KR100563442B1 - Organic farming natural extract having moisture-protection and stimulus-relief activities, and cosmetic composition comprising the same - Google Patents

Abstract

The present invention relates to an organic natural extract having a skin moisturizing and irritating effect, specifically a baby cosmetic composition containing a mixed extract obtained from organic wheat, apples, bananas.

According to the present invention, it is possible to provide a cosmetic composition for infants with excellent safety that helps moisturizing function of the skin and at the same time relieve irritation given to the skin.

Description

Organic farming natural extract having moisture-protection and stimulus-relief activities, and cosmetic composition comprising the same}

Figure 1 shows the results of the moisturizing power measurement using the desiccator for the Examples and Comparative Examples in a bar graph.

Figure 2 shows the results of cell viability test (MTT ASSAY) as a bar graph as a stimulation relaxation test in artificial dermis for the Examples and Comparative Examples.

Figure 3 shows the results of the IL-1α production measurement experiment as a bar graph in the artificial dermis for the Examples and Comparative Examples.

4 is a bar graph showing the results of the measurement of moisturizing force using Corneometer CM 825 for the Examples and Comparative Examples.

The present invention relates to a cosmetic composition derived from a natural extract, and more particularly to a cosmetic composition for infants having a skin moisturizing and stimulating effect, including extracts from organic wheat, organic apples, organic bananas.

The skin is the largest organ in the body and is always in contact with the outside, acting as a protective shield to protect the living body from various irritation or dry environments. It also plays a very important role in protecting the body from physical abrasions, preventing the loss of water in the body, protecting it from harmful ultraviolet rays from the sun, and regulating the body's body temperature. For these skin, moisture acts to transport various bioactive substances and keeps the skin soft and moist. Among them, the moisture level of the stratum corneum is important, the stratum corneum of the epidermis contains 10 to 20% of moisture, and not only prevents the evaporation of moisture, but also prevents the penetration of excess moisture from the outside. For example, polyester fibers contain about 0.4% moisture and cotton 8.5% moisture, while the stratum corneum contains a large amount of moisture and has the unique power to prevent moisture from flying away. This is similar to a structure in which a plastic wrap that does not pass water is wrapped in a sponge drenched in water. That is, it can be considered that a thin oil film is wrapped on the surface of the stratum corneum to prevent evaporation of moisture. This thin oil film is called a natural protective film. Natural protective film is a thin film that covers the surface of the skin and consists of sebum from sebaceous glands and sweat from cold glands. Natural protective film is a water-in-oil (W / O) -type emulsified state in which water is partially mixed with oil in a normal state, but when sweating, the sweat evaporates quickly so that the body temperature is kept constant.油, O / W) emulsified state. After all, the sebaceous membrane, which greatly affects the appearance of the skin surface, is undergoing a reversible transition between water-in-oil and oil-in-water. In other words, the natural protective film reversibly converts the water-in-oil type or the oil-in-water type according to the condition of the skin and contributes to moisture control.

Natural Moisturizing Factor (NMF) and Stratum Corneum Lipid also help to maintain the moisture balance in the horny layer. These are called natural moisturizing facotr (NMF), collectively known as water-soluble ingredients that are present in the stratum corneum to hold and retain moisture. The softness of the stratum corneum is due to the action of NMF, thus maintaining proper moisture. When the skin's moisturizing power is lowered, it becomes less flexible and dries, and the resistance is lowered, which makes it sensitive to irritation and becomes susceptible to damage.

Baby skin also protects the body from germs and releases sweat and heat to regulate body temperature. In addition, the lungs, internal organs, kidneys to help function of the body to remove the waste, but there is a difference from adults. This is why many babies have sweat bands because their skin, as well as other major organs such as sweat glands, are still immature and lack the body's ability to control their temperature and control their temperature. . In addition, it easily loses moisture and becomes dry and thus susceptible to damage. In this way, the baby's skin is the same as the adult but its appearance is not enough in terms of function, and it is only about 3 years old to become the thickness of adult skin, so the baby's water retention is a basic requirement for maintaining healthy skin. It is important. In addition, soft baby skin is thin and soft, sensitive to external irritation and bacterial infections, and penetrating ability is much faster than adults, so sweat or bacteria remaining on the skin penetrates into the skin again, which causes the skin to crush. Skin problems may occur. In other words, baby's skin is soft, delicate and sensitive, so skin troubles occur even on small external stimuli, and it is easy to react sensitively to small stimuli to raw materials used in skin care products. It is also important to select and use raw materials that can alleviate the risk. In addition, in order to prevent skin problems, it is most important to keep the baby's skin moisturized, relieve irritation, and keep it clean by using a gentle baby skin care product. (See BAYER KOREA, BAYER HEALTHCARE)

In this way, the baby's skin can become dry when it is not moisturized, and this dry skin responds to even small irritation, which can develop into atopic dermatitis. Therefore, the weaker and more sensitive skin, the more moisturizing and stimulating function is needed. I will.

Thus, the present inventors have diligently researched to find a cosmetic that meets the above necessities. As a result, the mixed extract from the organic natural ingredients wheat, apple, and banana are excellent in the safety on the skin, and are effective and effective in moisturizing and stimulating relaxation. This invention was completed.

Therefore, the main object of the present invention is to provide a natural extract excellent in safety and excellent in moisturizing and stimulating relaxation ability.

Another object of the present invention is to provide an organic cosmetics for infants containing the extract to help moisturizing function of the skin to moisturize and at the same time relieve the irritation given to the skin.

In the present specification, wheat ( Triticum aestivum LINNAEUS ) is a herbaceous plant 1-2 years old, about 1m in height, and 2-3 generations grow together and are flat and long nodes. Flowers bloom in May, and fruit ripens in June-July. Fruit and fruit are broad ellipsoid, brown, and fall well in spirit. Plant as spring crops everywhere. Berries contain 53 ~ 70% starch, 11% protein, 2 ~ 7% sugar (sucrose, glucose, etc.), 2 ~ 10% of textulin, 1.6% of oil, cytososterol, lecithin, and allantoin. The wheat contains tocophenol, which prevents skin aging and makes the skin elastic. It is also used as a cold sweat, bone fever, and digestive medicine. (Resource Plant Ⅴ in Korea, Ji Eun Lee: Tae Jung Kim, Page: 49)

In the present specification, apple (Malus paumila Miller var.) Is a naturalized plant native to southeastern Europe and western Asia and commonly planted in orchards throughout the country. Fruits ripen in August-September. Fruits are spherical in shape, diameter is 3 ~ 10cm, both ends are concave, and skin is red on yellow background. Apple's main ingredient is sugar, which is absorbed mostly by fructose and glucose. More than 90% of malic acid is present in organic acids and includes citric acid, tartaric acid, pectin, amino acids and the like. These acids remove the fatigue substances in our body and help to make our skin hydrated and healthy. Apples contain a lot of vitamin C, which prevents the melanin from depositing on your skin, making it soft and smooth. It is also used as a medicine for duodenal ulcers and for anemia. (Resource Plant II in Korea, Ji Eun Lee: Tae Jung Kim, Page: 183)

In the present specification, the banana (Musa basjoo SIEBOLD) is a perennial herb belonging to the genus Pachoaceae, the height is about 5m, flowers in June-September and the fruit matures in October. Native to Ceylon and Malaysia, it is grown in southern Korea and Jeju Island. Bananas are rich in sugar and contain starch, calcium, carotene and pectin, a dietary fiber. Bananas are rich in vitamin A, smoothing out rough textures and carbohydrates and sugars moisturize your skin. Bananas are good for sensitive, dry and aging skin. In addition, in oriental medicine, bananas reduce heat and moisturize the intestines, which is used when constipation occurs due to thirst or dry intestines caused by heat. (Resource Plant Ⅴ in Korea, Ji Eun Lee: Tae Jung Kim, Page: 255 )

In the present specification, organic refers to cultivated with only natural organic fertilizers without using pesticides and chemical fertilizers, these organic products do not contain harmful substances such as pesticides can be used more safely as food and cosmetic raw materials.

In order to achieve the object of the present invention, the present invention provides an extract having a skin moisturizing and irritation-reducing effect obtained by pulverizing or cutting wheat, apple and banana and then extracted with an extraction solvent.

Preferably, in the extract of the present invention, and, apple and banana are characterized in that it is organically grown. In this case, as well as moisturizing and stimulating effect due to various components of the natural material, it can be used more safely as a raw material of the cosmetic composition for infants.

Preferably, in the extract of the present invention, the extraction solvent is characterized in that the solvent alone or in combination of two or more selected from the group consisting of water, ethanol, butylene glycol, propylene glycol. In the present invention, the extract was prepared for each of the extracting solvents and tested for its effect.

Preferably, in the extract of the present invention, the extraction solvent is characterized in that it is obtained by adding 5 to 30 times the volume of milled or shredded wheat, apple and banana. If the extraction solvent is less than the volume, the sample is not extracted properly, and if the extraction solvent is more than the volume, the concentration of the extract becomes thin.

Preferably, the extract of the present invention is characterized in that obtained by the extraction method by adding the extraction solvent and extracted by heating for 0.5 to 20 hours at 37 ~ 95 ℃ or immersed for 1 to 15 days at 0 ~ 37 ℃. If the extraction temperature and time is lower than the range is not extracted properly, if higher than the range may lead to denaturation of the sample.

In order to achieve another object of the present invention, the present invention provides a cosmetic composition, characterized in that containing 1 to 20% by weight based on the total weight of the cosmetic composition. If the content of the extract is lower than the above range, it is difficult to expect a substantial effect of the present invention, and if the content is higher than the above range, it is difficult to maintain the properties of the cosmetic composition and affect the stability of the product.

Preferably, in the cosmetic composition of the present invention, the cosmetic is characterized in that it is for infants only. The cosmetic of the present invention is because the organic natural extract as a main component because it is excellent in safety necessary for infants only.

Preferably, in the cosmetic composition of the present invention, the cosmetic is characterized in that it has a formulation selected from lotion, nutrition cream, body cleanser, shampoo and the like. In the present invention, various formulations containing the extract were prepared in the formulation example.

The present invention is described in detail as follows. Extracted raw materials are organic wheat, organic apples, and organic bananas from 20 to 70% of organic wheat, 1 to 30% of organic apples, and 1 to 20% of organic bananas. Grind wheat, and chop apples and bananas. As the extraction solvent, one or more solvents selected from the group consisting of distilled water, ethanol, butylene glycol, and propylene glycol are mixed and used by adding a volume of 5 to 30 times. Extraction method is a method of extracting by heating at 37 ~ 95 ℃, 0.5 ~ 20 hours in the state of preventing the evaporation of the solvent is equipped with a cooling condenser, or by extracting the active ingredient by deposition for 1 to 15 days at 0 ~ 37 ℃ Can be. The plant mixture extract thus extracted may be used by filtration or concentrated under reduced pressure and then powdered using a distillation apparatus equipped with a cooling condenser to recover the evaporated solvent. Preferably, the extract is added to the cosmetic in an amount of 0.5 to 20%, preferably 0.01 to 10% as an extract containing a certain solid content.

Hereinafter, the present invention will be described in more detail with reference to Examples. Since these examples are only for illustrating the present invention, the scope of the present invention is not to be construed as being limited by these examples.

Examples 1-7. Warming extract manufacturer

Grind, chop, and mix organic raw materials 600g, 200g apples and 200g bananas to make 1kg of mixed raw materials, add 5kg of each extraction solvent, and extract the mixture at 45 ℃ for 3 hours in an extractor equipped with a cooling condenser. After filtration and centrifugation, final filtration was performed using a 0.45 μm filter. The results are shown in Table 1. In addition, extracts were prepared in the same manner as in Examples 1 to 7 using only 500 g of apples and 500 g of bananas, except for wheat, among organic raw materials (Comparative Examples 1 to 7).

Extraction solvent Solid content (%) Yield (kg) Example 1 Distilled water 1.76 2.20 Example 2 50% hydrous ethyl alcohol 1.69 2.37 Example 3 30% hydrous ethyl alcohol 1.57 2.26 Example 4 50% water butylene glycol 1.71 2.25 Example 5 30% water butylene glycol 1.67 2.35 Example 6  50% water propylene glycol 1.64 2.44 Example 7 30% water propylene glycol 1.58 2.47

Examples 8 to 14 Room temperature extraction

Grind, chop, and mix organic raw materials 600g, 200g apples and 200g bananas to make 1kg of mixed raw materials, add 5kg of each extraction solvent, mature at 4 ℃ for 5 days, filter with 400 mesh filter cloth, and centrifuge Prepared by final filtration with 0.45 μm filter. The results are shown in Table 2 below. In addition, extracts were prepared in the same manner as in Examples 8 to 14 using only 600 g of wheat and 400 g of bananas excluding apples from organic raw materials (Comparative Examples 8 to 14).

Extraction solvent Solid content (%) Yield (kg) Example 8 Distilled water 1.01 2.11 Example 9  50% hydrous ethyl alcohol 1.16 2.02 Example 10 30% hydrous ethyl alcohol 1.85 2.06 Example 11 50% water butylene glycol 1.11 2.14 Example 12 30% water butylene glycol 1.08 2.08 Example 13 50% water propylene glycol 1.14 2.15 Example 14 30% water propylene glycol 1.09 2.12

Experimental Example 1. Measurement of moisturizing power using desiccator

The moisturizing effect of the extract was measured using the difference in the rate of water evaporation inhibition in the liquid as follows, and each extraction solvent was used as a control. In addition, solvents containing no water were excluded from the experiment.

Experiment method

After 5 ml of the organic mixed extract was titrated into a cup having a constant surface area, the sample was left for 24 hours in a desiccator adjusted to a temperature of 35 ° C. using completely dried CaCl ₂ as an absorbent, and each sample was weighed 5 times after 24 hours. Moisturizing power was measured using the average value. The results are shown in Table 3.

Example Moisturizing power (%) Moisturizing Growth Rate Comparative example Moisturizing power (%) Moisturizing Growth Rate Experiment Control Experiment Control Example 1 43.1 26.8 1.61 Comparative Example 1 41.2 26.8 1.58 Example 2 31.2 27.3 1.14 Comparative Example 2 30.1 27.3 1.10 Example 3 32.3 26.7 1.21 Comparative Example 3 31.2 26.7 1.17 Example 4 48.7 42.6 1.14 Comparative Example 4 44.4 42.6 1.04 Example 5 45.6 39.6 1.15 Comparative Example 5 42.5 39.6 1.07 Example 6 46.3 43.3 1.06 Comparative Example 6 44.8 43.3 1.03 Example 7 48.9 43.0 1.13 Comparative Example 7 45.5 43.0 1.06 Example 8 40.2 31.1 1.29 Comparative Example 8 38.2 31.1 1.23 Example 8 29.5 21.4 1.38 Comparative Example 8 27.7 21.4 1.29 Example 10 27.5 23.4 1.37 Comparative Example 10 26.3 23.4 1.12 Example 11 48.7 42.6 1.14 Comparative Example 11 45.4 42.6 1.07 Example 12 44.2 40.1 1.12 Comparative Example 12 42.1 40.1 1.05 Example 13 45.7 41.3 1.11 Comparative Example 13 44.3 41.3 1.07 Example 14 47.4 42.6 1.11 Comparative Example 14 45.6 42.6 1.07

Calculation method

   ① Moisturizing power (%) = (1- △ S / S) X 100

       S: Weight of initial sample, △ S: Weight of initial sample-Weight of final sample

   ② Moisturizing capacity increase rate (times) = experimental moisturizing power / control moisturizing power

Experimental Example 2. Stimulation relaxation test in artificial dermis: cell survival test (MTT ASSAY)

MTT assay is a method widely used for cell proliferation and toxicity by measuring the number of living cells. The living cells are water-soluble and yellow salts of MTT (3- [4,5-dimethylthiazole-2-yl] -2,5 diphenyltetrazolium bromide is reduced to a water-insoluble blue formazan derivative by succinate dehydrogenase (also called succinate dehydrogenase or mitochondrial dehydrogenase). The resulting formazan derivative is dissolved by adding a solubilizer (usually dimethylsulfoxide) and then measured for absorbance.

Experiment method

A 10 mm diameter mixture of 1 × 105 cells / ml of a type I collagen matrix, a 5-fold concentrated DMEM (DUBELCCO'S MODIFIED EAGLE MEDIUM, BRL, USA), a buffer solution and a human fibroblasts cell line (ATCC, CRL-2310) Prepare artificial dermis by inserting 200ul of fibroblasts into collagen solution. After culturing for one week, the culture medium was removed, and 1.5 ml of the new medium was placed outside the tissue culture container, and 10 μl of each test substance was applied. SLS (sodium laurly sulfate, sigma, USA) was applied, and the organic extract mixture was applied by concentration. After application, it was placed in a 37%, 5% CO2 incubator and left for 24 hours. After discarding the medium, the artificial dermis was washed with PBS and analyzed by MTT. 100 μl of MTT solution was added per well, and after 4 hours, MTT was removed, and 100 μl of DMSO was added per well, and then absorbed at 570 nm after incubation at 37 ° C. overnight. At this time, the experiment was performed in the same manner without adding the extraction mixture as a control group was compared with the cell survival rate from the difference in relative absorbance. The results are shown in Table 4.

Example  Cell survival rate (%) Comparative example  Cell survival rate (%) Example 1 113 Comparative Example 1 109 Example 2 110 Comparative Example 2 107 Example 3 100 Comparative Example 3 100 Example 4 108 Comparative Example 4 106 Example 5 103 Comparative Example 5 102 Example 6 104 Comparative Example 6 103 Example 7 109 Comparative Example 7 107 Example 8 110 Comparative Example 8 109 Example 9 107 Comparative Example 9 106  Example 10 102  Comparative Example 10 101  Example 11 101  Comparative Example 11 100  Example 12 105  Comparative Example 12 104  Example 13 104  Comparative Example 13 103  Example 14 103  Comparative Example 14 102

Calculation method

  Cell survival rate (%) = At / Ac X 100

       At: absorbance of test group, Ac: absorbance of control group

Experimental Example 3. Stimulation relief experiment in artificial dermis: IL-1α production measurement experiment

IL-1α is an interinflammatory cytokine that acts as a proinflammatory mediator, and osteoblasts, multinuclear cells, macrophages, keratinocytes, hepatocytes, fibroblasts, etc. This produces a temporary increase. In addition, IL-1α acts not only as a derivative of epithelial regeneration of wounds but also promotes the production of arachidonic acid lipoxygenase metabolites such as leukotriene B4, 5-, 12-, and 15HETE, which promotes leukocyte movement as a regulator of allergic and inflammatory responses. It is known. This measured the amount of IL-1α production.

Experiment method

10 x diameter cells were prepared by mixing a type I collagen matrix, a 5-fold concentrated DMEM (DUBELCCO'S MODIFIED EAGLE MEDIUM, BRL, USA), a buffer solution and a human fibroblasts cell line (ATCC, CRL-2310) at 1x105 cells / ml. Vacuum dermals were prepared by inserting 200 ul of fibroblasts into the collagen solution. After culturing for one week, the culture medium was removed, and 1.5 ml of the new medium was placed outside the tissue culture container, and 10 μl of each test substance was applied. SLS (sodium laurly sulfate, sigma, USA) 0.1% was applied as an irritant and 3% organic extract mixture was applied. After application, it is placed in a 37%, 5% CO2 incubator and left for 24 hours. After taking the medium, the medium taken was measured for IL-1α production. IL-1α was measured at 450nm using an human IL-1αassay kit (ENDOGEN, USA) by an enzyme-linked immunosorbent assay (ELISA). The results are shown in Table 5.

Example  IL-1α production amount (pg / ml) Comparative example IL-1α production amount (pg / ml) Example 1 89 Comparative Example 1 91 Example 2 95 Comparative Example 2 97 Example 3 101 Comparative Example 3 102 Example 4 123 Comparative Example 4 125 Example 5 119 Comparative Example 5 121 Example 6 96 Comparative Example 6 97 Example 7 113 Comparative Example 7 114 Example 8 93 Comparative Example 8 95 Example 9 105 Comparative Example 9 106 Example 10 116 Comparative Example 10 117 Example 11 101 Comparative Example 11 103 Example 12 109 Comparative Example 12 111 Example 13 115 Comparative Example 13 117 Example 14 117 Comparative Example 14 118

Below, Although a lotion, a cream, a body cleanser, and a shampoo are illustrated as a prescription example of the present invention, the cosmetic composition of the present invention The formulation is Therefore Restricted Is no.

Prescription Example 1.

Prescription examples of lotions in cosmetics containing organic natural extracts obtained from a mixture of organic wheat, apples and bananas are shown in Table 6 below. The organic natural extract here is that of Example 1.

number Raw material Formulation Example 1-1 Formulation Example 1-2 Comparative Formulation Example 1 One   Organic Plant Blend Extract 5.0 10.0 - 2 glycerin 3.0 3.0 3.0 3 Butylene Glycol 2.0 2.0 2.0 4 Propylene glycol 2.0 2.0 2.0 5 Polyoxyethylene (60) Cured Castor Oil 1.00 1.00 1.0 6 ethanol 10.0 10.0 10.0 7 Triethanolamine 0.1 0.1 0.1 8 antiseptic a very small amount a very small amount a very small amount 9 Pigment a very small amount a very small amount a very small amount 10 Spices a very small amount a very small amount a very small amount 11 Purified water Remaining amount Remaining amount Remaining amount

  <Manufacturing method>

Formulation Example 1-1,2: Add 2, 3, 4, 8 to 11 in order to dissolve it, and dissolve by heating 6 times to 60 ° C. do. Finally, add 1, 6, 7, 9, 10, and stir sufficiently to mature.

Comparative Formulation Example 1 No. 2, 3, 4, and 8 were added sequentially in order to dissolve by stirring. Then, No. 6 was heated and dissolved at about 60 ° C. Finally, add 6, 7, 9, 10, and stir sufficiently before aging.

Prescription Example 2.

Prescription examples of creams in cosmetics containing organic natural extracts obtained from a mixture of organic wheat, apples and bananas are shown in Table 7 below. The organic natural extract here is that of Example 1.

number Raw material Formulation Example 2-1 Formulation Example 2-2 Comparative Formulation Example 2 One   Organic Plant Blend Extract 5.0 10.0 - 2 Cytostolol 1.70 1.70 1.70 3 Polyglyceryl 2-oleate 1.50 1.50 1.50 4 Ceramide 0.7 0.7 0.7 5 Ceteares-4 1.2 1.2 1.2 6 cholesterol 1.5 1.5 1.5 7 Dicetylphosphate 0.4 0.4 0.4 8 Concentrated glycerin 5.0 5.0 5.0 9 Sunflower oil 10.0 10.0 10.0 10 Carboxy Vinyl Polymer 0.2 0.2 0.2 11 Xanthan Gum 0.3 0.3 0.3 12 antiseptic a very small amount a very small amount a very small amount 13 Spices a very small amount a very small amount a very small amount 14 Purified water Remaining amount Remaining amount Remaining amount

<Manufacturing method>

Formulation Example 2-1,2: Raw materials 2,3,4,5 and 6 were homogenized at a constant temperature to be referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipid and the raw materials 1,2,7,8 and 14 are mixed and homogenized at a constant temperature to pass through a microfluidizer, and then the raw material 9 is gradually added and homogenized at a constant temperature. 10, 11, 12, 13 is added to disperse to stabilize and mature.

Comparative Formulation Example 2 The raw materials 2,3,4,5 and 6 were homogenized at a constant temperature to be referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipid and the raw materials 7,8 and 14 are mixed and homogenized at a constant temperature, and then the raw material 9 is gradually added and homogenized at a constant temperature. 10, 11, 12, 13 is added to disperse to stabilize and mature.

Prescription Example 3

Prescription examples of body cleanser among cosmetics containing organic natural extracts obtained from a mixture of organic wheat, apples and bananas are shown in Table 8 below. The organic natural extract here is that of Example 1.

number Raw material Formulation Example 3-1 Formulation Example 3-2 Comparative formulation example One   Organic Plant Blend Extract 5.0 10.0 - 2 Cytostolol 4.0 4.0 4.0 3 Polyglyceryl 2-oleate 3.0 3.0 3.0 4 Ceramide 0.7 0.7 0.7 5 Ceteares-4 2.0 2.0 2.0 6 cholesterol 3.0 3.0 3.0 7 Dicetylphosphate 0.4 0.4 0.4 8 Concentrated glycerin 5.0 5.0 5.0 9 Sunflower oil 22.0 22.0 22.0 10 Carboxy Vinyl Polymer 0.5 0.5 0.5 11 Triethanolamine 0.5 0.5 0.5 12 antiseptic a very small amount a very small amount a very small amount 13 Spices a very small amount a very small amount a very small amount 14 Purified water Remaining amount Remaining amount Remaining amount

<Manufacturing method>

Formulation Example 3-1,2: Raw materials 2,3,4,5 and 6 were homogenized at a constant temperature to be referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipid and the raw materials 1, 7, 8 and 14 are mixed and homogenized at a constant temperature, and then the raw material 9 is gradually added and homogenized at a constant temperature. 10, 11, 12, 13 is added to disperse to stabilize and mature.

Comparative Formulation Example 3 The raw materials 3,4,5,6 and 7 are homogenized at a constant temperature to be referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipid and the raw materials 7,8 and 14 are mixed and homogenized at a constant temperature, and then the raw material 9 is gradually added and homogenized at a constant temperature. 10, 11, 12, 13 is added to disperse to stabilize and mature.

Prescription Example 4.

The formulation example of the shampoo in the cosmetic containing the organic natural extract obtained from the mixture of organic wheat, apple, banana is shown in Table 9. The organic natural extract here is that of Example 1.

number Raw material Formulation Example 4-1 Formulation Example 4-2 Comparative formulation example One   Organic Plant Blend Extract 5.0 10.0 - 2 Beeswax 10.0 10.0 10.0 3 Polysorbate 60 1.5 1.5 1.5 4 Sorbitan sesquioleate 0.8 0.8 0.8 5 Liquid paraffin 40.0 40.0 40.0 6 Squalane 5.0 5.0 5.0 7 Caprylic / Capric Triglycerides 4.0 4.0 4.0 8 Carboxy Vinyl Polymer 0.2 0.2 0.2 9 glycerin 5.0 5.0 5.0 10 Butylene glycol 3.0 3.0 3.0 11 Propylene glycol 3.0 3.0 3.0 12 Triethanolamine 0.2 0.2 0.2 13 antiseptic a very small amount a very small amount a very small amount 14 Pigment a very small amount a very small amount a very small amount 15 Spices a very small amount a very small amount a very small amount 16 Purified water Remaining amount Remaining amount Remaining amount

<Manufacturing method>

Formulation Example 4-1,2: After mixing 8,9,10,11,13,16 and stirring to 80 ~ 85 ° C. and putting into the manufacturing part, the emulsifier is activated and 2,3,4,5,6, 7,12 is heated to between 80 and 85 ° C., and then emulsified. After emulsification, cool down to 50 ℃ while stirring using a stirrer, add 14 times, cool to 45 ℃, add 15 times, and add 1 once when cooled to 35 ℃. It is cooled to 25 ° C. and aged.

Comparative Formulation 4: A mixture of 8, 9, 10, 11, 13, and 16 was heated to 80 ° C. to 85 ° C., and then introduced into the manufacturing unit to act as an emulsifier. 2, 3, 4, 5, 6, 7, 12 It is heated to between 80 ~ 85 ℃ to emulsify. After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and then, No. 14 was added, cooled to 45 ° C., 15 times, and cooled to 35 ° C. before aging.

Experimental Example 4. Determination of Moisturizing Power Using Corneometer CM 825

The moisturizing effect of the extract was measured using Corneometer CM 825 by Courage + Khazaka Co., Ltd., and each extraction solvent was used as a control.

Experiment method

Ten women in their 20s for each sample were divided into 2 x 2 cm2 upper arms and 8 sections, washed several times with water, and then removed with no irritation. After 15 minutes, the sample is applied evenly by dropping 10 µl into each of the two sections. After 2 hours, 5 measurements are taken per compartment using a Corneometer. The experiment was conducted in a constant temperature and humidity room with a room temperature of 21 ± 2 ℃ and a relative humidity of 50 ± 5%. The measured values were used by averaging the values obtained by measuring five times, and are shown in Table 10 using the average value of five subjects.

Calculation method

   ① Moisture (Corneometer Value) = S-△ S

      S: Corneometer Value,

          △ S: Corneometer Value after 2 hours of sample application

   ② increased moisturizing power = experimental moisturizing power-control moisturizing power

Example Moisturizing Increased moisture Comparative example Moisturizing Increased moisture Experiment Control Experiment Control Example 1 41.57 23.63 17.94 Comparative Example 1 40.37 23.63 16.74 Example 2 13.7 5.4 8.3 Comparative Example 2 12.6 5.4 7.2 Example 3 10.4 2.3 8.1 Comparative Example 3 9.6 2.3 7.3 Example 4 46.13 35.58 10.55 Comparative Example 4 45.12 35.58 9.54 Example 5 45.2 35.6 9.6 Comparative Example 5 44.2 35.6 8.6 Example 6 50.66 41.90 8.76 Comparative Example 6 49.46 41.90 7.56 Example 7 52.3 42.7 9.6 Comparative Example 7 50.1 42.7 7.4 Example 8 37.7 21.9 15.8 Comparative Example 8 36.5 21.9 14.6 Example 9 6.36 0.9 5.46 Comparative Example 9 6.05 0.9 5.15 Example 10 8.68 2.0 6.68 Comparative Example 10 7.45 2.0 5.45 Example 11 46.02 39.58 6.44 Comparative Example 11 44.02 39.58 4.44 Example 12 47.39 38.92 8.47 Comparative Example 12 45.37 38.92 7.45 Example 13 52.30 43.43 8.87 Comparative Example 13 50.25 43.43 6.82 Example 14 55.62 45.90 10.72 Comparative Example 14 53.51 45.90 8.61

Experimental Example 5. Skin primary irritation test (closed patch test)

For the extract, the effect of stimulating the stimulation was measured by the inhibition of the stimulation caused by sodium lauryl sulfate using a closed patch test.

Experiment method

Twenty subjects with dry and sensitive skin are wiped dry with 70% ethanol after wiping the test site (inside arm). A test solution was prepared in 5% of the sample and 0.08% of sodium lauryl sulfate in purified water, and 20ul of the test solution was added dropwise to the Finn chamber. Place a paper disc on each finn chamber and fix the finn chamber on the test site. After patching for 24 hours, the test site is marked with a marking pen and judgment is made after 4 hours. Judgment was classified according to the following criteria, and the results are shown in Table 11.

- Criteria - ; No erythema or unusual symptoms

     +-; Slightly redder than the surroundings

     +; Significantly redder than the surroundings

    ++; Severe redness and swelling than the surroundings

sample Number of test subjects Judgment result Stimulus ++ + +- - Sodium Lauryl Sulfate0.08% 20 One 5 12 2 0.75 Purified water 20 0 0 0 20 0.00 Example 1 5.0% Extract + Sodium Lauryl Sulfate 0.08% 20 0 0 One 19 0.05 Example 2 5.0% Extract + Sodium Lauryl Sulfate 0.08% 20 0 0 2 18 0.10 Example 3 5.0% Extract + Sodium Lauryl Sulfate 0.08% 20 0 One 5 14 0.25 Example 4 5.0% Extract + Sodium Lauryl Sulfate 0.08% 20 0 One 3 14 0.15 Example 5 Extract 5.0% + Sodium Lauryl Sulfate 0.08% 20 0 One 5 14 0.25 Example 6 5.0% Extract + Sodium Lauryl Sulfate 0.08% 20 0 0 3 17 0.15 Example 7 Extract 5.0% + Sodium Lauryl Sulfate 0.08% 20 0 0 6 14 0.30 Example 8 extract 5.0% + sodium lauryl sulfate 0.08% 20 0 0 0 20 0.00 Example 9 5.0% Extract + Sodium Lauryl Sulfate 0.08% 20 0 0 3 17 0.15 Example 10 extract 5.0% + sodium lauryl sulfate 0.08% 20 0 0 2 18 0.10 Example 11 extract 5.0% + sodium lauryl sulfate 0.08% 20 0 One 6 13 0.30 Example 12 Extract 5.0% + Sodium Lauryl Sulfate 0.08% 20 0 0 6 14 0.30 Example 13 5.0% Extract + Sodium Lauryl Sulfate 0.08% 20 0 0 7 13 0.35 Example 14 Extract 5.0% + Sodium Lauryl Sulfate 0.08% 20 0 0 3 17 0.15

Experimental Example 6. Skin Moisturizing Improvement Test

In order to confirm the effect of improving the skin's moisturizing effect on the application of the lotions and creams of Formulations 1 and 2, to the subjects with dry skin, the lotion and cream of the formulation example was applied to the left side of the face once a day for 7 days. After applying the lotion and cream of the comparative formulation example, the results of the questionnaire survey on the improvement of skin moisturizing power are shown in Table 12 below.

Applicator Respondents Very good Good usually Inadequate Formulation Example 1-1 3 7 4 One Formulation Example 1-2 4 6 5 - Comparative Formulation Example 1 2 5 6 2 Formulation Example 2-1 5 6 3 One Formulation Example 2-2 6 5 4 - Comparative Formulation Example 2 2 4 6 3

As described above, according to the present invention, as shown in the results of the experimental examples, it is possible to provide a cosmetic composition for infants with excellent safety, which helps to moisturize the skin, and at the same time, alleviates the irritation given to the skin.

Claims (8)

Extract which has skin moisturizing and irritation-relieving effect obtained by pulverizing or cutting wheat, apples and bananas and extracting them with an extraction solvent. The extract of claim 1, wherein the wheat, apple, and banana are organically grown. The extract as claimed in claim 1, wherein the extraction solvent is water, ethanol, butylene glycol, propylene glycol alone or a mixture of two or more solvents selected from the group consisting of. The extract according to claim 3, wherein the extraction solvent is obtained by adding 5 to 30 times the volume of ground or shredded wheat, apple and banana. The extract according to claim 4, wherein the extract is obtained by adding 0.5 to 20 hours at 37 to 95 DEG C and then extracting it by depositing at 0 to 37 DEG C for 1 to 15 days after adding the extraction solvent. A cosmetic composition comprising 1 to 20% by weight of the extract according to any one of claims 1 to 5, based on the total weight of the cosmetic composition. The cosmetic composition according to claim 6, wherein the cosmetic composition is for infants only. 7. A cosmetic composition according to claim 6, wherein the cosmetic composition has a formulation selected from lotion, nourishing cream, body cleanser, or shampoo.

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