KR101082262B1 - Composition comprising an extract of black ginseng showing anti-aging activity - Google Patents

Abstract

The present invention relates to a black ginseng extract obtained from black ginseng prepared using ginseng, and the extract of black ginseng exhibits an excellent antioxidant effect and can be used as an anti-aging pharmaceutical composition and health functional food.

Black ginseng, mass production, fresh ginseng, antioxidant.

Description

Composition comprising an extract of black ginseng showing anti-aging activity}

1 is a view showing the Onggi used for steaming black ginseng.

An object of the present invention is to provide an anti-aging pharmaceutical composition and health functional foods containing antioxidant activity containing black ginseng extract obtained from black ginseng prepared using ginseng as an active ingredient.

To capture the $ 3.5 billion global ginseng market, Korean bio venture companies are working hard to develop new global ginseng-related materials worth $ 3.5 billion annually. So far, the only company that has standardized ginseng as a functional food and is making a profit in the global market is “Pharmaton,” a company owned by Boehringer Ingelheim, Switzerland. National research on ginseng and commercialization of the venture industry are in progress.

Korean red ginseng has a historical record of about 1,000 years ago. have. In general, the numerical objectives of herbal medicines are known as: 1) reducing side effects such as toxicity, 2) altering herbal properties and enhancing drug efficacy, 3) improving storage and storage, and 4) correcting taste and odor and color. In general, red ginseng undergoes a second ingredient conversion during an appropriate heat treatment process, thereby generating new active ingredients unique to red ginseng or fresh ginseng, and some active ingredients increase in content. As such, new ingredients that do not exist in ginseng are produced according to the processing method of ginseng, and new processed ginsengs having better effects on various physiological activities including anticancer activity can be developed compared to ginseng (Okamura N. et al. , Biol . Pharm . Bull. , 17 (2) , p270, 1994).

In China, it develops a method of mass-producing Rg3, a ginseng saponin, which is rarely present in white ginseng and is present in red ginseng, and is used for anticancer treatment under the trade name 'Shenyi'.

In Korea, the representative product of functional ginseng is 'Seonsam' developed by bio venture company Ginseng Science in 1998.It was developed as Sesamsamjeong in 2001 and recognized in domestic health functional food market. It has been established as a leader in functional ginseng (Keum YS et al., Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng, Cancer Lett ., 150 (1) , pp 41-48, 2000).

In addition, 'Hwangsam EX' co-developed by Korea Research Institute of Biotechnology and biotechnology company Beatijin, Namseng Farm Co., Ltd., 'Ginseng Red Ginseng' produced by joining Biobio, Green Bio Co., Ltd., and Unigen Co., Ltd. Recently, various types of processed ginseng are being developed, such as Biomax, which was developed jointly with UPI in the US, Six Plus of CJ, and Yellow Ginseng of Ildong Pharm.

In addition, recently, as a result of efforts to further increase the active ingredient content of ginseng, black ginseng using the principle of Gugugugupo, which is the most representative method of using water and fire, was developed. (Korean Patent Laid-Open Publication No. 2003-0005089; manufacturing method of black ginseng and black rice ginseng), and in this laboratory, a standardized method of manufacturing black ginseng which can be shortened in time and mass production is possible (Korean Patent Application No. 10-2006- New black ginseng production method and composition containing the same) and black ginseng leaf manufacturing method (Korean Patent Application No. 10-2006-76232; Black ginseng leaf produced by the new manufacturing method and an anti-aging pharmaceutical composition containing the extract thereof and Health functional food) has been applied for a patent.

     As such, the present inventors have completed the present invention by confirming that black ginseng extract contains a large amount of ginseng saponin and exhibits an excellent antioxidant effect.

An object of the present invention is to provide an anti-aging pharmaceutical composition and health functional food containing antioxidant activity containing black ginseng extract obtained from black ginseng prepared using ginseng.

In order to achieve the above object, the present invention is an anti-aging pharmaceutical composition and health functional food containing antioxidant activity containing black ginseng extract obtained from black ginseng prepared by drying, steaming, heating and drying ginseng as an active ingredient. To provide.

Hereinafter, the present invention will be described in detail.

Black ginseng and extracts thereof of the present invention may be prepared through the following process.

After washing 3 to 7 years old root, preferably 5 years old root ginseng of the present invention, primary drying is carried out at 20 to 60 ° C, preferably 35 to 55 ° C for 12 to 36 hours, preferably 20 to 28 hours. fair; Primary steaming step of steaming for 3 to 9 hours, preferably 5 to 7 hours at a temperature of 60 to 120 ℃, preferably 85 to 105 ℃ except for preheating time in the Onggi; A secondary drying step of drying at a temperature of 40 to 80 ° C., preferably 50 to 70 ° C. for 6 to 18 hours, preferably 9 to 15 hours; A secondary steaming step of steaming at a temperature of 85 to 135 ° C, preferably 95 to 125 ° C for 3 to 9 hours, preferably 5 to 7 hours; Black ginseng may be prepared, including a third drying step of drying for 8 to 16 hours, preferably 10 to 14 hours.

In addition, the method for obtaining the extract of the black ginseng prepared by the above method will be described in detail. After crushing the black ginseng prepared by the above method, water of about 3 to 7 times, preferably 4 to 6 times the mass of black ginseng is added thereto. After standing at room temperature for 3 to 7 hours, 0.5-2 L of methanol was added and the mixture was extracted under reflux three times for three hours. The extracted solution is cooled to room temperature, filtered and the methanol is removed using a reduced pressure concentrator to obtain the black ginseng extract of the present invention.

The black ginseng extract prepared by the manufacturing method is dried through a drying method common in the art such as shade drying, freeze drying, and then pulverized and powdered into fine particles, preferably a particle size of about 50 μm to 200 μm. Through the use of excipients or carriers well known in the art can be prepared in compositions such as pills, capsules, tablets.

The black ginseng prepared by the above method may be used as a raw material for various preparations by grinding into fine powder, preferably powder of about 50 to 200 μm, using a herbal medicine grinder.

The present invention can be applied to ginseng leaves as well as ginseng itself. Although ginseng leaves have not been used for medicinal purposes, they have been discarded or used for feed, and some of them have been used as raw materials for cosmetics or foods. However, when ginseng leaf extracts are heated and pressurized in the same way as in the present invention, the medicinal effect is much better. Since it is fortified, it can be used for medicinal purposes as well as the ever-used ginseng leaves.

The black ginseng prepared by the above method is characterized in that the existing ginseng, white ginseng, or red ginseng does not exist or enhances the content of the active ingredient present in trace amounts.

Black ginseng extract prepared by the production method of the present invention is characterized by showing a stronger antioxidant effect than conventional red ginseng.

Therefore, the present invention provides an antioxidant, an anti-aging pharmaceutical composition, and a health functional food containing the black ginseng extract prepared from the extraction method as an active ingredient.

The pharmaceutical composition containing the black ginseng extract of the present invention comprises 0.1 to 50% by weight of the black ginseng extract based on the total weight of the composition.

The pharmaceutical composition comprising the black ginseng extract of the present invention may further include suitable carriers, excipients and diluents commonly used in the preparation of pharmaceutical compositions.

Pharmaceutical compositions comprising black ginseng extract according to the present invention, respectively, in the form of powder, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, oral formulations, external preparations, suppositories, and sterile injectable solutions according to a conventional method. Can be formulated and used. Examples of carriers, excipients and diluents that can be included in the composition containing the extract include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate , Cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules and the like, and such solid preparations may include at least one excipient such as starch, calcium carbonate and sucrose in the extract. ) Or lactose, gelatin and the like are mixed. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The preferred dosage of the black ginseng extract of the present invention depends on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, for the preferred effect, the extract of the present invention is preferably administered at 0.2 to 200 mg / kg, preferably 2 to 100 mg / kg. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

Black ginseng extract of the present invention can be administered to various mammals such as mice, mice, livestock, humans. All modes of administration can be expected, for example by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injection.

Black ginseng extract of the present invention has little toxicity and side effects, so can be used with confidence even for long-term use for the purpose of prevention or treatment.

The present invention provides an anti-aging health functional food having antioxidant activity containing black ginseng extract showing an antioxidant effect as an active ingredient.

Examples of the food to which the black ginseng extract of the present invention can be added include various foods, beverages, gums, teas, vitamin complexes, and health functional foods.

It may also be added to foods or beverages for the purpose of antioxidant effects. At this time, the amount of the black ginseng extract in the food or beverage may be added in 0.01 to 15% by weight of the total food weight, the health beverage composition may be added in a ratio of 0.02 to 5g, preferably 0.3 to 1g based on 100ml have.

Food supplement additives as defined herein include food additives customary in the art, such as flavorings, flavors, colorants, fillers, stabilizers, and the like, and are exemplified below.

The health functional beverage composition of the present invention is not particularly limited to other ingredients except for containing the black ginseng extract as essential ingredients in the indicated ratios, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. . Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, black ginseng extract of the present invention is a variety of nutrients, vitamins, minerals (electrolyte), synthetic flavors and natural flavors, such as flavoring agents, coloring and neutralizing agents (such as cheese, chocolate), pectic acid and salts thereof, alginic acid and Salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks, and the like. In addition, the extract of the present invention may contain natural fruit juice and fruit flesh for the production of fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but is usually selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the extract of the present invention.

Hereinafter, the present invention will be described in detail with reference to the following examples and experimental examples.

However, the following Examples and Experimental Examples are only illustrative of the present invention, and the content of the present invention is not limited by the following Experimental Examples.

Comparative Example  1. Red Ginseng Methanol Extract and Butanol  Preparation of Extract

      20 g of red ginseng purchased from Kumsan Cheonnyeon Hongsam Co., Ltd. was pulverized, and 100 ml of water was added thereto and left at room temperature for 5 hours. Then, 1 liter of methanol was added and refluxed three times for 3 hours, and the extracted solution was cooled to room temperature. After filtering and removing methanol using a vacuum concentrator, 8.1 g of red ginseng methanol extract was obtained.

200 ml of water was suspended in 4.05 g of red ginseng methanol extract, and then 200 ml of ether was added to remove the nonpolar material. 200 ml of saturated butanol was added to the remaining aqueous layer to extract saponins present in ginseng, and then, butanol was removed by a vacuum condenser to obtain 0.65 g of butanol extract containing a large amount of ginseng saponin.

The red ginseng methanol extract and butanol extract was used as a comparative sample of the antioxidant experiment of Experimental Example 1.

Example  1. Preparation of Black Ginseng

      After purchasing five-year-old ginseng produced and distributed in Geumsan, the ginseng was put in a screw washer and washed three times in total for 10 minutes. At this time, care was taken not to peel during the washing process.

If the washed ginseng steamed without drying, the fresh ginseng is often bursting, so it was first dried at 40 ℃ for 24 hours to prevent this. The ginseng dried at low temperature for 24 hours was placed in Onggi (see FIG. 1) using a steamer, and primary steaming was performed at 95 ° C. for 6 hours except for the preheating time.

The preheating time is the time it takes for the steam to leak out from the pot and it usually takes about 30 minutes. After maintaining the internal temperature of the dryer with a built-in heating wire at 60 ℃, while drying the second ginseng evenly while rotating the fan for 12 hours, and then steamed for 2 hours at 115 ℃ for 2 hours, and then 3 times drying in the dryer for 12 hours To prepare a black ginseng of the present invention.

Example  2. Black Ginseng Methanol Extract and Butanol  Preparation of Extract

      20 g of black ginseng prepared by the method of Example 1 was taken and pulverized, 100 ml of water was added thereto, and the mixture was left at room temperature for 5 hours. Then, 1 L of methanol was added and the mixture was refluxed three times for 3 hours.

The extracted solution was cooled to room temperature, filtered, and methanol was removed using a vacuum condenser to obtain 7.4 g of black ginseng methanol extract.

200 g of water was added and suspended in 3.2 g of black ginseng extract, and then 200 ml of ether was added to remove the nonpolar material. 200 ml of saturated butanol was added to the remaining aqueous layer to extract saponins present in ginseng, and then butanol was removed using a vacuum condenser to obtain 0.7 g of butanol extract containing a large amount of ginseng saponin.

The black ginseng methanol extract and butanol extract was used as a sample of the component analysis of Example 3, the antioxidant experiment of Experimental Example 1 and the acute toxicity test of Experimental Example 2 below.

Example  3. Analysis of Black Ginseng Saponin Components

In order to analyze the components of ginseng saponin present in the black ginseng extract, 20 mg of the black ginseng butanol extract obtained in Example 2 was dissolved in 1 ml of methanol, and the filtrate was filtered through a 0.45 μm filter. It was. As a result of analyzing the ingredients, Rb1 2.3mg, Rb2 2.1mg, Rc 1.3mg, Rd 1.4mg, Re 0.5mg, Rg1 1.2mg, Rk1 3.7mg, Rg5 4.7mg, It was confirmed that 5.4 mg of Rg3 (S) and 4.5 mg of Rg3 (R) were present.

That is, it was confirmed that ginseng saponin existed at an excellent or similar level when compared with the black ginseng prepared using the existing augmentation width principle.

Flow rate 1ml / min Detector ELSD-LT detector Solvent A; CH ₃ CN: H ₂ O: 5% CH ₃ COOH = 15: 80: 5, B; CH ₃ CN: H ₂ O = 80:20
0 minutes (0% B), 0-10 minutes (30% B), 10-25 minutes (50% B), 25-40 (100% B), 40-50 minutes (100% B), 50-55 Minute (0% B), 55-58 minutes (0% B) Temperature 40 degrees

Experimental Example 1. Antioxidant Experiment

In order to measure the antioxidant activity of the black ginseng extract, a radical scavenging activity was performed on the red ginseng extract of Comparative Example 1 and the black ginseng extract of Example 2 using TOSC (Total Oxyradical Scavenging Capacity) assay.

The TOSC assay is proposed by Winston et al., Free Radic Biol Med., Feb, 24 (3), pp480-493, 1998 and modified by the same authors (Regoli and Winston, Toxicol Appl Pharmacol., Apr 15, 156 (2) , pp96-105, 1999).

Peroxyl radicals were generated by thermal homolysis of ABAP (2,2'-azobisamidinopropane) at 35 ° C (Winston et al., Free Radic Biol) Med ., Feb, 24 (3), pp480-493, 1998), and hydroxyl radicals are the Fenton reaction using Fe and ascorbate (Fenton reaction; Winston and Cederbaum, Alcholol Clin Exp Res ., 9 (2), pp95-102, 1985), peroxynitrite was generated through spontaneous decomposition of SIN-1. Each reactive oxygen species generated reacts with α-keto-γ-methiolbutyric acid (KMBA) to generate ethylene gas, which does not show any temperature-dependent difference. It has been reported (Winston et al., Free Radic Biol Med ., 24 (3), pp480-493, 1998; Regoli and Winston, Toxicol Appl Pharmacol. , 156 (2), pp96-105, 1999).

The reaction proceeded by putting the final volume of 1 ml of the reaction solution into a sealed 10 ml container with rubber septum. Ethylene gas generation detection takes a predetermined volume of air in the head space of the reaction vessel and is equipped with a gas chromatography equipped with a Poropack N column and a flame ionization detector (FID). Was analyzed.

As a result of the experiment, the area under the kinetic curve (AUC) was obtained by integrating a graph obtained from the experimental measurements, and the TOSC value was calculated through Equation 1 below (see Table 2 and Table 3).

TOSC = 100-(∫SA / ∫CA × 100)

∫SA = range integrated from the curve of the sample response

(integrated area from the curve of the sample reaction)

∫CA = integrated range from curve of control response

(integrated area from the curve of the control reaction)

For samples that do not have any oxyradical scavenging capacity, ∫SA / ∫CA = 1 and have a value of TOSC = 0. Conversely, when ∫SA → 0, the TOSC value approaches 100. The value of TOSC is compared with the value in the control, so it is theoretically unaffected by the sensitivity of the instrument, reagents used, or other reaction conditions.

GC equipped with Poropack N column and FID (flame ionizing detector) for the detection of ethylene gas was placed in an oven at 60 ° C, injector 180 ° C and detector 180. It was fixed at 캜 and helium was injected into the column at a rate of 30 ml / min as the mobile phase. For the determination of the ethylene gas in the vial, 150 μl of air in the vial was taken with a gas-tight syringe using the head-space technique, and then gas-tight. The test was performed by injecting a syringe into an injector.

Total Oxyradical Scavenging Ability for Peroxyl Radicals
{Total oxy-radical scavenging capacity (TOSC) value against peroxyl radiacls} Antioxidant
(Antioxidant) TOSC value according to concentration Specific TOSC Value (TOSC / mg) Black ginseng
Butanol extract Concentration (mg / ml) 0.025 0.05 0.1 0.2 476 ± 91 TOSC value 8.10 32.35 45.33 51.80 Black ginseng
Methanol extract Concentration (mM) 0.025 0.05 0.1 0.2 433 ± 160 TOSC value 28.25 40.92 47.00 64.46 Red ginseng
Butanol extract Concentration (mM) 0.025 0.05 0.1 0.2 423 ± 122 TOSC value 3.69 31.27 38.87 41.30 Red ginseng
Methanol extract Concentration (mM) 0.025 0.05 0.1 0.2 453 ± 88
TOSC value 17.08 34.13 45.98 53.00

Total Oxyradical Scavenging Ability for Peroxidation
{Total oxy-radical scavenging capacity (TOSC) value against peroxynitrite} Antioxidant
(Antioxidant) TOSC value according to concentration Specific TOSC Value (TOSC / mg) Black ginseng
Butanol extract Concentration (mg / ml) 0.025 0.05 0.1 0.2 405 ± 39 TOSC value 6.48 22.19 39.07 46.13 Black ginseng
Methanol extract Concentration (mM) 0.025 0.05 0.1 0.2 355 ± 34 TOSC value 7.65 21.11 34.73 40.70 Red ginseng
Butanol extract Concentration (mM) 0.025 0.05 0.1 0.2 232 ± 24 TOSC value 4.10 13.33 22.48 31.84 Red ginseng
Methanol extract Concentration (mM) 0.025 0.05 0.1 0.2 204 ± 9 TOSC value 3.94 10.19 20.00 25.38

As a result of the experiment, red ginseng and black ginseng showed high antioxidant activity against peroxyl radicals and peroxynitrite at 0.2 mg / ml or less.

Scavenging of peroxyl radicals was observed to be comparable to red and black ginseng (see Table 2).

However, the scavenging ability of peroxynitrite showed 1.7 times higher activity of black ginseng butanol extract and methanol extract than red ginseng butanol extract and methanol extract (see Table 3).

These experimental results indicate that the type or ratio of the composition may change depending on the method of aging ginseng, which may cause a difference in the antioxidant effect.

Experimental Example  2. Acute Toxicity Test

Acute toxicity test was performed using 6-week-old specific pathogen-free (SPF) SD rats supplied from the Korean Experimental Animal Center as follows.

Two animals of each group were orally administered to the black ginseng methanol extract and black ginseng butanol extract of Example 2 at a dose of 1 g / kg.

After administration of the test substance, mortality, clinical symptoms, and body weight changes were observed, and hematological and hematological examinations were performed.

As a result, no clinical symptoms or dead animals were found in all animals treated with the test substance, and no toxic changes were observed in weight changes, blood tests, blood biochemical tests, and autopsy findings.

As a result, the extract of the present invention did not show a change in toxicity even in rats up to 1 g / kg, respectively, the minimum lethal dose (LD ₅₀ ) was determined to be a safe substance of more than 1 g / kg.

The preparation example of the pharmaceutical composition comprising the black ginseng extract of the present invention will be described, but the present invention is not intended to be limited thereto, but is intended to be described in detail.

Formulation Example 1 Preparation of Powder

Black Ginseng Extract 300 mg

Lactose 100 mg

Talc 10 mg

The above ingredients are mixed and filled in an airtight cloth to prepare a powder.

Formulation Example 2 Preparation of Tablet

Black ginseng extract 50 mg

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, tablets are prepared by tableting according to a conventional method for preparing tablets.

Formulation example  3. Manufacture of capsule

Black ginseng extract 50 mg

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

According to a conventional capsule preparation method, the above ingredients are mixed and filled into gelatin capsules to prepare capsules.

Formulation example  4. Preparation of injections

Black ginseng extract 50 mg

Sterile sterilized water for injection

pH adjuster

According to the conventional method for preparing an injection, the amount of the above ingredient is prepared per ampoule (2 ml).

Formulation example  5. Liquid  Produce

Black Ginseng Extract 100 mg

10 g of isomerized sugar

5 g of mannitol

Purified water

After dissolving each component in purified water according to the usual method of preparing a liquid solution, adding lemon flavor appropriately, mixing the above components, adding purified water, adjusting the whole to 100 ml by adding purified water, and then filling into a brown bottle. The solution is prepared by sterilization.

Formulation example  6. Manufacture of health food

Black Ginseng Extract 1000mg

Vitamin mixture proper amount

70 μg of Vitamin A Acetate

Vitamin E 1.0 mg

0.13 mg of vitamin B ₁

0.15 mg of vitamin B ₂

0.5 mg of vitamin B ₆

Vitamin B ₁₂ 0.2 g

Vitamin C 10 mg

10 μg biotin

Nicotinic Acid 1.7 mg

50 μg folic acid

Calcium Pantothenate 0.5mg

Mineral mixture

Ferrous Sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium Citrate 90 mg

Calcium Carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

Formulation example  7. Manufacture of health drinks

Black Ginseng Extract 1000mg

Citric acid 1000 mg

100 g oligosaccharides

Plum concentrate 2 g

1 g of taurine

Add 900 ml of purified water

After mixing the above components according to a conventional healthy beverage production method, and then stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and refrigerated and then stored in the present invention For the preparation of healthy beverage compositions.

Although the compositional ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the compounding ratio according to the regional or national preference such as the demand class, the demanding country, and the use purpose.

As described above, the present invention relates to a composition containing a black ginseng extract obtained from black ginseng prepared using ginseng, the composition shows a strong antioxidant effect can be useful as an anti-aging pharmaceutical composition and health functional food .

Claims (5)

delete delete delete delete Washing the fresh ginseng for 4 to 6 years and drying it for 20 to 28 hours at a temperature of 35 to 55 ° C .; Primary steaming step of steaming for 5 to 7 hours at a temperature of 85 to 105 ℃ in Onggi; Secondary drying step of drying for 9 to 15 hours at a temperature of 50 to 70 ℃; Secondary steaming step of steaming for 5 to 7 hours at a temperature of 95 to 125 ℃; Obtaining black ginseng, comprising a third drying step of drying for 12 hours; An antioxidant containing black ginseng butanol extract obtained by pulverizing it, adding 4-6 times the mass of black ginseng to water for 3 to 7 hours, and extracting the black ginseng extract extracted with methanol or the extract extracted with methanol again with butanol as an active ingredient. Antioxidant with activity.

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