KR20110130131A - Antimicrobial composition containing p. nepalensis and p. japonica extracts - Google Patents

Abstract

PURPOSE: A composition containing extract of Persicaria nepalensis or Persicaria japonica is provided to ensure excellent antibacterial activity without side effect. CONSTITUTION: A composition with antibacterial activity contains extract of Persicaria nepalensis or Persicaria japonica. The extract is prepared using low alcohol of C1-C6, hexane, dichloromethane, ethyl acetate, or water. A method for preparing the extract comprises: a step of refluxing extract of Persicaria nepalensis or Persicaria japonica with one of the solvents at 0-30 °C for 12-36 hours to prepare extract; a step of filtering the extract; a step of vacuum-drying the extract at 20-60 °C for 6-12 hours; and a step of freeze-drying the dried extract at -40 to 20 °C for 24-48 hours. A functional food composition with antibacterial activity contains the extract as an active ingredient.

Description

Antimicrobial Composition Containing Extracts from Wildflowers and White Flowers {Antimicrobial composition containing P. nepalensis and P. japonica extracts}

The present invention relates to a composition having an antimicrobial activity containing an extract of wild flowers and white flowers.

Recently, as a well-being culture has been established in our society, research trends have been made to exclude chemical components and use plant-derived ingredients in cosmetics. As a result, the development of cosmetics using a variety of natural materials has been developed (Shim EB, The study of preservation system in cosmetics using botanical antimicrobial, Kor . J. Aca . Indu . Soc . , 9 (5): 1344-1348, 2008). Since these cosmetics contain a large amount of water, it is easy to cause contamination by bacteria or fungi, and products contaminated with microorganisms not only exhibit deterioration of odor, color change, viscosity change, texture change, mold occurrence, etc. It causes serious eye diseases such as corneal ulcers, and it is harmful to the human body. Therefore, in order to prevent the decay caused by microorganisms and to store the product for a long time, food, cosmetics, pharmaceuticals, etc. are essentially used as a preservative. Among them, cosmetics have a long shelf life, are highly likely to come into contact with microorganisms in their use and storage methods, contain various carbon and nitrogen sources, and contain moisture that is essential for microbial growth. There are more needs and problems with treatment (Ok KK, et. al . , New cosmetical, Donghwa Publishing Company, 1997).

The types of preservatives used in cosmetics are very diverse, and as the production of cosmetics increases, preservatives are more diverse and the amount of their preservation is increasing, but most of them are developed as chemical preservatives. The function of chemical preservatives is to impart stability to the product by inhibiting the growth of microorganisms. Typical examples include ester oxidation stabilizers such as dibutyl hydroxytoluene (BHT), butyl hydroxy anisol (BHA) and gallic acid, parabens (paraoxybenzoate), imidazolidinyl urea, di Preservatives, such as salts of hydroacetic acid, are used. In particular, parabens are effective against fungi including filamentous fungi and yeasts, have relatively low toxicity, and have a broad spectrum of fungi against bacteria (D. Steinberg, et al. , The effect of parabens in a mouthwash). and incorporated into a sustained release varnish on salivary bacteria, J. Dentistry , 27 (2): 101, 1999). However, as with most chemical preservatives, it is reported to have a tendency to cause allergic contact dermatitis (SMCooper and S. Shaw, Allergic contact dermatitis from parabens in a tar shampoo, Contact Dermatitis , 39 (3): 140, 1998). In addition, new problems such as potential as environmental hormones, causing resistant bacteria, acute and chronic toxicity due to continuous accumulation in the body, and mutagenesis are emerging (DH Shin, Food science and industry, 23 (4): 68, 1990). .

Therefore, in order to solve the problem of safety and skin irritation in cosmetics, a condition derived from natural preservatives and fungicides is required. As a study on natural antimicrobial substances, many natural antimicrobial substances have been reported from foods such as flavors, milk and fish, essential oils and herbal medicines, alkaloids, flavonoids, phytoalexin, antibacterial peptides, organic acids And antimicrobial properties such as fatty acids have been reported. However, due to the problems of color, odor, stability, formulation, and narrow antibacterial spectrum, which are limitations as natural substances, most of them are not practically used. Among them, some of the natural antimicrobial derivatives are Hinokitiol, a magnolia extract, and magnolia extract. Magnonol and grapefruit seed extract DF-100 have been developed, but the antimicrobial activity of DF-100 is due to the synthetic preservatives (benzethonium chloride) contained in it. There is a need for more advanced natural antimicrobial research.

Accordingly, the present inventors have studied to overcome the problems of the prior art as described above, by solving the above-mentioned problems by finding a composition containing the extract of natural or wild flowers and natural extraction method of the extract, As well as finding a natural herbal extract having a wide range of antimicrobial spectrum, and found that when used as a cosmetic composition and functional food composition it was found that the excellent antimicrobial effect can be obtained to complete the present invention.

Accordingly, an object of the present invention is to provide a composition comprising an extract extracted from a mountainside or white flower field having an antimicrobial activity and a method for producing the extract.

In addition, there is no side effect to the human body, as a natural herbal material that can supplement the side effects, such as parabens, which is a cosmetic preservative, to provide a cosmetic composition and a functional food composition comprising the extract.

The present invention is an antimicrobial activity characterized in that it comprises an extract extracted from acid or white flower using one or more substances selected from the group consisting of C ₁ -C ₆ lower alcohol, hexane, dichloromethane, ethyl acetate and water as a solvent Eggplant relates to a composition and a method of preparing the extract.

The present invention also relates to a cosmetic composition and a functional food composition having an antimicrobial activity, characterized in that it contains the extract as an active ingredient.

The extract extracted from Sanchi or white flower according to the present invention has a high antimicrobial activity, it can be used as a composition of various products that require antimicrobial activity. In particular, it can solve allergic contact dermatitis, which is a problem with cosmetics including conventional chemical preservatives. In addition, it has a broad antimicrobial spectrum and has the advantage of being excellent in safety and can be used as a natural antimicrobial agent. When the extract of the present invention is applied to the cosmetic and food fields, there is an advantage that it can contribute to the enhancement of immunity and health in the body.

Figure 1 is a flow chart illustrating a process for separating the extract at several times using a 70% ethanol aqueous solution.
Figure 2 is a flow chart showing the organic solvent fractionation of white flower shift and mountain shift.
Figure 3 is a photograph showing the antimicrobial activity evaluation of the organic solvent fractions from the white flower shift (top) and acid shift (bottom) extract.
Figure 4 is a photograph showing the results of the minimum inhibition concentration (MIC) test for dichloromethane fractionation layer (upper) and ethyl acetate fractionation layer (lower) of white flowers.

The present invention is an antimicrobial activity characterized in that it comprises an extract extracted from acid or white flower using one or more substances selected from the group consisting of C ₁ -C ₆ lower alcohol, hexane, dichloromethane, ethyl acetate and water as a solvent Eggplant relates to a composition.

Hereinafter, the present invention will be described in detail.

In the present invention, 'Persicaria nepalensis' is a perennial herb, and the height is about 30 cm, and in August, the reddish flowers gather at the tip of the leaf axilla and the branch, and the fruit bears the asteraceae. . It grows in wetlands of fields or valleys, and is distributed in Korea, China, and Malaysia.

'Persicaria japonica' in the present invention is a perennial herb of perilla, the height is about 60 ~ 100 cm, the leaves are offset and lanceolate. It blooms in July-August and forms aquatic fruit in September, and the fruit is characterized by oval ovate, triangular or lenticular gloss. It grows in fields or waterside, and is distributed in sub-central Korea, Japan, and China.

In Korea, they grow wild in Baekunsan, Jeonsi-si, Taehwasan, and Yeonwol-gun Yeondang-ri.

The extract of the acidic or white flower of the present invention can be prepared using the following method, but is not limited thereto.

First, prepare domestic wild flowers and white flowers that have been washed, dried and ground. The mixture was refluxed for 12 to 36 hours at 0 to 30 ° C. using a solvent, filtered and vacuum dried for 6 to 12 hours at 20 to 60 ° C., and then freeze-dried at -40 to 20 ° C. for 24 to 48 hours, respectively. The extract can be prepared alone.

In the preparation of the extract, the solvent may be one or more substances selected from the group consisting of C ₁ -C ₆ lower alcohol, hexane, dichloromethane, ethyl acetate and water. It is preferable to use 40 to 80% ethanol, and more preferably to use 50 to 70% ethanol can separate the extract with high antimicrobial activity. Most preferably 70% ethanol is used. In case of white flower, the antibacterial activity of the extract was better when 50% ~ 100% ethanol solution was used. There is a characteristic.

The extract of the mountain or white flower according to the present invention can be used in the composition. The extract of the present invention is preferably included in 0.001 to 99.99% by weight relative to the total weight of the composition, more preferably contained in 0.1 to 50.0% by weight is good in terms of effects and stability.

In addition, in the present invention, by going through an additional process, it is possible to separate the organic solvent fraction layer containing a large amount of the active ingredient from the extract, for this purpose, the extract separated by the preparation method as a dichloromethane, ethyl acetate or butanol solvent One more fractionation process. The fractionation process may generally use without limitation methods used in the art.

For example, the organic solvent layer containing a large amount of the active ingredient is separated by subjecting the extract extracted with 40 to 80% ethanol as a solvent to the dichloromethane, ethyl acetate or butanol solvent once more. Can be. The extract of white flower shows the best antimicrobial activity when it is fractionated with dichloromethane and the extract of acidic leaves is fractionated with ethyl acetate or butanol.

The composition containing the extract of the mountain or white flower as an active ingredient has antibacterial activity can be used for the prevention and treatment of skin diseases. As the skin disease, acne, atopy and the like are preferable. The composition comprising the extract of the mountain or white flower is Propionibacterium acnes ( Acterium acne) and aerobic Gram-positive strains Staphylococcus aureus , Bacillus ( Bacillus) subtilis ) or Candida albicans . In addition, the composition containing the extract of the acid or white flower as an active ingredient can be used for skin aging, skin fine wrinkle improvement and skin whitening.

The composition according to the present invention may include, in addition to the active ingredient, a pharmaceutically acceptable carrier, excipient or diluent.

The compositions of the present invention may be in various oral or parenteral formulations. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid form preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, which form at least one excipient such as starch, calcium carbonate, sucrose or lactose (at least one compound). lactose) and gelatin. In addition to simple excipients, lubricants such as magnesium stearate, talc and the like are also used. Liquid preparations for oral administration include suspensions, solutions, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. have.

The composition of the present invention may be administered orally or parenterally, and may be used as an external skin preparation or injection such as a dressing agent, a patch, a bandage, an ointment, and the like during parenteral administration.

The dosage of the composition of the present invention varies depending on the weight, age, sex, health status, diet, time of administration, administration method, excretion rate and severity of the disease of the patient, the daily dosage is the amount of gelatin degrading product 0.1 to 100 mg / kg, preferably 30 to 80 mg / kg, more preferably 50 to 60 mg / kg, and may be administered 1 to 6 times per day.

In addition, it is also possible to use in combination with existing skin disease prevention and treatment or skin whitening agent.

In addition, the present invention includes a functional food containing an extract of wild flowers or white flowers as an active ingredient.

Functional foods according to the present invention, for example, chewing gum, caramel products, candy, ice cream, confectionery, such as various food products, soft drinks, mineral water, alcoholic beverages, beverage products such as vitamins and minerals Can be.

The extracts of wild flowers or wild flowers of the present invention may be added as they are, or may be used together with other foods or food ingredients, and may be appropriately used according to conventional methods. The blending amount of the active ingredient may be appropriately determined depending on the purpose of use (prevention, health or hygiene).

The functional food of the present invention may contain various flavors, natural carbohydrates, and the like as additional ingredients. The above-mentioned natural carbohydrates are glucose, monosaccharides such as fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is preferably selected in the range of 0.01 to 0.04 parts by weight, preferably about 0.02 to 0.03 parts by weight, per 100 parts by weight of the health food of the present invention.

In addition to the above, the functional food of the present invention includes various nutrients, vitamins, electrolytes, flavors, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols. And carbonation agents used in carbonated beverages. In addition, the functional food of the present invention may contain fruit flesh for the production of natural fruit juice, fruit juice drink and vegetable drink. These components can be used independently or in combination.

On the other hand, the present invention includes a cosmetic composition containing an extract of wild flowers or white flowers as an active ingredient. The extract containing cosmetics of the mountain or white flower can be prepared in liquid or solid form using bases, adjuvants and additives commonly used in the cosmetic field. Cosmetics in liquid or solid form may include, but are not limited to, for example, cosmetics, creams, lotions, baths and the like. Bases, auxiliaries and additives commonly used in the cosmetic field are not particularly limited and may include, for example, water, alcohols, propylene glycol, stearic acid, glycerol, cetyl alcohol and liquid paraffin.

Cosmetics according to the present invention can be very useful for maintaining stability, etc. without using an artificial preservative because of excellent antibacterial action.

As described above, the extract and the composition using the same according to the present invention have a broad antimicrobial spectrum and ensure the safety, and thus can be widely used as a natural preservative.

Although this invention is demonstrated in more detail based on the following Example, this invention is not limited by the following Example.

< Example  1> Come on  Preparation of White Flower Extract

In order to determine the extraction yield according to the ethanol concentration and to test the antimicrobial activity afterwards, 500 kg of Ginseng and white flowers were collected from Baegunsan, Jecheon-si, Chungbuk. The water was first washed with water, dried until completely dried and then ground. After refluxing at room temperature (20 ° C) for 24 hours with an aqueous ethanol solution of concentration 0, 10, 30, 50, 70 and 100% (V / V), filtered through filter paper and vacuum-dried at 40 ° C for 10 hours- Each extract was prepared by lyophilization at 20 ° C. for 36 hours. The yield of the extract according to the concentration of the ethanol aqueous solution is shown in Table 1 below.

White flowers Mountainside  Extraction and yield by ethanol concentration ethanol (%) Yield (%) White flowers Mountain 0 12 15 10 12 18 30 14 19 50 13 20 70 14 17 100 6 9

< Example  2> According to the difference in concentration of organic solvent Come on  Evaluation of Antimicrobial Activity of Extracts from White Flowers and Confirmation of Optimal Condition for Extraction

In order to examine the antimicrobial effect of the extract according to the difference in concentration of the organic solvent used, the antimicrobial activity was investigated using each extract prepared in Example 1.

In order to investigate the antimicrobial activity of acne causing bacteria Propionibacterium sludge tumefaciens arc Ness (Propionibacterium acnes) and Staphylococcus aureus in the aerobic gram-positive strain (Staphylococcus aureus ), Bacillus subtilis or Candida albicans were used to examine the antimicrobial activity of extracts of white flowers and wild flowers. The medium for propionibacterium acnes is GAM broth, Staphylococcus aureus and Candida albicans from Nissui (Japan). Luria-Bertani (LB) broth of Difco (France), Bacillus bacterium medium was used Nutrient broth, agar plate (Agar plate) of Difco (France) was used.

The antimicrobial activity was evaluated using a paper disc measuring the diameter of the inhibitory ring showing the antimicrobial activity. The size of the inhibitory ring was measured and expressed as ± when 1 to 5 mm size, + when 5 to 15 mm size, and ++ when 15 to 20 mm size.

The strain was used after inoculating 1% in each of the corresponding media and incubating for 24 hours at 37 ℃. Each of the extracts prepared in Example 1 was prepared in 10 mg / mL in the strain-containing medium, followed by 10 uL dropwise incubation at 37 ° C. for 24 hours. The measured antimicrobial effect is shown in Tables 2 and 3 below.

Antimicrobial Effect of Ethanol Concentrate Extract on White Flower Extraction Concentration (%) P. acnes Can . albicans Sta . aureus B. subtilis 0 - - - - 10 - - - - 30 - - - - 50 + + + + 70 ++ ++ ++ + 100 + ++ + +

Mountain  Antimicrobial Effects on Extracts by Ethanol Concentration Extraction Concentration (%) P. acnes Can . albicans Sta . aureus B. subtilis 0 - - - - 10 - - - - 30 - - - - 50 ± ± ± ± 70 + + + + 100 + ++ + +

In light of the results of Tables 2 and 3 above, the extract of the ethanol aqueous solution of 50% to 100% in the case of the white flower is the most excellent antibacterial activity, while the ethanol aqueous solution of 70% to 100% in the case of acid When used, the extract had the best antibacterial activity. Therefore, in the case of the general separation of the extract, it was confirmed that the 70% ethanol aqueous solution is the condition that can be most optimal separation of the excellent antibacterial activity.

< Example  3> Come on  Organic solvent having the active ingredient of white flower extract Fraction  detach

In order to fractionate a portion having an active ingredient having antimicrobial activity among the extracts separated by Example 1, fractions were sequentially performed using various organic solvents.

The extract extracted using the 70% ethanol aqueous solution excellent in the antibacterial activity of Example 1 was used, which was sequentially fractionated using organic solvents hexane, dichloromethane, ethyl acetate and butanol.

First, 50 g of 70% ethanol extract was dissolved in 1 L of distilled water, and then 1 L of hexane was added to the hexane layer and the water layer using hexane continuously in the same volume as the water layer until the hexane layer became completely white. After separation, 1 L of dichloromethane was added to the obtained water layer, and the dichloromethane layer and the water layer were separated. Thereafter, 1 L of ethyl acetate was added in the same manner as above to separate the ethyl acetate and the water layer. Then, 1 L of butanol was added to the separated water layer, followed by separation of the butanol and water layers. Each fraction was vacuum lyophilized under the same conditions as in Example 1. The yield for each organic solvent fraction is shown in Table 4 below.

White flowers Mountain  From 70% ethanol extract
Organic solvent In fractions  Yield yield(%) White flowers Mountain Hexane layer 9.4 9.5 Dichloromethane layer 7.1 7.4 Ethyl acetate layer 7.9 24.5 Butanol layer 19.6 15.3 Water layer 48.0 40.7

< Example  4> Come on  Organic solvent having the active ingredient of white flower extract Fraction  Antimicrobial Activity Assessment

The organic solvent fraction was carried out in the same manner as in Example 3 using the white flower extract and the acid extract extracted in 70% ethanol extraction conditions in Example 1, in this embodiment to the fraction containing the active ingredient obtained as described above Antimicrobial evaluation was performed. Antimicrobial activity evaluation was evaluated under the same conditions as in Example 2.

The resultant antimicrobial activity of each fraction was measured and shown in Tables 5, 6 and FIG. 3.

Organic solvent from white flower extract In fractions  Antimicrobial evaluation P. acnes
(SMB-2) Can . albicans
(SMB-3) Sta . aureus
(SMB-4) B. subtilis
(SMB-7) Hexane layer - - + - Dichloromethane layer ++ ++ ++ + Ethyl acetate layer + + + + Butanol layer + + + + Water layer - - - -

Mountain  Organic Solvents from Extracts In fractions  Antimicrobial evaluation P. acnes
(SMB-2) Can . albicans
(SMB-3) Sta . aureus
(SMB-4) B. subtilis
(SMB-7) Hexane layer - - - - Dichloromethane layer + ± + + Ethyl acetate layer ++ ± + + Butanol layer ± ++ + ++ Water layer - - - -

As can be seen in Table 5 and Table 6, the substance having the antimicrobial activity of the white flower extract is a non-polar substance, while the substance having the antimicrobial activity of the extract is acidic substance was confirmed to be a polar substance.

As can be seen from the above results, the white flower shift extract was found to have the best antibacterial activity in the dichloromethane layer and the extract of acid shift in ethyl acetate or butanol layer.

< Example  5> Minimum Inhibitory Concentration ( Minimum Inhibitory Concentration  : MIC ) exam

According to the results of Example 4, the minimum inhibitory concentration test was carried out using the dichloromethane layer and the ethyl acetate layer of the extract, which was excellent in antibacterial activity. This value refers to the concentration at which the strain does not grow at the minimum inhibitory concentration of the ethyl acetate fraction layer.

In order to determine the antimicrobial effect by using the fraction was measured the minimum inhibitory concentration (MIC) using a solid medium dilution method. The minimum inhibitory concentration value was set to the minimum inhibitory concentration at which the test strains did not grow on the agar medium after each sample was serially diluted to twice the concentration (20 mg / mL to 0.625 mg / mL) and treated in agar medium.

First, the strains of Tables 7 and 8, which were cultured through the same process as in Example 2, were inoculated with 1% of each agar medium, followed by incubation at 37 ° C. for 24 hours. After the initial concentration of 20, 10, 5, 2.5, 1.25 and 0.625 mg / mL in the medium containing the strain was added to each 10 uL it was confirmed that the strains do not grow in agar medium.

The results were measured and shown in Tables 7 and 8 below.

White flowers Dichloromethane In the fractionation layer  Minimum inhibitory concentration ( MIC ) exam Concentration (mg / mL) Can . albicans
(SMB-3) Sta . aureus
(SMB-4) B. subtilis
(SMB-7) 20 ++ ++ ++ 10 + + + 5 ± ± ± 2.5 - - - 1.25 - - - 0.625 - - -

Mountain  Ethyl acetate In the fractionation layer  Minimum inhibitory concentration ( MIC ) exam Concentration (mg / mL) Can . albicans
(SMB-3) Sta . aureus
(SMB-4) B. subtilis
(SMB-7) 20 ++ ++ ++ 10 + + + 5 ± ± ± 2.5 - - - 1.25 - - - 0.625 - - -

As a result of Tables 7, 8 and 4, it can be seen that the minimum inhibitory concentration at which the strains do not grow in agar medium is 5 mg / mL.

< Formulation example  1> Preparation of Ointment

The ointment was prepared in the following composition using the acid-extracted extract obtained using 70% ethanol in Example 1 as an active ingredient.

[Furtherance]

Acid extract 1.0% by weight, beeswax 10% by weight, polysorbate-60 5.0% by weight, Fiji-60 hardened castor oil 2.0% by weight, sorbitan sesquioleate 0.5% by weight, petrolatum 5.0% by weight, liquid paraffin 10.0% by weight %.

< Formulation example  2> Patchwork  Produce

The patch was prepared in the following composition using the acid-extracted extract obtained using 70% ethanol in Example 1 as an active ingredient.

[Furtherance]

Acid extract 1.2 wt%, hexylene glycol 20.0 wt%, dietylamine 0.7 wt%, polyacrylic acid 1.0 wt%, sodium sulfite 0.1 wt%, polyoxyethylene lauryl ether 1.0 wt%, polyhydroxyethylene cetyl stearyl 1.0 weight percent ether, 2.5 weight percent viscous paraffin oil, 2.5 weight percent caprylic acid ester / capric acid ester, 3.0 weight percent polyethylene glycol-400, 100 weight percent deionized water.

< Formulation example  3> Preparation of functional food

< Production of Drinks>

100 mg extract

Citric acid 1000 mg

100 g oligosaccharides

Plum concentrate 2 g

1 g of taurine

Add 900 ml of purified water

After mixing the above components according to the conventional healthy beverage manufacturing method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and then refrigerated and stored Used to prepare beverage compositions of the invention.

Although the composition ratio is a composition that is relatively suitable for the preferred beverage in a preferred embodiment, the compounding ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and usage.

< Manufacturing example  4> Cosmetic  Produce

Essence was prepared in the following composition using the acid-extracted extract obtained using 70% ethanol in Example 1 as an active ingredient.

[Furtherance]

Active ingredient 10.0 mg, glycerin 3.0 mg, EDTA 0.05 mg, benzophenone-9 0.04 mg, carboxyvinyl polymer 0.2 mg, triethanolamine 0.18 mg, oxidotocese-25 0.6 mg, glyceryl monostearate 1.0 mg, preservative 0.01 mg, fragrance 0.01 mg, remaining amount of purified water

< Example  6> Mountain  With extract Cosmetics  Comparative experiment of antimicrobial effect between composition and cosmetics containing preservative

The antimicrobial activity was carried out using the strain cultured under the same conditions as in Example 2 using the cosmetic product containing the acid-extracted extract composed of the composition of Preparation Example 4 and the existing cosmetics (NOV W sensor, NOEVIR, Inc.). The same result as 7 was confirmed.

P. acnes
(SMB-2) Can . albicans
(SMB-3) Sta . aureus
(SMB-4) B. subtilis
(SMB-7) Manufacturing example  4 composition ++ + + + Original cosmetic ± ± + +

As a result, the acne-causing bacteria It was confirmed that the antibacterial activity against Propionibacterium acnes was the best.

Claims (7)

C ₁ -C _{6 A} lower alcohol, hexane, dichloromethane, ethyl acetate and water using an at least one substance selected from the group consisting of a solvent extracted from an acid or white flower composition having an antimicrobial activity, characterized in that it comprises.
According to claim 1, wherein the antibacterial effect is Propionibacterium ( Propionibacterium) acnes ), Staphylococcus aureus ), Bacillus subtilis ) or Candida albicans antimicrobial effect.
(a) reflux extraction for 12 to 36 hours at 0 to 30 ° C. using one or more substances selected from the group consisting of C ₁ -C ₆ lower alcohol, hexane, dichloromethane, ethyl acetate and water Making an extract;
(b) filtering the extract;
(c) vacuum drying the filtered extracts at 20-60 ° C. for 6-12 hours; And
(d) freeze drying the dried extract for 24 to 48 hours at -40 to 20 ℃
Method of producing an extract of wild or white flowers with antibacterial activity, characterized in that it comprises a.
[4] The method of claim 3, wherein 40 to 80% of ethanol is used as the solvent of step (a).
[5] The method according to claim 4, further comprising the step of obtaining a dichloromethane, ethyl acetate or butanol fraction of the extract of acid or white flower.
Functional food composition having an antimicrobial activity, characterized in that it contains an extract according to claim 1 as an active ingredient.
Cosmetic composition having an antimicrobial activity, characterized in that it contains an extract according to claim 1 as an active ingredient.

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