KR20090096882A - A free radical removal composition comprising naphthoquinone-pigment and extract of mountain panax ginseng c. a. mayer adventitous roots and cigarette comprising the composition - Google Patents

Abstract

A composition for removing free radical, which a naphthoquinone colorant and ginseng root cultured extract and a cigarette containing the same are provided to completely remove free radical in smoke of cigarette. A composition for removing free radical comprises a naphthogquinone colorant and ginseng root cultured extract as active ingredients in the weight ratio of 1 : 0.01~1.5. A free radical in main stream of cigarette is measured through spin trapping technique using PBN (alpha-phenyl-N-tert-butyl nitrone).

Description

A free radical removal composition comprising naphthoquinone-pigment and extract of Mountain Panax ginseng CA Mayer containing naphthoquinone pigments and wild ginseng root extracts with excellent free radical removal ability as active ingredients adventitous roots and cigarette comprising the composition}

TECHNICAL FIELD The present invention relates to a cigarette filter technology for removing free radicals, which are harmful components of cigarette smoke.

Globally, smoking has been a major problem for health. Tobacco smoke has been found to contain as many as 4000 kinds of carcinogens and toxic chemicals.

The main component of tobacco smoke is first nicotine. Nicotine is a medically classified substance because it causes almost the same level of addictive addiction as opiates. Small amounts of nicotine act on the nervous system to excite the sympathetic and parasympathetic nerves to get pleasure, and high amounts paralyze the nerves leading to hallucinations. Nicotine constricts peripheral blood vessels, speeds up the pulse, increases blood pressure, and increases cholesterol, making arteriosclerosis worse. That's why heart smokers, Burger's disease, and frostbite are so common in smokers. In addition, nicotine acts on the digestive system, causing ulcers and adversely affecting the endocrine system and respiratory system.

Second, it is burning. Tar is a poisonous substance, commonly called tobacco, produced by the burning of any plant and contains thousands of toxic chemicals in it. Most of the detoxification of tobacco to our health is due to the various toxic and carcinogens in this tar. Tar itself is highly toxic and can kill small animals and insects in small amounts, so it was previously used to collect cigarette butts to prevent the worms from sinking in the toilet and to combat snakes in the mountains. The unique taste of tobacco comes from this tar, and the tar enters the lungs through tobacco smoke, penetrates into the blood, damaging all the cells of our body and all organs, and acts directly on the gums and bronchus to cut epidermal cells. It can destroy or cause chronic inflammation. Tar, which is contained in domestic and foreign cigarettes (1 piece), is also sold in Korea, and the oxygen transport capacity of blood is reduced, causing chronic hypoxia, which impairs metabolism and causes premature aging.

Third is carbon monoxide. Carbon monoxide (CO) is already known to us for its anthracite odor. If you smoke a lot or stay in a room full of cigarette smoke for a long time, your head hurts and your mind is depressed because of carbon monoxide. It acts as a temporary arousal and soothing effect, increases pulse and blood pressure, causes peripheral blood vessel contraction and atherosclerosis. Other harmful components include arsenic, ammonia, butane, cadmium, carbon monoxide, cyanide, formaldehyde, methanol, and the like. Recently, it has been found that free radicals, such as free radicals in tobacco smoke, which have very high chemical reactivity, play a more critical role in health. In addition, when non-smokers are exposed to tobacco smoke in the air, lung cancer, laryngeal cancer, and other cancers, like smokers, have a significantly higher incidence of diseases of the respiratory system and cardiovascular system.

Since smoking has a strong habit in itself, smokers cannot simply quit smoking. Therefore, with the efforts to increase the smoking cessation environment, research has been conducted to eliminate the harmful effects of smoking. Many techniques for removing harmful substances contained in cigarette smoke have been proposed. In particular, a number of cigarette filters have been developed and used that can remove the tar component of the main harmful component of the smoke inhaled during smoking. In addition to the direct installation in cigarettes, a smoking apparatus has also been developed which is disposed on a desk, for example, to suction off the smoke so that smoke does not diverge to the surroundings.

Recently, as the mechanism at the molecular level of various diseases has been revealed, it is confirmed that the main cause of lung cancer due to smoking is free radicals with high chemical reactivity such as free radicals in tobacco smoke. However, there is no effective technique for removing free radicals having high chemical reactivity in cigarette smoke.

Y. Niwano et al. Is a free radical scavenger that uses a naphthoquinone pigment, shikonin, or an isomer of alkannin, or a mixture of siconin and alkanine. Was developed and obtained a patent (Japanese Patent Application No. 2001-301811). However, the removal effect of the free radicals of naphthoquinone pigments is not so great.

Free radicals, such as free radicals in tobacco smoke, have been found to play a more critical role in health, and when non-smokers are exposed to tobacco smoke in the air, they are the same as smokers, lung cancer, laryngeal cancer, and other respiratory organs. Since the incidence of lineage and cardiovascular disease has risen significantly and the social costs are so great, there has been a demand for the complete elimination of free radicals in tobacco smoke.

The inventors of the present invention, when mixed with naphtho-quinone pigments (mountain Panax ginseng CA Mayer adventitious roots extract), nakontoquinone pigments (shikonin) or isomers of alkannin (alkannin) or sikonin and alkanes ESR spectrometry quantitative analysis showed that the free radical scavenging ability was superior to the mixture of nin (Ref .: Mohammad Babar Ali, Nisha Singh, Abdullah Mohammad Shohael, Eun Joo Hahn and Kee-Yoeup Paek Plant Science 2006, 171). , 147-154; Mohammad Babar Ali, Kee-Won Yu, Eun Joo Hahn and Kee-Yoeup Paek Plant Science 2005, 169, 83-92; Mohammad Babar Ali, Nguyen Trung Thanh, Kee-Won Yu, Eun Joo Hahn, Kee -Yoeup Paek, Hyung Lae Lee Plant Science 2005, 169, 833-841 Mohammad Babar Ali, Eun Joo Hahn and Kee-Yoeup Paek Plant Physiology and Biochemistry 2005, 43, 449-457. The present inventors have conducted many experiments in consideration of free radical scavenging ability and economical aspects, and with mountain panax ginseng CAMayer adventitious roots extract and shikonin or its isomers alkannin or sikonin. It has been found that the mixture of alkanins is much better as a free radical scavenger in tobacco free radical scavenging filters.

According to the present invention, it is possible to completely remove free radicals in cigarette smoke. In particular, by using a wild ginseng culture root extract and siconin or alkane as a free radical removal material, it is also possible to visually confirm the removal ability of the free radical by color change.

It is an object of the present invention to provide a composition for removing free radicals containing naphthoquinone-based pigment and wild ginseng cultured root extract as active ingredients.

Another object of the present invention is to provide a cigarette containing a composition for removing free radicals containing naphthoquinone-based pigment and wild ginseng cultured root extract as an active ingredient in the filter layer or the next layer of the cigarette in tobacco.

Still another object of the present invention is to provide that the cigarette filter is arranged so as to pass in the order of the tar removal filter and the free radical removal filter to remove tar in the smoke of the cigarette.

It is still another object of the present invention to provide a filter for free radical removal, which is configured so that the free radical removal ability can be visually identified as a change in color tone.

The free radical removal filter contains free radical extract and wild ginseng root extract and colored substances with free radical removal. When the smoke of the cigarette passes through the filter, the free radicals are captured by the free radical removing material. Especially. When the free radicals are captured by the free radical removing material of the naphthoquinone pigments composed of the coloring solution, the color gradually becomes thinner, and the removal state of the free radicals of the filter can be known. For example, it can be judged that there is no free radical removal ability of the filter at the time of colorlessness.

As a harmful ingredient in conventional tobacco smoke, the focus has been mainly on chemicals such as benzopyrene in tar, but it is the free radical that is highly chemically reactive such as active oxygen in tobacco smoke. Turned out. In particular, the mechanism of lung cancer may differ from case to case, but in general, it is estimated that free radicals are the main cause. Therefore, the development of a technique for removing free radicals in the smoke during smoking has emerged as a very important problem.

It is an object of the present invention to remove free radicals from tobacco smoke using wild ginseng cultured root extract and colored substances with free radical removal function which have a homogeneous effect as a substance having superior free radical removal efficiency and less toxicity than conventionally known compounds. To provide for the development of tobacco filters. In addition, it was thought that it is necessary to remove the free radicals so that the removal ability can be visually identified.

In order to achieve the above object, the present invention provides a tobacco filter for removing free radicals by using a color ginseng extract and a free radical removing function as an active ingredient:

Naphthoquinone pigments used in the present invention include siconin, alkane and mixtures thereof.

Naphthoquinone pigment and wild ginseng cultured root extract in the present invention is naphthoquinone pigment: wild ginseng cultured root extract is 1: 0.01 ~ 1.5 by weight ratio. The naphthoquinone pigment and wild ginseng cultured root extract preferably contain 0.001 mg to 0.01 mg of a naphthoquinone pigment: wild ginseng cultured root extract in a weight ratio of 1: 0.01 to 1.2 with respect to one of the conventional cisterns.

The composition of the present invention can be prepared by dissolving the composition of the present invention in a cigarette of a tobacco cigarette, a filter for removing tar, or a filter after removing the tar, and depositing and drying the composition.

Next, the present invention will be described in more detail with reference to Examples.

Example  One

Figure 1 is a cross-sectional view showing the cigarette filter 2 attached to cigarette 1. As shown in FIG. 1, the tobacco filter 2 has a tar removal filter 3 directly connected to the tobacco body, and the filter 4 for removing free radicals is installed in series. In this way, during smoking, tobacco smoke passes through the tar removal filter 3 and thereafter passes through the free radical removal filter 4. The tar removal filter 3 may use a tar removal filter currently in use.

The free radical removal filter 4 is configured to have wild ginseng culture root extract and siconin (or alkane) in the filter base material. Since the ability to remove free radicals is in both siconin and alkane, either the siconin and the alkane may be separated and included in the filter base material, or may be included in a mixed state without separation. Siconin is a purple plate crystal and alkane is a reddish brown needle crystal. In the case of containing the wild ginseng culture root extract and the siconin (or alkanin) as the filter substrate, when the hydrophilic polymer is used as the filter substrate, good color development can be obtained, and the removal of free radicals due to color change is easy to be confirmed.

In the case of smoking a cigarette having a cigarette filter 2 of this configuration, the smoke is removed from the tar removal filter 3 tar, and then passes through the free radical removal filter 4 to remove the tar and free radicals. Smokers inhale the smoke from tar and free radicals, thereby suppressing the occurrence and exacerbation of various diseases caused by tar and free radicals.

Since the filter 4 for free radical removal according to the present invention has a composition in which the wild ginseng culture root extract and the siconin or alkanine are included in the filter material as described above, the red-reddish purple is initially colored, but as the free radical is removed, It becomes colorless because it changes to a stable semiquinone type. Therefore, in the free radical removal filter 4, the original red-red color turns into the color of the filter base material or the color in which tar etc. were adsorb | sucked to some extent to the filter base material. This color tone depends on the amount of adsorption of tar or the like and the original color tone of the filter material, but when the color tone uses an off-white filter material, this color tone can be sufficiently determined by eye.

Example  2

Free radicals in the gaseous state in the main stream were measured by ESR using spin trapping technique using PBN ( α -phenyl- N - tert- butyl nitrone).

Before collecting the free radicals of the tobacco, it was stored for 2 days in a constant temperature and humidity room (67% humidity, 22 ^° C.) to equalize the conditions of the experimental tobacco. Tobacco filter was prepared by administering sikonin, wild ginseng culture root extract and siconin and wild ginseng culture root extract at 20% concentrations of 0%, 0.01%, 0.05% and 0.1%, respectively.

The administered concentration was defined as {mg of ciconin or wild ginseng cultured root extract [% of tobacco weight (670 mg)] / ethanol 100 μL}. 0.1M PBN hexane solution was prepared to perform spin trapping experiment. 5 mL of 0.1M PBN hexane solution was added to the spin trap device, and 4 spin traps were connected in series. 20 cigarettes of different concentrations were smoked at the same time using a rotary type machine, and the mainstream smoke of the filtered cigarette was poured into 5 mL of 0.1 M PBN hexane solution and bubbled into a spin trap solution in which 4 spin traps were connected in series. The amount and time of inhaling cigarette smoke shall be inhaled in 35 cm ² for 2 seconds in accordance with international regulations and repeated for 58 seconds until the butt is approximately 32 mm (29 mm in filter part + 3 mm in cigarette). Burned.

A certain amount (400 μL) of this solution was placed in a quartz ESR tube and measured by electron spin resonance (ESR). ESR parameter is FREQ: 9.452150 [GHz], Field Center: 338.580 [mT]; width: 1.0 × 10 [mT], MOD: 100 [KHz]; width: 0.32 × 1 [mT], TIME Constant: 0.03 [sec], PHASE: 0 [deg], Power: 1.00 [mw], Sweep time: 0.5 [min], ACCUM: 1, Amplitude: 1 × 100 Measured.

As a result, when the ratio of 0.1% siconin: 0.1% wild ginseng culture root extract was administered as (1: 2, 2: 1, 1: 1), free radical removal efficiency was higher than that of using 0.1% siconin alone at 1: 1. This is about 20% improvement.

Example  3

Free radicals in the gaseous state in the main stream were measured by ESR using DPPH (1,1-diphenyl-2-picrylhydrazyl).

Before collecting the free radicals of the tobacco, it was stored for 2 days in a constant temperature and humidity room (67% humidity, 22 ^° C.) to equalize the conditions of the experimental tobacco. Tobacco filter was prepared by administering sikonin, wild ginseng culture root extract and siconin and wild ginseng culture root extract at 20% concentrations of 0%, 0.01%, 0.05% and 0.1%, respectively. The administered concentration was defined as {mg of ciconin or wild ginseng cultured root extract [% of tobacco weight (670 mg)] / ethanol 100 μL}. 1.0 mM DPPH ethanol solution was prepared. 5 mL of 1.0 mM DPPH ethanol solution was added to the spin trap device, and 4 spin traps were connected in series. 20 cigarettes of different concentrations were smoked at the same time using a rotary type machine, and the mainstream smoke of the filtered tobacco was added to 5 mL of 1.0 mM DPPH ethanol solution and bubbled into a spin trap solution in which 4 spin traps were connected in series. The amount and time of aspirating cigarette smoke shall be 35 cm ² for 2 seconds and repeat the pause for 58 seconds in accordance with international regulations, but the length of the butt will be about 32 mm (29 mm of cigarettes + 3 mm of tobacco). Burned up. A certain amount (400 μL) of this solution was placed in a quartz ESR tube and measured by electron spin resonance (ESR). ESR parameter is FREQ: 9.452150 [GHz], Field Center: 338.580 [mT]; width: 1.0 × 10 [mT], MOD: 100 [KHz]; width: 0.32 × 1 [mT], TIME Constant: 0.03 [sec], PHASE: 0 [deg], Power: 1.00 [mw], Sweep time: 0.5 [min], ACCUM: 1, Amplitude: 1 × 100 Measured.

As a result, when the ratio of 0.1% siconin: 0.1% wild ginseng culture root extract was administered as (1: 2, 2: 1, 1: 1), free radical removal efficiency was higher than that of using 0.1% siconin alone at 1: 1. This is about 25% improvement.

1 is a cross-sectional view showing the configuration of the cigarette filter of the present invention.

* Explanation of symbols on the main parts of the drawings *

1. Tobacco

2. Cigarette filter

3. Tar removal filter

4. Filter for free radical removal

Claims (4)

It is a composition that is effective in removing free radicals containing naphthoquinone pigment and wild ginseng cultured root extract as active ingredients. The composition for removing free radicals according to claim 1, wherein the naphthoquinone-based pigment and wild ginseng cultured root extract are contained in a weight ratio of 1: 0.01 to 1.5 by weight. The composition according to claim 1 or 2, wherein the naphthoquinone pigment is shikonin, alkannin or a mixture thereof. A tobacco cigarette containing a composition for removing free radicals prepared by dipping or spraying the composition of claim 1 onto a filter which is connected to a filter for removing tar or in a tar removing filter or a filter for removing tar.

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