KR20070089474A - Cosmetic composition for controlling anti-acne and anti-comedo - Google Patents

Abstract

A composition comprising an extract fermented by lactic acid bacteria isolated from Kimchi is provided to inhibit the growth of microorganism involving with the generation of skin acne and skin inflammation rapidly and improve the acne skin by inhibiting excessive sebum secretion and having anti-inflammatory effect. The cosmetic composition for preventing and improving acne comprises an extract fermented by lactic acid bacteria isolated from Kimchi such as Leuconostoc mesenteroides, Leuconostoc pseudomesenteroides, Leuconostoc lactis, Lactobacillus brevis, Lactobacillus plantarum and Leuconostoc kimchi. The composition further comprises at least one selected from the group consisting of an extract of soybean, a fermented extract of soybean, a culture concentration solution of fermented extract of soybean, and a dried material of fermented extract of soybean culture solution.

Description

Cosmetic composition for improving acne skin {Cosmetic composition for controlling anti-acne and anti-comedo}

The present invention relates to a cosmetic composition for improving acne skin having a kimchi lactic acid bacterium fermentation extract and a soybean fermentation extract, and the like, having an antibacterial effect against sebum causing bacteria, a sebum secretion inhibitory effect and an anti-inflammatory effect.

Acne is an inflammatory disease of the fur branch, hair follicles and surrounding tissues caused by hormone action, excessive sebum secretion, bacterial infections, and the like.

The causes of acne can be divided into several ways.

The first cause is the excessive secretion of sebum. Sebum is a substance secreted by the sebaceous glands, and in normal amounts it acts to prevent harmful substances outside the skin from penetrating keratinocytes. However, under puberty, before and after menstruation, early pregnancy, contraceptives, and stress, the human body shows a different hormone balance than normal. This altered hormonal balance stimulates the sebaceous glands, which leads to an oversecretion of sebum. In addition, sebum secretion may be genetically increased and may be increased by changes in external temperature or skin temperature caused by poor eating habits.

The second cause is infection of the pores. Excessively secreted sebum contains a lot of fatty acids to increase the pH of the skin surface, dissolving the lipids of the skin lowers the defense of the skin itself. On the surface of the skin with high pH, acne-causing bacteria become easy to multiply, and the multiplying acne bacteria cause purulent inflammation through weakened skin.

In general, acne can be divided into four stages according to symptoms. The first stage is scleroderma acne, in which excessive secretion of sebum accumulates in the pores around the sebaceous glands, forming a scalp. Stage 1 spontaneous acne is an early stage of acne lesions but cosmetically not good skin trouble. Good care at this stage can also inhibit the development of inflammation. Stage 2 Papular Acne The acne germs multiply and cause inflammatory reactions that cause red acne, which is called papules and can cause scars if not properly managed. In the third stage purulent acne, the inflammatory reaction of red acne becomes more severe, and pus inside is formed. This is called pustules. In this stage, scars may remain even after treatment, so professional treatment is required. The final stage 4 nodular acne is a stage in which the pus ruptures inside the skin, causing more inflammation around the acne affected area, which leads to a mass of fingers. This is called nodule. It is a time when the inflammation penetrates the dermis and requires specialized treatment. Can be prolonged.

Treatment of acne includes topical formulations and oral medications, and surgical treatments such as laser and dermabrasion that have recently been performed.

Topical application therapy uses benzoyl peroxide for antibacterial effects such as azelaic acid and salicylic acid for the purpose of antibacterial action against propionibacterium acnes, which is known as the cause of acne. peroxide) is used as external ointment type. However, azelaic acid is difficult to formulate and salicylic acid is classified as a preservative in Korea, there is a problem that can not contain more than 0.5% in the formulation. In the case of benzoyl peroxide, there is a problem that it cannot be used as a cosmetic raw material in Korea.

Oral drug therapy uses retinoids and corticosteroids to inhibit sebaceous gland function and inhibit hair follicle wall destruction. These preparations have side effects such as skin rash, dry skin, and allergies may occur depending on the individual.

The problem of already developed, advanced acne and other skin diseases can be said that the external preparations used directly on the skin are more effective considering various aspects such as the speed and economical efficiency of the skin (see Korean Patent Publication No. 2004-0056081).

There are not many substances that can see these medical effects at the cosmetic stage. However, it can be used for a long time and has less damage to normal skin than a therapeutic drug. Therefore, many plant extracts and synthetic materials have been patented as a composition for improving acne skin, but use <Korean Patent Publication Nos. 2005-0081564, 2005-0081550, 2005-0073027, 2005-0071275, etc.> In addition to the therapeutic effects such as skin exfoliation later, the side effects are also high, so there are not many products that are safe for the skin and show the desired effect.

The present inventors found that the culture solution of Leukonostock Kimchi I was excellent as an antimicrobial agent during the recent research and patent investigation (Domestic Patent Publication No. 2004-0011695). In the above invention, Kimchi lactic acid bacteria fermented extract (Lyukonstock kimchi eye culture medium) was a substance having a wide range of antimicrobial activity against various bacteria, but the antibacterial activity against propionibacterium acnes, which is the cause of acne, has not been verified and is essential for acne improvement. Efficacy in controlling sebum secretion has also not been demonstrated. In addition, the soybean extract of fermented extract by Bifidus Lactobacillus is known to improve the environment of the skin, but the effect of reducing sebum secretion and preventing the infection of pores has not been verified.

The present invention contains a Kimchi Lactobacillus fermentation extract, a natural antibacterial substance, has the effect of controlling the sebum secretion essential for acne improvement as well as the antibacterial effect against the acne causing bacteria, and further by containing soybean fermentation extract, It is an object of the present invention to provide a cosmetic composition for improving acne skin, which has an effect of improving acne in a wide range of stages by synergy with an extract.

In order to achieve the above object, acne skin improvement cosmetic composition according to the present invention is characterized in that it contains kimchi lactic acid bacteria fermentation extract.

In the cosmetic composition for improving acne skin according to the present invention, the kimchi lactic acid bacteria are Leuconostoc mesenteroides, Leuconostoc pseudomesenteroides, Leuconostoc lactis (Leuconostoc lactis), Lactobacillus It is characterized in that at least one selected from the group consisting of brevis (Lactobacillus brevis), Lactobacillus plantarum (Lactobacillus plantarum) and Leukonostoc kimchii (Leuconostoc kimchii).

In the cosmetic composition for improving acne skin according to the present invention, the composition further contains at least one selected from the group consisting of soybean extract, soybean fermentation extract, culture concentrate of soybean fermentation extract, and dried product of the culture solution of soybean fermentation extract. Characterized in that.

In the cosmetic composition for improving acne skin according to the present invention, the soybean fermentation extract is characterized in that the soybean extract is a bifidus lactobacillus fermentation broth.

The cosmetic composition for improving acne skin according to the present invention is characterized in that it is the use of antibacterial acne causing bacteria propionibacterium acne, the use of controlling sebum secretion, the use of reducing scleroderma, and anti-inflammatory.

Cosmetic composition for improving acne skin according to the present invention is characterized in that it has a formulation of softening longevity (skin lotion), nourishing longevity (milk lotion), nutrition cream, massage cream, pack, gel, lipstick, makeup base or foundation.

Hereinafter, the present invention will be described in more detail.

Kimchi, a traditional Korean fermented food, has a taxonomic group of lactobacillus from various groups, such as leukonostock mesenteroid, leukonostock pseudometheroidoid, leukonostock lactis and lactobacillus brevis, lactobacillus plantarum, etc. A group has been discovered, and a new lactic acid bacterium called Leukonostock Kimchia has been discovered. <International Journal of Systematic & Evolutionary Microbiology, 50, 1915 ~ 1919, 2000 >.

In the present invention, the kimchi lactic acid bacterium fermentation extract may further contain a soybean extract, soybean fermentation extract, soybean fermentation extract culture concentrate, or a dry matter of the soybean fermentation extract culture medium, so that the soybean extract bifidus lactic acid bacteria fermentation solution, that is, Soybean fermented extract was prepared through the following process.

Soybeans are obtained by washing the soybean extract using centrifugation and microfiltration with an aqueous solution of sodium chloride under conditions of no hydrolysis after washing with soybeans. After incubating Bifidobacteria in a vegetable medium at 35 ° C., cell mass is collected and washed with 0.9% aqueous sodium chloride solution. After washing, biomass is collected to maintain the concentration of 100 million B. pylori bacteria per 1 ml of soy extract. Soybean fermentation extract is obtained by separating Bifidobacteria by high pressure homogenization method and purifying the residual material by membrane filtration and high pressure homogenization.

Soybean extract obtained through the above manner, the bifidus lactic acid bacteria culture solution (soybean fermentation extract), soybean extract of the soybean extract, the concentrated solution of the culture solution or dried product of the culture solution was used with the Kimchi lactic acid bacteria fermentation extract for the purpose of improving acne skin in the present invention.

Acne that develops and develops for multiple reasons is not only improved by antibacterial activity against acne. That is, a significant improvement can be made only when the effects such as sebum secretion and alleviation of the inflammatory response are accompanied.

The present invention by using the kimchi lactic acid bacteria fermentation extract of the above, and further by using a fermented extract of soybeans and kimchi lactic acid bacteria fermentation extract, showing a rapid sterilization effect on propionibacterium acnes, which is the cause of acne, causes of acne This will control the sebum secretion, will alleviate inflammation.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail based on an Example. However, the present invention is not limited by the following examples.

<Example 1-3 and Comparative Example 1-4>

According to the components and the content (% by weight) shown in Table 1 below, Examples 1 to 3 and Comparative Examples 1 to 4 were prepared. Specifically, Example 1 is a combination of kimchi lactic acid bacteria fermentation extract, soybean extract, and soybean fermentation extract as an active ingredient (phase C in Table 1) of acne skin improvement, Example 2 is a kimchi lactic acid bacteria fermentation extract and Soybean fermented extract is blended, and Example 3 contains only kimchi lactic acid bacteria fermented extract. In addition, Comparative Example 1 does not contain any active ingredients for improving acne skin, Comparative Example 2 is a reference material to be used as a reference for the antimicrobial activity, containing clindamycin that is often used as an acne treatment, Comparative Example 3 contains only soybean extract, and Comparative Example 4 contains only soybean fermented extract.

In this embodiment, the Kimchi Lactobacillus fermentation extract is a natural antimicrobial agent that contains a culture solution, a concentrated solution of a culture medium, or a dried product of a culture solution of Leukonostock Kimchi Eye, which is listed in CTFA as a cosmetic raw material. A brand name Luciddal PT-35 was used. The raw material is a material of the Republic of Korea Patent Publication No. 10-2005-0083098 commercialized as a raw material for cosmetics.

The manufacturing method of Examples 1-3 and Comparative Examples 1-4 is as follows.

The components of phase A in Table 1 were completely dissolved, and the components of phase B were completely dissolved in a separate dissolution tank, followed by mixing solubilization by adding phase B to phase A. The ingredients of phase C were added thereto according to the mixing ratios described in Table 1, homogenized and filtered to prepare the compositions.

Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 A Deionized Water TO 100 TO 100 TO 100 TO 100 TO 100 TO 100 TO 100 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 B ethanol 5.0 5.0 5.0 5.0 5.0 5.0 5.0 PEG-60 hydrogenated castor oil 0.4 0.4 0.4 0.4 0.4 0.4 0.4 Perfume 0.04 0.04 0.04 0.04 0.04 0.04 0.04 C Lucidal PT-35 0.4 1.0 1.0 - - - - Soybean Extract - - - - - 1.0 - Soybean Fermented Extract 0.5 1.0 - - - - 1.0 Clindamycin - - - - 1.0 - -

Experimental Example 1 Antibacterial Activity Test against Acne Bacteria

Test the antimicrobial activity against propionibacterium Acnes <ATCC 6919: Medium-BHI broth>, acne-causing strain with each cosmetic composition prepared in the composition of Examples 1-3 and Comparative Examples 1-4 It was.

The antibacterial test method for acne bacteria was as follows.

(1) Preparation of test bacteria

Propionibacterium acnes was used as a culture broth inoculated in BHI broth and anaerobic culture.

(2) dilution solution preparation

0.15 ml of the test bacteria was added to 15 ml of BHI broth (pH 6.8) or LB broth (pH 4.5) and mixed well as a dilution solution.

(3) Sample preparation

The cosmetic composition undiluted | stock solution manufactured from Examples 1-3 and Comparative Examples 1-4 was used as a sample.

(4) antibacterial test

1) Insert the sample to the starting concentration in the first well of 96-well cell culture tube (96well plate) and adjust the total amount to 200ul with the diluted solution. Into the remaining wells add 100ul of diluted solution.

2) Mix the mixed solution of the first row well, take 100ul and add it to the second row, mix well, and then take 100ul again and add it to the third row to double dilution.

3) After standing incubation for 24 hours and 48 hours at 32 ℃, determine the growth of bacteria to the extent of suspension and determine the minimum concentration without growth of bacteria as MIC (Minimun Inhibitory concentration) value. If the mixed solution is opaque and it is difficult to determine the growth of bacteria, check through a microscope.

The antimicrobial activity test results for acne bacteria are shown in Table 2 below.

Item pH Propionibacterium Acnes Example 1 5.7 <20 ppm Example 2 5.5 <1 ppm Example 3 5.7 <5 ppm Comparative Example 1 5.7 * Cannot be measured Comparative Example 2 5.5 <10 ppm Comparative Example 3 5.7 <30 ppm Comparative Example 4 5.7 <30 ppm

Example 1, Example 2, and Example 3 had an antimicrobial activity of 20 ppm to 1 ppm of MIC against the test bacteria. The lower the concentration of ppm in MIC, the more effective the substance against acne. In addition, the results showed that the antibacterial activity was higher when the kimchi lactic acid bacteria fermentation extract, soybean extract and soybean extract were not used at all (Comparative Example 1) (Example 3, Comparative Example 3 and Comparative Example 4), In addition, when the kimchi lactic acid bacteria fermentation extract, soybean extract and soybean fermentation extract were used alone (Example 3, Comparative Example 3 and Comparative Example 4) when mixed with kimchi lactic acid bacteria fermentation extract and soybean fermentation extract (Example 2) or When mixed with kimchi lactic acid bacteria fermentation extract, soybean extract, and soybean fermentation extract (Example 1) it was confirmed that the antimicrobial activity of the standard material (Comparative Example 2) similarly increased.

<Experimental Example 2: Test for improvement of acne, reduction of sebum secretion and stimulation>

Twelve people who had acne were allowed to use the cosmetic compositions prepared from Examples 1-3 and Comparative Examples 1-4 for one month.

The acne improvement scale is from 1 to 5 points, 1 point is not, 3 points are normal, and 5 points are very true. The experimental results are expressed in the average score of 12 people in Table 3 below.

Acne disappearance was based on the number of days the extinction was read and acne recurrence was based on the results after one month.

Sebaceous glands are reduced from 1 to 5 points, 1 point is not, 3 points are normal, and 5 points are very true. Experimental results are shown in the average score of 12 people in Table 3 below.

The presence or absence of skin irritation was determined as (number of irritants) / (total number of test subjects).

Inflammatory Acne Improvement Cotton acne extinction Acne recurrence Reduce sebum secretion Irritation Example 1 4.2 5 days radish 4.0 1/12 Example 2 4.5 2 days radish 4.1 2/12 Example 3 3.0 10 days radish 3.5 3/12 Comparative Example 1 2.1 13th U 2.0 0/12 Comparative Example 2 4.2 2 days radish 4.12 9/12 Comparative Example 3 3.1 9th U 3.2 1/12 Comparative Example 4 3.5 10 days radish 3.3 2/12

As shown in Table 3, compared to Example 3, Comparative Example 1, Comparative Example 3 and Comparative Example 4, Example 1 and Example 2 did not recur acne, average 4 points or more for inflammatory acne improvement, scrim The average acne extinction time is 5 days or less, sebum secretion reduction average 4 points or more is obtained, it can be seen that there is an excellent effect on acne improvement. On the other hand, Comparative Example 2 containing an antimicrobial activity standard, showing similar efficacy as Examples 1 and 2, but there is a risk of skin irritation in the long-term use because the stimulation is stronger than the other experiments in use .

Therefore, in view of various factors such as irritation, rate of improvement, degree of improvement, and reduction of sebum secretion, when Kimchi Lactobacillus fermentation extract and soybean fermentation extract are mixed (Example 2), or Kimchi Lactobacillus fermentation extract and soybean extract, soybean fermentation It can be seen that the combination of extracts (Example 1) is suitable for patients with acne skin.

Experimental Example 3: Inflammation Improvement Effect

The anti-inflammatory effect was evaluated by the inhibitory effect of the production of prostaglandins.

The effect was measured on macrophages using the electro-extracted extract. First, aspirin was added to macrophages taken from the abdominal cavity of mice to a final concentration of 500 M to irreversibly inhibit the cyclooxygenase (COX) activity remaining in the cells. Then, 100 μl of the suspension was added to each well of a 96-well cell culture tube and incubated for 2 hours in an incubator at 37 ° C. with 5% CO ₂ to attach macrophages to the container surface. The attached macrophages were then washed three times with PBS and used for the effect test of the extract. Incubated for 12 hours by adding RPMI medium containing 1% (w / v) of LPS to 5 × 104 cells / ml of electrocultured macrophages, inducing prostaglandin production and processing 100 μl of the extract to enzymatically immobilize the free prostaglandins. Quantification was carried out using an assay (ELISA).

In this case, the production inhibitory activity of the prostaglandin of the extract was set to 100% the difference between the prostaglandin produced in the LPS-treated and untreated groups, respectively, and treated with LPS and the sample to reduce the percentage of prostaglandin The results were compared and determined, and the results (the effect of inhibiting the production of prostaglandins) are shown in Table 4 below.

Blank 100% Control group (aspirin treated group) 25.0% Example 1 33.6% Example 2 44.2% Example 3 40.2% Comparative Example 1 21.4% Comparative Example 2 24.2% Comparative Example 3 15.5% Comparative Example 4 18.4%

As shown in Table 4, as a result of the experiment, it can be seen that the inhibitory effect of the production of prostaglandins according to Examples 1, 2, and 3 was significantly higher than that of the control treated with aspirin, and also soybean extract (Comparative Example 3) It was found that the effect of inhibiting the production of prostaglandins by fermentation extract (Comparative Example 4) was high.

As mentioned above, the cosmetic composition for improving acne skin according to the present invention primarily exhibited its effect by rapidly inhibiting the growth and growth of microorganisms involved in skin acne development and skin inflammation, and secondly, acne. Acne skin has been improved by suppressing excess sebum secretion, which is one of the causes of development, and extinguishing vaginal acne. It also has an anti-inflammatory effect, improving acne skin.

Claims (9)

Cosmetic composition for improving acne skin, characterized by containing kimchi lactic acid bacteria fermentation extract. The method of claim 1, The kimchi lactic acid bacteria is characterized in that at least one selected from the group consisting of leuconosstock methylene terroid, leuconosstock pseudometheroid, leuconosstock lactis, Lactobacillus brevis, Lactobacillus plantarum and leuconosstock kimchi eye Cosmetic composition for improving acne skin. The method of claim 1, The composition is a cosmetic composition for improving acne skin, characterized in that it further contains at least one selected from the group consisting of soybean extract, soybean fermentation extract, soybean fermentation extract culture concentrate, and dried soybean fermentation extract culture medium. The method of claim 3, The soybean fermented extract is a cosmetic composition for improving acne skin, characterized in that the soybean extract of bifidus lactic acid bacteria fermentation. The method of claim 1 or 3, The composition is a cosmetic composition for improving acne skin, characterized in that the use of antimicrobial acne causes propionibacterium acnes. The method of claim 1 or 3, The composition is a cosmetic composition for improving acne skin, characterized in that the use for controlling sebum secretion. The method of claim 1 or 3, The composition is a cosmetic composition for improving acne skin, characterized in that the use for reducing the generation of cotton. The method of claim 1 or 3, The composition is a cosmetic composition for improving acne skin, characterized in that the use of anti-inflammatory. According to claim 1 or 3, wherein the composition is characterized in that it has a formulation of softening longevity (skin lotion), nourishing longevity (milk lotion), nutrition cream, massage cream, pack, gel, lipstick, makeup base or foundation Cosmetic composition for improving acne skin.