KR20060061054A - Process for cultivating crops containing phyto-selenium with mushroom - Google Patents

Abstract

The present invention relates to a method for cultivating an organic selenium-containing composite crop, and more particularly, to inoculate mushrooms to a crop containing an inorganic selenium solution, in which a selenium is metabolized during the growth of the mushroom, the organic selenium has a pharmacological function. A method for causing organic selenium to be contained in a crop by allowing it to be converted to a form. According to the present invention it is possible to provide a crop product with a competitive advantage by cultivating high value-added functional crops.

Mushrooms, selenium

Description

Process for cultivating crops containing phyto-selenium with mushroom}

1 is a photograph showing a process of growing mushrooms by mixing inorganic selenium and rice.

2 is a photograph showing functional rice containing organic selenium grown by the method of the present invention.

Figure 3 is a photograph showing the black soybeans containing organic selenium grown by the method of the present invention.

4 is a photograph showing meso-beans containing organic selenium grown by the method of the present invention.

5 is a photograph showing a mixed composition containing organic selenium grown by the method of the present invention.

Figure 6 is a photograph showing the brown rice raw material powder containing organic selenium grown by the method of the present invention.

The present invention relates to a method of cultivating organic selenium-containing crops, and more particularly, inoculates mushrooms to a crop containing an inorganic selenium solution, in which the selenium undergoes metabolic processes of the mushrooms, and has an organic pharmacological function. A method of converting into selenium form to allow organic selenium to be contained in crops.

Mushroom has been used for edible and medicinal for about 3,000 years, and it is reported that the mushroom-derived materials are useful for the treatment or prevention of various cancers and for the prevention of cancer caused by immune enhancement. Mushrooms are divided into Mycelium and Fruiting Body. Mycelium means that spores of mushrooms germinate to form a thread. Abundance of bioactive substances and germs are germinated to form sprouts It is called a mushroom.

Situation mushroom is a rare rare perennial mushroom that grows in the alpine region, such as wild mulberry, oak, chestnut, and oak, and is known as Phellinus linteus (Perlinus linteus). . Situation mushrooms are mostly parasitic from ancient trees that have been growing for decades, and are used in traditional Chinese medicine such as `` herbaceous tree '' and `` agreement bogam '' and `` different '', `` shangmok '' and `` sinsin ''. It is recorded in the record under the name of “Year”. The shape of the situation mushroom is initially called a muddy lump, but after it is grown, it is sometimes called snowy because it is a tongue stump that is placed on a tree stump. The upper part of the tongue is shaped like mud or blackish like the skin of persimmon, and the lower part is like a yellow carpet, and the upper part is black or mud. When the mushrooms are sweet, they appear yellow or pale yellow, and they have no taste and aroma. They are mild and light, and are easy to eat. Situation mushroom fruiting body is a perennial mushroom that grows in the form of mud at first, stops growing in winter, turns yellow to mud, and turns yellow again in spring.

As the immune system of the human body is activated, it is extremely difficult to develop a disease, and thus the medical community has recently been actively researching the human immune system. When cancer develops, the "human immune mechanism" acts to prevent its growth or metastasis. As the immune mechanism, researches on mushroom polysaccharides have been actively conducted. Among them, research results have been published that polysaccharides contained in situation mushrooms are very effective in treating cancer and have excellent effects. Pharmacological studies on the pharmacological action of situational mushrooms have been known to activate immune function following chemotherapy following liver cancer surgery, including gastric cancer, esophageal cancer, duodenal cancer, colon cancer, and rectal cancer, which are cancers of the digestive system. have. According to a study by the Japanese Cancer Center, the tumor suppression rate of the situation mushroom is 96.7%, which is the strongest anticancer activity. It is also known to activate and detoxify uterine bleeding and treatment, dysmenorrhea, intestinal bleeding and intestinal function.

On the other hand, selenium is present in trace amounts in seawater and soil and is an essential mineral element in human cells. It was first discovered by Berzelius in 1817 and named selenium after Selen, the Greek mythological moon goddess, and in 1957 was identified by Dr. Klaus Schwarz as a mineral essential for the activation of human cell function. I started to receive. Phospholipids, which occupy most of the cell membranes, contain a large amount of unsaturated fatty acids. Lipid peroxides are those that are automatically oxidized by free radicals. In other words, when the cell membrane is chemically damaged by free radicals, the ability to defend the cell itself is lost, and the DNA, a genetic factor, is damaged. If the phospholipids in the cell membrane are attacked by free radicals and become lipid peroxides, and thus the cell membranes are not protected, the cells lose their natural function and proliferate abnormally and are converted to cancer cells. Healthy people neutralize themselves before free radicals damage our bodies, but when excessive free radicals occur at one time or when the body's defense capacity decreases, free radicals destroy the cells of our bodies in turn to promote aging of the human body. Attacks genes and causes mutations to cause cancer The body's defense enzyme that prevents the oxidation or destruction of cells by free radicals is glutathion-peroxidase, which is synthesized in the liver, and selenium is essential as a major component. In other words, the deficiency of selenium in the living body has a significant effect on the function of the human body, there is a risk of various adult diseases such as heart disease, joint disease, vision disease, aging of cells, deterioration of human immune function and aging and onset. The recommended daily dose of selenium is 75-200 μg. Selenium has been shown to prevent cancer, prevent metastasis, help prevent angina and myocardial infarction, and have anti-aging effects. Selenium is a mineral that is necessary to make `` prostaglandin '', a hormone that regulates blood pressure in the body, and it is effective in preventing atherosclerosis, thrombosis, myocardial infarction, and cerebral thrombosis by preventing blood vessels from dilation and stiffening. In addition, it has a role as an antioxidant to remove the fat peroxide that causes aging. In other words, if a large amount of lipid peroxide is produced in the body, it may cause adult diseases such as arteriosclerosis, liver failure, diabetes, and cataract, but selenium is a component of an enzyme called glutathione-peroxidase, which breaks down the lipid peroxide. And play an important role in the activity of this enzyme. Therefore, selenium has the effect of anti-aging.

Among the functional crops that are currently on the market, for example, rice has been shown to include nutrients such as chitosan, minerals and calcium, as well as natural substances such as gungjungjang rice, ginseng rice, mushroom rice and green tea rice. New functional rice that does not need to be washed is also emerging. In addition, other bio-rice (bioactive rice), red mushroom rice, live rice (germ) are on the market.

Therefore, there is an urgent need for the development of a system for providing agricultural products in a safe manner without adverse effects on the human body by allowing minerals to be absorbed into plants in a more efficient manner.

As a result of earnestly researching the formulation of selenium which can be directly absorbed by the consumer, the present inventors added selenium to grains or crops such as brown rice, brown rice, black rice, and cereals, which are commonly used in diet, and cultured mushrooms using them as a medium. As a result, it is possible to prevent various diseases in advance by ingesting a crop of mushrooms containing the beneficial substances of various efficacy, such as preventing various adult diseases, and organic selenium and nutritional ingredients showing various functions as described above through a daily meal Was unexpectedly found and the present invention was completed.

Accordingly, an object of the present invention is to complex-grown crops containing organic selenium and crops containing organic selenium obtained by culturing mushrooms (eg, situation mushrooms) on crops (eg, rice) to which selenium is added. To provide a method.

Another object of the present invention is to provide a method for producing the organic selenium situation mushroom rice to improve the income of growers and to greatly contribute to human health.

A further object of the present invention is to easily consume a crop containing organic selenium that is naturally biosynthesized through daily diet, supplying energy to the human body and at the same time preventing or preventing various diseases that may occur. The present invention provides a method of cultivating a crop containing a high concentration of organic selenium that can be treated.

It is another object of the present invention to provide a method for biosynthesis of organic selenium in various crops at low cost and high concentration in a short time.

It is still another object of the present invention to provide a crop containing organic selenium having a high concentration of organic selenium in a state in which a second biological treatment of crops or imported crops grown on general soil to contain organic selenium at a high concentration is performed. It is.

The present invention provides, in one aspect, a crop containing an organic selenium obtained by culturing a mushroom on a crop to which selenium is added, and a method of cultivating the crop so that the organic selenium is contained in the crop.                     

That is, the present invention is characterized by converting inorganic selenium into organic selenium through biological metabolism by mixing the grains of mushrooms and rice, etc., as a nutrient for the mushrooms, and functional rice, black medicinal beans, and soybeans by this technique. It is possible to produce various products such as organic selenium miso, organic selenium red pepper paste, organic selenium raw material, as well as grains and mixed compositions.

Hereinafter, the present invention will be described in more detail.

As used in the present specification, the term "crop" refers to any edible or medicinal crop having commercial properties that are cultivated in an agricultural or biological manner, for example, fruits, horticulture, vegetables, as well as cereals. Include all of them. Therefore, in a specific embodiment of the present invention will be described for a specific crop, but the present invention is not limited to these, for example, rice, brown rice, brown rice glutinous rice, black rice, cereals, barley, soybeans, apples, pears, strawberries It is obvious that it includes tomato, bellflower, ginseng, and the like.

The method of providing an organic selenium-containing composite crop according to the present invention is inoculated with mushrooms in the cultivation process of the already grown crops or crops, followed by culturing by adding an inorganic selenium solution thereto, or blending a solution containing selenium to the crops. After inoculating mushroom bacteria, the inorganic selenium is converted into organic selenium through the culturing process, so that it is possible to provide a consumer with a composite crop containing organic selenium (eg mushroom + rice). The addition form of the selenium may be added in the form of a powder, but it may be more preferable to add the liquid in the mushroom absorbed. In the case of providing the inorganic selenium as a solution, it is appropriate to use the inorganic selenium solution at a concentration of 100 ppm, and it is preferable to incubate by adding a final concentration of 10-50 ppm to prepare grains during the culture. As a result of the test of the present inventors, the addition of inorganic selenium at a final concentration of 10 ppm or less leads to a very low conversion rate of organic selenium, and addition of more than 50 ppm of inorganic selenium may cause problems in the conversion of organic selenium and in the growth of microorganisms. have. When the growth time is more than 100ppm, the growth period is long, and it is confirmed that the growth of the mushrooms is not grown, but only a part of it.

Mushroom bacteria used in the present invention is not particularly limited, but the situation mushroom bacteria is most preferred, and may be added together with a medium (eg, malt extract liquid medium). Crops are most suitable grains as possible, but the present invention is not limited to a specific crop. Mushroom bacteria are suitably inoculated in an amount of 5 to 10% based on the total volume. Cultivation is preferable to stationary culture rather than shaking culture.

Therefore, in the specific embodiment of the present invention,

a) adding malt to sterile water and sterile filtration to adjust the sugar content of the malt extract to brix 9 ~ 12 to prepare a malt extract liquid medium,

b) pure culture of Phellinus linteus in malt extract liquid medium at 250 ml for 7-10 days,

c) sterilizing the cleanly washed brown rice in a culture vessel;

d) adding the inorganic selenium solution to the rice in a concentration of 10 to 50 ppm based on the total volume,

e) inoculating 5-10% of the pure cultured situation mushroom in the mixed solution,                     

f) incubating the inoculum at 25 to 28 ° C. for 10 to 15 days,

g) after inoculation, if the mycelium begins to grow on the rice surface as a whole, shake the culture bottle once every 2 to 3 days to prevent rice from clumping,

h) after completion of the culture step of drying after freeze drying or drying using a 75 ℃ hot air dryer

It provides a method for producing organic selenium-containing situation mushroom rice comprising a.

Steps d) and e) inversely mix rice and mushrooms, and then may be preferably used to add an inorganic selenium.

To this end, it is preferable to first incubate Phellinus linteus in malt extract liquid medium. The malt extract liquid medium is added with 100 ~ 500 g of malt in 1 liter of purified water and left for 8 hours to 10 hours at 40 ~ 70 ° C, then boiled at 90 ~ 120 ° C for 5-10 minutes, and then filtered and brix the sugar content of the malt extract. 9-12 was used as the medium. Separately, clean 250 ~ 500 g of brown rice and clean it for 8-10 hours, sterilize for 30 minutes to 2 hours at 121 ℃ in a 1 liter plastic culture container, and cool it to room temperature. Add to Shake the selenium evenly and then inoculate 5 to 10% of pure cultured S. mushrooms and incubate at 25 to 28 ℃ for 10 to 15 days. After inoculation, if the mycelium begins to grow on the rice surface as a whole, shake the culture bottle every two to three days to prevent rice from clumping. After completion of the culture, the organic selenium-containing situation mushroom rice is prepared by lyophilization or drying using a 75 ° C. hot air dryer.

Alternatively, Phellinus linteus was incubated in malt extract liquid medium, and then 250 to 500 g of brown rice was washed and placed in a culture vessel, sterilized at 121 ° C. for 60 minutes, cooled to room temperature, and the pure cultured bacterium was cultured. Inoculate 5-10% and incubate for 10-15 days at 25-28 degreeC. After inoculation, put 10 ~ 50 ppm of inorganic selenium solution at the time when mycelium grows to the whole rice, and shake it so that it can be mixed as a whole. In addition, shake the bottle every 2 to 3 days to prevent rice from clumping. After completion of the culture, the organic selenium-containing situation mushroom rice may be prepared by lyophilization or drying using a 75 ° C. hot air dryer.

If the inorganic selenium remains unconverted to organic selenium, the microorganism will not be cultured. Since microorganisms are not higher organisms, such as mammals or humans, if they are not suitable for themselves, they do not become cultures themselves.

In general, it is possible to expect the prevention of various diseases by ingesting organic selenium through the diet, but also considering that crops grown on ordinary soils have very small amounts of organic selenium, so its effect is difficult to expect. Inventions have been made.

The price of agricultural products is falling gradually, and the import of low-quality, high-quality foreign crops is increasing. Under these circumstances, selenium-containing crops can be of sufficient value in the market for low-priced foreign produce, and even if foreign produce is imported, they can be sufficiently competitive.

According to the present invention, a technique of applying an inorganic selenium solution to a plant and converting it into organic selenium in the plant through the metabolic process of the mushroom may be applied to crops already grown, but may also be applied to crops being grown.

Accordingly, the present invention further provides a high concentration of organic selenium in a crop, which is characterized by increasing the content of organic selenium in the crop by repeatedly applying the inorganic selenium solution 1 to 20 times according to the growing season of the crop depending on the growing environment of the crop. To provide an accumulation method.

<Example>

Hereinafter, the present invention is described in more detail by the following representative examples, but the present invention is not limited in any way by these examples.

Example 1

Phellinus linteus was purely cultured in malt extract liquid medium. The malt extract liquid medium was placed in 1 L of purified water and 250 g of malt oil was left at 60 ° C. for 10 hours, then boiled at 100 ° C. for 10 minutes, and filtered to adjust the sweetness of the malt extract to brix 11 to use as a medium. 350 g of brown rice was washed clean, soaked for about 8 hours, and then sterilized at 121 ° C. for 60 minutes in a 1 L plastic culture container. It was cooled to room temperature and then an inorganic selenium solution was added to a concentration of 50 ppm. The selenium was shaken well and then inoculated with 10% inoculated purely cultured mushrooms and incubated at 25-28 ° C. for 15 days. After inoculation, when the mycelia began to grow on the rice surface as a whole, the culture bottles were shaken every three days to prevent rice from clumping. After completion of the cultivation using a frozen 75 ℃ hot air dryer was dried and screened to prepare an organic selenium-containing situation mushroom rice.

Example 2

After culturing Phellinus linteus in malt extract liquid medium, 200g of brown rice was washed and placed in a culture vessel, sterilized at 121 ℃ for 60 minutes, cooled to room temperature, and then inoculated with 5% of pure cultured bacterium 25 Incubated for 15 days at ~ 28 ℃. After inoculation, when the situation mycelium grows throughout the rice, the inorganic selenium solution was added to 10 ppm, and the mixture was shaken to mix. In addition, shaking the bottle every two days to prevent rice from clumping. After completion of the culture, the organic selenium-containing situation mushroom rice was prepared by lyophilization or drying using a 75 ° C. hot air dryer.

Example 3

In the same manner as in Examples 1 and 2, was applied to brown rice, black rice, soybeans, black weak beans, mixed grains and the like for 15 days, the results are shown in Figures 3 to 6, respectively. 3 to 6 are photographs showing black sesame beans, soybeans, mixed compositions, and brown rice raw raw material powders each containing organic selenium.

Test Example 1: Organic Selenium Content Test

In the situation mushroom rice according to Example 1, the results were analyzed by the Korea Basic Science Institute (Analyzer-ICP / MS) to determine whether the selenium contained (organic selenium content was significantly increased according to the treatment. The results are shown in Table 1 below. Selenium was not detected in the control group not treated with selenium.

Organic Selenium Content (mg / kg) Experiment 0.06 Control   Not detected

As described above, according to the present invention, by producing organic selenium-containing crops and providing biosynthesis of organic selenium at low cost and high concentration within a short time, the chemical method is eliminated, and minerals are efficiently treated through plant metabolism. By being absorbed into the plant, it is possible to provide minerals in a safe manner without adverse effects on the human body, and also to easily ingest nutrients from the crops, to exhibit the pharmacological effects represented by the organic selenium, thereby contributing to the improvement of health, and the organic selenium By making it contain, it is possible to combine the functional and palatability of a crop, and to provide a crop.

Claims (3)

A composite crop containing organic selenium obtained by culturing mushrooms on a crop to which selenium is added. The method of claim 1, wherein the rice is grown together with the situation mushroom. A method of growing an organic selenium-containing complex crop, characterized in that the inorganic selenium is mixed with the crops and used as a medium for mushrooms, or the crops and the mushrooms are mixed and the inorganic selenium is added.

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