KR20090029143A - Cosmetic composition with the effect of calming skins - Google Patents

Abstract

A cosmetic composition is provided to ensure scalp protection and skin sedation effect by comprising fermentation extract of black beans, black sesamum indicum and black rice. A cosmetic composition having scalp protection and skin sedation effect comprises 0.1-15 weight%, preferably, 2 weight% of the fermentation extract of black beans, black sesamum indicum and black rice. A method for preparing the fermentation extract of black beans, black sesamum indicum and black rice comprises the steps of: mixing black beans, black sesamum indicum and black rice in a weigh ratio of 60:20:20; adding a mixture of 0.1-20.0 weight% in the culture fluid; producing a polymer by fermenting Bacillus species microorganism in the fermentation condition of 30-60°C, 5.5-8.5 pH and facultative anaerobe fermentation in the culture fluid; mixing the cultivating supernatant removed with fungi and ethanol in a volume ratio of 1:2; controlling the pH of the mixed solution by using 1N hydrochloric acid solution; collecting and re-dissolving the precipitate by mixing the mixed solution gradually to obtain the first refined material; and removing the low molecular weight substances and pulverizing, by dialyzing the refined material through a film filter of the molecular weight 10kD at 4°C.

Description

Cosmetic composition with the scalp protection and skin calming effect {Cosmetic composition with the effect of calming skins}

The present invention relates to a cosmetic composition having a scalp protection and skin soothing effect, and more particularly, a cosmetic that can provide scalp protection and skin soothing effect by containing fermented extracts of black beans, black sesame seeds and black rice as main natural ingredients. It relates to a composition.

In general, the skin may be sensitive to external stimuli such as a dry environment. In some cases, this may cause a temporary erythema, but it may also cause more complex problems such as severe itching and stinging, which may lead to an inflammatory response in appearance.

The mechanism of skin irritation is not known, but it is known that the most important defense function of the skin is abnormal and irritants penetrate into the skin and cause inflammation. Skin irritants also abnormally induce the function of intracellular metabolism and the pain associated with stimulation further increases the inflammatory response.

In an effort to prevent such skin irritation, research has been conducted on anti-irritant cosmetics, and in recent years, research and development on cosmetics using natural vegetable raw materials have been actively conducted in response to consumer demand.

The present invention is to solve the above problems, it is to provide a cosmetic with a soothing skin protection and skin soothing effect using a fermented extract of black beans, black sesame seeds, black rice as a natural ingredient for skin protection.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a cosmetic composition having scalp protection and scalp protection and skin soothing effect, and more particularly, contains black soybean, black sesame and black rice fermented extract as main natural ingredients to have skin protection and soothing effect. It has a high absorbance, good solubility, affinity, and stability in various bases and additives for cosmetics, organic solvents, and the like, and particularly, when used in combination with existing sunscreens, it provides a synergistic effect of significantly increasing the sunscreen index.

As a main natural component in the composition according to the present invention is used in combination in an amount of 0.1 to 15% by weight based on the dry weight of black bean, black sesame, black rice fermented extract.

The present invention is a black bean, black using a Bacillus microorganism under the culture conditions containing 0.1 to 20.0% by weight of black beans, black sesame, black rice at 30 to 60 degrees C, pH 5.5 to 8.5, and anaerobic fermentation conditions Fermented mixture of sesame seeds and black rice to produce a polymer material, and then the sterilized culture supernatant and ethanol are added in a volume ratio of 1: 2, calibrated to about pH 3 with 1N hydrochloric acid solution, and slowly stirred to recover the precipitate and reused. The residue was purified first. For further purification, low-molecular-weight substances can be removed by dialysis using a membrane filter with a molecular weight of 10 kD at 4 ° C. Distilled water in the same volume as the separated solution is added to lower the viscosity of the polymer material and then lyophilized to determine dry weight to determine productivity.

The extract thus prepared is added during cosmetic preparation.

The characteristics of the genus Bacillus strains are gram-positive bacteria, endogenous spore-forming ability, and has the form of enterococci. In addition, it is possible to grow under aerobic or anaerobic conditions, but not under anaerobic conditions, and has the characteristics of catalase production, casein, gelatin, and starch resolution. As the growth conditions, it is possible to grow at 7% saline, and it is possible to grow at about 45 ° C, which is a fermentation condition, and to grow at about pH 5.0 to 7.5. In addition, glucose, arabinose, xylose, and mannitol has the characteristic of generating an acid.

The cosmetic composition according to the present invention is characterized in that the prepared soybean, black sesame, black rice fermented extract to 0.1 to 15% by weight, preferably 2% by weight.

Cosmetic composition having scalp protection and scalp protection and skin soothing effect according to the present invention may have a conventional formulation, for example, supple cosmetics, astringent cosmetics, nourishing cosmetics, nutrition cream, body lotion, body oil, body essence, etc. It can be formulated as. The compositions of each of these formulations may contain various bases and additives necessary for the formulation of the formulations and are suitable, and the types and amounts of these components can be readily selected by those skilled in the art. Representative skin and lotion compositions of cosmetic compositions having scalp protection and scalp protection and skin soothing effects are described below by way of example.

In addition, the cosmetic composition having a scalp protection and scalp protection and skin soothing effect according to the present invention may be used according to a conventional method of use, and the number of times of use may vary depending on the user's skin condition or taste.

Hereinafter, the cosmetic composition having the scalp protection and scalp protection and skin calming effect of the present invention will be described in more detail with reference to the present invention, but the present invention is not limited to these examples.

Example 1 Preparation of Black Bean, Black Sesame, Black Rice Fermented Extract

Black beans, black sesame seeds, black rice extract was used to dry fine powder.

In the above embodiment, the black beans, black sesame seeds, and black rice are mixed at 60:20:20 at a temperature of 30 to 60 ° C., at a pH of 5.5 to 8.5, and in anaerobic fermentation conditions, respectively, at 0.1 to 20.0% by weight. Under the conditions of the culture medium to ferment the mixture of black beans, black sesame seeds, black rice using a Bacillus microorganism to produce a polymer material, and then the sterilized culture supernatant and ethanol are added in a volume ratio of 1: 2 and 1N hydrochloric acid solution to about pH 3 After correcting, the mixture was slowly stirred to recover the precipitate, which was re-dissolved and purified first. For further purification, the low molecular weight material was removed by dialysis using a membrane filter having a molecular weight of 10 kD at 4 ° C. Distilled water in the same volume as the separated solution was added to lower the viscosity of the polymer material and then lyophilized to produce black soybeans, black sesame seeds and black rice at 30 to 60 ° C, pH 5.5 to 8.5, and anaerobic fermentation conditions. : 20: 20 and fermented a mixture of black beans, black sesame seeds, black rice using a Bacillus microorganism under a culture solution containing 0.1 to 20.0% by weight to produce a polymer material and then the culture supernatant and ethanol 1 The mixture was added at a volume ratio of 2: 2, calibrated to about pH 3 with 1N hydrochloric acid solution, and stirred slowly to recover the precipitate, which was then redissolved and purified first. For further purification, low-molecular-weight substances can be removed by dialysis using a membrane filter with a molecular weight of 10 kD at 4 ° C. Distilled water in the same volume as the separated solution was added to lower the viscosity of the polymer material and then lyophilized to prepare fermented extracts of black beans, black sesame seeds and black rice.

Example 2 preparation of skin lotion having scalp protection and scalp protection and skin soothing effect according to the present invention

A skin lotion was prepared at the composition ratio as shown in Table 1 below. The alcohol part was produced by dissolving in the order of MP, tween 20, NP-12 in alcohol distilled at 78 ° C in the order of grain, and the purified water part was glycerin, 1,3-BG, DC-193, hyaluronic acid in sterilized purified water. Prepared by dissolving in the order of, allantoin, citric acid, sodium citric acid, and then mixed with black bean, black sesame, black rice fermented extract prepared in Example 1 to prepare a skin lotion.

Classification Raw material name Weight (% by weight) Alcohol part ethanol 7 Methylparahydroxybenzoic acid 0.1 tween-20 0.2 Nonylphenylether-12 0.2 Purified water Purified water 89.51 glycerin 0.1 1,3-butylene glycol 0.2 Alanine 193 0.1 Hyaluronic acid 0.5 Allantoin 0.05 Citric acid 0.01 Citric acid 0.03 Natural ingredients Fermented extract 2 Sum 100

Example 3: Preparation of milk lotion having scalp protection and scalp protection and skin soothing effect according to the present invention

Milk lotion was prepared in the same manner as in Example 2.

Classification Raw material name Weight (% by weight) Alcohol part Stearic acid 2.1 Cetanol 1.3 Liquid paraffin 7.0 Squalene 0.5 Isopropyl myristate 1.0 Glyceryl Monostearate 0.5 Arasel-60 0.85 tween-20 0.1 Isostearyl-isostearate 0.5 Propylparaben 0.1 Alanine 200F / 100CS 0.1 Purified water Purified water 73.65 Triethanolamine 0.7 Propylene glycol 7.0 glycerin 3.0 Methylparahydroxybenzoic acid 0.1 Natural ingredients Fermented extract 1.5 Sum 100

Experimental Example 1: Check the skin irritation and calming effect of the skin lotion according to the present invention

Apply the skin lotion prepared in Example 2 (10 m / 5 cm 2) to the forearm of 20 healthy men and women (10 males and 10 females) and shield the rest with aluminum foil for 30 minutes. Subsequently, long wavelength ultraviolet rays were irradiated at a dose of 10.2 J / cm 2 using an ultraviolet irradiator, and skin conditions (erythema, edema, papules, etc.) were observed after 24 hours. As a result, skin side effects such as erythema do not occur on the skin, and thus the skin lotion has no phototoxicity, and it has been confirmed that the scalp protection, scalp protection, and skin soothing effect.

Experimental Example 2: Confirmation of skin irritation and calming effect of milk lotion according to the present invention

Apply the milk lotion prepared in Example 3 (10 m / 5 cm 2) to the forearm of 20 healthy males and females (10 males, 10 females) and cover the rest with aluminum foil for 30 minutes. Subsequently, long wavelength ultraviolet rays were irradiated at a dose of 10.2 J / cm 2 using an ultraviolet irradiator, and skin conditions (erythema, edema, papules, etc.) were observed after 24 hours. As a result, the side effects such as erythema do not occur on the skin, and thus the milk lotion has no phototoxicity and has a scalp protection and skin calming effect.

Experimental Example 3: Skin stability confirmation test of skin lotion and milk lotion according to the present invention

The skin patch and milk lotion prepared in Examples 2 and 3 were subjected to a human patch test on the forearm of 20 healthy adults (10 males and 10 females). Wipe the forearm of the subject well with 70% ethanol, and then place a pin chamber containing 20 μl of the skin lotion and milk lotion prepared in Examples 2 and 3 on a conventional cosmetic base using adhesive tape for 24 hours. Closed attachment. After 24 hours, the adhesive tape and the pin chamber were removed, and the sample remaining on the skin with a gauze was lightly removed, and then the skin condition was observed, and again after 24 hours. Experimental results As shown in Table 3, the human patch test to confirm the skin irritation of the skin lotion and milk lotion according to the present invention did not show a positive reaction was confirmed that there is no irritation to the human skin.

sample After 24 hours (n = 20) After 48 hours (n = 20) Remarks Skin lotion 0/20 0/20 Number of reaction quantums / total number of targets Milk lotion 0/20 0/20

To prepare a shampoo composition with reference to the components and contents shown in Table 4. In the case of the embodiment by mixing a certain amount of fermented extracts of black beans, black sesame seeds, black rice had an excellent scalp protection effect in addition to the cleaning effect, thereby having the effect of delaying hair loss caused by acne.

Shampoo Cosmetic Composition (Unit: wt%) Raw material name Comparative Example 1 Example 4 Lauryl diethanolamide 6.0 6.0 Sodium polyoxyethylene lauryl ether sulfate 30.0 30.0 Sodium Lauryl Sulfate Solution 15.0 15.0 Propylene glycol 1.0 1.0 Methyl Parahydroxybenzoate 0.2 0.2 Fermented extract - 4.0 Pigment 0.5 0.5 Spices 0.5 0.5 Citric acid 0.1 0.1 Purified water Remaining amount Remaining amount Sum 100 100

Experimental Example 4: Determination of cleaning power

Thirty male and female testers with dandruff and itching were observed after 1 day, 3 days, and 7 days, according to the following determination method.

Satisfaction judgment standard Judgment Skin irritation degree -2 Comparative example is very good -One Comparative example is slightly good 0 No difference One Example is slightly good 2 Example is very good

The satisfaction of each subject was measured, and the average satisfaction was calculated by dividing by the number of subjects and is shown in Table 6 below.

Cleaning force measurement result sample Cleaning power 1 day later 3 days later 7 days later satisfaction 1.1 1.3 1.6

In the comparison between Example 4 and Comparative Example 1, the product according to Example 4 of the present invention was found to have a significant difference in satisfaction.

Experimental Example 5 Measurement of the Itching Effect of the Scalp

Twenty men and women with itching due to the occurrence of acne were observed for seven days after the seven days after the itching effect of the scalp.

Criteria for eliminating itching of the scalp (10 points maximum, 1 point minimum) score evaluation 10-8 points Very good (****) 7.9-6 points good(***) 5.9-4 points There is a little (**) 3.9-2 points is average(*) 1.9-1 points none

The satisfaction of each subject was measured, and the results are shown in Table 8.

Satisfaction of itching effect of scalp division Comparative Example 1 Example 4 Itching effect of scalp * ***

As shown in Table 5, it was confirmed that the cleaning composition prepared by the fermentation extract of black beans, black sesame seeds, and black rice with respect to the itch removal effect of the scalp was superior to the above items in comparison with the conventional products. In other words, the above-mentioned effect is a natural bioactive substance obtained from a natural fermentation extract consisting of black soybean, black sesame, and black rice, which is obtained by supplying an appropriate amount of the maximum functional active ingredient, and has an excellent scalp protection effect in addition to the cleaning effect. This can delay the hair loss caused by acne, which can be expected to ultimately prevent hair loss by improving the itching of the scalp.

The cosmetics of the present invention through the above Examples and Experimental Examples to provide a cosmetic composition excellent in scalp protection and skin soothing effect by containing black beans, black sesame, black rice fermentation extract of natural ingredients.

Claims (2)

As a natural ingredient having scalp protection and skin soothing effect, fermented extract consisting of black beans, black sesame seeds, and black rice is contained in an amount of 0.1 to 15% by weight, preferably 2% by weight. Eggplant cosmetic composition The method of claim 1, Fermented extract consisting of black soybeans, black sesame seeds, and black rice is mixed 60:20:20 black beans, black sesame seeds, and black rice at 30 to 60 degrees Celsius at temperature, 5.5 to 8.5 at pH and anaerobic fermentation conditions. Fermentation of black soybeans, black sesame seeds, and black rice mixtures using Bacillus microorganisms under culture conditions containing 0.1 to 20.0% by weight to produce a polymeric material, followed by adding the sterilized culture supernatant and ethanol in a volume ratio of 1: 2. After calibrating to pH 3 with 1N hydrochloric acid solution, slowly stirring to recover the precipitate, re-dissolving it, and purifying it firstly, and removing the low molecular weight material through dialysis using a membrane filter having a molecular weight of 10 kD at 4 ° C. Scalp protection and skin soothing effect, characterized in that it comprises a fermented extract consisting of black beans, black sesame, black rice, which is composed of a powdering step Eggplant cosmetic composition