KR101579845B1 - Composition that comprising fenugreek extract or compound including fenugreek extract for preventing and improving the andropause or male menopause symptom which involves lethargy, hypotrophy, muscle weakness, increasing visceral fat, tiredness and stress, by increasing testosterone level, endurance, muscle mass and decreasing visceral fat and improving blood lipid composition, number of sperm and activation of sperm - Google Patents

Abstract

The present invention relates to a food and a pharmaceutical composition containing fenugreek, Lespedeza cuneata G. Don and Tribulus terrestris L. extracts, helpful to the health of men in andropause, as active ingredients. Particularly, in the present invention, provided is a composition containing any one among the fenugreek, the Lespedeza cuneata G. Don and the Tribulus terrestris L. extracts or a complex extract obtained by composing two or more thereof as an active ingredient which prevents or alleviates andropause symptoms, namely lethargy, hypotrophy, decrease in sexual desire and an erectile function, decrease in intelligence capability and spatial sensibility, tiredness, depression, mental anger, decrease in a muscle mass and muscular strength, decrease in hair, decrease in bone density, decrease in endurance, and increase in visceral fat; and has efficacies of increase in testosterone, endurance and muscular strength, decrease in body fat and blood lipid composition, and increase in a sperm count and sperm activation. The composition according to the present invention promotes secretion of testosterone to alleviate the andropause symptoms.

Description

To improve, prevent and treat menopausal symptoms such as male hormone increase, endurance and strength, increase in body fat and blood lipids, increase in sperm count and sperm motility, and decrease in vitality, vitality, muscle weakness, fatigue and fatigue. [0002] The present invention relates to a combination composition comprising fenugreek, fenugreek, fenugreek, or fenugreek extract for preventing and / or improving the orropause or male menopause symptom which comprises lethargy, hypotrophy, muscle weakness, increasing visceral fat, tiredness and stress, level, endurance, muscle mass and decreasing visceral fat and improved blood lipid composition, number of sperm and activation of sperm}

The present invention relates to a composition for preventing and treating male menopausal symptoms comprising, as an active ingredient, one or more extracts selected from the group consisting of fenugreek,

The scientific name of Trigonellae Semen is Trigonella foenum-graecum L., also called Fenugreek. Its origin is India, China, Southeast Asia, Europe, Egypt, Sudan, the United States, Lebanon, and Argenetin. Horopa is generally sun-dried and used medicinally. It is used to treat bladder and kidney diseases in ancient times in Africa, the Middle East, and India, and it is also used to treat cold sweaty or stomachful people. It is said that it is painful, kneeling and curing of the scrotum and it treats all 虛寒 证 (虚寒 证) and the symptom of the jelly, treating the symptom which frequent the urine and treating the cold of the stomach. Recently, it has been known that it is effective in maintaining balance between insulin and blood sugar in the body, and also has an effect on weight control. However, it has not yet been used to prevent and improve male menopausal symptoms.

The nightclub is also known as an apron, but its scientific name is Les Pedzaci Nitta. Don ( Lespedeza cuneata G. Don), and its tinnitus is 老 牛 (牛 牛), Hojija, and horses. Origin is known as Korea, Japan, Taiwan, India and Australia. The outposts of Lespedeza cuneata and Lespedeza daurica help preserve the kidney kidneys, help the lungs in the lungs, eliminate ejaculation, and calm the swelling. It is known as a medicinal herb that helps the kidneys and helps the asthma calm. In addition, it is known that it has the effects of Jinhae and scion. There is no evidence of a malignant bladder in a child's anemia, anemia, milk, or other symptoms of a child's illness, such as anorexia nervosa, urinary bladder, stomach pain, stomach cramps, coughing and asthma But it has not been used to prevent and improve male menopausal symptoms.

The scientific name of the Namgalbong is Tribulus terrestris L. Tribulus Fructus and Baekjil Lai. Origin is known as Korea, India, Taiwan and so on. It is said that it was used as a medicine because it was used as medicine. It is disturbed by hypertension and it is headache is severe. When taking it when it is depressed, blood pressure It is known to be effective in treating ophthalmic diseases in which the eyes are congested, the eyes are torn, the eyesight is diminished, and the cornea becomes cloudy. In addition, when the skin is itchy and the spot appears due to heartburn or neurogenic dermatitis, it is also used for the angina pectoris due to coronary artery insufficiency, and the caloric and hypotensive effect is also recognized in animal experiments. However, it has not yet been used to prevent and improve male menopausal symptoms.

Korean Patent Registration No. 10-576130 discloses a pharmaceutical composition for promoting the secretion of growth hormone by using an extract of a fenugreek as an active ingredient, and Korean Patent Registration No. 10-8448213 discloses a method for producing a fenugreek, Hormone secretion promoting action has been disclosed, and patent registration No. 10-1114499 discloses the efficacy of skin treatment for the skin diseases and the anti-aging effect of skin.

In the 4th and 5th decades, menopausal symptoms have emerged as a result of a decrease in male hormones (Werner AA, The male climateric. J Am Med Assoc 1939; 112: 1441-1443). It is known that the male hormone is decreased by about 1% per year since the age of about 30, and it decreases the sexual desire and erectile function, especially the nighttime erection, the decrease of intellectual ability and spatial perception, fatigue, depression, anger, There are various health problems such as decrease of muscle mass and muscle strength, decrease of body hair, decrease of bone density, increase of visceral fat.

According to a national survey, 64.6% of males in their 40s or older who complain of menopausal symptoms were reported, and more than half of Korean middle-aged men were suffering from menopause (Park MG et al., AIDS of Korean males toward late-onset hypogonadism: survey of males in their 40s and above 2007).

Current treatments for the improvement of so-called male menopausal symptoms are directed at supplementing male hormones directly via injections, oral and patch formulations. However, when using direct male hormone replacement therapy, side effects that exceed the acceptable levels in the body may result in excessive hormone levels. In addition, as the concentration of testosterone in the male blood increases, the blood becomes sticky and reduces the width of the blood vessels and obstructs the flow of blood, thereby increasing the risk of exposure to cerebral infarction or cardiovascular diseases, and increasing the size of the prostate gland, . In addition, sebaceous secretion is active, such as acne and skin disease can be adverse side effects.

There is therefore a continuing need for the development of effective natural product-based compositions that can maintain or maintain normal levels of male hormone without such side effects to prevent or ameliorate undesirable symptoms of male menopause.

Korean Patent Registration No. 10-576130 Korean Patent Registration No. 10-8448213 Korean Patent Registration No. 10-1114499

It is an object of the present invention to provide a novel natural ingredient having an effect of preventing and ameliorating male menopausal symptoms. More specifically, the present invention provides a method for improving menopausal symptoms by enhancing male sex hormones, which is effective for improving and treating menopausal symptoms. And a composition for preventing or treating male menopausal symptoms, which comprises, as an active ingredient, any one of the extracts selected from the group consisting of insecticide extracts and camphor tree extracts, or a mixture of two or more extracts.

The composition according to the present invention may be any one of the extracts selected from the group consisting of fenugreek, fenugreek, and scallion, or a mixture of two or more extracts.

According to the present invention, the fenugreek, the gazebo and the southern stench extract can be extracted by a known natural material extraction method. Each material before the extraction, that is, the fenugreek, the yuan gate and the southern stenra are collected, cleaned and crushed.

The extraction solvent may preferably be extracted with water, at least one solvent selected from C ₁ to C ₆ organic solvents, and the C ₁ to C ₆ organic solvent may be C ₁ to C ₆ alcohol, acetone such as acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane, cyclohexane, and petroleum ether, ≪ / RTI >

The solvent used in the preparation of the extract of the present invention may more preferably be water, a C ₁ to C ₆ alcohol or a mixture thereof, and most preferably, the alcohol is ethanol. Most preferably, 60-80% ethanol can be used by mixing water and ethanol.

The amount of the solvent may be varied depending on the amount of the holofa, the nightstand and the southern grayfin material to be extracted, preferably 3 to 10 times the total weight of the material, and when 60 to 80% ethanol is used as the extraction solvent Most preferably from 8 times to 10 times the volume.

The extraction temperature of the extract of the present invention is not particularly limited, but may be preferably 50 ° C to 100 ° C, and most preferably 60-80 ° C when 60-80% ethanol is used.

The extraction time of the extract of the present invention is not particularly limited, but may be 2 hours to 6 hours, and most preferably 3-4 hours when 60-80% ethanol is used.

The extract of the present invention can be extracted by a known natural substance extraction method. For example, conventional extraction methods such as supercritical extraction, subcritical extraction, high-temperature extraction, high-pressure extraction, ultrasonic extraction, or using adsorbent resins including XAD and HP-20 can be used And preferably, it can be extracted by heating extraction or reflux extraction. The extraction step of the present invention can be repeated one to seven times, preferably two to seven times, and when 60 to 80% ethanol is used, it is most preferable to repeatedly extract one to three times.

For example, the extract according to the present invention may be prepared by adding a pulverized raw material such as fenugreek, a nightclub or a southern slug, into an extraction container equipped with a reflux condenser, adding an extraction solvent having a volume of 3 to 10 times the weight of the powder, boiling the solution, After extraction, it is allowed to stand for a certain time and then filtered with a filter paper or the like to obtain an alcoholic extract. It is preferable that the extraction time is 2 to 6 hours. After the concentration and sterilization step, the extract can be used as a liquid phase after the concentration and sterilization step. In the present invention, It is preferable to use it in the form of a mixture.

The fenugreek extract, the yambaekisan extract and the southern stingray extract of the present invention may further be subjected to a fermentation process for the pulverized material before the extraction process, or the extract after filtration and concentration after the extraction. Through the fermentation process, the effect of the extract of the present invention on the improvement of male menopausal symptoms can be further increased. The fermentation process of the extract of the present invention can be carried out by a known fermentation method. The fermentation strain is not particularly limited, but it may be lactic acid bacterium, preferably Bifidobacterium longum ) or Lactobacillus acidophilus ( Lactobacillus acidophilus , Lactobacillus bulgaricus ) or Streptococcus thermophilus ( Sterptococcus thermophilus) or Bifidobacterium Infante Tees (Bifidobacterium infantis) or Lactobacillus casei (Lactobacillus casei) or Lactobacillus Planta Room (Lactobacillus Plantarum ) or a mixed strain of these lactic acid bacteria. The fermentation conditions may vary depending on the type of strain used.

The composition of the present invention is characterized by being a composition containing, as an active ingredient, any one of extracts selected from the group consisting of fenugreek, fenugreek, and scallop, or mixtures of two or more extracts. And a blend of camphor tea extract and wild camphor tea extract, a blend of fenugreek extract and southern fungus extract, a mixture of camphor tea extract and southern fungus extract, a fenugreek extract, a yamba gum extract and a southern fungus extract. The amount of each extract may be selected according to the number of cases at 0.1 to 99.9% by weight, but preferably, when three kinds of extracts are mixed, 30 to 40 parts by weight of the artificial door, 30 to 40 parts by weight of the male shorebird, 45 to 55 parts by weight of fenugreek, 10 to 20 parts by weight of the tentacle door, and 25 to 35 parts by weight of the shorelines, Can be 1: 1: 1, 2: 1: 1, 2: 1: 2 or 3: 1: 2 by weight.

The extracts of the fenugreek, the gazebo and the southern grayfin tusk and their mixtures are effective in increasing male hormones.

Accordingly, the present invention provides a food composition for preventing and ameliorating a male climacteric-related disease or symptom, which comprises, as an active ingredient, any one of extracts selected from the group consisting of fenugreek, fenugreek, and clam; .

The present invention also provides a pharmaceutical composition for the prevention and treatment of male menopausal symptoms or symptoms comprising an extract of any one selected from the group consisting of fenugreek, fenugreek, and clam; do.

The food composition of the present invention includes all forms of natural materials such as foods, functional foods, nutritional supplements, health foods and food additives. Food compositions of this type may be prepared in a variety of forms according to conventional methods known in the art. For example, they may be prepared in the form of tea, juice, extract pouches, and drinks, and may be ingested, granulated, encapsulated and powdered.

In addition, the extract and extract of the present invention can be mixed with a known active ingredient known to have an effect of preventing or ameliorating a disease or symptom caused by menopausal period, thereby preparing the composition in the form of a composition.

In order to use the extract and extract of the present invention in the form of a food additive, they may be prepared in the form of liquid or powder. When used as a food additive, it may be added in an amount of 0.01 to 10% by weight, preferably 0.1 to 5% by weight based on the raw material. The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment). However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of stability, the active ingredient may be used in an amount exceeding the above range. There is no particular limitation on the kind of the food. Examples of the food to which the extract can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, , An alcoholic beverage and a vitamin complex, and includes all the health foods in a conventional sense.

In addition to the above, the composition according to the present invention may further comprise various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid concentrating agents, pH adjusting agents, stabilizers, Alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the composition according to the invention may contain flesh for the production of natural fruit juices and vegetable drinks. These components can be used independently or in combination, and the proportion of the additives is not critical, but is preferably 30% by weight or less per 100 parts by weight of the composition according to the present invention.

A pharmaceutical composition for preventing or treating male menopausal symptoms comprising an effective ingredient selected from the group consisting of the extracts of fenugreek, fenugreek extract, and southern poplar extract according to the present invention, or a mixture of two or more extracts, The composition can be formulated into parenteral administration forms, but is not limited thereto.

Examples of formulations for oral administration include tablets, pills, light / soft capsules, liquids, suspensions, emulsions, syrups, granules and elixirs. These formulations include fillers, extenders, A wetting agent, a disintegrant, a lubricant, a binder, a surfactant, and the like may be used. As the disintegrant, agar, starch, alginic acid or its sodium salt, anhydrous calcium monohydrogenphosphate, etc. may be used. As the lubricant, silica, talc, stearic acid or its magnesium salt or calcium salt, polyethylene glycol and the like may be used , Magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose, polyvinylpyrrolidine, and low-substituted hydroxypropylcellulose can be used as the binding agent. In addition, lactose, dextrose, sucrose, mannitol, sorbitol, cellulose, glycine and the like can be used as a diluent. In general, a commonly known boiling mixture, an absorbent, a colorant, a flavoring agent and a sweetening agent can be used together.

Also, a pharmaceutical composition for preventing or treating cancer, which contains, as an active ingredient, any one extract or any combination of two or more extracts selected from the group consisting of fenugreek extract, yambaekisan extract, and clove extract can be administered parenterally, Is by injecting subcutaneous injection, intravenous injection, intramuscular injection or intra-thoracic injection. At this time, in order to formulate the preparation for parenteral administration, any one of extracts selected from the group consisting of fenugreek extract, yambaekisan extract and southern stover extract or a mixture of two or more extracts is mixed with water in a stabilizer or a buffer to prepare a solution or suspension And can be prepared in unit dosage form of ampoules or vials.

The composition may be sterilized or contain adjuvants such as preservatives, stabilizers, wettable or emulsifying accelerators, salts for controlling osmotic pressure, buffering agents and other therapeutically useful substances and may be prepared by conventional methods such as mixing, granulating or coating Or the like.

When a pharmaceutical composition for prevention or treatment of cancer comprising, as an active ingredient, any one of the extracts selected from the group consisting of the fenugreek extract, the yamaguchi extract, and the southern stover extract of the present invention or a mixture of two or more extracts is formulated in unit dosage form As an active ingredient, any one of the extracts selected from the group consisting of fenugreek, fenugreek, and scallop extract or a mixture of two or more extracts is preferably contained in a unit dose of about 0.1 to 20 mg. The dosage depends on the physician's prescription depending on factors such as the patient's weight, age and the particular nature and severity of the disease. However, the dosage required for adult therapy is usually about 1 to 100 mg per day, depending on the frequency and intensity of administration.

By applying the food and medicament containing the extract and the mixture of the extract of the present invention, it is possible to prevent the occurrence of male menopausal symptoms of a side effect-free ingredient, to delay the time of occurrence of the menopausal symptom, and to achieve epoch-making effects in relieving and treating symptoms.

FIG. 1 is a table comparing the increase in the secretion amount of testosterone when each of the fenugreek extract,
FIG. 2 is a chart comparing the increase of testosterone secretion amount when a mixture of a fenugreek extract, a yamaguchi extract, and a southern fungus extract is added.

The present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, and the present invention is not limited by the following examples.

Example 1 <Preparation of fenugreek extract>

100 g of clean fenugreek was prepared, and the fenugreek powder was pulverized to a size of 20mesh using a pulverizer, and 80% ethanol was extracted with 800 ml of the fenugreek powder at a temperature of 60 to 80 ° C for 3 hours. The extraction step was repeated twice under the same conditions. The ethanol extract was filtered with a filter, and the resulting filtrate was concentrated, sterilized at 95 ° C for 1 hour, and completely dried using a spray dryer to prepare powder, which was then prepared.

Example 2 < Preparation of a yam door extract >

100 g of clean night trays were prepared and milled to a size of 20 mesh using a pulverizer, and 80% ethanol was extracted with 800 ml at a temperature of 60 to 80 ° C for 3 hours in the pulverized whisker door powder. The extraction step was repeated twice under the same conditions. The ethanol extract was filtered with a filter, and the resulting filtrate was concentrated, sterilized at 95 ° C for 1 hour, and then completely dried using a spray dryer to prepare a powdery yam cabbage extract.

&Lt; Example 3 >&lt; Preparation of southern stevioside extract &

100 g of clean sharkbirds were prepared and milled to a size of 20 meshes using a pulverizer. The 80% ethanol was extracted with 800 ml of the powder at 60 to 80 ° C for 3 hours in the pulverized shalebird powder. The extraction step was repeated twice under the same conditions. The ethanol extract was filtered with a filter, and the resulting filtrate was concentrated, sterilized at 95 ° C for 1 hour, and dried thoroughly using a spray dryer to prepare a powder of Nambalai.

Preparation Example 1 Preparation of a 1: 1: 1 mixture (H): Horseradish extract (Y): Scutellariae Radix extract (B)
Each of the extract powders prepared in Examples 1 to 3 was mixed with 33.33 parts by weight of fenugreek, 33.33 parts by weight of yamabori, and 33.34 parts by weight of scabbard, among 100 parts by weight of the total mixture.

Preparation Example 2 Preparation of a 2: 1: 1 mixture (H): Horseradish extract (Y): Scutellariae Extract (B)

Each of the extract powders prepared in Examples 1 to 3 was mixed with 50 parts by weight of fenugreek, 25 parts by weight of the yam door, and 25 parts by weight of male scutellum among 100 parts by weight of the total mixture.

Preparation Example 3 <Preparation of Holo wave extract (H): yamgwanmun extract (Y): S. sinensis extract (B) Preparation of 2: 1: 2 mixture>

Each of the extract powders prepared in Examples 1 to 3 was mixed with 40 parts by weight of fenugreek, 20 parts by weight of a night scarecrow, and 40 parts by weight of male scabbard among 100 parts by weight of the total of the mixed powders.

Preparation Example 4 <Preparation of Holo wave extract (H): Yambu Kannon extract (Y): Namulga extract (B) 3: 1: 2 mixture>

Each of the extract powders prepared in Examples 1 to 3 was mixed with 100 parts by weight of the mixture, with 50 parts by weight of fenugreek, 16.7 parts by weight of the yam door, and 33.3 parts by weight of scarlet mane.

Experimental Example  One

TM3 cells (mouse leydig cells, testosterone producing cells) were cultured overnight in the medium, and then the extracts obtained in Examples 1 to 3 were treated with cells and cultured for 2 days. The cultured cells were harvested and testosterone levels were measured using Testosterone ELISA kit. The results are shown in Fig.

As a result, the secretory amount of testosterone increased to 25 pg / ml in the cell treated with fenugreek extract, 12.5 pg / ml in the cell treated with scarlet extract, All tested at the level of 7.5 pg / ml compared to untreated cells.

Experimental Example 2

TM3 cells (mouse leydig cells, testosterone producing cells) were cultured overnight in a medium, and the mixture of each of the extracts obtained in Production Examples 1 to 4 was treated in a cell and cultured for 2 days. The cultured cells were collected and testosterone concentration was measured using a testosterone ELISA kit. The results are shown in FIG.

As a result, the amount of testosterone in the original TM3 cells not treated with the extract was 5 pg / ml, the highest value was 27.3 pg / ml in the cells treated with the extract according to Preparation Example 1, The highest increase was observed in 16.5 pg / ml of cells treated with the extract.

Experimental Example  3

In the same manner as in Experimental Example 2, each of the mixtures obtained by mixing the fenugreek extracts of Example 1 and 2 in a weight ratio of 8: 2, 6: 4, 5: 5, 2: 7 and 0.5: 9.5 The result of measuring the testosterone concentration of the resultant product after the treatment was shown in Fig.

As a result, the amount of testosterone in the TM3 cells not treated with the extract was 5 pg / ml, the highest 30 pg / ml in the 8: 2 mixture, and 17 pg / ml in the 0.5: 9.5 mixture.

Experimental Example  4

In the same manner as in Experimental Example 2, each of the mixtures obtained by mixing the fenugreek extracts of Example 1 and 3 in a weight ratio of 8: 2, 6: 4, 5: 5, 2: 7 and 0.5: 9.5 The result of measuring the testosterone concentration of the resultant product after the treatment was shown in Fig.

As a result, the amount of testosterone in the TM3 cells not treated with the extract was 5 pg / ml, the highest value of 28 pg / ml was observed in the 5: 5 mixture, and the amount of secretion was about 17 pg / ml in the mixture of 0.5: 9.5.

Experimental Example  5

In the same manner as in Experimental Example 2, each of the mixture of the yam Kanto extract prepared in Examples 2 and 3 at a weight ratio of 8: 2, 6: 4, 5: 5, 2: 7 and 0.5: 9.5 was treated The result of measuring the testosterone concentration of the resultant cultured product is shown in FIG.

As a result, the amount of testosterone in the TM3 cells not treated with the extract was 5 pg / ml, the highest 20 pg / ml value was observed in the 5: 5 mixture, and the secretion amount of about 15 pg / ml in the mixture of 8: 2 and 0.5: Respectively.

As shown in Experimental Examples 1 to 5, when the mixture of one or more of the fenugreek extract, the yambaekisan extract and the clover extract was selected, the amount of testosterone hormone secretion was remarkably increased compared to the untreated case regardless of the mixing ratio.

Experimental Example  6: Animal test: male hormone increase

1) Experimental conditions

Experimental animal

Twenty-four male Sprague-Dawley rats weighing 300 g were prepared.

Control group: Eight dogs

Experimental group (H): Eight dogs fed the fenugreek extract at 40 mg / kg / day in the morning

Experimental group (H + Y): A composition containing a mixture of a fenugreek extract and a yam Kwan Kwan extract was inoculated at a dose of 40 mg / kg /

Breeding condition

Temperature was 22 ± 2 ℃ and relative humidity was 50 ± 5%. The darkness and the darkness were automatically regulated at intervals of 12 hours. During the breeding period of 1 month, solid feed and distilled water were freely consumed.

2) Experimental results

The control and experimental groups were sacrificed for 1 month with CO ₂ , blood was collected from the heart, and testosterone concentration in blood was measured using Testosterone ELISA kit.

As a result, the test group H and the test group H + Y showed an increase in blood testosterone levels at a concentration of 59 pg / ml and 84 pg / ml, respectively, as compared with the control group. This indicates that the amount of testosterone in the blood was increased by 21% and 30%, respectively, as compared with the control ( FIG. 6 ).

Experimental Example  7: Animal experiment: Reduction of body fat and increase of muscle mass

1) Experimental conditions

Control and experimental groups were prepared and maintained under the same conditions as Experimental Example 6 above.

2) Experimental results

The control group and the experimental group were sacrificed for 1 month, CO ₂ was sacrificed for 1 month, and the external light chain and epididymal fat of both hind limbs were excised, washed with physiological saline, and then weighed using microbalance.

As a result, in the experimental group H and the experimental group H + Y, the body fat amount was decreased by 20% and 33%, respectively ( FIG. 7 ) and the muscle mass was increased by 17% and 47%, respectively ( FIG .

Experimental Example  8: Animal Test: Endurance Increase

1) Experimental conditions

Control and experimental groups were prepared and maintained under the same conditions as Experimental Example 6 above.

2) Experimental results

The control and experimental groups were forced to swim in aquariums for a total of 4 times a week while breeding for 1 month under the respective conditions. The sterilized water was filled up to a height of 70% in a plastic water tank, and the water temperature of 25 ± 2 ° C was maintained using an underwater heater. In the aquarium, a forced swimming exercise was performed until the time when the nose of the experimental rat sank below the water surface for 5 seconds or more (exhaustion), and the maximum swimming performance was measured.

As a result, compared to the control group, the change in swimming time of the experimental group H and that of the experimental group H + Y were increased by 107 and 90 seconds, respectively, compared to that of the control group by 47 seconds, Compared to the control group ( Fig. 9 ).

Experimental Example  9: Animal experiment: blood lipid reduction

1) Experimental conditions

Control and experimental groups were prepared and maintained under the same conditions as Experimental Example 6 above.

2) Experimental results

The control and experimental groups were fed for 1 month, then sacrificed by CO ₂ , blood was collected from the heart, and serum total cholesterol, HDL - The contents of cholesterol and triglyceride were measured.

As a result, the total cholesterol in the experimental group H and the experimental group H + Y was decreased by 18 mg / dL and 27 mg / dL, respectively, compared with the control group, and the HDL cholesterol was increased by 3 mg / dL and 8 mg / dL, / dL, 83 mg / dL. Thus, it was confirmed that the combination of the Herba extract and the Holofa / Busanum composite extract reduced the total cholesterol and triglyceride in blood and increased the HDL level, which is called good cholesterol, to improve the lipid in the blood ( FIG. 10 ).

Experimental Example  9: Animal experiments: Changes in sperm count

1) Experimental conditions

Control and experimental groups were prepared and maintained under the same conditions as Experimental Example 6 above.

2) Experimental results

The control and experimental groups were fed for 1 month, sacrificed by CO ₂ , and the testes were extirpated. All the epididymis and the posterior part were ligated and extracted with a hemostatic forceps. The sperm was collected and placed on a slide glass heated to 37 ° C. The number of spermatozoa was counted using a microscope, and the motility of the live sperm was observed at intervals of 15 minutes for 3 hours.

As a result, the number of total sperm in the experimental group H and the experimental group H + Y was increased by 7.4% and 15.9%, respectively, compared with the control group, and the number of motile sperm was significantly increased to 60.6% and 84.6% ( Fig. 11 ), as well as the motility of the sperm.

According to the present invention, by providing a composition for preventing or treating male menopausal symptoms containing a novel natural component having an effect of preventing and ameliorating male menopausal symptoms, it will make a significant contribution to the health food sector and the pharmaceutical industry related to menopause It is expected.

Y: Nightclub
B:
H: Horopa

Claims (10)

A composition for preventing or treating male menopausal symptoms comprising an 8: 2 weight ratio mixture of a fenugreek extract and a yamaguchi extract as an active ingredient.
A composition for preventing or treating male menopausal symptoms, comprising as an active ingredient, a 5: 5 weight ratio mixture of a yam Kwan Mun extract and a southern bluefin extract. Preventing or treating male menopausal symptoms containing a mixture of fenugreek, fenugreek, and clove extracts in a weight ratio of 1: 1: 1, 2: 1: 1, 2: 1: 2 or 3: 1: 2 / RTI &gt;
delete delete delete delete delete 4. The method according to any one of claims 1 to 3,
Crushing each of the fenugreek, the nightclub and the baguette;
Extracting the pulverized product at a temperature of 60-80 ° C for 3-4 hours using 60-80% ethanol as an extraction solvent at a volume of 8-10 times the volume;
Repeating the extraction one to three times under the same condition as the extraction step;
Filtering the ethanol extract;
Concentrating and pasteurizing the filtrate; And
And a step of drying and pulverizing the sterilized concentrated liquid to obtain a composition for preventing or treating male menopausal symptoms.
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