KR20100120431A - An acaricidal composition against tetranychus urticae, comprising edible oil emulsion and method of application - Google Patents

Abstract

PURPOSE: A composition containing cooking oil emulsified liquid and method for preventing using the same are provided to effectively remove eggs of Tetranychus urticae. CONSTITUTION: A composition for preventing Tetranychus urticae contains cooking oil emulsified liquid as an active ingredient. The cooking oil emulsified liquid is manufactured by stirring mixture of cooking and detergent for 20-30 minutes. The cooking oil is soybean oil, corn oil, sunflower seed oil, canola oil, olive oil and grape seed oil. The detergent is contained in 20-40 weight%. The detergent further contains solubilizing agent, additive and remaining amount of water.

Description

Composition for control of spotted mite containing an edible oil emulsion and a method for controlling the same {An acaricidal composition against Tetranychus urticae, comprising edible oil emulsion and method of application}

The present invention relates to a novel natural insecticide having excellent fertilizing effect, scattering inhibitory effect and spawning effect on spotted mite, and more particularly, to a composition for controlling spotted mite containing an edible oil emulsion as an active ingredient and a method for controlling the same will be.

Spotted mite ( Tetranychus urticae ) is an omnivorous pest that lives and harms all crops such as fruit trees, corn, and soybeans, and vegetables, flowers, weeds, common crops, and flowers. It occurs in many parts of the world, including Korea, Japan, the United States, and Europe, and the damage from facility cultivation crops is increasing due to its wide host range. It mainly lives on the back side of the leaves and sucks the juice. The damage leaves have yellow or white spots, and if the damage is severe, the whole leaves become yellowish brown and fall prematurely. Spotted mites are known to occur throughout the year in houses and over 8 to 10 generations a year. As the breeding speed increases, the density of occurrence increases rapidly, and the damage is rapidly advanced.

Therefore, control agents for controlling such mites have been developed and used. Examples of acaricides used for controlling mites include kelofen, tedion: tetradifon, chlorofenson, bromopropylate, There are dozens of species, such as moresene (chinomethionat), ferropal (azocyclotin), tasta bifenthrin granule smoker, Chias D-DV smoker (powering), phenprogranule smoker (Danitol). Spotted mite has a high density in the lower lobe close to the branch, so care should be taken to spray enough liquid on the lower part of the plant.

On the other hand, the conventional control means for the mite is a lot of dependence on the drug, due to the high cost and side effects of the drug is a situation that places a lot of burden on the farm. In addition, the productivity of agriculture has been greatly improved by the use of organic synthetic pesticides, but due to its excessive use, the current situation is that the pests and resistance of secondary pests such as spot mite have been greatly developed and are facing difficulties in controlling at the same time.

Particularly, in the control of mites, the developmental speed of the drug resistance of the mites is one of the great difficulties. The malignant resistance of the mites varies according to the types of acaricides, and even in the case of spotted mites, several organophosphorus pesticides such as parathion and organic A silkworm culling test with chlorine-based dipofol revealed significant differences in the resistance development of acaricides. On the other hand, acaricide-resistant mites are often pointed out as a big problem in carrying out chemical control because they often exhibit cross-resistance to other drugs not involved in culling, and they are excessive to control chemically resistant spotted mites. Pesticides are used, which causes problems such as destruction of agricultural ecosystems, environmental pollution, human toxicity, and the addition of drug-resistant pests.

Therefore, as a part of the solution to solve this problem, it is urgent to develop an environmentally friendly natural insecticide as well as an excellent insecticide effect, and in particular, the vegetable insecticide is attracting attention as one of the alternative pesticides as a biopesticide.

Therefore, the inventors of the present invention, while continuing to study the natural insecticide for effectively controlling the spotted mite to solve the problems of the prior art, the cooking oil emulsion mixed with the cooking oil and detergent shows excellent fertilizing effect and scattering inhibitory effect on the spotted mite The present invention has been completed by discovering that.

Therefore, it is an object of the present invention to provide a composition for controlling spotted mite containing an edible oil emulsion as an active ingredient.

In addition, another object of the present invention is to provide a spotted mite control method comprising the step of treating a spotted mite control composition containing an oil emulsion as an active ingredient to the habitat of the spotted mite or spotted mite.

In order to achieve the object of the present invention as described above, the present invention provides a composition for controlling the spotted mite containing the cooking oil emulsion prepared by mixing cooking oil and detergent as an active ingredient.

In one embodiment of the present invention, the cooking oil emulsion may be prepared by stirring the mixture of cooking oil and detergent for 20 to 30 minutes.

In one embodiment of the present invention, the mixing ratio of the cooking oil and detergent may be 1: 1 to 3: 1.

In one embodiment of the present invention, the cooking oil may be any one selected from soybean oil (soybean oil), corn oil, sunflower oil, canola oil, olive oil and grape seed oil.

In one embodiment of the present invention, the detergent may be one containing 20 to 40% by weight of the surfactant.

In one embodiment of the present invention, the surfactant is a linear alkylbenzene anionic surfactant, higher alcohol anionic surfactant, alpha olefin anion surfactant, sulfonate anion surfactant, sulfate anion surfactant, higher alcohol It may be a mixture of two or more selected from the group consisting of a nonionic surfactant, a fatty acid nonionic surfactant, an amine oxide cationic surfactant.

In one embodiment of the present invention, the detergent may further include a solubilizer, an additive and a residual amount of water.

In addition, the present invention provides a spotted mite control method comprising the step of treating the spotted mite control composition comprising the edible oil emulsion as an active ingredient in the habitat of the spotted mite or spotted mite.

In one embodiment of the present invention, the cooking oil emulsion may be treated by diluting 500 to 2,000 times with water.

Spotted mite control composition comprising the edible oil emulsion according to the present invention has excellent killing effect and spawning inhibitory effect on spotted mite adult, excellent killing effect on spotted mite eggs, very low spotted mite control rate at low concentration It is highly effective. In addition, the composition for controlling the spotted mite containing the cooking oil emulsion according to the present invention can be easily prepared by anyone, very low production cost, can be used as a natural insecticide, there is no toxicity to people or animals and plants and harmful to the environment There is no environmentally friendly effect.

The present invention relates to a novel natural insecticide having excellent fertilizing effect, scattering inhibitory effect and spawning effect on spotted mite, and more particularly, to provide a composition for controlling spotted mite containing an edible oil emulsion as an active ingredient and a method for controlling the same. It has its features.

In the present invention, it was first identified that the cooking oil emulsion prepared by mixing the cooking oil and the detergent at a predetermined ratio has excellent killing effect, scattering suppression effect and scattering effect on the spotted mite.

The cooking oil emulsion contained in the composition for spot mite control of this invention can be manufactured by mixing cooking oil and a detergent in a fixed ratio. At this time, the cooking oil emulsion is preferably mixing the cooking oil and kitchen detergent in a ratio of 1: 1 to 3: 1.

In one embodiment of the present invention was added to the cooking oil 20ml 10ml dish detergent and mixed for 20 to 30 minutes using a stirrer to prepare a cooking oil emulsion. In this case, when the foam is formed by the mixing detergent and the magnetic bar is no longer returned to the magnetic bar, the cooking oil emulsion according to the present invention is completed.

The cooking oil used to prepare the cooking oil emulsion of the present invention may be selected from any one of soybean oil (soybean oil), corn oil, sunflower oil, canola oil, olive oil and grapeseed oil, but is not limited to the cooking oil sold on the market. All ramen can be used.

In addition, the cooking oil can be extracted and purified from plants such as soybeans, corn, sunflowers, rapeseed, olives, grapes using conventional extraction and purification methods in the art. The type of extraction and purification methods in the art, but is not limited thereto, one method selected from steam distillation method, hot water extraction method, solvent extraction method, compression method, carbon dioxide gas extraction method, reflux cooling extraction method, cold leaching method, elution method It can be done with

On the other hand, general detergents are cleaning agents containing surfactant as a main component, and almost all such as textiles, pharmaceuticals, cosmetics, food, pesticides, civil engineering and construction, mining, paper and pulp, leather, dyes and pigments, paints and inks. It is used in the field.

The detergent used in the present invention may use a detergent containing a surfactant, and in particular, may use a kitchen detergent containing a surfactant.

In addition, in the case of kitchen detergent, the detergent may include a surfactant, a solubilizer and additives.

The surfactant may be used by mixing one or more surfactants selected from the group consisting of anionic surfactants, cationic surfactants and nonionic surfactants.

The anionic surfactant is preferably used at least one selected from the group consisting of linear alkylbenzene, higher alcohol, alpha olefin, sulfonate, sulfate system, for example, straight alkylbenzene sulfonate, alkyl sulfate Ester salt, alkyl ester sulfate ester salt, alpha olefin sulfonate, linear alkyl benzene sulfonate, paraffin sulfonate, fatty alcohol sulfate, fatty alcohol ethoxy sulfate, alkylbenzene sulfonate, olefin alkyl sulfonate, alkyl ether sulfate, alkyl sulfate , And secondary alkane sulfonates and the like are used, but are not limited thereto.

As the nonionic surfactant, it is preferable to use one or more selected from the group consisting of higher alcohols and fatty acids. For example, polyoxyalkylene alkyl ethers, polyoxyalkylene alkyl phenol ethers, polyoxyalkylene aryls. Phenol ethers, polyoxyalkylene fatty acid esters, polyoxyalkylene sorbitan fatty acid esters, monoglycerine fatty acid esters, polyglycol fatty acid esters, sucrose fatty acid esters, sorbitan fatty acid esters, fatty acid alkanolamides, polyoxyalkylene alkyl amines, Alkyl alcohol amines, aryl alcohol amines, and the like are used, but are not limited thereto.

As the cationic surfactant, it is preferable to use an amine oxide-based surfactant, and examples thereof include alkyldimethylamine oxide, alkylamidopropyldimethylamine oxide, and the like, but are not limited thereto.

As the solubilizer, ethanol, methanol, propanol, isopropanol, butanol, isobutanol, propylene glycol, ethylene glycol, polyethylene glycol, glycerin, propylene glycol, alkanols having 1 to 4 carbon atoms, alkylaryl sulfonates, carboxylic acid sulfates or sulfo One or more types can be selected and used from the group which consists of a salt of a salt, a urea, a hydrocarboxylate, a carboxylate, and mixtures thereof.

In addition, the additive may be used by selecting one or more from the group consisting of salts, preservatives, thickeners, enzymes, flavorings, dyes, pigments, pearls, emulsifiers, pH adjusting agents, metal sealants, skin protectants, moisturizers, antibacterial agents. have. At this time, the components and contents may be appropriately selected and adjusted by those skilled in the art, and may be blended in an appropriate amount as necessary in a range that does not impair the effects of the present invention.

In preparing the edible oil emulsion of the present invention, it is preferable to use the detergent containing 20 to 40% by weight of the surfactant, and 0.1 to 15% by weight of the solubilizer, 0.1 to 5% by weight of the additive and the remaining amount of water It may include.

In one embodiment of the present invention, a higher alcohol-based and alpha olefin-based anionic surfactant, an amine oxide surfactant was used as a cationic surfactant, a kitchen detergent containing a total of 30% by weight of these surfactant was used. .

On the other hand, the composition for controlling the spotted mite of the present invention containing an oil-based emulsion has not only excellent killing effect on the spotted mite, but also has an effect of suppressing scattering of the spotted mite.

According to one embodiment of the present invention, when the edible mite adult is treated with the cooking oil emulsion of the present invention, it was found that the adult insect of the spotted mite kills about 80% or more, and the total scattering number is also markedly reduced (Example 2).

In addition, the composition for controlling the spotted mite of the present invention has a scattering effect on the eggs of the spotted mite.

According to one embodiment of the present invention, when the edible oil emulsion of the present invention was treated to spotted mite eggs, it exhibited about 64% salinity (see Example 4).

In addition, the composition for controlling the spot mite according to the present invention is characterized in that even if stored for a long time at room temperature, the cooking oil emulsion, which is the main component of the composition, does not denature and maintains the fertilizing effect continuously.

According to one embodiment of the present invention, after treating the edible oil emulsion of the present invention for 1 week after storage at room temperature for 1 week, the insecticidal rate of about 78% appeared, through which the cooking oil emulsion is not denatured It was confirmed that the excellent acaricide effect was maintained (see Example 3).

Furthermore, the present invention provides a method for stepwise treatment of a spotted mite control composition comprising an edible oil emulsion as an active ingredient in a habitat of spotted mite or spotted mite.

When the composition for controlling the spotted mite according to the present invention is treated in the habitat of the spotted mite or spotted mite, it can be used by diluting the cooking oil emulsion with water 500 to 2,000 times.

In addition, the control method according to the present invention can be treated by spraying, dipping, coating, fumigation, spraying or injecting the spotted mite control composition of the present invention directly into spotted mite adult or eggs. In addition, it can be treated wherever spotted mite inhabits, and can be processed by spraying, dipping, applying, fumigation, spraying or injecting directly into the habitat of spotted mite. At this time, the applied dose of the composition of the present invention can be used as a dose to apply a conventional insecticide.

Spotted mite control composition of the present invention can be used in addition to other insecticides, nematicides, mites, fungicides, herbicides, plant growth regulators and the like.

In addition, the composition for controlling mucosa of the present invention can be prepared and applied in various forms. It can be prepared in the form of a liquid, emulsion, coating or granules by dissolving or dispersing in one or more solvents for application and dipping treatment, and extenders such as liquid solvents, using surfactants commonly used for fumigation treatments, It is preferably used in combination with a pressurized liquefied gas or a solid carrier in the form of a smoker, a fumigant, a coating agent, a granule, or a solid agent.

To prepare such various forms of preparations, solvents and additives commonly used in the art can be used in the compositions according to the invention.

In addition, the composition of the present invention may be prepared in solid form further comprising an excipient according to the method of use. The excipient uses a suitable material according to the use and application method of the composition, all materials included in conventional pesticides can be used.

In addition, the composition of the present invention may further include an aromatic component (fragrance) in order to remove the unpleasant odor and give a fresh aroma when using the composition in a residential space. The fragrance ingredient may be one selected from natural or chemically synthesized fragrances such as fruit and flower fragrances or a mixture of two or more thereof.

In addition, the composition of the present invention may further include a corrosion inhibitor selected from the group consisting of sodium nitrite, sodium benzoate and mixtures thereof, in order to inhibit corrosion of the metal container containing the composition.

Hereinafter, the present invention will be described in detail with reference to Examples and drawings. However, these examples are intended to illustrate the present invention in more detail, and the scope of the present invention is not limited to these examples.

< Example  1>

Cooking oil according to the present invention Of emulsion  Produce

The present inventors prepared a cooking oil emulsion by mixing 10ml of dish detergent in 20ml of cooking oil and mixing for 20 to 30 minutes using a stirrer to prepare an cooking oil emulsion having a control effect against spot mite. At this time, when the foam is formed by the detergent and the magnetic bar is no longer mixed so that the magnetic bar can not be used, the mixed solution was used in subsequent experiments. In addition, the detergent was used that contains a total of 30% of the higher alcohol-based anionic surfactant, alpha-olefin ionic anionic surfactant and amine oxide cationic surfactant.

< Example  2>

cooking oil Of emulsion  Spotted Acaricide effect

It was examined whether the cooking oil emulsion of the present invention prepared in Example 1 had an effect of fertilizing the adult of the spotted mite. To this end, first, a wet tissue was placed on a petri dish, a round kidney bean leaf slice (3 cm in diameter) was placed on the wet tissue, and 20 adult females were inoculated. At this time, the cooking oil emulsion was used by diluting 1000 times and 2000 times with water, respectively, and the control was diluted 1000 times and 2000 times with water and treated only with water and not treated anything. The kidney beans inoculated with the spotted M. nymphs were immersed in each substance (edible oil emulsion, detergent, and water) for 5 seconds, and after 24 hours, live insects, insects, and spawning water were checked, and after 48 hours Checked. This experiment was performed twice, three times in total, and the experimental results are shown in Table 1 below. Statistical analysis was performed using the Tukey HSD test method.

Experimental results on the fertilizing effect of cooking oil emulsion process diluent Insecticidal rate (after 24 hours) Insecticidal rate (after 48 hours) Total scattering cooking oil 1000 times 77.6 ± 16.9a 82.1 ± 10.4a 16.0 ± 15.1b 2000 times 75.1 ± 8.1a 80.6 ± 12.3a 19.7 ± 14.7b Detergent 1000 times 57.8 ± 14.6a 59.5 ± 17.4a 42.0 ± 23.6b 2000 times 61.4 ± 23.0a 75.2 ± 21.0a 16.3 ± 12.1b Distilled water - 7.2 ± 2.9b 7.2 ± 2.9b 210.1 ± 19.9a control - 0.0 ± 0.0b 0.0 ± 0.0b 211.0 ± 30.8b
Statistical analysis result df = 5,12;
F = 19.1;
P = <. 0001 df = 5,12;
F = 19.1;
P = <. 0001 df = 5,12;
F = 25.02;
P = <. 0001

As a result, as shown in Table 1 above, when the edible oil emulsion of the present invention was treated by diluting 1000 times with respect to spotted mites, the insecticide rate was 77.6% after 24 hours of treatment, and 82.1% after 48 hours. Pesticide rate was shown. Even when treated at a 2000-fold dilution, the insecticidal effect was about 75% or more. In addition, in the irradiation of total scattered water, the total scattered water was found to be remarkably reduced as compared to the case of treating distilled water or nothing.

<Example 3>

Room temperature cooking oil Of emulsion  Spotted mites Acaricide effect

In order to determine the degree of acaricide effect compared to the cooking oil emulsion prepared immediately after cooking oil emulsion stored at room temperature was tested as follows. The cooking oil emulsion prepared in Example 1 was used for the experiment after storage at room temperature for 1 week, and proceeded in the same manner as in Example 2.

Experimental Results on the Fertilizing Effect of Edible Oil Emulsions at Room Temperature process diluent Insecticidal rate (after 24 hours) Insecticidal rate (after 48 hours) Total scattering Cooking Oil Emulsion
1000 times 36.2 ± 21.8 b 48.4 ± 18.8 b 74.3 ± 39.5 c 2000 times 69.1 ± 2.1 a 78.4 ± 5.0 a 26.0 ± 19.3 c Distilled water - 9.1 ± 8.4b c 15.1 ± 7.6 c 161.3 ± 10.69 a control - 0.0 ± 0.0 c 1.7 ± 2.9 c 230.7 ± 18.58 b
Statistical analysis result
df = 3, 8
F = 21.08
P = 0.0004 df = 3, 8
F = 32.02
P = <. 0001 df = 3, 8
F = 41.52
P = <. 0001

As a result, as shown in Table 2, even after one week of storage at room temperature, the 2000-fold dilution of the cooking oil emulsion exhibited a pesticide rate of about 78%. Could know. In addition, it was confirmed that the scattering avoidance effect is also maintained.

< Example  4>

cooking oil Of emulsion  Spotted mite Scattering effect

It was investigated whether the cooking oil emulsion according to the present invention had a scattering effect on eggs of spotted mites. For this, first, a wet tissue was placed on a petri dish, a round kidney bean leaf slice (3 cm in diameter) was placed on the wet tissue, and 10 adult females were inoculated. . At this time, the cooking oil emulsion was used by diluting 1000 times and 2000 times with water, respectively, and the control was diluted 1000 times and 2000 times with water and treated only with water and not treated anything. The kidney beans with eggs scattered by the adult mite were spotted in the respective materials (edible oil emulsion, detergent and water) for 5 seconds. Thereafter, hatching rates after 24 hours, after 3 days, and after 4 days were checked respectively. This experiment was repeated three times in total, and the experimental results are shown in Table 3 below. Statistical analysis was performed using the Tukey HSD test method.

Experimental results on the scattering effect of cooking oil emulsion process diluent Spawn rate (after 24 hours) Cooking Oil Emulsion
1000 times 63.8 ± 14.8 a 2000 times 19.3 ± 8.9 b Detergent
1000 times 5.8 ± 5.7 b 2000 times 22.8 ± 21.8 b Distilled water - 0.6 ± 1.0 b control - 0.8 ± 1.5 b df = 5, 12
F = 12.74
P = 0.0002

As a result, when the edible oil emulsion of the present invention was treated with spotted mite eggs, the egg oil of the present invention exhibited about 64% salinity in a 1000-fold dilution of the edible oil emulsion. It could be confirmed.

Therefore, the present inventors have demonstrated that the cooking oil emulsion according to the present invention has excellent killing effect and scattering inhibitory effect on the spotted mite, and excellent scattering effect on the eggs of the spotted mite.

So far I looked at the center of the preferred embodiment for the present invention. Those skilled in the art will appreciate that the present invention can be implemented in a modified form without departing from the essential features of the present invention. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope will be construed as being included in the present invention.

Claims (8)

A composition for controlling spotted mite containing an cooking oil emulsion prepared by mixing cooking oil and a detergent as an active ingredient. The method of claim 1, The cooking oil emulsion is spotted mite control composition, characterized in that prepared by stirring the mixture of cooking oil and detergent for 20 to 30 minutes. The method of claim 1, The mixing ratio of the cooking oil and detergent is spotted mite control composition, characterized in that 1: 1 to 3: 1. The method of claim 1, The cooking oil is a soybean oil (soybean oil), corn oil, sunflower oil, canola oil, olive oil and grape seed oil any one selected from the composition for the control of spotted mite. The method of claim 1, The detergent is spotted mite control composition, characterized in that containing 20 to 40% by weight surfactant. The method of claim 5, The surfactant may be a linear alkylbenzene anionic surfactant, a higher alcohol anionic surfactant, an alpha olefin anionic surfactant, a sulfonate anionic surfactant, a sulfate anionic surfactant, a higher alcohol nonionic surfactant, or a fatty acid ratio. A composition for controlling mucus mite characterized by using at least one selected from the group consisting of an ionic surfactant and an amine oxide cationic surfactant. The method of claim 5, The detergent is a solubilizing agent, an additive and a composition for controlling mucus mite, characterized in that it further comprises a residual amount of water. The method of claim 1, The composition is spotted mite control composition characterized in that it has an astringent effect, scattering inhibitory effect and scattering effect against the spotted mite.