KR20070081211A - Composition of hair tonic and prevention of hair loss - Google Patents

Abstract

A composition for hair tonic and prevention of hair loss is provided to continuously maintain the hair tonic effects even after hair grows above a certain level, and inhibit repeated hair loss after stopping its application. The composition for hair tonic and prevention of hair loss comprises 1-10 wt.% of Glycyrrhiza uralensis Fisch., 20-40 wt.% of Angelica gigas Nakai, 10-20 wt.% of Acorus gramineus, 20-40 wt.% of Cnidium officinale, 10-20 wt.% of Polygonum multiflorum Thunberg, and 19-30 wt.% of Coix lachryma-jobi var. ma-yuen, and is applied to the scalp in the amount of 1-100mg per day.

Description

Composition of hair tonic and prevention of hair loss

1 is a photograph showing the results of male C57BL6 mouse experiment according to the present invention,

Figure 2 is a photograph showing the results of the female C57BL6 mouse experiment according to the present invention,

Figure 3a shows a photograph showing the morphological changes of the comparative male C57BL6 mouse hair follicles,

Figure 3b shows a picture showing the morphological changes of male C57BL6 mouse hair follicles according to the present invention,

Figure 3c shows a photograph showing the morphological changes of the positive control male C57BL6 mouse hair follicles,

Figure 4a shows a picture showing the morphological changes of the comparative female C57BL6 mouse hair follicles,

Figure 4b shows a picture showing the morphological changes of female C57BL6 mouse hair follicles according to the present invention,

Figure 4c shows a picture showing the morphological changes of the positive control female C57BL6 mouse hair follicles.

The present invention relates to wool and anti-hair loss agents. The present invention relates to wool and hair loss prevention agent, in particular, in addition to wool and hair loss prevention effect, wool continues even after a certain level of hair growth progresses, and hair loss does not occur even if the drug application is stopped after hair loss prevention treatment.

In male expression, testosterone is involved in male impulse, skeletal muscle increase, male external genital development, scrotum growth, and spermatogenesis, and dehydrotestosterone in acne, sebum increase, hair loss, and prostatic hyperplasia. It is known that research on hair growth, wool and hair loss prevention has begun for a long time, but the mechanism of hair growth and hair loss is not yet known. However, the hair is about 100,000 to 150,000 pieces, the hair is grown and dropped through the growth phase, the degenerative phase, the resting period, each having a different cycle. This cycle is repeated over three to six years, with an average of 50 to 200 hairs dropped out per day. In general, alopecia refers to a shortage of hair growth in the growth phase and more hair in the degenerative or resting phase during this cycle, leading to a higher number of abnormal hair dropouts.

Alopecia, in which hair falls from the scalp, is known to be caused by a number of factors, including internal constitution such as genetic constitution or the action of male hormones, mental stress in everyday life, and accumulation of lipid peroxide in the scalp. There are external factors, and these factors are known to be intricately related to the symptoms of hair loss. In recent years, hair loss in young people, including obesity hair loss of women as well as male hair loss has been gradually spread, and many kinds of hair growth or wool agents are commercially available to improve the hair loss phenomenon.

The main ingredients mainly used in commercial hair growth or wool agents include vasodilators such as capronium chloride, minoxidil, and extracts of various plants to circulate enough blood to the scalp, or the action of male hormones that adhere to the nipples and eliminate hair. There are female hormones, such as estrogen, estradiol, progesterone, or male hormone activation inhibitors, such as pentadecanoic acid and finasteride, for inhibiting. For example, two drugs that are approved by the US Food and Drug Administration (FDA) as a hair loss treatment agent are Minoxidil, which is a treatment applied to hair loss, and Pinasteride, which is used orally, has excellent anti-hair loss effect by inhibiting the activation of testosterone. It is used a lot in.

However, most of the hair growth and wool agents described above have some effects on hair loss prevention, but the effects related to hair growth have not been satisfactory. That is, these preparations are effective only in the area where the hair roots are activated, so the hair loss prevention effect is significant, but the hair growth, or hair growth effect, of hair that has been at rest for a long period of time is insignificant. It is known to be ineffective.

In addition, the above-described preparations not only do not progress hair growth if not administered after a certain level of hair growth, and also remains a problem that hair loss occurs if not continuously administered even after hair loss prevention.

The present invention effectively prevents hair loss, promotes hair growth, and maintains the effect thereof, thereby maintaining wool after a certain level of hair growth, and reversing hair loss and hair loss prevention agent even if the drug application is stopped after hair loss prevention treatment. To provide.

In order to achieve the above object, the present invention provides a composition for preventing wool and hair loss of herbal extracts, including licorice, Angelica, irises, Cheongung, Sewao and Euiin.

Hereinafter, the present invention will be described in detail.

Licorice in the herbal medicine according to the present invention may have the effect of anti-inflammatory, anti-male hormone, etc., Angelica has the effect of improving the blood circulation of the scalp and clear the blood, the bluepo is said to make the hair shiny and not fall out since ancient times. , Sewage can have the effect of tonic blood, nutrients, regeneration of the follicle, the right person is known to have analgesic, soothing, jingyeong, antipyretic and insecticidal action, and most of the herbal medicines to prevent atherosclerosis, thrombolysis by cholesterol inhibition It is used in part, such as lowering blood pressure and peripheral blood vessel expansion, and is expected to help nourish and supply oxygen to hair root cells.

In addition, one of the main components of the present invention, uiyiin is the main component of fat oil is composed of myristric acid (myristric acid), beta-sitosterol (β-sitosterol), campesterol (campesterol), stigmasterol (stigmasterol), etc. Among the components, myristric acid and beta-sitosterol have been reported to inhibit 5-alpha-reductase. Licorice is composed mainly of terpenoids such as glycyrrhetinic acid, glycyrrhetizin, and flavonoids. Among these compounds, glycyrrhetinic acid Glycyrrhetizin has also been reported to inhibit 5-alpha-reductase.

However, the herbal medicines according to the present invention are in addition to the hair and hair loss prevention effect, the hair continues after a certain level of hair growth, and the hair loss and hair loss prevention effects are not known even if the drug application is stopped after hair loss prevention.

The present invention contains 1-10% by weight licorice, Angelica 20-40% by weight, irises 10-20%, Cheongung 20-40%, Sewage 10-20% by weight and 19-30% by weight Preferably, the mixing ratio may be arbitrarily determined within the scope of the present invention.

The herbal extracts may be used after extraction by immersion in an extraction solvent or by boiling water and filtered. At this time, each herbal may be mixed and used after extraction, or may be used after a separate extraction process for each herbal.

In order to use the effective medicine, it is preferable to add purified water to the herbal medicine, extract with hot water, filter, concentrate and store at 4 ° C.

In addition, the preparation using the herbal composition according to the present invention as an active ingredient is preferably used as a liquid composition containing 3 to 20% by weight of the herbal composition as an active ingredient and directly applied to the scalp, and can be prepared and used as a shampoo. have.

The herbal composition as an active ingredient of the present invention is preferably used 1 to 100mg per day divided into 1 ~ several times to be applied to the scalp and absorbed well.

By the following examples will be described in detail the present invention. It is apparent in the art that this example is only for illustrating the present invention and the scope of the present invention is not limited to these examples according to the gist of the present invention.

< Example  1> Preparation of herbal composition according to the present invention

Buy commercially available licorice, Angelica, Cheongpo, Cheongung, Sewao and Euiin, wash them clean, mix 0.1kg, Angelica 0.66kg, Iris 0.3kg, Cheongung 0.66kg, Sewage 0.5kg and Euiin 0.3kg Hot water was extracted for 2 hours with 6 times the weight of water, followed by filtration to obtain a primary hot water extract. Water was added to the residue three times the weight of the crude medicine, and the second hot water extract and filtered for 2 hours to obtain a second hot water extract. The first hot water extract and the second hot water extract were mixed and concentrated under reduced pressure to prepare a herbal composition according to the present invention.

<Example 2> Preparation of wool and hair loss preventing agent according to the present invention

1. Preparation of Mixed Solution A

Di-panthenol 0.2kg, salicylic acid 0.25kg, imidazolidinyl urea 0.1kg, allantoin 0.1kg, disodium EDTA was dissolved in 70L purified water and mixed to prepare a mixed solution A.

2. Preparation of Mixed Solution B

Dimethicone 1kg, methylparaben 0.1kg, elmentol 0.3kg was dissolved in 18kg of ethanol and mixed to prepare a mixed solution B.

3. Manufacture of herbal extracts

As in Example 1, each herbal extract was prepared by mixing licorice extract 0.1kg, Angelica extract 0.66kg, iris extract 0.3kg, Cheongung extract 0.66kg, sewage extract 0.5kg, and Euiin extract 0.3kg.

4. Mixing process

The mixed solution A, B and the herbal extracts prepared in the 1, 2, and 3 steps and 1 kg of propylene glycol were mixed to prepare a wool and hair loss preventing agent according to the present invention.

Comparative Example 1

Comparative Example was prepared in Example 2, except for the herbal composition according to the present invention.

Experimental Example 1 Wool Efficacy Evaluation Experiment

1. Experimental Materials and Methods

  end. Experimental Sample

Samples were prepared using the formulation prepared in Example 2, and the control group was prepared in the comparative example and 3% minoxidil was used as a positive control.

  I. Laboratory animals

As an experimental model, 5 weeks old female and male C57BL6 mice were purchased from Daehan Biolink Co., Ltd. and used for experiments after adapting to the animal breeding environment for 1 week. Animal breeding room was 23 ± 3 ℃, relative humidity 50 ± 10%, lighting time was maintained at 7 am~7pm, 12 hours day and night, and the illumination was 150 ~ 200Lux. Experimental group used 6 animals, 3 each of female and 3 males, and 60 of 10 animals per group. The experimental animals were reared in the mouse cage [Samgwang Co., Ltd.] for isolation, and were freely fed the experimental animal feed (Samyang Feed).

  All. Sample processing

The back hairs of 6-week-old female and male mice were removed using an animal clipper, and sample coating was carried out once daily from Monday to Saturday for 30 days from the day after hair removal. 0.2 ml. All experiments were performed after pooling mice in different embryos, sorting them to homogenous mean weight and variance.

  la. Weight measurement

Body weight was measured every week immediately before application of the sample and at a fixed time until the end of the experiment.

  hemp. Determination of Effect

Experimental animals were photographed by anesthesia with ether from 7 days after the start of the experiment to 4 weeks. The wool effect by the Examples of the present invention, the Comparative Example and the positive control group was determined by the degree of the hair grown on the sample application site (the ratio of the area of hair growth after sample treatment among the back hair removal areas) on the photographs shown in FIGS. 1 and 2. According to the criteria of Table 1, we scored visually and the comparison between each group was statistically processed by Student t-test.

Wool Effect Criteria Hair growth degree scoring Hair does not grow 0 Less than 30% One 30% to 70% 2 More than 70% 3

  bar. Histological analysis

Experiments were performed on the Comparative Example, Example, and Positive Control groups to observe the degree of development of the scalp tissue and to examine the wool effect.

On the 30th day of the experiment, the skin tissues of the example group, the positive control group and the comparative group were extracted using an anatomical apparatus and fixed with formalin. Step by step dehydration with alcohol and xylene and embedded in paraffin, 5μm sections were made using a microtome and paraffin was removed again with alcohol and xylene. After hematoxylin & eosin staining, hair follicle morphology was observed under an optical microscope.

2. Experimental Results

   end. Weight and skin color changes

Changes in body weight tended to decrease slightly in females compared to controls, but there was no significant difference. Changes in skin color occurred quickly in females, and no adverse effects such as skin abnormalities were found.

   I. Wool effect

The results for the wool effect are shown in FIGS. 1 and 2 for males and the results for females are shown in FIGS. 2 and 3.

Wool Effect Scores in Male Mice Comparative example Test group Positive control group (minoxidil) 0 weeks 0 0 0 1 week 0 0 0.8 2 weeks 0 0.7 2.5 3 weeks 0 1.4 2.8 4 weeks 0 2.5 3.0

Wool determination criteria: hair not growing; 0, less than 30%; 1, 30-70%; 2, 70% or more; 3

Judgment score = visual judgment score / object

Wool Effect Scores in Female Mice Control Test group Positive control group (minoxidil) 0 weeks 0 0 0 1 week 0 0.1 0.6 2 weeks 0 0.4 1.9 3 weeks 0.1 1.6 2.9 4 weeks 1.5 2.6 3.0

Wool determination criteria: hair not growing; 0, less than 30%; 1, 30-70%; 2, 70% or more; 3

Judgment score = visual judgment score / object

Example group according to the present invention from 10 to 15 days from the beginning of the test began to change the color of the skin quickly to gray and gradually began to grow blue to grow hair. Hair growth rate was also rapid in females, but not significantly different. In the example group and the positive control group, black hair was found at 1/2 or more on the 20th day of the experiment, but the growth rate of the hair was much lower in the comparative group. In other words, although the change of skin color and hair growth time were slightly slower than those of the positive control group, the hair growth was significantly promoted in both females and males as compared to the comparative control group. Compared with the group, it showed a statistically significant level (P <0.001) of good wool effect.

Therefore, it can be confirmed that the preparation according to the present invention has a hair loss prevention and wool effect.

   All. Histological analysis

Morphological changes of the hair follicles confirm the hair loss prevention and wool effect, as shown in Figures 3a to 3c, 4a to 4c, the Example group is frozen section of the skin tissue of the mouse after 30 days after the start of application As a result, both skin thickness is thicker, hair follicle tissue length is longer, skin depth is located, and hair follicles are formed compared to the comparative and positive control groups. It showed a rapid progress.

In particular, in both male and female mice, the embodiment group according to the present invention has a thicker skin thickness, a longer follicle tissue length, a deeper skin position, and forms a hair follicle than the comparative group and the positive control group. As can be seen, it can be seen that the present invention is a wool and hair loss preventing agent suitable for both men and women.

In addition, in the histological analysis, another embodiment of the present invention is to show that re-hair loss does not occur even if the drug is stopped after hair growth promoting and hair loss prevention treatment.

Therefore, it is confirmed histologically that the wool composition of the present invention has an anti-hair loss and a wool effect.

< Experimental Example  2> hair loss inhibition effect test

In order to confirm the wool effect of the herbal composition according to the present invention confirmed in Experimental Example 1 in the clinical subjects with alopecia were selected to measure the wool and hair loss inhibitory effect and maintenance effect.

Using the formulation according to the present invention prepared in Example 2 and Comparative Example 1 not containing the same, 10 persons of adult male alopecia of 30 to 50 years old or persons having an average number of hair loss of 100 or more per day were selected.

How to use: Apply about 5ml 2 ~ 3 times a day to the hair loss area for 3 months and rub it with fingertips to massage it to be absorbed sufficiently. After 3 months, check hair loss for 2 months. It was.

As for the determination of the effect, the average daily hair loss at trituration, gross findings, and improvement of subjective symptoms were evaluated in five stages according to the scale of Table 4, and the results are shown in Table 5, and 3.0 or more were determined to be effective.

division Hair Loss at Tricycle Subjective symptoms Visual judgment Retention effect 5 A marked decrease A marked decrease A marked decrease Hair growth 4 Reduced severity Reduced severity Reduced severity Status 3 Hardness decrease Hardness decrease Hardness decrease Slight hair loss 2 immutability immutability immutability Hair loss One Exacerbation (hair loss) Exacerbation (hair loss) Exacerbation (hair loss) Pretest State

division Hair Loss at Tricycle Subjective symptoms Visual judgment Retention effect Example 1 4.7 4.5 4.6 4.1 Comparative Example 1 1.3 1.9 2.1 - Positive control group 4.9 4.7 4.6 3.2

As shown in Table 5, it can be confirmed that the preparation according to the present invention is very excellent in preventing hair loss as well as promoting hair growth.

In particular, the herbal composition according to the present invention grows somewhat slower hair loss prevention effect than the minoxidil formulation of the positive control, but in the maintenance effect is applied to the formulation of the present invention after the hair loss maintenance effect as well as the hair loss prevention effect than the minoxidil formulation If not, it can be seen that the hair loss prevention effect is very significant.

< Experimental Example  3> skin irritation probe test

Skin irritation studies were tested to determine the extent of side effects when the formulations according to the invention were applied to the scalp.

1. Test Method

  end. Test animals

Six male New Zealand White rabbits have a temperature of 23 ± 3 ℃, a relative humidity of 55 ± 15 ℃, a ventilation frequency of 10 to 20 times / hour, an illumination time of about 8 hours to 8 pm, and about 150 to 300Lux Breeding was selected in the environment set to.

  I. Application of Example 2 Formulations According to the Invention

24 hours before the application of the preparation prepared in Example 2 according to the present invention, the hair of the rabbit was depilated about 10 cm in width and width, and then the divided skin was divided into left and right, and the left and right sections were used as the control compartment, and the applied compartment. The normal skin or abrasion skin of the control and control sections were divided diagonally from each other, and two normal (nonchalginal) skins and two abrasion skins of 2.5 cm × 2.5 cm were marked with oil-based pens. The abrasions and skin are made by scratching the skin so that the epidermis is damaged but not the dermis. As a method of application, 0.5 ml of 2.5 cm × 2.5 cm is applied to the applied compartment skin so as to cover the gauze to which the formulation prepared in Example 2 according to the present invention is applied so that the formulation is in good contact with the skin, and the formulation is evaporated on the gauze. The tape was wound with a tape of low permeability and low irritation to maintain for 24 hours.

  C. Test Methods and Test Items

    1) General symptoms observation

All animals were observed for symptoms at least once daily.

    2) Determination of skin reaction

Skin Response Evaluation Criteria

  ① formation of erythema and skin

reaction Degree No erythema 0 Very mild erythema (only visible to the naked eye) One Obvious erythema 2 Slightly severe erythema 3 Severe erythema and mild skin formation 4

  ② edema formation

reaction Degree No edema at all 0 Very mild edema, barely visible to the naked eye One Mild edema (if the swelling is obvious and the margins are clearly distinguished) 2 Normal edema (when it swells about 1mm) 3 Severe edema (swelling more than 1 mm and extended outside the exposed area) 4

    3) Evaluation of skin reaction results

Using the score of skin reactivity, the average value was calculated by adding the erythema score and the edema score at 24 hours and 72 hours after the application of the above formulation, and the skin irritation was evaluated by this value. At this time, the irritation of the formulation was evaluated in consideration of the general symptoms observed during the test period in addition to the primary skin irritation index.

1st skin irritation index ^{1 )} division 0.0-0.5 Non-irritant 0.6-2.0 Mild irritant 2.1-5.0 Severe Irritation 5.1-8.0 Strong irritant

¹⁾ PII (Primary Irrtation Index): The primary stimulus index divided by 4

   la. result

    1) General symptoms observation

No dead animals were observed during the entire trial.

    2) Skin reaction

① formation of erythema and skin

  In all six cases, no symptoms were observed at the normal site of the application section for 24 hours. In the area of abrasions, mild redness was noted in 4 cases and mild redness in 2 cases. In the control compartment, all six cases showed mild erythema only in the elevated area.

No abnormality was observed at the normal site of the application compartment at 72 hours. Minor erythema was observed in only one case. No abnormalities were observed in all six cases in the control compartment.

② edema

   Edema was not observed in normal and abrasions of the control and applied compartments during the entire observation period.

    3) Determination of irritation

As a result of determination of locality based on skin response, the primary skin irritation index (P.I.I) was non-irritating at 0.25 for the control compartment and 0.46 for the applied compartment.

Therefore, the formulation according to the present invention will be said to be a formulation that can be safely applied to the scalp without irritation.

Therefore, the present invention can prepare a wool and hair loss preventing agent based on the composition extracted from the herbal medicine containing licorice, Angelica, iris, cheonggung, sewage and Euiin, the herbal composition according to the present invention has the effect of promoting hair growth and preventing hair loss Has

In particular, the present invention solves the problem that occurs in conventional wool or hair loss preventing agents, that is, the problem of re-hair loss by stopping the drug administration, the herbal composition according to the present invention after promoting the hair growth and hair loss prevention effect of the present invention Even without applying the agent, it has a continuous hair growth and hair loss prevention effect.

Claims (2)

Wool and hair loss prevention agent containing licorice, Angelica, irises, horoscopes, sewage and uiyi herb as main ingredients. 2. The wool and hair loss preventing agent according to claim 1, wherein the prevention of wool and hair loss is that re-hair loss does not occur even if the administration is stopped after the treatment.

Images