KR20100129571A - Functional composition for improvement of sex and food having the same - Google Patents

Abstract

PURPOSE: A composition for improving sexual function and a food containing the same are provided to improve sexual functional by vasodilation and hormone balance. CONSTITUTION: A composition for improving sexual function contains 30-70 weight% of Lepidium meyenii, 40-60 weight% of red ginseng, and 20-40 weight% of Tribulus alatus extract as an active ingredient. The composition is fermented by adding Lactobacillus(Leuconostoc sp., Lactobacillus sp., Lactococcus sp., Bifidobacterium sp., and Streptococcus sp.) only or together with a starter. A food for improving sexual function contains the extract as an active ingredient. The food includes gum, tea, vitamin complex, health supplement, and honey.

Description

Sexual composition for improving sperm count and preventing environmental hormones and food containing the same {Functional composition for improvement of sex and food having the same}

The present invention relates to a functional composition comprising extract complex fermentation of maca, red ginseng, namgasae for improving sexual function and increasing sperm count and preventing male reproductive function due to environmental hormones, specifically by vasodilation and hormonal balance The present invention relates to a composition for improving sexual function having an excellent effect on improving sexual function and increasing sperm count.

Erectile erection of sexually excited penis occurs due to the complex interactions of physiological processes including the central nervous system, peripheral nervous system and smooth muscle. In particular, the release of nitric oxide from non-adrenergic, non-cholinergic nerves and endothelium activates guanyl cyclase in the corpus cavernosum and increases intracellular cGMP levels. Increasing intracellular cGMP reduces intracellular calcium levels, which lead to columnar smooth muscle relaxation, which in turn leads to cavernous volume expansion and compression of sub-tunical venules leading to penile erection.

In addition, ACE (angiotesin converting enzyme), which is involved in the synthesis of angiotensin II, a potent vasoconstrictor in the human body, decreases the activity of ACE and increases nitric oxide in the blood to promote blood vessel relaxation, thereby promoting blood flow in the penis. This increase can also lead to penile erections.

Modern living environment confuses biological rhythms due to overwork, drinking, stress, and environmental pollution. As the frequency of adult diseases such as hypertension, diabetes, and obesity increases, energy declines from the 20s and 30s. The symptoms of aging, poor sexual function, and so on, are often seen, and at the age of 40, they are very serious. In particular, the number of men suffering from various male sexual dysfunction such as erectile dysfunction, infertility, infertility, sexual dysfunction is increasing.

Due to environmental pollution, endocrine disruptors (Endocrine Disrupters) enter the human body to interfere with or disrupt the action of hormones, the effects of environmental hormones are severe. These environmental hormones can cause reproductive disorders or sexual transformation even in very small amounts, especially in men, leading to erectile dysfunction, decreased sperm count and semen, decreased sperm motility, increased malformation of sperm, genital malformations, and testicular tumors. . As environmental hormones, 68 kinds of chemicals such as dioxins, persistent organic chlorine, pesticides, and phenols are registered.In particular, cadmium is easily absorbed by the human body as a heavy metal that causes osteopenia, renal dysfunction, and neurological disorders as well as testicular damage. It is recognized as one of the most important environmental hormones causing infertility.

Recently, sildenafil (viagra), a phosphodiesterase 5 (PDE5) inhibitor, has been approved by the FDA as an effective oral treatment for erectile dysfunction. By inhibiting PDE5, it inhibits cGMP degradation, which plays an important role in penile erection, increases intracellular cGMP content and induces penile erection under sexual excitement. This drug improved sexual function in approximately 70% of men suffering from erectile dysfunction of psychological or biological causes. However, the use of sildenafil is associated with several undesirable side effects, including headaches, flushing and color confusion due to non-selective effects on various tissues, and disorders of the body related to blood circulation and endocrine function, which cause erectile dysfunction. As a result of development and use focusing on temporary erectile induction without improving it, resistance or various side effects may occur.

In addition, the drug treatment for erectile dysfunction in men has been used, such as amino acid therapy, vitamin therapy, sex hormone therapy, but the situation is still not satisfactory, and as a treatment for sperm reduction, systemic therapy, drugs There are medical therapies, but the success rate is very low.

In Korea, the treatment of male sexual functioning relies on Korean medicine for sociological and ethical reasons, but most of them do not have the correct mechanism of action and basic systematic research on each prescription. Sexual Improvement Health foods are also mixed with ingredients that are known to be good for improving sexual function, ignoring scientific research such as the proper amount of active ingredients, synergistic effects, and the duration of taking them.

Therefore, there is an urgent need for the development of natural products of new oral ingredients that have sufficient effects without side effects.

The main compositions used in the present invention are as follows.

Maca ( Lepidium meyenii ) is a perennial plant that grows in the highlands of 4000 m above the Andes and is succulent. Fruits are harvested in May-June with the size of 4-5 mm. The active ingredients of maca include beta-sitosterol, saponin, stigmasterol, tannin, alkaloids, and beta-tectisone, and contain a large amount of zinc, which is known to promote the development of sperm and eggs, thereby normalizing endocrine hormone activity in the body. It is said to be effective in improving sexual function through nourishing or tonic nourishment. In addition, it is known that it is rich in essential amino acids arginine and lysine, thus increasing sperm count and egg fertilization rate and contributing to overcoming infertility.

Trigasus alatus is an annual plant that grows on the sandy beaches of Jeju Island, Geoje Island and Myeongcheon County, North Hamgyong Province. Stems grow about 1 meter long, branched from the bottom, creeping sideways, leaves facing each other, 5-7 pairs of oval-shaped leaves. Yellow flowers bloom in summer, polygonal fruits with sharp thorns ripen in autumn. The Chinese characters are called 'baekji-ri' or 'jaji-ri', and the fruits are called 'ji-gi-ri' or 'baek-seok-ri', 'seok-ri' and 'sil-ja'. The fruit of Namgasae contains saponins, essential oils, alkaloids, gums, potassium salts, etc. It is known to soften blood vessels, dissolve cholesterol, improve blood circulation, lower blood pressure, eliminate inflammation, and act as a diuretic.

Red ginseng is steamed and dried from perennial herb, Panax ginseng , which belongs to the Ogalpiaceae. have. The main component of red ginseng is triterpenoid-based ginseng saponin, which has been shown to have a nourishing tonic effect of enhancing the nonspecific resistance of the body to stress and maintaining the homeostasis of the body. In addition, it has been found that there are improvements in hypertension, enhanced insulin action, hypoglycemic effect, and anticancer effect.

Fermentation is a process in which microorganisms use their own enzymes to decompose organic matter, which is similar to the decay reaction, but it is called fermentation that produces substances useful for our lives. The microorganism used in the fermentation of the present invention is Lactobacillus sp. Through this fermentation process, the active ingredient of the plant extract is absorbed into the body, which shows the superior effect than the original effect. In addition, cancer prevention, blood cholesterol suppression, hangover recovery and other effects are known.

The inventors of the present invention increase the resistance and strength to stress, improve blood circulation and improve hormonal balance for the fundamental improvement of the obstacles to the sexual life of modern men through the fermentation process by selecting edible materials The composition was developed to maximize the effect of improving sexual function. This composition was confirmed by animal experiments and its effects were demonstrated through clinical trials to complete the present invention.

In addition, as a result of the finished composition, scientific experiments demonstrate the prevention effect of testicular tissue damage and sperm reduction caused by environmental hormones.

Therefore, an object of the present invention is to minimize the problems such as toxicity and side effects, lack of efficacy, and rejection of erectile dysfunction treatment agents, and to improve sexual function and composition containing sperm increase and environmental hormone prevention effect to maximize convenience and safety It is to provide food for improving sexual function.

In order to achieve the above object, the present invention is to maximize the active ingredient by mixing and fermenting maca extract, red ginseng extract, namgasae extract, maca extract of the extract provides a sexual function improving composition, characterized in that obtained by hot water extraction do.

Here, it is characterized in that it comprises 30 to 70% by weight of maca extract, 40 to 60% by weight of red ginseng extract, 20 to 40% by weight of namgasae extract based on the solid content.

It characterized in that it comprises the fermented one or more selected mixed extract of the extract crowd.

It is characterized in that the fermented by the addition of lactic acid bacteria (Lukeconstock, Lactobacillus, Lactococcus, Pediococcus, Bifidobacterium, Straptococcus) alone or as a mixed starter.

The lactic acid bacteria are Leuconostoc citreum , Leuconostoc gelidium , Leuconostoc mesenteroides sub. Mesente roides , and Lactococcus lactis , Lactococcus lactis subsp.lactis , Lactococcus plantarum and Lactobacillus brevis in Lactococcus plantarum , Lactobacillus plantarum , Lactobacillus plantarum , and Lactobacillus saculobacterus bacillus bacillus bacillus ( Lactobacillus bulgaricus ), Lactobacillus casei , Lactobacillus acidophilus and Streptocuccus thermophilus of Streptococcus and Bifidobacterium genus of Bifidobacterium Bifidobacterium bifidum , Bifidobacterium lact is ), Bifidobacterium longum and finally Lactic acid bacteria such as Pediococcus pentosaceus , Pediococcus massilacti in Pediococcus It is characterized by.

In addition, according to another field of the present invention, the above object is the maca extract, red ginseng extract, namgasae extract containing as an active ingredient, the maca extract of the extract is achieved by food for improving sexual function, characterized in that obtained by hot water extract do.

Gum, tea, vitamin complex, dietary supplements, honey products made from the composition is characterized in that it comprises.

In addition, it is characterized by consisting of powder, granules, tablets, capsules, pills or liquids.

Therefore, the present invention provides an effect of improving sexual function, increasing sperm count, and preventing male reproductive function caused by environmental hormones. It inhibits ACE (Angiotensin converting enzyme) and PDE (Phosphodiesterase) and increases serum cGMP content, causing erection through increased blood flow and relaxation of penile smooth muscle, and sexual function can be improved by increasing sex hormone. In addition, it is possible to prevent the reproductive function is reduced by cadmium chloride.

Hereinafter, the present invention will be described in detail.

The present invention provides a composition having a fermentation effect of one or more herbal extracts selected from the group consisting of maca, red ginseng and namgasae, having an effect of increasing sperm count and preventing male reproductive function due to environmental hormones. Preferably it provides a composition having improved sexual function and increased sperm count and male reproductive function lowering effect by environmental hormones containing a plant extract complex fermentation consisting of maca, red ginseng, namgasae.

Specifically, the composition is composed of 30 to 70% by weight of maca extract, 40 to 60% by weight of red ginseng extract, 20 to 40% by weight of Namgasae extract, characterized in that the fermentation process by lactic acid bacteria.

Maca extract of the present invention can be obtained as follows.

After washing the maca source, it is dried sufficiently in the shade and shredded or pulverized, and then water of about 1 to 30 times the weight, a lower alcohol such as ethanol or a mixed solvent thereof, preferably 20 to 100 ° C. with water, preferably Is 50 to 95 ℃ extraction temperature for 1 hour to 2 days, preferably 2 to 5 hours, 1 to 10 times, preferably 2 to 5 times repeatedly hot water extraction, ultrasonic extraction, reflux cooling extraction, etc. Extraction method of the extract, more preferably hot water extraction method to obtain the extract, and then filtered and concentrated under reduced pressure or vacuum to obtain an extract concentrate, additionally in the form of powder through freeze drying and hot air drying Can be obtained.

Red ginseng extract, namgasae extract used in the present invention was purchased from a raw material supply company. In order to prepare a plant extract fermentation compound, water was added to the composite material consisting of 30-70% by weight of maca extract, 40-60% by weight of red ginseng extract, and 20-40% by weight of Namgasae extract so that the humidity of the raw material was 50-70%. After sterilization for 20 to 40 minutes to prepare the sample, and after the addition of water in the ratio of 1:10 to 1:30 (w / v) during liquid fermentation, it is prepared by sterilization for 20 to 40 minutes. The Lactobacillus genus to be used for fermentation is added to the mycelium 3-10% (w / v) during solid fermentation, 2-10% (w / v) during liquid fermentation, and 4-9 at 10-30 ° C. Perform daily fermentation. The fermented product is sterilized for 10 to 30 minutes and then dried to obtain a powder form.

The present invention provides a method for producing a complex fermentation of maca, red ginseng, namgasae extract as described above.

In addition, the present invention provides an improved sexual function composition for improving sexual function, increasing sperm count and preventing male reproductive function lowering by environmental hormones.

In addition, the raw materials have been used as food for a long time as the composition of the present invention extracted from them also has no problems such as toxicity and side effects.

The ACE inhibitory effect, PDE inhibitory effect, cytotoxicity against testis cells and testosterone concentration change of the composition obtained by the above method were measured. In addition, animal experiments were conducted to investigate the effects of improving male sexual function and preventing male reproductive function caused by environmental hormones.

The sexual function improvement composition is included in an amount of 0.1 to 100% by weight based on the total weight of the functional food composition effective for improving sexual function and increasing sperm count and preventing male reproductive function caused by environmental hormones.

It may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of the functional food composition comprising the composition of the present invention.

Functional food compositions comprising the composition according to the invention can be used in the form of oral dosage forms, such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, etc., respectively, according to conventional methods. Carriers, excipients and diluents that may be included in the compositions comprising the compositions of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate , Calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, honey, methylhydroxybenzoate, propylhydroxybenzoate, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations include at least one excipient such as starch, calcium carbonate, sucrose or lactose, gelatin, and the like. Mix is prepared. In addition to simple excipients, lubricants such as magnesium stearate are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. .

Preferred dosages of the compositions of the present invention vary depending on the subject's condition and weight, extent of disease, drug form, route of administration and duration, and may be appropriately selected by those skilled in the art. However, for the desired effect, the composition of the present invention is preferably administered at 0.5 to 3 g per day. Administration may be once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

Since the composition of the present invention does not have toxicity and side effects, it can be used with confidence even for long-term use for prophylactic purposes.

The composition of the present invention can be used in various ways, such as food and beverages for improving sexual function and increasing sperm count and preventing male reproductive function caused by environmental hormones. Foods to which the composition of the present invention may be added include various foods, for example, beverages, gums, teas, vitamin complexes, dietary supplements, honey products, and the like, pills, powders, granules, tablets, capsules or beverages. Available in form. At this time, the amount of the composition in the food or beverage may be added in 0.01 to 15% by weight of the total food weight, the health beverage composition may be added in a ratio of 0.02 to 5 g based on 100 ml.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and fillers (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and salts thereof. , Organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the composition of the present invention may contain a fruit flesh for the production of natural fruit juices and fruit juice drinks and vegetable drinks. These components can be used independently or in combination. The proportion of such additives is not so critical, but is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

However, the following Examples and Experimental Examples are only for illustrating the present invention, and the content of the present invention is not limited to the following Examples and Test Examples.

Example 1 Preparation of Extract

Maca, red ginseng extract, namgasae extract used in the present invention was purchased from a raw material supply company. To prepare the maca extract, 1 kg of maca was dried, and then 300 g of the powder obtained by crushing was put in a household jar, 1800 ml of water was added and extracted twice at 50 ° C. for 2 hours, and then, filter paper (Whatman No. 1, England) After filtration under reduced pressure with filtration, the extract was concentrated under reduced pressure at 40 ° C. with a vacuum rotary condenser and dried to obtain an extract.

Example 2. Fermentation

Maca extract, red ginseng extract, and Namgasae extract of Example 1 were added at a blending ratio of 50 g, 30 g, and 20 g, respectively, based on the solids content weight, and water was added to make the humidity of the raw material 50%, followed by 20 minutes at 121 ° C. Sterilize. After incubation at 30 ° C. for 28 hours in MRS broth (Difco, France), 6 g of pellets of Lactobacillus obtained by centrifugation were inoculated into sterilized plant extract complexes and fermented at 30 ° C. for 7 days. The fermented product was sterilized at 121 ° C. for 20 minutes and then dried to prepare a performance improving composition (hereinafter referred to as 'MP119' in each experiment).

Experimental Example 1. Measurement of inhibitory effect of ACE (angiotesin converting enzyme)

ACE inhibitory activity was measured by applying the method of Cushman et al. First, in order to prepare an enzyme source, 10 g of rabbit lung acetone powder is sufficiently mixed with 100 ml of 50 mM potassium phosphate buffer (pH 8.3), and the supernatant obtained by centrifugation at 40,000 x g for 40 minutes is used as an enzyme source. The ACE inhibitory activity was first measured by using a 0.25 ml [100 mM potassium phosphate buffer (pH 8.3), 300 mM NaCl, 5 mM hippuryl-his-leu], 0.15 ml (8 mU) enzyme, and 0.1 ml of a sexual improvement composition for 30 minutes. The reaction is carried out at 37 ° C. To stop the reaction by adding 0.25 ml of 1 N HCl, add 1.5 ml of ethyl acetate, and vortex to separate the ethyl acetate layer. The ethyl acetate layer was dried and then dissolved in distilled water, and the absorbance was measured at 229 nm to calculate the ACE inhibition rate (Table 1).

ACE inhibition rate is calculated as {1-[(A sample-A control group / B sample-B control group)] * 100}, and sample A is absorbance of the sexual function improving composition and the reaction group to which the enzyme source is added. Absorbance of the reaction group with added enzyme source but no enzyme source, sample B, absorbance of reaction group with no enzyme function added, B control group with reaction function without both sex function composition and enzyme source added Absorbance is shown.

As a result of the experiment, it can be seen that the higher the concentration of the sexual enhancement composition is increased ACE inhibition rate. IC ₅₀ (Inhibitory Concentration) value of the concentration of the sexual improvement composition that ACE is inhibited by 50% is 231.85 ppm and it was confirmed that it effectively inhibits ACE. Through this result, it is expected that the sexual improvement composition will help to maintain the erection, and also the blood pressure lowering effect due to the blood circulation improvement.

Experimental Example 2. Measurement of Inhibitory Activity of Phosphodiesterase5 (PDE5)

5-GMP was treated with 5-nucleotidase followed by BIOMOL GREEN ^TM Reagent to measure absorbance at 620 nm to obtain a standard curve, and reacted by adding PDE and sexual function composition using 3, 5-cGMP as a substrate. Treatment with 5-nucleotidase and treatment with BIOMOL GREEN ^™ Reagent measured the absorbance at 620 nm, and the PDE inhibition rate was calculated by comparing the amount of 5-GMP produced with the control group.

Experimental Example 3. Confirmation of cytotoxicity against mouse testicular cell TM3

In order to examine the cytotoxicity of the sexual improvement composition, MTT assay was performed by Sladowski's method using Leydig cells (TM3, Korea Cell Line Bank), which are testis cells of male mice. 90 μl of TM3 cells (2 × 10 ⁵ cells) were treated with 10 μl of a sexual improvement composition, followed by 5 hours of incubation at 37 ° C., followed by 10 μl of MTT solution (5 mg / ml), followed by 3 hours of incubation at 37 ° C. After removal of the supernatant, 200 μl of DMSO was added per well, the reaction was performed for 20 minutes, and the absorbance was measured at 595 nm. The survival rate (%) is shown in Fig. 1 by calculating from [(absorbance of control group-absorbance of experimental group) / absorbance of control group] x 100.

As a result of the experiment, as shown in Figure 1, the sexual improvement composition was found to be cytotoxic up to 1000 ppm.

Experimental Example 4 Testosterone Concentration Measurement in TM3 Cells

Testosterone concentrations in TM3 cells are measured using the Enzyme Immunoassay Kit (Assay Designs, USA). 90 μl of cells (2 × 10 ⁵ cells) were treated with 10 μl of the sexual improvement composition and then incubated at 37 ° C. for 48 hours. After incubation, transfer 100 µl of the supernatant to goat anti-mouse IgG microtiter plate, incubate for 1 hour with testosterone EIA antibody, and incubate for 1 hour with conjugate. After washing, the solution was added to pNpp substrate solution to stop the reaction after 1 hour of reaction, and the absorbance was measured at 405 nm.

As shown in FIG. 2, testosterone concentrations were not changed up to 5, 10, and 50 ppm of treatment groups by concentration of sexual improvement composition, but 12.09, 12.43, 12.93, and 13.68 pg / ml at 100, 200, 400, and 1000 ppm, respectively, compared to the control group. Up to 20.11% increase can be seen. Through this result, it can be confirmed that the sexual improvement composition promotes testosterone secretion in the testis.

Experimental Example 5. Confirmation of Sexual Function Enhancement and Sperm Count Increase in Rats

As the experimental animals, 10 male rats (ICR mice, 7 weeks old at the time of purchase) were used in each group, and were divided into a control group and a sexual improvement composition group.

In a constant environment of room temperature 22 ㅁ 1 ℃, humidity 55 ㅁ 5%, contrast cycle 12 hours, the animals were bred to avoid possible stress, and the control group administered distilled water and the experimental group administered 30 mg / mice / day to the sexual improvement composition, respectively. It is administered orally once a day for 7 days. After the last oral administration, the mice were sacrificed to separate testes, testes, and seminal vesicles, and their respective weights were measured. The isolated testes were homogenized in 2 ml of physiological saline, and allowed to stand at 37 ° C. for 20 minutes. Dilute to 5 and measure sperm count on microscope.

The weights of the testis, spermatozoa and seminal vesicles are shown in Table 3 and the number of sperm is shown in FIG.

Experimental Example 6. Effect of Environmental Hormone in Rats Administered with Cadmium Chloride

As the experimental animals, 10 male rats (ICR mice, 7 weeks old at the time of purchase) were used in each group, and were divided into a control group and a sexual improvement composition group.

In a constant environment of room temperature 22 ㅁ 1 ℃, humidity 55 ㅁ 5%, contrast cycle 12 hours, the animals were bred to avoid possible stress, the control group administered distilled water and the experimental group administered 30 mg / mice / day to the sexual improvement composition, respectively. It is administered orally once a day for 7 days. After the last oral administration, cadmium chloride was injected once intraperitoneally at 0.06 mg / mice, and after 24 hours, mice were sacrificed to separate testes, testes, and seminal vesicles, and weighed each. After homogenization in saline solution, the mixture is allowed to stand at 37 ° C. for 20 minutes, and the mixture is diluted 1/5 to measure sperm count on a microscope.

The weights of the testis, spermatozoa and seminal vesicles are shown in Table 4 and the number of sperm is shown in FIG.

Experimental Example 7 Measurement of Serum cGMP and Testosterone Levels in Rats

As the experimental animals, 10 male rats (ICR mice, 7 weeks old at the time of purchase) were used in each group, and were divided into a control group and a sexual improvement composition group.

Breeding is avoided as much stress as possible in a constant environment of room temperature 22 ㅁ 1 ℃, humidity 55 ㅁ 5%, light and dark cycle 12 hours, and divided into normal group and cadmium chloride group. The normal group is orally administered to the control group administered distilled water and the experimental group administered the 30 mg / mice / day to the sexual improvement composition once a day for 7 days each. The cadmium chloride group was orally administered once a day for 7 days to the control group administered distilled water and the experimental group administered 30 mg / mice / day to the sexual enhancement composition, and intraperitoneally administered once with 0.06 mg / mice of cadmium chloride after the last oral administration. My injection After 24 hours, mice were sacrificed and 1 ml of blood was isolated, allowed to stand at room temperature for 10 minutes, and then serum was separated.

CGMP concentrations in serum isolated were measured using the Enzyme Immunoassay Kit (Assay Designs, USA). Transfer 100 μl of the serum to a goat anti-mouse IgG microtiter plate and incubate for 2 hours with cGMP EIA antibody and conjugate and wash with washing solution. After washing, pNpp substrate solution was added to stop the reaction by adding a stop solution after 1 hour, and cGMP amount was calculated after absorbance measurement at 405 nm.

To determine testosterone concentrations, the serum was measured using an Enzyme Immunoassay Kit (Assay Designs, USA). Transfer 100 μl of serum to goat anti-mouse IgG microtiter plate, incubate for 1 hour with testosterone EIA antibody and incubate for 1 hour with conjugate. After washing, the solution was added to the pNpp substrate solution, and the reaction was stopped for 1 hour after the addition of the stop solution.

As shown in FIG. 5, the cGMP concentration of the group ingesting the sexual improvement composition was about 27.3% higher than that of the control group. In the group exposed to environmental hormone by administering cadmium chloride, it was confirmed that the cGMP reduction caused by cadmium chloride was also suppressed by ingesting the sexual improvement composition. Through Experimental Example 2 above, it can be considered that the concentration of cGMP was increased by inhibiting the activity of PDE (phosphodiesterase) involved in the degradation of cGMP.

In addition, as shown in Figure 6, the testosterone concentration of the group ingesting the sexual improvement composition was about 17.3% higher than the control group. In the group exposed to environmental hormone by the administration of cadmium chloride, the control group showed a testosterone concentration of about 41.5% lower than the group not receiving cadmium chloride, but the group treated with cadmium chloride after ingestion of the sexual enhancement composition did not receive cadmium chloride. It was confirmed that the testosterone concentration of the group was maintained similar to. These results show that ingestion of sexual improvement composition is effective in preventing sexual degradation by environmental hormones.

Experimental Example 8. Single oral administration toxicity test through animal experiment

In order to determine the toxicity of the sexual improvement composition of the present invention, a single oral dose toxicity test was performed using a test animal.

Experimental animals were male rats (SD rats, 4 weeks old at purchase) and 5 control groups and 10 sexual improvement composition groups, and magnetic rats (SD rats, 4 weeks old at purchase) were 5 controls, sexual controls were improved. Ten composition groups were used. It was bred so as to avoid possible stress in a constant environment of room temperature 22 ㅁ 1 ℃, humidity 55 ㅁ 5%, contrast cycle 12 hours. The control group of each group was orally administered with 1 ml of distilled water, and the sexual improvement composition group was orally administered by dissolving the sexual function improving composition in 1 ml of distilled water at a concentration of 3 g / kg. Two weeks after oral administration, the general health status including survival, weight, hair gloss, bristle, bowel movement, and overall movement was checked.

As a result of the experiment, all 20 experimental groups orally administered the sexual improvement composition survived and had a good state of health and no specificity was found (Table 5).

Experimental Example 9. Clinical Trial of Sexual Function Improvement Effect

To test the sexual function-improving effect of the sexual improvement composition, 10 adult male volunteers were recruited and 6 patients filled the 500 mg hard capsule with the sexual improvement composition twice a day for 2 capsules (1 g) each morning and evening. 4 control groups were filled with 500 mg hard capsules of placebo (dextrin) and ingested in the same manner. After 30 days, a survey was conducted to evaluate subjective symptoms.

Participating test subjects are shown in Table 6 below, and each subject was subjected to a double blind test (double blind test) that did not disclose whether the control group and sexual improvement composition group.

After the clinical trials, the scores were divided into seven domains of foot function, stiffness, sexual desire, satisfaction, sexual enhancement, duration, and side effects, and the scores were compared from 1 (no effect) to 5 (high effect). The results of the survey showed 42.3% higher scores in the sexual function composition group compared to the control group, and the stiffness was 46.8%, sexual desire was 34.9%, satisfaction 50.9%, sensation increase 36%, and duration 34.6% in all domains. It was evaluated higher than the control group (Fig. 7). In addition, all 10 subjects said they had no feeling about side effects such as nausea, dizziness and headache.

As described and demonstrated in detail above, the present invention is a sexual function improving composition comprising extract and fermentation extract of maca, red ginseng, namgasae having improved sexual function and increased sperm count and preventing male reproductive function by environmental hormones (aka MP119) To provide. Sexual improvement composition was found to inhibit ACE and PDE and increase serum cGMP content to induce erection through increased blood flow and relaxation of the penile smooth muscle, and improve sexual function by increasing sex hormone. Animal experiments confirmed that reproductive organs gain weight and sperm count, and that cadmium chloride prevents reproductive function from decreasing. As a result of clinical trials, it was confirmed that it can act as a composition that can effectively improve sexual function by receiving excellent scores in all domains such as stiffness, sexual desire, satisfaction, sexual enhancement and duration.

1 is a diagram showing the survival rate of mouse testis cells TM3 at various concentrations of the sexual function improving composition of the present invention.

Figure 2 is a measure of the testosterone concentration by the sexual function improving composition in TM3.

Figure 3 is a diagram showing the change in sperm count of the mice administered the sexual function improving composition.

Figure 4 is a diagram showing the change in sperm count of rats by environmental hormone after administering sexual improvement composition to cadmium chloride.

5 is a diagram showing the measurement of cGMP concentration in the serum of the mice administered the sexual function improving composition.

Figure 6 is a diagram showing the testosterone concentration measured.

Figure 7 is a diagram showing the results of clinical trials of sexual improvement composition.

Claims (8)

Maca extract, red ginseng extract, namgasae extract containing as an active ingredient, Maca extract of the extract is a sexual function improvement composition, characterized in that obtained by hot water extraction. The method of claim 1, Maca extract 30-70% by weight, red ginseng extract 40-60% by weight based on solids, sexual function improvement composition comprising 20-40% by weight. The method of claim 1, Sexual improvement composition, characterized in that it comprises fermenting one or more selected mixed extract of the extract crowd. The method of claim 3, A lactic acid bacteria (Lukeconstock, Lactobacillus, Lactococcus, Pediococcus, Bifidobacterium, Straptococcus) is added to fermentation alone or mixed starter composition. The method of claim 4, wherein The lactic acid bacteria are Leuconostoc citreum , Leuconostoc gelidium , Leuconostoc mesenteroides sub. Mesente roides , and Lactococcus lactis , Lactococcus lactis subsp.lactis , Lactococcus plantarum and Lactobacillus brevis in Lactococcus plantarum , Lactobacillus plantarum , Lactobacillus plantarum , and Lactobacillus sake . Lactobacillus bulgaricus ), Lactobacillus casei , Lactobacillus acidophilus and Streptocuccus thermophilus in the Straptococcus and Bifidobacterium spp. ( Bifidobacterium bifidum ), Bifidobacterium lacti s ), Bifidobacterium longum and finally lactic acid bacteria such as Pediococcus pentosaceus , Pediococcus massilacti in Pediococcus Sexual improvement composition, characterized in that. Maca extract, red ginseng extract, namgasae extract containing as an active ingredient, maca extract of the extract is a food for improving sexual function, characterized in that obtained by hot water extraction. Gum, tea, vitamin complex, health supplement foods, honey products, including the composition of claim 1 or 3 as a raw material for improving sexual function, characterized in that. The method of claim 7, wherein Food for improving sexual function, characterized in that the powder, granules, tablets, capsules, pills or liquids.

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