KR20080069414A - Product using micro-pulverized powder of cordyceps and method for preparing thereof - Google Patents

Abstract

A method of manufacturing a health supplementary food using ultra-fine powder of Cordyceps sinensis obtained is provided to maximize pharmacological activity such as antioxidant, anti-inflammatory and anti-cancer effect and increase unsaturated fatty acid. Ultra-fine powder of Cordyceps sinensis is mixed with lactose in a weight ratio of 1:2 and granulated. The ultra-fine powder of Cordyceps sinensis is obtained by the steps of: drying Cordyceps sinensis at 125deg.C to a moisture content of 20% in a far infrared ray dryer in a first step and to a moisture content of less than 5% and grinding to a particle size of less than 44 micrometers at grinding speed of 80 to 90m/s.

Description

Health supplement food using ultrafine powder of Cordyceps sinensis and its manufacturing method {Product using micro-pulverized powder of Cordyceps and method for preparing carrot}

FIG. 1 illustrates a change curve of water content (Wb) according to drying time for each drying temperature when a cordyceps is dried using a far-infrared dryer.

Figure 2 is a graph comparing the effect of the drying method (freeze drying, vacuum drying, far infrared) of Cordyceps sinensis on the effect on the antioxidant (DPPH).

Figure 3 shows the particle size distribution of each pulverization rate when grinding the Cordyceps sinensis using an ultra fine grinding machine.

Figure 4 is a photograph of the cordyceps ultrafine powder observed in TEM.

Figure 5 shows the solubility extracted for 30 minutes in water according to the solubility measurement temperature of Cordyceps sinensis by grinding (4 ℃, 50 ℃, 100 ℃).

Figure 6 is a graph showing the antioxidant effect of Cordyceps sinensis water extract by grinding.

Figure 7 shows the inhibitory effect of NO production and iNOS protein expression of Cordyceps ethanol extract (a) and water extract (b).

Figure 8 shows the fatty acid analysis according to the grinding method of Cordyceps sinensis.

Figure 9 shows Cordyceps fine powder, granules and tablet prototype.

10 illustrates a powder difference using Cordyceps sinensis.

The present invention relates to a health supplement food using ultrafine powder of Cordyceps sinensis and a method of manufacturing the same. More specifically, the present invention investigates the method of drying Cordyceps sinensis with high drying efficiency through lyophilization, vacuum drying or far-infrared drying, and ultrafine powdered Cordyceps sinensis dried above using a grinder to increase the pharmacological activity of the product. It is about manufacturing.

Cordyceps sinensis has been reported to have various pharmacological activities such as immunity enhancement, inflammation suppression and stamina enhancement, tuberculosis, jaundice, drug addiction detoxification, anticancer effect, etc. In particular, anti-inflammatory effects include "Cordycepin inhibits lipopolysaccharide-induced inflammation by the suppression of NF-kappaB through Akt and p38 inhibition in RAW 264.7 macrophage cells. Eur J Pharmacol . 2006 Vol. 545, pp. 92-199." And " Methanol extract of Cordyceps pruinosa inhibits in in vitro and in vivo inflammatory mediators by suppressing NF-kappaB activation. Toxicol Appl Pharmacol . 2003, Vol 1. pp1-8 ". The anticancer effect is" In vitro Antitumor Activity of Ergosterol Peroxide Isolated from Cordyceps militaris on Cancer Cell Lines from Korean Patients. The Korean Journal of Mycology 2001, Vol. 29, pp61-66. Antioxidant effects include "Anti-inflammatory and related pharmacological activities of cultured mycelia and fruiting bodies of Cordyceps militaris Journal of Ethnopharmacology 2005, Vol 96, pp 555-561 "and" Comparison of protective effects between cultured Cordyceps militaris and natural Cordyceps sinensis against oxidative damage. Agric Food Chem. 2006, Vol, 19. pp. 132-138 ".

Although the mushroom has the broadest pharmacological effect among the mushrooms, there is still a lack of evaluation on the pharmacological physiological activity and application of the high value-added products of Cordyceps sinensis. Conventional processed foods using Cordyceps sinensis are packaged in a tea bag or manufactured in a pill to commercialize, but the situation is required for manufacturing technology to further enhance Cordyceps sinensis products by granulating or tableting Cordyceps sinensis. Patents related to the processing of Cordyceps Sinensis include Korean Patent No. 0412332 "Health Supplement Tea using Militaris Cordyceps Sinensis and its manufacturing method".

In order to manufacture Cordyceps sinensis processed products, lyophilizer is used to control Cordyceps indigenous nutrition, color, and aroma, but it is a big burden for farmers. have.

In addition, when processing with a grinder such as a roller mill, a hammer mill, or a pin crusher, more than 95% of the energy required for crushing is generated by frictional heat, and less than 5% is required for crushing. (60 ~ 120 ℃), the grinding ability and grinding efficiency is lower when crushing fibrous or high fat raw materials that are not easy to crush. Most of them are lost and cause problems such as contamination of powder by excessive friction.

Therefore, the object of the present invention is excellent in the drying efficiency in the Cordyceps herb drying process, while maintaining the pharmacological effect of Cordyceps maximally, while having a unique fragrance and color of Cordyceps in the production of powdered tea, there is a problem of odor of the existing Cordyceps To provide a drying method that can remove the method and granules or tablets of Cordyceps sinensis through this.

Another object of the present invention to provide a functional product using the Cordyceps Sinensis powder.

The object of the present invention is to investigate the drying efficiency by drying the Cordyceps sinensis using far-infrared drying, and compare the physical properties with conventional products through particle size analysis, solubility and fatty acid analysis of the powder after ultrafine grinding of dried Cordyceps sinensis, Cordyceps sinensis Investigation of the antioxidant, anti-inflammatory and anti-cancer effects of pharmacological activity was achieved by manufacturing a functional application product superior to conventional products.

The present invention is a drying step of Cordyceps sinensis through far-infrared drying; Ultrafine grinding step of dried Cordyceps sinensis; Investigating the properties of the ground cordyceps; And it is composed of a commercialization step using the cordyceps ultra fine grinding.

The present invention is characterized by providing a method for preparing a dietary supplement using Cordyceps Sinensis with enhanced pharmacological activity including antioxidant, anti-inflammatory or anticancer activity, comprising the following steps:

Firstly drying the Cordyceps sinensis in a far-infrared dryer at 125 ° C. to a water content of 20%, and drying the Cordyceps sinensis dried at 80 ° C. to a water content of 5% or less,

Grinding the dried Cordyceps sinensis into a fine powder having a particle size of 44 μm or less by crushing at a raw material supply amount of 10 kg / hr and a grinding speed of 80 to 90 m / s, and

Mixing the cordyceps ultrafine powder obtained in the above step and lactose in a 1: 2 weight ratio.

The cordyceps ultrafine powder is further characterized in that it comprises the step of mixing the granulated Avicel PH 102 with a weight ratio of 1: 1.

Health supplements prepared through the manufacturing method may be prepared in the form of granular tea, tablets or pills.

Powder (granule) tea produced in the form shown in Figure 10 of the present invention is characterized by superior pharmacological effects including antioxidant, anti-inflammatory or anti-cancer activity compared to conventional Cordyceps tea bags.

In the present invention, the grinding mill that can solve the disadvantages of the generation of heat of crushing by friction of the conventional crusher, the grinding efficiency, such as fiber is not easy to crush and the loss of nutrients or active ingredients, loss of inherent color, flavor, etc. By using the high-speed rotary air flow type mill of Korean Patent No. 0515521 to prevent the destruction of the active ingredients such as dietary fiber, high fat due to the grinding of Cordyceps sinensis to produce a processed product in which the active ingredient is maximized color, aroma and flavor.

Hereinafter, the present invention will be described in more detail with reference to Examples, but the scope of the present invention is not limited to these Examples.

EXAMPLE

Example  1: Drying process for drying efficiency of Cordyceps sinensis

In order to improve the drying efficiency of Cordyceps sinensis, the drying efficiency of Cordyceps sinensis through vacuum drying and infrared drying, including conventional freeze drying, was investigated.

Cordyceps sinensis (Cordyceps) will be collected in Kangwon National Bank Cordyceps (EFCC) artificial fruit body composition ratio is 83% brown rice, pupa 17%, a water content of 69.62% wb.

Drying conditions of the Cordyceps sinensis and drying test conditions for each drying temperature are shown in Table 1.

The drying temperature of the freeze dryer is -45 ~ 55 ℃ and the vaccum pump speed is 648 L / min. Drying temperature range of the vacuum dryer: can be controlled up to 300 ℃, dried after reaching 760mmHg vacuum. Far-infrared dryer can control the temperature up to 200 ℃, the wind speed was dried at 1㎧. The moisture content was dried until it became constant at the temperature of 105 degreeC, and the moisture content was based on the moisture content.

Drying Method of Cordyceps Sinensis and Drying Test Conditions by Drying Temperature Drying method material; Cordyceps sinensis Drying Temperature (° C) and Conditions Freeze drying Raw materials Vacuum: 3 Torr, Cooling temperature: -50 ° C, Hot plate temperature: 35 ° C Vacuum drying Raw materials 60, 80, 100, 125, 150 Far Infrared Drying Raw materials 40, 60, 80, 100, 125, 150 badge 125 (dry up to 20% water content) → 80 (dry up to 10% water content) Cordyceps Cordyceps 125 (dry up to 20% water content) → 80 (dry up to 10% water content)

1. Lyophilization

According to the conditions of Table 1, 72 hours was required to reach a moisture content of about 5% of the test material with a moisture content of about 70% (freeze-dried sample was dried for 3 hours at 105 ± 5 ° C and again wetted under moisture content). Was taken. It was analyzed that the drying time is too long when the lyophilizer is dried using a lyophilizer, and that the drying cost is 1,300 won per 1k by using an expensive dryer.

2. Vacuum drying

In the case of a vacuum dryer of 760 mmHg in vacuum, the time required to reach a moisture content of 10% was measured at 21.5 hours and 12.7 hours, respectively. It was measured as 9.5 hours at 100 ° C. and 8.3 hours at 125 ° C. The drying time at 150 ° C. was measured as 5.2 hours. When drying the red sackcloth Cordyceps sinensis using a vacuum dryer, the optimum drying temperature range was 80 ~ 125 ℃.

However, when the vacuum dryer is used, the drying time is shortened, but there is a disadvantage that odor of the Cordyceps sinensis which is a problem even in lyophilization remains.

3. Far Infrared Drying

Cordyceps sinensis was found to be> 70%. As shown in FIG. 1, in the case of the drying temperature of 40 ° C., the water content was 18.3 hours to dry to about 10%.

The drying time for drying up to 10% moisture content was reduced to 16.2 hours at 60 ° C, 13.4 hours at 80 ° C, and 8.2 hours at 100 ° C. In the case of 125 ° C, it was shortened to 6.4 hours. Since the surface of the red sackcloth of the red stalk Cordyceps is burned by heat, it was found that the red sackcloth should be dried at <150 ° C.

Far-infrared dryer is a method of drying using radiant energy, the surface of the dried product after drying, the odor is removed, the color of cordyceps is preserved similar to the lyophilized sample, and the drying time was very short.

Therefore, after that, the samples of Cordyceps sinensis were separated into Cordyceps fruiting body part and medium part and dried according to the far-infrared drying method and used in the experiment. In the first drying, the drying time was maintained until the water content reached 20% at 125 ° C. After the water content reached about 20%, the second drying temperature was controlled to 80 ° C and dried until the dry weight was measured. .

The fruiting body part took about 20 minutes to dry at 125 ° C and had a water content of 74.4% to 17.5%. The water content took 20 minutes to dry up to 6% with continuous control at 80 ° C. That is, it can be seen that it can be dried in only 40 minutes for drying the bag portion. The media section took 1.5 hours to cut the size of the specimen into 1cm × 1cm size and dried from 71.8% to 25% of water content at 125 ℃ for the first time and 80 ℃ for the second drying in succession. It took 2 hours to reach 3.33% moisture content. That is, it was possible to dry up to 4% or less in 3.5 hours to dry the medium of Cordyceps sinensis.

In case of drying by far-infrared drying method, Cordyceps sinensis is dried up to 20% water content at 125 ° C of primary drying temperature and 5% or less at 80 ° C of secondary drying temperature in order to produce products with excellent economy, thermal efficiency and quality. The method could be analyzed as suitable.

Experimental Example  1: Antioxidant Effect of Cordyceps Sinensis by Drying Method

The purpose of this study was to investigate the antioxidant effects of dried Cordyceps sinensis according to the drying method and drying temperature, and to investigate the drying method and drying conditions with excellent antioxidant effect. Mix 100, 200, 400, 600, 800, and 1000 µg / ml of each sample of dried Cordyceps sinensis well with pH 7.4 PBS buffer to a total volume of 1000 µl, and 0.1 mM DPPH (1,1-Diphenyl-2-picryl hydrazyl) solution. 3 ml (99.5% ethanol dissolved) were added. After incubation at 37 ° C. for 30 minutes, the absorbance was measured on a spectrophotometer at 515 nm.

As shown in FIG. 2 and Table 2, even when the amount of samples of Cordyceps sinensis increased up to 1,000 mg / ml, the reduction power was very high as 95%, so that the sulfated efficacy was analyzed very little.

In the case of Cordyceps sinensis using vacuum dryer, when the amount of sample increased to 1,000mg / ml in the drying temperature range of 40 ~ 60 ℃, the reducing power was investigated to 65 ~ 67%, which improved the sulfateization efficiency, but in the sample whose drying temperature increased to 100 ℃ Reducing power was increased to 98% was able to analyze that the sulfate effect is very small.

In case of increasing the amount of dried Cordyceps herb samples to 1,000mg / ml using the far-infrared ray, the reducing power increased to 69% and 70 ~ 75% from 60 ~ 100 ℃ to the drying temperature. In the sample of, the reducing power was reduced to 54%, and the sulfateization efficiency was excellent. That is, it could be analyzed that the antioxidant activity of Cordyceps sinensis was very good at the drying temperature of 150 ± 25 ℃ of far-infrared dryer.

Determination of Antioxidant (DPPH) Effect by Drying Temperature of Cordyceps Sinensis Method (Freeze Drying, Vacuum Drying, Far-Infrared Drying) Drying Method Sample (mg / ml) Antioxidant (DPPH) Efficacy (%) Freeze drying Vacuum drying temperature (℃) Far Infrared Drying Temperature (℃) 40 60 80 100 40 60 80 100 125 150 175 0 100 200 400 600 800 1000 100 99.42 93.24 96.09 97.23 96.25 95.68 100 89.46 88.42 82.14 77.8 73.45 65.4 100 98.24 91.28 83.23 76.95 67.81 67.06 100 102 95.18 89.53 88.02 81.04 76.14 100 98.08 93.08 92 88.26 81.97 79.93 100 97.42 92.17 84.53 77.87 76.28 69.05 100 91.89 87.99 87.12 80.29 76.07 71.22 100 97.35 88.52 88.04 84.59 78.97 70.22 100 95.86 94.39 91.15 86.68 79.94 75.0 100 89.02 84.26 66.91 61.42 56.33 53.67 100 96.29 87.22 70.99 59.24 58.12 55.87 100 93.18 85.77 74.74 61.50 58.25 54.63

Example  2: of dried Cordyceps sinensis Ultra fine powder

In Example 1, it was intended to effectively crush and classify the Cordyceps sinensis dried by the far-infrared drying method as a powder tea.

The test material having a moisture content of 69.62wb.% Of the raw material was dried in a far infrared dryer until the water content was <10 wb.%. The dried test material was passed through a coarse crusher powder and passed through a 4 mesh sieve, and the passed sample was used as a test material for ultra fine powdering.

The ultra fine grinding machine used HKP-05 (Korea Energy Technology, Korea).

Grinding experiments of dried Cordyceps sinensis were carried out at a feed rate of 10 kg / hr, 80 and 90 kPa.

Experimental Example  One: Particle size analysis

The particle size distribution of the pulverized product was analyzed using a Particle Size Analyzer (Malvern Ins. Ltd, Mastersiser-2000, UK). The dry particle size distribution was measured three times using the dried test material.

 Particle analysis of the test materials also measured the values of cumulative particle size distribution, specific surface area, particle size distribution (Span), and average particle size based on volume, and the value of Span was measured by measuring the value of Span. It can be seen that the distribution of is wide. If the numerical value is small, the distribution range of the particle size can be analyzed. The Span equation is as follows (1998, Malvern Instruments Ltd).

D [V, 0.9]: Size when the volume of the tested sample is 90%

D [V, 0.1]: Size when the volume of the tested sample is 10%

D [V, 0.5]: median

The coarse powder passed through 2000㎛ (10 mesh), the fine powder was 420㎛ (40 mesh), the fine powder was 74㎛ (200 mesh), and the ultrafine powder passed 1 ~ 50㎛ according to the particle size of the pulverized product. It was analyzed by classifying.

As shown in FIG. 3, the particle size of the most frequent particles at the pulverization speed of 80 m / s was analyzed as a pulverized product having a specific surface area of 0.916 with a particle size distribution curve of 10 μm, and the average particle size was about 17 μm. When the grinding speed was increased to 90 m / s, the mono-modal particle size distribution curve with the most frequent particle size of 10 μm was shown. The specific surface area was 0.866 and the average particle size was 15 μm.

The particle size distribution of Cordyceps sinensis products in the cumulative particle size distribution of the crushed products according to the grinding speed is very fine that passes 95% of the <44 ㎛ (325 mesh) particle size, which is the ultra-fine grinding zone, in the grinding speed of 80㎧-90㎧. It could be analyzed that the powder could be produced.

The particle size average (d50) of the pulverized product was about 200㎛ at the grinding speed of 70㎧, and the technology development for mass production of coarse powder, medium fine powder, fine powder, ultra fine powder, etc. was developed as the grinding speed range of the ultra fine powder grinding machine increased. I could be sure.

Experimental Example  2: Observation of Characteristics of Grinding Products

In order to observe the characteristics of the pulverized product experimented with the ultra fine pulverizer, the fine structure of the raw material and the pulverized sample after drying were observed using the Energy Filtering-Transmission Electron Micrscope (LEO-912AB OMEGA).

As shown in Figure 4 (a), when the Cordyceps sinensis raw material is observed by TEM, the tissue is contracted and tightly densified. When the raw material is ultra finely ground using an ultra fine powder grinding machine, the cell wall tissue of the dried Cordyceps sinensis can be completely broken into pieces (Fig. 4 (b)).

As shown in Table 3, when the moisture content of the fruiting body and the medium of Cordyceps sinensis was examined, it was found that the content of water in the fruiting body was higher than the medium. This was found to be very good as a moisture source, and because there is a lot of moisture at the time of the experiment, there is a disadvantage that can cause a quantitative change of the extract through the change of the solvent conditions during ethanol extraction.

order sample Culture Plate (Before) Culture Plate + Dried Food Dry matter Water content Cordyceps sinensis Fruiting body? One 1.5682 0.2656 0.5959 0.3303 78.94 2 1.6024 0.2632 0.6238 0.3606 77.50 3 1.6308 0.2612 0.5973 0.3361 79.39 Average 78.61 Badge (below) 4 2.0355 0.2644 1.0184 0.754 62.96 5 3.4815 0.2661 1.4314 1.1653 66.53 6 2.1904 0.2743 1.0300 0.7557 65.50 Average 65.00 Overall weight 7 2.3128 0.2624 0.9714 0.709 69.34 8 2.0266 0.2658 0.8963 0.6305 68.89 9 2.5649 0.2697 0.9912 0.7215 71.87 Average 70.03

Experimental Example  3: solubility measurement

Cordyceps sinus was sent to Kangwon National University Biomaterials Laboratory, and it was stirred for 30 minutes depending on the temperature with the crushed sample by coarse crushing, ultra fine powder and extrusion molding, and centrifuged at 2,500rpm for 90 minutes. Centrifugation at 2,500 rpm for 60 minutes. The supernatant obtained therefrom was frozen in a deep freezer for one day, and then lyophilized to remove water and then weighed to obtain solubility. Solubility is expressed as a percentage of the amount soluble per 1g.

As shown in FIG. 5, the solubility was examined after the coarse and ultrafine milling of Cordyceps sinensis.

Experimental Example  4: Determination of Antioxidant Effects of Cordyceps Sinensis Extracts According to Grinding Method DPPH method )

Antioxidant effect was measured using Cordyceps sinensis powders, which were prepared by packing a powdered tea powder in a conventional tea bag, and Cordyceps sinus pulverized within an average particle size of 15 μm using an ultrafine mill.

Mix 50, 100, 250, 500, 1000, 1500, 2000, and 4000 µg / ml of each Cordyceps sinensis well with pH 7.4 buffer to a total volume of 1000 µL, and add 3 ml of 0.1 mM DPPH solution (99.5% ethanol dissolved). Add. After incubation for 30 minutes at 37 ℃ was measured on a spectrophotometer (515nm).

As shown in FIG. 6, the results of experiments comparing the antioxidant effects of Cordyceps sinensis extract with ethanol extract showed no significant difference by the effect of crushing in the ethanol extract, but in the case of water extract, the antioxidant effect was crushed by the ultrafine grinding method. It was found that the antioxidant effect was better than general crushing at.

Experimental Example  5: investigation of anti-inflammatory effects of Cordyceps sinensis

In general, macrophages are activated by LPS and various immune-inducing substances under external infection conditions. In this case, primary immune action occurs through prostaglandins, NO and cytokines secreted from macrophages. However, the primary immune action not only affects the external antigen but also damages the living body itself. To compensate for this disadvantage, the living body has a secondary immune response specific to the antigen. However, often the primary immune function is sustained, which is called inflammation. In this experiment, to determine the anti-inflammatory effect of Cordyceps sinensis, NO, an inflammatory factor that is reduced when LPS, Cordyceps sinensis water, and ethanol extract were treated together was measured. In addition, experiments were conducted with red ginseng, which is generally known as a herbal medicine.

NO production was indirectly confirmed by nitrite measurement. After 16 hours of administration of the sample to the cells, the medium was walked (100 μl), and Griess reagent (A; 1% sulfanilamide) of A and B was dissolved in naphtylenediamine in 0.1% 5% phosphoric acid. After reacting with a reagent mixed with 5% phosphoric acid (50 μl + 50 μl = 100 μl), the solution was measured at 550 nm, using sodium nitrite as a night light reference material. The secretion of nitrite of 264.7 cells was measured.

Macrophage RAW 264.7 cells were purchased from ATCC (ATCC T1B-71T ^MS ) and cultured in DMEM medium [penicillin (100 units / ml) and streptomycin (100 μg / ml)], and the culture conditions were maintained at 5% CO ₂ , 37 ° C. The incubator was incubated. After that, 2 × 10 ⁶ cells were planted in a 60 mm culture dish and tested after incubation for 24 hours.

Each fraction and single material of Cordyceps sinensis was dissolved in DMSO at 100 mg / ml and diluted with medium to each final concentration. And before each sample was added to the cells, LPS (1 μg / ml) and IFN-γ (50 U / ml) were added to the cells, and then each sample was administered to the cells.

As shown in Fig. 7, ethanol (7a) and water extract (7b) of Cordyceps sinensis showed the effect of inhibiting the production of NO and iNOS protein with better fine grinding than tea bags. Therefore, it can be seen that ultrafine grinding method maximizes the anti-inflammatory effect of Cordyceps sinensis more than the conventional tea bags.

Experimental Example  6: Fatty Acid Analysis of Cordyceps sinensis

After extracting Cordyceps sinensis with two solvents, ether and hexane, fatty acids were analyzed using GC (Hewlett packerd 6890 GC). The column used HP-INNOWAX. The temperature of the injector was 250 ° C. The temperature of the detector was 280 ° C. The column conditions were constant flow, and the temperature of the oven was raised to 14 ° C./min from 120 ° C. to 210 ° C. at first, and the temperature was increased to 7 ° C./min from 210 ° C. to 250 ° C.

As shown in FIG. 8, as a result of analyzing the fatty acid through GC, it was found that a large amount of unsaturated fatty acids were eluted in the ultra-milling method. In particular, it was found that linoleic acid and linolenic acid, which are not produced in vivo, are present in much larger amounts than other grinding methods, and thus may be used as an important fatty acid source.

Experimental Example  7: anticancer effect

To investigate the anticancer effect of Cordyceps sinensis using cancer cell line in The killing effect of cancer cells in vitro was tested by MTT method.

Cancer cell lines B ₁₆ F ₁₀ (melanoma) ATCC; CRL-6475 ^™ melanoma, HeLa (carcinoma) ATCC CCL-2; Cervical cancer, SKBR ₃ (Breast cancer) ATCC HTB-30; Breast cancer and A549 (lung carcinoma) KCLB 10185; Lung cancer cell lines were used. Normal cell lines include NIH3T3 (fibroblast) ATCC CRL-1658; Fibroblasts were used. All cell lines were cultured in 10% FBS DMEM or RPMI1640 medium (penicillin (100 units / ml), streptomycin (100 μg / ml)), and the culture conditions were incubated at 5% CO ₂ , 37 ° C. incubator.

Cells incubated in 10% RPMI / DMEM medium were trypsinized and seeded to 1 × 10 ⁴ cells / well in 96-well plates and grown in 10% FBS medium for 24 hours. After starvation in serum-free medium for 24 hours, the pupa Cordyceps sinensis extract was added, and after 48 hours, the extent of cell proliferation inhibition with 5 mg / ml MTT reagent was confirmed by absorbance at 570 nm in ELISA. Cordyceps sinensis samples used in the analysis were used by diluting 100 mg / ml extract using serum-free medium.

As shown in Table 4, the ethanol extract of Cordyceps sinensis was superior to the water extract.

Cancer cell line Cordyceps sinensis r ² = IC ₅₀ (μg / ml) B ₁₆ F ₁₀ cell 48hrs EE 0.91 264.9 EW 0.95 923.5 SKBR ₃ 72hrs EE 0.90 509.3923 EW 0.91 997.0576 HeLa cell 48hrs EE 0.98 478.7 EW 0.97 1722.3 A549 cell 48hrs EE 0.90 368.4 EW 0.92 1650.7

EE: ethanol extract; EW: water extract

Example  3: cordyceps Ultra fine powder  Product manufacturing

In general, Cordyceps sinensis is a situation in which raw materials are pulverized with a pin mill to make a coarsely pulverized powder and then packaged in a tea-bag or commercialized using a pill manufacturing method. In this embodiment, the ultrafine powders obtained in Examples 1 and 2 were granulated or tableted to enhance the product of Cordyceps sinensis, and the prototype manufacturing technology was studied in order to grasp the possibility of manufacturing technology that could mass-produce it.

The granulated product was mixed at a weight ratio of test material: lactose (200 mesh) = 1: 2, and then mixed with a solution containing 25% solubilized HPC (Hydroxy-propylcellulose) in water. Granule products were prepared using a granulator.

Cordyceps sinensis tablet preparation was prepared by mixing 1: 1 mixture of Avicel PH 102 granulated (microcrystallized) with test materials and tablet binder, and then mixing with v-mixer for 30 minutes by adding 1% Talc and then purifying with tablet press.

9 and 10 illustrate ultrafine powder prototypes, granule prototypes and tablet prototypes.

In conclusion, the present invention was to increase the water solubility through the ultra-fine powder method to enable convenient drinking, and to provide a cordyceps herb products that maximize the increase of unsaturated fatty acids, physiological activity (antioxidant, anti-inflammatory, anticancer effect).

As described through the above Examples and Experimental Examples, the present invention relates to a health supplement food using the ultrafine powder of Cordyceps sinensis and a method of manufacturing the same, the water solubility is increased by ultra-pulverizing Cordyceps sinensis dried through a far-infrared dryer, unsaturated fatty acid There is an excellent effect of providing a product that maximizes the pharmacological activity than the conventional tea bag product by increasing the production of functional products with increased antioxidant, anti-inflammatory and anti-cancer effects.

Claims (3)

In the production of food using cordyceps ultrafine powder, Firstly drying the Cordyceps sinensis in a far-infrared dryer at 125 ° C. to a water content of 20%, and drying the Cordyceps sinensis dried at 80 ° C. to a water content of 5% or less, Grinding the dried Cordyceps sinensis into a fine powder having a particle size of 44 μm or less by crushing at a raw material supply amount of 10 kg / hr and a grinding speed of 80 to 90 m / s, and Cordyceps sinensis ultrafine powder with enhanced pharmacological activity including antioxidant, anti-inflammatory or anticancer activity, comprising the step of preparing a granulated product by mixing the cordyceps ultrafine powder obtained in the step and lactose in a weight ratio of 1: 2 Method of manufacturing a health supplement using. The method of claim 1, wherein the Cordyceps Sinensis ultrafine powder further comprises the step of mixing with granulated Avicel PH 102 in a weight ratio of 1: 1, the pharmacological activity including antioxidant, anti-inflammatory or anti-cancer activity is enhanced Method of manufacturing health supplement foods using ultrafine Cordyceps sinensis. A health supplement using a Cordyceps sinensis ultrafine powder having an enhanced pharmacological activity including antioxidant, anti-inflammatory or anticancer activity in the form of granular tea, tablets or pills prepared according to the method of claim 1.

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