TWI243027B - A cigarette filter with scavenging effect on free radicals in cigarette smoke and its preparation method - Google Patents

Abstract

A cigarette filter that has a scavenging effect on smoking induced gas phase free radicals. The filter ingredients are comprised of proanthocyanidins and include, but are not limited to, extracts of barks of pine tree, extracts of cones of cypress trees, extracts of grape seeds, and any combination thereof. Also, vitamin C and other known antioxidant ingredients may be added.

Description

A7 1243027 ____B7__ 5. Description of the invention (丨) Background of the invention:

FIELD OF THE INVENTION The present invention relates to an improved cigarette filter having an eradicating effect on gas-phase free radicals in cigarette smoke. The present invention uses filters that contain proanthocyanidins to scavenge free radicals. The present invention also relates to a method for manufacturing an improved cigarette filter having an eradicating effect on gas-phase radicals. It is well known that light smoke generates a large number of free radicals, including gas phase and solid phase free radicals. It is estimated that the number of gas-phase free radicals in each cigarette is 1015, which are mainly free radicals such as alkyl, alkoxy, peroxy, and nitric oxide (NO ·). It is known that the human body inhales gas-phase free radicals generated by cigarette smoke, which can cause toxicological and pathological changes harmful to the human body. It is also known that gas phase radicals are more dangerous to the human body than solid phase radicals. This is partly due to the high energy level, that is, the volatility of gas-phase free radicals. In particular, cigarette combustion involves a complex combustion process that generates free radicals contained in the smoke. Cigarette smoke is a complex mixture of more than 4,700 chemicals containing high concentrations of highly reactive free radicals that play a major role in smoke toxicity. Their free radicals directly or indirectly attack cellular components, and these free radicals are generally considered to be a cause of tobacco smoke-related diseases. Many parts of the body may be adversely affected by gas-phase free radicals, including the lungs, mouth, pharynx, esophagus, heart and circulatory system, and various organs. Free radicals can change the molecular structure of cellular proteins and lipids, cause DNA sequence breaks and cause mutations, thus increasing the risk of developing various types of cancer. Research shows that mainstream smoke (that is, smoke directly inhaled by light smoke) 3 This paper size applies to China National Standard (CNS) A4 (21 × x297 mm) (Please read the precautions on the back before filling out this Page)-^ 1 ^ 1 ϋ n H ϋ n 一 (OJ nn I mm§§ ϋ · ϋ II · A7 1243027 V. Description of the invention (>) (Please read the precautions on the back before filling this page) and side Stream smoke (smoke from the smoldering tobacco between each smoke and all exhaled smoke emitted by the smoker) contains high concentrations of free radicals. Sidestream smoke affects smokers and non-smokers around them. Smokers. The main health issues of non-smokers (including young children and children) are related to exposure to tobacco smoke from homes and other places issued by smokers. Do not smoke, but people exposed to second-hand sidestream smoke may suffer from The effects of free radical damage from tobacco smoke. Most of the free radicals in the gas phase of smoke produced by burning cigarettes are transient and unstable. Therefore, it is impossible to directly use electron spin resonance spectroscopy ("ESR spectroscopy") technology. In order to observe gas-phase free radicals, such as those found in cigarette smoke, spin capture technology is used. In this technique, gas-phase free radicals are captured and then converted into a self-test that can be tested by ESR spectroscopy. Spin adducts. A spin collector (PBN) collects smoke-gas free radicals, which are mainly alkoxy radicals (RO ·) and alkyl radicals (R ·). Gases in tobacco smoke Most of the phase radicals are RO · and alkyl R · radicals. During the combustion of cigarettes, nitrogen-containing substances oxidize and generate a large number of NO radicals (N Ο ·). Ν Ο · reacts with oxygen to produce reactive N 02 · Free radicals. N 02 · Free radicals can react with olefins (a substance produced during the combustion of cigarettes) to form alkoxy radicals RO ·, which may attack cell membranes and cause lipid peroxidation. Subsequently This lipid peroxidation may stimulate the release of oxygen free radicals from giant cells. Oxygen free radicals may cause damage to cell components. Their free radicals may poison cells and may be associated with the freedom of being stored in light smoke. Together Cancer and 4 This paper size applies Chinese National Standard (CNS) A4 specification (210 X 297 mm) A7 1243027 ______B7______ 5. Description of invention (发明) (Please read the notes on the back before filling this page) Heart disease. These Free radicals may also attack the lung anti-protease (which inhibits elastase), thereby rendering the lung τ-1 anti-protease inactive and thus causing lung injury. Free radicals from cigarette smoke and lungs induced by smoking are also considered Diseases such as emphysema, lung cancer, and heart disease are related. The components of the lung matrix (such as collagen and elastin) may be damaged and interrupted by oxidants in cigarette smoke. Damage from free radicals from cigarettes is not limited to the lung airways. Smokers' urine has been found to contain typical biological indicators of oxidative damage 10 times higher than the urine of non-smokers. The harmful pro-oxidant effects of smoking may even extend from the epicardial artery to the coronary microcirculation and affect the regulation of myocardial blood flow, resulting in thickening of the carotid artery middle layer. A filter claiming to eliminate free radicals in cigarette smoke was co-investigated by the Biophysics Institute of Academica Sinica and Beijing Cigarette Factory in 1995. This type of filter uses tea polyphenols, vitamin C and activated carbon as composite filters. This filter removes about 14% of gas-phase free radicals generated by cigarette smoke. If other ingredients including ematin, rutin, catechin and neorutin are added to cigarette tobacco, about 12% of gas-phase free radicals can be eliminated. The combination of these additional ingredients is called "kendir," and "Apocynum venetum L." Other cigarette filters that can eliminate free radicals are provided by Greece Golden Filter. Company) and Filter Development Company in 1999 ("5 paper sizes for joint development are applicable to the Chinese National Standard (CNS) A4 specification (210 X 297 mm) Α7 I i 1243027 '^ -------- V. Description of the invention (γ) filter, nozzle "). This filter includes activated carbon and heme. Its statement can eliminate about 90% of the gas phase freedom found in cigarette smoke These two filters are not commercially accepted by cigarette manufacturers. There are two main reasons for the poor commercial acceptance of these filters. One is that the large dose of additives in these filters reduces the cigarette Original smoke flavor. This is extremely detrimental to cigarette factories, because the taste and flavor of cigarettes are the main selling characteristics of recognized cigarette brands. Another reason is that it costs a lot of money to improve the equipment when manufacturing these composite filters. Cigarette Manufacturers are reluctant to invest in such improvements. Another filter disclosed in U.S. Patent No. 5,829,449 relates to the use of L-glutathione and a selenium source as free radical scavengers. Accordingly, 'required: i) A cigarette filter with good elimination effect on gas-phase free radicals in cigarette smoke, ii) A cigarette filter that can eliminate gas-phase free radicals in cigarette filters without significantly changing or reducing the flavor and taste of cigarettes Cigarette filters, and iii) a cigarette filter containing a free radical scavenger that can be moderately exposed to cigarette smoke to produce the maximum elimination effect in a short period of time. SUMMARY OF THE INVENTION One aspect of the present invention resides in An improved cigarette filter having an eradication effect on gas-phase free radicals induced by smoking, the radical eradication effect is achieved by adding an effective amount of a filtering ingredient or a filtering ingredient with vitamin C and / or technology Other ingredients known to have antioxidant filtering properties, but without a specific amount of L-glutathione. This filtering ingredient is a group consisting of proanthocyanidins (which may contain proanthocyanidins) Selected: These ingredients 6 (Please read the notes on the back before filling this page) Order: This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) A7 1243027 V. Description of the invention (ς) Contains any combination of pine bark extract, cypress tree fruit extract, grape seed extract and Peter Temple. Detailed description of the invention: Proanthocyanidin is a powerful free radical scavenger. In particular, proanthocyanidin represents A group of astringent fruits and plant polyphenols found in bark. Proanthocyanidins can be extracted from plant material by conventional methods using water, ethanol or acetone / water mixture as solvent, and then evaporated by solvent , Freeze drying or spray drying. Proanthocyanidins include proanthocyanidins and procyanidins. The proanthocyanidin used in the following examples is Pycnogenolf pine bark extract, manufactured and sold by Horphage Research Co., Ltd. Pycnogenol® pine bark extract is a bark derivative of French maritime pine. It contains Proanthocyanidins with a content of about 70-75% and other flavonols such as catechins, quercetin and phenolic acids with free radical scavenging activity. The proanthocyanidins contained in this extract have a chain length of about 2 to The monomer unit of 12, wherein their monomer units are composed of catechins or epicatechins. Other proanthocyanidin-rich substances can also be used as free radical scavengers in cigarette filters. These substances include, But it is not limited to extracts from pine bark, cypress capsules, or grape seeds. Since proanthocyanidin is a non-volatile substance, it is particularly suitable for cigarette filters. Proanthocyanidin is a kind of pigment that has great continuous absorption and remains in the filter Internally oriented biopolymer. The free radical scavenging filter of the present invention can uniformly and completely spray the free radical scavenger solution on the single fiber of the filter, and then dry the single fiber of the filter 7 paper size In use National Standards (CNS) A4 size (210 x 297 1 complex public issues (read the note and then fill in the back of this page)

1243027 V. Description of the invention () It is made by connecting the filter element to the cut cigarette without cigarette and / or cigarette tobacco used to form the cigarette. Before drying, the filter element can be formed in the filter beam forming process. A variety of specific examples of free radical solutions can be used. The examples and results are discussed below. Example 1 The proanthocyanidin and vitamin C (100%) were dissolved in a 95% ethanol solution at a ratio of 1: 2. Spray the acetamidine solution containing dissolved proanthocyanidin and vitamin C evenly on the single fiber of the cigarette. The sprayed single fiber is then dried, and the dried single fiber is processed into a cigarette filter by a method known in the art. Combine it with cigarettes without filters. The content of proanthocyanidin and vitamin C in the cigarette filter in this example is equivalent to about 0.00015% and 0.003% of the weight of the cut tobacco in this cigarette. The performance test of the improved filter was performed by the following method. Use a cigarette without a filter as a reference cigarette. ESR technology was used to test the gas-phase free radicals contained in each cigarette smoke. The free radical content in the filter of the present invention is compared with that of a standard cigarette without a filter. The effectiveness test of the modified cigarettes was performed by using a smoking device to simulate human smoking at a flow rate of about 400 ml / min, smoking for two minutes at a time, and one minute at a time. ESR test conditions include: X-band, 20 mW microwave power, 100 kHz frequency modulation, and 1 G amplitude modulation. The test results are shown in Table 1. Calculate the radical elimination rate by the following formula: Ε = Η〇X 100 / ΗΧ 8 This paper size is applicable to China National Standard (CNS) A4 (210 χ 297 public love) (Please read the precautions on the back before filling in this page)

A7 1243027 B7. «—- 'V. Description of the invention (y) Wherein, H 0 represents the peak intensity of the control system, and Hx represents the peak intensity of the sample containing the scavenger. According to this formula, the removal rate E of gas phase radicals is 24.3%. Example 2 Using the method of Example 1, according to the steps explained above, a cigarette with an improved filter having a proanthocyanidin content of about 0.00015% (based on the weight of cut tobacco with a single filter) was tested using the above-mentioned free radicals. The division rate formula is calculated. The elimination rate of gas-phase radicals is 22 · 6% °. See Table 2 for detailed results. Example 3 Using the method of Example 1, test the proanthocyanidin content of about 0.003% (based on the weight of cut tobacco with a single filter) of a cigarette with an improved mouthpiece according to the steps explained above and use the above freedom The basis rate formula is calculated. The gas-phase radical scavenging rate calculated from the above-mentioned radical scavenging rate formula is 27.67. . See Table 3 for detailed results. Example 4 Using the method of Example 1, according to the steps explained above, a cigarette with an improved reduced mouth content of about 0.0005% (based on the weight of cut tobacco with a single filter) of proanthocyanidin was tested and the above free radicals were used. The division rate formula is calculated. The gas-phase radical scavenging rate calculated from the above radical scavenging rate formula is 29 · 1% °. See Table 4 for detailed results. This test indicates that when the filter 9 (please read the precautions on the back before filling this page) _ line t (CNS) A4 (210 x 297 ^) ^ ~ " A7 1243027 ________ V. Description of the invention (S) (Please (Read the precautions on the reverse side and fill in this page first) When the proanthocyanidin content is 0.0005%, the gas phase radical removal rate reaches the maximum. Example 5 Using the method of Example 1, test the proanthocyanidin according to the steps explained above. Cigarettes with modified filters containing about 0.001% (based on the weight of cut tobacco with a single filter) are calculated using the above formula for free radical scavenging rate. Phase radical scavenging rate is about 20%. For detailed results, see Table 5. As shown in the example above, when the proanthocyanidin content in the filter is between 0.00015% and 0.001% (the weight of cut tobacco with a single filter) When it is within the range, it can achieve a high rate of elimination of gas-phase free radicals in smoke. Adding vitamin C to the filter further increases the effect of eliminating free radicals. The reduction of free radicals in tobacco smoke also makes tobacco smoke Reduced mutagenic effects And significantly increased the lifespan of animals exposed to filtered smoke. In one study, mice were exposed to a lethal amount of cigarette smoke in a polypropylene glass box (35.6 X 35 x 20 cm) with one hole at the top of the box for ventilation and another hole at the bottom to introduce the gas phase. 40 mice were randomly divided into 4 groups. The mice in group 1 were from cigarettes with standard filters. Smoke was treated. Groups 2 and 3 mice were treated with smoke from cigarettes containing 0.00015% and 0.0003% milligrams of proanthocyanidin and pine bark extract in the filters. Group 4 mice were used as a control group, and It is not treated with cigarette smoke. At the same time, cigarette smoke is introduced into a box containing 10 mice per group. When the lethal end point is recorded, 10 paper sizes are applicable to China National Standard (CNS) A4 (210 X 297 mm) A7 1243027 — _____B7___ V. Description of the invention (/) Time taken and number of cigarettes. Examine the histopathological changes of dead mice. (Please read the note on the back? Matters before filling out this page) Make all dead Mice were sectioned and histopathologically examined. In the control group (without proanthocyanidin in the cigarette filter), significant congestion and bleeding were observed in 80% of the lung tissue. Vasodilation and small vessel congestion in the kidney were also found. And the liver has slight vasodilation and central vascular congestion. However, there are no visible changes in the heart and spleen. The cigarette filter contains 0.0005% proanthocyanidin pine bark extract, which significantly increases survival time and makes the cigarette The acute toxicity caused by smoke was reduced by 70.5%. When the proanthocyanidins were absent in cigarette filters, mice died after inhaling the smoke of 8 cigarettes, and when the filters contained 0.0005% proanthocyanidin pine bark extract, the The rats died after being exposed to the smoke of 14 cigarettes. According to the above, the appropriate content of the above-mentioned free radical scavenger contained in the filter should be estimated to be 0.0001% _0.01% of the weight of the cut tobacco. Within this range, herbicides are more effective. The ratio between the proanthocyanidin content and the vitamin C content is equivalent to 0.5-1.5: 1.5-2.5, and more preferably 1.0. However, in all the specific examples, L-glutathione and selenium sources selected from the group consisting of L-selenomethionine and L · selenocysteine are substantially or completely excluded from this Invention of cigarette filters other than the contents. 11 This paper size applies Chinese National Standard (CNS) A4 specification (210 X 297 public love) A7 1243027 ____B7__ V. Description of the invention (β) Table 1 The filter combining 0.000 15% proanthocyanidin and 0.0003% Vc for smoke in the smoke Gas phase radical scavenging effect Ho in control group Hx 6.7 18.5 7,6 11.5 4.3 1L4 6.2 5.8 5.6 21.5 7.8 7.7 5.6 10.7 5.8 9.5 5.7 14.2 5.5 10.4 5.7 5.2 4.4 5.9 6.9 21.5 6.0 7.2 5.2 5.5 5.6 5.6 7.0 6.5 7.4 7.2 5.9 4.4 10.5 6.5 7.8 6.4 8.2 5,5 7.0 1.5 6.3 10.4 7.4 6.0 8.0 10.3 6,2 6.7 5.6 7.1 10.0 6.0 9.0 11.0 9.0 6.0 5.5 10.7 8.5 6.7 6.2 12.5 6.7 6.0 5.5 7.3 5.7 6.0 6.2 9.5 5.0 6.7 5.7 9.8 6.7 7.4 6.0 12.6 6.3 5.6 9.0 7.0 6.8 7.8 9.2 9.4 5.2 7.0 10.0 8.0 7.4 8.0-.9.5 8.7 7.4 6.0 8.0 6.0 8.0 16.0 8.0 9.8 4.6 5.5 6.8 8.5 7.1 16.0 5.3 6.8 5.0 6.5 7.5 7.2 6.6 17.0 7.3 9.0 5.2 11.8 7.0 6.8 8.9 11.8 8.3 9.6 8.3 11.8 9.1 7.5 9.0 8.0 10.3 8.9 8.2 6,0 6.4 7.0 11.5 9.0 8.1 8.5 8.0 4.0 6.0 6.1 17.0 6.2 9.0 8.8 10.0 5.0 4.5 6.2 (Please read the notes on the back before filling in this (Page)-Line · 12 This paper size is applicable to China National Standard (CNS) A4 (210 X 297 mm) 1243027 A7 B7 V. Description of the invention (11) 7.8 6.0 7.5 9.7 8,4 6.2 7.0 6.7 6.3 9.0 6.5 9.5 6.6 6.1 8.3 7.0 8.8 9.2 11.4 8.9 8.8 11: 8 98 7.1 12.8 8.7 8.5 9.8 10.5 8,7 6.7 7.2 7,7 8.5 8.7 9.7 7.8 4.3 5.6 6.0 5.7 6.9 7.0 7.8 5.2 5.9 7.0 5.2 7.4 10.0 8.5 9.8 9.0 6.7 5.0 6.2 6.7 6.8 7.4 8.0 5.2 7,4 4.6 6.3 7.1 6.6 8.9 9.0 5.2 8.3 8.2 5.0 11.5 17.0 7.8 10.5 10,0 8.4 4.1 3.5 11.9 12.0 10.8 9.8 7.5 3.5 4.1 4.2 (Please read the notes on the back before filling this page) Average値 Standard error elimination effect p 8.96 2.59 6.73 1.81 24.3% < 0.01-line · 13 This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) 1243027 V. Description of the invention (, /) Combination Effect of 0.00015% proanthocyanidin filter on the elimination of gas-phase free radicals in smoke in control group Ho Operation Example 2 χ 4.1 4.6 4.5 4.5 2.0 3.0 4.3 5.1 7.5 4.8 8.0 5.0 4.5 7.5 4.7 4.0 4.0 8.0 13.5 8.0 5.0 3.5 9.0 3.5 6.9 6.2 4.7 5.6.6 7. 8 7.9 7.4 5.1 5.7 6.9 7.0 7.8 5.0 3.9 4.9 5.7 5.5M.9.8.0.6J.5J.00.50.0 678797668811 -ο ο. Ο 8 * 6 J 7 5 J 5 ο LJ ot 1 6 6 1 7 8 7 5 9 9 1 '9 〇6 7.46J7.111.56.66.37.05.58.5U S9.210.5 .7.0ο ο 8 5.069.7.06 ο · ο 5 i od ο 9 ο 1 & 7. · 6.3.2l · J.0.01056 6 6 6 6 41vo7vci4.4.4.76. Ο JAJ 4 ο 6 ο ο ο ί ο 7 6 6 1 6 65.4.4.6.7. · 6. .7.0-.9.0.20 6 ο ο 5 5 7 ο 6 7 6 7 6 06 7 · 5.5.5.5.5.1 JJ o. J1.57 J 5 ο 0 3 8 ο 5 7 5 6 1 7 4 74.4.7.4.8. Ο & (Please read the notes on the back before filling (This page)-Line-This paper size is in accordance with Chinese National Standard (CNS) A4 (210 X 297 mm) A7 1243027 V. Description of the invention (θ) Poor results and errors are excluded except for the flat standard 淸 5.97 1.90 22.6% < 0.05 (Please read the notes on the back before filling in this page) 15 This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) 1243027 A7__V. Description of the invention (4) Table 3 combined with 0.0003 % Proanthocyanidin filter for the elimination of gas-phase free radicals in smoke Control group 18.5 6.5 18.5 6.8 16.5 5.3 1 5.5 5.9 15.2 5.8 15.0 7.7 15.0 5.5 .13.7 5.4 13.3 5.8 13.0 7.8 12.0 6.2 1L2 7.9 10.0 6.0 8.0 6.5 9.0 6.0 7.8 ·· 7.1 6.7 6.1 18.5 5.5 6.5 6.4 6.7 6.0 H〇9.9 5.2 7.3 5.8 7.5 7.2 7.5 9.0 7.0 8.8 6.1 8.5 6.5 7.4 8.0 10.5 6.6 8.0 7.0 6.6 9.0 6.5 8.6 5.7 6.0 7.2 6.5 7.3 .5.0 7.8 6.8 7.0 5.9 7.4 14.2 5.5 6.0 6.0 7.4 7.8 Operation example 110 6.7 12.0 5.6 U.0 6.1 10.3 5.7 10.0 6.7 10.0 7.0 9.9 7.1 9.5 7,8 9.0 7.8 8.2 5.1 8.0 7.1 8.0 5.1 7.0 5.6 6.5 6.8 7.2 4.2 6.0 8.0 7.1 5.3 10.5 11.2 3.6 8.4 4.0 5.5 Hx 5.3 4.4 6.0 7.56.0 5.2 5.4 4.66.0 3.0 4.2 4.5 4.0 3.7 5.4 4.2 6.76. 0 2.7 6.5 4.26.0 6.2 6.1 7.0 7.0 6.1 3.9 6.0 7.2 6.7 3.2 4.1 4.5 10.5 8.0 5.1 4.7 5.7 4,5 (Please read the precautions on the back before filling out this page) Packing. Ί Line · 16 This paper size applies to China National Standard (CNS) A4 Specification (210 X 297 mm) 1243027 A7 B7 V. Invention Description 8.0 16.0 11.8 8.0 6.0 7.6 6.0 7.4 6.0 5.0 6.7 5.6 5.7 6.7 7.8 9.8 5.1 8.2 5.3 8.0 6.5 8.8 5.8 7.7 5.8 7.8 9.2 8.0 9.8 9.8 8.0 16.0 17.0 9.0 9.5 7.8 10.5 8.2 7.9 6.2 9.7 6.0 10.9 7.0 9.8 5.7 8.1 5.6 8.9 7.5 9.0 5.3 9.6 8.5 9.8 6.2 7.9 8.5 9.9 9.5 10.5 12.0 10.5 11.8 5.0 7.7 7.0 6.5 3.5 5.2 6.0 6.9 5.6 3.7 6J 2.0 2.2 3. »4.6 4.3 2.5 5.2 5,4 3.0 4.2 5.2 3.7 2.7 6.5 6.5 6.1 10.5 6.0 5.2 5.0 6.0 6.0 8.0 2.3 2.7 6, 2 2.9 2.6 4.6 .4.5 5.4 4.2 2.0 5.0 • 4.0 9.0 5.0 5.0 8.2 6.8 5.0 6.0 5.2 4.4 5.0 4.5 (Please read the precautions on the back before (Fill in this page)

Average 値 standard error removal effect P 8.302.92 6.012.1227.6% < 0.01 17 This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) 1243027 A7 B7 V. Description of the invention (A) Table 4 Effect of 0.0005% proanthocyanidin filter on the elimination of gas-phase free radicals in smoke in the control group Ho in operation example 4 Hx 7.9 15.0 5.8 6.7 5.4 2.0 6.2 6.5 8.7 18.0 5.9 6.0 5.8 10.5 7.0 6.8 9.7 15.0 6.2 7.4 4.9 11.0 6.2 7.0 7.0 19.0 6.1 7.8 7.0 6.6 5.0 7,0 8.6 16.5 5.0 8.0 8.0 10,3 3.5 3.9 8.8 7.3 6.3 16.0 8.0 7.0 6,6 2.5 · 9.4 8.0 5.2 16.0 8.7 6.0 4.1 8.5 10.1 110 7.1 17.0 6.7 8.6 2.6 4.1 7.0 1L2 7.5 11.8 8.7 9.6 2.6 4.8 7.5 13.0 7.6 8.0 6.5 5.8 1.2 5.2 8.7 13.3 6.5 9.0 5,6 1.8 1.9 5.5 9.6 11.2 6.9 6.2 6.7 11.0 5.9 5.0 6.1 18 · 1_ 6.8 '6.0 7.6 10.7 4.6 6.1 5.9 15.2 5.9 7.6 5.5 9.8 4.0 10.0 6.6 15.5 62 7.8 5.5 9.7 4.7 7.4 6.2 10.0 18.5 5,5 6.0 10.0 5.4 10.0 6.3 13.7 23.7 6.0 5.0 9.0 3.0 8.0 7.4 7.2 14.2 7.4 6.7 6,7 6.2 5.0 9.1 6.2 6.5 8.2 6.6 5.0 6.4 8.0 6.4 6.0 6.6 6.0 7.1 5.8 5.6 9.8 (Please read the precautions on the back before filling in this page) Installation-Line-This paper size is applicable to Chinese National Standard (CNS) A4 (210 X 297 mm) 1243027 A7 B7 Instructions (\ 1) 5.0 6.2 6.2 6.9 3.8 3.0 4.4 4.5 9.2 9.5 6.0 8.2 5.7 5.8 57 8.5 10.3 8.1 9.0 7.5 7.7 9,5 7,5 8.2 7.2 6.2 5.8 5.9 5.0 62 62 7.0 • 6.2 8.2 8.1 5.0 8.3 5.0 3.5 6.6 4.1 5.3 7.7 7.5 7.6 2.6 2.6 1.2 1.9 8.5 8.5 8.9 6.8 4,7 V 5.9 4.6 4.0 4.7 5.9 6.2 7.9 8.0 5.4 5.4 3.0 5.4 5.8 9.7 7.0 8.6 8.0 4.9 7.0 6.0 6.0 8. $ 8.2 10.1 7.0 62 6. · 6.7 6.7 7.5 8.7 9.6 6.1 6.5 5.6 6.7 7.6 5.9 8.6 6.2 6.3 5.5 5.5 6.0 5.0 7.4 9.1 6.7 6.6 -------------- Install --- (Please read the precautions on the back before filling this page).

Average 値 standard error elimination effect P 8.62 3.39 6.11 2.17 29.1% < 0.01 -line-19 This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) 1243027 A7 _B7_ V. Description of the invention (1 Each) Table 5 The effect of removing 0.001% proanthocyanidin filters on gas-phase free radicals in smoke in the control group Ho Operation Example 5 Η 6.6 8.5 7.8 6.6 1.2 6.5 5.8 12 6.6 6.0 8.0 5.6 5.8 6.0 11.1 5.9 8.6 5.4 16.0 8.6 4.0 4.8 12.0 4.0 6.9 6.1 16.0 5.9 4.9 12 1L8 4.9 5.8 6.1 17.0 5.3 5.2 6.2 .11.0 5.2 6.4 7.8 11.8 6.4 4.5 6.6 12.5 4.5 7.1 7.8 8 · 5.1 5.1 8.0 5.7 9.0 6.0 8.2 5.7 9.0 8.2 6.2 4.7 6.7 6.2 6.3 6.0 6.2 6.3 5.9 5.0 5.2 5.9 6.7 8.5 6.0 8.1 5.2 6.0 7.0 5.2 5.7 8.0 7.6 5.2 5.2 6.0 7.0 5.3 6.9 53 7.8 6.9 5.1 6.3 6.5 5.1 6.2 5.8 5.5 6.2 5.1 2.7 4.0 5.1 7.8 7.1 6.0 8.8 6.0 6.0 8.0 6.0 6.8 7.2 — 7.4 8.8 4.0 6.9 5.0 4.0 5.8 5.9 8.2 8.6 5.6 2.9 5.4 5.6 6.7 6.5 6.0 8.1 4.1 7.0 5.5 4.1 5.7 8.1 5.0 5.2 4.7 5.8 5.0 4.7 5.6 5.0 6.2 5.0 5.0 6.0 4.3 4.3 5.2 5.9 6.5 6.2 6.7 5.2 4.3 5.0 5.2 5. 9 6.0 6.0 5.4 5.6 5.7 9.5 5.1 7.2 6.5 9.2 5.7 4.3 5.0 10.7 5.1 20 This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) -------------- -(Please read the notes on the back before filling this page) -Line A7 1243027 __B7 V. Description of Invention (丨 /) 7.5 6.7 9.5 5.6 4.9 6.6 9.5 4.4 6.5 18.5 6.0 5.9 4.9 12.5 7.5 5.0 6.7 21.5 5.3 7.2 6, 1 10,7 8.2 5.2 7.3 14.2 7.3 7.5 5.8 11.5 8,0 .5.6 7.6 21,5 8.2 6.5 .7.7 8.7 6.0 4.3 5.8 6.5 10.3 6.7 6.1 4.8 6.4 4.9 6.4 6.4 8.1 7.3 5.6 2,0 4.9 6.0 6.6 6.0 9.0 7.6 4.9 6.0 5.8 5.6 6.3 6.0 7.4 7.8 6.5 5.6 7.1 6.1 7.3 6.7 6.7 7.8 7.1, 4.0 5.6 4.9 7.8 6.0 6.0 8.5 7.1 6.0 3.5 6.0 Mean 値 standard error elimination effect P 7.45 2.79 5.96 2.02 20.0% < 0.05 ---- ------------- (Please read the notes on the back before filling out this page) Line-21 This paper size applies to China National Standard (CNS) A4 (210 X 297 mm)

Claims (1)

1243027 A8B8C8DS 6. Application scope and (C) Filter element of drying step (b). 8. The method according to claim 6 of the application, further comprising the step of attaching the filter element to the cigarette. 9. The method of claim 8 further comprising the step of combining the filter element with other parts of the cigarette. 10. The method according to item 7 of the application, wherein the dissolving comprises dissolving vitamin C in the solution. The method according to item 5 or 6 of the patent application scope, wherein the filtering component includes vitamin C. 12. The method according to item 11 of the application, wherein the ratio of the content of the filtering component to the content of vitamin C is 0.5-1.5: 1.5-2.5. 2 • ...................... Fee-i! (Please read the notes on the back before filling out this page) The paper size of this edition applies Chinese national standards ( CNS) A4 specification (210 X 297 mm) 1243027 Date of application? /, 4 Case No. 〇1 1/1 > V 6 ^ Category D The above columns are filled by the Office) cloud! Patent specification Chinese invention new name English name Nationality Cigarette mouthpiece with scavenging effect on free radicals in cigarette smoke and preparation method thereof A CIGARETTE FILTER WITH SCAVENGING EFFECT OF FREE RADICALS IN CIGARET-TE-SMOKE ANDITS-PR-EPARATION METH € D People's Livelihood and Residence of the Invention ⑴ Zhao Baolu (2) Peter Widward ⑶ Li Jingnong ⑴ Mainland China ⑵ Germany (3) Mainland China (1) 15 Datun Road, Chaoyang District, Beijing 100101, China (2) Münster, Germany 48161 Twente Road ⑶ Beijing, China 10016 Chaoyang District West Road RM816 Tower Crown Plaza Maizi Store 3 Binding Name (Name) Harper Research (Lusenbao) Holding Company Line Nationality Luxembourg Applicant Residence, Residence (Office) Name of the representative, No. 14A Bans Road, L-1212, Luxembourg. The paper size is applicable to the Chinese National Standard (CNS) A4 specification (210 × 297 mm) 1243027 A8B8C3D8 Φ ΟΜΙ 6. The scope of patent application L is a cigarette filter, It includes a filter element containing proanthocyanidin as a percolation component, and the content of the filter component The amount is an amount effective to scavenge gas-phase free radicals present in cigarette smoke without significantly changing the flavor and mouthfeel of the cigarette, and the filter is substantially free of L-glutathione. 2. [[Cigarette filter of item 1 of the scope of patent application, which is combined with the other parts of the cigarette, and the content of the filtering component is 0.0001% to Between 0.001%. 3- The cigarette filter of claim 1 or 2, wherein the filter element further comprises vitamin C. 4. The cigarette filter according to item 3 of the patent application, wherein the ratio of the content of the filtering component to the content of vitamin C is 0.5-1.5: 1.5-2.5. 5. A method for manufacturing a cigarette filter, comprising providing a filter element containing proanthocyanidin as a filtering component, the content of the filtering component is effective to remove gas-phase free radicals present in cigarette smoke without simultaneously The amount of flavor and mouthfeel of the cigarette is significantly changed, and wherein the filter is substantially free of L-glutathione. 6. If the method of claim 5 is applied in combination with other parts of the cigarette, the content of the filtering component is between 0.0001% and 0.001% of the weight of the cut tobacco contained in the other parts of the cigarette . 7. The method of claim 5 or 6, wherein the providing includes: (a) dissolving a defined amount of a filtering component in an approximately 95% ethanol solution; (b) substantially substituting the solution of step (a) Spray evenly on the filter element; 1 _ This paper size applies to China National Standard (CNS) A4 specification (210 X 297 public love) ----------------- ------..... (Please read the notes on the back before writing this page), 1Τ: