KR20090014528A - Composition including natural organic minerals of deep sea water and green tea functional components - Google Patents

Abstract

A green tea extract composition is provided to increase the content of the effective ingredient of green tea and the content of mineral components. A green tea extract composition comprises 5-30 wt% of a green tea extract; and 70-95 wt% of an extraction solvent comprising deep sea water. Preferably the extraction solvent has a hardness of 100-13,000. Preferably the extraction solvent comprises 5-70 wt% of a solvent selected from the group consisting of purified water, an organic solvent, and their mixture; and the balance of deep sea water.

Description

Composition including natural organic minerals of deep sea water and green tea functional components

The present invention relates to a green tea extract composition, and more particularly, to a green tea extract composition having a high content of green tea extract and minerals such as polyphenol-like functional substances.

Green tea ( Camellia Sinensis L.) is a perennial seed plant belonging to the tea tree family. It is known to have many physiological effects. Green tea contains various useful ingredients that exhibit the above-mentioned effects. Typically, green tea contains a large amount of physiologically active polyphenols such as catechin, tannin, caffeine, chlorophyll, Vitamins A, B1, B2, C, E and amino acids, proteins, fats, and fiber are included. Green tea is popularly used for beverages and skin beauty.The green tea leaves are suitable for drinking as raw materials, and are processed by heating, grinding, and drying. It is also used as a raw material for food and cosmetics after drying.

However, when the green tea is simply extracted with a common extraction solvent such as water, methanol, ethanol, etc., the extraction efficiency of the green tea active ingredient is low, and the extraction process must be repeatedly performed, or only a part of the active ingredient is extracted and a large amount of the active ingredient is extracted. There is a problem of discarding the remaining green tea. In addition, when the green tea is extracted using a large amount of solvent, or the extraction process is repeatedly performed, a considerable cost and effort is required to concentrate the extract, which is not suitable for the industrial mass production of green tea extract. In addition, in the case of conventional plant extracts such as green tea, there is a disadvantage that the content of minerals is small.

Accordingly, an object of the present invention is to provide a green tea extract composition containing a large amount of minerals, as well as green tea extracts such as polyphenol functional substances.

Another object of the present invention is to provide a green tea extract composition from which the green tea active ingredient is efficiently extracted.

Still another object of the present invention is to provide a green tea extract composition which can be used for various purposes.

In order to achieve the above object, the present invention, green tea extract 5 to 30% by weight; And it provides a green tea extract composition comprising 70 to 95% by weight of the extraction solvent including deep sea water. Here, the hardness of the extraction solvent is 100 to 13,000, the extraction solvent is preferably composed of 5 to 70% by weight of the solvent selected from the group consisting of purified water, organic solvents and mixtures thereof and the remaining deep sea water.

Hereinafter, the present invention will be described in more detail.

The green tea extract composition according to the present invention includes a green tea extract and an extracting solvent, and the extracting solvent not only extracts the green tea active ingredient, but also includes deep sea water for supplying minerals. Generally, deep sea water means seawater located in the deep sea of more than 200m deep where sunlight does not reach, and the deep-water council of the Japan Fisheries Agency does not produce organic matter by photosynthesis, and does not produce vertical mixing in winter. Deep sea water is defined as deep water. These deep sea waters are rich in inorganic nutrients necessary for life activities, rich in nutrients, cleanliness free from chemical contamination, low temperature stability with little temperature change, It has characteristics such as maturation matured over a long period of time at a pressure of 20 atm and higher, including fisheries (aquaculture), energy (cooling), product fields (food, salt, liquor, bottled water, cosmetics), and medical care. It is widely used in the field (atopic skin treatment) and the like. Aging of deep sea water means that deep sea water is “very fine” and “soft” compared to surface water, which means that deep water is denser due to low temperatures and high pressures, and fine granular minerals can It is dissolved in. In addition, since deep ocean water is not contaminated with artificial materials and photosynthetic action does not occur, there is a characteristic that there is little organic material, pathogens and bacteria to be decomposed and is very clean. In particular, since the deep sea water contains four major minerals of magnesium, calcium, potassium, and sodium, various minerals such as zinc, selenium, and manganese, and essential trace elements, they are useful for the production of mineral water through desalination. have.

Extraction solvents that can be used in the composition of the present invention include raw water, which is deep sea water taken out, concentrated water in which the ionic components of the raw water are concentrated, permeated water in which the ionic components are reduced from the raw water, and mixtures thereof And the like, and preferably, deep sea water having a hardness of 100 to 13,000, more preferably 1,000 to 12,000, and most preferably 3,000 to 11,000 can be used. In general, the depth of the deep sea water is about 6,300, and the hardness of the concentrated water may vary depending on the depth of the deep sea water, but is about 10,000 to 13,000. The deep sea water not only significantly improves the extraction efficiency of the green tea active ingredient, but also acts as a mineral source, compared to conventional extraction solvents such as purified water, methanol and ethanol. If the hardness of the deep sea water is too low, the green tea extraction efficiency is lowered, there is a fear that the green tea active ingredient and mineral content in the green tea extract composition is not enough, and if the hardness is too high, there is a fear that the economic feasibility to industrialize in reality. As the concentrated water and the permeated water, concentrated water and permeated water generated while removing sodium chloride from raw water may be used by conventional methods such as reverse osmosis and electrodialysis. The depth of the deep sea water may include the raw water, It can be adjusted by appropriately mixing two or more kinds selected from the group consisting of concentrated water, permeated water, purified water, organic solvents and the like. For example, raw water: filtrate: concentrated water may be used as an extraction solvent by mixing in a volume ratio of 1: 0.2 to 2: 0.2 to 3, preferably 1: 0.5 to 2: 0.5 to 3, and to the mixing ratio of the extraction solvent. Accordingly, since the components and contents of the minerals included in the extraction solvent are different, it is possible to adjust the amount of extraction of a specific active ingredient from green tea. In addition, in the extraction solvent of the present invention, the content of sodium ions can be reduced to 50 parts by weight or less, preferably 5 to 30 parts by weight or less with respect to 100 parts by weight of magnesium ions, and thus the content of sodium components It is preferable to reduce the saltiness of the extraction solvent. Extraction solvents having a reduced content of sodium components can be prepared by known methods, for example, as described in the applicant's patent application 10-2006-0054899.

As the purified water used together with the deep sea water, conventional distilled water, tap water, bottled water, etc. may be used without particular limitation, and as the organic solvent, lower (carbon number 1 to 4) alcohols such as methanol and ethanol, and lower Organic acids, lower alcohol esters, lower ketones, halogenated hydrocarbons, mixtures thereof and the like can be used. When the purified water and / or the organic solvent is used, the content can be freely controlled within the limit of maintaining the hardness of the deep sea water at 100 to 13,000, preferably 0 to 80% by weight, preferably based on the total extraction solvent. Preferably from 5 to 70% by weight, more preferably from 10 to 30% by weight. The remaining components of the extraction solvent other than purified water and / or organic solvent are deep sea water such as raw water, concentrated water and permeated water. If the content of the purified water or the organic solvent is too small, the hardness of the extraction solvent may be too high, if too much, there is a fear that the extraction efficiency is lowered. After extraction of the green tea active ingredient, the organic solvent may be removed by heating or the like as necessary.

The green tea extract included in the composition of the present invention contains polyphenol compounds such as catechin and tannin, free amino acids, free sugars, saponins, organic acids and the like as the active ingredients. caffeine), chlorophyll (chlorophyll), vitamins (vitamin) A, B1, B2, C, E and protein, fat, fiber and may further include.

In the green tea extract composition according to the present invention, the green tea portion including the green tea active ingredient, such as green tea leaves, is added to the extraction solvent containing the deep sea water, the green tea active ingredient is extracted, and the green tea from which the active ingredient is removed is filtered. It can be prepared by filtration. The extraction process may be carried out at pH 3 to 10, temperature 1 to 25 ℃, preferably 2 to 10 ℃, 2 hours to 10 days, preferably 1 to 7 days. If the extraction condition is out of the above range, the extraction efficiency of the green tea active ingredient is lowered, or only economically disadvantageous. In the green tea extract composition according to the present invention, the content of the extraction solvent is 70 to 95% by weight, preferably 75 to 85% by weight, and the content of the green tea extract is 5 to 30% by weight, preferably 15 to 25% by weight. to be. Here, the content of the green tea extract is measured by drying the green tea extract composition. In the green tea extract composition of the present invention, when the content of the extraction solvent is too much, the extraction efficiency is increased, but the content of the active ingredient is small, the extraction and production cost of the active ingredient is increased, and when the content of the extraction solvent is too small, extraction There is a disadvantage that the efficiency is reduced.

The green tea extract composition according to the present invention contains not only the active ingredient of green tea but also a large amount of mineral components of deep sea water, and is dried or diluted as it is or as needed, such as beverages, foods, drugs, cosmetics, and the like. An appropriate amount of extracts and minerals can be mixed and used.

The green tea extract composition according to the present invention is a marine natural organic mineral composition in which the green tea active ingredient is efficiently extracted and combines green tea functional ingredients, and contains not only a large amount of green tea extract but also a large amount of minerals, beverages, foods, and the like. It can be used for various purposes, such as medicine, cosmetics, and the like.

Hereinafter, the present invention will be described in more detail with reference to Examples and Comparative Examples. The following examples illustrate the present invention and the present invention is not limited by the following examples.

Production Example 1 Deep Sea Water Collection and Purification

The deep ocean water collected at 500m depth in the East Sea of Korea is finely filtered with a micro filter (material: Polytetrafluoroethylene (PTFE), pore size: about 0.5㎛, Saehan Co., Ltd.) to remove impurities, and then reverse osmosis (Dow Chemical Company). Product, FILMTEC, SW30-4021), and separated into reverse osmosis concentrated water and reverse osmosis filtered water. In addition, the filtered deep sea water (raw water), electrodialysis (electrodialysis, MICRO ACILYZER G4SK, anion membrane: NEOSEPTA AMX SB, cationic membrane: ACIPLEX K-192 / ASAHI KASEI CORPORATION, JAPAN, operating conditions: current density 2 5 A / m ² , STACK voltage 38V constant voltage operation / circulating flow rate 13.1L / min) was separated into electrodialysis concentrated water and electrodialysis filtered water. The content of minerals in the deep sea water (raw water), concentrated water and filtered water is shown in Table 1 (ND: Not detected).

Element (ppm) enemy Reverse Osmosis Filtrate Reverse Osmosis Concentrated Water Electric dialysis Electrodialysis concentrate Al 0.28 0.004 0.56 0.008 0.57 As ND ND ND 0.002 0.002 Ba ND ND ND ND ND Ca 419.0 0.006 687.9 9.7 713.7 CD ND ND ND ND ND Co 0.52 ND 1.6 ND 2.0 Cr 1.9 0.006 0.026 1.9 3.9 Cu 0.053 0.004 2.4 0.007 1.7 Fe 0.005 0.001 1.6 ND 1.5 Mg 1285 0.04 2586 118 2258 Mn 0.043 ND 0.053 ND 0.061 Pb <0.001 <0.001 <0.001 ND ND Sr 6.61 ND ND 0.46 1.65 K 430 5.7 560 1.78 461 Zn 0.01 0.002 0.23 0.002 0.31 Hardness 6316 0.18 12,322 507 11,042

Examples 1 to 3 Preparation of Green Tea Extract Composition

As deep sea water, raw water obtained in Preparation Example 1, concentrated water (a mixture of reverse osmosis concentrate and electrodialysis concentrated water in a 1: 1 volume ratio) and filtrate (reverse osmosis filtered water and electrodialysis water in a 1: 1 mixture) Each), 50 ml of ethanol was mixed with 50 ml of deep sea water, and 10 g of green tea leaves were added to prepare an extraction solution. After leaving the extraction solution at pH 3-10 for 4 days at 4 ° C., the green tea leaf from which the active ingredient was extracted was filtered and removed by heating with ethanol to prepare a green tea extract composition.

Comparative Example 1 Preparation of Green Tea Extract Composition

Green tea extract composition was prepared in the same manner as in Example 1, except that purified water was used instead of deep sea water as the extraction solvent.

Experimental Example 1 Component Analysis of Green Tea Extract Composition

In the green tea extract composition of Examples 1 to 3 and Comparative Example 1, the content of the green tea active ingredient was measured and shown in Table 2 (unit: parts by weight).

Example 1 (raw water) Example 2 (concentrated water) Example 3 (filtration, permeate) Comparative Example 1 (purified water) Free amino acids 6.5 13.4 2.3 0.5 Polyphenols 36.0 44.0 33.0 10.2 Glass sugar 9.0 16.4 10.6 4.2 Saponins 1.1 2.6 0.05 0.03 Organic acid 3.4 7.3 1.7 1.0

As shown in Table 2, in the case of the green tea extract composition including deep sea water, that is, raw water, concentrated water and permeated water, compared to the green tea extract composition containing purified water, the content of the green tea active ingredient is significantly higher. have.

[Comparative Examples 2-3, Examples 4-6] Preparation of Green Tea Extract Composition

The solvent shown in Table 3 below was mixed with green tea in a weight ratio of 10 times the green tea to prepare an extraction solution. The extraction solution was allowed to stand at room temperature for 24 hours to extract the green tea active ingredient, and then the green tea leaf from which the active ingredient was extracted was filtered under reduced pressure to prepare a green tea extract composition. The prepared green tea extract composition was concentrated 10 times at 60 ° C., and then diluted 10 times (0.1%), 100 times (0.01%) and 1000 times (0.001%), and used in the following experiment. In the following experiments, absorbance is the absorption wavelength of the color produced after color development.

Sample code Extraction solvent (% by weight) Comparative Example 2 A 100% purified water Comparative Example 3 B 100% ethanol Example 4 C 70% Ethanol + 30% Concentrated Water Example 5 D 100% concentrated water Example 6 E Electric dialysis

Experimental Example 3 Antioxidant Effect of Green Tea Extract Composition

(1) NBT assay

In a 300 mL phosphate buffer (pH 7.8), 1.6 mL of Triton X-100, 2.9 mg of EDTA (ethylenediamine tetraacetic acid), 1 mg of NBT (nitro-blue-tetrazolium), 5.4 mg of hypoxanthine Substrate / reactive solutions were prepared. 12 μL of xanthine oxidase was dissolved in 10 mL of 300 mM phosphate buffer (pH 7.7) to prepare an enzymatic solution. 1.0 mL of buffer was added to a 24-well plate, 0.5 mL of substrate / reaction solution was added, and 100 μL of each diluted sample was treated. In the control group, only the solvent was treated, and then 0.5 mL of an enzymatic solution was added thereto, followed by reaction at 37 ° C. for 30 minutes, and the absorbance was measured at 540 nm. The scavenging efficiency (inhibition rate of NBT reduction,%) of super-oxide anion measured by the reduction of NBT in the xanthine-xanthine oxidase system is shown in FIG. 1. When the substrate is treated with an enzyme, oxidation occurs and color develops. As oxidation progresses, color development becomes stronger and the absorbance increases, and thus, the antioxidant effect of the green tea extract can be determined from the absorbance of the enzyme solution.

(2) DPPH radical scavenging ability

Distilled water: methanol was mixed at a volume ratio of 4: 6 to make a stock solution, and DPPH (diphenyl-picry-hydrazyl, sigma) was dissolved, and the absorbance was about 0.6 at 513 nm. 2 mL of the DPPH solution and 1 mL of the diluted sample were added to a 24-well plate, reacted at room temperature for about 1 hour, and the absorbance was measured at 513 nm. Free radical scavenging activity (anti-hydroxyl radical rate,%) for DPPH was measured and shown in FIG. Since the color of the solution becomes darker when the DPPH is oxidized, the antioxidant effect of the green tea extract can be determined from the absorbance of the solution.

Experimental Example 4 Whitening Effect of Green Tea Extract Composition

(1) L-DOPA oxidation inhibition

900 μL of the diluted sample was added to a 24-well plate, and then treated with 50 μL of 2000 u / mL mushroom tyrosinase (dissolved in 0.1 M phosphate buffer pH 6.5), and left at 37 ° C. for about 5 minutes. After reacting 50 μL of 10 mM L-DOPA (pH 7.0) at room temperature for 1 minute, the absorbance was measured at 490 nm. L-DOPA auto-oxidation inhibitory effect of each sample was measured and the results are shown in FIG. 3. By the action of tyrosinase (tyrosinase), tyrosine (tyrosine) changes to a substance called L-DOPA, again tyrosinase acts to automatically oxidize L-DOPA, after which melanin pigment is produced to change the skin color . Therefore, the whitening effect can be confirmed by measuring the inhibition rate of L-DOPA automatic oxidation and the inhibition rate of tyrosinase activity. In this experiment, browning which occurs when L-DOPA is treated with tyrosinase is measured as absorbance at 490 nm.

(2) Inhibiting Tyrosinase Activity

100 μL of 1M phosphate buffer (pH 6.5) and 100 μL of 15 mM tyrosine solution were added, followed by 100 μL of diluted sample by concentration. 100 μL and 600 μL purified water of 2,000 U / mL mushroom tyrosinase were added thereto, and enzyme activity (enzyme kinetic) at 25 ° C. and absorbance at 475 nm were measured to determine the tyrosinase activity inhibition rate.

[ Experimental Example  5] Anti-wrinkle Effect of Green Tea Extract Composition

To a 24-well plate, add 100 μL of 5 mg azocoll (azocoll, azo dye impregnated collagen, Sigma, A4341), 500 mM Tris-HCl buffer (pH 7.5) and 5 mM Ca (C ₂ H ₃ O ₂ ) ₂ solution. After treating 900 μL of the diluted sample for 10 minutes and reacting at 37 ° C. for 10 minutes, 100 μL of collagenase was treated and reacted at 37 ° C., and then the absorbance was measured at 530 nm. The measured collagenase inhibitory effect is shown in FIG. 4. When collagen is broken down by collagenase, wrinkles are formed on the skin. When collagen (azocol) to which color is added is treated with collagenase, the higher the collagenase activity, the darker the color of the azocol solution. Therefore, the effect of inhibiting collagenase activity of green tea extract can be confirmed from the color of the collagen (azocol) solution.

[ Experimental Example  6] Anti-inflammatory Effect of Green Tea Extract Composition

To the 24-well plate, 0.5 mL of diluted sample and substrate solution (50 μL of Linoleic acid + 50 μL of Tween 50 + 50 mL of 0.1 M Tris-HCl (pH 7.0) purified water) were added, followed by 0.5 mL of enzyme (10 μL Lipoxygenase (1,000,000 units / mL) and 0.3 mL of 0.3M Tris-HCl buffer) were added to react. After the enzymatic reaction, 0.25 mL of saturated KI solution (KI 35g + purified water 25mL) and 0.25mL of 1% starch were added thereto, followed by color development, and the absorbance was measured at 540 nm. The measured lipoxigenase inhibition rate is shown in FIG. 5. Treatment with a linoleic acid with an enzyme involved in inflammation (lipoxigenase) produces peroxides, where KI treatment releases I, and starch treatment reacts with and develops I. In other words, if the activity of the enzyme is high, the formation of peroxide increases, a large amount of I is released, and dark color appears. Therefore, the inhibition rate of lipoxigenase of green tea extract can be confirmed from the degree of occurrence of the enzyme reaction.

From the Experimental Examples 3 to 6 (FIGS. 1 to 5), the green tea extract composition according to the present invention is effectively extracted green tea, compared with the conventional green tea extract composition, antioxidant effect, whitening effect, anti-wrinkle effect, The anti-inflammatory effect is excellent.

1 is a graph showing the removal efficiency of super oxide anion in the xanthine-xanthine oxidase system when the green tea extract composition according to the Examples and Comparative Examples of the present invention is applied.

2 is a graph showing the free radical removal activity when the green tea extract composition according to the Examples and Comparative Examples of the present invention is applied.

3 is a graph showing the L-DOPA automatic antioxidant effect when the green tea extract composition according to the Examples and Comparative Examples of the present invention is applied.

4 is a graph showing the collagenase inhibitory effect when the green tea extract composition according to the Examples and Comparative Examples of the present invention is applied.

5 is a graph showing the inhibition rate of lipoxigenase when the green tea extract composition according to the examples and comparative examples of the present invention is applied.

Claims (4)

5-30% by weight of green tea extract; And 70 to 95% by weight of the extraction solvent containing the deep sea water Green tea extract composition comprising. The green tea extract composition according to claim 1, wherein the extraction solvent has a hardness of 100 to 13,000. The green tea extract composition according to claim 1, wherein the extraction solvent comprises 5 to 70% by weight of a solvent selected from the group consisting of purified water, an organic solvent and a mixture thereof and the remaining deep sea water. The green tea extract composition according to claim 1, wherein the green tea extract comprises a compound selected from the group consisting of catechin, tannin, free amino acid, free sugar, saponin, and organic acid.

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