KR20100120545A - Composition for improving hair growth - Google Patents

Abstract

PURPOSE: A composition for preventing hair loss and promoting hair growth is provided to suppress TGF-beta 1 and to promote hair follicle cell activation. CONSTITUTION: A composition for preventing hair loss and promoting hair growth contains extract obtained by filtering independent low molecular material, ion, ash impurity using an ultrafiltration membrane. The composition further contains Saussurea lappa, Lycium chinense, Prunella vulgaris var.lilacina or Kochia scoparia. A formulation for topical application on the scalp contains the composition.

Description

 Hair growth promoter composition {Composition for improving hair growth}

The present invention relates to a hair loss prevention and hair growth promoter composition containing a herbal medicine selected from the group consisting of Mokhyang, Gugija, hachicho and branch, more specifically the present invention is 5,000 to 300,000 Daltons after extraction with ultrasound The extract filtered using a polymer ultrafiltration membrane with a molecular weight cut-off of about the same time (dalton) shortens the cycle from the resting phase to the growth phase of the hair growth cycle. It is related to a hair growth promoter having excellent hair follicle cell activity, along with a hair growth phase induction-promoting effect of growing fast.

Human hair is about 100,000 to 150,000 pieces, each hair has a different cycle and grow, drop out through the anagen, catagen, telogen. This cycle is repeated over three to six years, resulting in an average of 50 to 100 hairs falling out per day. Generally, the term alopecia refers to the increase in the number of hairs that fall abnormally due to shortening of the hair growth rate in the growth phase and more hair in the degenerative or resting phase.

The causes of alopecia have been discussed, such as excessive male hormone action, excessive sebum secretion, poor blood circulation, hypoglycemia caused by peroxide or bacteria, genetic factors, aging, stress. However, to date, no clear cause for hair loss is unknown, and it is known that a very complicated action is intertwined.

The various hair loss prevention products known to date are effective ingredients for promoting blood circulation, strengthening the hair root function, moisturizing the scalp, preventing dandruff, antioxidant effects, prolonging the hair growth period, and inhibiting male hormone activity. It contains ingredients, etc., but there is still no clear effect in terms of effects and problems of side effects have been raised.

As an experimental method used to prevent hair loss and promote hair growth, in vivo evaluation using experimental animals and in vitro evaluation using hair follicle cells and tissue culture are mainly used. In the 1980s, research on the differentiation mechanisms in hair follicles was invigorated by the development of culture methods for dermal papilla cells and outer root sheath cells, which are the core cells of hair follicles. With the development of tissue culture methods, it has been shown to be a useful evaluation model for research on hair growth, wool and hair loss prevention. In recent years, biochemical and molecular biological experiments have been actively conducted to identify various growth factors and genes related to hair growth, research on mechanism of action, and drug search.

The effects of a commercially available product on the hair as a hair growth or hair loss inhibitor on the hair include growth phase induction effect, hair growth phase extension effect, 5a-reductase inhibitory effect, blood circulation promoting effect, bactericidal effect, anti-dandruff effect, moisturizing effect, and antioxidant effect. And the like, but the effect of preventing hair loss and promoting hair growth is not sufficient in existing formulations.

Androgenetic alopecia is directly dependent on the amount of male hormone since it is dependent on male hormones, and thus, many studies have recently been reported to prevent and treat hair loss through inhibition of male hormone activity. Briefly explain the mechanism of hair loss on male hormones are as follows. In other words, testosterone, one of the male hormones, is converted to dihydrotestosterone, an active male hormone, by an enzyme called 5α-reductase, and this activated hormone binds to a specific receptor to cause a hair loss protein. Induces hair loss. In addition, this mechanism may cause excessive production of sebum, causing acne, seborrheic dermatitis, and the like, and as a result, scalp may occur with inflammation. As a result, androgenetic alopecia is due to the excessive production of dihydrotestosterone by the action of 5α-reductase, it will be able to fundamentally effectively prevent and treat male alopecia if inhibiting the activity of 5α-reductase. In view of this, male hair loss treatment using finasteride, which has been used as a prostate treatment for men, has been developed, but side effects such as sexual dysfunction have been reported, and their 5α-reductase activity against hair loss It is also difficult to fully anticipate the effect by inhibitory effect alone.

Recently, in the field of hair, the use of herbal medicines is prominent, and it has been reported that the dandruff relieving effect is excellent, and in particular, in terms of hair growth promoting effect, there have been many studies on hair loss inhibiting and hair growth promoting compositions using herbal extracts.

However, the herbal compositions using the herbal extracts extracted by conventional methods did not have high practicality because the desired hair growth promoting effect was insignificant.

On the other hand, the extract using the ultrasonic technology is extracted by using ultrasonic waves at low temperature, significantly increasing the extraction rate of the active ingredient content of flavonoids, vitamins, polyphenols, etc., which is unstable to heat, and also prevents skin allergies due to impurities such as starch, rubber, ash, protein and sugar. It can be removed using an ultrafiltration membrane to obtain a safer substance for the skin.

In view of the above problems, the present inventors have examined dozens of herbal medicine extracts through experiments that can confirm the growth-induction promoting action of hair among the effects of various mechanisms using extracts purified using polymer ultrafiltration membranes after ultrasonic extraction. As a result of cleaning, it was found that extracts such as Mok-Gyu, Goji, Hachicho and Branch were promoted the transition from the resting phase to the growing stage of the hair growth cycle when new hair grows, grows and falls out again. In vitro evaluations showed very good results.

In order to solve the problems of the prior art, the present invention is non-irritating and safe to the skin through the extraction and purification technology using ultrasonic and ultrafiltration membranes, there is no problem of causing skin diseases, and has excellent hair growth promotion and hair loss prevention effect It is to provide a hair growth promoter composition.

The present invention relates to a hair loss inhibiting and hair growth promoting composition, and more particularly, excellent hair follicle cell activity and the effect of inhibiting the transformation promoter beta 1 from the herbal medicine extract purified using a polymer ultrafiltration membrane after ultrasonic extraction. And to provide a hair loss inhibition and hair growth promoting composition excellent in hair growth promoting effect by animal experiments.

In the effect test example below, the C57BL / 6 mouse model was used as an animal model for evaluating hair growth induction promoting effects. The C57BL / 6 mouse has black hair and is characterized by spontaneous alopecia. In addition, melanocytes are present only in the hair follicles, and melanin synthesis matches well with the hair growth cycle. It is widely used for research.

Comparative example  One. Hydrothermal  Extraction method

      After drying and cutting Mok-hyang, wolfberry, vinegar and branch, extracts were mixed in the same amount, and 400 mL of extraction solvent (distilled water: 1,3-butylene glycol = 7: 3) was added and the water bath (DWP-5000, After extracting for 4 hours while boiling in Daewoong tangtang) filtered to 0.2㎛ filter to obtain a final extract.

Comparative example  2. Ultrasonic Extraction Method

      After drying and cutting Mok-hyang, wolfberry, vinegar and branch, extracts were mixed in the same amount, 400 mL of extraction solvent (distilled water: 1,3-butylene glycol = 7: 3) was added, and the ultrasonic extractor (SM30) was added at room temperature. -CE, Sony Medi) 4 hours after the extraction was filtered with a 0.2㎛ filter so as not to produce a precipitate to obtain a final extract.

Example  1. Polymer after ultrasonic extraction Ultrafiltration membrane  How to Filtrate Through

      After drying and cutting Mok-hyang, wolfberry, vinegar and branch, extracts were mixed in the same amount, 400 mL of extraction solvent (distilled water: 1,3-butylene glycol = 7: 3) was added, and the ultrasonic extractor (SM30) was added at room temperature. -CE, SonyMedi) 4 hours after extraction and passed through a cartridge-type pretreatment filter of 1 to 50 micrometers size, and then filtered using an ultrafiltration membrane made of a polymer having a molecular weight of 10,000 to 200,000 Daltons An extract was obtained.

Experimental Example  1. Evaluation of hair growth induction promoting action

In order to determine the hair growth promoting effect of the herbal extracts prepared in Examples 1, 2, 3 were subjected to hair growth phase induction experiment. 42-56 days old mice (C57BL / 6, male), which are widely used to evaluate hair growth induction promoting effect, were used. First, the hair on the back of the mouse was removed using an electric razor, and each mouse was weighed and divided into 12 pieces so that the weight was evenly distributed. After one day of adaptation, the control and herbal extracts were applied to the hair removal site from the next day. At this time, 40% ethanol was used as a reference drug, and each herbal extract prepared in Examples 1,2 and 3 was used as a test drug. 100 uL was applied to the back of each mouse once a day for 28 days to observe the growth-induction promoting effect. The results were obtained by using an image analyzer to determine the ratio of the area where the newborn hair was grown to the area from which hair was removed. It was. Table 1 below shows the results of the hair growth phase induction promoting action of the medicinal herb extract, the effect according to the concentration.

extract density(%) Area ratio (%) Comparative Example 1 0.001 10.7 0.01 21.2 0.1 25.1 One 35.5 10 50.1 Comparative Example 2 0.001 21.0 0.01 33.9 0.1 52.6 One 68.2 10 79.5 Example 1 0.001 31.5 0.01 43.0 0.1 76.6 One 88.7 10 92.8 Control 5.2

As can be seen from the results, it was found that the herbal extracts filtered by the ultrafiltration membrane of the polymer after ultrasonic extraction showed a very good hair growth induction promoting effect in a concentration-dependent manner, and the hair growth promoting effect was excellent.

Experimental Example  2. Hair follicle  Activity increase assessment

In order to measure the hair follicle cell activity of the herbal extracts prepared in Examples 1, 2, and 3 above, human scalp tissues were obtained from scalp surgery and the hair follicle tissues were separated therefrom. First, the scalp tissue was sterilized by soaking in 70% ethanol for 1 minute and washed three times with DMEM, a cell culture medium. Under the stereo microscope, the epidermis and the dermis were cut out with a surgical scalpel to separate the epidermis, and hair follicles embedded in the dermis were pulled out one by one using tweezers. The separated hair follicles were transferred to DMEM culture medium containing 10% FBS (Fetal Bovin Serum), and the lower part of the hair follicles containing the dermal papilla was cut with a scalpel. Nipples were separated using fine tweezers and transferred to DMEM medium containing 20% FBS and incubated for 2 weeks. When cells grew from the dermal papilla tissue and filled with the culture vessel, the cells were separated from the culture vessel with 0.25% trypsin and 0.02% EDTA, followed by subculture. 2 ~ 3 passages of dermal papilla cells were diluted with DMEM medium containing 10% FBS at a concentration of 2 X 104 / mL, and then 5mL each of them were placed in a 60 mm culture vessel and incubated in a 37 ° C. incubator for 24 hours. The next day, remove the culture medium containing FBS, exchange it with the culture medium containing no FBS and the medicinal herb extract and incubate for 24 hours, and then add radiation-labeled thymidine (10 uCi) per plate. The time was incubated further. The cultured cells were washed three times with phosphate buffer and washed once with 5% trichloroacetic acid. Cells were dissolved in 0.1% sodium dodecyl sulfate and 0.5 N sodium hydroxide solution, mixed in a scintillation cocktail, and the amount of radioisotope was measured using a beta-counter. Cell activity was evaluated by comparing how much uptake of radiolabeled thymidine into the cells.

extract density(%) Cell proliferation activity (%) Comparative Example 1 0.000001 88.2 0.00001 94.6 0.0001 101.6 Comparative Example 2 0.000001 101.9 0.00001 114.6 0.0001 122.6 Example 1 0.000001 111.9 0.00001 130.6 0.0001 153.6 Control 100

As can be seen from the results in Table 2, the herbal extracts filtered by the ultrafiltration membrane of the polymer after ultrasonic extraction showed direct hair growth effect by acting on the hair follicle epithelium.

Experimental Example  3. Cytotoxicity Assessment

Method of MTT test by culturing V79-4 cells (Chinese hamster, lung tissue fibroblasts continuous cell line) with respect to the herbal extracts of Experimental Examples 1,2,3 (Mossman TJ Immunol. Meth. 55-63 (1983 )) Was performed to test the cytotoxicity, and the results are shown in Table 3 below. At this time, SLS was used as a control.

sample IC50 (%, w / v) SLS 0.005 Comparative Example 1 1.00 Comparative Example 2 5.00 Example 1 10.00

As can be seen in Table 3, the IC50 value of the medicinal herb extract filtered by the polymer ultrafiltration membrane after the ultrasonic extraction was 10%, which was two times lower than the SLS, indicating that the cytotoxicity was excellent.

Experimental Example  4. allergy  evaluation

In order to confirm the allergy of the herbal extracts filtered by the polymer ultrafiltration membranes after the ultrasonic extraction prepared in the above example, the experimental method using ethanol was selected as a carrier medium [Reference: Kimber I (1990): Identification of contact allergens using the murine local lymph node assay, J. Appl. Toxicol. 10 (3); 173-180].

First, as in the above embodiment, after extracting the ultrasonic medicinal herb extract filtered with a polymer ultrafiltration membrane was prepared in 20.0% by weight, 40.00% by weight and 60.00% by weight solution. That is, 50 μl was applied to both ears of the mouse (Balb / c) for 3 days, and then auricular lymph nodes were isolated from the mice. Milling the lymph nodes by a radioactive isotope of ^[3 H] and then made into a single cell - from the addition of methyl thymidine ^([3 H] -methyl thymidine) and then incubated for 24 hours, β- scintillation counter (Beckman LS 6000 TA, USA) to measure the degree of amplification (dpm, disintegrations per minute) of the cells, and the results are shown in Table 4 below. At this time, ethanol 60% solution was used as a control.

sample Lymph node count
(10 ⁷ cells / mL) Cmp
(Average) Amplification ethanol 2.13 2481 Transportation Herbal Medicine Extract Filtered by Ultrafiltration Membrane after Ultrasonic Extraction 1.87 1530 0.6 Herbal Medicine Extract Filtered by Ultrafiltration Membrane after Ultrasonic Extraction 1.88 2337 0.9 Herbal Medicine Extract Filtered by Ultrafiltration Membrane after Ultrasonic Extraction 1.87 2336 0.9

      The allergy evaluation result indicates the lymphocyte amplification of the sample group compared to the control group, and if the amplification degree (SI) of the sample is 3 or more at any concentration and shows a tendency to increase in a concentration-dependent manner, it is regarded as an allergen. As described above, the herbal extracts filtered by the ultrafiltration membrane of the polymer after the ultrasonic extraction used in the present invention showed that the amplification degree was 3 or less and there was no possibility of allergy.

The hair growth promoting composition filtered by the ultrafiltration membrane of the polymer after the ultrasonic extraction according to the present invention was far superior to the conventional hydrothermal extraction or the ultrasonic extraction and promoted the hair growth phase induction and hair follicle cell activity. In addition, it is very safe because of its low skin toxicity and excellent skin trouble.

The root of Mok-hyang, a perennial herb belonging to the Asteraceae, was dried. It is said to be effective in lowering blood pressure and anti-inflammatory.

Lycium chinense is the dried fruit of a wolfberry tree or other related plant belonging to the branch family. In oriental medicine, it has the effect of self-retaining nomenclature and lubricity. It is said to have the effects of immune regulation, hematopoietic function and anti-aging.

Prunella vulgaris var.lilacina is a dried perennial herbaceous nectar which is a herbaceous herb in oriental medicine. It is said to be effective in lowering blood pressure, anti-inflammatory, immunosuppression and antibacterial effects.

Kochia scoparia is a fruit of Dapsari, a year-old herbaceous plant belonging to the family of Ming Dynasty. It is said.

The present invention uses ultrasonic extraction instead of hot water extraction, room temperature extraction, warming extraction, etc., which are commonly used to prepare herbal extracts, and then, when filtering and removing impurities through a polymer ultrafiltration membrane, inhibiting hair loss and promoting hair growth. It provides a manufacturing method based on the surprising result that is greatly improved.

The extraction solvent used in the ultrasonic extraction of the present invention is purified water, methanol, ethanol, propanol, butanol, glycerol, propylene glycol, 1,3-butylene glycol, methyl acetate, ethyl acetate, nucleic acid, chloroform, benzene and petroleum ether. One or two or more kinds may be selected and used from the group consisting of, and the ultrasonic extractor is extracted for about 1 to 12 hours.

In addition, the herbal extract extracted using the ultrasonic wave as described above is a cartridge type pretreatment filter having a size of 1 to 50 micrometers to remove coarse particles such as wood, particulate matter, or insoluble suspended solids present in the extract. After passing through, the ultrafiltration membrane composed of polymers with a molecular weight of 5,000 to 300,000 Daltons is used to pass through most of the low-molecular substances and ions, which are present independently of ash, and to extract only active ingredients such as flavonoids and polyphenols in the permeate. The material of the polymer filtration membrane is preferably Regenerated Cellulose, Polyacrylonitrile (PAN), Polysulfone, Polyisosulfone, Polyvinylidene fluoride, etc. Do.

Herbal medicine extract obtained as described above preferably contains 0.0001 to 100% by weight.

In addition, to the composition of the present invention, in order to impart additional functions suitable for use by alopecia patients, auxiliary ingredients such as anti-dandruff, keratin softeners, coolants, and moisturizers may be added, and in this case, a better effect is obtained.

Hair growth promoter composition of the present invention can be prepared in any formulation that can be applied to the scalp, such as liquid, cream, paste or solid phase, shampoo, hair conditioner, hair lotion, And aerosol types of these formulations.

On the other hand, the skin anti-aging cosmetic composition containing the herbal medicine extract of the present invention is a common ingredient, such as oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives that are formulated in general skin cosmetics It is possible to apply and mix as many flavors as necessary.

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are merely provided to more easily understand the present invention, and the present invention is not limited to the following examples.

Claims (6)

Ultrasonic extraction of herbal medicines using an ultrafiltration membrane with molecular weight cut-off of 10,000 to 200,000 Daltons, which includes extracts filtered through permeation of independent low molecular weight substances, ions, and ashes as active ingredients. Hair loss prevention and hair growth promoting composition, characterized in that The method of claim 1, Hair loss prevention and hair growth promoting composition, characterized in that it further comprises a herbal medicine selected from the group consisting of Mok-hyang, wolfberry, hachicho and branch The method of claim 1, The composition of each dried herbal medicine is characterized in that it is mixed in the ratio of 0.01 ~ 10% by weight, 0.01 ~ 10% by weight, Goji vinegar 0.01 ~ 10% by weight, branch branch 0.01 ~ 10% by weight based on the solid content, respectively Hair Loss Prevention and Hair Growth Promoting Composition 3. The composition according to claim 1 or 2, wherein the extraction solvent of the hair loss preventing and hair growth promoting composition is one or two or more mixed solvents of a lower alcohol and a hydrocarbon-based solvent. The hair loss of claim 1, wherein the composition further comprises at least one additive selected from the group consisting of oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives and fragrances. Anti- and Hair Growth Promoting Composition A transdermal topical formulation for application to the scalp, comprising the hair loss prevention and hair growth promoter composition of claim 1