origin of agricultural products, And there was difficulty in application in the field. Recently, however, CE chromatograms have been applied as a new technique capable of quantitative digital analysis and shorter analysis time due to shorter analysis time and higher resolution than conventional methods. It is mainly used for breed identification (Nam Soo Kim, "Development of Discrimination Technology and Safety Evaluation Technique for Domestic and Imported Agricultural Products (Processed Foods)" Research Project Report, 2003).

Therefore, it is possible to efficiently classify the difference in quality caused by the production area or cultivation conditions of various agricultural products including processed ginseng and unprocessed ginseng, and to develop scientific discrimination methods to meet the strengthening and institutionalization of agricultural labeling by the WTO system. As there is an increasing number of imported and illegally imported agricultural products, there is a need to develop a scientific identification method for verifying the origin of these agricultural products collected in the process of on-site enforcement.

Ginseng (panax ginseng C.A. Meyer) has been used for thousands of years as a medicinal plant, and ginseng has long evolved to fit its environment. Little by little, they have adapted themselves to live well in their environment and have changed themselves according to their environment.Ginseng is divided into ginseng, white ginseng and red ginseng according to the processing method, and the ginseng of mined natural state is called ginseng. Peeled and dried in sunlight or hot air is called white ginseng. In addition, red ginseng is steamed and dried without steaming white ginseng (red ginseng).

Korean ginseng has been known to be the best among Korean ginseng. In recent years, however, international markets have been encroached on cheap Chinese and American and Canadian ginseng, and Chinese ginseng has recently entered the domestic market through wheat trade, making it difficult to position domestically grown ginseng. Study on the Establishment and Efficacy of Classification System of Wild Ginseng and Chinese Camphor Ginseng ”Research Project Report, 2007) Also, overseas ginseng does not have much meaning for lotus root discrimination because it is mined for 3-4 years old ginseng. According to the customary belief that the better the drug is, the older the roots are, the more expensive they are, and so the cases of disguising the lower roots as 6 years old in the distribution market frequently cause disturbances in the distribution order and low consumer confidence. Discrimination is a big problem.

At present, the inspection standard for the determination of lotus root by the Ministry of Agriculture and Forestry is determined by the inspecting staff at the time of harvest, and the lotus root is judged by detailed inspection.The detailed criteria is determined by visually or coloring the head, trunk and epidermis, the degree of leg development, and the ring during cutting. Industrial Law, Article 8, 2004), which is used as an auxiliary discrimination method in the field where the cultivation history is confirmed, but it is difficult to apply in the distribution market where the level of sensory evaluation is controversial, and the cultivation history is unclear. It can be said. The researches reported on the lotus root scarring (J. Ginseng Res. Vol. 31, 142-146, 2007), Nayte (J. Ginseng Res. Vol 30, 153-157, 2006, Ginseng Research Report-cultivation, Korean Ginseng and Tobacco Research Institute, p331, 1987), Geun-Gi-Development (Ginseng Research Report-Recultivation, Korean Ginseng and Tobacco Research Institute, p175, 1988), Machine Drying Method (J.Ginseng Res. Vol 30, 153-157, 2006), Tissue Dyeing (J. Ginseng Res. Vol 25, 101-105, 2001) and ginsenoside content (Can. J. Plant Sci. Vol 76, p 855 1996) have been reported, but secretory conduit tissue staining is an accurate method. It is evaluated and other methods are low level of reliability and are used as auxiliary. However, the secretory conduit tissue staining method is limited to cultivated ginseng and is not applicable to ginseng powder, and is difficult to apply to processed red ginseng because it is distinguished by root section of root.

In order to solve the above problems, the present inventors are studying the origin or lotus root discrimination method of domestic processed ginseng and Chinese processed ginseng, separating and analyzing the standard markers from domestic processed ginseng and Chinese processed ginseng, and selecting them using the same. Or it was confirmed that the lotus root can be identified and completed the present invention.

An object of the present invention is to provide a marker for determining the production area of white ginseng used to determine the production of white ginseng.

Another object of the present invention is to provide a method for determining a mountain of white ginseng.

Still another object of the present invention is to provide a marker for determining a red ginseng producing region, which is used for determining the area of red ginseng.

Another object of the present invention is to provide a method for determining the production of red ginseng.

Another object of the present invention is to provide a marker for determining the age of red ginseng used to determine the age of red ginseng.

Another object of the present invention is to provide a method for determining the age of red ginseng.

In order to achieve the above object, the present invention provides a marker for determining the production location of white ginseng represented by the following Chemical Formula 1 or 2 used for the production of domestic white ginseng and Chinese white ginseng:

[Formula 1]

[Formula 2]

In addition, the present invention comprises the steps of extracting a metabolite from the test white ginseng (step 1);

Analyzing the metabolite extracted in step 1 by liquid chromatography-mass spectrometry (LC-MS) (step 2); And

It provides a method for determining the mountain area of white ginseng comprising the step (step 3) of comparing the LC-MS analysis result obtained in step 2 with the marker for determining the mountain region of Formula 1 or 2.

Furthermore, the present invention provides a marker for determining the origin of red ginseng represented by the following Chemical Formula 3 or 4, which is used for the determination of the production of Korean red ginseng and Chinese red ginseng:

(3)

[Formula 4]

In addition, the present invention comprises the steps of extracting a metabolite from the test red ginseng (step 1);

Analyzing the metabolite extracted in step 1 by liquid chromatography-mass spectrometry (LC-MS) (step 2); And

It provides a method for determining the location of red ginseng comprising the step (step 3) of comparing the LC-MS analysis result obtained in step 2 with the acid determination marker of the formula (3) or (4).

Furthermore, the present invention provides a marker for determining the age of red ginseng represented by the following formulas 5 to 11 used for the discrimination of lotus root of Korean four-year-old red ginseng and six-year-old red ginseng:

[Chemical Formula 5]

[Formula 6]

[Formula 7]

[Formula 8]

[Chemical Formula 9]

[Formula 10]

[Formula 11]

In addition, the present invention comprises the steps of extracting a metabolite from the test red ginseng (step 1);

Analyzing the metabolite extracted in step 1 by liquid chromatography-mass spectrometry (LC-MS) (step 2); And

It provides a method for determining the age of red ginseng comprising the step (step 3) comparing the LC-MS analysis obtained in step 2 with an age discriminating marker selected from the group consisting of the compounds represented by Formulas 5 to 11.

According to the present invention, a standard marker is established in domestic processed ginseng, that is, white ginseng and red ginseng, and the comparative analysis of the origin or age of processed ginseng, such as concentrate or powder, which is difficult to identify the origin or age using the markers, is performed. Therefore, when importing and exporting, it can be useful for determining the origin or age of processed ginseng.

1 is a view showing an ion chromatogram of domestic white ginseng extract of the embodiment of the present invention.
Figure 2 is a view showing an ion chromatogram of Chinese ginseng extract of the embodiment of the present invention.
Figure 3 is a diagram showing a score scatter plot of domestic white ginseng extract and Chinese white ginseng extract of the embodiment of the present invention.
Figure 4 is a view showing the S plot of the least orthogonal square analysis of domestic white ginseng extract and Chinese white ginseng extract of the embodiment of the present invention.
Figure 5 is a view showing a box plot of (a) domestic white ginseng extract and (b) Chinese white ginseng extract of the embodiment of the present invention.
Figure 6 is a view showing the mass cutting method of domestic white ginseng extract of the embodiment.
7 is a view showing a mass cutting method of the Chinese white ginseng extract of the embodiment.
8 is a diagram showing an ion chromatogram of domestic red ginseng extract of the embodiment of the present invention.
9 is a view showing an ion chromatogram of Chinese red ginseng extract of the embodiment of the present invention.
10 is a view showing a score scatter plot of the domestic red ginseng extract and the Chinese red ginseng extract of the embodiment of the present invention.
11 is a view showing the S plot of the least-orthogonal square analysis of domestic red ginseng extract and Chinese red ginseng extract of the embodiment of the present invention.
12 is a view showing a box plot of (a) domestic red ginseng extract and (b) Chinese red ginseng extract of the embodiment of the present invention.
13 is a view showing the mass cutting method of the domestic red ginseng extract of the embodiment.
14 is a view showing the mass cutting method of the Chinese red ginseng extract of the embodiment.
15 is a diagram showing an ion chromatogram of 4 years old Korean red ginseng extract of the Example of the present invention.
16 is a diagram showing an ion chromatogram of 6 years old Korean red ginseng extract of the Example of the present invention.
17 is a diagram showing a score scatter plot of domestic four-year-old red ginseng extract and six-year-old red ginseng extract of the embodiment of the present invention.
FIG. 18 is a diagram showing a S-plot of a minimum orthogonal square analysis of 4-year old Korean red ginseng extract and 6-year old green ginseng extract of the present invention.
19 is a diagram showing a box plot of the domestic four-year-old red ginseng extract of the embodiment of the present invention.
20 is a view showing a box plot of the domestic six-year-old red ginseng extract of the embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

The present invention provides a marker for determining the production location of white ginseng represented by the following formula (1) or 2 used for the production of domestic white ginseng and Chinese white ginseng:

At this time, the compound represented by the formula (1) is a marker for determining the production of domestic white ginseng, the compound represented by the formula (2) is a marker for determining the production of white ginseng from China.

Marker for determining the production site of white ginseng represented by the formula (1) or 2 according to the present invention comprises the steps of extracting a metabolite from domestic white ginseng and Chinese white ginseng (step 1);

Analyzing the metabolite extracted in step 1 by liquid chromatography-mass spectrometry (LC-MS) (step 2); And

In step 2, the result analyzed by LC-MS is converted into a numerical value that can be statistically processed, and the data obtained by analyzing the converted value by the multivariate analysis method are detected. It can be established in a method comprising the step (step 3) of establishing as a standard marker according to the mountain.

Specifically, step 1 according to the present invention is a step of extracting metabolites from domestic white ginseng and Chinese white ginseng, and the extraction solvent is preferably water, alcohol or a mixture thereof. As the alcohol, C ₁ to C ₄ lower alcohols are preferably used, more preferably ethanol or methanol, and most preferably 70% methanol, but not always limited thereto. Examples of the extraction method include filtration, hot water extraction, immersion extraction, reflux cooling extraction, ultrasonic extraction, and the like, and the extraction is preferably performed once to five times by the ultrasonic extraction method, But is not limited thereto. Extraction temperature is preferably 20 to 30 ℃ but is not limited thereto.

In addition, step 2 is a step of analyzing the metabolite extracted in step 1 by liquid chromatography-mass spectrometry (LC-MS), column chromatography was used at 30-40 ℃, wherein the mobile phase A is available Water and formic acid (100: 0.1, v / v) and mobile phase B were used as acetonitrile (acetonitril) and formic acid (100: 0.1, v / v). The flow rate of the mobile phase is 0.4 ml / min and the sample injection amount can be used for the measurement by taking 1 μl of each sample of domestic white ginseng extract and Chinese white ginseng extract. In addition, the mass spectrometer may use a quadrupole orthogonal accelerated flight time tandem mass spectrometer, but is not limited thereto.

Further, Step 3 converts the result analyzed by LC-MS into a numerical value that can be statistically processed, and analyzes the converted value by multivariate analysis to detect a component having a difference between groups from the data, and then selects the component having the largest difference. By establishing the standard markers of domestic white ginseng standard markers and Chinese white ginseng as standard markers according to the regions, the multivariate analysis used at this time is an orthogonal least-squares analysis, and the data obtained by analyzing the multivariate analysis is a score scatter plot. Standard markers can be identified.

In addition,

Extracting the metabolite from the test white ginseng (step 1);

Analyzing the metabolite extracted in step 1 by liquid chromatography-mass spectrometry (LC-MS) (step 2); And

It provides a method for determining the mountain area of white ginseng comprising the step (step 3) of comparing the LC-MS analysis result obtained in step 2 with the marker for determining the mountain region of Formula 1 or 2.

The production method of white ginseng can be used to determine the production location of white ginseng when importing and exporting, because it is possible to quickly compare the production area of white ginseng, such as concentrate, powder, etc. difficult to identify the mountain.

Furthermore, the present invention provides a marker for determining the origin of red ginseng represented by the following Chemical Formula 3 or 4, which is used for the determination of the production of Korean red ginseng and Chinese red ginseng:

At this time, the compound represented by the formula (3) is a marker for discriminating the production site of Korean red ginseng, and the compound represented by the formula (4) is a marker for determining the production site of Chinese red ginseng.

Further, the markers for discriminating the origin of red ginseng represented by the above formula (3) or (4) according to the present invention may include a step (step 1) of extracting metabolites from Korean red ginseng and Chinese red ginseng;

Analyzing the metabolite extracted in step 1 by liquid chromatography-mass spectrometry (LC-MS) (step 2); And

In step 2, the result analyzed by LC-MS is converted into a numerical value that can be statistically processed, and the data obtained by analyzing the converted value by the multivariate analysis method are detected. It can be established in a method comprising the step (step 3) of establishing as a standard marker according to the mountain.

Specifically, the steps 1 to 3 may be performed by the same method as the method for determining the production of the white ginseng, but is not limited thereto.

In addition,

Extracting the metabolite from the red ginseng to be examined (step 1);

Analyzing the metabolite extracted in step 1 by liquid chromatography-mass spectrometry (LC-MS) (step 2); And

It provides a method for determining the location of red ginseng comprising the step (step 3) of comparing the LC-MS analysis result obtained in step 2 with the acid determination marker of the formula (3) or (4).

The method for determining the production location of red ginseng can be used to determine the production location of red ginseng at the time of import and export, since it is possible to quickly compare the production area of red ginseng such as concentrate, powder, etc. which are difficult to identify.

Furthermore, the present invention provides a marker for determining the age of red ginseng represented by the following formulas 5 to 11 used for the discrimination of lotus root of Korean four-year-old red ginseng and six-year-old red ginseng:

At this time, the compound represented by the formula 5 to 8 is a marker for determining the age of 4 years old red ginseng, the compound represented by the formula 9 to 11 is a marker for determining the age of 6 years old red ginseng.

The age-determining markers of red ginseng represented by Chemical Formulas 5 to 11 according to the present invention include the steps of extracting metabolites from domestic 4 year old red ginseng and 6 year old red ginseng (step 1);

Analyzing the metabolite extracted in step 1 by liquid chromatography-mass spectrometry (LC-MS) (step 2); And

In step 2, the result analyzed by LC-MS is converted into a numerical value that can be statistically processed, and the data obtained by analyzing the converted value by the multivariate analysis method are detected. It can be established by a method comprising the step of establishing as a standard marker according to the age of red ginseng (step 3).

Specifically, the steps 1 to 3 may be performed by the same method as the method for determining the production of the white ginseng, but is not limited thereto.

In addition,

Extracting the metabolite from the red ginseng to be examined (step 1);

Analyzing the metabolite extracted in step 1 by liquid chromatography-mass spectrometry (LC-MS) (step 2); And

It provides a method for determining the age of red ginseng comprising the step (step 3) comparing the LC-MS analysis obtained in step 2 with an age discriminating marker selected from the group consisting of the compounds represented by Formulas 5 to 11.

The age determination method of the red ginseng can be used to determine the age of the red ginseng at the time of import and export, since it is possible to quickly compare the age of the red ginseng, such as concentrate, powder, etc., difficult to identify the age using a standard marker.

Hereinafter, the present invention will be described in detail by the following examples.

However, the following examples are illustrative of the present invention, and the contents of the present invention are not limited by the following examples.

< Manufacturing example  1> Preparation of Processed Ginseng

Regardless of the age of lotus root (age), 53 Korean white ginseng from Korea were purchased from Korea to determine the origin of Korean and Chinese white ginseng, 18 Chinese white ginseng from Korea and from China, and also from Korea and Chinese red ginseng. 50 Korean red ginseng were purchased in Korea and 35 Chinese red ginseng were purchased in China and Korea. In addition, red ginseng was prepared from 4 ginseng 10 roots and 6 ginseng 10 roots ginseng from Icheon, Gyeonggi-do.

< Example  1> Standard for producing mountainous ginseng Marker  Establish

The following experiment was performed to establish markers for determining the origin of domestic white ginseng and Chinese white ginseng.

Step 1: white ginseng Metabolic  extraction

After drying domestic white ginseng and Chinese white ginseng prepared in Preparation Example 1, each was pulverized, 0.2 mg of each was added, 70 ml of 70% methanol was added, and an ultrasonic degradation apparatus of 350 W was irradiated for 1 hour, and then the extract was 0.2 μm. The extract was collected by filtration through a membrane filter (SUN-SRi, TN, USA).

Step 2: Liquid Chromatography-Mass Spectrometry LC - MS Analysis of White Ginseng Extract Using

In order to analyze the metabolites of the white ginseng extract prepared in step 1, UPLC used a Waters Equity UPLC System, and the column was EQUITY ™ BEHC ₁₈ (ACQUITY UPLC ™ BEHC ₁₈ ) (10 mm × 2.1 mm, id). , 1.7 μm, waters, USA) was used at 35 ° C., mobile phase A was water and formic acid (100: 0.1, v / v), and mobile phase B was acetonitrile (acetonitril) and formic acid (100: 0.1, v / v). Initially, mobile phase B was increased from 10% to 30% for 7 minutes, 44% to 14 minutes, 100% to 21 minutes, and then mobile phase B was analyzed for 23.5 minutes to 100%. Until the mobile phase B was lowered to 10% at 23.6 minutes, then equilibrated and used for the next analysis. The flow rate of the mobile phase was 0.4 ml / min, and the sample injection amount was 1 μl each of domestic white ginseng extract and Chinese white ginseng extract.

The Q-TOF mass spectrometer used Waters' quadrupole orthogonal acceleration time of-flight tandem mass spectrometer (Waters Q-TOF Premier ™). Analysis conditions of the mass spectrometer are as shown in Table 1 below.

Ion source ESI + Nebulizer gas 500 L / h Nebulizer Temperature 350 ℃ Con gas 50 L / h Source temperature 100 ℃ Capillary voltage 2.7 kV Con Voltage 27 V

The total ion chromatogram of liquid chromatography-mass spectrometry (LC-MS) of the domestic white ginseng extract and the Chinese white ginseng extract is shown in FIGS. 1 and 2.

Step 3: convert to statistical numbers and white ginseng standard Marker  Establish

In order to convert the LC-MS data obtained in step 2 into numerical values capable of being statistically processed, peak finding and peak alignment are performed using Waters Markerynx applications manager (ver. 4.1). And peak filtering was performed. For the above process, 0.5 to 19 minutes of analysis data were used, analytical mass range was 200-1500 Da, mass error tolerance was 0.02 Da, and mass noise level was the base peak intensity. Was 10%. In all LC-MS data obtained through this process, the retention time (RT) value and the mass value of each peak were matched, and the area value of each peak was obtained. Then, for multivariate statistical analysis, Simca-P + ver. Using the 12.0 (Umetrics, Sweden) software, all data are par-scaled (scaled to square root of SD), grouping domestic and Chinese white ginseng to score scores of orthogonal least squares analysis (OPLS-DA). Cat plots were shown, and the S plot (Simca-P +) of orthogonal least-squares analysis was taken to represent box-plots of components with high differences between the two mountain regions to select natural peaks for discrimination and their mass ion chromatograms [mass] Ion chromatogram, MassLynx (ver 4.1)] was compared to identify the structure of the significantly different components by mass fragmentation pattern (mass fragmentation pattern) to identify the structure. The results are shown in FIGS. 3 to 7.

result

(1) As a result of the score scatter plot of the orthogonal least-squares analysis, as shown in FIG. 3, domestic white ginseng and Chinese white ginseng were separated into two groups according to the mountain region.

(2) In addition, as a result of the S plot of the orthogonal least-squares analysis, as shown in FIGS. 4 and 5, there is a significant difference between a component having a mass of 963.5582 for 5.21 and a component having a mass of 771.4919 for 9.30. (T-test; p> 0.001).

(3) Furthermore, as a result of the mass fragmentation pattern, as shown in FIGS. 6 and 7, the standard marker of domestic white ginseng was 20-glucosinenoside Rf (5.21 min, MW), as shown in the following formula (1). 963.5582), and the standard marker of Chinese ginseng was identified as notogincetoside R2 (9.30 min, MW 771.4919), as shown in the following Chemical Formula 2.

      [Formula 1] [Formula 2]

< Example  2> Standards for the Production of Red Ginseng Marker  Establish

The following experiment was conducted to establish markers for determining the location of Korean red ginseng and Chinese red ginseng.

Step 1: red ginseng Metabolic  extraction

Except for using domestic red ginseng and Chinese red ginseng prepared in Preparation Example 1 was carried out in the same manner as in Step 1 of Example 1.

Step 2: Liquid Chromatography-Mass Spectrometry LC - MS Analysis of Red Ginseng Extract Using

Except for using the metabolite of the red ginseng extract prepared in step 1 was carried out in the same manner as in step 2 of Example 1.

Results of the total ion chromatogram of the liquid chromatography-mass spectrometry (LC-MS) of the domestic red ginseng extract and the Chinese red ginseng extract are shown in FIGS. 8 and 9.

Step 3: Convert Statistical Numbers to Processable Red Ginseng Standards Marker  Establish

The same procedure as in Example 3, except that the results obtained in step 2 were used.

result

(1) As a result of the score scatter plot of the orthogonal least-squares analysis, as shown in FIG. 10, domestic red ginseng and Chinese red ginseng were separated into two groups according to the mountain region.

(2) In addition, as a result of the S plot of the orthogonal least squares analysis, as shown in FIGS. 11 and 12, there is a significant difference between a component having a mass of 801.4988 for 9.04 and a component having a mass of 767.4952 for 11.70. (T-test; p> 0.001).

(3) Furthermore, as a result of the mass fragmentation pattern, as shown in FIGS. 13 and 14, the standard marker of Korean red ginseng was represented by ginsenoside Rf (9.04 minutes, MW 801.4988), as shown in the following formula (3). The standard marker of Chinese red ginseng was identified as ginsenoside Rd (11.70 minutes, MW 767.4952) as shown in the following formula (4).

      [Formula 3] [Formula 4]

< Example  6> Standard for discriminating the age of red ginseng (4 years old, 6 years old) Marker  Establish

The following experiments were performed to establish markers for determining the origin of Korean 4 and 6 year old red ginseng.

Step 1: red ginseng Metabolic  extraction

Except for using the four-year-old domestic red ginseng and six-year-old domestic red ginseng prepared in Preparation Example 1 was carried out in the same manner as in Step 1 of Example 1.

Step 2: Liquid Chromatography-Mass Spectrometry LC - MS Analysis of Red Ginseng Extract Using

Except for using the metabolite of the red ginseng extract prepared in step 1 was carried out in the same manner as in step 2 of Example 1.

Results of total ion chromatogram of liquid chromatography-mass spectrometry (LC-MS) of the domestic four-year-old red ginseng extract and six-year-old red ginseng extract are shown in FIGS. 15 and 16.

Step 3: Convert Statistical Numbers to Processable Red Ginseng Standards Marker  Establish

The same procedure as in Example 3, except that the results obtained in step 2 were used.

result

(1) As a result of the score scatter plot of the orthogonal least squares analysis, as shown in FIG. 17, domestic four-year-old red ginseng and six-year-old red ginseng were divided into two groups according to lotus root.

(2) In addition, as a result of the S plot of the orthogonal least squares analysis, as shown in FIGS. 18 to 20, the components of four-year-old red ginseng and six-year-old red ginseng showed a significant difference in mass (t-test; p>). 0.001).

Of these, four-year-old red ginseng components are malonyl ginsenoside Rb1 (1195.6145 / 9.89 minutes) (Formula 5), malonyl ginsenoside Rc (10.30 minutes, MW 1165.6030) (Formula 6), malonyl ginsenoside Rb2 (10.75 Minutes, MW 1165.6036) (Formula 7), malonyl ginsenoside Rd (12.02 minutes, MW 853.4980) (Formula 8), and 6 years old red ginseng component (R) -ginsenoside Rh1 (10.48 minutes, MW 1277.8934) (Formula 9), Quinquenoside R1 (11.26 minutes, MW 1151.6239) (Formula 10), (R) -ginsenoside Rg3 (15.80 minutes, MW 749.4871) (Formula 11).

The four-year-old red ginseng components and six-year-old red ginseng components are shown in Table 2 below.

Chemical formula rescue Compound name




5

Malonyl Ginsenoside Rb1




6

Malonyl Ginsenoside Rc




7

Malonyl Ginsenoside Rb2




8

Malonyl Ginsenoside Rd



9

(R) -ginsenoside Rh1




10

Queen Quenoside R1



11

(R) -ginsenoside Rg3

Claims (21)

Marker for determining the location of the white ginseng represented by the formula (1) or 2 used for the determination of the production of domestic white ginseng and Chinese white ginseng.
[Formula 1]

(2)

According to claim 1, wherein the compound represented by the formula (1) is a marker for determining the origin of white ginseng, characterized in that the marker for determining the production of domestic white ginseng.
The method of claim 1, wherein the compound represented by the formula (2) is a marker for determining the origin of white ginseng, characterized in that the marker for determining the origin of Chinese ginseng.
Extracting metabolites from domestic white ginseng and Chinese white ginseng (step 1);
Analyzing the metabolite extracted in step 1 by liquid chromatography-mass spectrometry (LC-MS) (step 2); And
In step 2, the result analyzed by LC-MS is converted into a numerical value that can be statistically processed, and the data obtained by analyzing the converted value by the multivariate analysis method are detected. A method for establishing a white ginseng mountain determination marker according to claim 1, comprising the step (step 3) of establishing as a standard marker according to the mountain regions.
5. The method of claim 4, wherein the multivariate analysis method of step 3 is an orthogonal least square analysis method.
The method of claim 4, wherein the data obtained by analyzing the multivariate analysis of step 3 is a score scatter plot.
Extracting the metabolite from the test white ginseng (step 1);
Analyzing the metabolite extracted in step 1 by liquid chromatography-mass spectrometry (LC-MS) (step 2); And
Comparing the results of the LC-MS analysis obtained in step 2 with the marker for determining the mountain region of the formula (1) or claim 2 (step 3).
Marker for determining the location of the red ginseng represented by the formula (3) or (4) used to determine the production of domestic red ginseng and Chinese red ginseng.
(3)

[Chemical Formula 4]

The method of claim 8, wherein the compound represented by the formula (3) is a marker for determining the origin of red ginseng, characterized in that the marker for determining the origin of Korean red ginseng.
The method of claim 8, wherein the compound represented by the formula (4) is a marker for determining the origin of red ginseng, characterized in that the marker for determining the origin of Chinese red ginseng.
Extracting metabolites from domestic red ginseng and Chinese red ginseng (step 1);
Analyzing the metabolite extracted in step 1 by liquid chromatography-mass spectrometry (LC-MS) (step 2); And
In step 2, the result analyzed by LC-MS is converted into a numerical value that can be statistically processed, and the data obtained by analyzing the converted value by the multivariate analysis method are detected. A method of establishing a marker for determining the red ginseng mountain region of claim 8, comprising the step (step 3) of establishing as a standard marker according to the mountain region.
12. The method of claim 11, wherein the multivariate analysis method of step 3 is an orthogonal least square analysis method.
12. The method of claim 11, wherein the data obtained by analyzing the multivariate analysis of step 3 is a score scatter plot.
Extracting the metabolite from the red ginseng to be examined (step 1);
Analyzing the metabolite extracted in step 1 by liquid chromatography-mass spectrometry (LC-MS) (step 2); And
Comparing the results of the LC-MS analysis obtained in step 2 with the acid determination marker of the formula (3) or claim 8 (step 3) of the determination region of red ginseng.
Marker for determining the age of red ginseng represented by the following formula 5 to 11 used for the lotus root discrimination of 4 years old red ginseng and 6 years old red ginseng domestic.
[Chemical Formula 5]

[Chemical Formula 6]

(7)

[Chemical Formula 8]

[Chemical Formula 9]

[Formula 10]

(11)

16. The marker for determining age of red ginseng according to claim 15, wherein the compound represented by Chemical Formulas 5 to 8 is an age discriminating marker of 4 years old red ginseng.
The marker for determining the age of red ginseng according to claim 15, wherein the compound represented by the formulas (9) to (11) is a marker for determining the age of 6-year-old red ginseng.
Extracting metabolites from domestic 4 year old red ginseng and 6 year old red ginseng (step 1);
Analyzing the metabolite extracted in step 1 by liquid chromatography-mass spectrometry (LC-MS) (step 2); And
In step 2, the result analyzed by LC-MS is converted into a numerical value that can be statistically processed, and the data obtained by analyzing the converted value by the multivariate analysis method are detected. A method of establishing a marker for determining the age of red ginseng according to claim 15, comprising the step (step 3) of establishing as a standard marker according to the age of red ginseng.
19. The method of claim 18, wherein the multivariate analysis method of step 3 is an orthogonal least-squares analysis method.
19. The method of claim 18, wherein the data obtained by analyzing the multivariate analysis of step 3 is a score scatter plot.
Extracting the metabolite from the red ginseng to be examined (step 1);
Analyzing the metabolite extracted in step 1 by liquid chromatography-mass spectrometry (LC-MS) (step 2); And
Comparing the LC-MS analysis result obtained in step 2 with the age discriminating marker selected from the group consisting of compounds represented by the formulas 5 to 11 of claim 15 (step 3) age discrimination method.

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