KR20060109063A - COMPOSITIION FOR INHIBITING 5alpha;-REDUCTASE ACTIVITY AND COSMETIC COMPOSITION FOR INHIBITING SECRETION OF SEBUM CONTAINING EXTRACT OF CHELIDONIUM MAJUS AS ACTIVE INGREDIENT - Google Patents

Abstract

Compositions for inhibiting oversecretion of sebum and alleviating acne comprising the extract of Chelidonium majus as an active ingredient are provided to inhibit 5alpha-reductase activity and production of dihydrotestosterone, and reduce side effects including skin irritation and antibiotic-resistance induction. The cosmetic composition for inhibiting oversecretion of sebum and alleviating acne comprises the extract of Chelidonium majus as an active ingredient, wherein the Chelidonium majus extract is prepared with a solvent selected from water, C1-4 anhydrous or hydrous lower alcohol, acetone, ethylacetate, butylacetate and 1,3-butylene glycol; the amount of Chelidonium majus extract is 0.00001-20 wt.% based on the total weight of the composition; and the composition is formulated as solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation or spray.

Description

Composition for inhibiting 5α-reductase activity and sebum secretion containing Baekgulchae extract as an active ingredient {Compositiion for Inhibiting 5α-Reductase Activity and Cosmetic Composition for Inhibiting Secretion of Sebum containing Extract of Chelidonium majus as Active Ingredient}

The present invention relates to a composition for inhibiting 5α-reductase activity, a cosmetic composition for inhibiting sebum secretion, and a cosmetic composition for acne alleviation.

5α-reductase is present in testosterone-reactive tissues such as sebaceous glands, hair follicles, prostate and epididymis, reducing testosterone, one of the testosterones, to dihydrotestosterone (DHT). As an enzyme involved, the reduction requires NADPH (Nicotinamide-adenine dinucleotide phosphate). Testosterone is involved in adult sexual impulses, skeletal muscle growth, male external genitalia and scrotum growth, and spermatogenesis, while dihydrotestosterone is involved in acne, sebum and prostatic hyperplasia (Diane et al., JID , 775). -778, 1995; Bruchovsky, N et al., JBC , 243, 2112-2121, 1968). Recently, studies to develop acne treatments and sebum secretion inhibitors by using inhibitors of 5α-reductase enzyme have been actively conducted.

In general, sebum is responsible for keeping the skin moisturized and microorganisms in the skin, including the scalp and face, but it causes acne after puberty. In particular, women in their 20s and later cause a lot of problems in terms of beauty, such as skin lingering, makeup lifting and pores widening due to the excessive secretion of sebum. Excess sebaceous secretion is involved in a number of causes, the most important of which is the sebaceous gland secretion is activated by the cells of the sebaceous gland by dihydrotestosterone, one of the hormones involved in activating the sebaceous gland to promote sebum secretion Known.

Acne is an inflammatory disease that occurs in the hair follicle sebaceous glands of the skin, the cause of which is not clearly identified, but generally the following mechanism has been suggested. Excessive secretion of sebum by the testosterone testosterone causes the hair follicle tube to be over-exaggerated, which in turn blocks the secretory exit of the sebum by the hair follicle tube, causing sebum to accumulate in the hair follicle. Accumulation of excess sebum leads to the development of acne bacteria (eg Propionibacterium acnes), which are anaerobic and anaerobic bacteria, which grow inside the sebum by the lipase secreted by acne bacteria. Is converted into free fatty acids, which cause irritation to the skin resulting in inflammation. In addition, acne bacteria are recognized as antigens to stimulate the body's immune response to inflammatory reactions. By this mechanism, papules and inflammatory acne lesions are generated, and even worse, hair follicle walls are destroyed, and tissues are damaged, such as cysts and pustules.

To prevent the development and progression of the acne described above, various drugs in the form of oral or external preparations have been used, for example, anti-androgens that control the secretion of abnormal testosterone, non-steroidal anti-inflammatory drugs that act as anti-inflammatory agents, and Resorcinol, sulfur, benzoyl peroxide, erythromycin, tetracycline and the like are used as antibacterial agents and antibiotics that inhibit the growth of acne bacteria. Recently, acne treatments using retinoic acid and vitamin A derivatives have been commercialized.

However, most of the methods tried to date have many problems such as inhibition of epidermal growth, skin irritation or keratinization, or antibiotic resistance, usability.

In recent years, attempts to control acne bacteria by extracting hypoallergenic antimicrobial substances from various plants have been attempted in the field of cosmetic compositions. However, most of these methods have problems such as low antibacterial activity against acne bacteria and separation and purification of active ingredients. Therefore, the necessity of developing a stable and effective product for acne skin is steadily emerging.

On the other hand, as a patent for the treatment and improvement of acne skin, US Patent No. 6168798 discloses a composition for treating acne containing salicylic acid, US Patent No. 6174892, 5α-reducte such as finasteride (etc.) Disclosed are the use of the inhibitor of the ease of acne treatment. In addition, US Patent 4775663 discloses a composition for treating acne comprising a benzofuran derivative, US Patent No. 5019567 discloses benzoyl peroxide-quaternary ammonium lipolic salicylate (benzoyl peroxide-quaternary ammonium lipophilic Disclosed is a composition for treating acne comprising salicylate).

Throughout this specification, numerous patent documents and articles are referenced and their citations are indicated in parentheses. The disclosures of cited patent documents and articles are incorporated herein by reference in their entirety, and the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly described.

Thus, the present inventors conducted a study to search the inhibitory rate of 5α-reductase activity using 200 kinds of natural plant extracts in order to overcome the weak effects and side effects of the conventional acne treatment, the extract of Baekchulchae The present invention was completed by confirming that the 5α-reductase activity inhibitory effect was excellent, the sebum secretion inhibitory effect and acne relieving effect were very excellent.

Accordingly, an object of the present invention is to provide a composition for inhibiting 5α-reductase activity containing Chelidonium majus extract as an active ingredient.

In addition, another object of the present invention to provide a sebum secretion cosmetic composition for inhibiting sesame seed extract as an active ingredient.

In addition, another object of the present invention to provide a cosmetic composition for acne relief containing baekchulchae extract as an active ingredient.

Other objects and advantages of the present invention will become apparent from the following detailed description and claims.

According to an aspect of the present invention, the present invention provides a composition for inhibiting 5α-reductase activity, which contains Chelidonium majus extract as an active ingredient.

According to another aspect of the present invention, the present invention provides a cosmetic composition for inhibiting sebum secretion containing baekchulchae extract as an active ingredient.

According to another aspect of the present invention, it provides a cosmetic composition for acne relief containing baekchulchae extract as an active ingredient.

"Acne" to which the composition of the present invention is applied includes any skin disease in the art, or any skin disease that is invented through a mechanism similar to acne, for example, collective acne, acne caused by bromide, general acne, Congoblate acne, acne from cosmetic use, acne dtergicans, adolescent acne, acne fulminans, acne furunculoid, acne caused by halogen, callous acne, Keloid acne, physical acne (mechanical acne), drug acne, necrotic acne, lip acne, acne neonatorum, acne oil, papule acne, pomade acne, Premenstrual acne, acne rosacea, swelling acne, tropical acne, addictive acne venenata, and vulgar acne It is.

The composition for inhibiting 5α-reductase activity, the cosmetic composition for inhibiting sebum secretion, and the acne-relieving cosmetic composition provided by the present invention contain baekgulchae as an active ingredient.

Baekgulchae is a perennial herbaceous perennial plant of Chelidonium majus , relieves abdominal pain and dysentery caused by acute gastroenteritis, gastric and duodenal ulcer, cholecystitis, jaundice hepatitis, skin ulcers, tuberculosis, scabies and ringworm. Used. Treats customary quadriplegia pain. In addition, a patent has been registered for a polysaccharide composition excellent in anticancer activity, immune enhancement and hematopoietic properties extracted from Baekchulchae (Korean Patent Registration No. 310059).

In the present invention, baekchulchae extract means that it is obtained by extracting from various organs or parts (eg leaves, flowers, stems, branches and bark, etc.) in the ground part of celandine grass, preferably the leaves, flowers, stems of celandine grass Refers to extracts obtained from the ground, including both eggplant and bark.

The white bran extract of the present invention may be prepared according to conventional methods known in the art, that is, using a conventional solvent, under conditions of conventional temperature and pressure.

Baekgulchae extract, an active ingredient of the composition of the present invention, is extracted using a solvent selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3 butylene glycol Preferably, ethyl alcohol, even more preferably 70% ethyl alcohol. A suitable amount of the extracting solvent is 1-10 times the dry weight of the baekjachaegi, more preferably 5-8 times, most preferably 6 times.

White bran extract of the present invention is prepared by a process comprising the following steps: (a) 1-10 times water, 1-4 anhydrous or hydrous lower alcohols, acetone, ethyl acetate, butyl in dried white buckwheat Depositing by adding a solvent selected from the group consisting of acetate and 1,3 butylene glycol; (b) filtering the solvent-added white bran of step (a) and concentrating in a vacuum concentrator; And (c) filtering and drying the components concentrated in step (b).

According to one specific embodiment of the present invention, the above-described extraction process is carried out as follows: First, the white buckwheat serving as a raw material is washed, dried and powdered, and then 70% ethyl alcohol is 6 times the dry weight of the white buckwheat. Add by volume and allow to deposit for 5 days. Subsequently, the filtrate was filtered to 300 mesh, and then concentrated using a vacuum vacuum concentrator to prepare white bran extract.

On the other hand, the extract of Baekgulchae of the present invention includes not only the extract by the aforementioned extraction method, but also an extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). Fractions are also construed as being included in the Yeonderok extract of the present invention.

Baekgulchae extract of the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze drying or spray drying.

According to a preferred embodiment of the present invention, the content of Baekchulchae extract of the present invention is 0.00001-20% by weight, based on the total weight of 5α-reductase activity inhibiting composition, sebum secretion cosmetic composition and acne relief cosmetic composition, Preferably it is 0.0001-10 weight%, Most preferably, it is 0.001-7.0 weight%. At this time, when the total weight of the extract of Baekchulchae is less than 0.00001%, the effect is less likely to occur, when 20% or more is likely to cause irritation to the skin, it may have a great effect on the stabilization of the formulation. In a preferred embodiment of the present invention was prepared a cosmetic composition containing 0.001-3% Baekchulchae extract, it was confirmed that the sebum secretion inhibitory effect and acne inhibitory effect is excellent.

The composition for inhibiting 5α-reductase activity including Baekchulchae extract of the present invention can be used for the purpose of inhibiting sebum secretion and acne alleviation, and the composition can be applied to the face, scalp or the back part with a lot of sebum secretion. The present invention is not limited to the above site and may be applied to any site in which sebaceous glands are developed.

Suitable dosages of the compositions of the present invention vary depending on factors such as the formulation method, mode of administration, age, weight, sex of the patient, severity of acne, food, time of administration, route of administration, rate of excretion, and reaction sensitivity. The skilled practitioner can readily determine and prescribe a dosage effective for the desired treatment or prophylaxis. Preferably, the dosage of the pharmaceutical composition of the present invention is approximately 0.01 mg / kg-100 mg / kg in a daily dosage.

The composition of the present invention exhibits an excellent inhibitory effect of 5α-reductase activity even if only the extract of Baekchulchae as an active ingredient, and may exhibit an inhibitory effect of excessive sebum secretion and an acne relieving effect, but does not irritate the skin, but does not irritate the skin. It may be prepared by further adding other ingredients which may exhibit an ductase activity inhibiting effect.

The extract of Baenggulchae of the present invention may be administered in various parenteral formulations during actual clinical administration, and when formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, surfactants, etc., which are commonly used, may be used. It is prepared by. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. As a suppository base, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerol, gelatin, and the like may be used.

In addition, the cosmetic composition of the present invention contains the ingredients commonly used in cosmetic compositions in addition to the extract of baekryeokchae as an active ingredient, such as conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings, and Carrier.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion (skin), nourishing lotion (milk lotion), nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder. .

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, and especially in the case of spray, additionally chlorofluorohydrocarbon, Propellant such as propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystals are used as carrier components. Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Baekgulchae extract of the present invention is excellent in inhibiting 5α-reductase activity effectively inhibits the production of dihydrotestosterone (Reference: Experimental Example 1), and effectively inhibits the development of scrim, the primary lesion of acne (Reference: Experiment Example 2). In addition, the cosmetic composition containing the extract of Baekchulchae having such an effect as an effective ingredient not only effectively suppresses excessive secretion of sebum (Reference: Experimental Example 3), but also has an excellent acne-improving effect (Reference: Experimental Example 4).

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

Example 1 Preparation of Baekchulchae Extract

100 g of dried white oysters were powdered and placed in 0.6 L of 70% ethyl alcohol, and deposited at room temperature for 5 days, filtered through 300 mesh, and left for 1 week, and then the precipitate was filtered through Whatman 5 filter paper. Then, the resultant was concentrated using a vacuum vacuum concentrator and completely dried using a lyophilizer to obtain a dry weight of 13.50 g of white bran extract.

Example 2 Preparation of Baekchulchae Extract

100 g of dried white oysters were powdered and placed in 0.6 L of 95% ethyl alcohol, which was deposited at room temperature for 5 days, filtered through 300 mesh, and left for 1 week, and then the precipitate was filtered through Whatman 5 filter paper. Then, the resultant was concentrated using a vacuum vacuum concentrator and completely dried using a freeze dryer to obtain a dry weight of 15.50 g of white bran extract.

Example 3-17 Preparation of Baekchulchae Extract

The extract of Baekgulchae was prepared in the same manner as in Examples 1 and 2 except for using the extraction solvent of Table 1 below.

extract Extraction solvent Dry weight of the final extract (unit: g) Example 3 10% ethyl alcohol 11.1 Example 4 20% ethyl alcohol 12.4 Example 5 40% ethyl alcohol 13.4 Example 6 50% ethyl alcohol 14.8 Example 7 60% ethyl alcohol 14.0 Example 8 80% ethyl alcohol 15.2 Example 9 90% ethyl alcohol 16.5 Example 10 Methyl alcohol 15.3 Example 11 n-propanol 10.5 Example 12 Isopropanol 15.6 Example 13 2-butanol 15.2 Example 14 Acetone 10.5 Example 15 chloroform 13.2 Example 16 Ethyl acetate 10.3 Example 17 Butyl acetate 9.6

Experimental Example 1 5α-Reductase activity inhibitory effect test

The following experiment was conducted to determine whether Baekchulchae extract inhibited the activity of 5α-reductase, an enzyme that catalyzes the dihydrotestosterone known to cause sebum secretion and acne production.

<Production of enzyme solution>

A 24-hour fasted Sprogue-Dawley rat (7-8 weeks of age) was killed with Diethylether, followed by obtaining Liver and ice-cold Crab's-Ringer phosphate Perfusion with buffer (Krebs-Ringer phosphate buffer), pH 7.2. Five times of ice-cold Tris-HCl buffer (pH 7.2) was added thereto and homogenized, and centrifuged at 900 x g for 10 minutes. The supernatant obtained by centrifugation was centrifuged at 5,000 x g for 10 minutes, and the supernatant obtained by centrifugation at 9,000 x g for 10 minutes was used as the enzyme solution. The enzyme solution was cryopreserved at -80 ° C.

<Measurement of 5α-Reductase Inhibition Rate>

1.0 ml of Tris-HCl buffer (pH 7.2), 0.3 ml of testosterone (500 µg / ml), white bran extract prepared in Example 1 at a concentration of 0.0001, 0.001, 0.01, 0.1 1.0 and 2.0% and 0.2 ml prepared above After mixing 1.0 mL of the enzyme solution, 0.5 mL of NADPH (0.77 mg / mL) was added to initiate the reaction, and the reaction was carried out at 37 ° C for 30 minutes. After 5 ml of dichloromethane was added to stop the reaction, 0.5 ml of an internal standard (p-hydroxybenzoic acid n-hexyl ester, 0.1 mg / ml) was added thereto, shaken for 10 minutes, and centrifuged at 3,000 rpm for 10 minutes. . The centrifuged supernatant was removed to leave a dichloromethane layer, and 5 ml of methanol was added to the sample for high performance liquid chromatography (HPLC).

The inhibition rate of 5α-reductase was calculated by measuring the amount of testosterone in each experimental group using the HPLC method and substituting the following equation (1), and the values are shown in Table 2.

HPLC conditions: MeOH: water (75:25), 0.8 mL / min, UV detector (254 nm)

cont 0: Do not add white bran extract, but do not cause reaction by adding dichloromethane before adding NADPH

cont 1: the reaction was carried out without the addition of white bran extract

White Oyster Extract (%) 5α-reductase inhibition rate (%) 0.0001 19.3 0.001 20.1 0.01 33.4 0.1 43.2 1.0 67.62 2.0 71.31

Through Table 2, the extract of Baekgulchae of the present invention was found to inhibit the activity of 5α-reductase to about 19.3% -71.31%, and inhibit the activity of 5α-reductase depending on the treatment concentration. Therefore, it can be seen that the extract of Baengkchaechae, which inhibits 5α-reductase activity, has an effect of suppressing the amount of dihydrotestosterone, a male hormone.

Experimental Example 2: Slab Inhibition Test

Treating a cotton-causing agent in the rabbit's ears will cause the cotton to grow after one week, but the larger cotton usually has the same pattern as before acne inflammation. As the scalp grows, there is an excess of sebum in the pores, which causes the pores to become larger than normal. Thus, scalp generation experiments are somewhat different from acne effects in humans, but are the experimental models that most closely resemble acne at present. Thus, the following experiment was carried out to determine whether the extract of Baekchulchae prepared in Example 1 has a cotton cloth suppression ability.

Applying 200 μl of 50% oleic acid / paraffin oil, which is a cotton-causing substance, to the rabbit's ears once a day to induce cotton cloth, and the extract of Baekgulchae prepared in Example 1 is a cosmetic raw material and does not cause cotton cloth. Dissolved in 1% in 3-BG (butyleneglycol), 200 μl was applied twice a day. After treating the oleic acid / paraffin oil to induce the cotton cloth, the experiment was performed by setting the group without the baekchulchae extract as a control. The number of cotton cloths was based on the size of more than 0.2 mm. After removing the keratin by cutting the tissue of 0.8 mm section of the scalp generation site, the size of the scrim was measured by an optical microscope and the results are shown in Table 3. Table A-E of the following Table 3 measures the size of the cotton cloth by randomly selecting six of the well-developed cotton cloths among the whole cotton cloths in order to measure the size of the cotton cloth.

Treatment group The number of animals Cotton catcher Measured cotton cloth size A B C D E Average Oleic Acid Treatment Group (Control) n = 3 16 5 4 6 3 4 4.4 4.53 4 4 5 6 4 4.6 4 5 4 4 6 4.6 Oleic acid and 1% white bran extract treatment group (experimental group) n = 3 7 2 3 2 2 3 2.4 2.47 2 2 4 2 3 2.6 3 3 2 2 2 2.4

As shown in Table 3, the extract of Baekgulchae of the present invention effectively suppressed the generation of cotton cloth by oleic acid treatment. In other words, through the results of less number and size of cotton cloth by the treatment of baekchulchae extract, it was predicted that baekchulchae extract inhibits the progression to acne by inducing less secretion of sebum.

Through the above experiment, it was indirectly confirmed that Baekchulchae extract can alleviate acne by inhibiting sebum secretion by inhibiting the activity of 5α-reductase that causes sebum secretion. Thus, in order to directly check whether the extract of sesame bran extract has sebum secretion and acne relieving effect through the following prescription examples, the prescription examples of cosmetics containing the extract of baekchulchae are presented, but not all prescriptions are limited thereto.

Prescription example 1: flexible lotion

Formulation examples of the flexible lotion in the cosmetic composition containing Baekchulchae extract (Example 1) is as follows.

ingredient Content (% by weight) White bran extract 2.0 1,3-butylene glycol 6.0 glycerin 4.0 PEG 1500 1.0 Sodium hyaluronate 1.0 Polysorbate 20 0.5 ethanol 8.0 Preservative, pigment Quantity Benzophenone-9 0.05 incense a very small amount Purified water Remaining amount Sum 100

Prescription 2: Nutritional lotion

Prescription examples of nutritional lotion in cosmetics containing Baekgulchae extract (Example 1) are as follows.

ingredient Content (% by weight) White bran extract 0.001 1,3-butylene glycol 4.0 glycerin 4.0 Stearyl alcohol 0.8 Glyceryl Stearate 1.0 Triethanolamine 0.13 Tocopheryl acetate 0.3 Floating paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Solbitan Sesquioleate 0.5 Carboxyvinyl Polymer 1.0 Preservative, pigment Quantity incense Quantity Purified water Remaining amount Sum 100

Prescription 3: Nutritional Cream

The prescription example of the nutrition cream in cosmetics containing Baekchulchae extract (Example 1) is as follows.

ingredient Content (% by weight) White bran extract 0.1 Meadowfoam oil 3.0 Stearyl alcohol 1.5 Stearic acid 1.5 Glyceryl Stearate 1.5 Floating paraffin 10.0 Beeswax 2.0 Polysorbate 60 2.5 Solbitan Sesquioleate 0.6 Squalane 3.0 1,3-butylene glycol 3.0 glycerin 5.0 Triethanolamine 0.5 Tocopheryl acetate 0.5 Preservative, pigment Quantity incense Quantity Purified water Remaining amount Sum 100

Prescription Example 4: Massage Cream

A prescription example of a massage cream in cosmetics containing Baekchulchae extract (Example 1) is as follows.

ingredient Content (% by weight) White bran extract 2.0 1,3-butylene glycol 6.0 Sodium magnesium silicate 0.3 glycerin 4.0 Triethanolamine 0.15 Beeswax 2.0 vaseline 5.0 Glyceryl Stearate 1.5 Tocopheryl acetate 0.1 Polysorbate 60 2.0 Solbitan Sesquioleate 0.8 Stearyl alcohol 2.0 Liquid paraffin 40.0 Preservative, incense Quantity Purified water Remaining amount Sum 100

Prescription 5: Pack

Prescription examples of the pack of cosmetics containing Baekgulchae extract (Example 1) are as follows.

ingredient Content (% by weight) White bran extract 3.0 1,3-butylene glycol 1.0 glycerin 2.0 PEG 4000 1.5 Polyvinyl alcohol 12.5 ethanol 7.0 Cured Castor Oil 0.8 Cellulose gum 0.3 Preservative, pigment Quantity incense Quantity Purified water Remaining amount Sum 100

Prescription 6: Essence

Prescription example of the essence of the cosmetics containing Baekgulchae extract (Example 1) is as follows.

ingredient Content (% by weight) White bran extract 1.0 glycerin 10.0 1,3-butylene glycol 5.0 PEG 1500 2.0 Allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Hydroxyethyl cellulose 0.1 Sodium hyaluronate 8.0 Carboxyvinyl Polymer 0.2 Triethanolamine 0.18 Octicdodeceth-16 0.4 ethanol 6.0 Preservative, fragrance, coloring Quantity Purified water Remaining amount Sum 100

Comparative Example 1: Essence containing no extract

Examples of the essence of cosmetics containing no extract of Baekgulchae are as follows.

ingredient Content (% by weight) White bran extract - glycerin 10.0 1,3-butylene glycol 5.0 PEG 1500 2.0 Allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Hydroxyethyl cellulose 0.1 Sodium hyaluronate 8.0 Carboxyvinyl Polymer 0.2 Triethanolamine 0.18 Octicdodeceth-16 0.4 ethanol 6.0 Preservative, fragrance, coloring Quantity Purified water Remaining amount Sum 100

Comparative Example 2: Essence Containing Succulent Extract

An example of an essence prescription in cosmetics containing Euphorbia Lathyris extract (Korean Patent Registration No. 310613), which does not contain Baekchulchae extract and is known to have sebum secretion efficacy, is as follows.

ingredient Content (% by weight) Succulent Extract 1.0 glycerin 10.0 1,3-butylene glycol 5.0 PEG 1500 2.0 Allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Hydroxyethyl cellulose 0.1 Sodium hyaluronate 8.0 Carboxyvinyl Polymer 0.2 Triethanolamine 0.18 Octicdodeceth-16 0.4 ethanol 6.0 Preservative, fragrance, coloring Quantity Purified water Remaining amount Sum 100

Experimental Example 3: Sebum secretion inhibitory effect

In order to check whether the cosmetics containing Baekchulchae extract have the effect of inhibiting sebum secretion, the following experiment was performed by dividing A, B, C into three groups of 20 men and women with high sebum secretion. Group A essence containing baek chaechae extract (prescription example 6), group B essence except the baek chaechae extract in the formulation example (Comparative Example 1), group C essence containing the water extract (Comparative Example 2 ) Were used every morning and evening for four weeks after washing. Sebum measurement was measured for 30 minutes after washing the skin in a constant temperature, a constant humidity room, and then sebum was measured on the forehead using a sebumeter. The amount of sebum was set to the initial value before using the cosmetic. The amount of sebum in the forehead and cheeks was measured once a week for 4 weeks while continuously using an essence (Formulation 6) containing Baekgulchae extract. In comparison with the initial value, it was determined whether the essence containing baekchulchae extract sebum inhibitory effect, the results are shown in Table 12.

week Sebum amount (g / ㎠ / h) Essence with Baekgulchae Extract (Prescription 6) Essence free of white buckwheat extract (Comparative Example 1) Essence containing extracts from Sokja (Comparative Example 2) Initial value 132.4 ± 21.3 141.3 ± 28.1 135 ± 12.9 1 week 127.7 ± 15.7 131.7 ± 22.7 123 ± 22.4 2 weeks 125.6 ± 13.1 135.7 ± 18.8 121 ± 11.1 3 weeks 114.7 ± 15.3 132.4 ± 20.3 115 ± 12.5 4 Weeks 102.8 ± 14.9 134.4 ± 15.7 115 ± 27.8

As shown in Table 12, the essence containing baekchulchae extract effectively reduced sebum secretion over time. In addition, the essence containing Baekchulchae extract was confirmed to have a better effect compared to Comparative Example 2 containing the water extracts known to have a sebum secretion inhibitory effect. Therefore, the extract of Baekgulchae of the present invention can effectively prevent the occurrence of acne due to excessive secretion of sebum by effectively reducing sebum secretion.

Experimental Example 4: Acne Improvement

The essence of Formulation Example 6 was applied to the entire face for four weeks in the morning and evening for 10 men and women with acne. Determination of the acne improvement effect is to evaluate the pre-test state to 1-3 points by reference to the following criteria according to the degree of the person who participated in the experiment to evaluate the acne healing effect and the results are shown in Table 13.

<Evaluation before the test>

1 = some acne 2 = some acne 3 = severe acne

<Acne effect judgment>

-: Almost no effect +: Slight effect

++: Relaxed a lot +++: Completely healed

Subject Pretest State Effect after 2 weeks After 4 weeks Subject 1 2 + + Subject 2 One + ++ Subject 3 One - + Subject 4 2 - - Subject 5 2 + ++ Subject 6 2 + + Subject 7 3 ++ +++ Subject 8 2 - ++ Subject 9 3 + + Subject 10 2 + ++

As can be seen in Table 13, after four weeks of using the essence containing baekryekchae extract was confirmed that the improvement effect of acne in the majority of subjects.

Experimental Example 5: Skin Primary Irritation Test

In order to identify the skin irritation problem, which is a problem of the acne treatment composition, the skin irritation properties of the cosmetic formulations of Formulation Examples 1-6 and Comparative Example 1 containing white bran extract were examined. Thirty healthy males and females were placed on a 5 × 20 cm area of the lower arm of the arm for 24 hours in a fixed amount (approximately 0.1 g), and then removed. The change in skin condition was read according to the criterion, and the stimulation rate was calculated by substituting the result into the following equation and is shown in Table 14 below.

<Decision Criteria>

-: No erythema or unusual phenomenon

+-: Slightly reddish

+: Significantly redder than the surroundings

++: reddens and swells more heavily

Experimental group Number of test subjects Judgment result Stimulation rate (%) ++ + +- - Prescription Example 1 30 - - One 29 1.1 Prescription Example 2 30 - - One 29 1.1 Prescription Example 3 30 - - 2 28 2.2 Prescription Example 4 30 - - 2 28 2.2 Prescription Example 5 30 - - One 29 1.1 Prescription Example 6 30 - - 2 28 2.2 Comparative Example 1 30 - - 2 28 2.2

As can be seen from the skin irritation test results of Table 14, the cosmetic formulation containing the white bran extract was significantly lower than the cosmetic formulation does not contain the white bran extract. Therefore, it was confirmed that the cosmetic formulations containing the extract of Baekchulchae of the present invention can be effectively used in acne treatments by solving the skin irritation problem of conventional acne treatments.

As described in detail above, the present invention provides a composition for inhibiting 5α-reductase activity, sebum secretion cosmetic composition and acne relieving cosmetic composition containing baekchulchae extract as an active ingredient. The composition of the present invention effectively inhibits the activity of 5α-reductase, an enzyme that induces sebum secretion, has an excellent effect of inhibiting excessive secretion of sebum and improving acne, and thus may be usefully used for the treatment of acne.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that the specific technology is merely a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (9)

A composition for inhibiting 5α-reductase activity, which contains Chelidonium majus extract as an active ingredient. The method of claim 1, wherein the extract of the baekryeokchae composition for inhibiting 5α-reductase activity, characterized in that to suppress the amount of dihydrotestosterone production. Sebum secretion cosmetic composition containing white bran extract as an active ingredient. Acne relieving cosmetic composition containing Baekgulchae extract as an active ingredient. The method according to claim 1 to 4, wherein the extract of the white buckwheat extract is a solvent selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3 butylene glycol A composition, characterized in that it is extracted using. 6. The composition according to claim 5, wherein the white bran extract is extracted using ethyl alcohol as a solvent. 7. The composition of claim 6, wherein the ethyl alcohol is 70% ethyl alcohol. 5. The composition of claim 1, wherein the white bran extract is contained in an amount of 0.00001-20% by weight based on the total weight of the composition. The cosmetic composition according to claim 3 or 4, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and Cosmetic composition, characterized in that it has a formulation selected from the group consisting of a spray.