KR20090069609A - Bed-soil composition comprising bacillus sp. microbe and preparation method thereof - Google Patents

Abstract

A bed soil composition containing microorganisms belonging to bacillus and a preparation method thereof are provided to promote the rearing of crop seedling such as lettuce and cucumber, thereby raising seedling in organic farming cultivation of leafy vegetables and fruit vegetables. A bed soil composition containing microorganisms belonging to bacillus comprises a solid part and a liquid part. The solid part contains 35-55vol% of cocopeat, 25-45vol% of peat moss, 8-12vol% of vermiculite and 8-12vol% of perlite. The liquid part contains liquid fertilizer and bacillus spp SL9-9 strain(KACC 91232P) culture material showing cellulase activity. The liquid fertilizer represents a mixed fermented liquid containing mackerels, molasses and bacillus spp SL9-9 strain. The content of the liquid fertilizer is 0.1-3vol% on a volume basis of the solid part. The density of bacillus spp SL9-9 strain culture material is 105~107cfu/ml. The content of bacillus spp SL9-9 strain is 0.1-5vol% on a total volume of the solid part.

Description

Bacillus microorganism-containing clay composition and a method for producing the same {BED-SOIL COMPOSITION COMPRISING BACILLUS SP. MICROBE AND PREPARATION METHOD THEREOF}

The present invention relates to a topsoil composition containing microorganisms of the genus Bacillus and a method for preparing the same, and more particularly, to a solid part containing cocopipit, pitmos, vermiculite and pearlite, and SL9-9 strains of the genus Bacillus exhibiting liquid and cellulase activity (Accession No. : KACC 91232P) The present invention relates to a bacterium-containing microorganism-containing clay composition comprising a mixture of liquid portions containing a culture, and a method for preparing the same.

Seeds germinate during crop cultivation, and the young roots that have just penetrated the seedlings touch the soil for the first time. The role of the soil is the place where the roots of young plants grow, which increases the germination rate of seeds, mechanically supports the plants, and the roots. It is the most basic and important hair raising material that is responsible for the growth of various cultivated plants, such as appropriately formulating growth conditions (temperature, moisture, oxygen, etc.) and supplying various effective nutrients.

Therefore, depending on the soil, the plant may have problems such as nutrient imbalance and excess nitrogen, pest infection, seed germination and growth inhibition, growth unevenness and weed seed generation. Soil's importance is increasing day by day, since it is possible to grow healthy seedlings through artificial topsoil control before transplanting.

Initially, the soil was mixed with soil and compost to make a mixed soil for 6 months to 1 year, and it was generally used, but the physical and chemical properties of the soil were changed according to the material and season, resulting in uneven growth of crops. . However, in recent years, the development of agriculture-related industries, the development of seedling technology, lighter and more stable, the balance of nutrients necessary for growing crops, and the specialized soil has been produced and supplied.

In general, the materials of the topsoil include peat moss, coco peat, rice hulls, bark, side lobe, and huntan as organic substances, and pearlite, vermiculite, zeolite, diatomaceous earth, bentonite and the like are used as inorganic substances, and two or more of them are used. The mixture is mixed with the additives such as lime, a bioactive agent, an acid and a hygroscopic agent, and manufactured in the soil.

Recently, due to growing interest in environmental pollution and opening up of agricultural imports, synthetic organic fertilizers and pesticides are excluded to be applicable to the production of eco-friendly (organic) agricultural products as eco-friendly organic methods have been expanded for safer food production and improved soil environment. Production of the topsoil is required. In particular, research on integrating environmentally friendly organic methods with microorganisms that can promote the growth of crops or supply nutrients among indigenous rhizosphere microorganisms living in the root zone of existing crops is needed.

Accordingly, the present inventors have isolated and selected indigenous microorganisms of Bacillus, which have high cellulase activity, and added them to the soil for use in organic cultivation to confirm that the bacterium containing Bacillus microorganisms had an effect of promoting germination and seedling growth of crops. By this, the present invention was completed.

Accordingly, the present invention is a genus of Bacillus comprising a mixture of liquid phase containing a cultured cocoa, peat moss, vermiculite and perlite and a Bacillus SL9-9 strain (Accession No .: KACC 91232P) culture exhibiting liquid fertilization and cellulase activity. An object of the present invention is to provide a microorganism-containing clay composition and a method for producing the same.

Bacillus sp. Microorganism-containing soil of the present invention contains Cocopit, peat moss, vermiculite and perlite in a ratio suitable for crop germination and growth, and also contains Bacillus sp. SL9-9 strain having cellulase activity and organic substance maturation effect. (Accession No .: KACC 91232P) and the fermentation broth of natural organisms and SL9-9 strains of Bacillus (Accession No .: KACC 91232P) to promote the germination and seedling growth of crops such as lettuce and cucumber By showing, it can be usefully used as leafy vegetables and fruit vegetables organically grown seedlings.

The present invention is a genus of Bacillus microorganism comprising a solid phase containing cocofit, peat moss, vermiculite and pearlite, and a liquid portion containing Bacillus SL9-9 strain (Accession No .: KACC 91232P) culture showing liquid fertilizer and cellulase activity. It contains a containing topsoil composition.

Hereinafter, the present invention will be described in detail.

The present invention includes a microorganism of the genus Bacillus comprising a solid phase containing cocopite, peat moss, vermiculite and pearlite and a liquid portion containing a culture of Bacillus SL9-9 strain (Accession No .: KACC 91232P) exhibiting fertilizer and cellulase activity. The present invention relates to a top soil composition and a method for manufacturing the same, wherein the Bacillus SL9-9 strain having cellulase activity is contained in a Bacillus microorganism-containing top soil composition to promote the growth of crop seedlings such as lettuce, cucumber, and the like. The present invention relates to a Bacillus microorganism-containing clay composition and a method for preparing the same, which may be usefully used for organically grown hair.

Cocopit contained in the present invention (cocopeat) is a sieve filtering the by-products generated while extracting the palm fiber from the coconut, generally containing 3 to 10% by volume of the palm fiber of 3 to 15 mm in length.

In addition, the peat moss contained in the present invention is widely used as an organic material of the top soil, and is widely used as an excellent soil improving agent because it has excellent drainage, aeration, bobbin, and water retention, so that it is suitable for crop cultivation. It can be mixed with various mineral materials (vermiculite, pearlite, lime, fertilizer, humectant, etc.) and organic materials to make various soils suitable for growing various crops, breeding and potting soils such as vegetable seed sowing, various flower planting and cutting. As good as.

Further, vermiculite contained in the present invention is a clay mineral made of hydroxide silicate of aluminum, magnesium and iron, which is easily decomposed into acid and has a high cation exchange capacity. It expands when heated. It is porous and has good absorption ability, and is widely used as a heat-resistant material and a soundproofing material.

In addition, the perlite (perlite) contained in the present invention is a pumice that has been added to the pores by sharply adding pearlite, and is a synthetic soil having characteristics of promoting early slidability of crops due to good breathability and water retention. It has no base replacement capacity and has no binding power, but it is used as soil improver to increase soil pore and as a supplementary material for crop or horticultural soil.

The four components contained in the solid portion of the bacterium-containing microorganism-containing clay composition of the present invention contain 35 to 45% by volume of Cocopit, 35 to 45% by volume of vermiculite, 8 to 12% by weight of vermiculite, and 8 to 12% by weight of pearlite, respectively. It is characterized by.

If the cocofit is more than 35 to 45% by volume, the content of peat moss is relatively reduced, and there is a disadvantage in that the germination rate of the crop and growth of the nanny are sluggish, and when the content is less than 35 to 45% by volume, the relatively acidic peatmoss It is not preferable because the amount of addition is relatively high, which causes a disadvantage that affects the pH of the soil.

In addition, when the peat moss is contained in more than or less than 35 to 45% by volume, it affects not only the chemical properties such as the pH of the soil but also the physical properties, and the disadvantage of poor seedling growth is not preferable.

In addition, when the vermiculite and perlite are contained in an amount of more than 8 to 12% by volume or less, this may affect the chemistry and physical properties of the soil and also cause the disadvantage of poor seedling growth.

The volume ratio of cocoite: pitmos: vermiculite: pearlite in the topsoil composition of the present invention is most preferred because it is excellent in germination rate and growth of seedlings.

In addition, the liquid ratio contained in the liquid portion of the clay composition of the present invention is characterized in that the mixed fermentation broth of the mackerel, molasses and Bacillus SL9-9 strain.

Liquid fertilizer is generally a mixture of organic matter, nitrogen, phosphoric acid, kali, minerals, water, and the like, and is added to the soil as a nutrient source and serves to supply nutrients to plants during crop growth. Bacillus microorganisms containing the microorganism of the present invention is for organic seedling, so the liquid ratio contained therein is not a mixture of artificial chemicals, but a fermentation by adding microorganisms to marine by-products such as wild mackerel (breast, fish, early, dome, etc.) In particular, it is characterized by showing synergistic effect in terms of growth effects by fermentation by addition of the strain SL9-9 of the genus Bacillus (Accession Number: KACC 91232P) as a microorganism. In particular, the Bacillus SL9-9 strain exhibits Hemicellulase, Protease and Lipase activity as well as Cellulase activity, which promotes protein breakdown and lipolysis of fish, resulting in no odor during fermentation of liquid fertilizers, and low residue after fermentation. .

According to a preferred embodiment of the present invention, the liquid ratio of 40 liters of raw mackerel 40 liters of the Bacillus SL9-9 strain active liquid (mixture prepared by mixing 40 liters of water, 10 liters of molasses and 2 liters of Bacillus SL9-9 strain) It is prepared by fermentation.

The liquid ratio is preferably contained in a volume ratio of 0.5 to 3 with respect to the total solids volume 100, more preferably in a 1-2 volume ratio relative to the total solids volume 100. When the liquid ratio is added in excess of 3 volume ratios with respect to the total solid volume 100, the growth of the nanny rapidly deteriorates (Table 4).

In addition, the Bacillus SL9-9 strain (Accession Number: KACC 91232P) containing the liquid portion in the soil composition of the present invention has high cellulase enzyme activity, and is contained in the soil of the present invention, and exhibits an organic material ripening and crop growth promoting effect.

The Bacillus genus SL9-9 strain culture is preferably 10 ⁵ ~ 10 ⁷ cfu / ml. The Bacillus genus SL9-9 strain is preferably contained in a volume ratio of 0.1 to 5 with respect to the total volume of the solid phase, and more preferably in a volume ratio of 1 to 2 with respect to the total volume of the solid phase.

In particular, when the density of the SL9-9 strain culture contained in the Bacillus microorganism-containing soil of the present invention is about 10 ⁷ cfu / ml, it is contained in a volume ratio of 1 to 2 relative to the total volume of the upper portion of the upper layer in the promotion of crop growth desirable.

The present invention also comprises the first step of adding a suitable amount of water to the mixture of coco-pit 35-45% by volume and 35-45% by volume peatmoss first mixing; A second step of secondly mixing 8-12% by volume of vermiculite into the cocoite and pitmos mixture of the first step; A third step of adding and mixing the liquid ratio to the mixture of the two steps; A fourth step of preparing a SL9-9 strain (Accession Number: KACC 91232P) culture of Bacillus; A fifth step of adding the Bacillus genus SL9-9 strain culture to perlite in step 4; Provided is a method for producing microorganisms containing Bacillus genus soil comprising a sixth step of mixing the mixture of the three stages of pearlite of 8 to 12% by volume to the mixture of the three stages.

The first step is the first step of adding water to the coco peat and peat moss mixture contained in the solid part of the Bacillus microorganism containing soil of the present invention, the first mixing, 35 to 45% by volume of coco peat and 35 to 45% by volume of peat moss Mix and then add and mix the appropriate amount of water.

In the second step, vermiculite is added to the first mixture of the first step, followed by secondary mixing. The vermiculite is added by adding 8 to 12% by volume of vermiculite to the entire mixture.

The third step is to add a nutrient source by adding and mixing the liquid ratio in a volume ratio of 0.1 to 3% with respect to the volume 100 of the cocoite, pitmos and vermiculite mixture obtained in step 2.

The fourth step is to prepare by culturing the Bacillus SL9-9 strain (Accession Number: KACC 91232P) contained in the liquid part of the Bacillus microorganism-containing soil of the present invention,

Seed culture conditions were inoculated with colonies of the strain SL9-9 of Bacillus in a basic medium added to rice bran and incubated at 28 ℃ for about 6-7 days, about 150 rpm and then inoculated. In addition, 5L fermenter containing 3L rice bran added_5L Fermenter was inoculated again and incubated under the same conditions as seed culture conditions and incubated to have a density of about 1 × 10 ⁵ to 10 ⁷ cfu / ml.

Step 5 is a step of adding the Bacillus genus SL9-9 strain culture of the fourth step to the pearlite, the Bacillus genus SL9-9 strain culture for the solid phase (cocoot, peatmos, vermiculite and pearlite mixture) volume 100 It is characterized in that it is added in a volume ratio of 1 to 5.

In particular, the Bacillus SL9-9 strain culture is first added to the perlite, the perlite is characterized in that the activity is high and stable after the addition of the strain among the various biocarriers.

The sixth step is to add and mix 5 to 8% by volume of the pearlite of step 5 to the mixture of the three steps (cocopit, peat moss and vermiculite mixture) to finally complete the production of microorganisms containing Bacillus genus of the present invention.

The liquid fertilizer of the present invention is a mixture of fermentation by adding microorganisms to fishery by-products such as mackerel and other molasses, such as mackerel, molasses, and molasses, and in particular, as a microorganism, the strain SL9-9 (Accession No .: KACC 91232P) was added to fermentation.

According to a preferred embodiment of the present invention, the liquid ratio of 40 liters of raw mackerel 40 liters of the Bacillus SL9-9 strain active liquid (mixture prepared by mixing 40 liters of water, 10 liters of molasses and 2 liters of Bacillus SL9-9 strain) It is prepared by fermentation.

The liquid ratio is added and mixed at a volume ratio of 0.5 to 3 with respect to 100 volumes of cocopit, peat moss, vermiculite and pearlite mixture obtained in step 5 to finally prepare the bacterium-containing microorganism-containing soil of the present invention.

Thus prepared Bacillus microorganism-containing microorganism of the present invention, Cocopit, Pitmos, vermiculite and perlite are mixed in the optimum ratio for crop germination and growth, and also Bacillus genus SL9-9 which has cellulase activity and organic matter ripening effect It contains a strain (Accession No .: KACC 91232P) and contains fertilizer, a fermentation broth of natural organisms and SL9-9 strains of Bacillus (Accession No .: KACC 91232P), which has the effect of promoting the growth of seedlings of lettuce and cucumbers. As a result, it can be usefully used as leafy vegetables and fruit vegetables for organically grown hair.

Hereinafter, preferred examples are provided to aid in understanding the present invention. However, the following examples are merely provided to more easily understand the present invention, and the contents of the present invention are not limited by the examples.

Example

Example 1 Preparation of Bacillus sp. Microorganism-containing clay of the present invention

Bacillus microorganism-containing soil of the present invention for optimal seedling growth was prepared as follows.

40 liters of cocopite and 40 liters of peat moss were mixed and 12 liters of water was added thereto. They were evenly mixed and 10 liters of vermiculite were added.

A liquid ratio was added to 1-2 volume% with respect to volume 100 of a solid part (coco peat, pitmos, vermiculite, and pearlite mixture), and it mixed here.

Two liters of the Bacillus SL9-9 strain (Accession Number: KACC 91232P) culture solution (approximately 1 × 10 ⁷ cfu / ml) were added, and 10 liters of the pearlite mixed therein was added, mixed, and packaged.

Example 2 Selection of Mixture Conditions Containing Microorganisms of Bacillus Species of the Present Invention

For the production of microorganisms containing Bacillus sp. Microorganisms of the present invention, the optimum conditions of the solid phase and liquid phase combination of the raw material was selected.

① Selection of solid phase compounding condition

Cocopit, peat moss, perlite and vermiculite contained in the solid phase were blended according to Table 1, and seedling growth after seeding of lettuce and cucumber was investigated to select the best blending condition. No fertilizers and strains were added here. And the results are shown in Table 2 and Table 3.

(T4 treatment was performed to compare with T5 treatment)

As a result of cultivation of lettuce and cucumber according to the blending conditions, the top part of the top soil showed the best germination rate and growth of lettuce and cucumber when the cocoot, peat moss, pearlite and vermiculite were 40%, 40%, 10% and 10% (vol%), respectively. Good (Tables 2 and 3).

(The germination rate and growth results of Tables 2 and 3 are obtained by using only the solid part of various mixing conditions)

② Selection of Mixing Conditions for Liquid Part

A liquid fertilizer for the addition of soil was prepared for the nutrient supply of the soil, and the selection of the optimum concentration of the liquid fertilizer and strain addition as a nutrient source was performed as follows.

First, the liquid ratio for the top soil addition of the present invention was prepared as follows.

Soil developed in the present invention is intended to utilize for organic seedling

Raw materials for liquid fertilizer was also used instead of chemical mackerel to be suitable for organic certification, and the liquid fertilizer was prepared by adding SL9-9 strain (Accession Number: KACC 91232P) in Bacillus.

That is, liquid fermentation was made by adding 40 kg of raw mackerel and 40 L of microbial active solution to fermentation at room temperature for at least 10 months, _ after fermentation was completed, the liquid fertilization was prepared.

The microbial activator was prepared by adding 10 L molasses and 2 L (5%) of microbial probiotics to 40 L of tap water, and the microbial probiotics were inoculated with a Bacillus SL9-9 strain in _ rice bran 1% addition base liquid medium, Incubated at 28 ℃ 6-7 days to prepare about 1 × 10 ⁷ cfu / ml. This microbial progeny was also used as a strain added to the soil below.

To select nutritional fertilizer and strain concentration in lettuce seedling soil, the liquid fertilizer (nutrient) concentration was 0, 0.5, 1, 2, 3 and 4% (v / v), and SL9-9 strain of Bacillus genus (1). × 10 ⁷ cfu / ml) concentration of 1% (v / v) and 5% (v / v), the growth of lettuce seedlings under various treatment conditions were confirmed.

Table 4 above shows the germination rate of day 7 of lettuce seedlings.

In addition, the results of observing weekly (stem weight) and root weight (root weight) in lettuce seedling growth by treatment group (FIG. 1), and the results of observing leaf length (leaf length) and root length (root length) in lettuce seedling growth by treatment group. (FIG. 2), the results of observing the leaf area and the chlorophyll content in lettuce seedling growth by treatment group (FIG. 3) and the results of the weekly change of the lettuce seedlings by treatment group were shown (FIG. 4 and FIG. 5).

In addition, in order to select nutrient and strain concentration in cucumber seedling soil, the concentration of liquid ratio (nutrient source) was 0, 1, 2, 3, 4% (v / v), and the concentration of strain addition was 2% (v / v). ), The strain density was treated in the untreated, 1 × 10 ⁵ , 1 × 10 ⁶ , 1 × 10 ⁷ , 4 levels.

Figure 6 is the result of measuring the height and week on the day 33 when growing cucumber seedlings by treatment of Table 5, Figure 7 is the result of measuring the root length and weight on day 33 when growing cucumber seedlings by treatment of Table 5, Figure 8 Is the result of measuring the leaf area and chlorophyll content on day 33 when growing cucumber seedlings.

At the concentration of nutrient source and strain added to the liquid, lettuce is 1% strain (strain density 1 × 10 ⁷ cfu / mL), when 1% nutrient is added, cucumber is 2% strain condition (strain density 1 × 10 ⁷ cfu / ml) The best growth was achieved at 2% of nutrient source.

9 is a photograph observing the growth of cucumber by strain density when 2% of the bacterial culture in the Bacillus microorganism-containing microorganism of the present invention (33 cucumber seedlings), compared to the untreated group of the genus Bacillus SL9-9 strain ( Accession No .: KACC 91232P) The growth of cucumber seedlings can be seen to be excellent when the culture medium is treated.

10 is normal soil (wormwood, Nongwoo Green Tech, peat moss 25-35%, cocopit 40-50%, pearlite 9-13%, vermiculite 8-12%, zeolite 8-13%, water-soluble fertilizer, plant growth regulator And a wetting agent) and a photograph of lettuce growth in the Bacillus microorganism-containing clay developed in the present invention (27 lettuce and 32 lettuce seedlings), when the Bacillus microorganism-containing clay of the present invention is treated as compared to normal soil The growth of lettuce seedlings was excellent.

11 is a photograph showing the growth of cucumber in normal soil, untreated strain without bacteria in the soil of the present invention and soil containing microorganisms of the genus Bacillus of the present invention (cucumber 33 seedlings), untreated strain Cucumber seedling growth was significantly better in the Bacillus microorganism-containing soil of the present invention than the group, and it was observed that cucumber seedling growth was superior to normal soil.

FIG. 1 is a result of observing weekdays and root weights at day 32 in lettuce seedling growth according to treatment groups of Table 4,

2 is a result of observing the leaf length and root at day 32 in lettuce seedling growth according to treatment groups of Table 4,

Figure 3 is the result of observing the leaf area and chlorophyll content on day 32 in lettuce seedling growth according to treatment groups of Table 4.

4 and 5 are the results of measuring the change of the weekly week of the lettuce nanny by treatment section of Table 4.

Figure 6 is the result of measuring the height and weekday on the 33rd day of cucumber seedling growth in each treatment group of Table 5,

7 is a result of measuring the root length and muscle weight at day 33 at the time of growing cucumber seedlings according to treatment groups of Table 5,

Figure 8 is the result of measuring the leaf area and chlorophyll content on day 33 at the time of growing cucumber seedlings according to treatment groups of Table 5.

Figure 9 is a photograph observing the growth of cucumber according to the strain density of the bacterial culture in the Bacillus microorganism-containing soil of the present invention (cucumber 33 days seedling).

10 is a photograph observing the growth of lettuce in the normal soil and the bacterium containing Bacillus microorganism of the present invention (27 days of lettuce and 32 days of lettuce).

11 is a photograph observing the growth of cucumber in normal soil, bacteria-free soil, microorganism-containing soil of the genus Bacillus of the present invention (cucumber 33 seedlings).

Claims (10)

Solid phase containing coco peat, peat moss, vermiculite and pearlite, and A Bacillus sp. Microorganism-containing soil composition comprising a mixture of liquid parts containing a liquid and a Bacillus sp. SL9-9 strain (accession number: KACC 91232P) culture exhibiting fertilizer and cellulase activity. The microorganism-containing microorganism composition according to claim 1, wherein the solid part contains 35 to 55% by volume of cocoite, 25 to 45% by volume of peat moss, 8 to 12% by weight of vermiculite, and 8 to 12% by weight of pearlite. The microorganism-containing microorganism-containing clay composition according to claim 2, wherein the solid phase portion has a volume ratio of cocopit: pitmos: vermiculite: pearlite in a 4: 4: 1: 1 ratio. According to claim 1, wherein the liquid fertilizer Bacillus microorganism-containing clay composition is a mixed fermentation broth of the mackerel, molasses and Bacillus SL9-9 strain. According to claim 4, wherein the liquid ratio is Bacillus microorganism-containing clay composition is contained in a volume ratio of 0.1 to 3 with respect to the total solid volume 100. According to claim 1, Bacillus genus microorganism-containing soil composition of the genus SL9-9 strain culture is 10 ⁵ ~ 10 ⁷ cfu / ml. The Bacillus microorganism-containing clay composition according to claim 6, wherein the Bacillus SL9-9 strain is contained in a volume ratio of 0.1 to 5 with respect to 100 of the total volume of the solid phase. A first step of adding the appropriate amount of water to the mixture of coco-pit 35 ~ 55% by volume and 25 ~ 45% by volume of pitmos; A second step of secondly mixing 8-12% by volume of vermiculite into the cocoite and pitmos mixture of the first step; A third step of preparing a culture of SL9-9 strain of Bacillus (Accession No .: KACC 91232P); A fourth step of adding the Bacillus genus SL9-9 strain culture to perlite in step 3; A fifth step of adding and mixing four perlites of 8 to 12% by volume to the mixture of the two steps; And a sixth step of adding and mixing the liquid ratio to the mixture of the five step. The method according to claim 8, wherein the Bacillus genus SL9-9 strain culture of step 4 is a Bacillus genus microorganism containing clay, characterized in that added in a volume ratio of 0.1 to 5 with respect to the volume 100 of the cocoite, peat moss, vermiculite and pearlite mixture Manufacturing method. The method according to claim 8, wherein the liquid ratio of step 6 is added in a volume ratio of 0.1 to 3 with respect to 100 volumes of cocoite, pitmos, vermiculite and pearlite mixture.

Images