KR20060071661A - Skin care cosmetic compositions containing extracts of lycopus lucidus turcz - Google Patents

Abstract

The present invention relates to a composition for external application for skin containing a taklan extract, and more particularly, the composition of the present invention contains an extract extracted from at least one of the leaves, seeds, roots, and stems of the lanthanum as an active ingredient to inhibit inflammation and skin By providing effects such as moisturizing, improving blood circulation and improving skin wrinkles, it can prevent skin aging and enhance skin elasticity.

Taxane * Anti-inflammatory * Capillary strengthening * Vascular relaxation * Blood circulation * Anti-aging

Description

Skin care cosmetic compositions containing extracts of Lycopus lucidus TURCZ}             

Figure 1 shows the value measured on the basis of CTFA guideline how much the talan extract according to the present invention to alleviate the skin irritation caused by lactic acid.

Figure 2 shows a schematic diagram of a test model for evaluating the capillary permeability inhibitory ability of the talan extract according to the present invention.

Figure 3 shows the capillary permeability inhibitory effect of the talan extract according to the present invention compared with H ₂ O ₂ .

Figure 4 shows artificially induced contraction in the aortic tissue piece of the mouse, and shows the value measured by how the talan extract inhibited the contraction of blood vessels by administering the talan extract according to the present invention.

Figures 5a and 5b shows the results of measuring the blood circulation effect of the cream containing the talan extract using a Laser Doppler Perfusion Imager (LDPI).

Figure 6 shows the results of measuring changes in blood flow before and after a week of applying the essence containing the talan extract according to the present invention on the skin.

Figure 7 shows the skin temperature measured before and after a week using an IR (Infrad) camera applied to each face of the essence containing the taxane extract according to the present invention.

The present invention relates to a composition for external application for skin containing a taklan extract, and more particularly, the composition of the present invention contains an extract extracted from at least one of the leaves, seeds, roots, and stems of the lanthanum as an active ingredient to inhibit inflammation, By providing effects such as moisturizing, improving blood circulation and improving skin wrinkles, it can prevent skin aging and enhance skin elasticity.

Skin is the body's primary protective film that protects internal organs from changes in temperature and humidity, and from stimuli from the external environment such as ultraviolet rays and pollutants. In recent years, contact dermatitis, which is rapidly increasing, is a local inflammatory reaction occurring on the skin when an external irritant is applied to the skin, and clinical symptoms include irritation such as itching, tingling, burning, erythema and edema ( Inflammatory stimuli such as edema), histologically, edema is found in the dermis and monocytes are observed in the epidermis and dermis. In addition, contact dermatitis may be roughly classified into irritating contact dermatitis, allergic contact dermatitis, sensory irritation, phototoxic dermatitis, and the like according to clinical symptoms and mechanism of expression.

More specifically, irritant contact dermatitis secretes cytokines having various physiological activities by keratinocytes stimulated by a stimulating agent and acts on surrounding cells to release inflammatory mediators and cytokines. It is a non-immune reaction that induces vasodilation, vascular permeability, and migration of leukocytes, causing inflammation of erythema and edema at the site of stimulation. Allergic contact dermatitis is an immune response that is specific to chemicals, which makes it difficult to detect and has a relatively severe clinical response, sometimes leading to a systemic response. Sensory stimulation does not show clinical symptoms such as erythema or swelling and subjective symptoms such as itching, tingling and burning. Phototoxic stimuli are the photostimulatory molecules that react with light to produce a series of physiochemical reactions. The symptoms include burning, itching, erythema and edema.

However, their distinction is difficult and there are many conflicts. A common process for a substance to cause skin irritation is that the substance penetrates the skin, interacts with epidermal or dermal cells to activate the immune system, resulting in an inflammatory reaction. In general, many skin-related products contain substances that can cause irritation or inflammation, and more than 10% of people who use personal care products complain of side effects to the skin. The survey showed that more than 50% thought they were sensitive skin. In addition, the recent cosmetics tend to focus on functional cosmetics that can have a substantial effect on the skin, so it is expected that the side effects of the skin due to the use of products such as cosmetics will increase gradually. For example, lactic acid, glycolic acid, salicylic acid and retinoids are included in cosmetics to promote skin cell regeneration or anti-wrinkle effects, but most of these cosmetic products Causes irritation

Therefore, various methods have been attempted to alleviate such skin irritation, and for example, a method of reducing skin irritation has been attempted by removing a substance causing skin irritation. However, in the above method, the functional raw material itself is often used as an irritant, and more than 5,500 kinds of chemicals such as fragrances, preservatives, and bases are used, which may cause various side effects. Because of this, the research on the method of removing the substance causing skin irritation is limited.

Also, non-cosmetic drugs used for the purpose of alleviating irritation and inflammation are nonsteroidal flufenamic acid, ibuprofen, benzidamine, indomethacin, and indomethacin. Prednisolone, dexamethasone, etc. are steroidal systems, but most of them have problems in terms of safety on skin and stability when formulating skin compositions (Kim Chang-jong, Pathology, pp. 61). -69, 1988). Therefore, researches on substances having anti-inflammatory and stimulating effects while having fewer side effects to the human body are being conducted to compensate for these disadvantages.                         

On the other hand, among the causes of various skin aging recently, a study results that the skin aging caused by the sun is the main cause of the reduction of capillaries. A comparison of facial skin exposed to UV rays with hip skin that did not reveal differences in capillary number. In the hip skin that is not exposed to the sun, the size of the blood vessels decreases by more than 30% after the 70s, compared to those in their 20s and 30s. In addition to the smaller size, the number of blood vessels decreased by 43% in the 70s compared to the 20s and 30s. In particular, the areas where the capillaries were reduced were concentrated in the upper part of the dermis, just below the epidermis, where the destruction of the skin was severe, suggesting a close relationship with skin aging. In addition, the fact that the skin exposed to the sun for a long time decreases the size and number of capillaries, which reduces the ability to transport various nutrients and oxygen to the skin, and causes the skin to be wrinkled because the substances that give elasticity to the skin are not regenerated. Confirmed.

Capillaries in the skin are small blood vessels (6-10 m) that are exchanged directly and are composed of very weak and thin endothelial cells surrounded by the basement membrane without smooth muscle. Capillaries have a very large surface area and relatively high permeability, allowing the exchange of solutions, electrolytes, and large molecular weight materials. If microcirculation is not performed smoothly, edema may occur due to stagnation of blood and stagnation of lymph, and may affect nutrient supply and oxygen supply into tissues. Treatment of these edemas is achieved through the control of physical factors involved in fluid movement, for example, diuretics to reduce blood volume, reduce venous pressure, or gravity. Lift your feet high so that you won't be affected, or wear clothing or bandages around your body.

The promotion of blood circulation in the skin should be directed to preventing the permeability of the capillary wall rather than the promotion of the lymphatic circulation. This is due to the secretion of histamine and bradykinin resulting from inflammation, This is because there will be more cases of increased edema. Therefore, the method of promoting blood circulation in the skin is to relax the blood vessels to suppress the permeability of capillaries or to lower the venous pressure, thereby ultimately preventing aging of the skin.

Therefore, the present inventors conducted a study to find a new substance that can suppress skin aging by improving skin inflammation and promoting blood circulation without causing skin irritation, and as a result, it is possible to apply the extract of medicinal herb medicinal herbs as a composition for external application of skin. In this case, it was found that not only has an excellent inflammation inhibitory effect, but also promotes blood circulation in the capillaries of the skin and inhibits aging, thereby completing the present invention.

Accordingly, it is an object of the present invention to provide a talan extract having an excellent anti-inflammatory effect and blood circulation improvement effect.

It is also an object of the present invention to provide an external composition for skin containing the talan extract as an active ingredient.

In order to achieve the above object, the composition of the present invention is characterized in that it contains an extract extracted from at least one of the leaves, seeds, roots, stems of the lanthanum as an active ingredient.

The talan is contained as an active ingredient in the composition of the present invention dried dried outposts of Lycopus lucidus TURCZ , which is a perennial herbaceous plant, the form is flat and has a length of about 2 to 30 cm, and in one shot, it makes blood flow well for a long time. It is one of the common herbal medicines used by gynecologists to remove bleeding and prevent menstruation without damaging regularity.In particular, it is prescribed for postpartum abdominal pain and swelling, menstrual irregularities, menstrual pain, wounds, bruises, swelling, jaundice, stroke and high blood pressure. come.

The composition according to the invention is characterized in that it contains 0.001 to 50.0% by weight, preferably 1.0 to 10.0% by weight, based on the total weight of the composition of the extract extracted from the talan.

In addition, the topical skin composition of the present invention is not particularly limited in the formulation, for example, softening longevity, nourishing longevity, massage cream, nourishing cream, pack, gel or skin adhesive type cosmetics; Cosmetics having formulations such as lipstick, makeup base, foundation, etc .; Cleaning agent compositions such as shampoo, rinse, body cleanser and soap; Or transdermal formulations such as lotions, ointments, gels, creams, patches or sprays.

In addition, the talan extract may be commonly used to extract the active ingredient from the plant and may be prepared by a method well known to those skilled in the art. For example, the talan extract may be processed after extraction and extraction by the extraction methods of Reference Examples 1 to 3 below. Can be.

Reference Example 1

Samples obtained from at least one of the leaves, seeds, roots, and stems of the taxane grown for more than two years are dried and ground. Water, anhydrous or hydrous lower alcohol, acetone, ethyl acetate, or diethyl ether, are added to the taxane pulverized in an amount of 1 to 15 parts by volume based on the total dry weight of the pulverized product, followed by an extractor with a cooling condenser. It is heated for 5 to 24 hours at a temperature of 50 ~ 100 ℃ in to obtain a lanthanum extract. Subsequently, for preservation and antioxidant of the lanthanum extract, the lanthanum extract immediately after collection is passed through a filtration membrane having a pore size of 0.2 micron to be used as a nutrient for bacteria, microorganisms and microorganisms present in the lanthanum extract, After long-term preservation, polymer proteins that could be precipitated or denatured were removed, and anhydrous ethanol was mixed to at least 7% (w / w) to inhibit enzyme activity. Treat to prevent the invasion of microorganisms. Filtration of the extract may use both a vacuum filtration method using a vacuum pump and a pressure filtration method using a flow control tube peristaltic pump. In the examples and test examples in the present specification, taxane extract obtained in the manner of Reference Example 1 was used.

Reference Example 2                     

Water, anhydrous or hydrous lower alcohol, acetone, ethyl acetate or diethyl ether having the same amount as that of Reference Example 1 was added in an amount of 1 to 15 parts by volume based on the total dry weight of the ground product. Next, it is immersed for 3 to 20 days at a temperature of 4 ~ 25 ℃ to obtain a lanthanum extract. The dry residue is obtained in a yield of 0.01 g to 15.0 g per kg of solvent. Post extraction processing is the same as in Reference Example 1.

Reference Example 3

The same sample pulverized as in Reference Example 1 was added to the extract extracted with anhydrous or hydrous ethanol or methanol, and then the precipitate formed by filtration was removed, and ethyl acetate, butanol or diethyl ether was added thereto, followed by mixing well. After standing to separate the layers and again separating the upper layer, the extract is obtained from an extractor with a cooling condenser. Post extraction processing is the same as in Reference Example 1.

Reference Example 4 Antiseptic Effect Test

Immediately after collection, the lanthanum extract was passed through a filtration membrane having a pore size of 0.2 micron, and then immediately preserved, stored at room temperature and 45 ° C., and the preservative effect was observed with time. The results are shown in Table 1 below.

Preservative Treatment Content (% w / w) 1 day past 7 days past 30 days past Room temperature 45 degrees Room temperature 45 degrees Room temperature 45 degrees Imidazolidinyl urea 0.3 O O X X X X DM (in 10% EtOH solution) 0.3 O O X X X X DP (in 10% EtOH solution) 0.3 O O X X X X DM / DP (in 10% EtOH solution) 0.2 / 0.1 O O 0 X X X Anhydrous ethanol 2.0 O O O X X X Anhydrous ethanol 5.0 O O O O 0 X Anhydrous ethanol 7.0 O O O O O O Anhydrous ethanol 10.0 O O O O O O Anhydrous ethanol 25.0 O O O O O O

O: Preservative, X: No fungus, so fungus growth

As a result, it was confirmed that the antiseptic effect could be shown up to 30 days when anhydrous ethanol was contained 7% (w / w).

Hereinafter, although an Example and a test example are given and this invention is demonstrated more concretely, the content of this invention is not necessarily limited to these examples.

Test Example 1 Anti-inflammatory Test of Talan Extract

The anti-inflammatory effect of talan extracts was evaluated by measuring the inhibitory effect of prostaglandin production using electrocultured macrophages. First, aspirin was added to macrophages taken from the abdominal cavity of mice to a final concentration of 500 mM, thereby irreversibly inhibiting cyclooxygenase (COX) activity remaining in the cells. Then, add 100 µl of the cell suspension to each well of a 96-well plate and incubate for 2 hours in an incubator at 37 ° C. with 5% CO ₂ to attach macrophages to the vessel surface. I was. Subsequently, the attached macrophages were washed three times with PBS, and then used for anti-inflammatory test of taklan extract. Talc prepared in the manner of Reference Example 1 above was induced by the production of prostaglandin after incubation for 12 hours by adding RPMI medium containing 1% (w / v) of LPS to 5 × 10 ⁴ cells / ml of electrocultured macrophages. Extracts were diluted in purified water at 0.1% and 0.5% and treated with 100 μl, respectively, to quantify free prostaglandins using enzyme-linked immunosorbent assay (ELISA). At this time, the production inhibitory activity of the prostaglandin of the extract was set to 100% the difference between the prostaglandin produced in the LPS treated group and the untreated group and compared with the control group through the percentage of prostaglandin reduced by treating the LPS and the sample together. , And the results are shown in Table 2 below.

Inhibitory Effect of Prostaglandins Blank 100% Control group (aspirin treated group) 25.0% Thalan Extract 0.1% 36.7 Taxan Extract 0.5% 44.5

As shown in Table 2, it was found that the inhibitory effect of the production of prostaglandins by the taxlan extract was very high compared to the control treated with aspirin, and the effect was increased as the concentration of the taxlan extract was increased. .

Test Example 2 Anti-inflammatory Test of Talan Extract                     

This experiment was performed using the Carrageenan foot edema method using SD mice. One hour after administration of 40 mg / kg aqueous solution of talan extract to SD mice, 0.5 ml of 0.1% carrageenan solution was injected into the hind paw of the experimental animal to induce inflammation. After the carrageenan injection and 4 hours after the injection, the change in the paw volume of the rat was measured, and after calculating the% inhibition rate for edema using Equation 1 below, the results are shown in Table 3 below.

% Inhibition = {(1-ΔV treatment group) / ΔV control group x 100 (ΔV: change in foot volume)

Edema Inhibitory Effect of Talan Extract sample % Inhibition Control group (Aspirin 40 mg / kg) 35 ± 6 Taxane Extract 40mg / kg 43 ± 8

As shown in Table 3, it was found that the edema inhibitory effect of the taxlan extract is better than the control treated with aspirin.

Test Example 3 Local Anti-inflammatory Effect of Talan Extract

2 μg of Tetradecanoylphorbol acetate (TPA) was dissolved in 20 μl acetone and applied to the ears of ICR mice to induce edema. After 15 minutes and 6 hours of inducing edema, 40 mg of aspirin (control) and 40 mg of talan extract were diluted in 1 kg of purified water, and 0.4 mg of each was applied. 24 hours after the first TPA application, a portion of the mouse's ear was punched out, and then myeloperoxidase activity was measured to determine the ear's weight and to assess the number of neutrophils flocking to the edema site. The anti-inflammatory efficacy of each sample was measured and the results are shown in Table 4 below.

sample Weight loss inhibition rate (%) Myeloperoxidase activity inhibition rate (%) Control group (aspirin 0.4 mg / ear) 55 45 Taxane Extract 0.4mg / ear 53 48

As shown in Table 4, it was found that the anti-inflammatory effect of the talan extract compared to the control treated with aspirin.

[Examples 1-3 and Comparative Example 1]

To the components and contents shown in Table 5 below to prepare the compositions of Examples 1-3 and Comparative Example 1.

Compounding Ingredients (wt%) Example 1 Example 2 Example 3 Comparative Example 1 Purified water Remaining amount Remaining amount Remaining amount Remaining amount Lactic acid 3.0 3.0 3.0 3.0 Talan extract 0.05 0.1 0.3 - Vegetable Cured Oil 1.5 1.5 1.5 1.5 Stearic acid 0.6 0.6 0.6 0.6 Glycerol Stearate 1.0 1.0 1.0 1.0 Behenyl alcohol 2.0 2.0 2.0 2.0 Arakidilbehenyl Alcohol & Arakidilglucoside 1.0 1.0 1.0 1.0 Cetylaryl Alcohol & Cetearyl Glucoside 3.0 3.0 3.0 3.0 Liquid paraffin 11.0 11.0 11.0 11.0 Caprylic / Carric Triglyceride Glyceride 6.0 6.0 6.0 6.0 Preservative, fragrance, coloring Quantity Quantity Quantity Quantity

[Test Example 4] Determination of skin irritation effect of talan extract

In general, it was examined how the talan extract according to the present invention mitigates skin irritation caused by lactic acid, which is generally used as a cosmetic raw material and is known to stimulate skin. 20 μl of each of the compositions of Examples 1 to 3 and Comparative Example 1 were used in a circular portion having a diameter of about 1.3 cm on the skin of 15 women and 15 men (male 3 and female 12) using a spatula. The coating was rubbed 50 times. At this time, the first two times, after applying the composition, a hill top chamber without a filter paper (hill top chamber) was placed on it, and then fixed with an adhesive (adhesive) to create a closed condition. There was a chamber reaction by a hill top chamber, so when the composition was applied from 3 to 8 times, the filter paper was placed immediately after the application and fixed using an adhesive. It was patched once every 23 hours and inspected according to CTFA guidelines (1981) 1 hour after patch removal. Four days after the end of the patch, the test was further examined to see a delayed type response, which was reflected in the results. The data were compared by checking the equality of the Levin test (Levene's test) using the last test result and then post hoc test (p <0.05) through ANOVA (Duncan). The determination of the results was made according to the measurement criteria shown in Table 6 below, and the results are shown in Table 7 below and FIG. 1. The results shown in Table 7 are the sum total of the response index of the test subjects responding to the stimulus by the number of times, Figure 1 is a bar graph showing the sum of the stimulation index value for each sample.

Rating sign Criteria 0 - No visible reactions One + Mild erythema 2 ++ Strong erythema 3 +++ Strong erythema with edema 4 ++++ Intense erythema with edema & vesicle

Patch count Example 1 Example 2 Example 3 Comparative Example 1 1 time One One 0 3 Episode 2 One One One 5 3rd time 0 0 0 3 4 times 0 0 0 2 5th 0 0 0 3 6th One 0 0 4 7th 0 0 0 2 8th 0 One 0 7 4 days after end One One One 10 Sum 4 4 2 42

As shown in Table 7, in the case of the composition of Comparative Example 1 does not contain the lanthanum extract, while the irritation response to the skin by lactic acid is very high, Examples 1 to 1 containing the lanthanum extract For the composition of 3 it can be seen that the skin irritation caused by lactic acid is relatively low. In addition, it was found that the stimulation-releasing effect of the talan extract was higher skin irritation-releasing effect when used in quantitative use.

Test 5 Skin irritation test of talan extract

After applying the composition of Examples 1 to 3 once daily to the lower arm of 20 healthy male and female experimenters, a plastic cap was put on to block the outside. After 1 day, 3 days, and 7 days of application, the degree of stimulation was observed according to the determination method of Table 8, respectively, and the average value of the calculated stimuli was shown in Table 9 below.

Judgment number Skin irritation degree 0 1 2 3 4 No irritation Minimal irritation Slight irritation (erythema) Severe irritation (erythema, edema) Severe irritation (erythema, edema)

sample Skin irritation (%) 1 day later 3 days later 7 days later Example 1 Example 2 Example 3 Comparative Example 1                                              0.3 0.2 0.5 0.3                                              0.3 0.3 0.6 0.4                                              0.4 0.5 0.8 0.6                                             

As shown in Table 9, it was shown that almost all skin irritation in the compositions of Examples 1 to 3, Comparative Example 1.

Test Example 6 Determination of Hygroscopicity of Talan Extract

Talc extract and distilled water were saturated and absorbed into human dry epidermal cells, respectively, and the amount of water absorbed was measured, followed by mass change after 48 hours of drying. The amount of water absorbed per unit of skin cell mass was compared from each measured change in mass. This experiment is a general method for comparing the amount of water absorbed by epidermal cells and is used as a way to anticipate the moisturizing effect on human skin.

First, the human epidermal cells were dried to make the amount of water contained per unit mass constant, and then the weight of each epidermal cell sample and the amount of water contained therein were measured using the Kaiser method. The epidermal cells of which the water content was measured were saturated and soaked in 3 ml of diluent diluted 40 mg of talc extract in 1 kg of purified water for 24 hours, and then the epidermal cells were taken out, weighed, and partly collected by a Kaiser moisture meter. The amount of water per unit mass contained was measured. Thereafter, the resultant was dried under reduced pressure for 48 hours and weighed, and then, a part of the sample was collected and water content was measured by Kaiser method. The amount of water per unit mass contained therein was measured, and the results are shown in Table 10 below. .

Moisture supply capacity for epidermal cells (moisture mg / epidermal cell mass g) Moisture Content in Early Drying Saturated water content Moisture content after drying Taylan Extract 450 780 590 Purified water 450 740 510

As shown in Table 10, it was confirmed that the epidermal cells saturated with the thylan extract contained much more moisture than the epidermal cells saturated with the purified water, from which the excellent skin hygroscopicity was confirmed.

Test Example 7 Experiment of Capillary Permeability Inhibition of Talan Extract

Capillary permeability inhibitory ability of the taxane extract according to the present invention was measured as follows.

CPAE cultured pulmonary endothelial cell lines dispensed from the Korean Cell Line Bank were cultured in RPMI-1640 (10% fetal bovine serum) medium at 37 ° C and 5% CO ₂ , followed by 2x10 ⁵ cells. Cells were placed in a 12-well plate (Costar Transwell Clear, # 3460, tissue culture treated polyester membrane, pore size 0.4 mm) and incubated for 3-4 days until confluent. When confusion was confirmed, the medium was changed to RPMI-1640 of 4% FBS and further cultured for 3 days. All monolayers were identified using phase contrast microscopy and confirmed fusion again just before the experiment. The plate used in the test (FIG. 2) consists of an upper luminar compartment and a lower albuminal compartment, with semipermeable membranes between the compartments. Evans blue (EB, Sigma, 90 mg / ml) was used as a reagent. 0.5 ml of bovine serum albumin (BSA, Sigma) solution (40 mg / ml) (control) was added to the upper lumina portion of the plate using a Hank buffered saline solution (HBSS, pH 7.4). 1.5 ml of Hank's buffered saline solution (HBSS, pH 7.4) was added thereto. After being left for a certain time, 75 ml of the solution was taken from the lower albumin part, placed in a 96-well plate, and the absorbance was measured at 630 nm. The test substance was an EB-BSA solution in which Hanm buffered saline was added H ₂ O ₂ 1 mM and an EB-BSA solution in which talan extract (1, 10, 100 ppm) was added. And absorbance were measured in the same manner as the control. Each test material was pre-incubated 2 hours before the start of the test, and the results are shown in FIG. 3.

As shown in FIG. 3, when the talan extract was treated with 1, 10, and 100 ppm, respectively, capillary permeability was suppressed to 123.2%, 115.7%, and 110.3% as compared with the addition of 1 mM of H ₂ O ₂ . It was confirmed, which indicates that the taxane extract according to the present invention has a blood circulation promoting effect by inhibiting the permeability of capillaries.

Test Example 8 Vascular Relaxation Test of Talan Extract

Vascular relaxation of the talan extract was measured as follows. Unlike capillaries, blood vessels such as arterioles, lavage veins, small arteries, or predetermined veins can regulate blood circulation according to the contraction and relaxation ability of blood vessels.

After bleeding the mice, the thoracic aorta was removed, a vascular tissue section was placed in an organ chamber, and then connected to a force displacement transducer (Grass Co.). After loading 2g of tension and equilibrating for 60 minutes, phenylephrine 10 ^-7 M was put into the organ chamber to cause contraction. When contraction was maintained at a constant state after phenylephrine administration, talancholine extracts were administered to measure vascular relaxation ability, and the results are shown in FIG. 4.

As shown in FIG. 4, when the talan extract was treated with 100 ppm and 200 ppm, the vasoconstriction effect was restored to 43% and 71%, respectively. It has the effect of promoting blood circulation.

Test Example 9 Blood Circulation Measurement Experiment Using LDPI

Blood for the composition of the present invention containing a taxlan extract using a laser Doppler Perfusion Imager (LDPI), which is widely known as a device for measuring blood circulation in the skin and is still in use. Circulation promoting capacity was measured. LDPI is known to be a very sensitive instrument that can measure not only the velocity and volume of blood in the capillaries of the skin but also the flow in the arteries and the venous veins.

First, after washing the arms and hands using soap in a constant temperature and humidity room in which the LDPI is located, after adapting for 30 minutes, the initial value was measured in both arms using the LDPI. A total of 10 participants (5 males and 5 females) participated in the experiment, and the average blood pressure was measured by measuring the amount of blood changes at 4 sites per arm. To apply the cream of Comparative Example 2 (control) and Example 4 (containing talan extract) having the components shown in Table 11 in the same amount to the left and right arms of the subject, respectively, and after 1 hour to measure the degree of blood circulation using LDPI, The results are shown in FIGS. 5A and 5B.

ingredient Content (% by weight) Comparative Example 2 Example 4 glycerin 3.0 3.0 Butylene glycol 2.5 2.5 Propylene glycol 2.0 2.0 Squalene 5.0 5.0 Capric / Capric Triglycerides 3.3 3.3 Stearic acid 0.5 0.5 Cyclovinyl Polymer 0.3 0.3 Liquid paraffin 7.0 7.0 Beeswax 4.5 4.5 Cetostearyl alcohol 1.5 1.5 Polysorbate 60 1.5 1.5 Glycerin Monostearate 4.5 4.5 Sorbitan monosulfate 0.05 0.05 antiseptic Quantity Quantity Talc - 2.0 Purified water Remaining amount Remaining amount Sum 100.0 100.0

As shown in FIGS. 5A and 5B, when the cream of Example 4 containing the taxane extract was applied, it was found that blood circulation was promoted by determining the increase in blood flow due to the decrease of the blue portion and the increase of the red portion.

Test Example 10 Measurement of Blood Flow Improvement Effect on Face

11 women with cold hands and feet in the constant temperature and humidity room were washed with soap for 30 minutes, and then adjusted for 30 minutes using LDPI and IR (Infrad) camera, a skin temperature measuring instrument. Measured. LDPI was used to measure the initial blood flow in the lower part of the forehead of the test subject, and the initial temperature of skin temperature at the forehead, above the eyes, under the eyes, the chin and the cheek surface was measured using an IR camera. To prepare an essence (Example 5) containing the substances of the components shown in Table 12 and using the blood flow and skin temperature after using for one week compared with the initial measured value, the results are shown in Table 13, 14 and 6, 7 is shown.

Example 5 ingredient Content (% by weight) Purified water Remaining amount glycerin 8.0 Pentaerythritol tetraisostearate 5.0 Butylene Glycol 5.0 Cyclomethicone (Decamethylcyclopentasiloxane) 5.0 Hyaluronic Acid Extract 2.0 Vegetable squalane 8.0 C14-22 Alcohol / C12-20 Alkyl Glucoside 4.0 Stearic acid 2.0 Cetearyl Alcohol 1.5 Glyceryl Stearate 2.5 Talan extract 2.0 Preservative, coloring, fragrance Quantity

LDPI results 1 week before and after essence Face Before use 1 week after use medium 0.973 1.158

IR results before and after essence use (t-test, p <0.05) forehead On snow Under the eyes Cheek chin Before use 33.24 32.96 32.67 30.75 32.07 1 week after use 33.68 33.53 33.25 31.67 32.68

As shown in Tables 13 and 14 and FIGS. 6 and 7, there was a statistically significant increase in blood flow after using essence (Example 5) containing taxane extract for 1 week on the face. In addition, as shown in the results of Table 14 and Figure 7, the skin temperature of the face measurement area was all increased, and in particular, showed a significant skin temperature improvement effect on the forehead, eyes and cheeks after 1 week of essence use.

Test Example 11 Measurement of Skin Elasticity Improvement Effect of Talan Extract

The skin elasticity improving effect of the cream of Example 5 shown in Table 11 was measured. After dividing 20 healthy women over 30 years old at a temperature of 24 to 26 ° C and a humidity of 75% into two sets, and applying the cream of Comparative Example 2 and Example 5 to each group in the same amount twice daily for 12 weeks, Skin elasticity was measured using a skin elasticity measuring instrument (Cutometer SEM 575, C + K Electronic Co., Germany). The results are described as ΔR8 (R8 (left) -R8 (right)) values of Cutometer SEM 575, where R8 values represent skin viscoelasticity. Separately, at the end of the test, the subjects were asked to fill out a questionnaire, which was evaluated mechanically and subjectively. The results are shown in Tables 15 and 16 below.

Skin elasticity improvement effect of talan extract Experimental product Skin elasticity effect Comparative Example 2 0.14 Example 4 0.47

Survey results on skin elasticity improvement Applicator Respondents (Personal) Very good Good usually Inadequate Comparative Example 2 One 3 3 3 Example 4 3 6 One 0

As shown in Table 15 and Table 16, the skin elasticity of the group coated with the cream of Example 4 containing the taxlan extract was increased compared to the group coated with the cream of Comparative Example 1. At the same time, it was confirmed that the skin elasticity was improved when the cream of Example 4 was applied through a questionnaire.

Hereinafter, another example of the formulation of the present invention will be described, but the formulation of the external preparation containing the talan extract according to the present invention is not necessarily limited to these examples, and these are all moisturizing skin, improving blood circulation, alleviating skin inflammation and skin wrinkles. Have improved efficacy.

Formulation Example 1 Flexible Cosmetics

ingredient Content (% by weight) Purified Water Betaine Natto Gum Cellulose Gum Ethanol Polyoxyethylen Hardened Castor Oil Tocopherol Acetate Preservative Pigment Talcane Extract Balance 3.0 3.0 0.08 5.0 0.5 0.2 5.0 Trace 1.0

Formulation Example 2 Nutritional Cosmetics

ingredient Content (% by weight) Purified water 7-ethylhexanoate pentaerythryl distearate lipophilic monostearic acid stearate squalane cetearylglucoside hydroceticitin glycerin triethanolamine carboxyvinyl polymer preservative pigment fragrance talan extract Balance 5.0 1.0 0.8 2.0 1.5 0.5 8.0 0.2 0.2 Trace Trace Trace 3.0

Formulation Example 3 Nutrition Cream

ingredient Content (% by weight) Purified Water Beeswax Glyceryl Stearate Cetostearate Cetearyl Glucoside Hydrogenated Lecithin Sacchiethylhexanoate Squalane Liquid Paraffin Glycerin Propylene Glycol Plant Extract Antiseptic Pigment Aromatic Talan Extract Balance 1.0 2.0 2.5 1.5 0.5 5.0 5.0 8.0 8.0 4.0 5.0 Trace Trace Trace 3.0

[Formulation Example 4] Essence

ingredient Content (% by weight) Purified Water Glycerine Propylene Glycol Cellulose Gum Natto Gum Hyaluronic Acid Extract Amino Propanesulphonic Acid Carboxyvinyl Polymer Ethanol Polyoxyethylene Hardened Castor Oil Triethanolamine Preservative Flavors Balance 15.0 5.0 0.05 5.0 5.0 2.0 0.5 5.0 0.5 0.5 Trace Trace Trace 1.0

[Formulation Example 5] Lotion

ingredient Content (% by weight) Purified Water Glyceryl Stearate Cetaryl Glucoside Hydrogenated Lecithin Cethylethylhexanoate Squalane Lanolin Glycerin Carboxyvinyl Polymer Hydrolyzed Collagen Triethanolamine Preservatives Fragrances Pigment Extracts Balance 1.5 0.5 3.0 3.0 2.0 8.0 0.5 1.0 0.5 Trace Trace Microbalance 1.0

Formulation Example 6 Ointment

ingredient Content (% by weight) Purified Water Caprin / Capryl Triglyceride Liquid Paraffin Hydrogen Lecithin Octyldodeces-25 Sacchiethylhexanoate Squalane Glycerin Salicylic Acid Glycerin Sorbitol Taxane Extract Balance 10.0 10.0 6.0 9.0 10.0 1.0 1.0 15.0 10.0 1.0

Formulation Example 7 Spray

ingredient Content (% by weight) Purified Water Triethanolamine Polyvinylpyrrolidone / Vinyl Acetate Glycerin Polyacrylate Taxane Extract Remaining 0.2 3.0 5.0 0.2 1.0

As described above, the composition according to the present invention has effects such as skin moisturization, skin blood circulation improvement, skin inflammation prevention and improvement, and skin wrinkle improvement, and when the cosmetic composition of the present invention is applied to the skin, the above action Ultimately, it can inhibit skin aging and enhance skin elasticity.

Claims (7)

An external preparation composition for skin, comprising an extract of talan. The composition for applying the external of the skin according to claim 1, wherein the extract is extracted from at least one of the leaves, seeds, roots and stems of the talan. The composition for applying the external of the skin according to claim 1, wherein the talan extract is contained in an amount of 0.001 to 50.0 wt% based on the total weight of the composition. According to claim 1, wherein the composition for external skin composition, characterized in that for moisturizing the skin. According to claim 1, wherein the composition is an external composition for skin, characterized in that for improving blood circulation. According to claim 1, wherein the composition is an external composition for skin, characterized in that for preventing and improving skin inflammation. According to claim 1, wherein the composition is an external composition for skin, characterized in that for preventing and improving skin wrinkles.

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