KR20100130020A - A composition comprising novel black ginseng without tumor promoting agents such as benzopyran and the extract thereof obtained by novel process - Google Patents

Abstract

PURPOSE: A method for preparing black insam (ginseng) and a health food including the same are provided to produce black ginseng with excellent quality because Rg3, Rk1, Rg5, Rh1, Rh2 and etc. exist in red ginseng in a trace amount. CONSTITUTION: A method for preparing black insam (ginseng) comprises the steps of: drying ginseng at 20 ~ 60°C for 24 ~ 96 hours so that a moisture content is 20 ~ 55%; steaming the ginseng at a temperature of 105 ~ 155°C under the pressure of 0.06 ~ 0.50MPa for 1 ~ 4 hours; and drying the ginseng at a temperature of 40 ~ 70°C for 24 ~ 48 hours so that a moisture content is within 14%.

Description

A composition comprising novel black ginseng without tumor promoting agents such as benzopyran and the extract particularly obtained by novel process}

The present invention is shortened compared to the conventional method for manufacturing black ginseng through the process of repeating drying and steaming at the appropriate temperature and time from ginseng, using high temperature and high pressure capable of mass production Rg ₃ , Rk ₁ , Rg ₅ , The present invention relates to a novel method for producing black ginseng of good quality, containing a large amount of saponins that are not present in trace amounts or trace amounts of red ginseng or red ginseng such as Rh ₁ , Rh _2, and the like, and hardly detectable carcinogenic substances such as benzopyran.

Reference 1 Okamura N. et al., Biol. Pharm. Bull. , 17 (2) , p.270, 1994

[Document 2] Republic of Korea Patent Registration No. 10-0543862

Cha HY et al., Biol Pharm Bull., 28 (9) , pp.1621-1625, 2006

Document 4 Kang JS et al., Arch Pharm Res . , 28 (3) , pp.335-342, 2005

[Reference 5] Kordella T et al., Diabetes Forecast , 57 (1) , pp.RG5-8, 2004

[6] Neuberger J et al., The Royal College of Physicians of London and the British Society of Gastroenterology ., 49 , Suppl 1, pp. I1-21, 2001

[Reference 7] Chung BC et al., Pharmacol Res . , 54 (1) , pp.46-49, 2006

[Reference 8] Huang C. et al., Sichuan Da Xue Xue Bao Yi Xue Ban , 37 (1) , pp. 60-62, 2006

Document 9 Kim DH et al., Biol Pharm Bull ., 28 (10) , pp. 1992-1994, 2005

[Reference 10] Sun YX et al., J Agric Food Chem ., 51 (9) , pp. 2555-2558, 2003

[11] Qui H et al., Zhonghua Wai Ke Za Zhi , 40 (8 ), pp. 606-608, 2002

12 Schini-Kerth VB et al., Eur J Pharmacol . , 367 (1) , pp. 51-57, 1999

[Reference 13] Lee HK et al., Biol Pharm Bull ., 21 (1) , pp. 79-80, 1998

Korean red ginseng has a historical record of about 1,000 years ago (there is a record of red ginseng in Goryeo-do, written by the Chinese Song Dynasty), and it is a numerical medicine processed and manufactured by numerical method, which is a traditional Chinese medicine. have. In general, the numerical objectives of herbal medicines are known as: 1) reducing side effects such as toxicity, 2) altering herbal properties and enhancing drug efficacy, 3) improving storage and storage, and 4) correcting taste and odor and color. In general, red ginseng undergoes a second ingredient conversion during an appropriate heat treatment process, thereby generating new active ingredients unique to red ginseng or fresh ginseng, and some active ingredients increase in content. As such, new ginsengs that do not exist in ginseng are produced according to the processing method of ginseng, and new processed ginseng can be developed that shows better effects on various physiological activities including anticancer activity than ginseng (Okamura N. et al., Biol . Pharm . Bull . , 17 (2) , p. 270, 1994).

In China, it has developed a method of mass-producing Rg ₃ , a ginseng saponin, which is rarely present in white ginseng and is present in small amounts in red ginseng, and is used for anticancer treatment under the trade name 'Shenyi'.

In addition, domestic bio venture companies are working hard to develop new ginseng-related materials to capture the $ 3.5 billion global ginseng market. Until now, the only company that has made ginseng standardized as a functional food and has been performing in the global market is the “Pharmton Company” under Beringer Ingelheim, Switzerland. National research on ginseng and commercialization of venture industry are in progress. .

'Hwangsam EX' co-developed by Korea Research Institute of Biotechnology and biotechnology venture Vitijin, Namjin Farm Ginseng Farming Co. Several types of processed ginseng are being developed, such as Biomax, which was developed jointly with UPI in the United States, Six Plus of CJ, and Yellow Ginseng of Ildong Pharmaceutical.

In addition, recently, the principle of Gujeunggupo (Korean Patent Registration No. 10-0543862) black ginseng, which is the most representative method, is to use water and fire together in the processing method of Chinese herbal medicine, one of the steaming methods. There is a bar.

However, the above method may cause problems such as carbonization in the process of steaming and drying nine times. In fact, carcinogenic substances such as benzopyrans are detected in black ginseng prepared in such a method, causing social controversy. In addition, if the black ginseng is manufactured under the conditions of high temperature and high pressure without drying the ginseng, the fresh ginseng may burst during the manufacturing process, and thus, black ginseng of excellent quality cannot be prepared.

Therefore, the present invention is reduced in time compared to the method of agglutination vesicles using ginseng, mass production is possible, not bursting, not carbonized, saponins such as Rg ₃ , Rk ₁ , Rg ₅ , Rh ₁ , Rh ₂ The present invention was completed by preparing a good quality black ginseng containing a large amount.

In order to achieve the above object, the present invention provides a novel method for producing black ginseng using high temperature and high pressure, which is shortened in time through a process of drying, steaming, heating and drying ginseng.

Hereinafter, the present invention will be described in detail.

Black ginseng of the present invention can be prepared as follows.

According to the present invention, the ginseng is washed with an ultrasonic cleaner and dried at a temperature of 20 to 60 ° C., preferably 45 to 60 ° C., for 24 to 96 hours, preferably 30 to 48 hours, so that the moisture content is 20 to 55%. Preferably a primary drying step of 25 to 35%; Steaming process for 1 to 4 hours, preferably 2 to 3 hours at a temperature of 105 to 155 ° C, preferably 110 to 135 ° C under a pressure of 0.06 to 0.50 MPa, preferably 0.10 to 0.25 MPa; It provides a novel manufacturing method of black ginseng comprising a secondary drying step of drying within 14% water content for 24 to 48 hours, preferably 30 to 40 hours at a temperature of 40 to 70 ℃, preferably 50 to 60 ℃ do.

The black ginseng prepared by the manufacturing method is dried through a drying method common in the art such as shade drying or freeze drying, and then pulverized and powdered into fine particles, preferably a particle size of about 50 μm to 200 μm. Through the use of excipients or carriers well known in the art can be prepared in compositions such as pills, capsules, tablets.

The black ginseng prepared by the above method may be used as a raw material for various preparations by pulverizing into fine powder, preferably about 50 to 200 μm in size, using a herbal medicine grinder.

In addition, the method for obtaining black ginseng extract in detail, after pulverizing the black ginseng prepared by the above production method, by adding about 3 to 7 times, preferably 4 to 6 times (v / w) water of black ginseng, After standing at room temperature for 3 to 7 hours, 0.5-2 L of a solvent selected from water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof was added thereto, and the mixture was extracted under reflux three times for 3 hours, and then cooled to room temperature and filtered. The black ginseng extract of the present invention may be obtained by a method of removing ethanol using a vacuum concentrator.

The black ginseng prepared by the preparation method has a large amount of saponins such as Rg ₃ , Rk ₁ , Rg ₅ , Rh ₁ , and Rh ₂ present in trace amounts in red ginseng. Among the saponin components, Rg ₅ has various pharmacological activities such as anticancer effect, antidiabetic effect, and Rg ₃ has anti-stress effect, hepatoprotective effect and antioxidant effect. Can be. In addition, as a result of measuring the content of benzopyran, a carcinogenic substance present in the prepared black ginseng, it was confirmed that it is detected at 2ppb or less.

The production method of the present invention can be applied to ginseng leaves as well as ginseng itself. Ginseng leaves have not been used for medicinal purposes, but have been discarded or used for feed, and some of them have been used as raw materials for cosmetics or foods.However, when ginseng leaf extract is heated and pressurized in the same way as in the present invention, Since it is much stronger, it can be used for medicinal purposes as well as for the use of ginseng leaves.

The black ginseng prepared by the above method is characterized in that the existing ginseng, white ginseng, or red ginseng does not exist or enhances the content of the active ingredient present in trace amounts.

The present invention is prepared by the production method described above is rarely detected carcinogenic substances such as benzopyran and black ginseng or its extract containing a large amount of saponins, such as Rg ₃ , Rk ₁ , Rg ₅ , Rh ₁ , Rh ₂ as an active ingredient Provides health functional foods containing.

The composition comprising the black ginseng obtained by the manufacturing method of the present invention or its extract may be used in a variety of drugs, food and beverages. The food to which the black ginseng or the extract of the present invention may be added includes, for example, various foods, beverages, gums, teas, vitamin complexes, health supplements, and the like, and are powders, granules, tablets, capsules, or beverages. Can be used as

At this time, the amount of the black ginseng or its extract in the food or beverage may be generally added at 0.01 to 15% by weight of the total food weight, the health beverage composition is 0.02 to 10 g, preferably 0.3 to 1 based on 100 ml It can be added in the ratio of g.

In addition to containing the black ginseng or its extract as an essential ingredient in the indicated ratio, the health beverage composition of the present invention does not have any particular limitation on the liquid component and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. Can be. Examples of the above-mentioned natural carbohydrates are conventional monosaccharides such as disaccharides such as glucose and fructose, such as maltose, sucrose and the like, and polysaccharides such as dextrin, cyclodextrin and the like. Sugars and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (e.g., Rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of natural carbohydrates is generally about 1 to 20 g, preferably about 5 to 12 g per 100 mL of the composition of the present invention.

In addition to the above-mentioned composition, the composition of the present invention can be used as a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and intermediates (cheese, chocolate etc.), pectic acid and its salts, Salts, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages and the like. In addition, the compositions of the present invention may contain flesh for the production of natural fruit juices and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical, but is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

The manufacturing method of the black ginseng developed by the present invention uses a high temperature and high pressure, which is significantly shorter in time and mass-produced, compared to the existing augmentation vesicles, under the conditions of constant temperature, pressure and water content obtained through the present invention. In the case of manufactured black ginseng, there are few defects such as bursts occurring during the manufacturing process, and a large amount of Rg ₃ , Rk ₁ , Rg ₅ , Rh ₁ , Rh _2, etc., present in trace amounts in red ginseng results in excellent quality. It can provide breakthrough black ginseng.

Hereinafter, the present invention will be described in detail with reference to the following examples and experimental examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.

Comparative Example  One. Gujeungpo  Preparation of Black Ginseng Extract

1kg of 5 year old ginseng grown in Geumsan was purchased (Gumsan Ginseng Combination), put into an ultrasonic cleaner and washed repeatedly three times for 15 minutes. The washed ginseng was put into a steamer and steamed for 1 hour at 95 ° C except for the preheating time. The preheating time is typically 30 minutes, which is the time from the steamer to the water vapor, and after maintaining the internal temperature of the dryer with built-in heating wire at 60 ℃, rotate the pen evenly for the first time. Tea was dried.

By repeating the steaming and drying process 9 times as described above, a black ginseng having a water content of 14% or less was changed to black through white ginseng.

10 g of the black ginseng prepared above was taken, extracted three times by the following extraction method, and injected into HPLC for analysis. That is, 10 g of black ginseng was weighed and mixed with 100 ml of hexane. Then, 50 ml of water: DMF mixed solution (1: 9) was added and shaken uniformly to separate DMF and hexane. 25 ml of DMF mixture was added to the hexane layer and extracted. This operation was repeated twice to bring the total DMF to 100 ml. 100 ml of 1% sodium sulfate solution was added to the separated DMF mixed layer, and the mixture was sufficiently mixed. Then, 50 ml of hexane was added thereto, followed by extraction. The hexane layer was washed three times with distilled water, dehydrated with anhydrous sodium sulfate, and concentrated under reduced pressure at 40 ° C. to use as a sample of carcinogenic substance (hereinafter referred to as GS).

Comparative Example  2. Using high temperature and high pressure directly in fresh ginseng Preparation of Black Ginseng

1kg of 5 year old ginseng grown in Geumsan was purchased (Gumsan Ginseng Combination), put into an ultrasonic cleaner and washed repeatedly three times for 15 minutes. The washed ginseng was put into a steamer and steamed for 3 hours at 119 ° C under pressure (0.13 MPa) except for the preheating time. The preheating time is usually 30 minutes from the steamer to the water vapor, and the tissue was destroyed because the ginseng was pressed without drying during steaming, and the active ingredient, such as saponin, flowed out of the tissue. . After maintaining the internal temperature of the dryer with a built-in heating wire at 60 ℃, the first ginseng evenly steamed while rotating the pen was first dried for 12 hours.

Comparative Example  3. Preparation of Red Ginseng Extract

1kg of 5 year old ginseng grown in Geumsan was purchased (Gumsan Ginseng Combination), put into an ultrasonic cleaner and washed repeatedly three times for 15 minutes. The washed ginseng was put into a steamer and steamed for 3 hours at 95 ° C except for the preheating time. The preheating time is usually 30 minutes, which is the time from the steamer to the water vapor.The red ginseng is dried for 48 hours while keeping the internal temperature of the dryer with built-in wire at 55 ℃ and rotating the pen evenly. 256 g was obtained.

50 g of the prepared red ginseng was pulverized, and 1L of 80% ethanol was added thereto, and the mixture was extracted at reflux three times for 4 hours. 300 ml of water was added to the red ginseng crude extract and suspended. Then, 300 ml of ether was added to remove the nonpolar substance. 4.8 g of red ginseng extract containing a large amount of ginseng saponin was obtained and used as a sample for measuring saponin content (hereinafter referred to as RGS).

Example  1. Preparation of Black Ginseng

1kg of 5-year-old ginseng grown in Geumsan was purchased (Gumsan Ginseng Combination), put into an ultrasonic cleaner, and washed repeatedly three times for 10 minutes. The washed ginseng was first dried at 55 ° C. for 36 hours to prevent tissue from bursting during the manufacturing process of black ginseng, as in Comparative Example 2, so that the moisture content was 30%. First, the ginseng with 30% moisture content is steamed again (0.13MPa) at 119 ° C for 3 hours, and then dried for 2 hours at 55 ° C with a dryer for 2 hours. Was prepared.

10 g of the black ginseng prepared above was taken, extracted three times by the following extraction method, and injected into HPLC for analysis. That is, 10 g of black ginseng was weighed and mixed with 100 ml of hexane. Then, 50 ml of water: DMF mixed solution (1: 9) was added and shaken uniformly to separate DMF and hexane. 25 ml of DMF mixture was added to the hexane layer and extracted. This operation was repeated twice to bring the total DMF to 100 ml. 100 ml of 1% sodium sulfate solution was added to the separated DMF mixed layer, and the mixture was sufficiently mixed. Then, 50 ml of hexane was added thereto, followed by extraction. The hexane layer was washed three times with distilled water, dehydrated with anhydrous sodium sulfate, and concentrated under reduced pressure at 40 ° C. to use as a sample of carcinogenic substance (hereinafter referred to as NGS).

Example  2. Preparation of Black Ginseng Extract

Take 20 g of black ginseng prepared by the method of Example 1, add 100 ml of water, and leave it at room temperature for 5 hours, extract 1 ml of 80% ethanol (20% water) and reflux 3 times for 3 hours to extract the extracted solution. After cooling to room temperature and filtered, ethanol was removed using a vacuum concentrator to obtain a black ginseng extract (5.76g). 500 ml of water was added to the black ginseng extract and suspended, and 500 ml of ether was added to remove the nonpolar material. 500 ml of saturated butanol was added to the remaining aqueous layer, followed by extraction of saponin present in ginseng four times, followed by removing butanol with a reduced pressure concentrator to obtain a butanol extract (crude saponin) containing a large amount of ginseng saponin (1.98 g).

This black ginseng crude saponin extract was used as a sample for saponin component analysis (hereinafter referred to as NGSS).

Experimental Example  1. Saponin Content Analysis of Black Ginseng

The saponin content of the red ginseng extract (RGS) obtained in Comparative Example 3 and the black ginseng saponin extract (NGSS) obtained in Example 2 was analyzed in the HPLC analysis conditions of Table 1 below, and the results are shown in Table 2 below. Shown in

Column: Shiseido C18 column (5㎛, 150 × 4.6mm) Flow rate 1ml / min Detector ELSD-LT detector Solvent A; CH ₃ CN: H ₂ O: 5% CH ₃ COOH = 15: 80: 5, B; CH ₃ CN: H ₂ O = 80:20
0 minutes (0% B), 0-10 minutes (30% B), 10-25 minutes (50% B), 25-40 minutes (100% B), 40-50 minutes (100% B), 50- 55 minutes (0% B), 55-58 minutes (0% B) Temperature 40 degrees

RGS (Comparative Example 3)% NGSS (Example 2)% Rg ₃ 0.066 0.98 Rh ₁ 0.069 0.094 Rh ₂ 0.051 0.085 Rk ₁ + Rg ₅ 0.075 1.19

As a result, as shown in Table 2, red ginseng contained a small amount of Rg ₃ , Rh ₁ , Rh ₂ and Rk ₁ + Rg ₅ as 0.066%, 0.069%, 0.051% and 0.075%, respectively. In black ginseng, Rg ₃ , Rh ₁ , Rh _2, and Rk ₁ + Rg ₅ were 0.98%, 0.094%, 0.085%, and 1.19%, respectively, and Rg ₃ and Rk ₁ + Rg ₅ were 15 times more present.

Experimental Example 2. Analysis of benzopyran content, a carcinogenic substance present in black ginseng

2-1. Analyzer condition

HPLC was used for the Waters alliance system, excitation wavelength 294nm, fluorescence wavelength 404nm, column temperature was 30 ℃, acetonitrile: tertiary distilled water (80:20) as a mobile phase, the flow rate was quantified at 1.0 mL / min.

2-2. Linearity, Detection Limit (LOD) and Quantitative Limit (LOQ)

50 mg of benzopyrene standard is precisely measured, dissolved in methanol to make exactly 50 ml, and then diluted to prepare a standard stock solution. Diluted to prepare a standard solution for each concentration of 7.8125, 15.625, 31.25, 62.5 and 125 ng / ml, and the peak area according to the analysis conditions. The calibration curve was prepared by obtaining. From the calibration curve, the linear correlation coefficient (standard: R ² = 0.99 or more) was obtained, and the linearity was examined.

The detection limit (LOD) and the quantitative limit (LOQ) were calculated using the following equations (1) and (2) by using a portion having a good linearity range of the calibration curve.

Detection limit (LOD) = 3.3 × σ / S (S / N = 3)

Limit of Quantitation (LOQ) = 10 × σ / S (S / N = 10)

(σ is the mean standard deviation of the intercepts, and s is the mean of the slopes.)

Benzopyran concentration (ug / Kg, ppb) GS (Comparative Example 1) 2.34 ± 0.065 NGS (Example 1) 0.32 ± 0.007

As a result of analyzing the content of benzopyran, a carcinogenic substance present in the black ginseng extract, as shown in Table 3, the black ginseng (GS) prepared by the method of erythropoietic cell in Comparative Example 1 exceeded 2 ppb as a reference value. Benzopyran was detected. On the other hand, black ginseng (NGS) prepared by using the method of high temperature and high pressure in Example 1, it was confirmed that benzopyran is detected below the reference value of 0.32ppb.

From the above results, the black ginseng (NGS) prepared by the method of the present invention not only kept the original shape of ginseng without bursting during the manufacturing process, and almost no carcinogenic substance such as benzopyran, which was recently controversial, was not detected. It was confirmed that black ginseng of good quality could be prepared.

On the other hand, Rg _{5, which} is produced specifically by steaming, has an anti-anxiety effect (Cha HY et al., Biol Pharm Bull., 28 (9) , pp.1621-1625, 2006), brain function improvement effect (Kang JS et al., Arch Pharm Res. , 28 (3) , pp.335-342, 2005), antidiabetic effects (Kordella T et al., Diabetes Forecast, 57 (1) , pp.RG5-8, 2004) and hepatitis C inhibitory effects (Neuberger J et al., the Royal College of Physicians of London and the British Society of Gastroenterology., 49, Suppl 1, pp.I1-21, 2001) , etc., Rg ₃ is anti-stress effect (Chung BC et al., Pharmacol Res. , 54 (1) , pp.46-49, 2006), anticancer effect (Huang C. et al., Sichuan Da Xue Xue Bao Yi Xue Ban, 37 (1) , pp. 60 -62, 2006), hepatoprotective effect (Kim DH et al., Biol Pharm Bull., 28 (10) , pp. 1992-1994, 2005), antioxidant effect (Sun YX et al., J Agric Food Chem., 51 (9) , pp.2555-2558, 2003), angiogenesis inhibitory effect (Qui H et al., Zhonghua Wai Ke Za Zhi, 40 (8 ), pp.606-608, 2002), vasorelaxant effect (Schini -Kerth VB et al., Eur J Pharmacol., 367 (1), pp.51-57, 1999) Platelet aggregation inhibitory action (Lee HK et al., Biol Pharm Bull., 21 (1), pp.79-80, 1998) it is known to exhibit various physiological activities that heuksam this saponin-containing large amounts, such as the above, a variety of Biological activity can be expected.

Experimental Example 3. Acute Toxicity Test

Acute toxicity test was performed using 6-week-old specific pathogen-free (SPF) SD rats. Two animals of each group were orally administered to the black ginseng extract of Example 2 at a dose of 1 g / kg. After administration of the test substance, mortality, clinical symptoms, and changes in body weight were observed. Hematological and hematological examinations were performed. Necropsy was performed to visually observe abnormalities in organs and thoracic organs.

As a result, no clinical symptoms or dead animals were observed in all animals treated with the test substance, and no toxic changes were observed in weight changes, blood tests, blood biochemical tests, and autopsy findings.

Therefore, the extract of the present invention does not show a toxic change in each rat up to 1 g / kg, oral administration minimum dose (LD ₅₀ ) was determined to be a safe substance of more than 1 g / kg.

The preparation examples of the composition comprising the black ginseng and extracts of the present invention will be described, but the present invention is not intended to be limited thereto, but is intended to be described in detail.

Formulation Example 1 Preparation of Powder

Black ginseng 20 mg of Example 1

Lactose 100 mg

Talc 10 mg

The above ingredients are mixed and filled in an airtight cloth to prepare a powder.

Formulation example  2. Preparation of Tablets

Black ginseng crude saponin extract 10 mg of Example 2

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, tablets are prepared by tableting according to a conventional method for preparing tablets.

Formulation example  3. Manufacture of capsule

Black ginseng of Example 1 10 mg

3 mg of crystalline cellulose

Lactose 14.8 mg

Magnesium Stearate 0.2 mg

According to a conventional capsule preparation method, the above ingredients are mixed and filled into gelatin capsules to prepare capsules.

Formulation example  4. Preparation of injections

Black ginseng crude saponin extract 10 mg of Example 2

180 mg mannitol

Sterile sterilized water for injection 2974 mg

Na ₂ HPO ₄ , 12H ₂ O 26 mg

According to the conventional method for preparing an injection, the amount of the above ingredient is prepared per ampoule (2 ml).

Formulation example  5. Liquid  Produce

Black ginseng 20 mg of Example 1

10 g of isomerized sugar

5 g of mannitol

Purified water

After dissolving each component in purified water according to the usual method of preparing a liquid solution, adding lemon flavor appropriately, mixing the above components, adding purified water, adjusting the whole to 100 ml by adding purified water, and then filling into a brown bottle. The solution is prepared by sterilization.

Formulation example  6. Manufacture of health food

1000 mg of black ginseng crude saponin extract of Example 2

Vitamin mixture quantity

70 [mu] g of vitamin A acetate

Vitamin E 1.0 mg

0.13 mg vitamin B1

0.15 mg of vitamin B2

0.5 mg vitamin B6

0.2 [mu] g vitamin B12

10 mg vitamin C

Biotin 10 μg

Nicotinic acid amide 1.7 mg

50 ㎍ of folic acid

Calcium pantothenate 0.5 mg

Mineral mixture quantity

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

15 mg of potassium phosphate monobasic

Secondary calcium phosphate 55 mg

Potassium citrate 90 mg

100 mg of calcium carbonate

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

Formulation example  7. Manufacture of health drinks

1000 mg of black ginseng of Example 1

Citric acid 1000 mg

100 g of oligosaccharide

Plum concentrate 2 g

Taurine 1 g

Purified water was added to a total of 900 ml

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was heated at 85 DEG C for about 1 hour with stirring, and the solution thus prepared was filtered to obtain a sterilized 2-liter container, which was sealed and sterilized, ≪ / RTI >

Although the composition ratio is a mixture of the components suitable for the preferred beverage as a preferred embodiment, the blending ratio may be arbitrarily varied according to the regional and national preferences such as the demand level, the demanding country, and the intended use.

Claims (3)

After washing the ginseng, the first drying step to dry for 24 to 96 hours at a temperature of 20 to 60 ℃ to make the water content 20 to 55%; Steaming process for 1 to 4 hours at a temperature of 105 to 155 ° C. under a pressure of 0.06 to 0.50 MPa; A method for producing black ginseng using high temperature and high pressure, including a secondary drying step of drying within 14% of water content for 24 to 48 hours at a temperature of 40 to 70 ° C, which is shortened and capable of mass production. After grinding black ginseng prepared by the production method according to claim 1, 3-7 times (v / w) water of black ginseng is added and left at room temperature for 3 to 7 hours, followed by water and lower carbon atoms 1-4. Extraction using a solvent selected from alcohol or a mixed solvent thereof as an extraction solvent. Black ginseng prepared by the method according to claim 1 or 2, containing little saponins such as Rg ₃ , Rk ₁ , Rg ₅ , Rh ₁ , and Rh ₂ , with little carcinogenic substances such as benzopyrans detected or the like Health functional food containing extract as an active ingredient.