KR20080079162A - Assorted feedstuff composition containing chia seed or its extract, method of raising chicken using the same, and chicken meat acquired thereby - Google Patents

Abstract

Assorted feed containing Chia seed or its extract, a method for raising chicken by using the assorted feed, and the chicken are provided to reinforce the digestive organs and circulatory system of chicken and improve its liver function by allowing the chicken to take the dietary fiber and Ћ-3 fatty acid contained in the Chia seed. A poultry feed composition comprises one of Chia seed, Chia cake, and their mixture as a substitute for antibiotics. The poultry feed composition contains 0.84~29wt% of Chia seed, based on the total weight of the composition. The poultry feed composition containing Chia cake and a source of Ћ-3 fatty acid is enriched with dietary fiber at a level of 0.23~8.1wt%(based on the total weight of the composition) and Ћ-3 fatty acid at a level of 40~75wt%(based on the total weight of the dietary fiber). A method for raising chicken comprises supplying the assorted feed to chicken for 5days or more while separately supplying drinking water, wherein the assorted feed contains 1~20g of the Chia seed in feed intake per day. The chicken is low in antibiotics residues and has an improved meat quality.

Description

Assorted Feedstuff Composition Containing Chia Seed or its Extract, Method of Raising Chicken Using the Same, and Chicken Meat Acquired hence}

1 is a graph of the molecular weight pattern of dietary fiber components contained in chia seeds identified through gel osmochromatography from the powder of chia seeds.

Figure 2 is a table showing the molecular weight of the various dietary fiber components contained in the chia seeds identified through gel osmochromatography from the powder of chia seeds.

3 is a material separation flowchart for separating active ingredients of chia seed.

Figure 4 is a photograph showing the results of the MRSA target experiment to determine the antibiotic resistance inhibitory effect of chia seed active ingredient.

FIG. 5 is a graph analyzing the antioxidant effect of each chia seed fraction having resistance from livestock diseases by DPPH method.

The present invention relates to a feed composition and a chicken breeding method, and more specifically, to a feed composition that can promote growth while increasing the immunity of chickens by adding natural ingredients collected from nature without using antibiotics, and such feeds The present invention relates to a chicken breeding method capable of producing a well-being chicken product which can induce growth and prevent various adult diseases while enhancing the immunity of broilers.

Increasing national income and changing dietary conditions resulting from improved living standards have led to an increase in meat consumption.As a result of the westernization of the national diet, the consumption of livestock products has increased due to the growing tendency of the national diet and the objection to the intake of animal fats due to the consumption of animal fats. Is being raised. In particular, as the public's interest in health has increased and the 5-day work has progressed to a well-being society, it is cheaper and contains high-quality protein among other meat products for the purpose of preventing obesity and adult diseases and maintaining a healthy life. The demand for chicken is constantly growing. However, chickens are very likely to be infected during the breeding process due to unsanitary housing conditions.In particular, in factory farms where large-scale densities are raised, some of the infections spread rapidly to the whole house as well as to the neighboring houses. Breeding chickens are always at risk of mass mortality. In particular, in recent years, large-scale broiler breeders have been selling large-scale white semi broilers, which are produced by breeding breeding hens and broiler chickens, in order to lower the cost of broilers. Poultry farming has become a serious social problem as poultry farmers are concerned about side effects from vaccines and avoid vaccination against diseases such as avian influenza virus, Salmonella pullorum and Newcastle. The disease problem of chickens is not limited to the above-mentioned white rice, but can be common to chickens of other seeds. Even in the case of native chickens or chickens, which are known to be resistant to diseases, the same problem is caused by the breeding environment. It exists. In addition, as the public's awareness of food in the well-being society increases, the problem of chicken hygiene is regarded as an important health problem.

In general, feed for poultry is mainly made from grains such as barley, corn, sorghum and wheat, by-products such as by-products of wheat flour, soybean meal, and minerals. Is formulating feed with significant amounts of antibiotics to inhibit or kill the growth of pathogens. In fact, at the time of the present application, more than half of the domestic consumption of antibiotics for livestock is used as a feed additive, so it is known that antibiotics are used more for prevention than for treatment or protection. However, antibiotics added to the formulated feed prevent the disease of chickens and kill the harmful bacteria in the intestine, leading to smooth digestion and growth promotion. There is a fatal problem that accumulates in the body of the chicken, remains and is transferred to the consumer, which increases the human body's antibiotic resistance.

To prevent the accumulation of antibiotics in meat, the government has banned some antibiotics from being added to feeds, and even antibiotics allowed for use have been given a period of absence before the shipment of chickens. Antimicrobial residues in chickens shipped and Staphylococcus aureus (MRSA) or vancomycin resistant staphylococcus aureus (VRSA) or vancomycin resistant staphylococcus aureus (VRSA) are associated with the emergence of antibiotic resistance and the emergence of antibiotic-resistant plasmids. Antimicrobial abuse is regulated by setting standards for resistant bacteria that are resistant to even the most potent therapeutics for the drug. Feed companies also manufacture and sell feeds for the late broiler that do not contain antibiotics. However, many farms do not comply with these regulations, and either continue to abandon antibiotics or combine their own antibiotics. Therefore, there is an urgent need for additives that can effectively replace antibiotics added to blended feed.

Methods for eliminating or reducing the need for the addition of antibiotics in formulated feeds have been proposed prior to this application. For example, clay minerals such as kaolinite, zeolite, bentonite and vermiculite have been used for a long time to promote livestock development, improve digestibility and feed efficiency, control moisture of livestock and remove odors. (Pond et al. 1988, Hagedorn et al. 1990, Kovar et al. 1990), Son and Park (1997) proposed adding 0.3% elvan rock to broiler feed, and Kwon (1999) added far-infrared radiation to broiler pigs to improve the growth rate. Yang (1999) reported the addition of porous scoria to growing and hog feeds. In addition, germanium is known to have an anticancer effect by strengthening immunity, interferon production and stimulation, and protecting the living body from toxic substances and viruses.

  On the other hand, Korean Patent Publication No. 2002-34342 (name of the invention: a method of producing livestock feed using livestock and a method of breeding livestock) is a method for eliminating the problem of residual antibiotics in livestock products, after fermentation of sawdust and heat distillation. It is proposed to extract the active ingredient and add it to the livestock feed for poultry. Korean Patent No. 2004-104075 (name of the invention: a combination feed composition and a method of manufacturing the same) is used to increase the immunity of chickens without using any antibiotics, in the form of powdery loess, gallnet, and redwood. A herbal feed composition consisting of Wood, Turmerio, Clove, and Amud Cork Tree, and an additive formulated with pine needle powder, are added to a grain feed, and a preparation method thereof. In addition, Patent No. 572524 (name of the invention: antimicrobial feed additive) suggests the use of antibiotic antibacterial vesicle lipids produced by spawn as a feed additive for replacing antibiotics. Patent No. 643596 (name of the invention: a feed additive and a feed containing the same) is to increase the immunity of the livestock, molokia and honeysuckle having various physiological activities such as antioxidant, anticancer, antibacterial, antiviral, detoxification, and Feed additives containing ethanol, a byproduct of brewing, have been proposed. In addition, livestock feed containing ammonium molybdate (registered patent No. 5702), live feed containing oligosaccharides (registered patent No. 93674), low cholesterol cholesterol feed additive (registered patent No. 58297), and olefin-based fatty acids from pine oil Manufacturing method of the concentrate (patent application 1994-19344), production method of broiler containing broiler as feed containing feather powder and pyridoxine and chicken production produced by the above method (Patent No. 647082), livestock for antibiotic replacement Feed composition (registered patent No. 343367), herbal feed additives for broilers and its manufacturing method (registered patent No. 495605), animal feed compositions containing persimmon extracts (registered patent No. 485057) have been proposed. .

However, according to the above methods, since the raw materials used as the feed additives are not complicated or easily obtained in large quantities, it is difficult to mix and manufacture the feed additives and the feed composition on a large scale. .

On the other hand, a variety of cooking methods for chickens have been developed from a long time ago to the present, and the chicken cooking methods are unique to our country. As it is well known, Samgyetang is a popular food for nutritional supplementation by putting glutinous rice and ginseng, etc., inside chicken, and boiling ingredients such as garlic, Astragalus and jujube. Chick chicken is used, and a variety of samgyetang is also produced recently.

The chicken broiler for Samgyetang is also used for large-scale densely rearing in factory houses, and broiler feed for sale by feed companies is widely used in the breeding process. In general, broiler feeds sold by feed companies are produced for the early broiler, broiler, and late broiler feeds. In the early broiler and broiler feeds, the minerals such as raw ash, calcium, and phosphorus are fortified. And the digestive tract and skeletal formation is made smoothly, and in the case of late broiler feed is enhanced nutrients, such as crude protein, crude fat, crude fiber, so that the weight gain of chicken is made quickly and is known to improve the meat quality.

By the way, the chicken chicken for Samgyetang has a problem of excessively less lean meat content than the skeleton size. This is based on a program developed in developed countries, which is commonly used in poultry farms. In developed countries, chickens tend to reach 2 kilograms (Kg) of livestock, because consumers prefer breast meat. Breeding and broiler feed program is premised on this breeding, while in Korea, chicken for chickens are raised only to reach 1.5 kg (Kg) of live weight, especially for chicken broiler chicken 900 to 1,000 grams Since it is only until the weight is reached, it is estimated that the period for ingesting the late broiler feed is absolutely insufficient or that it is shipped without eating the late broiler feed at all. Considering this point, it is conceivable to feed the chicks early in broiler feed, but it is not preferable because the immune and digestive organs and skeletal formation may not be smooth.

Therefore, for the chicken broiler for Samgyetang, the lean meat content may be increased by feeding the early broiler or broiler broiler feed, but not the late broiler feed, so that the immune and digestive organs and the skeleton can be smoothly formed. What can be done is required.

The present invention is to solve such a problem, to provide a compound feed that can increase the antimicrobial and disease immunity of poultry by adding a material that can be harvested in a large amount in nature as an antibiotic.

In addition, another object of the present invention is to provide a method for raising chickens by such a feed compound.

In addition, the present invention is obtained by such a breeding method, it is another technical problem to provide a well-being chicken poultry with a small amount of antibiotic residue and improved meat quality.

In general, most chicken diseases are caused by digestive or respiratory infections.

Diarrhea caused by pathogenic E. coli, such as Salmonella, is prevalent in young chicks. Chickens with diarrhea, once treated, have a significant economic impact on their poultry farms due to their slow growth rate. Escherichia coli, which causes diarrhea, is infected by oral growth, attaches to the small intestine mucosa, proliferates, and produces toxins, which cause diarrhea by changing the osmotic pressure in the intestine. Chickens infected with E. coli and diarrhea show dehydration due to water loss and, in severe cases, die. Although vaccines are sometimes used to prevent E. coli diarrhea, the most widely used method is to feed a blend of antibiotics added to the main feed as shown above.

On the other hand, when looking at the breeding environment of chickens, due to the emissary temperature and ventilation is poor, and often poor management, this environment acts as a stress factor for chickens tend to catch a cold. In particular, infectious bronchitis or avian influenza caused by these environmental factors is a worldwide problem. Once symptoms of bird flu occur, 75-100% of chickens can die and spread rapidly to all nearby poultry farms. Because of this, it is widely known to kill all chickens in the area.

The present invention is to add chia seeds or chia seed extract to poultry feed to strengthen the dietary fiber and omega-3 fatty acids in the feed composition, thereby strengthening the digestive organs of chicken by the action of dietary fiber, blood vessels by the action of omega-3 fatty acids By strengthening the circulatory function of the back and improve the respiratory and liver function to increase the immunity.

Looking at the characteristics and the efficacy of the chia seed used as a feed additive according to the present invention. The tooth (Salvia hispanica or Salvia columbariae) is a species of saruvia belonging to the genus Perilla Frutescens and Mint, which is a yearly subtropical plant (Hickman, James C. The Jepson Manual Higher Plants of California.University of California Press) , Berkeley Los Angeles London. (1993), USDA Natural resources Conservation Service. [Web application] .2006), although native to Mexico, are grown for commercial use in the central highlands of Argentina, Bolivia, Ecuador and Guatemala. Chia Seed has been used as a traditional food source of durability, fitness, health and beauty in Latin America, such as Mexico for over 1000 years, and has traditionally been consumed in Mexico, the Southwestern United States, and South America, but is widely known in Asia and Europe. Not in condition. Chia seed is gelatinized when it is put in water, and it expands 10 times. It also expands in the digestive system of the body, so it is used as a diet food because it sustains satiety by ingestion. These chia seeds contain 28% more fiber than lettuce, spinach and agar. In addition, chia seeds are about 32% vegetable fat, more than 60% of which is high in linolenic acid, such as omega-3 fatty acids. This omega-3 fatty acid content is much higher than that of other sources of omega-3 fatty acids, fish and flax. In addition, chia seed is rich in 18 essential and non-essential amino acids such as glutenic, diary, aspartic, leucine, valine, and alanine, and contains no calcium, calcium, phosphorus, magnesium, potassium, It contains abundant minerals such as iron, zinc and copper, and contains natural antioxidants. In addition, polysaccharides corresponding to dietary fiber in chia seeds have molecular weights of 74674, 21722, 8066, 2038, and 1050. These low- and medium-molecular weight mixtures are composed of porous fiber-fiber shells to support 5-40 times more water. If it is left for 2 to 30 minutes, it becomes a gel state, but the composition of the gel contains several polysaccharides (see FIGS. 1 and 2).

According to one aspect of the present invention, chia seeds are added to the poultry feed as it is, or the chia seeds are roasted or heated by heating the surface of the seed by other methods, or steamed and dried and added to the feed. In the following description and claims, it should be noted that the term 'chia seeds' is used to include chia seeds harvested from nature, as well as chia seeds or steamed chia seeds that have been surface treated by roasting or otherwise. do.

As a source of dietary fiber, the oil extracted from the teeth may be added to the feed instead of the toothpaste, ie toothpaste, with other materials such as perilla, basil, mushrooms and flax. Among these excipients, for example, perilla may be used in the range of 10 to 50% by weight, basil (10 to 50% by weight), mushrooms to 10 to 50% by weight, flax 10 to 50% by weight. Therefore, in the following description and claims, the term 'tooth foil' refers to the tooth oil foil remaining after extracting the oil, as well as the powdered powder by mixing an excipient in such tooth oil foil, and the same as the tooth oil foil or powdered above. It should be noted that it is used to mean that one toothpaste is included. On the other hand, as a source of omega-3 fatty acids, tooth oil, fish oil, perilla and the like may additionally be used. In the case of adding tooth foil or a mixture of tooth foil and other materials to the feed, the dietary fiber content and the omega-3 fatty acid content which are fortified in the feed are preferably set to be the same as in the case of adding chia seeds.

Such feed compositions may be supplied to farms in a blended state at large and business feed mills, and may be seeded and / or chopped in conventional blended feeds that are mixed with other sweet feed ingredients at each farm level. Other additives according to the present invention may be used in combination.

Specifically, the feed composition for poultry breeding according to the present invention contains any one of chia seeds, tooth foil, and combinations thereof to enhance dietary fiber and omega-3 fatty acids. When chia seeds themselves are used as a source of dietary fiber and omega-3 fatty acids, chia seeds are added at a rate of 0.84-29% by weight of the total feed composition. When chia seeds or the like are added to the feed instead of or with chia seeds, dietary fiber may be enriched by 0.23 to 8.1% by weight based on the total weight of the composition and 40 to 75% of the dietary fiber reinforcement by omega-3 fatty acids. To this end, it is desirable to add toothpaste and omega-3 fatty acid sources. Here, as the omega-3 fatty acid source, any one or combination of perilla, chia oil, flax seed and flax extract can be used. Here, perilla is 10-50% by weight, basil (basil) 10-50% by weight, mushrooms may be used in the range of 10-50% by weight, flax 10-50% by weight, a plurality of materials are added in combination Can be.

More generally, the feed composition of the present invention contains chia seeds, chia foil, or a combination thereof in a basic feed comprising all or a portion of corn, soybean meal, molasses, sun salt, vitamin mixture, mineral mixture, wherein the chia seed is 0.5 Wt% to 50% by weight may be added, and 0.5% to 50% by weight of chia seed foil may also be added.

In breeding chickens according to the present invention, a feed containing any one of chia seeds, tooth foils, and combinations thereof may be fed to a chicken immediately before being shipped for 5 days to strengthen dietary fiber and omega-3 fatty acids. By supplying drinking water separately, the chicken's immunity is improved and the chicken's quality is improved. At this time, chia seed or tooth foil is added to the feed as an antibiotic substitute. If chia seeds are added directly to the feed, chia seeds are added to contain 1 to 20 grams of the daily intake of the feed. Such feed compositions may be provided for example during the period for which antibiotics are prohibited by law. Depending on the feed amount, the chia seed weight may be increased or the chia seed component ratio may be gradually decreased by keeping the chia seed weight constant.

More specifically, in breeding chicken chicken for samgyetang having a live weight of 900 to 1,000 grams or less at the time of shipment according to the present invention, when chia seeds are directly added to the feed, the chia seeds are 1 to 10 grams in the daily feed intake. It is preferable to contain. In addition, it is preferable to feed such a feed from 7 days of age or later.

In the case of breeding chick-chicken as described above, if tooth paste is added to the feed instead of chia seeds, the dietary fiber is strengthened by 0.23 to 8.1% by weight based on the total weight of the feed composition, and 40 to 75% of the dietary fiber reinforcement amount is Omega-3 It is desirable to feed the feed composition containing toothpaste and omega-3 fatty acid sources so that the fatty acids are fortified. Here, as the omega-3 fatty acid source, any one or combination of perilla, chia oil, flax seed, flax extract, fish oil can be used. Here, perilla is 10 to 50% by weight, basil (basil) is 10 to 50% by weight, mushrooms may be used in the range of 10 to 50% by weight, flax 10 to 50% by weight. In addition, it is preferable to feed such a feed from 7 days of age or later.

On the other hand, the chicken breeding method according to the present invention can also be used to breed chickens for the late broiler chicken just before shipping to 1,500 to 1600 grams. In breeding such chickens, when chia seeds are added directly to the feed, it is preferable that the chia seeds are contained in 3 to 20 grams in the daily feed intake. In breeding such chickens, tooth foil may be added to the feed instead of chia seeds. Even in this case, it contains a dental food and omega-3 fatty acid source so that the dietary fiber is reinforced by 0.56 to 6.4% by weight relative to the total weight of the feed composition and the omega-3 fatty acid is reinforced by 40 to 75% of the dietary fiber fortification amount. Feed compositions can be fed.

Chicken breeding method according to the present invention can be generally applied irrespective of the seed, such as general broiler, white rice, chicken, metered chicken. In addition, the chickens raised in the above-described method has a small amount of antibiotic residue, a low fat content throughout the whole position, and has improved meat quality. In addition, Samgyetang or fried whole chicken and other chicken processed foods that are cooked using such chicken have little disgusting smell inherent in chicken cooking and significantly reduce fat components and provide unique flavors unlike conventional foods.

On the other hand, the addition ratio of the chia seeds or tooth foil as described above is exemplary and the present invention is not limited to the ratio, and may vary depending on the content of grains, rivers, oils, and other processed by-products that are the main ingredients of the feed. However, when the amount of chia seed is insignificant, the action of the dietary fiber and omega-3 fatty acid intended by the present invention may be insufficiently expressed, and when the amount of chia seed is added, the amount of dietary fiber may increase the economic cost as well as omega. -3 Despite the action of fatty acids there can be a problem that body weight can be reduced.

According to the present invention, when the chia seed extract such as chia seed or tooth foil is added to the feed, the omega-3 fatty acid, which is reinforced through chia seed, is diluted to be discharged to the outside of the cell and discharged through the blood vessel again. It inhibits the deposition of cholesterol in the blood vessels, thereby purifying the blood vessel walls, thereby helping to facilitate blood circulation. Accordingly, omega-3 fatty acids prevent cardiovascular diseases by enhancing circulatory functions such as blood vessels. In addition, omega-3 fatty acids will strengthen the lungs of chickens to prevent respiratory diseases, improve liver function and strengthen the skeleton to increase immunity. In addition, omega-3 fatty acids reinforced with chia seeds will also compensate for weight loss due to dietary fiber enhancement by supplementing calories and nutrients. In particular, in relation to chick chickens, omega-3 fatty acids provide enough calories and nutrition while feeding the early broiler or broiler feed, not the late broiler feed, so that the immune and digestive organs and skeletal formation can be facilitated. By doing so, you can improve chicken quality.

On the other hand, dietary fiber reinforced by chia seeds, such as water retention is high water retention ability to increase the volume of stool by 20-30% and dilute to function to prevent constipation. Dietary fiber adsorbs waste products such as heavy metals, bacteria, toxins, environmental hormones, residual pesticides and antibiotics, and carcinogens while talking to the digestive organs, helping to prevent contact with excretory membranes and excrete. Prevent cancer and colon polyps. In addition, dietary fiber helps to increase sugar metabolism slowly by reducing the contact area between intestinal and food to prevent the sugar from spreading rapidly into the blood, and improve the intestinal bacterial layer to improve the diarrheal and carcinogens caused by pathogenic bacteria. By suppressing production, helping beneficial bacteria such as bifidus bacteria predominate, and reducing the intestinal transit time of the stool, shortens the time for carcinogens to come into contact with colon cells and lowers the concentration of ammonia, one of the carcinogens, to reduce chicken immunity. Will increase. In addition, the dietary fiber itself is a food waste, which is the material of the stool and is excreted by the contents of the large intestine, and thus cleans the intestine and increases the weight of the stool to prevent stool in the digestive tract such as the intestine. As such, dietary fiber enhances the digestive system of chickens in various aspects to prevent chicken digestive diseases and increase immunity. In addition, dietary fiber contributes to the strengthening of the circulatory system and prevention of various cardiovascular diseases through the adsorption and excretion of cholesterol and triglycerides.

As such, when chia seeds or chia seeds extracts such as chia seeds are added to the feed, dietary fiber and omega-3 fatty acids, which are reinforced through chia seeds, are discharged to the outside of cells, blood vessels and digestive organs, and circulatory and digestive organs And by enhancing the liver function, the chicken's immunity will be increased.

EXAMPLE

Hereinafter, preferred embodiments of the present invention will be described in more detail.

Experimental Example 1

As described in Example 4 below, the inventors pay attention to the remarkably high shelf life of the chicken produced by the present invention, the antioxidant of the chia seed extract in order to confirm whether the chisid of the present invention has an antimicrobial effect or an antioxidant activity The action and antimicrobial effect were measured. First, 2,500 g of chia seeds were ground with a blender, and 10,000 ml of Methanol was added thereto, followed by concentration under reduced pressure at 60 ° C. for 24 hours, followed by MeOH frc. About 450 g was recovered and extracted twice with shaking with 5,000 ml of n-Hexane at room temperature for 24 hours. The hexane fractions were filtered through 0.4 μm filter paper and concentrated under reduced pressure at 35 ° C. to obtain 25 g of hexane fractions. In the same manner as above in 250g silica gel filled column chromatography (45mm x 900mm) and eluted with a chloroformol / methanol mobile phase chloroform (CHCl3) frc. 175 g eluted with 2000 ml of ethyl acetate to EtOAc frc. 64 g were recovered, and each was recovered by eluting with butanol (BuOH frc. 27 g), water (H 20 frc. 148 g), and Ether (Et 2 O frc. 11 g) (see FIG. 3).

Experimental Example 2

end. Antimicrobial Effect Test on Fractions of Chia Seed

Each of the chia seed fractions recovered in Experimental Example 1 at a concentration of 25 μg / ml, and then the solid medium DISC diffusion method (microbial and immunology subcommittee, experimental microbiology, university history) in order to test the validity of using the chia seed active ingredient as an antibiotic substitute. , p116,1998). First, the bacteria were purchased from the microbial spawn association, MRSA Staphylococcus aureus and Salmonella, and then suspended in distilled water with platinum, and then plated on a solid serum agar medium and incubated at 25 ° C for 24 hours. Here, each fraction of watmann paper disc (diameter 8 mm) was dropped on the disc to 20 μl at 25 μg / ml concentration and incubated at 25 ° C. for 24 hours. 25 μg / ml of ampicillin was used as a control antibiotic.

I. Experiment result

Table 1 shows the antimicrobial effect of each fraction extract on chia seed Salmonella. As shown in Table 1, the antimicrobial effect of Salmonella on broilers was the strongest antimicrobial effect of butanol and methanol fractions of chia seeds according to the disk diffusion method, about 28 ± 0.0mm and 24 ± 0.2mm, and the rest was moderate. It showed antimicrobial effect.

Fraction Zone Size of Inhibition (mm) Methanol fraction 24 ± 0.2 Water fraction 12 ± 1.0 Chloroform fraction 15 ± 0.5 Ethyl acetate fraction 16 ± 1.2 Hexane fraction 10 ± 2.5 Butanol fraction 28 ± 0.0 Ether fraction 16 ± 0.5

In addition, as shown in FIG. 4, as a result of examining the resistance inhibitory effect through MRSA Staphylococcus aureus, butanol fraction showed the strongest antimicrobial effect against MRSA Staphylococcus aureus, followed by ethyl acetate fraction and ether fraction. Implies that it contains potent antibacterial ingredients. Therefore, in view of the antimicrobial activity of the chia seeds, it is considered that the chia seeds are suitable for the use of antibiotics in livestock feed.

Experimental Example 3

end. Antioxidative Effect of Chia Seed Fraction

Antioxidant activity was assayed to detect useful active substances from fractions of chia seeds. The antioxidant activity assay using free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) was used. Methanol was added to the test tube, and the sample was added at a concentration of 25 μg / ml, 500 μl of the DPPH (0.3 mM) solution was added thereto, and reacted at room temperature for 30 minutes. The absorbance was measured at 517 nm, and vitamin C was used as a control. 5 Persons, Comparison of Methanol Extract Activity by Daily Varieties and Parts, Kor. J. Med. Crop Sci. 10, 212 (2002)).

I. Antioxidant Activity of Chia Seed Active Ingredients in Livestock Feed

Methanol (MeOH) fraction, hexane (n-Hexane) fraction, chloroform (CHCl3) fraction, ethyl acetate (EtOAc) fraction, butanol (BuOH) fraction, water (H20) fraction, Ether (Et2O) fraction The antioxidant activity of vitamin C was measured by DPPH method. The chief seeds of MeOH and BuOH were the best, about 90 ± 1 and 88.6 ± 1.5, followed by 72.6 ± 3.0, 67.3 ± 3.2, 21.0 ± 1.1, 17.6 ± 5.5, 7.3 ± 1.5, 34.3 ± 2.5 in the order of antioxidant activity, especially MeOH fraction and BuOH fraction exhibited significant antioxidant activity within the p <0.05 range, possibly absorbed in vivo when added as a livestock feed immune function It is anticipated that it will be able to enhance its ability to resist disease and disease (see Figure 5).

Example 1: Preparation of Combined Feed for Poultry Containing Chia Seed

In order to increase dietary fiber and omega-3 fatty acid doses simultaneously to increase the disease immunity of broilers and to prevent weight loss due to dietary fiber and to improve meat quality, feed containing chia seeds is prepared and broilers are located in the farm in Goesan, Chungcheongbuk-do. Breeding experiments were conducted.

First, in order to prepare a compound feed containing chia seeds, chia seeds are added to a conventional poultry feed, but 1 gram (g), 2 grams, 4 grams, 8 grams of Australian chia seeds in daily feed consumption per chicken. , 5 grams of chia seed compounded feed were prepared by adding 10 grams each.

Example 2: Breeding of chicken for whole chicken by compound feed

Using the feed prepared in Example 1, six chickens over 20 days of age were carried out for 15 days until they reached 1,500 to 1,600 grams of live weight which was shipped for whole chickens. For 5 out of 6 rats, chia seeds were mixed with 1 gram, 2 grams, 4 grams, 8 grams, and 10 grams of chia seeds in the daily feed consumption according to Example 1, respectively. Basic feed, that is, normal poultry feed, was used as feed. The test feed was fed in the form of powdered feed to be free vegetarian, and water was supplied separately so that it could be freely eaten.

On the other hand, in order to check the growth and meat changes of chickens according to the feeding period of the compound feed, five chickens were obtained after 30 days of age, and the breeding experiment was carried out for 5 days until 1,500 to 1,600 grams of live weight. Five chickens were fed the chia seed blended feed prepared in Example 1. The test feed was fed in the form of powdered feed to be free vegetarian, and water was supplied separately so that it could be freely eaten.

Example 3: Measurement of the weight change of broiler according to the feeding of the feed composition

As a result of measuring the live weight of the chickens raised as in Example 2, the weight gain of chickens fed the feed supplemented with dietary fiber by adding chia seeds to the feed did not show a significant difference from the control group without any dietary fiber. Weight gain was not significantly affected by the addition of chia seeds to the diet. However, chickens fed 10 grams of chia seed supplemented for 15 days were found to have a relatively low gain of 3%.

Chickens fed chia seed supplemented feed consumed about 3 ~ 10% more during this period and increased bowel movement more than 10% compared to the control. This fact suggests that dietary fiber in chia seeds promotes the intestinal excretion of chickens, nutrients that may be lacking in the process are recovered from omega-3 fatty acids in chia seeds, and additional deficiencies can be resolved by increasing feed intake. Inferred.

Example 4 Fatty Acids, Protein Degeneration, and Storage of Chicken

Fatty acid opacity or protein denaturation (VBN) was not quantitatively measured for chickens bred by the method of Example 2, but instead, the chickens were stored at room temperature instead of refrigerated for 7 days at 4 g of the chia seed. After keeping it up, the sensations were smelled. As a result, in the case of the control chicken, there was a bad disgusting smell due to protein denaturation, etc., but it was confirmed that the odor change in the chia seeds 4g addition broiler chicken than when removed from the refrigerator.

In connection with the above-mentioned increase in bacterial resistance to storage of chicken meat and Experimental Examples 1 to 3, when the chickens are raised using the feed containing chia seeds according to the present invention, the antimicrobial component contained in the chia seeds You can be sure that it will be digested and absorbed by the chicken and remain in the tissue. In addition, the action of this antimicrobial component is combined with the improvement of liver function by omega-3 fatty acid and dietary fiber, and even in the breeding process before slaughter, it can be expected that the immunity of chickens fed chia seeds is greatly increased.

Example 5: Component Analysis of Chicken

After the slaughter of chickens fed with the feed of 4 grams of chia seeds in the chickens bred by the method of Example 2 for 15 days, the cholesterol content and fatty acid content in the breast meat of the chicken were tested in the Food Code (2006) Measured according to. The cholesterol content measurement results are shown in Table 2 below, and the fatty acid content measurement results are shown in Table 3.

Metric Measurement result (mg / 100g) Chia Seed 4g Addition Control (base feed added) cholesterol 46.9 59.4

Chemical formula Fatty acid name Composition ratio (%) per 100 g of fat Chia Seed 4g Addition Control (Basic Feed Addition) C14: 0 Myristic acid 1.1 1.2 C14: 1 Myristoleic acid 0.3 0.3 C16: 0 Palmitic acid 25.3 25.9 C16: 1 Palmitoleic acid 6.6 7.1 C18: 0 Stearic acid 5.4 5.4 C18: 1 Oleic acid 38.0 39.1 C18: 2 Linoleic acid 19.2 17.8 C18: 3 (ω3) Linolenic acid 1.8 0.9 C20: 0 Arachidic acid 0.1 0.1 C20: 1 Gadoleic acid 0.3 0.4 C20: 2 Eicosadienoic acid 0.2 0.1 C20: 3 Eicosatrienoic acid 0.1 0.1 C20: 4 Arachidonic acid 0.5 0.5 C22: 6 (ω3) DHA 0.1 0.1 Etc 1.0 1.0 Sum 100.0

As shown above, cholesterol content in chicken was reduced by 21%, and omega-3 fatty acids in fatty acids were confirmed to double.

Example 6: sensory evaluation on meat taste

After slaughtering the chickens raised as above, the chickens were popped and subjected to a nine-point sensory evaluation on meat smell and meat quality for five sensory personnel.

Sensory evaluation results are shown in Table 4. Chickens reared by chia seeds added feed according to the present invention had better sensory performance than general chickens, that is, chickens fed conventional feeds, and significantly reduced meat smell and taste good. , The meat quality was confirmed to be soft and tender.

Sensory evaluation items Chia Seed Addition in Feed / Feeding Period Control (0% / 10 days) 1g / 15 days 2g / 15 days 4/15 days 6g / 15 days 10/15 days 1g / 5 days 2g / 5 days 4g / 5 days 6g / 5 days 10g / 5 days The smell of meat 6.0 6.4 6.8 8.6 8.8 9.0 6.2 6.6 7.7 8.4 8.6 Flesh 6.0 6.3 6.6 7.9 8.0 8.6 6.1 6.3 7.6 8.0 8.5

As such, poultry produced from chickens bred by the present invention has a lower cholesterol content, higher omega-3 fatty acid content, less disgusting meat smell, less tender meat, and does not rot easily than poultry produced by conventional methods. Characterized. In addition, in the breeding process, the compounded feed according to the present invention is expected to enhance disease digestibility by strengthening the digestive system of broilers.

Example 7 Broiler Chickens for Samgyetang by Blended Feed

Six chicks 10 days old were obtained, and the breeding experiment was conducted for 15 days until they reached 900 grams (g) of live weight which is shipped for Samgyetang. Five of the six animals were fed a chia seed compounded feed of 0.5 gram, 1 gram, 2 grams, 6 grams, and 8 grams of chia seeds in daily feed consumption, respectively, according to Example 1, and the other one was a control. Basic feed, that is, normal poultry feed, was used as feed. The test feed was fed in the form of powdered feed to be free vegetarian, and water was supplied separately so that it could be freely eaten.

On the other hand, in order to confirm the growth and quality changes of the chicks according to the feeding period of the compound feed, five chicks 15 days after the birth was obtained, and the experiment was carried out for 10 days until the weight of 900 grams. Five chickens were fed a chia seed compounded feed in which Example 1 was mixed with 0.5 gram, 1 gram, 2 grams, 6 grams, and 8 grams of chia seeds in the daily feed consumption. The test feed was fed in the form of powdered feed to be free vegetarian, and water was supplied separately so that it could be freely eaten.

Example 8 Measurement of Changes in Body Weight of Broiler According to Feeding of Feed Composition

As a result of measuring the live weight of the chicks reared as in Example 7, the weight gain of the chicks fed the diet supplemented with dietary fiber by adding chia seeds to the feed did not show any difference from the control group without the dietary fiber at all. In addition, the weight gain was not significantly affected by the addition of chia seeds to the feed. However, it was found that the weight gain of the chickens fed the compound feed containing 8 grams of chia seeds daily for 15 days was reduced by 4%.

Chick chickens fed chia seed supplemented foods consumed about 2 ~ 8% more during this period and increased defecation more than 3% compared to the control. This fact suggests that dietary fiber in chia seeds promotes the intestinal excretion of chickens, nutrients that may be lacking in the process are recovered from omega-3 fatty acids in chia seeds, and additional deficiencies can be resolved by increasing feed intake. Inferred.

Example 9: sensory evaluation on meat taste

The slaughtered chickens were slaughtered as above, followed by cooking samgyetang with poultry, and five sensory agents were subjected to a nine-point sensory evaluation on meat odor and meat quality.

Sensory evaluation results are shown in Table 5. The chickens of the chickens fed by the chia seeds added according to the present invention had excellent sensory performance compared to the general chickens, the meat smell was greatly reduced, the taste was good, and the amount of oil in the broth was reduced and the broth was reduced. It is clear that the flesh is soft and tender.

Sensory evaluation items Chia Seed Addition in Feed / Feeding Period Control (0% / 10 days) 0.5g / 15 days 1g / 15 days 2g / 15 days 6g / 15 days 8g / 15 days 0.5g / 10 days 1g / 10 days 2g / 10 days 6g / 10 days 8g / 10 days The smell of meat 6.0 6.4 6.9 8.3 9.0 9.0 6.3 6.9 7.9 8.3 8.6 Flesh 6.0 6.2 6.6 7.4 8.3 8.4 6.1 6.8 7.6 8.2 8.3

Example 10 Identification of Additional Features for Breeded Chickens and Their Meats

As a result of observing broilers bred by Examples 2 and 7, it was found that crests were red and clear in color compared to chickens bred in a conventional manner, and noticeably improved the gloss of feathers. In addition, in the process of slaughtering chickens bred by the present invention, compared to chickens bred in a conventional manner, the movements of chickens are large and the resistance is severe, and the slaughter is difficult. From this, it can be seen that the chickens bred by the present invention are much healthier and more vigorous than the chickens bred in the conventional manner, and as a result, the disease immunity is also increased.

In addition, immediately after the slaughter, and even after refrigeration, the chicken did not have the peculiar disgusting smell, and even the chicken feet or intestines had little smell. In addition, as mentioned above, even after several hours at room temperature, rather than refrigerated storage, the shelf life was high enough to be almost odorless, from which chickens bred by the present invention are bacteria despite the absence of antibiotics. It is further confirmed that resistance and disease immunity are greatly increased. On the other hand, when stored in the refrigerator again after exposure to room temperature, and then taken out of the refrigerating chamber for cooking two days later, the chickens fed the normal feed were damaged and could not be cooked, but the chickens fed the chia seed had no smell and the meat was There was no difference from immediately after the slaughter. Since there is no odor, the chicken according to the present invention did not need to use a separate perfume to remove the odor.

On the other hand, as seen in Example 6 and Example 9, the chicken broiled by the feed with chia seeds according to the present invention has been confirmed that the meat is soft and tender, in particular, in the case of common chicken breasts with wooden chopsticks While the chopsticks were stiff enough to break when trying to remove the meat, the chicken meat according to the present invention was light enough to be easily peeled off along the grains with wooden chopsticks.

In addition, the chickens bred by the present invention showed a generally thin skin and a significantly thin subcutaneous fat accumulated between the skin and the flesh. In particular, these low fat characteristics were prominent in thick areas of fat such as the neck, around the anus, and intestines. In addition, the chickens reared by the feed with chia seeds were found to have a white and clean shell color compared to the general chickens. In addition, when cooking the chicken soup using the chick chickens bred by the present invention, the oil in the broth is white, not yellow, and the amount of oil is also reduced, the soup is much clearer, unsaturated As the proportion of fatty acids increased, it was confirmed that the broth oil did not harden well even when cooled.

On the other hand, an experiment was conducted in which 10 g of chia seeds were fed per head for a group of 120 laying hens, which lay down about 80 eggs per day, resulting in lower egg production. As a result, in contrast to the phenomenon that the spawning rate gradually decreased when chia seeds were not fed, the spawning rate gradually increased when the chia seeds were fed as described above, and the egg laying rate was about 100 days after the start of the feeding. The increase in egg production rate is believed to contribute to preventing aging and increasing bone density by chia seeds activate the hormone secretion system of chickens.

Apart from this experiment, the same amount of chia seeds was fed to the eggs and the characteristics of the eggs were observed, indicating that the egg yolk had a high elasticity, a dark yellow color close to orange, and the egg shells were harder and fresher. Was done.

Example 11: Modified Example

The poultry feed described in Example 1 was described as the addition of chia seeds to a conventional feed as a source of dietary fiber and omega-3 fatty acids. Here, the chia seeds may be combined with the single feed ingredients such as cereals, rivers, oils, and other processed by-products from feed companies, or directly added after purchasing the conventional mixed feeds containing these single feed ingredients in livestock farms. It can also mix | blend.

If dietary fiber needs more reinforcement, mushrooms with high dietary fiber content, such as woody mushrooms, shiitake mushrooms, and oyster mushrooms, may be added, but by-products generated during mushroom packaging may be added in consideration of economic feasibility. have. In addition, basil (Ocymum basilicum L.) seeds may be added together with the chia seeds.

In the above-described embodiment, experiments were conducted in which 0.5 to 10 g of chia seed was added to the daily intake of the feed. In order to show the effect of the addition of chia seed, the chicken for chicken was more than 2 to 4 g and the chicken for samgyetang. It has been found that at least 1 g of chia seeds is added to the feed. In addition, a separate experiment was conducted with regard to the upper limit of the amount of chia seeds added. In the case of whole chickens, the addition of more than 15-20 g of chia seeds in the daily feed intake limits the improvement of meat quality, fat reduction and odor removal. On the other hand, weight loss was remarkable, and for chickens for Samgyetang, the weight loss was noticeable while the marginal utility was minimal when 8 ~ 10g of chisids were added to the daily feed intake. Therefore, in the case of whole chickens shipped at 1,500 to 1,600 grams of live weight, 3 to 20 g of chia seeds are added to the daily feed intake, and in the case of chicken chicken for samgyetang, which is shipped at 900 to 1,000 grams, 1 It was judged that it was desirable to add ˜10 g.

On the other hand, through the chicken breeding and sensory test for about 7 months, it was confirmed that it is preferable to feed chia seeds for a period of 5 days or more immediately before shipment. This need for feeding usually takes about 48 hours for unsaturated fatty acids, such as alpha-linolenic acid, to be converted into EPA in the body and about 96 hours, or 4 days, for conversion to DPA or DHA (Robert J. Pawlosky et al., Essential fatty acid metabolism in the feline: relationship between liver and brain production of long-chain polyunsaturated fatty acids , J Lipid Research 1994; 35 (11): 2032-2040).

In relation to the start of chia seeds, when diarrhea was fed to chicks before 7 days of age, there was a problem such as diarrhea. However, after 7 days of age, 1 g of chia seeds was added to the daily intake. It was confirmed that feeding chickens had no problem digesting them.

On the other hand, as mentioned above, oil can be extracted from the chia seed and the remaining tooth paste, ie, tooth foil, may be added to the feed instead of the chia seed, so as to reduce the feed cost burden caused by the chia seed material cost. In this case, other sources of fiber may also be used, such as perilla, basil or flax. As such, when using the tooth foil or the composition containing the tooth foil as an additive, it is preferable that a separate source of omega-3 fatty acid is added together so as to replenish nutrients and increase circulatory and respiratory disease resistance. Perilla, tooth oil, fish oil, etc. may be used as the omega-3 fatty acid, and chia seeds may be partially added.

Thus, in order to calculate the dietary fiber and omega-3 fatty acid fortification level when adding tooth foil or the like instead of or with chia seeds, the amount of chia seed addition which is considered suitable for breeding chicken chicken for samgyetang as described above. Dietary fiber and omega-3 fatty acid for the lower limit 1g and upper limit 10g is calculated by breeding age as follows.

Age Body weight (g) Feed amount (g / day) 1 g of chia seeds Add 10g chia seeds Tooth content (%) Dietary Fiber Reinforcement (%) Omega-3 Enhancement (%) Tooth content (%) Dietary Fiber Reinforcement (%) Omega-3 Enhancement (%) 7 155 34.5 2.90 0.81 0.56 28.99 8.12 5.57 15 394 65.7 1.52 0.43 0.29 15.22 4.26 2.92 25 903 110.7 0.90 0.25 0.17 9.03 2.53 1.67 27 1,032 119.6 0.84 0.23 0.16 8.36 2.34 1.61

In the above table, the feed according to age was used for the general broiler program proposed by Cargill for generalization. As shown in the table above, when 1-10 g of chia seeds is added to the feed, the content of chia seeds in the feed is 0.84 to 29% by weight, the dietary fiber content is about 0.23 to 8.12% by weight of the whole feed, Omega-3 fatty acids are fortified by 69% of dietary fiber fortification. Therefore, even when the toothpaste or the composition containing the toothpaste is added to the feed instead of chia seeds, the dietary fiber content is about 0.23 to 8.12% by weight of the total feed, 69% of the dietary fiber reinforcement, generally 40 It is desirable to enhance omega-3 fatty acids by -75%.

On the other hand, in order to calculate the dietary fiber and omega-3 fatty acid fortification level in the case of adding chia seeds instead of or with chia seeds in broiler broiler chickens, the lower limit of the amount of chia seed added to be considered suitable for broiler chickens 3g If the dietary fiber and omega-3 fatty acid for the upper limit of 20g is calculated by breeding age as follows.

Age Body weight (g) Feed amount (g / day) 1 g of chia seeds Add 10g chia seeds Tooth content (%) Dietary Fiber Reinforcement (%) Omega-3 Enhancement (%) Tooth content (%) Dietary Fiber Reinforcement (%) Omega-3 Enhancement (%) 20 619 87.6 2.90 0.81 0.56 28.99 8.12 5.57 34 1,525 145.3 2.06 0.58 0.40 13.76 3.85 2.64 35 1,599 149.2 2.01 0.56 0.39 13.40 3.75 2.57

As shown in the table above, when 3 to 20 g of chia seeds is added to the feed, the content of chia seeds in the feed is 2.01 to 22.83 wt%, the dietary fiber content is about 0.56 to 6.39 wt% of the total feed, Omega-3 fatty acids are fortified by 69% of dietary fiber fortification. Therefore, even when the tooth foil or the composition containing the tooth foil is added to the feed instead of chia seeds, the dietary fiber content is about 0.56-6.39% by weight of the total feed, 69% of the dietary fiber reinforcement, generally 40 It is desirable to enhance omega-3 fatty acids by -75%.

The above description is based on the case of feeding feed with chia seeds from approximately 20 days of age for chicken broiler chickens and 7 days of age for chicken chickens for samgyetang chicken. In this case, the start of the salary may be changed, of course, for example, chia seeds, etc. may be provided during the antibiotic administration prohibition period set by law.

On the other hand, in order to increase the disease immunity of the chicken in the present invention to add chia seed or tooth foil to the feed, charcoal powder or the like can be additionally added as a component for removing toxins and wastes in the body. In this case, it is expected that chicken's disease immunity can be further enhanced through adsorption and removal of toxins and waste products in the body by charcoal.

According to the present invention, a compound feed for poultry added with chia seeds or tooth foil may be utilized in the same manner as chickens for breeding not only chickens but also ducks and other poultry.

In addition, those skilled in the art will understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features. Therefore, the above-described embodiments are to be understood as illustrative in all respects and not as restrictive. The scope of the present invention is shown by the following claims rather than the detailed description, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the present invention. do.

As described above, according to the present invention, chia seeds or a mixture of extracts and other materials, which are materials that can be collected or grown in large quantities in nature, are used as feed additives instead of antibiotics, and chickens are used using the same. In view of the increased shelf life of poultry, the antimicrobial component of the chia seeds identified by the inventors can be convinced that during the rearing process, the antimicrobial component is absorbed by the chicken and remains in the poultry tissue. Combined with dietary fiber digestive and circulatory and liver function enhancements, the chicken's immunity is greatly increased without the addition of antibiotics.

In addition, in view of regenerating the spawning capacity of the broiler chickens, the chicken breeding method of the present invention has an effect of activating the hormone secretion system of chickens and preventing aging beyond simply raising the chicken's disease immunity.

Unlike native chickens, large-scale broilers are known to have a high economical rate of economical growth, but the disadvantage of low quality chicken meat, the present invention by adding chia seeds containing a large amount of dietary fiber and omega-3 fatty acids There is an advantage that can greatly improve the chicken quality of the dedicated broiler. That is, the chicken produced by the present invention has the advantage of not having the peculiar disgusting smell peculiar to the chicken, the meat is soft, the skin is thin, and the subcutaneous fat is remarkably thin. And the shelf life of the chicken is improved compared to the case of using antibiotics. In particular, for chicken chicken for chicken soup that is shipped early, the present invention allows the immune and digestive organs and skeletal formation to be made smoothly, while feeding nutrients for early broiler or broiler medium, not feed for late broiler feed Enough to make it is effective to increase the lean meat content and improve meat quality. On the other hand, the chicken breeding method of the present invention can be used not only for breeding broiler chickens, but also for breeding chickens of other seeds such as native chickens, improved native chickens, white rice, and the like.

Unlike the ruminants that generally convert unsaturated fatty acids to saturated fatty acids, the meat-improving effect is that in the case of chickens, the unsaturated fatty acids supplied through the feed can be transferred directly into the body tissues through the blood. The use of oil and fat for the production of economic animals, Korea Journal of Petrochemicals, 14:13, 1997). Therefore, when feeding the feed composition to the chicken, such as chia seeds containing unsaturated fatty acid as described above, the unsaturated fatty acid is accumulated in the chicken can be expected to increase the unsaturated fatty acid content and less saturated fatty acid and cholesterol content, the present invention Ingestion of chickens in humans prevents cardiovascular and cerebrovascular diseases, atopic dermatitis, osteoporosis and skin aging (Fan, YY and Chapkin, RS, The J. of Nurition, 128: 1411, 1998) You can expect.

As such, the tooth seed added to the feed composition according to the present invention significantly reduces mortality and prevents abuse of antibiotics through antibacterial, antioxidant, immune system, digestive, circulatory, respiratory, and immune system enhancement effects. It is possible to promote the health of the human body through indirect intake of omega-3 fatty acids and dietary fiber present in the chia seeds through the broiler.

Claims (23)

A poultry breeding feed composition containing any one of chia seeds, tooth foils, and combinations thereof as an antibiotic substitute to increase poultry immunity. The poultry breeding feed composition according to claim 1, which contains 0.84 to 29% by weight of chia seeds in the total composition. The toothpaste and omega-3 fatty acid source are contained so as to reinforce the dietary fiber by 0.23 to 8.1% by weight relative to the total weight of the composition and the omega-3 fatty acid by 40 to 75% of the dietary fiber reinforcement amount. Poultry breeding feed composition. 4. The feed composition of claim 3 wherein the omega-3 fatty acid source comprises perilla. The feed composition of claim 3 wherein the omega-3 fatty acid source comprises tooth oil. The feed composition of claim 3, wherein the omega-3 fatty acid source comprises any one or a combination of flax seeds and flax extract. Chicken breeding method to increase the immunity of chickens by feeding the feed containing any one of chia seeds, tooth foils, and combinations thereof as a substitute for antibiotics to increase the immunity of poultry, and by supplying drinking water separately to the chickens immediately before shipment . The chicken breeding method of Claim 7 which feeds the said feed more than 5 days. The chicken breeding method of claim 7, wherein the feed contains 1 to 20 grams of the chia seeds in a daily feed intake. The chicken breeding method according to any one of claims 7 to 9, wherein the feed is fed during an antibiotic administration prohibition period. 10. The chicken breeding method of claim 9, wherein the chia seed weight ratio is gradually reduced by maintaining a constant chia seed weight in the feed. The chicken breeding method of claim 9, wherein the feed contains 1 to 10 grams of the chia seeds in a daily feed intake, and the chickens to be shipped are chicken chickens for samgyetang which are shipped at 900 to 1,000 grams of live weight. The chicken breeding method of claim 10, wherein the feed is fed from 7 days of age or older. The method according to claim 7, wherein the dental fiber and omega-3 fatty acid source is contained so as to strengthen the dietary fiber by 0.23 to 8.1% by weight relative to the total weight of the feed and 40 to 75% of the dietary fiber reinforcement amount Feed the feed to the chicken breeding method of chicken chicken for Samgyetang, wherein the chicken is shipped to 900 to 1,000 grams of live weight. 15. The method of claim 14, wherein said omega-3 fatty acid source in said feed comprises tooth oil. 15. The method of claim 14, wherein said omega-3 fatty acid source in said feed comprises one or a combination of flax seeds and flax extract. The chicken breeding method of Claim 14 which feeds the said feed from 7 days old or after birth. The chicken breeding method according to claim 9, wherein the feed contains 3 to 20 grams of the chia seeds in a daily feed intake, and the chickens to be shipped are chicken broilers that are shipped at 1,500 to 1600 grams of live weight. The method according to claim 7, wherein the dental fiber and the omega-3 fatty acid source is contained so as to strengthen the dietary fiber by 0.56 to 6.4% by weight relative to the total weight of the feed and 40 to 75% of the dietary fiber fortification amount Feed the feed to the chicken breeding method of chicken broiler chicken, which is shipped to the 1,500 to 1600 grams of live chicken. 20. The method of claim 19, wherein said omega-3 fatty acid source in said feed comprises tooth oil. 20. The method of claim 19, wherein said omega-3 fatty acid source in said feed comprises one or a combination of flax seeds and flax extract. Chicken meat produced by the breeding method of claim 7, wherein the amount of antibiotic residue is small and the meat quality is improved. Chicken processed food made using the chicken produced by the breeding method of claim 7.

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