KR20060072742A - Grafting breed method of new cultivar in pinus thunbergii using pick-up tongs - Google Patents

Abstract

The present invention relates to a silent breeding method of the golden bear brush, more specifically, the golden breeding of the golden bear brush using the forceps that can facilitate the grafting by fixing the grafting part as a forceps during the silent breeding of the golden bear brush using the grafting. It is about a method.

The present invention is an asexual breeding method, in particular in the asexual breeding method of the golden bear using grafting, by using a forceps to fix the area where the reception and the large tree graft golden bear brush that can be easily grafted even those who are not familiar with the grafting To provide a method of silent breeding.

In the silent breeding method of the golden bear brush of the present invention, in the silent breeding method of the yellowish golden bear brush,

Collecting the sap before the sap flows and storing it at 3-4 ° C,

Wedge-shaped processing of the receiving part grafted with the large tree,

Grafting the reception to the tree and fixing the grafting portion of the reception and the tree with tongs.

Description

Grafting Breed Method of New cultivar in Pinus thunbergii using Pick-up tongs}             

FIG. 1: Yeongam No. 1 of a golden-colored Gomsol variant in Yeongam, Jeollanam-do, Korea, with 18 years of age, 6.0 m height, 16 cm thorax diameter, and 3.0 m width of the canopy. It is a photograph.

Figure 2a to 2d is a photograph showing the appearance of inducing varieties by grafting on the year-old pedicle tree by taking the reception from the parameter.

Figure 3 is a photograph showing the appearance of mass growth by forceps grafting.

Figure 4 is a picture showing a new breed of Gom brush with a golden color nurtured by pliers.

The present invention relates to a silent breeding method of the golden bear brush, more specifically, the golden breeding of the golden bear brush using the forceps that can facilitate the grafting by fixing the grafting part as a forceps during the silent breeding of the golden bear brush using the grafting. It is about a method.

Gomsol ( Pinus thunbergii ) is an evergreen conifer tree, and more specifically, it is an evergreen tree of the conifer pine family. Gom brush is 20m in height, 1m in diameter, and bark is dark brown, and turtle shell cracks are formed. The leaves are dark green with two clustered, needle-shaped, 8-15cm long, 1.4-2.0mm butterfly, hard in quality.

Gomsol grows in the place affected by the sea and sea breezes of Korea. It grows to Baeknyeong-do on the west coast, to Wonsan on the east coast, and to Sangju area in Daejeon. Vertically, it is a species that grows naturally in the foothills and mountain clothes below 500m above sea level in Jeju Island.

Gomsol is one of the major species in coastal forests and coastal forests as well as the main species of the coast, and has excellent landscaping value, so it is planted as a major use for coastal, reclaimed landscaping, garden trees, and roadside trees. It is becoming a highly economical species.

Pines are known to cross well, but there are no hybrids with different leaves.

Plants may be artificially hybridized or mutants may be selected to create new varieties. Artificial hybridization, however, is never easy, and selecting a mutation is a very unlikely chance that one of the 1 million seedlings will develop. This mutation is also a recessive factor, most of which is suppressed in expression and adaptable to the environment. It is weak and survival rate is very low.

In order to solve this problem, researches on the preservation of new varieties using the asexual breeding method are in progress.

As a method of asexual breeding, grafting, cutting, and harvesting are applied, and recently, tissue culture has been attempted. Grafting among the asexual breeding methods maintains the genetic characteristics of the parameters as they are, so even if there is a difference in growth and flowering loss according to the management method after planting, the inherent superiority can be preserved. (Jay-Allemandand & Cornu 1986).

In the grafting test according to whether or not the grafting part is heated, the grafting rate is high at the temperature of the grafting part at 27 ℃ and the air humidity is 80%. Especially, it is reported that the warm fusion pipe method is good. It has been reported that the graft retention rate is 60-80% when the temperature is maintained (Piskornik & Hajdrowki 1995, Siniscaldo 1997).

The success of grafting is related to the affinity between the tree and the reception, the technology of grafting, the condition of the reception and the tree, the time of grafting, and the environmental conditions such as temperature and humidity.

Xi and Ding (1993) divided the grafting process into five stages in the research on grafting fusion process and influencing factors: callus formation, initiation of callus formation, callus formation, formation layer differentiation and binding, vascular differentiation and binding. Humidity and concentration of the reception were reported to be major factors in callus formation and graft survival.

Until now, new varieties have been preserved by grafting methods. The purpose of asexual breeding by grafting is to preserve clones that can maintain the characteristics of developed varieties, foster cold and disease resistant varieties, promote early fruiting, flowering fruit, Recovery of defensive power. However, grafting has a problem that the general public is difficult to do because of the difficulty of grafting the large tree and the reception. In addition, grafting is a technical grafting using vinyl tape, unexpectedly a lot of hands go uncomfortable when grafting using vinyl tape, the general public has a problem that grafting is not good.

The present invention devised in order to solve the above-mentioned problems, in the asexual breeding method of the golden bear brush using a grafting method, in particular, grafting, it is not familiar with grafting by fixing by using a forceps to the site where the reception and the large tree is grafted It is intended to provide a silent breeding method of the golden bear brush that can be easily grafted even by people.

The present invention is to develop a breeding technology of a strong cold-resistant yellowish bear breeds by using an asexual breeding method using forceps to facilitate the breeding of golden bears of genetically superior varieties such as early deletion, securing stable heritability, quality improvement, etc. Can be.

The lush breeding method of grafting using forceps according to the present invention can reduce the labor force in the growth of the golden bear brush and to facilitate the grafting even beginners can breed the golden bear brush in large quantities.

In the silent breeding method of the golden bear brush of the present invention for achieving the above-mentioned object, in the silent breeding method of yellowish golden bear brush,

Collecting the sap before the sap flows and storing it at 3-4 ° C,

Wedge-shaped processing of the receiving part grafted with the large tree,

Grafting the reception to the tree and fixing the grafting portion of the reception and the tree with tongs.

In the present invention, the receptionist uses a golden bear brush having a golden leaf, and the present invention will be described in more detail according to the above steps.

In the present invention, the golden bear brush reception can be stored at low temperature, preferably 4 ° C. or less, more preferably 3 to 4 ° C. after the sap of the reception is collected and prepared in a size of 10 ± 2 cm.

The golden bear brush can be directly grafted by receiving the reception, but usually the temperature is low before the sap of the reception flows, so the effect of grafting can be halved. Store at low temperature until

At this time, when storing the reception at low temperature, it is good to always keep the reception area to be grafted to the tree to increase the sticking rate after the reception. For example, it may be wrapped with a wet cloth to cover the reception area to be grafted or to the soil containing moisture in the reception area to be grafted.

In the present invention, the shape of the reception when grafting the golden bear brush reception to the tree can be formed in the wedge shape of the receiving portion grafted with the tree.

In the present invention, when grafting the reception to the tree can be grafted to the tree by inserting the reception of the wedge-shaped reception in the middle of the split.

At this time, the length of the wedge shape, which is the site where the reception is in contact with the tree, should be 1 to 2.5 cm. In the present invention, the application of the various sizes of the reception and reception is applied to the length of the contact with the bar, when the size of the golden bear brush reception is 10 ± 2cm, the length of the wedge shape, which is the site of the reception in contact with the tree, is 1 to 2.5 cm When grafting is done well.

In the present invention, when grafting the golden bear brush to the tree trunk, the shape of the tree can be cut into a straight line (-) and the reception and the tree can be combined by grafting the reception into the cut tree.

After the reception is grafted to the tree, the grafting site where the reception and the tree are combined can be fixed with forceps and maintained at 20 to 30 ℃ for 40 to 60 days.

In the present invention, tongs are used to pick up or integrate and fix an object, as long as it can fix the grafting part of the log and the reception. As an example of the tongs in the present invention can be used any one selected from clothespins mainly made of wood or synthetic resin, hairpin tongs mainly used by women, and tongs to keep the documents from moving as one of the office supplies.

In the present invention, the forceps fixed to the grafting part of the reception and the log can be used to have a predetermined coupling force according to the size, strength and grafting area of the reception and the log. Since the use of these tongs can be appropriately selected by those skilled in the art, details thereof will be omitted herein.

The grafting part where the reception and the tree are combined can be fixed by using forceps and then maintained at 20-30 ° C for 40 to 60 days so that the reception and grafting can be completely grafted. In the present invention, it was found that grafting is well done when the golden bear brush reception and the log are applied for various temperatures and periods.

Golden Gomsol selected as a new individual in the present invention was found to be 18 years old in Yeongam, Jeollanam-do, and found a new individual with golden leaves on its branches. It became a parameter and received the reception from this parameter.

In the present invention, the large tree may preferably use pine tree than evergreen conifer trees such as golden bear brush used for reception.

Hereinafter, the content of the present invention will be described in detail through examples and test examples. However, these are intended to explain the present invention in more detail, and the scope of the present invention is not limited thereto.

Example 1 Individual Selection

In the present invention, the parameters of the golden bear brush reception was selected in April 1995, 18 years old, 6m tall, 16cm chest diameter, 3.0m crown width, Yeongam-gun, Jeollanam-do (see Fig. 1).

The golden bear brush parameter is yellow, and the color of the leaf is yellow, and it is fixed yellow continuously since the leaf emerges. The normal bear brush is green, while the leaf is mutated and turned yellow. The results were identical.

Example 2 Variety Fixed by Grafting

Grafting was carried out in 1997 and 2004 to fix varieties of golden bear brushes.

The receptionist was collected in February 1996 and stored in a 4 ° C cold room. The preparation of the reception was made 10 cm long. And on March 30, 40 grafts were carried out in the general grafting method with tape grafting using vinyl tape and pliers with pliers.

At this time, the step of inducing the varieties by graft pliers using the forceps when grafting from the golden bear brush parameters to grafted to 1 year old pedestrian tree is shown in Figures 2a to 2d. At this time, tongs were used for clothespins.

After the completion of each grafting, the vinyl tunnel was installed, and the grafted water grafted by each grafting method was planted inside the vinyl tunnel, and the temperature inside the vinyl tunnel was maintained at 25 to 30 ° C. Then, around 20 May, the vinyl tunnel was peeled off, and the slidability of the grafted grafts was grafted by the respective grafting methods, and the results are shown in Table 1 below.

Comparative Example 1

Sliding rate after the grafting and tapering of each gom sol in the same manner as in Example 2, except that the gom sol, which is a typical green leaf and is 18 years old, is taken as a parameter, and the reception is taken from this gom sol parameter. And the results are shown in Table 1 below.

Comparative Example 2

Sliding rate after pine grafting and nipper grafting in the same manner as in Example 2, except that pines with a general green leaf and 18 years old are used as the parameters and the reception is collected from the pine parameters. And the results are shown in Table 1 below.

Table 1. Sliding rate according to species and grafting method (%)

dropsy Grafting method Grafting Running water () Slurry rate (%) Gom brush Tape Grafting * 40 37 92.5 Grafting tongs ** 40 38 95.0 Golden goal Tape grafting 40 37 92.5 Grafting tongs 40 37 92.5 Pine tree Tape grafting 40 37 92.5 Grafting tongs 40 36 90.0

* Grafting method using tape on grafting part

** Grafting method using forceps at the graft site

<Application example>

In Example 2, the golden bear brush was multiplied in large quantities using a forceps graft, and this is shown in FIG. 3.

In addition, using a forceps grafting in Example 2 fixed a new type of golden bear brush with a golden leaf color is shown in Figure 4 the appearance.

The graft slidability of the Examples and Comparative Examples was high as 92.5% general grafting using a tape, 92.5% grafting using a forceps.

However, since the grafting using the forceps of the present invention does not require any special technique, both men and women can easily grafting, and thus, a method for mass production of new varieties can be developed. The breeding of landscaping species can be carried out easily.

As described above, although described with reference to a preferred embodiment of the present invention, those skilled in the art will be variously modified and modified within the scope of the present invention without departing from the spirit and scope of the invention described in the claims below. It will be appreciated that it can be changed.

Claims (4)

In the silent breeding method of the yellowish golden bear brush, Collecting the sap before the sap moves and storing it at 3-4 ° C, Processing the part of the reception which is grafted with the big tree into wedge shape, The method of cultivating the golden bear brush using the tongs, characterized in that it comprises the step of grafting the receptionist and the grafting part of the receptionist and the lumber with forceps. The method of claim 1, wherein the acceptance of the golden bear brush using the tongs, characterized in that the size of 10 ± 2cm is prepared. The method of claim 1, wherein the storage of the golden bear brush using tongs, characterized in that the area to be grafted with a large amount of cloth when storing the reception or wrap the area to be grafted with the large tree or the area to be grafted with the soil containing moisture How to breed. The method of claim 1, wherein the graft of golden bear brush using the tongs, characterized in that the graft to the large tree and fixed with forceps, and then maintained at 20 to 30 ℃ for 40 to 60 days.

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