KR20070027678A - The method for breeding a fish culture using deep seawater - Google Patents

Abstract

Provided are a method for breeding marine product such as an eel by using deep sea water, and a product of the marine product raised by the method which is free from fishy smell, is reduced in fats, is lowered in the content of cholesterol and is increased in the content of amino acids and minerals. The method comprises the steps of filtering deep sea water with a diatomite filter of 0.3-0.8 micrometers to remove the impurities; drying Phellinus linteus to the water content of 8% or less and pulverizing it to 1,000-2,000 mesh; heating 10 L of the filtered deep sea water at 40-60 deg.C for 3-5 days to separate salt and water; cooling the salt-removed deep sea water at 25-35 deg.C; mixing glucose, the obtained deep sea water and the pulverized Phellinus linteus in a ratio of 1:10:15; adding 5 g of amylase based on 1 kg of the mixture and fermenting the obtained one at 20-30 deg.C for 3-7 days; filtering the fermented one and freeze-drying it at -30 to -20 deg.C; heating 100 L of the deep sea water of the first step at 40-60 deg.C for 3-5 days to separate salt and water; heating the salt-removed deep sea water at 50-70 deg.C to concentrate it until the salt concentration reaches 40%; adding 5-10 g of the freeze-dried powder based on 100 L of the concentrate; mixing feed and the concentrated solution in a ratio of 5:1 to 10:1; and feeding the mixture to fish for 30-90 days.

Description

The method for breeding a fish culture using Deep Seawater}

The present invention relates to aquaculture methods using deep sea water, and more specifically, by using deep sea water and fermented mushrooms, there is no fishy smell and odor peculiar to eel, and there is almost no fat and juicy meat. It relates to a farming method that can produce high-quality eel with tenderness and tenderness, reduced fat and cholesterol, increased various minerals and amino acids, and improved taste and nutrition.

      Deep sea water is deep sea water that is more than 200 meters deep without sunlight. Rigorous deep water means seawater resources originating in Greenland and circulating the Atlantic, Indian and Pacific Oceans every 2000 years. The oceans do not stay in one place but are constantly circulating throughout the earth. When the waters that circulate arrive at Greenland's glaciers, they become very cold and become very heavy. This increased specific gravity gradually goes down to a depth of 200m or less, and the temperature drops rapidly to about 2 ℃. This cold water does not actively mix with the warmer water near the upper surface of the water, but exists as borders with each other like water and oil. The water that goes down into the deep sea is called deep sea water. Deep water is not polluted and is rich in nutrient inorganic salts. Shallow seawater can cause photosynthesis, organic growth, and air and land contaminants to easily enter under sunlight, but deep ocean waters will remain pure because these organics and contaminants can't reach a depth of 200 m. Can be. Deep water also circulates through the earth as the seawater flows, and the circulation is so slow that it usually takes around 2000 years to circle the earth. During these long years, the water temperature is constant at nearly 2 ° C while slowly circling the surface of the earth, and it is rich in inorganic nutrients such as nitrogen, phosphorus, and silicon. Interested in deep ocean waters in the United States and Japan from 30 to 40 years ago, it has recently been in the spotlight as its utilization and importance increases in various fields such as fisheries, food, beverage, cosmetics and medicine. In Korea, the Ministry of Maritime Affairs and Fisheries began research on the development of deep water from offshore Goseong-gun, Gangwon-do since 2001, and the government plans to build a marine deep-water pilot industrial complex by 2005 with an investment of 50 billion won.

     The situation is a mushroom that grows in the trunks of apple, mulberry, and oak trees in the Chinese Medicine Dictionary (中藥 大 辭典, 上海 科學 技術), and the names of tinnitus, Sangsin, Hogoan, etc. Have In the New Nongbocho, the difference is recorded in the section of Morus alba L. (,), which is the Mingdae Ishijin. ) Are classified into the terms of Mok-I in the Herbal Wood (本草綱目), and recorded as Different, Sangyu, Ivory, Sang-sang, and Situation. In the agreement, it is classified into difference, situation and so on. Wood muds are Phellinus linteus (Berk. Et Curt) Aoshima, P. igniarius (L. exFr.) Quel, P. yucatensis (Murr.) Imazek, Fomes yucatensis Murrill., Phropolyporus yucatensis Murr. Although it is named in various ways, etc., Phellinus igniarius (Lex Fr.) Quel, which grows in broadleaf trees such as ancestors, sheep, and two people, is widely interpreted. Wood mud mushroom, which is the scientific name of the situation mushroom and is a true situation, grows only in the mulberry tree, unlike the horse mud mushroom. The scientific name is Phellinus linteus (Berk. Et Curt) Aoshima (原色 日本 菌類 圓 鑑) , 1975).

       Woody mud mushrooms grow on the stump of the mulberry tree, and their shape is initially called yellow mud lumps. . The upper part of the tongue-like shape is slightly different depending on the variety of the situation, but it may be mud-like or blackish like the skin of persimmon.

       The pharmacological action of woody mud mushrooms is known to enhance immune function when chemotherapy is performed after resection of liver cancer including gastric cancer, esophageal cancer, duodenal cancer, colon cancer, and rectal cancer. It also activates and detoxifies uterine and bleeding, dysmenorrhea, intestinal bleeding, intestinal and gastrointestinal functions.

       Ikegawa et al. (Gann, 59, 155, 1968) reported that the woody mud mushrooms had higher tumor inhibition than cloud mushrooms (Coriolus versicolor) and shiitake mushrooms (Lent inusedodes). 426, 1976) reported an anticancer activity of 96.7%. Chung et al. (Journal of Pharmaceutical Sciences, 38, 158, 1994) reported that the protein polysaccharide of mycelium effectively suppressed Sarcoma 180 ascites and solid cancer, and Kim et al. (Arch, Pharm. Res., 17, 337, 1994). As a result of analyzing polysaccharides having antitumor activity, glucose is mainly composed of glucose, and in addition to galactose, mannose and ara

It was reported that it consists of Arabinose et al. And Gong et al. (Journal of Korean Journal of Pharmacognosy, 22, 233, 1991) showed that the hydrothermal extract of woody mud (situation) mushroom mycelium showed anticancer activity by acting on NK cell function and enhancing the host's nonspecific immune function. LD50 reported to be safe at more than 1,500 mg / kg.

As such, woody mud mushrooms have been reported to have excellent anticancer activity and immune enhancing effect.

   Eel is a food that is consumed as a diet containing high nutrients. However, these eel not only smell fishy and peculiar smell, but also greasy and sensational, high cholesterol, high quality nutritional ingredients, despite the narrow range of consumers.

Therefore, the technical problem to be achieved by the present invention is to treat the deep sea water and the situation mushroom by mixing in the feed and cultivate the eel peculiar to the eel without the smell of peculiar meat tender and sued, juicy, causing the adult disease To reduce fat, cholesterol, and various minerals and amino acids to provide a way to raise eel that can help health.

In order to achieve the above technical problem, the present invention relates to aquaculture method using marine deep water, (a) removing impurities by filtration with a diatomaceous earth filter of 0.3 ~ 0.8 MICRON of deep sea water; (b) drying the mushrooms within 8% of moisture content and pulverizing them at 1000 to 2000 mesh; (c) 10 liters of deep sea water from which the impurities are removed are heated at 40 ° C. to 60 ° C. for 3 to 5 days at low temperature. Separating salt and water; (d) Separating the salt in the above step and cooling the remaining deep sea water to 25 ℃ to 35 ℃; (e) Glucose: deep sea water produced in the step: crushed situation mushroom Mixing 1:10:15; (f) adding fermentation at a rate of 5 g of amylase per kg of the mixed material and fermenting at 20 ° C. to 30 ° C. for 3 to 7 days; (g) filtering the fermented material and then lyophilizing at −20 ° C. to −30 ° C. to powder; (h) separating salt and water by heating 1000 liters of deep sea water in step (a) at 40 ° C. to 60 ° C. for 3 to 5 days at low temperature; (i) separating the salt from the deep sea water at 50 ° C. Heating to a temperature of from 70 ° C. to concentrating the salinity to 40%; (j) adding 5g to 10g of (g) the lyophilized powder per 100 liters of the concentrate; (k) aquaculture feed: preparing a mixture of the concentrate in a ratio of 5: 1 to 10: 1; (l) feeding the mixed feed to aquaculture fish for 30 days to 90 days; and a method for farming aquatic products of high quality, including.

 Eel cultured according to the culture method of the present invention has no fishy smell and peculiar smell peculiar to the eel, and there is little fat, so it is light and tender, tender and succulent, rich in minerals, low fat and high protein, and general eel. In comparison, high-quality eel, which has a 27% reduction in cholesterol, is enhanced in taste and nutrition, contributing to national health.

      Hereinafter, aquaculture method using the deep sea water according to the present invention will be described in detail.

First, impurities are filtered by diatomaceous earth filter of 0.3 ~ 0.8 MICRON of deep sea water. The mushrooms are dried within 8% moisture content and pulverized to 1000 to 2000 mesh. 10 liters of deep sea water from which impurities are removed are heated at low temperature at 40 ° C to 60 ° C for 3 to 5 days to separate salt and water. The salt is separated and the remaining deep sea water is cooled to 25 ° C. to 35 ° C., and the glucose: salt is separated and the remaining deep sea water: ground mushroom is mixed at 1:10:15. It is added at a rate of 5 g of amylase per kg of the mixed material, fermented at 20 ° C. to 30 ° C. for 3 to 7 days, and then filtered and lyophilized at −20 ° C. to −30 ° C. to powder. 1000 liters of deep salt water, which is not separated from salt, is heated at 40 ℃ to 60 ℃ for 3 to 5 days at low temperature to separate salt and water, and the salt is separated and the remaining deep water is heated to a temperature of 50 ℃ to 70 ℃. Concentrate the salinity to 40%. Aquaculture feed by adding 5g to 10g of lyophilized situation mushroom solids per 100 liters of concentrate: Mix the concentrate in a ratio of 5: 1 to 10: 1, and then feed the mixed feed to aquaculture fish for 30 to 90 days It provides a high-quality aquaculture method comprising the step of.

    As such, the eel cultured according to the culture method of the present invention is almost greasy and has no fishy and odor smell peculiar to the eel, and is juicy, meaty and tender, succulent, rich in minerals, and low fat and high protein. This is a high quality eel with a 27% reduction in cholesterol compared to regular eel. In addition, eel produced by this farming method does not have any smoke or smell when baked in a pan, and it enhances the taste and nutrition to help health.

Hereinafter, the present invention will be described in detail with reference to Examples. However, embodiments according to the present invention may be modified in many different forms, and the scope of the present invention should not be construed as being limited to the embodiments described below. The embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

Example

By the above method, eel meat was obtained by feeding eel 6 months old for 30 days.

Comparative example

 Six-month-old eel was cultivated for 30 days in the usual way to obtain eel meat.

After removing the internal organs of the eel produced according to the Examples and Comparative Examples, each of them was baked in a frying pan without adding anything, such as seasonings, spices, or salt for smelling or flavoring. And the grilled eel of the comparative example was tried to evaluate the satisfaction with the taste and meat quality, the evaluation method according to the four-point method, there is no fishy and odor smell peculiar to the eel, respectively, it is light and is very sued It is fleshy, elastic and very good intake: 4 points, almost no fishy and smelly eel, less oily, relatively light, savory, meaty, relatively dull, elastic and good intake : 3 points, fishy and odor characteristic peculiar to eel, normal oily and slight feeling, meat lacks elasticity slightly Common taste: 2 points, fishy smell and odor peculiar to eel, oily and heavy feeling, no elasticity of meat, very bad intake, disgusting: 1 mark and the average score is shown in Table 1 Indicated.

<If you add nothing to the eel and bake each in a frying pan> division Fishy A peculiar smell flavor Flesh Intake Example 4 3.9 3.7 3.8 4 Comparative example 1.7 1.2 2.1 2.4 2.2

<Comparison Analysis of Eel Ingredients> division General ingredients (per 100g of decorative part) moisture (%) protein (%) Fat (%) Ash content (%) carbohydrate (%) Ca (mg) P (mg) Na (mg) K (mg) Mg (mg) Zn (mg) Example 67.1 18.1 12.5 1.5 0.8 168 221 69 257 33 3.1 Comparative example 66.9 13.9 17.5 1.2 0.5 142 186 62 224 18 1.1

division Amino acid (per 100 g of mg / plant) Isoleucine Leucine Lysine Methionine Cysteine Phenylalanine Tyrosine Threonine Example 775 1231 1350 599 251 755 617 622 Comparative example 715 1125 1287 519 176 698 501 572

<Comparison Analysis of Eel Ingredients>  division Amino acid (per 100 g of mg / plant) Valine Histidine Arginine Alanine Aspartic acid Glutamic acid Glycine Proline Example 765 411 1108 867 1193 2127 701 582 Comparative example 742 387 1002 808 1124 2021 607 507

<Comparison Analysis of Eel Ingredients> division Per 100g of edible Cholesterol (mg) Lipid (g) Total Fatty Acid (g) Saturated fatty acid (g) Unsaturated fatty acid Monounsaturated (g) Polyunsaturated (g) Example 145 12.5 15.2 3.87 7.82 3.51 Comparative example 199 17.5 19.0 5.78 10.1 3.12

Referring to Table 1, the eel produced according to the breeding method of the present invention has no fishy and peculiar smell of smell, has little grease, and is light, very savory, meaty, elastic and very good intake. Able to know. Referring to Table 2 to Table 5 it can be seen that the eel of the embodiment is more protein than the eel of the comparative example, the lipid is significantly reduced, various minerals, amino acids are increased and cholesterol is significantly reduced.

As such, in general, eel is not easy to bake in a frying pan due to the abundance of fat, whereas eel cultured by the aquaculture method of the present invention is less smoked even when roasted in a frying pan, and the eel fishy and the tasting result table as shown in the result table. It has no peculiar smell of smell, is almost greasy, so light, very sued, meaty, elastic and very good intake. In particular, the eel cultured according to the present invention is a high-quality eel rich in minerals, low-fat, high protein, and reduced cholesterol by 27% compared to the general eel, high quality food useful because it increases the taste and nutrition to help health Its role is expected.

Claims (2)

(a) step of removing impurities by filtration with diatomaceous earth filter of 0.3 ~ 0.8 MICRON of deep sea water.    (b) drying the mushrooms within 8% moisture content and pulverizing them at 1000 to 2000 mesh.    (c) separating salt and water by heating 10 liters of deep sea water from which the impurities are removed at 40 ° C. to 60 ° C. for 3 to 5 days at low temperature.    (d) separating the salt in the above step and cooling the remaining deep sea water to 25 ° C to 35 ° C.    (e) glucose: mixing the deep seawater: ground crushed mushrooms produced in the step 1:10:15.    (f) fermentation at 20 ° C. to 30 ° C. for 3 to 7 days by adding a ratio of 5 g of amylase per kg of the mixed material.    (g) filtering the fermented material and then lyophilizing at -20 ° C to -30 ° C to powderize.    (h) separating salt and water by heating 1000 liters of deep sea water of step (a) at 40 ° C. to 60 ° C. for 3 to 5 days at low temperature.    (i) separating the salt and concentrating the salinity to 40% by heating the remaining deep sea water to a temperature of 50 ℃ to 70 ℃.    (j) adding 5 g to 10 g of the (g) step lyophilized powder per 100 liters of the concentrate.    (k) aquaculture feed: preparing a mixture of the concentrate in a ratio of 5: 1 to 10: 1.    (l) aquaculture method of aquatic products using deep sea water comprising feeding the mixed feed to aquaculture fish for 30 days to 90 days Products of aquaculture farmed according to the method of paragraph 1