KR20130078526A - Composition for inhibiting oral bacteria comprising the extract of ixeris dentata - Google Patents

Abstract

PURPOSE: A composition containing an Ixeris dentata NaKai extract as an active ingredient for suppressing oral bacteria is provided to suppress the growth of Streptococcus mutans, generation of organic acid, and formation of a Streptococcus mutans biofilm, thereby preventing, alleviating, and treating dental diseases without side effects. CONSTITUTION: A composition for suppressing oral bacteria contains an Ixeris dentata NaKai extract as an active ingredient. The extract suppresses the growth of Streptococcus mutans, generation of organic acid by Streptococcus mutans, and formation of a Streptococcus mutans biofilm. A preventive and therapeutic agent for dental diseases for suppressing oral bacteria contains the extract as an active ingredient. The preventive and therapeutic agent is formulated in a unit dose form by adding a pharmaceutically acceptable carrier, excipient, or diluents. The preventive and therapeutic agent is manufactured in the form selected from a group consisted of an oral cleanser, a toothpaste, an oral spray, and a denture cleaner.

Description

Composition for Inhibiting Oral Bacteria Containing Mugwort Extract as Active Ingredient {Composition for Inhibiting Oral Bacteria comprising the extract of Ixeris dentata}

The present invention inhibits the growth of Streptococcus mutans, inhibits the production of organic acids by Streptococcus mutans, and also suppresses the production of Streptococcus mutans biofilm in dental diseases caused by crust and oral bacteria. And to compositions for improving and treating.

The composition for the prevention, improvement and treatment of the dental disease of the present invention has a technical feature to include the moth extract as an active ingredient.

The main cause of dental diseases such as tooth decay and periodontal disease is microorganisms in the oral cavity. Oral microorganisms form a dental plaque, a kind of biofilm, which forms dental caries and periodontitis. Cause.

A representative oral bacterium that forms the plaque is Streptococcus mutans . Streptococcus mutans adhere to the surface of the tooth to form colonies, form salivary bacterial membrane substrates using saliva proteins, and stabilize the structure of the bacterial bacterial membranes by synthesizing water-insoluble glucans using sugars present in the oral cavity.

In addition, Streptococcus mutans metabolizes energy using carbohydrates, glucose, and fructose in oral foods, and demineralizes tooth enamel by releasing organic acids as extra-products into the cells.

Through this process, bacteria and its metabolites in the periodontal bacterial membranes cause an inflammatory response of the periodontal tissue, and also cause dental caries due to local stagnation of the organic acid to continue demineralization.

On the other hand, in order to combat the causative agent of the formation of dental plaque, various chemicals including fluorine compounds, various devices and materials, and automatic brushing devices have been developed and introduced.

However, chemicals are effective in preventing tooth decay, but there are limitations such as vomiting, diarrhea and antibiotic resistance.

Therefore, the development of more effective, less side effects and stable dental plaque suppression method is required.

On the other hand, Ixeris dentata Nakai) is an annual or biennial herb belonging to the compositae, distributed mainly in Korea, Japan, and China, and grows in mountains, fields, and fields.

Its height reaches 20 ~ 50cm and branches are split from the upper part. The outpost contains cinnaroside, a kind of nucleenol and flavonoids, which has excellent blood sugar lowering and lipid lowering effects, and is excellent in anti-cancer 'aliphatic', anti-aging and antioxidant It is rich in ingredients such as the functional "cinaroside". It also contains components such as triterpenoids, which are immunostimulating substances, and sesquiterpene glycosides known as anticancer active ingredients.

In oriental medicine, it is also called chaemin (황 菜), yellow and green (黄瓜 彩). In spring, all parts of the plant, including roots, are used for edible, medicinal and ornamental purposes.

On the other hand, a paper published in relation to the moths, “Analysis of the roots of the moths and the cell viability and DPPH radical scavenging activity of extracts” (Hong Seul-Ki et al., 3), Journal of the Korean Nutrition Society, 2010, Vol.43 No.2 The results of examining the survival rate and antioxidant activity of kidney cell line (vero cell) and hepatocyte cell line (chang cell) of the extract of white moth extract are disclosed.

In addition, "Physical activity of fresh moth extract" (Myung-Jo Kim et al., Korean Society of Food and Nutrition, Vol. 31, No. 5, 2002.10, page (s): 924-930) showed that moth powder had antimutagenic and cancer cell growth inhibitory effects. Has been disclosed.

In addition, "Antibacterial Activity Test against Staphylococcus sp. From Organic Solvent Extracts from Edible Plants" (Chul-Gyu Ha et al. 2, Korean Society for Biotechnology and Bioengineering, Vol. 15 No.3, 2000., 226-231) It has been shown to show high antimicrobial activity against coli and B. subtilis.

In summary, conventionally, there was no attempt to prevent or improve dental caries and periodontal disease by suppressing oral bacteria of the moth extract, and it is urgent to study this.

An object of the present invention is to provide a composition for preventing, ameliorating and treating dental diseases by continuously taking without causing side effects.

SUMMARY OF THE INVENTION An object of the present invention is to suppress dental disease by inhibiting growth of Streptococcus mutans, inhibiting organic acid production by Streptococcus mutans, and suppressing the production of Streptococcus mutans biofilm in dental diseases caused by oral bacteria. It is to provide a composition for preventing, improving and treating.

The present invention is to solve the technical problem by providing a therapeutic agent for preventing and treating dental diseases formulated in a pharmaceutical unit dosage form by adding a pharmaceutically acceptable carrier, excipient or diluent, etc. do.

The carrier, excipient, and diluent may include tods, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, undetermined. Vaginal cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

The pharmaceutical dosage forms may also be used in the form of pharmaceutically acceptable salts, and may be used alone or in combination with other pharmaceutically active compounds as well as in a suitable set.

In addition, when formulating the active ingredient containing the moth extract may be prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, surfactants, etc. commonly used.

In addition, each of the pharmaceutical dosage forms may be formulated in the form of powders, granules, tablets, capsules, oral preparations such as suspensions, emulsions, syrups, aerosols, suppositories, and sterilized injection solutions according to conventional methods .

In the solid preparation for oral administration, at least one excipient such as starch may be prepared by mixing calcium carbonate, sucrose, lactose, gelatin or the like in the extract. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used.

The preparation for parenteral administration may include a sterilized aqueous solution, a non-aqueous solvent, a suspension, an emulsion, a freeze-dried preparation, and a suppository.

Examples of the non-aqueous solvent and the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like.

Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The preferred dosage of the active ingredient according to the present invention may be appropriately selected by those skilled in the art depending on the condition and the weight of the patient, the degree of disease, age, sex, drug form, administration route and period.

The extract of the present invention can be administered to mammals such as rats, mice, livestock, humans and the like in various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, muscular, subcutaneous injection.

The present invention can be prepared according to a known method by adding all or a part of the components of the oral composition commonly used in the active ingredient containing the moth extract.

For example, mouthwashes can be prepared by adding abrasives, fluorides as medicinal agents, binders, foaming agents, flavorings, sweeteners, buffers, alcohols and other ingredients.In the case of toothpaste, fluorides as abrasives and medicinal agents , Can be prepared by adding a binder, foaming agent, flavoring agent, sweetening agent, buffer, etc. In the case of gum can be prepared by adding a gum base, sugar powder, glucose, starch hydrolyzate, glycerin, flavoring, etc. In general, sugar, starch syrup, sorbitol, emulsifier, shortening fat, flavoring, coloring, sweetener and the like can be prepared, and in the case of jelly, ordinary sugar, gelling agent, purified water, citric acid, liquid fructose, etc. In the case of chocolate, it can be prepared by adding cocoa butter or the like as sugar, cocoa mass, powdered milk, or emulsifier.

The present invention is to solve the technical problem by providing a dietary supplement composition for the prevention and improvement of skin diseases by adding a food supplement additive to the active ingredient containing the moth extract.

Examples of foods to which the extract can be added include various foods, beverages, gums, tea, vitamin complexes, and health functional foods.

It can also be added to foods or beverages for the purpose of preventing and improving skin aging.

At this time, the amount of the extract in the food or beverage may be 0.01 to 20% by weight of the total food, and the health beverage composition may be added in a proportion of 0.02 to 5 g, preferably 0.3 to 1 g, have.

The health functional beverage composition of the present invention is not particularly limited to the components other than those containing the above extract, and may contain various flavors or natural carbohydrates as additional components such as ordinary beverages.

Examples of the above-mentioned natural carbohydrates are monosaccharides such as glucose, fructose; Disaccharides such as maltose, sucrose and the like and polysaccharides such as dextrins, cyclodextrins and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. In addition to the above, natural flavoring agents (tau martin, stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavorings.

The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above, the active ingredient of the present invention can be used as a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and thickening agents (cheese, chocolate etc.), pectic acid and its salts, Preservatives, colloidal thickeners, pH adjusting agents, stabilizers, antiseptics, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like.

In addition, the active ingredient of the present invention may contain flesh for the production of natural fruit juice and fruit juice drinks and vegetable drinks.

These components may be used independently or in combination. The proportion of such additives is not so critical, but is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the extract of the present invention.

The composition containing the present invention moth extract as an active ingredient, by taking continuously without bringing side effects, the effect of preventing, improving and treating dental diseases is remarkable.

The composition containing the present invention extract as an active ingredient, the effect of inhibiting the growth of Streptococcus mutans is remarkable.

The composition containing the present invention extract as an active ingredient, the effect of inhibiting the generation of organic acids by Streptococcus mutans is remarkable.

The composition containing the present invention extract as an active ingredient, the effect of suppressing the production of Streptococcus mutans biofilm is remarkable.

1 is a view showing the growth inhibition test results of Streptococcus mutans according to Experimental Example 1.
2 is a view showing the results of the organic acid production inhibition test by Streptococcus mutans according to Experimental Example 2.
3 is a view showing the experimental results of the suppression of streptococcus mutans biofilm production according to Experimental Example 3.

The terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms and the inventor may properly define the concept of the term in order to best describe its invention It should be construed as meaning and concept consistent with the technical idea of the present invention.

Therefore, the embodiments described in the present specification and the configurations shown in the drawings are merely the most preferred embodiments of the present invention and are not intended to represent all of the technical ideas of the present invention. Therefore, various equivalents It should be understood that water and variations may be present.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Example  1. Preparation of nirvana extract

In order to prepare the extract extract for use in this experiment, the extract was soaked in ethanol for 2 weeks in advance and filtered.

The filtered material was concentrated under reduced pressure at room temperature for 2 days, and 70 g of 6% of 70% ethanol was added to 100 g of crust to make a concentrated solution of 7.2 g. The yield was 7.2%.

By repeating the above process, to obtain the extract extract, was used while storing the sample in the freezer.

Reference Example  1. Experimental preparation

1-1. Strain and Culture Preparation

The strains used in this experiment Streptococcus mutans (S. mutans) as ATCC 25175 Brain heart infusion (BHI, Difco, USA) to the liquid medium 1-2 and inoculated in the same medium and then cultured in a primary passage of the thermostat 37 ℃ 24 Time incubation.

1-2. Statistical processing

Student t- test and one-way ANOVA test were used for the significance test. P <0.05 was the limit of significance and the experimental results were expressed as mean ± SE.

Experimental Example  1. Crow Extract Streptococcus Mutans  Inhibitory effect on growth

1-1. Experimental Method

BHI ethanol extract according to Example 1 was added to a BHI liquid medium containing 1% glucose, and the bacteria were inoculated to 1 × ^{10 8} CFU / ml.

After 24 hours of incubation at 37 ^° C., the absorbance was measured at 550 nm using an ELISA reader (Molecular Devices Co., CF., USA) based on BHI liquid medium.

The control group was administered without adding the extract.

1-2. Streptococcus Mutans  Growth inhibitory effect

In order to observe the antimicrobial activity of Streptococcus mutans of S. mutans extract according to Example 1, extracts of S. musicus for each solvent were added to BHI liquid medium at concentrations of 0.25, 0.5, 1, 2, and 4 mg / ml. After the inoculation of Streptococcus mutans, the cells were incubated for 24 hours in a 37-degree incubator, and the effects on growth inhibition of Streptococcus mutans were observed.

1 shows the results of measuring absorbance and the relative growth rate based on the control group.

Referring to FIG. 1, it can be seen that the crust inhibits the growth of S. mutans in a concentration-dependent manner.

Experimental Example  2. Crumple Extract Streptococcus To Mutans  Effect of inhibiting organic acid production

2-1. Experimental Method

BHI ethanol extract according to Example 1 was added to a BHI liquid medium containing 1% glucose, and the bacteria were inoculated to 1 × ^{10 8} CFU / ml.

After 24 hours of incubation in a 37 ^° C. thermostat, pH was measured using a pH meter (ORIONSA 720, USA) based on BHI liquid medium to observe the effect of inhibiting acid production.

The control group was administered without adding the extract.

2-2. Streptococcus To Mutans  Inhibition Effect of Organic Acid Formation

Streptococcus mutans (0.5, 1, 2, 4, 8 mg / ml concentration of the sample to determine the inhibitory effect of the production of organic acids by S. mutans according to the addition of the moth extract according to Example 1 S. mutans ) was inoculated and measured for 24 hours after pH incubation with a pH meter, the results are shown in FIG.

The pH of the non-bacterial culture was about 7.2, but the pH dropped to 5.4 in the control group inoculated with bacteria only. In the experimental group to which the moth was added, it was observed that the pH drop was suppressed in a concentration-dependent manner. Therefore, it can be seen that the moth inhibits the production of organic acids by S. mutans in a concentration-dependent manner.

Experimental Example  3. Crumple Extract Streptococcus Mutans  Effect of inhibiting biofilm production

3-1. Experimental Method

BHI liquid medium and cypress extract were added to a 35mm dish and inoculated with 5x10 ⁵ CFU / ml / dish bacteria. After 24 hours incubation in 37 ℃ incubator all the supernatant was removed.

Each dish was washed with 1.5 ml of distilled water. Fixed in 2.5% glutaraldehyde solution (in 0.1M sodium cacodylate buffer, pH 7.2, 4) for 24 hours. Starting with 70% ethanol, the concentration was increased to 80%, 95%, and 100%, followed by washing and dehydration. After freeze-drying and coating with gold, SEM (Scanning Electron Microscopy) was observed and photographed.

3-2. Streptococcus Mutans  Inhibitory effect of biofilm production

3 is a diagram showing the experimental results of the inhibition of the production of Streptococcus mutans biofilm according to Experimental Example 3. ((A) Control (B) 0.5 mg / ml (C) 1 mg / ml (D) 2 mg / ml ( E) 4 mg / ml (F) 8 mg / ml (G) Positive control (0.1% NaF), Bar = 50 um)

S. mutans of Streptococcus mutans biofilm As a result of using the electron microscope to inhibit the production, it can be seen that the control group that is not treated with the extract extract has formed a lot of biofilm, and as the concentration of the extract extract increases, the production of the biofilm is surely suppressed even under the electron microscope image. Able to know.

Claims (5)

Ixeris dentata NaKai) oral bacterium inhibiting composition containing the extract as an active ingredient.
The method of claim 1,
The moth extract is a composition for inhibiting oral bacteria, characterized in that it inhibits the growth of Streptococcus mutans, the generation of organic acids by Streptococcus mutans and Streptococcus mutans biofilm.
Ixeris dentata Nakai) Preventing and treating dental diseases for inhibiting oral bacteria containing an extract as an active ingredient, and formulated in a pharmaceutical unit dosage form by adding a pharmaceutically acceptable carrier, excipient or diluent.
Ixeris dentata NaKai) extract as an active ingredient, mouthwash, mouthwash, toothpaste, oral spray and denture cleaning agent consisting of one or more formulations selected from the group consisting of dental disease prevention and treatment of dental diseases.
Ixeris dentata Nakai) Health functional food for oral bacteria inhibition with extract as an active ingredient, food additive acceptable food.

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