KR20110096779A - Composition containing the extract of buckwwheat bud, dropwort stem, chrysanthemum indicum, akebia quinata stem, lxeris dentata, plantain seed and bamboo leaf with effect of improving brain function - Google Patents

Abstract

The present invention is a composition for improving brain function, comprising a plant extract extracted by adding water as a solvent to the plant mixture consisting of buckwheat sprouts, buttercup stems, gamguk, keg, moth, tea and bamboo leaves as an active ingredient In this regard, the activity of the sympathetic β-receptor is changed to increase brain blood flow, thereby exerting an effect on improving brain function.

Description

Composition containing the extract of buckwwheat bud, dropwort stem, chrysanthemum indicum, akebia quinata stem, lxeris dentata, plantain seed and bamboo leaf with effect of improving brain function}

The present invention relates to a composition for improving brain function, and more particularly, to a composition for improving brain function, which contains plant extracts extracted from buckwheat sprouts, buttercup stems, gamguk, keg, moth, tea and bamboo leaves.

The brain is the backbone of life and maintains proper brain blood flow to obtain nutrients such as oxygen and glucose, and to maintain proper function by removing waste products such as carbon dioxide (Kim Ki-seok. 1989. Brain. Seowonsa, Seoul. P. 49-50.). Average cerebral blood flow and brain metabolism are not significantly affected by daily life, but local and local brain blood flow is required in certain parts of the brain associated with it by physical and mental activities. In other words, proper blood flow changes in certain parts of the brain are required for the smooth operation of the human body (Kim Ki-seok. 1989. Brain. Seowonsa, Seoul. P 49-50, Sung Ho-kyung. 1996. Physiology. 110.).

On the other hand, cerebral ischemia due to lack of blood flow must reach a certain threshold in order to cause neurological and morphological damage to the brain.The extent and duration of early reperfusion play an important role during the development of necrosis and infarction. is (Slomiany BL. 2004. Platelet activation mediates fator porphyromonas gingivalis lipopolysaccharide interference with salivary mucin synthesis via phosphatidylinositol 3-kinase dependent constitutive nitric oxide synthase activation. J Physiol Pharmacol 8: 5-98.).

Pathophysiological processes of cerebral ischemia can be classified into three stages based on changes in cerebral blood flow (Korean Society for Neurosurgery. 1988. Neurosurgery. Jinsu Publishing, Seoul, p 303-305.). The first stage is a period of early perfusion failure, in which the tissue is incomplete but infarction, where oxygen consumption and glucose metabolism in the localized brain are fully or relatively preserved and fully recovered. . The second stage is characterized by regional cerebral blood flow (rCBF), which goes beyond the reduction of the metabolic requirements of the tissue. The third stage is the 'permanent infarct' state, the last stage of cerebral ischemic injury, with a decrease in metabolic and local cerebral blood flow. In the area around the brain tissue necrosis caused by the disorder of cerebral blood flow, there is a region where the brain blood flow is maintained at 15-20 mL / 100 g / min. Brain function may improve.

As described above, the brain tissue is deteriorated or stopped depending on the degree of decrease in the blood flow of the brain and causes severe brain blood flow necrosis when the disorder continues. On the contrary, when cerebral blood flow is normal, cerebral blood flow is normalized and the necrosis of brain tissue is avoided (Ogun. 1952. Internal Medicine. Namsandang, Seoul. P 123-127, Lee Dudal. 1990. Pathology as illustrated. Korea Medicine, Seoul. p 740-743.).

Therefore, maintenance of a constant cerebral blood flow in a normal state or a disease state is a very important factor for the prevention as well as the treatment of brain diseases.

On the other hand, the composition for improving brain function to increase the reduced brain blood flow when the brain function is reduced, it has yet to be developed to include an extract of a plant mixture consisting of buckwheat sprouts, buttercup stems, gamguguk, keg, moth, tea and bamboo leaves It has not been done.

Accordingly, an object of the present invention is to develop and provide a brain function improving composition containing a plant extract of buckwheat sprouts, buttercup stems, gamguk, keg, moth, tea and bamboo leaves as an active ingredient.

In order to achieve the above object, the present invention is characterized by containing the plant extract extracted by adding water as a solvent to the plant mixture consisting of buckwheat sprouts, buttercup stems, gamguk, keg, moth, chajeon and bamboo leaves as an active ingredient It provides a composition for improving brain function.

Hereinafter, the means for solving the problems of the present invention will be described in detail.

Brain function decreases or stops depending on the degree of decrease in cerebral blood flow in brain tissues, and severe brain blood flow disorders lead to necrosis of brain tissues. However, after cerebral blood flow disorder, normalization of brain blood flow avoids necrosis of brain tissues. Thus, maintenance of a constant brain blood flow is a very important factor for prevention and treatment of brain diseases.

Therefore, the present invention is a method for improving brain function, the solvent in the plant mixture consisting of buckwheat sprouts, buttercup stems, gamguk, keggu, moth, tea and bamboo leaves to increase the local cerebral blood flow when the brain blood flow is reduced It provides a composition for improving brain function, comprising the plant extract extracted by adding water as an active ingredient.

Buckwheat sprout contains a lot of rutin (vitamin P) than buckwheat seeds and is rich in fiber and inorganic ingredients such as calcium (Ca), potassium (K) and magnesium (Mg).

Buttercup stem has a unique aroma and taste due to essential oils, and is rich in minerals such as vitamin A and vitamin C, calcium, iron, beca-carotene and folic acid.

Gamkook has been used for folk remedies such as wind fever, dizziness, pain in the head, redness and tears in the eyes, swelling in the eyes, joint pain and hypertension. terpenoid) and phenolic compounds.

Throat is a stem of an ivy or other homologous plant, which is made by removing the bark and cutting it horizontally.In Chinese medicine, it is used as an anti-inflammatory medicine, diuretic medicine, analgesic medicine, and pear pesticide. Used.

It is known that it contains 80 kinds of volatile flavor essential oils, and according to consent, it is effective in calming, hypnosis, hematopoiesis, dryness, indigestion, hepatitis, and appetite.

The teapot is the word for the plantain's seed, which is used to control diarrhea, to clear the eyes, and to stop coughing.

Bamboo leaves are water 43.4%, crude protein 10.8%, crude fat 3.1%, mineral 4.3%, soluble nitrogen free 37.9%, vitamin C (in 100 g) 1,278 mg, aspartic acid, glutamic acid, serine, threonine, florin, alanine, It contains amino acids such as cysteine and phenylalanine, and sugars such as xylose, arabinose, glucose, mannose, and galactose, and is used for the treatment of vomiting, stomach fever, hemorrhage, and swelling.

On the other hand, the experiment described in the following experimental example was performed to determine what mechanism the composition for improving brain function of the present invention increases the local cerebral blood flow, by treating propranolol (propranolol), a sympathetic β receptor blocker, When the plant extract of the present invention was treated, it was confirmed that the decreased local cerebral blood flow increased significantly. From this it can be inferred that the composition for improving brain function of the present invention increases local cerebral blood flow by changing the activity of the sympathetic β receptor.

On the other hand, when water is added to the plant mixture as a solvent, water is preferably added in an amount of 2.7 to 4.7 parts by weight based on 1 part by weight of the plant mixture.

Meanwhile, the plant mixture is preferably 26.4 to 48.4% by weight of buckwheat sprout, 26.4 to 48.4% by weight of buttercup stem, 1.4 to 2.4% by weight of soybean paste, 1.4 to 2.4% by weight of neck, 10 to 18% by weight of moth, 1.9 to 3.5% of char. % And bamboo leaves 3.3 to 6.1% by weight is good.

As described above, the composition for improving brain function of the present invention exerts an effect of improving brain function by increasing the amount of cerebral blood flow by changing the activity of the sympathetic β receptor.

1 is a diagram showing the changes in local cerebral blood flow and mean blood pressure by concentrations of plant extracts.

Hereinafter, the content of the present invention will be described in more detail in the following examples, but the scope of the present invention is not limited only to the following examples, and includes modifications of equivalent technical ideas.

Example  1: plant extract manufacturer

Based on 4 liters of water, add 400 g of buckwheat sprout, 400 g of buttercup stem, 20 g of soybean paste, 20 g of wood, 20 g of ginseng, 150 g of charcoal, 30 g of bamboo tea, and 50 g of bamboo leaves Obtained.

In Experimental Example 1 and Experimental Example 2, the plant extract obtained in Example 1 was filtered with cotton cloth, and then concentrated under reduced pressure (CCA-1100, Eyela, Tokyo, Japan) and rapidly frozen and dried at -70 ° C (PVTFA 10AT, ILSIN). , Korea) to prepare a powder and used as a sample. The extract powders were prepared at each concentration (0.01 mg / kg, 0.1 mg / kg, 1.0 mg / kg and 10.0 mg / kg, i.p.) using saline and then injected into the femoral vein.

The experimental animals were placed in a standard solid feed in a laboratory (Temperature 22.5 ℃ ~ 24.0 ℃, Humidity 57.0 ~ 59.0%) equipped with a constant temperature and humidity device for rats of 'Sprague-Dawley system (♂)' weighing about 250 g. Sam # 31, Samtako, Osan, Korea) and water were used after being adapted to the environment of the experiment for more than two weeks.

Statistical analysis of all data was performed using the statistical analysis system (SAS) PC package and the analytical values were presented as mean ± standard deviation (mean ± SE). Significant differences between the group not administered with the extract and the group administered with the extract at each concentration were tested by 'paired t- test'.

Experimental Example  1: topical Cerebral blood flow  Changes in blood pressure and mean blood pressure

Local cerebral blood flow was measured using a 'Laser-Doppler flowmeter system' (Transonic Instrument, Chicago, U.S.A.).

Rats were anesthetized with urethane (urethane, 750 mg / kg, i.p.) and maintained at 37-38 ° C, and then fixed in a 'stereotractic frame'. After fixation, the scalp was cut along the midline to expose the parietal bone, and then a craniotomy of 5-6 mm in diameter was performed at the 4-6 mm lateral and -2-1 mm front of the 'brema'. At this time, the thickness of the parietal bone was left as thin as possible to prevent epidural bleeding. Using the 'stereotactic micromanipulator' to align the 'neddle probe' (0.8 mm diameter) for the 'Laser-Doppler flowmeter (Transonic Instrument, USA)' perpendicular to the cerebral cortex surface, After stabilizing for a while, the extract prepared above was administered at each concentration (0.01 mg / kg, 0.1 mg / kg, 1.0 mg / kg and 10.0 mg / kg, ip). After administration, regional cerebral blood flow (rCBF) was measured for 30 minutes. One rat was measured once, and a total of 10 rats were tested at one concentration for a total of 10 rats, and the average value was used as the average local cerebral blood flow.

On the other hand, the average blood pressure change measurement method was as follows.

Rats were anesthetized with urethane (urethane, 750 mg / kg, i.p.) 'and fixed with supine on a heat pad to maintain body temperature at 37-38 ° C. To monitor systemic blood pressure fluctuations, blood pressure was measured using a pressure transducer connected to a polyethylene tube inserted into the femoral artery of the experimental animal (grass model 7E polygraph, Rhode Island, USA). / 8e, AD instruments, England) and 'Macintosh computer (Power Macintosh 6100/66, Berkingkum, England)' for 30 minutes. One rat was measured once, and a total of 10 rats were tested 10 times for a concentration, and the average value was taken as the average blood pressure.

As a result of the measurement (FIG. 1), when the baseline cerebral blood flow base value of normal rats not administered the plant extract was converted to 100.00 ± 9.27%, it was applied to the rats at the concentration of 0.01 mg / kg and 0.1 mg / kg of the extract. Cerebral blood flow increased from baseline to 103.41 ± 10.54% and 108.24 ± 22.47%, respectively, and local cerebral blood flow at the time of 1.0 mg / kg and 10.0 mg / kg extract was 113.41 ± 33.07% and 125.18 ± 27.84, respectively. Significantly increased (p <0.05).

On the other hand, when the mean blood pressure baseline of the normal rats not administered the plant extract was converted to 100.00 ± 0.06%, the mean blood pressure decreased by 99.60 ± 0.05% and 96.27 ± 0.07%, respectively. The mean blood pressure decreased by 93.27 ± 0.07% and 88.74 ± 0.06% at 1.0 mg / kg and 10.0 mg / kg (p <0.05).

Experimental Example  2: topical Cerebral blood flow  Of changes in blood pressure and blood pressure

In order to confirm the change in local cerebral blood flow and blood pressure by the administration of the plant extract for each concentration prepared in Example 1, pretreatment with various drugs related to blood flow and blood pressure, and administration of the extract, local cerebral blood flow The mechanisms of hypertension showed changes.

(1) topical Cerebral blood flow  Mechanism of Change

To determine whether the increase in local cerebral blood flow by plant extracts is related to the sympathetic β receptor, propranolol, a sympathetic β receptor blocker, was first administered at a concentration of 3 mg / kg, followed by 0.01 mg / kg, 0.1 mg / kg, 1.0 mg / kg and 10.0 mg / kg were administered at different concentrations and local cerebral blood flow was observed for 30 minutes.

In addition, in order to determine whether the increase in local cerebral blood flow caused by the plant extract is related to the action of the parasympathetic nervous system, after administration of atropine, a parasympathetic receptor blocker at a concentration of 10 mg / kg, the plant extract was 0.01 mg. Local cerebral blood flow was observed for 30 minutes by administration of / kg, 0.1 mg / kg, 1.0 mg / kg and 10.0 mg / kg.

In addition, intravenous injection of L-NNA (nitric oxide synthase inhibitor) at a concentration of 1.0 mg / kg was investigated to determine whether local cerebral blood flow increase by plant extracts is related to nitric oxide production. , The plant extracts were administered at concentrations of 0.01 mg / kg, 0.1 mg / kg, 1.0 mg / kg and 10.0 mg / kg to observe local cerebral blood flow for 30 minutes.

In addition, in order to investigate the effect of necrosis on cerebrovascular effects, methylene blue was administered intravenously at a concentration of 10 ug / kg as a guanylyl cyclase inhibitor of cyclic GMP, followed by plant extracts. Changes in local cerebral blood flow were observed for 30 minutes by administration of 0.01 mg / kg, 0.1 mg / kg, 1.0 mg / kg and 10.0 mg / kg.

In addition, in vitromethacin, a cyclo-oxygenase inhibitor, indomethacin 1.0 to determine whether the increase in local cerebral blood flow by plant extracts is related to the production of cyclo-oxygenase. After pretreatment of mg / kg intravenously, the plant extracts were administered at concentrations of 0.01 mg / kg, 0.1 mg / kg, 1.0 mg / kg and 10.0 mg / kg to observe local cerebral blood flow for 30 minutes.

On the other hand, the brain blood flow was 100.00% (control) when only the drug was administered in each group and no plant extract was administered.

Local cerebral blood flow change, unit%
Propranolol +
Plant extract Atropine +
Plant extract L-NNA +
Plant extract Methylene blue +
Plant extract Indomethacin +
Plant extract Control group 100.00 ± 0.03 100.00 ± 0.02 100.00 ± 0.02 100.00 ± 0.02 100.00 ± 0.02 0.01 mg / kg 105.91 ± 0.03 105.07 ± 0.09 106.05 ± 0.13 101.55 ± 0.24 103.19 ± 0.02 0.1 mg / kg 110.62 ± 0.05 111.21 ± 0.15 110.85 ± 1.06 104.52 ± 0.47 109.00 ± 0.05 1.0 mg / kg 116.93 ± 0.07 ^* 118.51 ± 1.05 120.19 ± 2.17 118.17 ± 0.04 113.57 ± 0.06 10.0 mg / kg 126.97 ± 0.07 ^* 127.70 ± 0.17 128.07 ± 1.05 129.28 ± 0.47 125.82 ± 0.06  *: It is statistically significant compared to the control group (p <0.05)

As a result of measurement (Table 1), when the cerebral blood flow after prepranolol pretreatment was converted to 100.00%, plant extracts were prepared by 0.01 mg / kg, 0.1 mg / kg, 1.0 mg / kg, and 10.0 mg / kg concentrations, respectively. At the time of administration, local cerebral blood flow increased to 105.91%, 110.62%, 116.93% and 126.97%, respectively, especially when the concentrations of 1.0 mg / kg and 10.0 mg / kg were significantly increased. there was.

When the plant extract was administered after pretreatment with atropine, the cerebral blood flow was 105.07% of the control group when the concentration of the plant extract was 0.01 mg / kg, and 111.21% of the control group and 1.0 mg / kg when the 0.1 mg / kg dose was administered. Local administration of cerebral blood flow increased to 118.51% of the control group and 127.70% of the control group to 10.0 mg / kg. However, there was no significant difference.

When the plant extract was administered after pretreatment with L-NNA, local cerebral blood flow was 106.05% of the control group when the concentration of the plant extract was 0.01 mg / kg, and 110.85% of the control group and 1.0 mg / kg when the 0.1 mg / kg dose was administered. When administered kg and 10.0 mg / kg, the blood flow was increased by 120.19% and 128.07% of the control group, respectively. However, there was no significant difference.

After administration of methylene blue, when the plant extracts were administered at concentrations of 0.01 mg / kg, 0.1 mg / kg, 1.0 mg / kg and 10.0 mg / kg, cerebral blood flow was 101.55% and 104.52%, respectively, compared to the control group. , 118.17% and 129.28%. However, there was no significant change in local cerebral blood flow.

After pretreatment with indomethacin, when injected with plant extracts, local cerebral blood flow increased to 103.19%, 109.00%, 113.57% and 125.82%, respectively, compared to the control group. However, there was no significant change in local cerebral blood flow.

(2) analysis of blood pressure lowering mechanism

 To investigate the mechanism of blood pressure drop in rats caused by plant extracts, propranolol (3 mg / kg, iv), a sympathetic β receptor blocker, was pretreated and plant extracts were treated with 0.01 mg / kg, 0.1 mg / kg, 1.0 mg. After each dose of / kg and 10.0 mg / kg concentration, the change in blood pressure was measured for 30 minutes.

In addition, 10 mg / kg of atropine, a blocker of parasympathetic receptors, was injected intravenously, and then 0.01 mg / kg, 0.1 mg / kg, 1.0 mg / kg and 10.0 mg / kg concentration was administered respectively, and the change in blood pressure was measured for 30 minutes.

In addition, after pretreatment with 1.0 mg / kg of L-NNA, a nitric oxide synthase inhibitor, to determine whether the mechanism of blood pressure drop in plant extracts is related to nitric oxide production, 0.01 mg / kg, 0.1 mg / kg, 1.0 mg / kg and 10.0 mg / kg of the extract were administered by concentration, and the change in blood pressure was measured for 30 minutes.

In addition, 10 ug / kg of methylene blue, a GC inhibitor (guanylyl cyclase inhibitor), was pretreated intravenously to determine whether the mechanism of blood pressure drop in plant extracts was related to the Gany (guanylyl cyclase) activity of the vascular muscle. After that, 0.01 mg / kg, 0.1 mg / kg, 1.0 mg / kg and 10.0 mg / kg of plant extracts were administered, respectively, and the change in blood pressure was measured for 30 minutes.

In addition, 1.0 mg / kg of indomethacin, a cyclooxygenase inhibitor, is injected intravenously to determine whether the mechanism of blood pressure drop in plant extracts is related to the production of cyclooxygenase. After treatment, 0.01 mg / kg, 0.1 mg / kg, 1.0 mg / kg and 10.0 mg / kg of plant extracts were administered, respectively, and the change in blood pressure was measured for 30 minutes.

On the other hand, the blood pressure when only the drug was administered in each group and the extract was not administered was 100.00% (control).

Blood pressure change analysis, unit%
Propranolol +
Plant extract Atropine +
Plant extract L-NNA +
Plant extract Methylene blue +
Plant extract Indomethacin +
Plant extract Control group 100.00 ± 0.03 100.00 ± 0.02 100.00 ± 0.02 100.00 ± 0.02 100.00 ± 0.02 0.01
mg / kg 99.69 ± 1.02 97.80 ± 0.09 97.09 ± 0.04 98.43 ± 0.17 97.00 ± 0.09 0.1
mg / kg 97.18 ± 0.07 94.44 ± 0.24 95.43 ± 0.05 96.27 ± 0.56 95.48 ± 1.37 1.0
mg / kg 93.20 ± 0.41 ^* 90.24 ± 1.57 92.64 ± 0.17 93.41 ± 0.84 91.96 ± 2.79 10.0
mg / kg 89.34 ± 2.14 ^* 83.89 ± 1.41 85.43 ± 2.71 84.11 ± 1.67 81.36 ± 0.29 *: It is statistically significant compared to the control group (p <0.05)

Results of measurement (Table 2), when the blood pressure after propranolol injection was the control group (100.00%), the blood pressure was 99.69% (0.01 mg / kg) and 97.18% (0.1 mg / kg) compared to the control group when the plant extract was administered. ), 93.20% (1.0 mg / kg) and 89.34% (10.0 mg / kg). However, there was no significant drop in blood pressure.

In addition, when the plant extract was administered at 0.01 mg / kg, 0.1 mg / kg, 1.0 mg / kg and 10.0 mg / kg, respectively, after injection of atropine, blood pressure was 97.80% and 94.44%, respectively, compared to the control group. , 90.24% and 83.89%. However, there was no significant blood pressure drop.

In addition, when the plant extract was injected after pretreatment with L-NNA, when the concentration of the plant extract was 0.01 mg / kg, 97.09% compared to the control, 95.43%, 1.0 mg / kg, and 10.0 mg at 0.1 mg / kg. / kg at 92.64% and 85.43%, respectively, indicating a drop in blood pressure due to plant extract administration. However, there was no significant difference.

In addition, after the injection of methylene blue (Methylene blue), when the plant extract is injected, when the concentration of the plant extract is 0.01 mg / kg 98.43% compared to the control, 96.27%, 1.0 mg / when 0.1 mg / kg In case of kg, 93.41% and 10.0 mg / kg were 84.11%, especially when the concentration of plant extract was 1.0 mg / kg and 10.0 mg / kg.

In addition, when indomethacin was administered, when the plant extract was injected, the concentration of the plant extract was 0.01 mg / kg, 97.00% compared to the control group, and 95.48%, 1.0 mg / kg, when the concentration was 0.1 mg / kg. And 10.0 mg / kg at 91.96% and 81.36%, respectively. However, there was no significant difference.

Claims (3)

A composition for improving brain function, comprising a plant extract extracted by adding water as a solvent to a plant mixture consisting of buckwheat sprouts, buttercup stalks, persimmon soup, tree trunks, moths, chalets and bamboo leaves.
The method of claim 1,
Water,
2.7 ~ 4.7 parts by weight based on 1 part by weight of the plant mixture composition for improving brain function
The method of claim 1,
Plant mixtures,
Buckwheat sprout 26.4 ~ 48.4% by weight, Buttercup stem 26.4 ~ 48.4% by weight, Gamju 1.4 ~ 2.4% by weight, Wood barrel 1.4 ~ 2.4% by weight, Mugwort 10-18% by weight, Charcoal 1.9 ~ 3.5% by weight, and bamboo leaves 3.3 ~ 6.1 Composition for improving brain function, characterized in that consisting of

Images