KR20100073705A - Composition for anti-diabetes - Google Patents

Abstract

PURPOSE: An anti-diabetic composition is provided to treat or prevent diabetes complications and to make beverages for preventing or treating diabetes with underground water from Jeju Island. CONSTITUTION: An anti-diabetic composition includes beneficial components or underground water filtered and gained by collecting underground water from Jeju Island. The water is evaporated completely. The anti-diabetic composition comprises a sweetener, a flavorant, physiologically active components, minerals, a preservative, an emulsifier, an acidulant, and a thickener. The physiologically active components are catechin, epicatechin, gallocatechin, epigallocatechin, retinol, ascorbic acid, tocopherol, calciferol, thiamine, or riboflavin.

Description

Antidiabetic composition {Composition for Anti-Diabetes}

The present invention relates to an antidiabetic composition.

Diabetes is a condition in which insulin, a glucose-regulating hormone secreted by the pancreatic β-cells, is insufficient or does not work properly, causing blood glucose to accumulate in the blood rather than being used as energy, causing hyperglycemia and detecting glucose in the urine.

Diabetes is usually divided into insulin dependent diabetes (type I diabetes) and insulin independent diabetes (type II diabetes). Insulin-dependent diabetes mellitus is caused by viral infections and is caused by the destruction of the beta cells of the pancreas, resulting in insulin secretion. It is also known as pediatric diabetes because it occurs mainly in young age groups of 10 to 20 years. It is given because it is difficult to maintain life. Insulin-independent diabetes is caused by the lack of insulin in the pancreatic beta cells, but the amount is reduced and its action is caused, mainly after 30s, it is also called adult diabetes. Although it is called insulin-independent diabetes, it does not mean that insulin therapy is not necessary for hyperglycemia.

If diabetes persists for a long time, glucose is not properly absorbed into the body, and various metabolic disorders occur due to various metabolic disorders due to an imbalance in nutritional supply.

Diabetes complications include nerve complications caused by damage to nerve fibers and nerve membranes, diabetic retinopathy (non-proliferative retinopathy, proliferative retinopathy, diabetic cataracts), renal failure, sexual dysfunction, skin diseases (allergic), hypertension, atherosclerosis, stroke ( Stroke, heart disease (myocardial infarction, angina pectoris, heart attack), and gangrene.

Biguanides, thiazolidinediones, sulfonylureas, benzoic acid derivatives and α-glucosidase inhibitors are used to treat diabetes. Diabetes treatment with drugs has many side effects, and the World Health Organization (WHO) strongly recommends the use of natural products with fewer side effects. (Grover JK, Vats. V., Rathi. SS, Journal of Ethnopharmacology, 73 , pp 461-470, 2000).

Natural products reported to be effective in treating diabetes include moxibustion extracts (Korean Patent No. 464815), Apanaic acid derivative compounds isolated from Jangung Beetle Mushrooms (Korean Patent No. 457690), and extracts of hawthorn trees (Korean Patent No. 441787). , Polysaccharides isolated from Korean Angelica gigas (International Publication No. 2001-60386), bile of ruminant animals (US Patent No. 6645355) and the like.

The present invention also discloses the antidiabetic effects of natural products.

It is an object of the present invention to provide an antidiabetic composition.

Other objects and specific aspects of the present invention will be presented below.

The present inventors induced diabetes by administering alloxan (alloxan) that destroys β cells of the pancreas to rats, and then freely ingested groundwater taken from 10 groundwater wells in Jeju Island with solid feed for 8 weeks. It was confirmed that the degradation. On the other hand, the control group, which was freely ingested with solid feed instead of groundwater, died all three days after diabetes induction.

These results show that the groundwater taken from 10 groundwater wells in Jeju Island can be effectively used for antidiabetic use.

In one aspect, the present invention can be understood as an antidiabetic beverage composition comprising groundwater obtained by harvesting and filtering in the groundwater wells identified in (1) to (10) below or a dehydrated form thereof as an active ingredient. .

(1) 5m above sea level, 60m above sea level, 2175-1 Sagye-ri, Andeok-myeon, Namjeju-gun, Jeju-do, Korea;

(2) 5m above sea level, 396 Ongpo-ri, Hallim-eup, Bukjeju-gun, Jeju-do, 70m drilling depth;

(3) 4m above sea level, 396 Ongpo-ri, Hallim-eup, Bukjeju-gun, Jeju-do, 70m excavation depth;

(4) 37m above sea level, 344 Sangwi-ri, Aewol-eup, Bukjeju-gun, Jeju-do, 90m deep;

(5) 295m above sea level, 640-1 Saekdal-dong, Seogwipo-si, Jeju-do, 301m;

(6) 416m above sea level, No. 52, Saekdal-dong, Seogwipo-si, Jeju-do, 400m deep;

(7) 275m above sea level, 2863 Wahul-ri, Jocheon-eup, Bukjeju-gun, Jeju-do, 315m with excavation depth;

(8) 6.0m above sea level, 2172-1 Sagye-ri, Andeok-myeon, Namjeju-gun, Jeju-do, 67m deep;

(9) 143m above sea level, 1257 Sangmyeong-ri, Hallim-eup, Bukjeju-gun, Jeju-do, 170m excavation depth; And

(10) 271m above sea level, 2422-9 Seohong-dong, Seogwipo-si, Jeju-do 296m

In the present specification, "altitude" means the height of the zone based on the sea level and "excavation depth" means the excavation depth based on the ground surface.

In addition, the term "diabetes" herein includes insulin-dependent diabetes mellitus and insulin-independent diabetes mellitus as described above, and furthermore diabetes mellitus, such as hyperthyroidism and adrenal cortex hyperactivity, which occurs when the pancreas is damaged by other diseases. It is meant to include diabetes due to growth hormone hyperplasia or catecholamine hyperplasia, and gestational diabetes.

In addition, the term "active ingredient" as used herein means a component that can exhibit the desired activity alone or in combination with a carrier that is not active itself.

In addition, in this specification, "dehydrated form of groundwater" means any obtained when the groundwater obtained in the groundwater well is filtered and then dewatered. When fully dehydrated, a powdered product will be obtained, which includes the powdered product. The result in the form of a powder would be the composition of the mineral components identified in Table 2 below.

In addition, the meaning of "anti-diabetic" in the present specification includes the prevention, amelioration, treatment of pathological symptoms, such as hyperglycemia caused by the lack of insulin or the lack of insulin, the detection of glucose in the urine, delayed onset of these pathological symptoms I mean.

The anti-diabetic beverage composition of the present invention may be groundwater itself obtained by collecting and filtering, or dehydrated groundwater (in this case, the concentration of mineral components contained in the groundwater will be increased).

In the case of the antidiabetic beverage composition of the present invention, in particular, a beverage composition containing the resultant in the form of a powder obtained after complete dehydration as an active ingredient, other additives commonly used in the preparation of the beverage composition may be included.

Additives that may be included in the antidiabetic beverage composition of the present invention include sweeteners, flavoring agents, bioactive ingredients, minerals and the like.

Sweeteners can be used in amounts such that the beverage gives a suitable sweetness, and can be natural or synthetic. Preferably, a natural sweetener is used. Examples of the natural sweetener include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose and maltose.

Flavoring agents can be used to enhance the taste or aroma, both natural and synthetic. It is the case of using a natural thing preferably. In addition to flavors, the use of natural ones can be combined with nutritional purposes. The natural flavor may be obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or may be obtained from green tea leaves, round leaves, jujube leaves, cinnamon, chrysanthemum leaves, jasmine and the like. In addition, ginseng (red ginseng), bamboo shoots, aloe vera, ginkgo and the like can also be used. Natural flavors can be liquid concentrates or solid extracts. In some cases, synthetic flavoring agents may be used, and synthetic flavoring agents may be used in the form of esters, alcohols, aldehydes, terpenes and the like.

As the physiologically active substance, catechins such as catechin, epicatechin, gallocatechin, epigallocatechin, vitamins such as retinol, ascorbic acid, tocopherol, calciferol, thiamine, riboflavin, and the like can be used.

As minerals, calcium, magnesium, chromium, cobalt, copper, fluoride, germanium, iodine, iron, lithium, magnesium, manganese, molybdenum, phosphorus, potassium, selenium, silicon, sodium, sulfur, vanadium, zinc and the like can be used. .

In addition, the beverage composition of the present invention may contain a preservative, an emulsifier, an acidulant, a thickener, and the like, in addition to the sweetener.

Such preservatives, emulsifiers and the like are preferably added and used in very small amounts as long as the use to which they are added can be achieved. By trace amounts it is meant numerically in the range from 0.0005% to about 0.5% by weight, based on the total weight of the beverage composition.

Examples of preservatives that can be used include sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, EDTA (ethylenediaminetetraacetic acid), and the like.

Emulsifiers that can be used include acacia gum, carboxymethylcellulose, xanthan gum, pectin and the like.

Examples of acidulants that may be used include lead acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid, and the like. Such acidulant may be added so that the beverage composition is at an appropriate acidity for the purpose of inhibiting the growth of microorganisms in addition to the purpose of enhancing taste.

Thickeners that can be used include suspending implements, sedimenters, gel formers, swelling agents and the like.

In another aspect, the present invention can be regarded as an antidiabetic pharmaceutical composition comprising a concentrated powder of groundwater obtained by collecting and filtering in the groundwater wells of (1) to (10).

The pharmaceutical composition of the present invention includes pharmaceutically acceptable carriers, excipients, and the like in addition to the concentrated powder of groundwater, oral formulations (tablets, suspensions, granules, emulsions, capsules, syrups, etc.), parenteral formulations (sterile strains). Aqueous or oily suspension) and the like.

As used herein, "pharmaceutically acceptable" means that the subject of application (prescription) does not have more toxicity (adequately low toxicity) to which the subject of application (prescription) is adaptable without inhibiting the activity of the active ingredient.

Examples of pharmaceutically acceptable carriers include lactose, glucose, sucrose, starch (such as corn starch, potato starch, etc.), cellulose, derivatives thereof (such as sodium carboxymethyl cellulose, ethylcellulose, etc.) malt, gelatin, talc, solids Lubricants (e.g. stearic acid, magnesium stearate, etc.), calcium sulfate, vegetable oils (e.g. peanut oil, cottonseed oil, sesame oil, olive oil, etc.), polyols (e.g. propylene glycol, glycerin, etc.), alginic acid, emulsifiers (e.g. TWEENS), wetting agents (e.g. Sodium lauryl sulfate), colorants, flavoring agents, tableting agents, stabilizers, antioxidants, preservatives, water, saline, phosphate buffer solutions and the like. Such a carrier may be used by selecting one or more appropriate ones according to the formulation of the pharmaceutical composition of the present invention.

Excipients may be selected and used according to the formulation of the pharmaceutical composition of the present invention, for example, when the pharmaceutical composition of the present invention is prepared with an aqueous suspending agent, suitable excipients are sodium carboxymethyl cellulose, methyl cellulose, hydropropylmethyl cellulose And suspending agents and dispersing agents such as sodium alginate and polyvinylpyrrolidone. Suitable excipients when prepared as injections include Ringer's solution, isotonic sodium chloride, and the like.

The pharmaceutical composition of the present invention may be administered orally or parenterally.

The daily dosage of the pharmaceutical composition of the present invention is usually 0.001 ~ 150 mg / kg body weight range, it can be administered once or divided into several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as the route of administration, the age, sex, weight of the patient, the severity of the patient and the like, the dosage may limit the scope of the present invention in any aspect. It should not be understood as.

In another aspect, the present invention relates to a beverage composition for treating or preventing diabetic complications comprising groundwater obtained by collecting and filtering in the groundwater wells of (1) to (10) or a dehydrated form thereof as an active ingredient. .

In another aspect, the present invention relates to a pharmaceutical composition for treating or preventing diabetic complications comprising groundwater obtained by collecting and filtering in the groundwater wells of (1) to (10) or a dehydrated form thereof as an active ingredient. .

Considering that it is generally necessary to lower hyperglycemia to normal levels for the treatment or prevention of diabetic complications (Kim, Sang-Sang, Chang-Soo Na: Effect of Phenollic Compounds Extracted from Pear on Streptozotocin-induced Hyperglycemic Mice, Korean Society of Food and Nutrition, 31 (6), 1107-1111, 2002), The composition for the treatment or prevention of diabetic complications of the present invention showing a hypoglycemic effect may be used alone or in combination with other diabetic complications or preventive agents for the treatment or prevention of diabetic complications. have.

In the present specification, the "diabetes complication" is meant to include all diseases secondary to the cause of diabetes, such as neurological complications, diabetic retinopathy, renal failure, sexual dysfunction, skin disorders, such as listed above , High blood pressure, atherosclerosis, stroke, heart disease, necrosis and the like.

In the beverage composition for treating or preventing the diabetic complications of the present invention or the pharmaceutical composition for treating or preventing the diabetic complications, the bar described in connection with the anti-diabetic beverage composition or the anti-diabetic pharmaceutical composition of the present invention is effective as it is.

On the other hand, when the compositions of the present invention as described above contains a concentrated powder as an active ingredient, such a concentrated powder may be included in any amount (effective amount) as long as it can exhibit the efficacy of anti-diabetes or improved efficacy of diabetic complications. An effective amount of phosphorus will be determined within the range of 0.001% to 10% by weight, based on the total weight of the composition. The term “effective amount” herein refers to an amount of an active ingredient that can lead to the prevention, improvement, treatment of diabetes or diabetic complications, or to delay the onset of such pathological symptoms. Such effective amounts can be determined experimentally within the range of ordinary skill in the art.

As described above, the present invention can provide an antidiabetic composition. Such compositions may be used as beverage compositions or pharmaceutical compositions.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

<Reference Example> Drinking water

There are about 6,000 groundwater wells in Jeju Island including saltwater and sewage, and among them, more than 1,400 minerals, including vanadium, are not sensitive to changes in the surrounding environment. The groundwater wells, which are considered to be in good condition and whose merchandise value is somewhat high, were selected and compressed into 10 locations. These 10 locations and the results of the water quality analysis are shown in <Table 1> and <Table 2>, respectively.

TABLE 1

Location of groundwater well

TABLE 2

Water quality analysis result by the well of collected groundwater

In Table 2, the pH was measured using a pH meter (Mettler Toledo Sevenmulti, S40), and the EC was measured using a Conductivity meter (Mettler Toledo Sevenmulti, S70). Ion chromatography (DIONEX 500) was used for the anionic components (F-, Cl-, NO ₃ -N, SO ₄ ^-2 ), and HCO ₃ ^- was used as the indicator bromocresol according to the Standard Methods (APHA-AWWA-WPCF. 1992). Titration was performed by titration with 0.02N HCl using green. Hardness and NH ₄ -N were analyzed according to the Test Method of Drinking Water Quality Process (Ministry of Environment. 1997). Was analyzed using ICP-MS (ELAN DRCe from PERKIN-ELMER).

The collected drinking water was filtered through a 0.2㎛ filter and used in the following experiment.

<Examples> Antidiabetic Efficacy Assessment

SFF rats (Wister, male), about seven weeks old, were purchased and were given a period of acclimation to the laboratory environment for more than three weeks with sufficient solid feed and water in the animal breeding room. After 10 days of adaptation, the rats were fasted for 12 hours, and then high blood glucose mice were induced by intraperitoneal administration of alloxan (Sigma, USA) at 185 mg / kg body weight. One day after the administration of aloxane, about 4 to 5 times higher blood sugar (400 to 480 mg / dL) was induced in the experiment compared to normal blood glucose (105 mg / dL) of the experimental animals. Breeding environment was maintained at a temperature of 23 ㅁ 2 ℃, a humidity of 50 ㅁ 5%, night and day every 12 hours.

The hyperglycemic rats were divided into 11 groups of 3 rats, and 10 experimental groups were allowed to freely consume 10 drinking waters of the reference example with a solid feed instead of a constant, and one control group was allowed to freely consume a constant with a solid feed.

The experiment was carried out over a total of 8 weeks, blood glucose was measured using a glucose strip (Glucocard ™ Test Strip II, ARK RAY, INC) by cutting about 1mm of the tail of the rat tail at a weekly interval and collecting a small amount of blood.

The results are shown in FIG. 1.

Referring to FIG. 1, it can be seen that in the administration group of the drinking water of the reference example, the blood glucose level is lowered with the administration group over time.

However, in the control group administered constant, all died on day 3 due to severe diabetes.

<Example Formulation Examples of Pharmaceutical Compositions and Beverage Compositions

Preparation Example 1 Preparation of Capsule

In the reference example, the drinking water of <S-10> in the drinking water was filtered with a 0.2 μm filter, and then concentrated by about 10 times as much as the raw material using a 20-l vacuum vacuum concentrator. The concentrated water was heated and dried to evaporate moisture and powder. Concentrated in form. Here, 3 g of the concentrated powder, 100 mg of corn starch, 100 mg of lactose and 2 mg of magnesium stearate were thoroughly mixed, and then filled into hard gelatin capsules according to a conventional method for preparing capsules to prepare capsules.

Preparation Example 2 Preparation of Functional Drink

Homogeneous formulations such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%) and water (75%) and 3 g of the concentrated powder obtained in <Example 1> After instant sterilization, it was packaged in small packaging containers such as glass bottles and plastic bottles to prepare health drinks.

1 is a result showing that the blood sugar of the experimental animal is lowered when the groundwater obtained by collecting and filtering in a specific 10 wells of Jeju Island to the diabetes-induced experimental animal freely ingested.

Claims (12)

An antidiabetic beverage composition comprising groundwater obtained by harvesting and filtering in a groundwater well identified in (1) to (10) below or a dehydrated form thereof as an active ingredient. (1) 5m above sea level, 60m above sea level, 2175-1 Sagye-ri, Andeok-myeon, Namjeju-gun, Jeju-do, Korea; (2) 5m above sea level, 396 Ongpo-ri, Hallim-eup, Bukjeju-gun, Jeju-do, 70m drilling depth; (3) 4m above sea level, 396 Ongpo-ri, Hallim-eup, Bukjeju-gun, Jeju-do, 70m excavation depth; (4) 37m above sea level, 344 Sangwi-ri, Aewol-eup, Bukjeju-gun, Jeju-do, 90m deep; (5) 295m above sea level, 640-1 Saekdal-dong, Seogwipo-si, Jeju-do, 301m; (6) 416m above sea level, No. 52, Saekdal-dong, Seogwipo-si, Jeju-do, 400m deep; (7) 275m above sea level, 2863 Wahul-ri, Jocheon-eup, Bukjeju-gun, Jeju-do, 315m with excavation depth; (8) 6.0m above sea level, 2172-1 Sagye-ri, Andeok-myeon, Namjeju-gun, Jeju-do, 67m deep; (9) 143m above sea level, 1257 Sangmyeong-ri, Hallim-eup, Bukjeju-gun, Jeju-do, 170m excavation depth; And (10) 271m above sea level, 2422-9 Seohong-dong, Seogwipo-si, Jeju-do 296m The method of claim 1, The dehydrated form is an anti-diabetic beverage composition, characterized in that the concentrated powder is completely evaporated moisture. The method of claim 1, Anti-diabetic beverage composition, characterized in that it comprises one or more selected from the sweeteners, flavors, physiologically active ingredients, minerals, preservatives, emulsifiers, acidulants and thickeners. The method of claim 3, The physiologically active substance is at least one selected from catechin, epicatechin, gallocatechin, epigallocatechin, retinol, ascorbic acid, tocopherol, calciferol, thiamine and riboflavin. The method of claim 2, The concentrated powder is an anti-diabetic beverage composition, characterized in that included in the beverage composition in the range of 0.001% to 10% by weight based on the total weight of the beverage composition. An antidiabetic pharmaceutical composition comprising a concentrated powder of groundwater obtained by collecting and filtering in an underground water well identified in (1) to (10) below. (1) 5m above sea level, 60m above sea level, 2175-1 Sagye-ri, Andeok-myeon, Namjeju-gun, Jeju-do, Korea; (2) 5m above sea level, 396 Ongpo-ri, Hallim-eup, Bukjeju-gun, Jeju-do, 70m drilling depth; (3) 4m above sea level, 396 Ongpo-ri, Hallim-eup, Bukjeju-gun, Jeju-do, 70m excavation depth; (4) 37m above sea level, 344 Sangwi-ri, Aewol-eup, Bukjeju-gun, Jeju-do, 90m deep; (5) 295m above sea level, 640-1 Saekdal-dong, Seogwipo-si, Jeju-do, 301m; (6) 416m above sea level, No. 52, Saekdal-dong, Seogwipo-si, Jeju-do, 400m deep; (7) 275m above sea level, 2863 Wahul-ri, Jocheon-eup, Bukjeju-gun, Jeju-do, 315m with excavation depth; (8) 6.0m above sea level, 2172-1 Sagye-ri, Andeok-myeon, Namjeju-gun, Jeju-do, 67m deep; (9) 143m above sea level, 1257 Sangmyeong-ri, Hallim-eup, Bukjeju-gun, Jeju-do, 170m excavation depth; And (10) 271m above sea level, 2422-9 Seohong-dong, Seogwipo-si, Jeju-do 296m The method of claim 6, The concentrated powder is an anti-diabetic pharmaceutical composition, characterized in that contained in the pharmaceutical composition in the range of 0.001% to 10% by weight based on the total weight of the pharmaceutical composition. Beverage composition for preventing or treating diabetic complications comprising groundwater obtained by collecting and filtering in groundwater wells identified in (1) to (10) below or a dehydrated form thereof as an active ingredient. (1) 5m above sea level, 60m above sea level, 2175-1 Sagye-ri, Andeok-myeon, Namjeju-gun, Jeju-do, Korea; (2) 5m above sea level, 396 Ongpo-ri, Hallim-eup, Bukjeju-gun, Jeju-do, 70m drilling depth; (3) 4m above sea level, 396 Ongpo-ri, Hallim-eup, Bukjeju-gun, Jeju-do, 70m excavation depth; (4) 37m above sea level, 344 Sangwi-ri, Aewol-eup, Bukjeju-gun, Jeju-do, 90m deep; (5) 295m above sea level, 640-1 Saekdal-dong, Seogwipo-si, Jeju-do, 301m; (6) 416m above sea level, No. 52, Saekdal-dong, Seogwipo-si, Jeju-do, 400m deep; (7) 275m above sea level, 2863 Wahul-ri, Jocheon-eup, Bukjeju-gun, Jeju-do, 315m with excavation depth; (8) 6.0m above sea level, 2172-1 Sagye-ri, Andeok-myeon, Namjeju-gun, Jeju-do, 67m deep; (9) 143m above sea level, 1257 Sangmyeong-ri, Hallim-eup, Bukjeju-gun, Jeju-do, 170m excavation depth; And (10) 271m above sea level, 2422-9 Seohong-dong, Seogwipo-si, Jeju-do 296m The method of claim 8, The dehydrated form is a beverage composition for preventing or treating diabetes complications, characterized in that the concentrated powder is completely evaporated moisture. 10. The method of claim 9, The concentrated powder is a composition for preventing or treating diabetic complications, characterized in that included in the beverage composition in the range of 0.001% to 10% by weight based on the total weight of the beverage composition. Pharmaceutical composition for preventing or treating diabetic complications comprising a concentrated powder of groundwater obtained by collecting and filtering in the groundwater wells identified in (1) to (10) below as an active ingredient. (1) 5m above sea level, 60m above sea level, 2175-1 Sagye-ri, Andeok-myeon, Namjeju-gun, Jeju-do, Korea; (2) 5m above sea level, 396 Ongpo-ri, Hallim-eup, Bukjeju-gun, Jeju-do, 70m drilling depth; (3) 4m above sea level, 396 Ongpo-ri, Hallim-eup, Bukjeju-gun, Jeju-do, 70m excavation depth; (4) 37m above sea level, 344 Sangwi-ri, Aewol-eup, Bukjeju-gun, Jeju-do, 90m deep; (5) 295m above sea level, 640-1 Saekdal-dong, Seogwipo-si, Jeju-do, 301m; (6) 416m above sea level, No. 52, Saekdal-dong, Seogwipo-si, Jeju-do, 400m deep; (7) 275m above sea level, 2863 Wahul-ri, Jocheon-eup, Bukjeju-gun, Jeju-do, 315m with excavation depth; (8) 6.0m above sea level, 2172-1 Sagye-ri, Andeok-myeon, Namjeju-gun, Jeju-do, 67m deep; (9) 143m above sea level, 1257 Sangmyeong-ri, Hallim-eup, Bukjeju-gun, Jeju-do, 170m excavation depth; And (10) 271m above sea level, 2422-9 Seohong-dong, Seogwipo-si, Jeju-do 296m The method of claim 11, The concentrated powder is a pharmaceutical composition for preventing or treating diabetic complications, characterized in that included in the pharmaceutical composition in the range of 0.001% to 10% by weight based on the total weight of the pharmaceutical composition.

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