KR20080039420A - Use of 25-hydroxy vitamin d3 to improve male animal fertility - Google Patents

Abstract

The present invention relates to the use of 25-hydroxy vitamin D 3 to improve animal fertility. More particularly, the invention relates to the use of 25-hydroxy vitamin D3 for improving the quality of semen and/or conception rate in animals including men. In a preferred embodiment the invention relates to the use of 25-hydroxy vitamin D3 for improving the quality of semen in male and the conception rate in female animals as for example cattle, dogs, cats and horses, preferably pigs.

Description

USE OF 25-HYDROXY VITAMIN D3 TO IMPROVE MALE ANIMAL FERTILITY

The present invention relates to the use of 25-hydroxy vitamin D ₃ in improving fertility of animals.

In the framework of the present invention, animals means animals including mammals, examples of which include humans. Preferred examples of mammals other than humans are ruminants and non-ruminants such as pets such as cattle, dogs and cats, horses, camels, sheep, goats and pigs.

More specifically, the present invention relates to the use of 25-hydroxy vitamin D ₃ in improving the quality of semen in male animals, including men. In a preferred embodiment, the invention provides 25-hydroxy vitamin D ₃ in improving the quality and / or conception rate of semen in animals such as, for example, cattle, dogs, cats and horses, preferably pigs. It relates to the use of.

Very expensive genetically superior male animals, such as boars, stallions or bulls, are not only provided with special treatment and care, but must be kept as long as possible in order to maximize their function in these herds or in artificial insemination centers. Some ranches or artificial insemination centers now house these male breeding animals under optimized conditions to maintain their high performance, for example in bovine tunnel-ventilated or air-conditioned pig cages. Artificial insemination is carried out to maximize the function of male animals and ultimately to inherit their good heredity to many offspring. However, as generations of male animals progress, semen volume, sperm motility and sperm count tend to decrease gradually. Accordingly, fertilization rate and conception rate in female animals also decrease gradually.

According to the present invention, administration of 25-hydroxy vitamin D ₃ to boar improves the quality of boar semen and further increases conception rate in pigs fertilized by boars treated with 25-hydroxy vitamin D _3. It turned out.

Thus, in one aspect, the present invention relates to the use of 25-hydroxy vitamin D ₃ in improving the quality of semen, for example boar semen, and the conception rate of animals, for example pigs. In another aspect, the invention relates to a method of improving semen quality and conception in an animal comprising administering an effective amount of 25-hydroxy vitamin D ₃ to a male animal.

As used herein, the term "food product" refers to any food or feed suitable for human or animal consumption. The food product may be a manufactured and packaged human food (eg mayonnaise, salad dressing, bread or cheese) or an animal feed (eg extruded and pelletized animal feed, a blended or pet food composition). . The term "food" also includes solid and liquid foods, as well as drinking fluids such as beverages. As used herein, the term "foodstuff" refers to any substance suitable for human or animal consumption. The term "dietary supplement" refers to a small amount of a compound for supplementation of a human or animal diet packaged in single or multiple dose units. Dietary supplements generally do not provide significant amounts of calories, but may contain other micronutrients (such as vitamins or minerals). The term "nutrition supplement" refers to a composition comprising a dietary supplement with a source of calories. In some embodiments, the nutritional supplement is a meal replacement or supplement (eg, a nutrition bar, or an energy bar, or a nutritional drink or concentrate).

For the purposes of the present invention, 25-hydroxy vitamin D ₃ is suitably administered to the food as a supplement. Animal feeds may be mixed with regular food 25-hydroxy vitamin D ₃ , such as a commercial formulation available under the tradename ROVIMIX Hy-D®, or as a food ingredient and 25-hydroxy. Premixes of Roxy Vitamin D ₃ may be first prepared and then supplemented by mixing the premix with other food ingredients. For pigs, the food can be any conventional pig feed. In particular, 25-hydroxy vitamin D ₃ is a formulated powder that can be added to a premix containing other minerals, vitamins, amino acids and trace elements, which is added to a regular animal feed and thoroughly mixed and evenly distributed. To achieve.

In preparing the pig feed according to the present invention, about 10 mcg / kg to about 100 mcg / kg of 25-hydroxy vitamin D ₃ is added to the regular pig feed. Alternatively, food premixes are prepared based on the regular food ingredients by adding the active ingredient to the regular food ingredient at a higher concentration, for example from about 10 mg / kg to about 100 mg / kg of 25-hydroxy vitamin D ₃ . Can be. If 1 kg of this premix is added per 1000 kg of regular food, this will typically meet the individual needs of the animal by normal food consumption. For boars, preferred dosages range from about 50 to about 80 mcg of 25-hydroxy vitamin D ₃ per kg of food.

For the purposes of the present invention, 25-hydroxy vitamin D ₃ is suitably administered in an amount of about 0.112 mcg to about 1.120 mcg, in particular about 0.560 mcg to about 0.784 mcg per kilogram of body weight per individual animal. Thus, 25-hydroxy vitamin D ₃ is properly added to the food in an amount that meets these dosage requirements. Typically, boar feed may contain from about 10 mcg to about 100 mcg, in particular from about 50 mcg to about 80 mcg of 25-hydroxy vitamin D ₃ per kg of feed.

The effect of administration of 25-hydroxyvitamin D ₃ to boar on the quality of semen was determined from boar after administration of 25-hydroxyvitamin D ₃ , as well as total semen volume and consistency, bovine motility and sperm count of boar. The conception rate of pigs fertilized by semen can be determined by comparison with data obtained with feeds that do not contain 25-hydroxy vitamin D ₃ .

Typically in dogs 25-hydroxy vitamin D ₃ is suitably administered in an amount of from about 0.125 mcg to about 1 mcg per kilogram of body weight per individual animal, typically 25-hydroxy vitamin D ₃ in horses It is suitably administered in an amount of about 0.112 mcg to about 1.120 mcg per day of body weight per kg.

For consumption by humans, the food supplement according to the invention can be administered in unit dosage form, for example in tablets, capsules, metered powders or metered liquid volumes, which can be carbonated if desired. . Moreover, the food supplement of the present invention may further contain conventional inert and physiologically acceptable carriers, flavors, colorants and calcium. For example, purification may be supplemented by the amount of 25-hydroxy vitamin D ₃ that satisfy the daily dosage amount of about 5 to 15mcg 25-hydroxy-vitamin D ₃ of body weight per 1kg, where a typical daily The dose is one or two tablets.

The invention is further illustrated by the following examples.

Example 1 A typical pig feed comprising the ingredients set forth in Table 1 is supplemented with 50-80 mcg of 25-hydroxy vitamin D ₃ per kg of feed.

The food as specified above was 50-80 mg of 25-hydroxy vitamin D ₃ [4-6.4 g of Rovimix® Hi-D® 1.25%: DSM, Kayselaugst, Switzerland Nutrients Products] is supplemented with 25-hydroxy vitamin D ₃ by mixing with the rest of the food ingredients. The thick feed obtained can be pelletized if necessary.

Example 2 : Boar feed is prepared as indicated in Example 1 except adding about 50-80 mg of 25-hydroxy vitamin D ₃ per kg of vitamin and mineral premix.

Example 3 : A boar feed premix containing 25-hydroxy vitamin D ₃ can be prepared as follows.

All ingredients were carefully mixed together and this premix was added to the final feed at 0.5% (5 kg per 1000 kg food).

Alternatively, 25-hydroxy vitamin D ₃ may be added to a 1% diluted premix containing a suitable carrier. Such carriers may be wheat flour, coarse wheat flour, corn flakes, rice bran, almond shells or calcium carbonate alone, or various mixtures of several such carriers. Typical formulations are as follows:

Ingredient [%] Rice bran 64.20 Calcium carbonate 35.00 Rovimix® Hi-D® 1.25% 0.80

All ingredients were carefully mixed together and this premix was added to the final feed at 0.05% (0.5 kg per 1000 kg food).

Example 4 A conventional dog food (base diet) comprising the ingredients set forth in Tables 2 and 3 below was prepared at 10-80 mcg of 25-hydroxy vitamin D ₃ [Robimix® Hi-D ( 1.25%].

Nutritional composition of dog's base diet (Mera Dog "Brocken", Mera-Tiernahrung, Germany De-47625 Kebera-Bethen Marienstrasse 80-84) ingredient Inclusion level protein, % 24 Fat, % 9.0 fiber, % 3.0 Ash,% 7.0 calcium, % 1.3 sign, % 1.0 salt, % 0.4

Vitamin composition of base diets (Mera Dog "Broken", Mera-Tiernachrunge Gembeha, Germany De-47625 Kebera-Benten Marienstrasse 80-84) Vitamin / kg Inclusion level Vitamin A, IU 12000 Vitamin D ₃ , IU 1200 Vitamin E, mg 95 Vitamin B ₁ , mg 3.2 Vitamin B ₂ , mg 5.6 Vitamin B ₆ , mg 3.2 Vitamin B ₁₂ , mcg 64 Pantothenic acid, mg 22 Niacin, mg 20 Folic acid, mg 0.5 Biotin, mcg 200 Choline chloride, mg 1600

Example 5 A typical horse feed (base diet) comprising the ingredients set forth in Tables 4 and 5 below was prepared from about 0.112 mcg to about 1.120 mcg of 25-hydroxy vitamin D ₃ [Robimix® per kg of body weight. Hi-D® 1.25%] in a quantity that satisfies the daily dosage of 25-hydroxy vitamin D ₃ .

Nutritional composition of the base diet of horses ingredient Inclusion level protein, % 13.5 Fat, % 3.6 fiber, % 9.7 Ash,% 5.9 calcium, % 1.3 sign, % 0.5 salt, % 0.3

Vitamin composition of the base diet of horses Vitamin / kg Inclusion level Vitamin A, IU 36000 Vitamin D ₃ , IU 3000 Vitamin E, mg 1000 Vitamin B ₁ , mg 15 Biotin, mcg 500

Example 6 Dietary Supplement for Human Consumption in Tablet Form may include 25-hydroxy vitamin D ₃ in a matrix component standardized for the vitamin preparation. Standard matrix components may comprise natural polysaccharide rubbers having emulsifying performance, or mixtures of various natural polysaccharide rubbers having emulsifying performance without any mixture of proteins.

Ingredient Refined sugar content 25-hydroxy vitamin D ₃ (crystalline) 10-125mcg Matrix component (standardized) 100-400 mg Calcium salt 0-1000mg

Tablets may be prepared and formulated by conventional tableting means known to those skilled in the art. Thus, for example, tablets can be made by mixing, grinding and grinding all the ingredients of a food supplement and pressing the granules in a tablet press.

Claims (14)

Use of 25-hydroxy vitamin D ₃ in improving the quality and / or conception of semen in animals. The method of claim 1, Use in improving the quality of semen in pets, preferably dogs and cats. The method of claim 1, Use in improving the quality of stallion semen in horses. The method of claim 1, Use in improving the quality of bull semen in cattle. The method of claim 1, Use in improving the quality of boar semen and conception rate in sows. The method of claim 1, Use in improving the quality of semen in humans. The method according to any one of claims 1 to 6, Said 25-hydroxy vitamin D ₃ is administered as a food supplement. The method of claim 7, wherein The 25-hydroxy vitamin D ₃ is added to the food in an amount necessary to administer about 0.1mcg to about 1.2mcg, especially about 0.5mcg to about 0.8mcg of 25-hydroxy vitamin D ₃ per kilogram of body weight per individual animal. Use added. The method of claim 8, Said 25-hydroxy vitamin D ₃ is added to the boar feed in an amount of about 10 mcg to about 100 mcg, in particular about 50 mcg to about 80 mcg per kg of feed. A method of improving the quality of male semen, comprising administering to the male animal an effective amount of 25-hydroxy vitamin D ₃ . The method of claim 10, Administering said 25-hydroxy vitamin D ₃ in an amount of about 0.1 mcg to about 1.2 mcg, especially about 0.5 mcg to about 0.8 mcg per kg body weight per day. The method of claim 10, Administering said 25-hydroxy vitamin D ₃ as a food supplement. A method of improving the quality of boar semen and increasing fertility in sows, comprising administering to the boar an effective amount of 25-hydroxy vitamin D ₃ . The method of claim 13, Wherein said 25-hydroxy vitamin D ₃ is administered in an amount of from about 10 mcg to about 100 mcg, in particular from about 50 mcg to about 80 mcg per kilogram of body weight per individual animal.