KR20090066846A - Formula for anti-aging - Google Patents

Abstract

The mixture for antiaging, which contains Polygonum multiflorum and Cudrania tricuspidata leaf is provided to suppress the aging of brain and liver and inhibit the aging by oxidative stress. A mixture for antiaging containing 40-80% of Polygonum multiflorum and 20-60% of Cudrania tricuspidata leaf based on total weight of mixture. The mixture for antiaging is used in a solid form of powder, pill, capsule and pellet lipid form. The extracts Polygonum multiflorum and Cudrania tricuspidata leaf is obtained by adding purified water, heating at 100°C, filtering, and concentrating the extract. The mixture for antiaging further comprises 0.1-10 weight part of additive based on the 100 weight part. The additive is red ginseng, ginseng, raw ginseng, squalene, asparaginic acid, asparaginic acid, green tea, grape skin, grape seed, resveratrol, vitamin C, vitamin B, tocopherol, mushroom, cordyceps, hoelen, corni Fructus, garlic, black soybean, broccoli, acanthopanacis Cortex, antocyanine, kinds, beta-carotene, sesaminol, sesamol, sesamolin, and mulberry.

Description

Anti-aging Formulations {Formula for anti-aging}

The present invention relates to an anti-ageing formulation, and more particularly, to an anti-aging formulation including sewage and jacuzzi leaves.

As a person ages, aging occurs with degenerative diseases. This aging is an internal change in addition to external phenomena such as decreased skin elasticity, bend of back, dark mushrooms on the face, and loss of physical strength. In the cells, the deposition of pigments and accumulation of small fat globules in cells. , Cell volume decreases, nucleus atrophy, hormone secretion decreases.

There are many causes of aging in humans, and the increase of DNA molecule breakdown in cells over time, the ability to repair DNA molecule disorders, the error of transcription and translation of DNA genetic information, the collagen's legs in vivo Increased binding and decreased immune function have been found to cause aging.

In addition to the above causes of aging, the increase of free radicals is also an important cause of aging.

Cells have several functions to remove free radicals, including enzymes that remove free radicals (superoxide dismutase, SOD). However, some free radicals cannot be removed and destroy DNA (deoxyribonucleic acid), proteins and lipids in cells. Free phases are therefore an important cause of aging-related diseases.

Since the end of the aging phenomenon is the end of life phenomena, a lot of research has been done to suppress aging. Since aging is one of natural phenomena, it cannot be completely overcome, but the progress of aging can be suppressed by ingesting various functional ingredients.

Therefore, efforts have been made to suppress the progress of aging using various functional ingredients.

The present invention relates to an anti-aging compound for slowing the aging process, and more particularly, to an anti-aging compound including sewage and coriander leaves.

Sewage ( Polygonum multiflorum ), a component of the anti-aging formulation of the present invention, is a vine perennial herb with the grasshopper, and in particular, the reddish-brown vine root is called sewage in Chinese medicine and is used as a tonic, tonic, laxative, etc. .

Cuji mulberry leaves of the anti-aging formulations of the present invention is a leaf of Cuji mulberry which is a deciduous tree belonging to the mulberry family. Cudrania is one of the important traditional herbs used in anti-cancer, anti-inflammatory and liver damage in Korea, China and Japan.

The present invention is to provide an anti-ageing formulation, in particular an anti-aging formulation capable of suppressing aging due to oxidative stress.

The present invention, in the anti-aging formulations, to provide an anti-aging formulation comprising sewage, Cudrania leaves.

Anti-aging formulations of the present invention is an anti-aging formulation further comprising an additive in addition to the sewage, kkujippong leaf components.

Anti-aging formulations of the present invention can prevent anti-aging.

Anti-aging formulations of the present invention can inhibit the aging of the brain and liver, according to mouse experiments.

The present invention provides an anti-aging formulation.

The present invention represents an anti-ageing formulation capable of inhibiting aging due to oxidative stress.

Anti-aging formulations of the present invention include sewage, Cudrania leaves.

Sewage in the above may be included 40 to 80% relative to the total weight of the anti-aging formulations, kkujippong leaves may be included 20 to 60% relative to the total weight of the anti-aging formulations.

In the above, the anti-aging formulations, including Shou, Cudrania leaf, can be formulated in a solid phase.

In the above, the anti-aging formulations including sesuo, kkujippong leaves can be formulated in any one selected from powder (powder), pill (capsule), capsule (capsule), pellet (pellet).

In the above, the anti-aging formulation comprising sewage and cuddle leaves, first powdered sewage and cuddle leaves, respectively, with a grinder, and then any one selected from powders, pills, capsules, and pellets from such sewage powder and cuddle leaf powder. It can be formulated in a solid phase. At this time, an excipient may be added to the sewage powder and the zigzag leaf powder to obtain an anti-aging formulation including sewage and zigzag leaf and formulated in any one solid phase selected from powders, pills, capsules, and pellets.

Excipients for obtaining anti-aging formulations formulated in any one solid phase selected from the above powders, pills, capsules, and pellets can be carried out by those skilled in the art, so a detailed description thereof will be omitted.

In the above, the anti-aging formulations including Shou, Cudrania leaf may be formulated in a liquid phase.

In the above, the anti-aging formulations including sewage and cooji mulberry leaves may be formulated in any one liquid selected from hot water extract, organic solvent extract.

The anti-aging formulations comprising sewage and Cudrania leaf may be formulated in a liquid form by mixing sewage hydrothermal extract and Cudrania leaf hydrothermal extract after obtaining sewage hydrothermal extract and Cudrania leaf hydrothermal extract using hot water. .

The sewage hot water extract is added to 3 to 10 times the amount of purified water to sewage, and extracted at 80 to 100 ° C to 10 to 30% of the volume of the first purified water, and then the extract is filtered and the concentration of the filtered extract is It is concentrated and used to 10 to 70 brix.

The Cudrania leaf hot water extract is 3 to 10 times the amount of purified water is added to the Cudrania leaf weight, the extract is extracted to 10 to 30% of the volume of the first purified water at 80 to 100 ℃ and then the extract is filtered, the filtered extract The concentration of is used to be concentrated to 10 to 70 brix.

In the above, the anti-aging formulations containing sewage and Cudrania leaf are obtained in a liquid form by mixing sewage organic solvent extract and Cudrania leaf organic solvent extract after obtaining a sewage organic solvent extract and a Cudrania leaf organic solvent extract using an organic solvent. Can be formulated.

As the organic solvent used when the sewage organic solvent extract, Cudrania leaf organic solvent extract can be used an alcohol solvent having 2 to 7 carbon atoms.

Ethanol may be used as the organic solvent used when the sewage organic solvent extract, Cudrania leaf organic solvent extract.

The sewage organic solvent extract is 3 to 5 times the amount of ethanol added to the sewage, and extracted for 6 to 48 hours at 60 to 70 ℃, then the extract is filtered, the concentration of the filtered extract is 10 to 80 Brix It can be concentrated and used to make brix. At this time, ethanol of 70 to 95% concentration can be used.

The Cudrania leaf organic solvent extract is 3 to 5 times the ethanol is added to the Cudrania leaf weight to the Cudrania leaves, extracted for 6 hours to 48 hours at 60-70 ℃ and then the extract is filtered, the concentration of the filtered extract 10 It can be used concentrated to be -80 brix. At this time, ethanol of 70 to 95% concentration can be used.

In the present invention, the anti-aging compound may further include an additive to the anti-aging compound including the sewage and mulberry leaves in order to improve the effect of anti-aging.

Anti-aging formulations of the present invention, red ginseng, ginseng, fresh ginseng, camphor ginseng, squalene, aspartic acid, chlorella, roobosti, Oolong tea, green tea, grape skin, grape seed, resveratrol, vitamin C, vitamin B, tocopherol, mushrooms, cordyceps, baekbokyeong, cornus, garlic, black beans, broccoli, organo, anthocyanin, eggplant, β-carotene, sesaminol, Sesamol, sesamoline, Audi, creation, may be further included 0.1 to 10 parts by weight of any one or more additives selected from the two worms.

In the anti-ageing formulation of the present invention, the anti-ageing formulations according to the conditions of various components, contents, etc. have been investigated. In order to achieve the object of the present invention, it is preferable to provide the anti-aging formulations under the above-mentioned conditions.

Hereinafter, the contents of the present invention will be described in detail through production examples, examples and test examples. However, these are intended to explain the present invention in more detail, and the scope of the present invention is not limited thereto.

Preparation Example 1 Sewage Extract

Five times the amount of purified water was added to the sewage, and extracted to be 10% by weight of the first purified water at 100 ° C., to obtain a sewage extract, which was filtered.

The filtered sewage extract was concentrated to 60Brix to produce sewage hot water extract.

Preparation Example 2 Preparation of Cudrania Leaf Extract

Five times the amount of purified water was added to the Cudrania leaf weight, and extracted to 100% of the weight of the initial purified water at 100 ℃, to obtain the Cudrania leaf extract and filtered it.

The filtered Cudrania leaf extract was concentrated to 60Brix to prepare Cudrania leaf hot water extract.

<Example 1>

50 wt% of 60Brix sewage extract obtained in Preparation Example 1 and 50 wt% of 60Brix Cudrania leaf extract obtained in Preparation Example 2 were mixed to prepare an anti-aging formulation.

<Example 2>

40 wt% of 60Brix sewage extract obtained in Preparation Example 1 and 60 wt% of 60Brix Cudrania leaf extract obtained in Preparation Example 2 were mixed to prepare an anti-aging formulation.

<Example 3>

60 wt% of 60Brix sewage extract obtained in Preparation Example 1 and 40% by weight of 60Brix Cudrania leaf extract obtained in Preparation Example 2 were mixed to prepare an anti-aging formulation.

<Example 4>

80 wt% of 60Brix sewage extract obtained in Preparation Example 1 was mixed with 20 wt% of 60Brix Cudrania leaf extract obtained in Preparation Example 2 to prepare an anti-aging formulation.

<Example 5>

50 wt% of 60Brix sewage extract obtained in Preparation Example 1 and 50 wt% of 60Brix Cudrania leaf extract obtained in Preparation Example 2 were mixed to prepare an anti-aging formulation.

By adding 5 parts by weight of cornus extract as an additive with respect to 100 parts by weight of the anti-aging formulations containing sewage, jacuzzi leaves, an anti-aging formulation containing sewage, custard leaves and cornus milk was prepared.

The cornus extract was prepared by the same method as Preparation Example 1, except that cornus oil was used instead of sewage.

<Test Example> Test of anti-aging activity of a sample in mice induced by aging with D-galactose (see Biogerontol. 4, 15 (2003))

(1) Experimental method

Superoxide dismutase (SOD) and total antioxidant status (TAS) test kits were purchased from RANDOX (UK).

Acetylthiocholine iodide, DTNB, trichloroacetic acid (TCA), thiobarbituric acid (TBA) and D-galactose were purchased from Sigma.

The March C57 BL / 6J male mice were purchased from Daehan Biolink in Chungbuk. Mice were daily for 45 days: ① 0.3 ml PBS as vehicle control; ② D-galactose at 0.5g / kg (5% D-galactose, aging control) was injected subcutaneously. Mice were slaughtered on the last day of the test and blood, serum and organs were stored at ?? 70 ° C.

Sewage extract of Preparation Example 1, Kujippong leaf extract of Preparation Example 2 and the sample mixed with Sewage extract and Kujippong leaf extract of Example 1 was orally administered twice a day at 10 and 17 o'clock, the daily dose of the sample is weekly The weight of the rats was measured and converted from the daily intake of humans and the average metabolic weight ( ^{3/4 of} body weight) between humans and rats (Nutr. Res. 11, 1465 (1991)). 1.8g of extract, 1.5g of Cudrania leaf extract, 3.3g of sewage and Cudrania leaf extract combination (Kim Changmin et al., Chinese Traditional Chinese Dictionary, Book Publishing Jungdam (1998)), and the average body weight of human were calculated as 65kg. Rat body weights were measured weekly.

SOD activity of erythrocytes in mice was measured by RANSOD (RANDOX) to measure the dismutation of toxic superoxide radicals. A standard curve of free radical suppression was drawn in the range of 30 to 60%. Liver and brain were used to make 10% homogenate with PBS.

Total antioxidant status of the serum was measured by RANDOX kit.

To measure MDA (malondialdehyde), TCA (15ml, 100%), 0.375g TBA, 25ml HCl (1N) and 40mg BHT (butylated hydroxytoluene) / ethanol were mixed and redistilled water to 100ml. Was added to make a TBA solution. 10% PBS homogenate of liver and brain was mixed with 1 ml TBA solution, 0.5 ml sample and 15 µl of 50 mM BHT were reacted at 100 ° C. for 15 minutes and cooled with ice water. Malondialdehyde was quantified by centrifugation at 3000 rpm for 30 minutes and absorbance at 535 nm. Blood was used as it is.

Protein concentration was determined by the Bradford method (Bio-Rad) using bovine serum albumin. Hemoglobin concentration was measured with the SIGMA 525-A kit.

The measurements were analyzed by Student's t-test or Duncan's multiple range test.

(2) Experimental results

No visual abnormality was found in all groups and normal weight gain was shown. Table 1 shows the results of malondialdehyde (MDA), superoxide dismutase (SOD) and total antioxidant status (TAS) as blood biomarkers.

MDA is an indicator of lipid peroxidation, SOD is an enzyme that reduces free radicals, and TAS increases early in oxidative stress.

Aging controls treated with 5% D-galactose increased serum MDA and TAS and decreased SOD compared to normal controls ( P < 0.05). Oxidative stress caused by increased MDA and decreased SOD is known to cause D-galactose aging (Cell Mol Neurobiol. Aug 21 (2007) Temporal Gene Expression Profile in Hippocampus of Mice Treated with D -galactose). Ingestion of the mixture of sewage extract and Cudrania leaf extract improved the blood biochemical index induced by 5% D-galactose. In the sewage extract group, SOD was improved to the same level as the normal control group, and in the Cudrania leaf extract group, the TAS decreased to the same level as the normal control group. Particularly, in the example group in which 50% of sewage extract and 50% of kkujippong leaf extract were combined on the basis of weight, all the measurements of MDA, SOD and TAS were improved to the same level as the normal control, so that the combination of the two extracts was caused by oxidative stress. It is shown that it can effectively suppress the aging of the.

Table 1 . MDA, SOD and TAS measurements of mouse blood ¹

Item MDA ( μ mol / g protein) SOD (U / mg Hb) TAS (mM) Normal control 1.02 ± 0.16 ^a 136.81 ± 31.83 ^b 0.17 ± 0.09 ^a Aging Control 1.53 ± 0.22 ^b 71.94 ± 18.35 ^a 0.43 ± 0.15 ^b Sewao Extract 1.26 ± 0.21 ^ab 114.74 ± 16.24 ^b 0.24 ± 0.10 ^ab Cudrania Leaf Extract 1.14 ± 0.13 ^a 97.34 ± 15.44 ^ab 0.27 ± 0.15 ^ab Example 1 1.09 ± 0.17 ^a 123.74 ± 16.01 ^b 0.20 ± 0.07 ^a

¹ mean ± SEM, 10 per group. Values with different superscripts in the same column are significantly different ( P <0.05).

Table 2 investigates the effect of the mixing ratio of sewage extract and Cudrania leaf extract on MDA and SOD of mouse brain tissues for the anti-aging formulations of Examples 2, 3 and 4. In the aging control group, MDA increased and SOD decreased compared to the normal control group ( P < 0.05). When the anti-aging compound of Example 3 (mixture ratio of sewage extract and kkujippong leaf extract is 60:40) was the best anti-aging activity, both MDA and SOD improved to the same level as the normal control. The anti-ageing formulation of Example 2 and the anti-aging formulation of Example 4 also improved MDA and SOD levels, but there was no statistically significant difference.

Table 2 . Effect of Sewage and Cudrania Leaf Extract Ratio on MDA and SOD of Brain Tissue ¹

Item MDA ( μ mol / g protein) SOD (U / mg protein) Normal control 10.71 ± 1.14 ^a 14.87 ± 1.25 ^b Aging Control 13.85 ± 2.49 ^b 12.76 ± 1.89 ^a Example 2 11.27 ± 1.38 ^ab 13.05 ± 1.94 ^a Example 3 10.89 ± 1.26 ^a 14.41 ± 1.91 ^b Example 4 11.61 ± 1.34 ^b 13.67 ± 1.70 ^ab

¹ mean ± SEM, 10 per group. Values with different superscripts in the same column are significantly different ( P <0.05).

Table 3 examines the effects of Shou extract and Cudrania leaf extract on the MDA and SOD of mouse liver tissues for different anti-aging formulations.

The anti-aging compound of Example 2 (mixture ratio of sewage extract and kkujippong leaf extract was 60:40) had the best anti-aging activity, and the MDA decreased to the same level as the normal control, and the SOD was slightly increased than the normal control. The anti-ageing formulation of Example 3 and the anti-aging formulation of Example 4 also improved MDA and SOD levels, but there was no statistically significant difference.

Table 3 . Effect of Sewage and Cudrania Leaf Extract on the MDA and SOD of Liver Tissue ¹

Item MDA ( μ mol / g protein) SOD (U / mg protein) Normal control 0.51 ± 0.09 ^a 17.84 ± 3.25 ^a Aging Control 0.68 ± 0.13 ^b 17.76 ± 4.89 ^a 40: 60 0.59 ± 0.12 ^ab 17.83 ± 3.94 ^a 60: 40 0.53 ± 0.10 ^a 19.99 ± 2.89 ^b 80: 20 0.61 ± 0.14 ^b 17.87 ± 3.70 ^a

¹ mean ± SEM, 10 per group. Values with different superscripts in the same column are significantly different ( P <0.05).

As described above, although described with reference to a preferred embodiment of the present invention, those skilled in the art will be variously modified and modified within the scope of the present invention without departing from the spirit and scope of the invention described in the claims below. It will be appreciated that it can be changed.

The anti-aging compound of the present invention can be used as it is to prevent anti-aging, as well as to provide a product having an anti-aging effect by using the anti-aging compound for food, cosmetics, and the like.

Claims (5)

In anti-aging formulations, Sewage, anti-aging formulations, characterized in that it comprises cooji leaves. The anti-aging compound according to claim 1, wherein the sewage is contained 40 to 80% of the total weight of the anti-aging compound.  The anti-aging formulation according to claim 1, wherein the zigzag leaf is comprised in 20 to 60% of the total weight of the anti-aging formulation. The anti-aging formulation of claim 1, wherein the blend comprising sewage and cudica leaves is in the form or extract of any one solid form selected from powders, pills, capsules, and pellets. Anti-aging formulations, characterized in that the liquid formulation. According to claim 1, red ginseng, ginseng, ginseng, camphor ginseng, squalene, aspartic acid, chlorella, rooibos tea, oolong tea, green tea, grape skins, grape seeds, resveratra Troll, Vitamin C, Vitamin B, Tocopherol, Mushroom, Cordyceps Sinensis, Baibok-ryeong, Cornus, Garlic, Black Bean, Broccoli, Ogapi, Anthocyanin, Eggplant, β-carotene, Sesaminol, Sesamol, Sesamoline, Ody, Produce, Anti-aging formulations further comprises 0.1 to 10 parts by weight of any one or more additives selected from the two worms.