KR20090132372A - Cosmetic composition for enhancing the skin permeability of the mineral components containing low molecular weight poly-gamma-glutamic acid - Google Patents

Abstract

PURPOSE: A cosmetic composition containing low molecular weight of poly-gamma-glutamic acid is provided to easily ensure skin permeation of mineral. CONSTITUTION: A cosmetic composition contains less than 1000Da of poly-gamma-glutamic acid and mineral. The poly-gamma-glutamic acid is contained in 0.1-20 weight% based on total weight. The mineral is calcium(Ca), soium(Na), potassium(K), zinc(Zn), iron(Fe), selenium(Se), or lithium(Li). The mineral is contained in 0.001-10 weight% based on total weight. The poly-gamma-glutamic acid is denoted by chemical formula 1. The cosmetic composition is used in the form of astringent, nutrition lotion, nutrition essence, nutrition cream, massage cream, essence, eye cream, cleansing water, pack or body essence.

Description

Cosmetic composition for enhancing the skin permeability of the mineral components containing low molecular weight poly-gamma-glutamic acid}

The present invention relates to a cosmetic composition containing low molecular weight polygammaglutamic acid and minerals to facilitate the skin absorption of minerals to enhance the skin absorption of minerals.

Poly-gamma glutamic acid (Poly-γ-glutamic acid, γ-PGA) is an anionic amino acid polymer in which the γ-carboxy group of glutamic acid and the α-amino group of glutamic acid are amide-bonded. It is produced from strains of the genus Bacillus isolated from Cheonggukjang, natto, a Japanese traditional soybean fermented food, and Kinema, a traditional soybean fermented food in Nepal. Polygamma glutamic acid produced from the Bacillus sp. Strain is an edible, water soluble, anionic and biodegradable material.

Recently, research on the production and use of poly-gamma glutamic acid has been actively conducted in developed countries, focusing on the development of alternative commodity materials of refractory polymers, heat-resistant plastics by esterification, and production of water-soluble fibers and membranes. In addition, research on the change of properties caused by gamma-ray irradiation of polygamma glutamic acid and the development and industrialization of hydrogels by crosslinking agents are being promoted.

Hydrogel is made of polygamma glutamic acid, a biopolymer produced by fermentation produced by Bacillus subtilis Chungkookjang, and is produced through intermolecular or intramolecular crosslinking, and is an environmentally friendly material having absorption, biodegradability and plasticity. Examples of the crosslinking method include irradiation with a gamma ray and an electron beam, a chemical crosslinking agent treatment method using an epoxy resin, and the like. Irradiation of the polygamma glutamic acid aqueous solution causes crosslinking reaction between the polygamma glutamic acid molecules, thereby obtaining a polygamma glutamic acid resin having absorbency, biodegradability and plasticity.

Effect of Manganese Ion on the Composition and Production of Polygammaglutamic Acid, Study on the Use of Water Soluble Polymer by Ultrasonic Decomposition and Development of Low Water Soluble Plastic by Synthesis of Ester Derivatives (Biosci. Biotechnol. Bochem., 60 (8): 1239-42, 1996) and polygamma glutamic acid production by Bacillus subtilis and its utilization as health care foods with osteoporosis treatment effect as calcium dissolving agent (JP-A-632742), promoting mineral absorption Polygamma glutamic acid-containing compositions (Japanese Patent Application Laid-open No. 5-316999) and the like have been reported as raw materials.

In addition, the effect of reducing water pollution by reducing the phosphorus content of water-based (European Patent No. 838160), and use as a solidified biodegradable fiber, film and film molded body by dissolving, precipitation and drying of polygamma glutamic acid (Japanese Patent Publication) Patent No. 6-92870: Japanese Patent Application Laid-Open No. 6-256220) has also been published.

In addition, patents and high molecular weights for efficient production of polygamma glutamic acid (Korean Patent Application No. 1997-3404, Korean Patent Application No. 1997-67605) and high concentration polygamma glutamic acid production method (Korean Patent Application No. 2001-0106025) A patent has also been filed for the salt tolerant strain Bacillus subtilis Chungkukjang (International Application PCT / KR01 / 01372) that produces polygammaglutamic acid.

Meanwhile, a patent has been filed for a food (Korean Patent Application No. 2005-0006910 and European Patent No. 00605757) related to promoting absorption of ultra high molecular weight polygamma glutamic acid (PGA) and minerals containing minerals in the body.

The molecular weight of polygamma glutamic acid, which has been used as a mineral absorption promoting material in the past, has an application aspect as a food material in the body with a molecular weight of 10,000 Da or more, but application to the skin has not been reported.

Accordingly, the inventors of the present invention have applied the low-molecular weight polygamma glutamic acid to promote absorption in the skin, and have confirmed that the mineral absorption effect in the skin is excellent.

Accordingly, it is an object of the present invention to provide a cosmetic composition capable of promoting skin absorption of minerals by containing low molecular weight polygammaglutamic acid having a molecular weight of 1000 Da or less together with minerals.

In order to achieve the above object, the present invention provides a cosmetic composition containing a poly gamma glutamic acid and a mineral having an average molecular weight of 1000 Da or less.

Polygamma glutamic acid having a molecular weight of 1000 Da or less contained in the composition according to the present invention is a substance having high skin permeability, and acts as a skin absorption accelerator of minerals. The composition containing poly gamma glutamic acid facilitates skin absorption of minerals It can be usefully used as a skin external preparation composition or a cosmetic composition for enhancing skin absorption.

The cosmetic composition of the present invention contains a low molecular weight polygamma glutamic acid and a mineral having an average molecular weight of 1000 Da or less represented by the following formula (1).

Poly-gamma glutamic acid (γ-PGA) contained in the present invention is an anionic amino acid polymer having an amide-bonded γ-carboxyl group of glutamic acid and an α-amino group of glutamic acid having an average molecular weight of 1000 Da (molecular weight). 300 ~ 1200Da), which collects minerals and is used as a skin absorption accelerator.

In addition, the low molecular weight polygamma glutamic acid is contained in an amount of 0.1 to 20% by weight based on the total weight of the composition, when the content exceeds 20% by weight may cause skin safety problems. .

As shown in FIG. 1, the lipid gap of the skin cells is about 100-150 nm, and the low molecular weight polygamma glutamic acid (1000 Da) has a size of 10 nm or less. Therefore, the low molecular weight polygamma glutamic acid used in the present invention has the property of being easily permeable through the cell gap and absorbing up to the dermis. In addition, polygamma glutamic acid has abundant negative charge, and thus has excellent electrical conductivity, so it has a characteristic of smoothly penetrating minerals into the skin having a positive charge.

The minerals used in the present invention are essential for the living body and are necessary for skin improvement, moisturizing, and the like, and the minerals that can be used are not limited thereto, but preferably calcium (Ca), sodium (Na), and magnesium (Mg). ), Potassium (K), zinc (Zn), iron (Fe), selenium (Se) and lithium (Li) at least one selected from the group containing.

Calcium is involved in improving barrier function, strengthening cell membranes and bones, and in cardiac muscle and nerves. It is also necessary for the formation of strong keratinocytes when the granule cells are keratinized into keratinocytes in the skin. Sodium is involved in maintaining proper fluids in the body, strengthening muscle activity, regulating body fluids, controlling osmotic pressure, and maintaining electrolyte balance in the body. Magnesium helps transport the intercellular lipids produced by the granular layer to the stratum corneum and promotes the recovery of wounded skin protection, promotes cell metabolism, activates the effects, and is involved in autonomic nerve regulation. Potassium is involved in the improvement of barrier function and also in metabolism by releasing waste products out of the body through water retention. Zinc promotes skin metabolism and helps maintain elasticity. Iron carries enzymes, binds collagen and is involved in energy metabolism. Selenium is an antioxidant involved in the detoxification of heavy metals and cell membrane maintenance. Lithium is involved in activating reproductive function and normalizing blood pressure, and is also used in chemotherapy and neuralgia.

In the present invention, the mineral is contained in an amount of 0.001 to 10% by weight based on the total weight of the composition. The content of more than 10% by weight does not affect the efficacy in the skin, but may have problems with skin permeation.

The low molecular weight polygamma glutamic acid used in the present invention may be prepared using a strain or may also be prepared by a chemical method, but is not limited thereto .

The cosmetic composition of the present invention uses a low molecular weight polygamma glutamic acid to permeate essential mineral components that help skin improvement, and is not particularly limited in the formulation thereof. For example, soft cosmetics and astringent cosmetics , Nourishing lotion, nourishing lotion, nourishing essence, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, gel, powder, makeup base, body lotion, body cream, body oil It may be formulated into a cosmetic composition, such as body essence, and may also be a cosmetic composition having a transdermal permeable formulation such as lotion, ointment, gel, cream, patch or spray.

In addition, other components in each formulation may be appropriately selected and blended by those skilled in the art without difficulty depending on the type or purpose of external preparation.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, and the scope of the present invention is not limited by the examples.

Reference Example Preparation of Low Molecular Weight Polygamma Glutamic Acid

Low molecular weight polygamma glutamic acid having an average molecular weight of 1000 Da was prepared by the cultivation of the saline-resistant strain Bacillus subtilis var chungkookjang (KCTC 0697BP) isolated from Korean traditional soybean fermented food. Polygamma glutamic acid manufactured by Bio Leaders was used.

[Test Example 1] A skin absorption promoting effect of the mineral components trapped in low molecular weight polygamma glutamic acid

It was prepared in the form of an aqueous solution containing 1% by weight of low molecular weight polygamma glutamic acid and minerals, and then tested the effect of promoting mineral absorption in the skin.

Polygammaglutamic acid having a molecular weight of 1000 Da having a concentration of 1% by weight and a solution containing calcium, magnesium, potassium, zinc and iron in minerals were mixed and applied to the hairless mouse skin by 1 ml. Twenty 6-12 week old male hairless mice were used. The mice were divided into two groups of 10 mice each. Group 1 was coated with an aqueous mineral solution containing 1% by weight of polygamma glutamic acid having a molecular weight of 1000 Da as a test group. As a control, an aqueous mineral solution containing no polygammaglutamic acid was applied.

For each group an aqueous mineral solution was applied to the skin twice a day. One week after application, mouse skin tissues of the control and experimental groups were extracted with a diameter of 8 mm, and then the skin tissues were cut at intervals of 20 mm thickness from the epidermis and micro-particle induced X-ray emission (Micro-PIXE) using a 3 MeV proton beam. Minerals were quantitatively analyzed. Micro-PIXE technology uses a SI (Li) detector to receive spectra of certain x-rays generated by external electrons filled with excited cabinet electrons when an ion beam is irradiated onto a sample. Na) It is an analytical device for quantitative analysis of the above elements.

The results analyzed by the above method are shown in Table 2 below.

Comparison of mineral content according to skin thickness after polygamma glutamic acid absorption (unit: ppm) Skin thickness Control Experimental group Ca Mg K Zn Fe Ca Mg K Zn Fe 0-20mm 125 87 63 12 37 117 92 71 11 28 20-40 mm 105 45 38 2 15 142 84 68 12 26 40 ~ 60mm 73 46 29 4 16 125 79 72 9 26 60 ~ 80mm 47 37 27 2 12 105 72 57 5 23 80-100mm 32 27 16 5 13 85 54 62 7 18

Looking at Table 2, in the case of the control group, the number of minerals present in the skin is the highest when the thickness of the skin is 0 to 20 mm, and when the thickness is exceeded, the number of minerals present rapidly decreases. On the other hand, when it contains a low molecular weight polygamma glutamic acid it can be confirmed that the mineral content according to the skin thickness is hardly reduced.

Therefore, it can be seen from the above results that low-molecular weight polygamma glutamic acid has an excellent effect of promoting mineral absorption of the skin, and low-molecular weight polygamma glutamic acid can be utilized as a cosmetic product that enhances skin absorption of minerals. It can be said that.

Formulation Example 1 Nutritious Longevity (Milk Lotion)

Nutrients containing polygamma glutamic acid can be prepared according to a conventional method as shown in the following table.

Ingredient weight% γ-PGA 1% mineral solution 2.0 Squalane 5.0 Beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Caprylic / Capric Triglycerides 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity Purified water to 100

Formulation Example 2 Softener (Skin Lotion)

The flexible longevity containing polygamma glutamic acid can be manufactured according to a conventional method as shown in the following table.

Ingredient weight% γ-PGA 1% mineral solution 2.0 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxy Vinyl Polymer 0.1 PEG 12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, flavoring Quantity Purified water to 100

Formulation Example 3 Nutrition Cream

Nutritive creams containing polygamma glutamic acid can be prepared according to a conventional method as shown in the following table.

Ingredient weight% γ-PGA 1% mineral solution 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 PEG60 Cured Castor Oil 2.0 Liquid paraffin 10 Squalane 5.0 Caprylic / Capric Triglycerides 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 antiseptic Quantity Pigment Quantity Spices Quantity Purified water to 100

Formulation Example 4 Massage Cream

Massage creams containing polygamma glutamic acid can be prepared according to a conventional method as shown in the following table.

Ingredient weight% γ-PGA 1% mineral solution 1.0 Beeswax 10.0 Polysorbate 60 1.5 PEG 60 Cured Castor Oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / Capric Triglycerides 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity Purified water to 100

[Formulation Example 5] Pack

Packs containing polygamma glutamic acid can be prepared according to conventional methods as shown in the following table.

Ingredient weight% γ-PGA 1% mineral solution 1.0 Polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 PEG 12 nonylphenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, flavoring Quantity Purified water to 100

Formulation Example 6 Gel

As shown in the following table, a gel containing polygamma glutamic acid may be prepared according to a conventional method.

Ingredient weight% γ-PGA 1% mineral solution 2.0 Ethylenediamine Sodium Acetate 0.05 glycerin 5.0 Carboxy Vinyl Polymer 0.3 ethanol 5.0 PEG 60 Cured Castor Oil 0.5 Triethanolamine 0.3 Preservative, coloring, flavoring Quantity Purified water to 100

Formulation Example 7 Water-in-oil Emulsion Makeup Cosmetics

As shown in the following table, the oil-deposited emulsion cosmetic containing polygamma glutamic acid can be prepared according to a conventional method.

Ingredient weight% γ-PGA 1% mineral solution 3.0 Butylene Glycol 10 Salt 1.5 Ozokerite 2.0 Dicaprylylcarbonate 20 Sorbitan isostearate 2.5 Cetyl Dimethicone Copolyol 2.0 Polymethyl methacrylate 3.0 Iron oxide 1.5 Titanium oxide 12.0 Talc (size: 3.06 micro) 5.0 Preservative, coloring, flavoring Quantity Purified water to 100

Formulation Example 8 Twin Cake Powder Powder

Twin cakes and powder cosmetics containing polygamma glutamic acid can be prepared according to conventional methods as shown in the following table.

Ingredient weight% γ-PGA 1% mineral solution 1.0 Nylon powder 5.0 Modified Starch 3.0 Titanium dioxide 3.0 Mica 25.0 Squalene 0.5 Dimethicone 0.8 Preservative, coloring, flavoring Quantity Siliconized Talc to 100

1 is a schematic diagram of skin absorption of low molecular weight polygamma glutamic acid having a molecular weight of 1,000 Da or less.

Claims (5)

Cosmetic composition containing polygamma glutamic acid and mineral whose average molecular weight is 1000 Da or less. The cosmetic composition according to claim 1, wherein the polygamma glutamic acid is contained in an amount of 0.1 to 20% by weight based on the total weight of the composition. The method of claim 1, wherein the mineral is calcium (Ca), sodium (Na), magnesium (Mg), potassium (K), zinc (Zn), iron (Fe), selenium (Se) and lithium (Li) Cosmetic composition, characterized in that at least one selected from the group. The cosmetic composition according to claim 1, wherein the mineral is contained in an amount of 0.001 to 10% by weight based on the total weight of the composition. The cosmetic composition according to claim 1, wherein the polygamma glutamic acid is used as a skin absorption promoter of minerals.

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