KR20100117262A - Composite of toothpaste antibiosis - Google Patents

Abstract

PURPOSE: An antibacterial tooth paste composition for preventing dental caries and periodontal diseases is provided to ensure strong sterilizing effect to streptococcus mutans. CONSTITUTION: An antibacterial tooth paste composition contains 0.6-3.0 weight% of mixture solution containing Sophorae Radix extract and Forsythiae Fructus extract based on total weight of tooth paste base. The tooth paste base contains 15-50 weight% of abrasive of calcium carbonate, calcium pyrophosphate, calcium metaphosphate, silica, aluminum silicate, and aluminum hydroxide; 0.5-4.0 weight% of tackifier of carboxy methyl cellulose, hydroxy methyl cellulose, carrageenan, Arabia gum, and vinyl alcohol; 5.0-20 weight% of wetting agent of polyethylene glycol, sorbitol, and glycerin polyvinyl alcohol; and 10-50 weight% of perfume, saccharin, antiseptic, and purified water.

Description

Antimicrobial toothpaste composition {Composite of toothpaste antibiosis}

The present invention relates to a dentifrice composition incorporating a water-soluble extract obtained from Rhododendron, Ginseng and Yeongyo. More specifically, the present invention has a strong bactericidal activity against the causative agents of caries and periodontal disease, among the mixed water-soluble extracts of Mansi, Ginseng and Fructus, which are known to be effective in the prevention of oral diseases. The present invention relates to an antimicrobial toothpaste composition for preventing tooth decay and periodontal disease caused by plaque formation by efficiently combining antimicrobial components that inhibit secretory activity of transferase (Glucosyltransferase: GTase).

In general, tooth decay is called dental caries, and it is one of the oral diseases caused by the interaction of bacteria, food, and saliva in the plaque, and the incidence rate is increasing in Korea as well as in developed countries.

The main cause of tooth decay is Streptococcus mutans (S. mutans), which is known to act as the main cause of bacteria and adheres to the oral tooth surface and generates acid by fermentation of sugars to destroy the tooth surface.

In other words, Streptococcus mutans secretes glucosyltransferase (GTase) into the cell surface layer to form insoluble polysaccharides (Glucan) from sucrose in food, and these insoluble polysaccharides form bacterial membranes (or plaque) on oral microorganisms and tooth surfaces. ), And the bacteria gathered here break down sugars to produce acid, which demineralizes the enamel surface of the tooth, causing the caries to progress. If plaque further progresses to tartar, it will cause periodontal disease of gingivitis, gingivitis, and alveolar pylori.

As a method for preventing such oral diseases, a preservative disinfectant such as parahydroxybenzoic acid ester, phenolic chloride, benzoic acid and its salts, fluorine compound, sodium dihydroacetate, strontium chloride, etc. is used as a main component of toothpaste. , Antiseptic fungicides of the above ingredients cause tooth decay, gingivitis, gingivitis, alveolar pylori, and acting to sterilize or inhibit the metabolism of microorganisms in the oral cavity causing bad breath.

However, antiseptic fungicides generally used are inactivated by surfactants or abrasives contained in toothpaste compositions, and thus the activity of the preservatives is often lowered. On the other hand, antiseptic fungicides are used to prevent the reduction or inactivation of antiseptic power. Due to excessive addition of stimulation of the oral mucosa may cause swelling, blisters or erythema, or may cause central nervous system disorders, and in the case of fluorine compounds there were disadvantages that cause spots on the teeth.

In order to minimize the side effects caused by using such antiseptic fungicides, there are known techniques for combining various plant extracts with toothpaste compositions.

For example, Japanese Patent No. 1986-197510 uses an extract extracted from the leaves of Camellia plants such as Tea tree for periodontal disease, but in this case the toothpaste changes color or aroma during long-term storage. There is a disadvantage that the antimicrobial effect is reduced or the merchandise is inferior in use.

The causes of discoloration (browning reaction) in natural plant extracts can be divided into two main reasons. The first is the Maillard reaction, a non-enzymatic browning reaction, which causes discoloration of toothpaste by forming a yellowish brown polymer by reacting the amino group of an amino acid compound with a compound having an aldehyde or a catone group among natural products or medicinal agents used in toothpaste. Long-term use of toothpaste is known to cause discoloration. The second is browning by polyphenol oxidase, an enzymatic browning reaction, and polyphenols known as pragze or odor inhibitors in natural plant extracts are exposed to the air. It is known that discoloration occurs as conversion to phosphorus melamine occurs.

In addition, US Pat. No. 5,526,684 uses tea tree oil extracted from tea tree as a flavoring agent to enhance the therapeutic effect of pragse or gingivitis, but in this case, the amount of tea tree oil is used at a content level that achieves an appropriate flavor. There is a limit that the usage can not be limited.

In addition, Japanese Patent No. 1984-134729 (Licorice, Morus bark) containing plant extract, Japanese Patent No. 1988-253019 (Chinese Herb), Japanese Patent No. 1983-39614 (Salvia, Rosemary), Japanese Patent Laid-Open No. 1-190624 (Green tea) , Oolong tea, black tea), Japanese Patent No. 1987-48616 (mint, rosemary), Japanese Patent No. 1987-58331 (Senega, hazelnut) are known, but they also have a lower antimicrobial effect against caries and periodontal pathogens. In addition, there was a problem that the activity inhibitory effect of the extracellular enzyme glucosyltransferase (GTase) of streptococcus mutans plays an important role in the formation of plaque.

Accordingly, an object of the present invention is to provide a strong bactericidal action against streptococcus mutans known as cavities and periodontal disease, while minimizing side effects on oral mucosa and human body caused by overdosage of fungicides. The present invention provides an antimicrobial toothpaste composition that prevents tooth decay and periodontal disease. The antimicrobial toothpaste is formulated with a common toothpaste base by mixing a mixed aqueous extract of Manzacho, Ginseng, and Fructus, harmless to the human body, which is known to be effective in the prevention of oral diseases. Is achieved.

Another object of the present invention is to provide an antimicrobial toothpaste composition excellent in antimicrobial effect against caries and periodontal causative bacteria and inhibitory effect of extracellular GTase of S. mutans with small amount of plant extract.

Other objects of the present invention will become more apparent from the following detailed description.

According to the antimicrobial toothpaste composition according to the present invention for achieving the above object, 15 to 50% by weight of abrasives such as calcium carbonate, calcium pyrophosphate, calcium metaphosphate, silicas, aluminum silicate, aluminum hydroxide, carboxymethyl cellulose, 0.5 to 4.0% by weight of thickeners such as hydroxymethyl cellulose, carrageenan, gum arabic, polyvinyl alcohol, polyethylene glycols, sorbitol, 5.0 to 20% by weight of humectants such as glycerin polyvinyl alcohol, sodium lauryl sulfate, sodium lauryl sarco Ordinary toothpaste base prepared by mixing 1.0 to 2.0% by weight of foaming agents such as sinate, monoglyceride sulfate, sucrose fatty acid ester, and other components such as flavoring agents, saccharin, stevioside, and sweeteners and preservatives with 10 to 50% by weight of purified water. In the same manner, the red ginseng extract, the poncho extract, and the panacea extract were mixed at the same weight percent, and the mixed water-soluble weight It is characterized by containing the extract liquid 0.6-3.0 weight%.

Toothpaste prepared by the addition of the plant extract of the present invention is the addition of various active ingredients commonly used in toothpaste such as sodium fluoride, sodium monofluorophosphate, salt, allantoin, aminocaroroic acid, vitamins, zinc chloride, cetylpyrididium chloride, etc. It is also possible.

According to the antimicrobial toothpaste composition of the present invention, the mixed water-soluble extract obtained from red ginseng, poncho and mandala has a strong bactericidal action against S. mutans, thus preventing caries and periodontal disease, and inhibiting the secretion activity of GTase. Tooth decay and periodontal disease caused by the formation is effective to prevent.

Hereinafter, a preferred embodiment of the antimicrobial toothpaste composition according to the present invention will be described in detail.

The duct used in the present invention refers to the fruit of the forsythia in one shot, the deciduous, deciduous shrub belonging to the ash tree and the scientific name is Posyria correa (Forsythia Koreana), the forsythia which opens the fruit (compassion) is a three-leaved forsythia, In the fall, pick your fruit and use it to dry in the sun. Among the components of ducts, skins include oleanolic acid, tannin, phylligenin, and monoterpan glycosides (protiated, procedural monoesters). The fruit contains prostol, a sterol compound, sapinin, a flavonol glycoside, and matairesinoside. Extracted aqueous solution has strong antibacterial activity against typhoid bacteria, paratyphoid bacteria, Escherichia coli, Pseudomonas aeruginosa, and staphylococcus aureus, streptococcus, pneumonia, pertussis bacterium, as well as antibacterial anti-inflammatory agents such as inflammation, drainage, venom, skin disease, wound, skin It is said to treat rashes, eczema, toxins and acne.

In addition, recently, 0-157 bacteria and SARS causative agents are known to have a high antimicrobial activity against the corona virus, in the present invention when applied as a toothpaste formulation through the embodiment, antibacterial against caries and periodontal causative bacteria It was confirmed that the effect and the inhibitory effect of GTase.

In addition, the ginseng used in the present invention is a dried plant that grows in a sunny meadow called sperm, similar to the efficacy of ginseng and bitter taste. The extract of the ginseng has the function of insecticidal, among which the 5-alpha reductase (5-alfa reductase) has been reported to have the effect of inhibiting gastric odor bad breath, dry mouth symptoms and gum bleeding.

In addition, the panacea used in the present invention is called cheonsangcho, cactus perennial herb, windy leaves, Seoknamyeop, etc., as the evergreen broad-leaved shrub belonging to the Rhododendron family, the scientific name is Rhododendron brachycarpum (Andromedo on the leaves of this plant) It contains ingredients such as dotoxin, abicurin, hypereroside, and oleanolic acid, which are excellent for tonic, gangjeong, coercive, antipyretic, anti-inflammatory and analgesic.In particular, andromedotoxin has antibacterial activity. It is known to have excellent insecticidal effects on bacteria such as microorganisms in the oral cavity.

Representative examples of extract preparation of the present invention are as follows.

Extract Preparation Example

400 g of 70% water was added to 50 g of pulverized dried medicinal herb, which was reacted at room temperature for 60 hours for 8 hours, and then concentrated under reduced pressure with a rotary evaporator to obtain 20 g of a concentrate. The dried ginseng of other dry medicinal herbs and panacea were extracted in the same manner as above to obtain 20 g of concentrate.

Example 1

[Antibacterial Activity Measurement in Toothpaste Base]

1) Preparation of the Composition

Toothpaste of the following formulation was prepared according to the conventional method of formulating the herbal extract prepared according to the method of extract preparation example.

2) Experiment Method

10 g of each toothpaste prepared according to the above formulation was added to 10 ml of Brain Hert Infusion Broth (BHI) containing 2% by weight of sugar and completely dispersed, and then, it was converted into Brain Hert Infusion Broth (BHI) to a toothpaste concentration of 0.06 to 0.18% by weight. After dilution, inoculated caries (S. mutans: ATCC 25175) and periodontal disease-causing bacteria (Actinomyces vicosus: ATCC 15987) were inoculated and incubated for 24 hours in a 37 ℃ incubator. After incubation, the antibacterial activity of the two kinds of bacteria according to the toothpaste concentration and the herbal extract concentration was obtained as shown in Tables 1 and 2.

TABLE 1

Antimicrobial Activity against Toxicity of Caries (S. mutans: ATCC 25175) by Plant Extracts in Toothpaste Base

TABLE 2

Antimicrobial Effect of Periodontal Disease-causing Bacteria (Actinomyces viscosus: ATCC 1578) by Plant Extracts in Toothpaste Base

Sodium lauryl sulfate is formulated as a blowing agent in toothpaste, 1-2% by weight of the total content in the toothpaste base, which itself is known to have some degree of antibacterial activity. As shown in Tables 1 and 2, the mixed water-soluble extracts of Ginseng, Yeongyo, and Rhododendrons in the dentifrice composition showed much better antimicrobial activity than that of sodium lauryl sulfate alone.

[Example 2]

[Measurement of Glucosyltransferase (GTase) Inhibitory Effect in Toothpaste Base

1) Preparation of the Composition

The composition was prepared in the same manner as in Experimental Example 1, and used in the experiment.

2) Experiment Method

① GTase Separation and Purification

Inoculated Streptococcus mutans ATCC 25175 into 3 liters of Brain Hert Infusion Broth (BHI), incubated in a 37 ° C incubator for 18 hours, centrifuged (3000 rpm, 20 minutes) to remove the cells, and the supernatant was ammonium sulfate. After saturation of 60 to 70 wt%, the mixture was left at 4 ° C for 18 hours. The precipitate thus produced was centrifuged (3000 rpm, 20 minutes), dissolved in 200 ml of potassium phosphate buffer (0.05 M, pH 6.5), and dialyzed with the same buffer for 24 hours. The dialysis solution was again centrifuged to remove insoluble materials. The crude enzyme solution thus obtained was injected into a column filled with hydroxyapatite, and the same buffer solution having different ionic strengths was eluted step by step to purify GTase. Get

② Measurement of GTase Inhibition Activity

10 g of each toothpaste prepared according to the above formulation was distilled into 4 ml of 0.05M potassium phosphate buffer solution (pH 6.5) containing 50 to 100 µg of GTF prepared by the above method, 70 µg of sugar, and 0.02 wt% NaN ₃ . The solution was diluted to 20% by weight and then centrifuged (3000 rpm, 20 minutes) to obtain a supernatant. After diluting the solution to a toothpaste concentration of 0.67 and 1.5% by weight, it was added to a buffer solution and reacted with an anti-silver group for 18 hours at 37 ° C., and the amount of glucan produced was measured as absorbance (660 nm) in a spectrophotometer. The decrease in absorbance at this wavelength is proportional to the inhibition of glucan production, ie GTase inhibition. GTase inhibitory activity was determined by the following equation, and the absorbance of the control solution was the absorbance of the reaction solution except the plant extract.

GTase inhibitory activity (%) = (absorbance of 1 sample solution / absorbance of control solution × 100)

The result shown in Table 3 was obtained by the said method.

[Table 3]

GTase Inhibitory Effect of Plant Extracts on Toothpaste Base

Sodium lauryl sulfate in toothpaste has a slight GTase inhibitory effect, but it is not satisfactory to prevent tooth decay, so it is necessary to add another GTase inhibitor. As shown in Table 3, when toothpaste was formulated with plant extracts such as red ginseng, yeonkyo, and hyacinth on the prescription, it showed much better effect than that containing sodium lauryl sulfate. It is expected to have a caries prevention effect.

As shown in the results of Table 1-3, the mixed water-soluble extracts of Ginseng, Yeongyo, and Manzacho were mixed in the toothpaste prescription, and compared to the toothpaste without the mixed water-soluble extracts. It showed antibacterial and GTase inhibitory effects. Therefore, the mixed plant extracts according to the present invention can expect excellent caries and periodontal disease prevention effect.

Specific embodiments of the present invention are as follows.

Example 3

Example 4

Example 5

Claims (1)

An antimicrobial dentifrice composition comprising 0.6% by weight to 3.0% by weight of a mixed extract of a high ginseng extract, a poncho extract, and a panax herb extract at an equivalent rate, based on the total weight of the toothpaste base.