KR20060104807A - A method for producing extract of germinated brown rice and cosmetic composition comprising the extract - Google Patents

Abstract

The present invention relates to a method for preparing germinated brown rice extract and a cosmetic composition containing the same, by supplying brown rice with water at 30-45 ° C. in a thermostat, germinating seeds for 24 to 48 hours and rapidly growing the seeds to 2-5 mm length. Freeze drying and grinding; Putting the ground germinated brown rice into a supercritical extraction tank, injecting extracted carbon dioxide under a pressure of 71 kg / m 2 or more, and dissolving the extract using butylene glycol or squalane as a cosolvent; And it provides a method for producing a germinated brown rice extract comprising the step of eluting the extract under reduced pressure at normal pressure.

Description

A method for producing extract of germinated brown rice and Cosmetic composition comprising the extract}

The present invention relates to a method for producing a germinated brown rice extract and a cosmetic composition containing the germinated brown rice extract, and more particularly, to germinated brown rice which can maintain the activity of the active ingredients contained in germinated brown rice and obtain an optimal purified extract. It relates to a method for producing an extract and a cosmetic composition containing the same.

Since ancient times, rice contains many nutrients and has been used for many purposes such as nutrition and beauty. Rice that has been dried and threshed and then stripped of rice husk is called brown rice. Rice bran is rich in fats, proteins, essential fatty acids, essential amino acids, vitamins, dietary fiber and minerals. Vitamins in rice bran also contain vitamin B, such as myoinositol, choline, and niacin, and tocopherol, an antioxidant that prevents cell aging and damage. Minerals include phosphorus, potassium, magnesium and calcium. Rice bran also contains proteins known to lower blood cholesterol, cytosterol and dietary fiber. It is also known to have a sunscreen effect and action. Rice bran, which accounts for almost all of the nutrients in brown rice, contains not only essential amino acids, tocopherols (vitamin E), vitamins B1, B2, and B6 but also calcium, magnesium, potassium, and iron. Vitamin E improves blood circulation in peripheral blood vessels, transports oxygen and nutrients to cells, exports waste and carbon dioxide, and inhibits the increase of lipid peroxide, which prevents the oxidation of fat in the body and weakens cellular movement.

Conventional methods of extracting grains include extracting by alcoholic fermentation, extracting oil from rice by supercritical carbon dioxide, and extracting by standing in methanol, ethanol, isopropyl alcohol or acid or alkaline solution. When extracted in this manner, skin irritation may be caused by the use of an organic solvent, or active substances contained in rice may be easily lost due to heat, acid, and alkali. Japanese Patent Publication No. 91-76908 discloses a method of extracting oil and fat contained in rice at 20 to 100 ° C. by softening a rice that has not been coated with supercritical carbon dioxide with water or other humectants. . However, this method has a drawback of the loss of the water-soluble vitamins and proteins contained in the rice by extracting only the useful components, and the extraction of the active ingredients that are weak at heat because of the extraction at high temperatures. In addition, Japanese Patent Application Laid-Open No. 92-352716 discloses rice extracts that are effective in bath solvents, cosmetics, and medicines by extracting rice with water or organic solvents and then fermenting alcohol. Organic solvents and fermented alcohols, which are water, have disadvantages that are not suitable as cosmetics used for application to the skin. In addition, EP-542398 discloses pulverized powdered rice, which is boiled using water as a solvent, and then compressed to squeeze or extract the rice extract to prepare a transparent rice extract, and adding amylase enzyme to the extract. Disclosed is a rice extract developed for the use of food, cosmetics, health cosmetics effective to remove the free radicals by preventing the aggregation phenomenon. The rice extract disclosed herein has a safe side when it is put into the skin and oral cavity, but is extracted at a high temperature, so there is a big disadvantage in the loss of the active ingredient.

Korean Patent No. 0135251 discloses a method of preparing a rice extract having a whitening effect using polyhydric alcohol, lactic acid and sodium lactate, but in this case, in order to lower the pH or increase the extraction capacity depending on the content of lactic acid and sodium lactate. When the content of the polyhydric alcohol is high, there is a disadvantage that the skin irritation is induced.

The present inventors have introduced a room temperature supercritical extraction method to solve these problems.

In the extraction method of the present invention, the carbon dioxide reaches a supercritical phase at a temperature of 31 ° C. or higher and an air pressure of 71 kg / m 2 or higher, in which the optimum extract can be obtained without destroying the active ingredient. Since the carbon dioxide supercritical extraction method is performed at room temperature, it is possible to obtain the optimum purified extract while maintaining the activity of the active ingredients contained in the germinated brown rice. Therefore, when it is formulated in cosmetics it can provide an increase in cleansing power and moisturizing power, erythema soothing effect.

In order to achieve the above object, the present invention is to supply water to a 30 ~ 45 ℃ thermostat of brown rice to germinate seeds for 24 to 48 hours to rapidly freeze drying and grinding if the seed length is 2 to 5mm; Putting the ground germinated brown rice into a supercritical extraction tank, injecting extracted carbon dioxide under a pressure of 71 kg / m 2 or more, and dissolving the extract using butylene glycol or squalane as a cosolvent; And it provides a method for producing a germinated brown rice extract comprising the step of eluting the extract under reduced pressure at normal pressure.

The temperature of the supercritical extraction tank can be maintained at 30 ~ 70 ℃.

The present invention also provides a cosmetic composition containing the germinated brown rice extract obtained according to the method of the present invention.

The germinated brown rice extract using the butylene glycol as a co-solvent may be diluted in purified water and used in the form of germinated brown rice water, and the germinated brown rice extract using squalane as a co-solvent may be used as an oil germinating brown rice oil. Germinated brown rice extract of the present invention can further enhance the effect by using alone or in combination with the germinated brown rice powder obtained by grinding the frozen germinated brown rice.

The content of germinated brown rice water is 0.1 to 60% by weight, the germinated brown rice oil is 0.1 to 30.0% by weight, the germinated brown rice powder may be used in each or a mixture of 0.1 to 5.0% by weight.

Germinated brown rice is sprouted by preserving the seeds of brown rice and supplying appropriate temperature, oxygen, and moisture, and is freeze-dried after sprouting 2-5mm after initiation or growth. Seeds, also called embryos, contain about 60% of the nutrients in brown rice, but are lost almost entirely in homes through milling and washing.

Germinated brown rice is beneficial for skin that is distinguished from normal reproduction during grain germination, and various enzymes are activated, and vitamins B1, B2, B6, B12, pantothenic acid, nicotinic acid, and folic acid are increased as grains sprout. It is reported. In addition, the substances related to the immune system of the human body react with each other by the action of the enzyme produced during germination to generate an immune increasing substance such as arabinoxysilane. Dietary fiber in regular and germinated brown rice is different. Germinated brown rice has high adsorption capacity of activated carbon or sponge and has high adsorption capacity of harmful substances. It also absorbs nutrients and helps them to be absorbed slowly, allowing skin cells to fully utilize the nutrients. In addition, gamma-orizanol, which is a rice eye component, is low-molecularized by enzymes during germination, and thus has a good affinity with skin lipids to diffuse into the skin and form a sufficient film. The result is a good ultraviolet protective effect. In particular, it absorbs a wavelength of 300 to 310 nm, and produces erythema soothing effect.

Germinated brown rice is extracted by room temperature carbon dioxide supercritical extraction method, and it is in the form of germinated brown rice oil, germinated brown rice water, or germinated brown rice powder, or a mixture of them, such as softener, lotion, essence, nourishing cream, foam cleansing, cleansing lotion, Moisturizing power, erythema soothing effect and cleansing power were confirmed by adding it to skin cleansers such as cleansing cream, cleansing water, cleansing oil and cleansing gel.

Preparation Example of Germinated Brown Rice Extract

Moisturize the brown rice with 30 ~ 45 ℃ thermostat and germinate seeds for 24 ~ 48 hours. If the seeds grow to 2 ~ 5mm in length, they are rapidly frozen in -20 ℃ freezer and dried. It is crushed and placed in a supercritical extraction tank, and sealed and injected with carbon dioxide under pressure of 300 kg / m 2 through a pump. The internal temperature is kept at 40 ° C. Butyl glycol (99% Kyowa Hakko Kogyo Co., Ltd) was used as a cosolvent to raise the pressure to 300 kg / m 2, and then added to an extraction tank to dissolve the extract, and then depressurize and elute the contents to atmospheric pressure. The eluted extract was centrifuged to remove contaminants and diluted with purified water to produce germinated brown water. Germinated brown rice oil is extracted in the same manner as above by using squalane (Kishimoto Co. LTD) as a co-solvent.

In the present invention, by producing a cosmetic composition with the extracted germinated brown rice water (germinated brown rice water), germinated brown rice oil (germinated brown rice oil) and the like to reduce the skin irritation, excellent facial cleansing effect, erythema soothing effect and skin moisturizing power, May include creams, lotions, gels, water, foams, and the like. In addition, by using a germinated brown rice powder (germinated brown rice powder) can further enhance the effect of the present invention.

Hereinafter, the present invention will be described in more detail with reference to examples and comparative examples, but examples according to the present invention may be modified in various forms, and the scope of the present invention is not limited to these examples.

Table 1 was prepared by the conventional method for the softening longevity containing germinated brown rice extract of the present invention and the softening longevity not containing it.

Ingredient Name weight(%) Example 1 Comparative Example 1 Germinated Brown Rice 30 - 1,3-butylene glycol 5.0 5.0 Monolauric acid polyoxyethylene sorbitan 1.3 1.3 Polyoxyethylene lauryl ether 0.7 0.7 ethanol 15.0 15.0 Spices 0.2 0.2 antiseptic Quantity Quantity Purified water Balance Balance

Hereinafter, a lotion containing germinated brown rice extract and a lotion not containing the same were prepared in a conventional manner using the ingredients shown in Table 2.

Ingredient Name weight(%) Example 2 Comparative Example 2 Stearic acid 0.5 0.5 ethanol 10.0 10.0 vaseline 1.0 1.0 Squalane 2.5 2.5 Liquid paraffin 6.0 6.0 Monooleic acid polyoxyethylene sorbitan 1.5 1.5 1,3-butylene glycol 8.0 8.0 Sodium Ethylenediaminetetraacetic Acid 0.05 0.05 Triethanolamine 0.8 0.8 Spices 0.2 0.2 antiseptic Quantity Quantity Antioxidant Quantity Quantity Germinated Brown Rice 30 - Germinated Brown Rice Powder 0.5 - Germinated brown rice 5 - Purified water Balance Balance

Essences containing germinated brown rice extract and essences not containing the same were prepared in the usual manner with the components shown in Table 3 below.

Ingredient Name weight(%) Example 3 Comparative Example 3 glycerin 5.0 5.0 1,3-butylene glycol 5.0 5.0 Sesquioleate sorbitan 0.4 0.4 Polylauric acid polyoxyethylene sorbitan 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 ethanol 2.0 2.0 Purified water Balance Balance Vaseline 5.0 5.0 Cetanol 0.2 0.2 Required 0.5 0.5 Germinated Brown Rice 30 - Germinated brown rice 5 - Germinated Brown Rice Powder One - Purified water Balance Balance

To the ingredients of Table 4, a cream containing the germinated brown rice extract and a cream not containing the same were prepared in a conventional manner.

Ingredient Name weight(%) Example 4 Comparative Example 4 Stearic acid 0.5 0.5 ethanol 10.0 10.0 vaseline 1.0 1.0 Squalane 2.5 2.5 Liquid paraffin 6.0 6.0 Monooleic acid polyoxyethylene sorbitan 1.5 1.5 1,3-butylene glycol 8.0 8.0 Sodium Ethylenediaminetetraacetic Acid 0.05 0.05 Triethanolamine 0.8 0.8 Spices 0.2 0.2 antiseptic Quantity Quantity Antioxidant Quantity Quantity Germinated Brown Rice 30 - Germinated brown rice 5 - Germinated Brown Rice Powder 0.1 - Purified water Balance Balance

To the ingredients of Table 5 to prepare a W / O type of cleaning cream containing germinated brown rice extract and W / O type of cleaning cream not containing it in a conventional manner.

Ingredient Name weight(%) Example 5 Comparative Example 5 Mineral oil 10.0 20.0 Isopropyl Palmitate 5.0 10.0 Beads wax 2.0 2.0 Ceresin Wax 1.0 1.0 antiseptic Quantity Quantity Spices Quantity Quantity Tocopheryl Acetate 0.2 0.2 Germinated Brown Rice 30 - Germinated brown rice 5 - Polyglyceryl 4-isostearate 2.0 2.0 Solbitan Sesquioleit 1.0 1.0 Purified water Balance Balance Butylene glycol 5.0 5.0 Sodium chloride 1.0 1.0 Germinated Brown Rice Powder 1.0 1.0

To the ingredients of Table 6, O / W type cleansing cream containing germinated brown rice extract and 0 / W type cleansing cream not containing the same were prepared.

Ingredient Name weight(%) Example 6 Comparative Example 6 Mineral oil 15.0 25.0 Isopropyl Palmitate 10.0 10.0 Cetyl alcohol 0.22 0.22 Stearic acid 3.0 3.0 Vaseline 3.0 3.0 Tocopheryl Acetate 0.2 0.2 antiseptic Quantity Quantity Spices Quantity Quantity Germinated brown rice oil 5.0 - Germinated Brown Rice 22 - Polysorbate 60 2.5 2.5 Sorbitan monostearate 1.0 1.0 Glyceryl Monostearate 0.5 0.5 Purified water Balance Balance Butylene glycol 5.0 5.0 Sodium Ethylenediaminetetraacetic Acid 0.05 0.05 Germinated Brown Rice Powder 1.0 -

To the ingredients of Table 7 was prepared according to a conventional method O / W type cleansing lotion containing germinated brown rice extract and lotion not containing it.

Ingredient Name weight(%) Example 7 Comparative Example 7 Mineral oil 10 10 Isopropyl Palmitate 5.0 5.0 Cetyl alcohol 0.5 0.5 Stearic acid 1.0 1.0 Vaseline 1.0 1.0 Tocopheryl Acetate 0.2 0.2 antiseptic Quantity Quantity Spices Quantity Quantity Germinated brown rice oil 5.0 - Germinated Brown Rice 22 - Polysorbate 60 2.0 2.0 Sorbitan monostearate 1.0 1.0 Glyceryl Monostearate 0.5 0.5 Purified water Balance Balance Butylene glycol 5.0 5.0 Sodium Ethylenediaminetetraacetic Acid 0.05 0.05 Germinated Brown Rice Powder 0.5 -

To the ingredients shown in Table 8, a cleansing gel (gel) containing a germinated brown rice extract and a cleansing gel not containing the same by a conventional method was prepared.

Ingredient Name weight(%) Example 8 Comparative Example 8 Cetyloctanoate 30.0 35.0 Trioctanoine 5.0 10.0 Polyoxyethylene (25) octyldodecyl ether 15.0 15.0 antiseptic Quantity Quantity Spices Quantity Quantity Germinated brown rice oil 5.0 - Germinated Brown Rice 22 - Purified water Balance Balance Polyoxyethylene (10) Methyl Glucoside 5.0 5.0 glycerin 5.0 5.0

The cleaning foam containing the germinated brown rice extract and the cleaning foam not containing the same were prepared according to a conventional method using the components shown in Table 9 below.

Ingredient Name weight(%) Example 9 Comparative Example 9 Lauric acid 4.0 4.0 Myristic acid 6.0 8.0 Stearic acid 10.0 12.0 antiseptic Quantity Quantity Spices Quantity Quantity Germinated Brown Rice 20.0 - Germinated brown rice oil 2.0 - Germinated Brown Rice Powder 1.0 - PEG-60 Glycerylisostearate 5.0 5.0 Purified water Balance Balance glycerin 5.0 5.0 PEG-4000 2.0 2.0 Potassium hydroxide 4.0 4.0

To the ingredients of Table 10, according to a conventional method, a clean water containing germinated brown rice extract and a clean water not containing the same were prepared.

Ingredient Name weight(%) Example 10 Example 11 Purified water Balance Balance ethanol 7.0 7.0 Propylene glycol 5.0 5.0 Butylene glycol 3.0 3.0 Polysorbate 20 2.0 2.0 antiseptic Quantity Quantity Pigment Quantity Quantity Spices Quantity Quantity Germinated Brown Rice 30.0 -

To the ingredients of Table 11 to prepare a cleaning water containing a germinated brown rice extract and a cleaning water not containing it in a conventional manner.

Ingredient Name weight(%) Example 11 Comparative Example 11 Mineral oil 60.0 60.0 Cetyloctanoate 20.0 20.0 Polyethylene Glycol-20 Glyceryl 15.0 15.0 Triisostearate 3 3 antiseptic Quantity Quantity Spices Quantity Quantity Butylene glycol 2.0 2.0 Purified water Balance Balance Germinated brown rice 2.0 -

Experimental Example 1 Inhibitory Effect of Germinated Brown Rice Extract on Erythema Formation

In order to examine the erythema soothing effect of the germinated brown rice extract extracted according to the present invention by the ultraviolet rays, Examples 1 to 4 and Comparative Examples 1 to 4 were evaluated. The experimental method measures the effect of cell regeneration by the change in chromaticity according to the color discoloration of the protein. Skin color is measured with a chromaticity changer (Mimolta CR300) after treatment with ultraviolet 300-310nm wavelength for 2 hours inside the arm. Measurements are expressed in individual typologic angles (ITA). Higher ITA values indicate clean skin. The calculated value is based on the following equation.

SN (%) = (Di-Do) / (Dn-Do) x 100

Where SN: skin normalization

Di: intermediate measurement

Do: maximum chromaticity, initial value (0% normalization)

Dn: final value (100% normalized)

Table 12 shows the result of measuring 20 subjects.

sample Skin Normalization Cycle (day) Example Comparative example One 12.3 ± 2.1 14.2 ± 2.8 2 12.5 ± 3.5 14.0 ± 2.1 3 11.8 ± 4.5 14.3 ± 3.5 4 12.6 ± 2.8 13.8 ± 2.8

Experimental Example 2: Skin Moisturizing Effect of Germinated Brown Rice Extract

In order to determine the moisturizing power of the formulation containing the germinated brown rice extract extracted according to the present invention, the moisture retention capacity of Examples 1 to 4 and Comparative Examples 1 to 4 was prepared. Moisture retention capacity was measured by using a skin moisture meter (Corneometer: Courage + Khazaka, GmbH, Germany) to measure the electrical conductivity of the surface of the skin, compared to the embodiment in a constant temperature and humidity room maintained at 25 ℃, 40% relative humidity The amount of water loss was measured 30 minutes and 1 hour after face washing using the sample of the example. Table 13 shows the results of three measurements of 20 subjects.

Example Comparative example One 2 3 4 One 2 3 4 Average value before application 76 76 75 76 75 76 74 73 After application 30 minutes 96 92 98 97 85 88 85 84 medium 1 hours 91 95 94 92 78 80 78 77

Experimental Example 3: Skin irritation of germinated brown rice extract

In order to examine the skin irritation of the formulation containing the germinated brown rice extract extracted according to the present invention, the samples of Examples 1 to 4 and Comparative Examples 1 to 4 prepared above were patched with 20 adults for 48 hours and then 30 minutes to After 1 hour, the skin condition was visually inspected for changes, and the result was firstly determined, and then removed and read again after 48 hours to make the second determination. The results are shown in Table 14 below.

sample Face Arm Example Comparative example Example Comparative example One G G E E 2 E E E E 3 E E E E 4 E E E E

Very good (Excellent: E), Good (G), Medium (M), Bad (Bad: B)

Experimental Example 4

The following test was conducted to determine the makeup removal power of the facial cleansing containing germinated brown rice extract.

Make-up removal test was applied to the test lipstick on the inside of the lower arm of the left arm in a width of 3cm x 2cm, repeated cleansing using the preparations made in Examples 5-11 and Comparative Examples 5-11, respectively, and then skin color strength before cleansing. The removal ability of the makeup was evaluated by measuring the skin color strength after washing and comparing the color strength before and after cleansing. Color intensity was measured using a color difference meter CR-200 (CHROMA METER CR-200). Makeup removal ability of the face-wash cosmetics was compared by the removal index according to the following formula.

Table 15 shows the comparison results of the makeup removal power of Examples 5 to 11 and Comparative Examples 5 to 11.

Color intensity of bare skin Color intensity after applying lipstick Color intensity after cleansing Removal index (%) Example 5 4.78 45.30 6.99 95.1 Example 6 4.64 45.12 7.02 94.7 Example 7 5.62 46.75 8.53 93.8 Example 8 5.85 46.39 10.32 90.3 Example 9 5.07 45.56 7.48 94.7 Example 10 4.78 45.30 6.98 95.1 Example 11 5.63 46.76 8.54 93.8 Comparative Example 5 4.92 45.21 9.04 93.2 Comparative Example 6 4.87 45.03 11.06 90.9 Comparative Example 7 5.31 45.92 13.58 81.9 Comparative Example 8 5.19 45.87 20.87 65.8 Comparative Example 9 5.56 46.42 14.70 80.3 Comparative Example 10 4.93 45.22 9.05 93.3 Comparative Example 11 5.32 45.93 13.59 81.9

As can be seen from the results of Table 10, Examples 5 to 11 can be seen that the makeup removal ability is superior to Comparative Examples 5 to 11.

As described above, the present invention relates to a method for preparing the germinated brown rice extract and a cosmetic containing the germinated brown rice extract, and when combined with the germinated brown rice extract extracted in the present invention, the erythema soothing effect, increased moisturizing power, and stimulating relaxation function are shown. It was. In addition, when the germinated brown rice extract of the present invention is contained in the cleansing cosmetics, the makeup removal power is excellent.

Claims (8)

Supplying brown rice with water at a temperature of 30 to 45 ° C., germinating seeds for 24 to 48 hours, and rapidly freezing drying and pulverizing the seeds with a length of 2 to 5 mm; Putting the ground germinated brown rice into a supercritical extraction tank, injecting extracted carbon dioxide under a pressure of 71 kg / m 2 or more, and dissolving the extract using butylene glycol or squalane as a cosolvent; And A method for producing a germinated brown rice extract comprising eluting the extract under reduced pressure at normal pressure. The method of claim 1, wherein the temperature of the supercritical extraction tank is maintained at 30 ~ 70 ℃. A cosmetic composition comprising the germinated brown rice extract obtained by the method according to claim 1. The cosmetic composition according to claim 4, wherein the germinated brown rice extract is used in the form of germinated brown rice water or germinated brown rice oil or a combination thereof. The cosmetic composition according to claim 4, wherein the content of germinated brown rice water is 0.1 to 60.0% by weight. The cosmetic composition according to claim 4, wherein the content of germinated brown rice oil is 0.1 to 30.0% by weight. The cosmetic composition according to claim 3, further comprising a germinated brown rice powder obtained by grinding the frozen germinated brown rice. The cosmetic composition according to claim 7, wherein the content of germinated brown rice powder is 0.1 to 5.0% by weight.