KR20110008648A - Skin care mask using hydrogel containing arazyme - Google Patents

Abstract

PURPOSE: A skin care mask using hydrogel containing arazyme is provided to ensure enzyme stability and to continuously transfer arazyme efficiency. CONSTITUTION: A skin care mask of hydrogel contains arazyme. The hydrogel contains polymer and cross-linking agent. The polymer contains 2.0-4.0 weight parts of sodium polyacrylate. The cross-linking agent contains 2.0-4.0 weight parts of aluminum chloride. The hydrogel further contains purified water, glycerin, chondrus crispus, PEG-60 hydrogenated castor oil, potassium hydroxide and sodium hyaluronate. A method for manufacturing the mask comprises: a step of mixing arazyme, synthetic polymer and cross-linking agent and stirring to obtain hydrogel; and a step of making the hydrogel in a mask form.

Description

Skin care mask using hydrogel containing arazyme

The present invention relates to a skin care mask using a hydrogel containing arazyme.

The interest in women's skin care has continued, and modern times have entered the aging society, and especially today the concept of wellbeing has emerged as the best sociocultural topic for a happy and healthy life. There is a growing desire to maintain skin continuously. In line with this, a lot of cosmetics have been developed and the skin care related industry is developing. The buzzword of today's cosmetics development is by far the efficient delivery of cosmetic ingredients to the skin. To this end, various studies are being conducted on nanotechnology, liposomes, electrical enhancers, and chemical enhancers. New cosmetics are being developed through research, and popular products are moisturizing, nutritious and easy to use. Among these, mask packs and sheet products useful for skin care are stabilized and delivered for more effective and intensive stabilization and delivery of cosmetic ingredients, and patch-type formulations have been studied for delivery of nutrients.

Skin care mask is a product that attaches a substance to the skin to make it clean and beautiful, and then removes or rinses it after a certain time. It can be used for the whole body as well as the face.

Skincare masks are essence, dry and gel type masks depending on the formulation, and gel masks are classified into water-soluble gel masks and insoluble gel masks. The classification of the skin care mask is shown in [Table 1].

1st brother Statue Nonwoven type (essence type) Technology that wet functional latex and lotion on nonwoven fabric Dry type Technology containing active ingredient in adhesive such as freeze-dried collagen sheet Gel type
(gel type) receptivity
(hydrogel) Technology to enclose active ingredient in water-soluble hydrogel Insoluble
(insoluble gel) Technology to enclose active ingredients in insoluble gels

Hydrogels prepared through gelling of polymeric materials have been studied as drug delivery systems (DDS) to control and sustain the penetration of cosmetic and pharmacological components. The drug delivery system is a technology that optimizes drug treatment and design formulations to effectively reduce the side effects of drugs, improve patient compliance with drugs, and maximize the efficacy and effects by efficiently delivering drugs for treating diseases to the treatment site. Is generically. In particular, the transdermal delivery system (TDS), which delivers drugs through the skin among the drug delivery systems, is the most similar technology to the skincare hydro mask, so it includes effective cosmetic ingredients instead of drugs in the mask formulation and is applied for skincare. This is possible.

Hydrogel is a 3D mesh insoluble material that physically chemically cross-links a homopolymer or copolymer of a water-soluble polymer, and has a swelling property to absorb fluid from the outside. As a composition of Topical Dosage Foam, which contains a large amount of moisture or biological fluid as a tissue, is softer than other synthetic biological materials, and has excellent biocompatibility, it has recently been in the spotlight and has been studied as a formulation having various characteristics.

Hydrogels have the property of adsorbing and releasing large amounts of solvent. In addition, there is the ability to encapsulate and stabilize the material to be contained according to the pore size (capture size) to contain a functional material and the like. In addition, various studies have been reported one after another due to changes in the characteristics such as pore size, expansion and hydrophilicity, hydrophobicity depending on physical and chemical conditions such as temperature, pH, ionic strength, light.

Skin care hydrogel mask using the hydrogel is a product that combines medical technology with a simple cosmetic mask. By storing the active ingredient inside the water-soluble polymer in the hydrogel can be seen as a skin care delivery system (system) designed to continuously and effectively deliver the efficacy and effect of the active ingredient. In general, the polymer material used in the hydrogel is a natural polymer and a synthetic polymer as shown in Table 2 below.

division Used polymer natural animal Gelatin, Casein, Albumin plant Starch Gum Arabic, Locust Bean Gum, Xanthan Gum, Tragacanth, Karpectine Alginate, Carrageenan, Agar Semi-synthetic cellulose
derivative
(Cellulose
Derivatives) Carboxy Methyl Cellulose (CMC), Methyl Cellulose (MC), Ethyl Cellulose (EC), Hydroxyethyl Cellulose (HEC), Cellulasetate Phthalate (CAP), Stearyl Cellulose Starch derivative
(Starch
Derivatives) Starch Phosphate, Cyano Ethylated Starch, Carboxy Methyl Starch (CMS), Hydroxy Ethylated Starch synthesis acid Polyacrylic Acid, Polymethylacrylic Acid, Polystyrene Sulfonic Acid, Styrene-maleic Anhydride Copolymer Basic Polyethylene Imine, Polyvinyl Pyridine, Polydiethylamino Ethylacrylate neutrality Salts of above acidics & basics Non-ion Polyvinyl Alcohol (PVA), Polyacrylamide (PAAm), Alkyds, Polyvinyl Pyrrolidone (PVP), Polyethylene Glycol, Polyethylene Polyvinyl Methyl Ether Polyvinyl Methyl Ether)

Hydrogel is a very important ingredient that contains cosmetic ingredients and should have proper strength, uniformity and adhesiveness, and it should have the basic property of not sticking to the skin when peeled off. In general, hydrogels exhibit various characteristics depending on the selection and content of the polymer, crosslinking agent, large amount of water, plasticizer and auxiliary polymer to be used.

Hydrogels form a gel by crosslinking polyvalent metals between molecules of anionic acrylic polymers. Such chemical reactions vary in speed, reaction form, and strength of crosslinking reactions depending on the solubility of the polyvalent metal salt as a crosslinking agent. The choice of the appropriate polyvalent metal salt is important. In the case of using a water-soluble polyvalent metal salt as a crosslinking agent, the crosslinking reaction proceeds very rapidly at the contact interface between the polymer and the crosslinking agent, resulting in agglomeration of the gel, resulting in an uneven gel, and when a poorly soluble polyvalent metal salt is used. It may be easy to obtain a homogeneous hydrogel, but the rate of crosslinking reaction may be too slow, so that the gel may not be easily formed or diarrhea may occur. Due to this problem, in the case of using a water-soluble polyvalent metal salt and a poorly soluble polyvalent metal salt, it is possible to obtain a uniform hydrogel by controlling the dissociation rate of the polyvalent metal salt.

Arazyme (arayzme) is a protease and exhibits stable proteolytic activity at low temperatures and high salt concentrations, as well as the highest activity at human body temperature of 37 ℃ and stable activity over a wide pH range. In addition to proteolytic function, arazyme is known to have high antibiotic and antibacterial activity against various pathogenic microorganisms. Due to its strong antibacterial and antimicrobial effects, it can be fed to livestock as a disease prevention or growth accelerator. In addition, breast cancer and lung cancer inhibitory activity and liver function protection activity of arazyme have been reported. Arazyme's high-efficiency proteolytic function exerts an excellent exfoliating effect, making it easy to exfoliate the dead skin, face and body instead of chemicals.

Therefore, the inventors of the present invention are to study the hydrogel containing arazyme while researching to enhance the efficacy and effect of useful ingredients on the skin at the same time to promote the delivery of the skin by containing a functional natural cosmetic ingredients using the hydrogel system. The used skincare mask is evenly distributed in the mask, has enzyme stability, and is useful as a skincare formulation that continuously delivers the effects of arazyme by having protein resolution through recovery of arazyme activity by immobilization. The present invention has been completed by confirming that it can be used.

It is an object of the present invention to provide a skin care mask using a hydrogel containing arazyme.

In order to achieve the above object, the present invention provides a skin care hydrogel mask containing arazyme.

The present invention also provides a method for producing a skin care hydrogel mask containing arazyme.

The skin care mask using the hydrogel containing arazyme of the present invention has the arazyme evenly distributed on the mask and has enzymatic stability compared to the unimmobilized arazyme solution, and the protein resolution through restoring arazyme activity by immobilization. As well as the hydrogel containing liposomes arazyme has stability over time, it can be usefully used as a formulation for skin care to continuously deliver the efficacy of arazyme.

Hereinafter, the present invention will be described in detail.

The present invention provides a skin care hydrogel mask containing arazyme.

The arazyme is

1) a protein comprising the amino acid sequence set forth in SEQ ID NO: 1;

2) a protein encoded by a DNA (DNA) comprising a coding region of the nucleotide sequence set forth in SEQ ID NO: 2;

3) a protein consisting of an amino acid sequence in which one or more amino acids in the amino acid sequence set forth in SEQ ID NO: 1 is substituted, deleted, inserted and / or added, and which is functionally identical to the protein comprising the amino acid sequence set forth in SEQ ID NO: 1; And

4) a protein encoded by a DNA that hybridizes under stringent conditions to a DNA comprising the nucleotide sequence of SEQ ID NO: 2, and functionally identical to a protein comprising the amino acid sequence of SEQ ID NO: 1 It is preferably any one of but is not limited thereto.

If the hybridization is performed under stringent conditions, the DNA having a high homology sequence is selected and the resulting protein is likely to contain a protein functionally identical to arazyme. A highly homologous nucleotide sequence means, for example, a sequence having at least 70%, preferably at least 80%, more preferably at least 90%, most preferably at least 95% identity with the nucleotide sequence set forth in SEQ ID NO: 2. do. The amino acid sequence may be a high homology sequence of 70% or more, preferably 80% or more, more preferably 90% or more, most preferably 95% or more with the amino acid sequence of SEQ ID NO: 1. The homology ratios above can be determined by common algorithms selected by those skilled in the art. The hybridization is provided by DNA-DNA hybridization under stringent conditions well known in the art (Hames and Higgins, Eds. Nucleic Acid Hybridisation , IRL Press, UK, 1985).

The hydrogel preferably uses a polymer and a crosslinking agent, but is not limited thereto.

The polymer preferably contains 2.0 to 4.0 parts by weight of sodium polyacrylate, and more preferably 2.5 to 3.5 parts by weight, but is not limited thereto.

The crosslinking agent preferably contains 2.0 to 4.0 parts by weight of aluminum chloride, which is a water-soluble polyvalent metal salt, and more preferably 2.5 to 3.5 parts by weight, but is not limited thereto.

Appropriate selection is important because the characteristics of the resulting gel are formed very differently depending on the choice of the polymer material used as the skeleton of the gel structure used in the hydrogel of the present invention and the polyvalent metal salt used as the crosslinking agent. The polymer material is gelatin, casein, albumin, starch, gum arabic, locust bean gum, xanthan, which are natural polymers commonly used in hydrogels. Xanthan gum, tragacanth, karpectine, alginate, carrageenan and agar can be used and carboxyl methyl, a semisynthetic polymer, can be used. cellulose, CMC), methyl cellulose (MC), ethyl cellulose (EC), hydroxethyl cellulose (HEC), celluacetate phthalate (CAP), stearyl cellulose ), Starch phosphate, cyano ethylated starch, carboxy methyl starch (CMS) and hydroxy ethylated starch can be used. The synthetic polymers include polymethylacrylic acid, polystyrene sulfonic acid, styrene-maleic anhydride copolymer, polyethylene imine, and polyvinyl pyridine. polyvinyl pyridine, polydiethylamino ethylacrylate, polyvinyl alcohol (PVA), polyacrylamide (PAAm), alkyds, polyvinyl pyrrolidone, polyethylene Polyethylene glycol and polyethylene polyvinyl methyl ether may be used.

The hydrogel may be purified water, glycerin, poor poverty, chondrus crispus, PIG-60 hydrogenated castor oil, dipotassium glycyrrhizate, methyl Paraben (methylparaben), propylparaben (propylparaben), disodium EDTA (diisodium EDTA), potassium hydroxide (potassium hydroxide) and sodium hyaluronate (sodium hyaluronate) at least one selected from the group consisting of Preferably, but not limited to.

The hydrogel is protease, plant extract, water-soluble collagen (hydrolyzed collagen), ubiquinone, ubiquinone, tocopheryl acetate, arbutin, colloidal platinum enzyme, ceratiopeptide It is preferable to further include one or more selected from the group consisting of lipase, cellulase and xylanase, but is not limited thereto.

The protease may be a plant protease such as papain, bromelain, animal protease such as trypsin, and protease obtained through microbial culture such as Bacillus and Aspergillus.

The plant extract is portulaca oleracea extract, arnica montana flower extract, gentiana lutea root extract, amaryllis millefolium extract, wormwood extract (artemisia vulgaris extract) and chemomile Chamomilla recutita flower extract can be used.

The present inventors prescribed the sodium polyacrylate and aluminum chloride content under various conditions (see Table 3) to determine the proper content of the polymer and the crosslinking agent used in the hydrogel to measure the properties of the hydrogel. As a result, as the content of sodium polyacrylate was increased, the hydrogel was formed in a homogeneous phase. As the content of aluminum chloride was increased, the viscosity was increased to form a uniform hydrogel. As a result, the physical properties of the hydrogel using 3 parts by weight of sodium polyacrylate and 3 parts by weight of aluminum chloride showed the most optimal state (see Table 4).

The present inventors aged under each temperature condition to determine the degree of curing of the hydrogel according to the time and temperature change of the crosslinking reaction of the hydrogel, and performed the naked eye evaluation, thermal stability evaluation and underwater swelling evaluation of the hydrogel. . As a result, as the temperature was increased, the degree of curing was faster (see Table 6). As the crosslinking reaction proceeded, the heat resistance was improved (see Table 8), and as the swelling ratio was increased, the crosslinking reaction was confirmed.

The present inventors cut the five parts of the hydrogel (see FIG. 1) and placed them on a skim milk plate in order to examine the even distribution and immobilization of the enzyme in the hydrogel containing arazyme. As a result, there was no difference in activity of each site of the hydrogel (see FIG. 2). Thus, an even distribution of arazyme fixed to the hydrogel was confirmed.

The present inventors placed the two samples on a skim milk plate and compared the activity of the arazym immobilized on the hydrogel with the non-immobilized arazyme solution. As a result, when left at 60 ° C., the arazyme solution did not show activity, but the arazyme immobilized on the hydrogel showed a late activity (see FIG. 3). As a result, it was confirmed that the arazyme was not only stably immobilized by hydrogel but also had thermal stability due to immobilization.

The present inventors looked at the change in activity through standing for a certain time at 60 ℃ to determine the stability through the recovery of activity of the arazy immobilized on the hydrogel. As a result, the longer the recovery time, the longer it takes to recover the activity, it was confirmed that the degradation of the protein or keratin through recovery in the range in which the arazyme activity was not lost by immobilization of the hydrogel (see FIG. 4).

The present inventors carried out physical property and sensory evaluation to confirm the adhesive force of the hydrogel containing a non-ionic surfactant. As a result, the adhesive strength was higher than that of the hydrogel added with KM205C or ZKS-1670 (see Table 10), and the hydrogel added with KM205C showed stronger adhesiveness (see Table 12). .

The present inventors performed the hydrogel properties, odors, pH and microbial evaluation under severe conditions in order to confirm the stability of the hydrogel containing liposome-ized arazyme and cosmetic ingredients over time. As a result, there was no change in the hydrogel and it appeared to meet the microbial acceptance criteria (100 cfu / ml) (see Table 13).

The present inventors measured the moisture content of the hydrogel containing the moisturizing component and the moisturizing component in order to measure the moisturizing effect of the moisturizing component (glycerine, 1,3-butylene glycol, sodium hyaluronate, sorbitol). As a result, the water retention ability was the best sodium hyaluronate, hydrogel containing a moisturizing component showed an excellent moisturizing effect overall. In particular, the hydrogel containing sodium hyaluronate was excellent moisturizing effect. When hyaluronic acid sodium is applied to anti-wrinkle hydrogels, excellent moisturizing effect can be expected.

Therefore, the hydrogel containing arazyme showed even distribution of arazyme and increased stability, which may play a sufficient role of the enzyme, and it was confirmed that the hydrogel containing liposome-ized arazyme has stability over time, so that the skin It can be seen that it can be usefully used as a care hydrogel mask.

In addition,

1) preparing a hydrogel by mixing and stirring arazyme, synthetic polymer and crosslinking agent; And

2) provides a method for manufacturing a skin care hydrogel mask comprising the step of preparing the hydrogel in the form of a mask.

In the method, the arazyme of step 1) is

1) a protein comprising the amino acid sequence set forth in SEQ ID NO: 1;

2) a protein encoded by a DNA (DNA) comprising a coding region of the nucleotide sequence set forth in SEQ ID NO: 2;

3) a protein consisting of an amino acid sequence in which one or more amino acids in the amino acid sequence set forth in SEQ ID NO: 1 is substituted, deleted, inserted and / or added, and which is functionally identical to the protein comprising the amino acid sequence set forth in SEQ ID NO: 1; And

4) a protein encoded by a DNA that hybridizes under stringent conditions to a DNA comprising the nucleotide sequence of SEQ ID NO: 2, and functionally identical to a protein comprising the amino acid sequence of SEQ ID NO: 1 It is preferably any one of but is not limited thereto.

If the hybridization is performed under stringent conditions, the DNA having a high homology sequence is selected and the resulting protein is likely to contain a protein functionally identical to arazyme. A highly homologous nucleotide sequence means, for example, a sequence having at least 70%, preferably at least 80%, more preferably at least 90%, most preferably at least 95% identity with the nucleotide sequence set forth in SEQ ID NO: 2. do. The amino acid sequence may be a high homology sequence of 70% or more, preferably 80% or more, more preferably 90% or more, most preferably 95% or more with the amino acid sequence of SEQ ID NO: 1. The homology ratios above can be determined by common algorithms selected by those skilled in the art. The hybridization is provided by DNA-DNA hybridization under stringent conditions well known in the art (Hames and Higgins, Eds. Nucleic Acid Hybridisation , IRL Press, UK, 1985).

The polymer preferably contains 2.0 to 4.0 parts by weight of sodium polyacrylate, and more preferably 2.5 to 3.5 parts by weight, but is not limited thereto.

The crosslinking agent preferably contains 2.0 to 4.0 parts by weight of aluminum chloride, which is a water-soluble polyvalent metal salt, and more preferably 2.5 to 3.5 parts by weight, but is not limited thereto.

The hydrogels are proteolytic enzymes, plant extracts and hydrolyzed collagen, ubiquinone, tocopheryl acetate, arbutin, colloidal platinum enzyme, ceratiopeptides It is preferable to further include one or more selected from the group consisting of lipase, cellulase and xylanase, but is not limited thereto.

The present inventors used polyacrylic acid, which is an acidic synthetic polymer, as a polymer material used in the preparation of a hydrogel, and a crosslinking agent used aluminum chloride, which is a water-soluble polyvalent metal salt. The hydrogel material was mixed and stirred uniformly at room temperature. At this time, the powder raw material was deposited so as not to generate bubbles, and then stirred and homogenized, and then mixed well for about 30 minutes to prevent bubbles from being produced using a disper mixer to prepare a uniform gel. The gel base uniformly prepared without bubbles was coated with a thickness of 1 mm on a polypropylene film on which one side was release-treated with silicone. After rolling, the polyester nonwoven fabric having a basis weight of 100 g / m ² was flatly attached and the surface of the nonwoven fabric was flattened using a roller. The hydrogel thus produced was cut into a certain size and placed in a cosmetic aluminum pouch to prevent evaporation of moisture, and then packaged and stored in a heat sealer.

The present invention is a skin care hydrogel mask containing arazyme is evenly distributed in the arazyme mask and has an enzyme stability compared to the unimmobilized arazyme solution, and has a protein degradation ability through recovery of arazyme activity by immobilization In addition, it can be seen that the hydrogel containing liposome-formed arazyme can be used for the preparation of a skin care hydrogel mask that continuously delivers the efficacy of arazyme by confirming that it has stability over time.

Hereinafter, the present invention will be described in detail by Examples and Experimental Examples.

However, the following Examples and Experimental Examples specifically illustrate the present invention, and the content of the present invention is not limited by the Examples and Experimental Examples.

< Example  1> Hydrogel  Produce

The present inventors prepared a hydrogel having a composition of the following [Table 3]. Polyacrylic acid, an acidic synthetic polymer, was used as the polymer used in the preparation of the hydrogel, and aluminum chloride was used in a water-soluble polyvalent metal salt as a crosslinking agent. The a and b phases of Table 3 were mixed, respectively, and uniformly mixed and stirred at room temperature. At this time, the powder raw material was deposited and gradually stirred to homogenize so as not to generate bubbles. Then, while gradually adding phase b to phase a, a well-mixed gel was prepared by mixing well for about 30 minutes so that no bubbles were formed using a disper mixer. The gel base uniformly prepared without bubbles was coated with a thickness of 1 mm on a polypropylene film on which one side was release-treated with silicone. At this time, Dr. Braid manufactured by Hanyang Machinery Co., Ltd. was used for the leading edge of uniform thickness. After spreading, a polyester nonwoven fabric having a basis weight of 100 g / m ² was flatly attached, and the surface of the nonwoven fabric was also flattened using a roller manufactured by Hanyang Machinery Co., Ltd. The hydrogel thus produced was cut into a certain size and placed in a cosmetic aluminum pouch to prevent evaporation of moisture, and then packaged and stored in a heat sealer.

Prize ingredient A type Type B C type D type E type a Glycerin 20.0 20.0 20.0 20.0 20.0 Phenonip 0.3 0.3 0.3 0.3 0.3 Sodium polyacrylate
[sodium polyacrylate (MW: 4,500 ~ 5,000)] - 2.0 3.0 4.0 4.0 Aluminum chloride
AlCl ₃ (10% Soln. Aq.) 3.0 3.0 3.0 3.0 4.0 Sodium Carboxymethyl Cellulose (Na-CMC) 1.0 1.0 1.0 1.0 1.0 b Polyacrylic acid
[polyacrylic acid (MW: 250,000 ~ 300,000)] 6.0 6.0 6.0 6.0 6.0 D.I.Water 69.7 67.7 66.7 65.7 64.7 Total amount 100.0 100.0 100.0 100.0 100.0

< Experimental Example  1> Hydrogel  Property measurement

In order to determine the physical properties according to the content of the polymer and the crosslinking agent of the hydrogel prepared according to the <Example 1>, the viscosity of the hydrogel, the uniformity of the gel surface, the strength and pH of the gel was measured. The measurement of pH was measured by exposing the pH meter to the electrode and in direct contact with the hydrogel surface.

As a result, as shown in [Table 4], in the case of type A, the viscosity of the gel rose sharply from the beginning of stirring, which was not suitable for producing a uniform hydrogel, and a non-uniform hydrogel was formed in the type B. As the content of sodium polyacrylate was increased in the same amount of crosslinking agent (AlCl ₃ ), the viscosity of the gel was lowered and the pH and strength were increased to form a uniform hydrogel. On the contrary, when the content of AlCl ₃ , which is a crosslinking agent, is increased in a certain amount of sodium polyacrylate, the viscosity of the gel is rapidly increased to obtain a uniform hydrogel (Table 4). Thus, it was found that the most suitable physical property evaluation among the basic prescriptions was C type using 3 parts by weight of sodium polyacrylate and aluminum chloride, respectively.

type A type Type B C type D type E type Viscosity of gel Sharp rise height moderation moderation height Exterior - fitness Great Great Bad pH 2.6 4.2 4.5 4.7 4.6

< Experimental Example  2> Hydrogel  Hardness rating

<2-1> Visual evaluation

In order to determine the degree of curing according to the crosslinking reaction of the hydrogel of the present invention, using the C-type hydrogel, after aging at each temperature condition (5, 25 and 40 ℃), hydrogel visual evaluation was performed. According to visual evaluation criteria as shown in Table 5, when the coated hydrogel was pressed by hand, the state of the hydrogel was observed and evaluated.

As a result, as shown in [Table 6], the gel curing degree of the hydrogel according to the time and temperature change (5, 25 and 40 ℃) for the crosslinking reaction was confirmed that the time required for curing decreases with increasing temperature (Table 6).

Visual Evaluation Criteria for Evaluating Curing of Hydrogels Curing degree Evaluation standard One Hydrogels collapse and sticky 2 Hydrogel collapses slightly, slightly sticky 3 Hydrogel does not collapse, but slightly sticky 4 Hydrogels do not collapse, are elastic, non-sticky and do not get on hands

Curing degree of hydrogel with time and temperature Hour 0 6 12 24 48 60 72 96 120 140 180 circa
anger
Degree 5 ℃ One One One One 2 2 2 3 3 3 3 25 ℃ One One One 2 3 3 3 4 4 4 4 40 ℃ One One 2 3 3 4 4 4 4 4 4

<2-2> Heat stability  evaluation

In order to determine the degree of curing according to the crosslinking reaction of the hydrogel of the present invention, using the C-type hydrogel, after aging at each temperature condition (5, 25 and 40 ℃), the thermal stability evaluation of the hydrogel [ Table 7 was carried out according to the evaluation criteria.

In order to check the heat resistance of the hydrogel, the gel was cut into a size of 50 × 50 (mm) and contacted with a glass wall of a 500 ml beaker containing deionized water of 95 ° C. or more. Check the presence or absence. In addition, the sample was placed in a 500 ml beaker containing deionized water of 95 ° C. or higher, and deposited for 3 minutes, and then taken out to observe the sample to check the state of the hydrogel.

As a result, as shown in [Table 8], as the crosslinking reaction proceeded, it was confirmed that the heat resistance of the hydrogel was also improved (Table 8).

Thermal Stability Evaluation Criteria for Evaluating Curing of Hydrogels Heat resistance Evaluation standard One Gel sticks to glass beaker wall. Soak for 2 minutes to loosen and disperse the gel. 2 Does not adhere to glass beaker walls. After soaking for 2 minutes, the gel will drip like grass. After soaking for 2 minutes, the gel will appear on the wall. 3 No gel on the glass beaker wall. Continues to expand without sagging even after soaking for more than 1 hour.

Measurement of thermal stability of hydrogels over time and temperature Hour 0 6 12 24 48 60 72 96 120 140 180 of mine
Heat
castle 5 ℃ One One One One One 2 2 2 2 2 2 25 ℃ One One One 2 2 2 2 3 3 3 3 40 ℃ One One 2 2 2 3 3 3 3 3 3

<2-3> underwater Swelling ( Swelling ) evaluation

In order to determine the degree of curing according to the cross-linking reaction of the hydrogel of the present invention, using the C-type hydrogel, after aging at each temperature condition (5, 25 and 40 ℃), the swelling evaluation of the hydrogel in water Was carried out.

Hydrogels aged under each temperature condition were taken out for each time zone, cut into gels of 50 × 50 (mm) size, immersed in deionized water at 25 ° C., and taken out for evaluation. Deposition was carried out by dipping for 3 hours in a 500 ml beaker containing deionized water and immersing for 1 hour. Then, the sample was dried for 10 minutes at room temperature so that the free water on the surface of the sample was removed. Evaluated.

Swelling Ratio (%) = Wt-Wr / Wr × 100

Here, Wr is the initial weight of the sample before immersion in water and Wt is the weight of the sample after immersion in water and dried for 10 minutes.

As a result, when the degree of crosslinking was low, the hydrogel was released within 1 hour and dispersed in water to find the shape of the hydrogel. As time goes by, the expansion time of the hydrogel becomes longer and when the crosslinking time is reached, the hydrogel continues to expand for 24 hours. This loosening and dispersion in water was not seen. In the case of hydrogel aged at 5 ° C, swelling was not performed before 48 hours, and the shape of the hydrogel was dispersed in water during the test, and its shape was not found.In the case of hydrogel patches aged at 25 ° C, before 24 hours There was no swelling, and during the test, the hydrogel portion was dispersed in water and its shape could not be found. In addition, the hydrogel patch aged at 40 ° C. was not swelled before 12 hours, and the hydrogel portion was dispersed in water during the test, and thus the shape thereof was not found. It was confirmed that the time required for crosslinking was shortened according to the temperature at which aging was performed. As the swelling ratio was increased, the crosslinking reaction was performed.

< Experimental Example  3> In hydrogel Arazyme  Check distribution

Cut the 5 pieces from the different parts of the hydrogel made by containing arazyme as shown in Figure 1 and placed on a skim milk plate and left for 3, 6 and 67 hours at 26 ℃, the presence or absence of arazyme activity was measured .

As a result, as shown in [FIG. 2], there was no difference in the comparative activity for each part of the mask (FIG. 2). Thus, it was confirmed that arazyme is evenly distributed in the hydrogel.

< Experimental Example  4> In hydrogel Arazyme  Check stability

The non-immobilized arazyme stock solution and the arazyme immobilized on a hydrogel were placed on a skim milk plate and allowed to stand at 25, 37, and 60 ° C. for 1 hour, and then resolved for 1, 3, 5, and 26 hours, respectively.

As a result, as shown in FIG. 3, there was no difference in enzyme activity at 25 and 37 ° C. However, when the enzyme was treated at 60 ° C., the activity was confirmed late. In particular, the arazyme immobilized on the hydrogel appeared late at 60 ° C., but the arazyme of the stock solution did not show activity (FIG. 3). As a result, it was confirmed that the arazyme was safely immobilized by the hydrogel as well as the stability to heat by the immobilization.

In addition, arazyme immobilized on a hydrogel is placed on a skim milk plate and left at 60 ° C. for 1/2, 1, 2 and 4 hours, and then left for 6, 20, 30 and 40 hours to change the arazyme activity. I looked at it.

As a result, as shown in FIG. 4, it was confirmed that the longer the recovery time, the longer the active recovery time was (FIG. 4). In addition, it was confirmed that the protein or keratin through recovery was decomposed within the range where the enzyme activity was not lost by immobilization.

< Example  2> containing natural functional substances Hydrogel  Produce

Based on the measurement results of the physical properties of the <Experimental Example 1> hydrogel containing a natural functional material having a composition of the following [Table 9] was prepared by applying the type C in the basic prescription. Type A applied cosmetic ingredients and moisturizers to the basic formulation, type B and C applied high HLB nonionic surfactants, and type D prepared by liposomes of arazyme and cosmetic ingredients.

Prize ingredient A type Type B C type D type A Glycerin 20.0 20.0 20.0 10.0 Phenonip 0.3 0.3 0.3 0.3 Sodium polyacrylate
[sodium polyacrylate (MW: 4,500 ~ 5,000)] 3.0 3.0 3.0 3.0 Aluminum chloride
AlCl ₃ (10% Soln.aq.) 3.0 3.0 3.0 3.0 Sodium-carboxymethyl cellulose (Na-CMC) 1.0 1.0 1.0 1.0 Portulaca extract 3.0 3.0 3.0 3.0 Collagen liquid 1.0 1.0 1.0 1.0 KM205C - 1.0 - - ZKS-1670 - - 1.0 - Bio-PGA 3.0 3.0 3.0 3.0 1,3-butylene glycol (1.3-BG) - - - 10.0 B Polyacrylic acid
[polyacrylic acid (MW: 250,000 ~ 300,000)] 6.0 6.0 6.0 6.0 Arazyme 0.2 0.2 0.2 - Chlorella Extract (chlorella ext.aq) 0.5 0.5 0.5 0.5 SG - 0.1 - - Liposome base - - - 5.0 EGF Soln. 1.0 1.0 1.0 1.0 D.I.Water 58.0 56.9 57 53.2 Total amount 100.0 100.0 100.0 100.0

< Experimental Example  5> containing natural functional substances Hydrogel  Adhesion evaluation

<5-1> Physical property evaluation

The type A, B and C hydrogels prepared in Example 2 were cut to a size of 30 × 200 (mm) to attach the surface on which the nonwoven fabric was attached to a 30 ° inclined plate (transparent plastic plate). The release film was then removed. Rolling a high-strength chrome plated ball (stopping for more than 5 seconds) was evaluated by recording the largest ball size and number, and the experiment was conducted five times in a row.

As a result, as shown in [Table 10], the hydrogel to which KM205C (type B) or ZKS-1670 (type C) was added was not added. Adhesive strength was better than that of A, but no significant difference was found (Table 10).

Physical adhesive evaluation result of hydrogel Ball diameter (in) Distance (cm) One 2 3 4 5 6 7 8 9 10 Average Example 2
-A type 15/32 4.8 5.5 5.7 5.1 4.8 5.3 5.9 5.7 5.1 5.1 5.30 Example 2-B 15/32 5.1 5.4 5.3 5.1 4.8 5.1 4.9 4.8 5.0 4.8 5.03 Example 2-C 15/32 4.8 5.1 5.4 4.7 4.6 5.2 4.9 5.0 5.1 5.3 5.01

<5-2> sensory evaluation

According to the sensory evaluation criteria as shown in [Table 11] to 10 test subjects in order to check the adhesion of the type B and C of the <Example 2> containing a non-ionic surfactant to improve the adhesion, skin adhesion The test was conducted.

As a result, as shown in [Table 12], the B-type hydrogel showed an opinion that the adhesion is stronger. The adhesion of the type B hydrogel is stronger but not inconvenient, and when adhered to the skin, it does not flow out of the skin, and no particular problem occurs during adhesion to the skin. It also had the property of not sticking to the skin and leaving no residue when peeled off (Table 12).

Sensory Evaluation Criteria for Evaluating Adhesion of Hydrogels Score Evaluation standard One No stickiness on skin 2 Weak adhesion on skin 3 Moderate adhesion on skin 4 Strong adhesion on the skin 5 Very strong adhesion on the skin

Experimental Evaluation Results of Adhesion of Hydrogels experimenter One 2 3 4 5 6 7 8 9 10 Average Evaluation
end Example 2-A 3 3 4 4 3 2 3 3 3 3 3.10 Example 2-B 3 4 4 4 4 3 4 4 3 3 3.60 Example 2-C 3 3 3 3 3 3 4 2 3 3 3.00

< Experimental Example  6> containing natural functional substances Hydrogel  Stability over time

In order to confirm the stability of the D-type hydrogel of <Example 2> containing liposome-ized arazyme and cosmetic ingredients over time, the color, odor, moisture retention and weight for 3 months at 25 ℃, 43 ℃ and UV Changes, appearance modifications, pH and microbial tests were performed.

As a result, as shown in Table 13, no change was observed in the appearance of the hydrogel under various harsh conditions, and the pH value was found to be almost unchanged. The microbial test also showed that the microbial acceptance criteria (≦ 100 cfu / ml) were met (Table 13).

Stability Changes with Temperature and Time Duration (days) 0 One 3 5 7 14 21 30 40 70 90 25 ℃ Appearance /
Aroma clear clear clear clear clear clear clear clear clear clear clear pH 5.25 5.24 5.25 5.25 5.23 5.27 5.26 5.26 5.26 5.25 5.23 microbe 0 0 0 0 0 10 10 0 0 10 0 40 ℃ Appearance /
Aroma clear clear clear clear clear clear clear clear clear clear clear pH 5.25 5.23 5.27 5.27 5.29 5.24 5.24 5.22 5.22 5.23 5.22 microbe 0 0 0 0 0 0 0 0 0 0 0 UV Appearance /
Aroma clear clear clear clear clear clear clear clear clear clear clear pH 5.25 5.27 5.27 5.26 5.26 5.26 5.25 5.26 5.23 5.26 5.26 microbe 0 0 0 0 0 0 0 0 0 0 0

Experimental Example 7 Evaluation of Water Retention Capacity of Moisturizer of Hydrogel Containing Natural Functional Substance

The moisturizing effect on the four moisturizers was evaluated by its own water retention capacity. Glycerin, 1,3-butylene glycol (1,3-BG), sodium hyaluronate (1% solution, Na-Hyal)}, sorbitol In order to measure the water retention of sorbitol, each sample was prepared in 50% aqueous solution. About 10 g of the sample was taken and the moisture content was measured with a halogen moisture analyzer until the content was at 250 ° C. for 250 minutes.

As a result, as shown in [Table 14], the water retention capacity was the best sodium hyaluronate, followed by sorbitol, glycerin, 1,3-butylene glycol (Table 14).

Moisture retention test results Moisturizer glycerin 1,3-BG Na-Hyal Sorbitol Moisturizing effect 4.57% 2.11% 21.15% 10.58%

< Experimental Example  8> containing natural functional substances Hydrogel  Moisturizing effect measurement

In order to measure the moisturizing effect of the moisturizing component, the hydrogel was prepared according to the formulation of the following [Table 15] according to the test result of the moisture retention ability of the <humidifier 7>. Hydrogel's moisturizing effect was measured in 10 healthy women (or men). Hydrogel was attached to the inside of the upper arm of the tester with a size of 10 × 10 (mm), and then attached using a tape of a fin chamber and a hill top chamber having a diameter of 12 mm, and the sample was 3 hours later. 20 minutes after the removal, the moisture content was measured using a skin moisture meter, and the sample was remeasured 2 hours after removing the sample. The measurement of the moisturizing effect was carried out inside a constant temperature and humidity chamber where humidity can be known.

As a result, as shown in [Table 16], the overall hydrogel showed an excellent moisturizing effect, especially in the case of the hydrogel containing sodium hyaluronate, hydrogel without addition of sodium hyaluronate and arazyme (Blnak) Compared with, the moisturizing effect was excellent (Table 16).

Prescription for Moisturizing Effect of Hydrogel Prize ingredient Blank T-1 T-2 A Glycerin 20.0 20.0 20.0 Phenonip 0.3 0.3 0.3 Methyl Polyacrylate
{sodium polyacrylate (MW: 4,500-5,000)} 3.0 3.0 3.0 Aluminum chloride {AlCl ₃ (10% Soln.aq.)} 3.0 3.0 3.0 Sodium-carboxymethyl cellulose (Na-CMC) 1.0 1.0 1.0 Portulaca extract 3.0 3.0 3.0 Collagen liquid 1.0 1.0 1.0 Bio-PGA 3.0 3.0 3.0 B Polyacrylic acid
{polyacrylic acid (MW: 250,000 ~ 300,000) 6.0 6.0 6.0 Arazyme - - 0.2 Sodium hyaluronate
{sodium hyaluronate (1% soln.)} - 5 5 D.I.Water 59.7 54.7 54.5 Total amount 100.0 100.0 100.0

Moisturizing Effect of Hydrogel with Moisturizer Prescription Before attach 3 hours after attachment 2 hours after removal Blank 50.1 120.2 100.1 T-1 49.8 207.5 157.5 T-2 47.6 221.1 165.1

As described above, the skin care mask using the hydrogel containing arazyme of the present invention can be usefully used as a formulation for skin care that continuously delivers the exfoliation effect of arazyme by immobilization, and includes an effective cosmetic ingredient. It can be usefully used for the development and application of the skin care mask of the hydrogel formulation.

1 is a diagram showing the sampling for comparison of the distribution of each site in the hydrogel of arazyme.

2 is a diagram confirming the distribution of arazyme in the hydrogel.

Figure 3 is a figure confirming the stability of arazyme by the immobilization of hydrogels.

Figure 4 is a figure confirming the stability through the recovery of the activity of arazyme by the immobilization of hydrogel.

<110> Insect Biotech <120> Skin care mask using hydrogel containing arazyme. <130> 9p-06-26 <160> 2 <170> KOPATIN 1.5 <210> 1 <211> 487 <212> PRT <213> Aranicola proteolyticus <220> <221> BINDING <222> (192) <223> Zn binding site <220> <221> BINDING <222> (196) <223> Zn binding site <220> <221> BINDING <222> (323) <223> Zn binding site <400> 1 Met Gln Ser Thr Lys Lys Ala Ile Glu Ile Thr Glu Ser Ser Leu Ala   1 5 10 15 Ala Ala Ser Ser Ala Tyr Asn Ala Val Asp Asp Leu Leu His Tyr His              20 25 30 Glu Arg Gly Asn Gly Ile Gln Val Asn Gly Lys Asp Ser Phe Ser Thr          35 40 45 Glu Gln Ala Gly Leu Phe Ile Thr Arg Glu Asn Gln Thr Trp Asn Gly      50 55 60 Tyr Lys Val Phe Gly Gln Pro Val Lys Leu Thr Phe Ser Phe Pro Asp  65 70 75 80 Tyr Lys Phe Ser Ser Thr Asn Val Ala Gly Asp Thr Gly Leu Ser Lys                  85 90 95 Phe Ser Ala Glu Gln Gln Gln Gln Ala Lys Leu Ser Leu Gln Ser Trp             100 105 110 Ser Asp Val Ala Asn Ile Thr Phe Thr Glu Val Gly Ala Gly Gln Lys         115 120 125 Ala Asn Ile Thr Phe Gly Asn Tyr Ser Gln Asp Arg Pro Gly His Tyr     130 135 140 Asp Tyr Asp Thr Gln Ala Tyr Ala Phe Leu Pro Asn Thr Ile Tyr Gln 145 150 155 160 Gly Gln Asn Leu Gly Gly Gln Thr Trp Tyr Asn Val Asn Gln Ser Asn                 165 170 175 Val Lys His Pro Ala Ser Glu Asp Tyr Gly Arg Gln Thr Phe Thr His             180 185 190 Glu Ile Gly His Ala Leu Gly Leu Ser His Pro Gly Asp Tyr Asn Ala         195 200 205 Gly Glu Gly Asn Pro Thr Tyr Arg Asp Ala Ser Tyr Ala Glu Asp Thr     210 215 220 Arg Glu Phe Ser Leu Met Ser Tyr Trp Ser Glu Thr Asn Thr Gly Gly 225 230 235 240 Asp Asn Gly Gly His Tyr Ala Ala Ala Pro Leu Leu Asp Asp Ile Ser                 245 250 255 Ala Ile Gln His Leu Tyr Gly Ala Asn Gln Thr Thr Arg Thr Gly Asp             260 265 270 Thr Val Tyr Gly Phe Asn Ser Asn Thr Gly Arg Asp Phe Leu Ser Thr         275 280 285 Thr Ser Asn Pro Gln Lys Val Ile Phe Ala Ala Trp Asp Ala Gly Gly     290 295 300 Asn Asp Thr Phe Asp Phe Ser Gly Tyr Thr Ala Asn Gln Arg Ile Asn 305 310 315 320 Leu Asn Glu Lys Ser Phe Ser Asp Val Gly Gly Leu Lys Gly Asn Val                 325 330 335 Ser Ile Ala Ala Gly Val Thr Ile Glu Asn Ala Ile Gly Gly Ser Gly             340 345 350 Asn Asp Val Ile Val Gly Asn Ala Ala Asn Asn Val Leu Lys Gly Gly         355 360 365 Ala Gly Asn Asp Val Leu Phe Gly Gly Gly Gly Ala Asp Glu Leu Trp     370 375 380 Gly Gly Ala Gly Lys Asp Thr Phe Val Phe Ser Ala Val Ser Asp Ser 385 390 395 400 Ala Pro Gly Ala Ser Asp Trp Ile Lys Asp Phe Gln Lys Gly Ile Asp                 405 410 415 Lys Ile Asp Leu Ser Phe Phe Asn Gln Gly Ala Gln Gly Gly Asp Gln             420 425 430 Ile His Phe Val Asp His Phe Ser Gly Ala Ala Gly Glu Ala Leu Leu         435 440 445 Ser Tyr Asn Ala Ser Asn Asn Val Ser Asp Leu Ala Leu Asn Ile Gly     450 455 460 Gly His Gln Ala Pro Asp Ile Leu Val Lys Ile Val Gly Gln Val Asp 465 470 475 480 Val Ala Thr Asp Phe Ile Val                 485 <210> 2 <211> 2481 <212> DNA <213> Aranicola proteolyticus <400> 2 cgagcgcgaa agacccggaa ggcacgaggt gattagtcaa aaaaagaaaa tgttattcct 60 gcgggaacta aaaagtaccg gcggctaata ataaagagtt attaatctat aacgctttag 120 ccaaatttaa cttttagccg tctaaatccc agcacgattc gcttggctct gcaggccgca 180 tttttgttgg agtttgttac caactcatgg catttaagtt tcattaatat tgtaaataat 240 gcaaaaaacc agcataaatc cccttcgtaa cgataataaa tggctgatta ttttatgtgc 300 agttttacac cgctgcctat aattggaatc gattaccatt tatggtggta atcttatttg 360 ctgatatata tgcattaatt ctctctaaca cactgccggt ancggcgcat aaactccttc 420 ccgtaagcgt gcggttcgtt ctccgtggct tcctggcagg ttatgtctat ctgtctgatt 480 gaaaccaatc agctaatgag tggaatcgaa ccaatgcaat ctactaaaaa ggcaattgaa 540 attactgaat ccagccttgc ggctgcgagc tccgcttaca atgcagtaga tgatttgctg 600 cattatcatg agcgaggcaa cgggattcag gttaatggca aggactcatt ttctaccgaa 660 caagccgggc tgtttattac ccgcgagaac caaacctgga acggttataa agtttttggc 720 caaccggtta aattaacgtt ctctttcccg gattataaat tctcttccac caacgtcgcc 780 ggcgataccg gactgagcaa attcagcgcg gaacagcagc agcaggctaa gctgtcgctg 840 cagtcctggt ctgacgtggc caatatcacc tttaccgaag ttggtgccgg ccagaaggcc 900 aatatcacct tcggtaacta cagccaggat cgtcccggcc attatgacta cgatacccag 960 gcttacgcct tcctgccgaa caccatttat cagggccaaa acctgggcgg gcagacttgg 1020 tacaacgtca accagtccaa cgtgaaacat ccggccagcg aagactacgg ccgccagacc 1080 tttacccacg agattggcca tgcgttgggc ttgagccatc cgggcgatta caacgccggc 1140 gaaggcaacc cgacttacag agatgccagc tacgccgaag atactcgtga gttcagcctg 1200 atgagttact ggagcgaaac caacaccggt ggcgacaacg gcgggcacta cgctgcggcg 1260 ccactgctgg atgacatttc cgctattcag catctgtatg gtgccaacca gaccacccgt 1320 accggcgata ccgtgtatgg cttcaactca aataccggac gtgacttcct cagtaccacc 1380 agcaatccgc aaaaagtgat ctttgcggcc tgggatgcgg gtggtaatga caccttcgat 1440 ttctccggtt acaccgctaa ccagcgtatt aatctgaacg agaaatcttt ctccgacgtg 1500 ggtgggctga aaggcaacgt gtccattgcc gcaggtgtga ccatcgagaa cgcgattggc 1560 ggttcaggca atgacgtgat cgtcggcaat gcggccaaca acgtgctgaa aggtggcgcg 1620 ggcaacgacg tgctgttcgg cggtggtggg gctgatgagc tgtggggcgg tgcgggcaaa 1680 gacacctttg tcttctctgc ggtcagcgat tctgcgccgg gtgcctccga ctggatcaag 1740 gatttccaga aaggcatcga taaaatcgac ctgtcattct tcaatcaggg cgcgcagggt 1800 ggcgatcaga tccacttcgt cgatcatttc agtggcgcag cgggcgaagc cttgctgtct 1860 tacaatgcgt cgaataacgt cagcgatctg gccctgaata tcggcggcca tcaggccccg 1920 gacatcctgg tgaagatcgt cggccaggtt gatgtcgcca ctgactttat cgtttaacag 1980 tgcaggtgct aacgcccggc gccggttggc cgggcgttat acaggagacg atatgaaggg 2040 cagcttagcg cacgccgcct tagtggcagg cggcatgatg gttacggggg cagttatggc 2100 cagcagtttg gttcttccca gcgcgcaatc attggcgggg caatggctgg tcgccaatgc 2160 cgaacaacaa tgtcagattg agtttttggc cggtgaacag agtgaaatca acggctactc 2220 attggttgat cggcagcact gtttggaaaa ggtgttaacc gccgaggtgg tcggttggcg 2280 ccctgcaccg gacggcatcg ctttgctgcg ggcggatggc agtacgctgg cgttcttctc 2340 gcgcgatggc gatatttacc gcaaccagct tggcgcggat gacggactga cgctgaaagc 2400 gctggtataa caacagcggg ttcggcagtc gaacccgccc tgagcagcct tacagataca 2460 gcgaacgtac gatcaggaaa t 2481  

Claims (14)

 Skin care hydrogel mask containing arazyme. The method of claim 1, wherein the arazyme, 1) a protein comprising the amino acid sequence set forth in SEQ ID NO: 1; 2) a protein encoded by a DNA (DNA) comprising a coding region of the nucleotide sequence set forth in SEQ ID NO: 2; 3) a protein consisting of an amino acid sequence in which one or more amino acids in the amino acid sequence set forth in SEQ ID NO: 1 is substituted, deleted, inserted and / or added, and which is functionally identical to the protein comprising the amino acid sequence set forth in SEQ ID NO: 1; And 4) a protein encoded by a DNA that hybridizes under stringent conditions to a DNA comprising the nucleotide sequence of SEQ ID NO: 2, and functionally identical to a protein comprising the amino acid sequence of SEQ ID NO: 1 Skincare hydrogel mask, characterized in that any one selected from the group consisting of. According to claim 1, wherein the hydrogel is a skin care hydrogel mask, characterized in that using a polymer and a crosslinking agent. The skin care hydrogel mask according to claim 3, wherein the polymer contains 2.0 to 4.0 parts by weight of sodium polyacrylate. The skin care hydrogel mask according to claim 3, wherein the crosslinking agent contains 2.0 to 4.0 parts by weight of aluminum chloride, which is a water-soluble polyvalent metal salt. The method of claim 1, wherein the hydrogel is purified water (water), glycerin (glycerin), poverty (chondrus crispus), Fiji-60 hydrogenated castor oil (PEG-60 hydorgenated castor oil), dipotassium glycitrate dipotassium glycyrrhizate, methylparaben, propylparaben, propylparaben, disodium EDTA, potassium hydroxide, and sodium hyaluronate Skin care hydrogel mask, characterized in that it further comprises any one or more. The method of claim 1, wherein the hydrogel is a protease, plant extract, water-soluble collagen (hydrolyzed collagen), ubiquinone (ubiquinone), tocopheryl acetate (arcotin), arbutin, colloidal platinum (colloidal platinum) Skincare hydrogel mask further comprises any one or more from the group consisting of enzymes, ceratiopeptidase, lipase, cellulase and xylanase. The method according to claim 7, wherein the protease is selected from the group consisting of papain, vegetable protease such as bromelain, animal protease such as trypsin and microbial derived protease such as Bacillus, Aspergillus Protease, characterized in that any one or more. The method of claim 7, wherein the plant extract is Portulaca oleracea extract, arnica montana flower extract, gentiana lutea root extract, acheillea millefolium extract, mugwort extract artemisia vulgaris extract) and chamomilla recutita flower extract. 1) preparing a hydrogel by mixing and stirring arazyme, synthetic polymer and crosslinking agent; And 2) skincare hydrogel mask manufacturing method comprising the step of preparing the hydrogel in the form of a mask. The method of claim 10, wherein the arazyme, 1) a protein comprising the amino acid sequence set forth in SEQ ID NO: 1; 2) a protein encoded by a DNA (DNA) comprising a coding region of the nucleotide sequence set forth in SEQ ID NO: 2; 3) a protein consisting of an amino acid sequence in which one or more amino acids in the amino acid sequence set forth in SEQ ID NO: 1 is substituted, deleted, inserted and / or added, and which is functionally identical to the protein comprising the amino acid sequence set forth in SEQ ID NO: 1; And 4) a protein encoded by a DNA that hybridizes under stringent conditions to a DNA comprising the nucleotide sequence of SEQ ID NO: 2, and functionally identical to a protein comprising the amino acid sequence of SEQ ID NO: 1 Skin care hydrogel mask manufacturing method characterized in that any one selected from the group consisting of. The method of claim 10, wherein the hydrogel uses a polymer and a crosslinking agent. 13. The method of claim 12, wherein the polymer contains 2.0 to 4.0 parts by weight of sodium polyacrylate. The method of claim 12, wherein the crosslinking agent comprises 2.0 to 4.0 parts by weight of aluminum chloride, which is a water-soluble polyvalent metal salt.

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