KR20060053634A - Cosmetic composition for prevention of skin aging containing the extract of decorticated glycine max merr as active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing skin aging containing peeled black bean extract as an active ingredient, and more specifically, a composition for promoting collagen synthesis containing the peeled black bean extract as an active ingredient and skin aging containing the composition as an active ingredient. It relates to a cosmetic composition for prevention. Peeled black soybean extract of the present invention has excellent collagen biosynthesis promoting effect and antioxidant effect can be usefully used in the manufacture of cosmetics for preventing skin aging.

Peeled Black Bean Extract, Anti-Aging Skin, Collagen Production, Cosmetic Composition

Description

Cosmetic composition for preventing skin aging containing peeled black bean extract as an active ingredient {Cosmetic Composition for Prevention of Skin Aging Containing the Extract of Decorticated Glycine max Merr as Active Ingredient}

The present invention relates to a cosmetic composition for preventing skin aging containing peeled black bean extract as an active ingredient.

Skin maintains homeostasis such as humidity, temperature, and protects the body from physical and chemical stimuli from the outside. However, ageing or constant exposure to external stimuli, especially in the sun, can lead to rapid skin aging that damages the skin and degrades physiology. As the skin ages, the demand for quality of life increases due to natural phenomena or improvement of living standards that inevitably occur with age, and skin aging that has been increased due to various environmental factors. Countermeasures are urgently needed.

A common intracellular phenomenon in photoaging and natural aging is the increase in reactive oxygen species (ROS). These reactive oxygen species cause carcinogenesis, inflammation and aging ( Clinics in dermatology , 1996, 14: 227-234). The increase in reactive oxygen species with aging is attributed to the increased production of ROS and the reduction of antioxidant levels. Reactive oxygen species directly affect proteins and DNA in vivo, altering their function, but on the other hand, altering the signaling processes that underlie various metabolism, resulting in biobalance, which eventually accumulates for a long time and eventually Symptoms of aging will appear.

The most prominent cosmetic feature associated with skin aging is the generation of wrinkles. Wrinkles occur on the face of the forehead, around the eyes, between the legs, and around the mouth, or on different parts of the body, such as the neck, neck, elbow, armpits, hands, and feet. Most are noticeable around 30 years of age, and as they age, their number, depth, and range increase. The main cause of wrinkle formation is that reactive oxygen species generated in skin tissue by UV irradiation or natural aging are formed by forming crosslinks in collagen fibers and elastic fibers, which make up most of the dermal constituents of the skin, and changing their formation and degradation. ( Clinics in geriatric medicine, 2001, 17 (4): 617-630).

Therefore, it is necessary to apply a substance having an antioxidant effect as part of inhibiting the aging of the skin, and to develop a substance capable of steadily maintaining the synthesis of collagen collagen, which is a major cause of wrinkles.

Throughout this specification, numerous patent documents and articles are referred to and their citations are indicated. The disclosures of cited patent documents and articles are incorporated herein by reference in their entirety, and the level of the technical field to which the present invention belongs and the gist of the present invention are more clearly explained.

The inventors of the present invention prepared an extract for a plurality of natural products to solve the above-described needs of the art to perform a task of selecting a substance excellent in antioxidant and collagen synthesis effect, peeled black bean extract excellent collagen The present invention was completed by confirming the synthetic effect, the antioxidant effect and the wrinkle improvement effect.

Accordingly, it is an object of the present invention to provide a composition for promoting collagen production.

Another object of the present invention to provide a cosmetic composition for preventing skin aging.

Other objects and advantages of the present invention will become apparent from the following examples and claims.

According to an aspect of the present invention, the present invention is a peeled black beans ( Glycine max Merr) provides a composition for promoting collagen production containing the extract as an active ingredient.

According to another aspect of the present invention, the present invention provides a cosmetic composition for preventing skin aging containing peeled black bean extract as an active ingredient.

Peeled black bean extract included as an active ingredient in the composition of the present invention is extracted from the kernel of black beans removed from the black beans.

The inventors of the present invention prepared extracts by various extraction methods using a plurality of natural products as a raw material, and as a result of performing the work of selecting substances excellent in antioxidant effect, collagen synthesis effect and skin wrinkle improvement effect, the peeled black soybean extract described above It was found that the best fit for the purpose.

On the other hand, according to the disclosure of the Republic of Korea Patent Application No. 1997-58585, it is known that there is an antioxidant, cell proliferative effect and skin color improvement effect when extracting the water-soluble components by using a common solvent extraction method as a cosmetic component.

However, the peeled black bean extract of the present invention is excellent in the collagen synthesis effect, which is a novel effect not found in the existing black bean extract, and the skin wrinkle improvement effect is excellent.

Peeled black soybean extract of the present invention can remove the cytotoxicity appearing in the black soybean hull, remove the pigments unique to the black soybean hull can extend the ease of application of the formulation, it is provided as an active ingredient of the cosmetic composition for preventing skin aging.

As used herein, the term "anti-aging of skin" has the meaning encompassing comprehensively the prevention and treatment of skin aging.

The extraction solvent used to prepare the peeled black bean extract is water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (e.g. methanol, ethanol, propanol and butanol, etc.), a mixed solvent of the lower alcohol and water, acetone, It is selected from the group consisting of ethyl acetate, butyl acetate, chloroform and 1,3-butylene glycol, preferably a hydrous lower alcohol, more preferably ethanol and most preferably 70% ethanol.

More specifically, the peeled black bean extract included in the composition of the present invention is prepared by the following manufacturing process: (a) washing and then peeling black beans to obtain only kernels and drying and crushing; (b) adding 70% ethanol and extracting at 15-35 ° C .; And (c) filtering the extract and drying.

On the other hand, it will be apparent to those skilled in the art that the peeled black bean extract of the present invention can obtain an extract having substantially the same effect using not only the aforementioned extraction solvent but also other extraction solvents.

In addition, the peeled black soybean extract of the present invention includes not only the extract by the aforementioned extraction solvent, but also an extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The active fraction is also included in the extract of the present invention.

Peeled black bean extract of the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure, freeze drying or spray drying.

According to a preferred embodiment of the present invention, the content of the extract is 0.0001-10.0% by weight based on the total weight of the composition. If the content is less than the expected effect is difficult to appear, if the content is exceeded the effect is no longer increased.

The ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions in addition to the extract as an active ingredient, and include, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, and Carrier.

Cosmetic compositions of the present invention may be prepared in any formulation conventionally prepared in the art and include, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, and especially in the case of spray, additionally chlorofluorohydrocarbon, Propellant such as propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The composition containing peeled black soybean extract of the present invention has an excellent antioxidative effect on lipid peroxidation, promotes collagen production, and has an excellent skin wrinkle improvement effect.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Preparation Example 1 Preparation of Peel Black Bean Extract

Black beans (Bioland, Korea) were washed with purified water and peeled to obtain only kernels. The peeled black beans were dried and broken up into small pieces, and then 1-10 times 70% ethanol of dry weight was added thereto. After extraction at 15-35 ° C. for 5 days, it was filtered through a 300 mesh filter cloth, and again filtered through a filter paper of Whatman 5, followed by drying with a rotary vacuum evaporator. The dried peeled black bean extract powder was used by dissolving in 50% 1,3-butylene glycol.

Preparation Example 2 Preparation of Black Bean Peel Extract

Black soybeans were washed with purified water, dried only in shells, broken into small pieces, and 70% ethanol 1-10 times the dry weight was added thereto. After extraction at 15-35 ° C. for 5 days, it was filtered through a 300 mesh filter cloth, and again filtered through a filter paper of Whatman 5, followed by drying with a rotary vacuum evaporator. The dried black bean husk extract powder was used by dissolving in 50% 1,3-butylene glycol.

Preparation Example 3 Preparation of Black Bean Extract

The black beans were washed with purified water, dried and broken into small pieces, and then 1-10 times 70% ethanol of dry weight was added thereto. After extraction at 15-35 ° C. for 5 days, it was filtered through a 300 mesh filter cloth, and again filtered through a filter paper of Whatman 5, followed by drying with a rotary vacuum evaporator. The dried black soybean extract powder was used by dissolving in 50% 1,3-butylene glycol.

Experimental Example 1 Cytotoxicity Inhibition Test of Peeled Black Bean Extract

Fibroblasts (Korea Cell Line Bank), which are human skin cells, were cultured in T-75 flasks (Falcon, USA) until 80% growth. This was again transferred to each well of a 96-well plate (Falcon, USA) to 10 ⁵ cell count / well and incubated for 24 hours. After incubation, the microscope checks whether cells grow in the well of the plate and adds 200 μl of DMEM (Sigma, United States) to each well, and then extracts of Preparation Examples 1, 2, and 3, respectively, 0.5, 1 Adjusted to 2 and 4 mg / ml. 10 μl of MTT ([3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) reagent was added to each well to compare the viability of the cells 24 hours after the addition of the test substance. . After 4 hours, the cell viability was measured by absorbance at 570 nm wavelength using an ELISA reader, and cell viability was calculated according to Equation 1 below.

% Cell viability = Test substance absorbance / control absorbance × 100

Cell viability Concentration of extract (mg / ml) Cell survival rate (%) Black Bean Extract Black Bean Peel Extract Peeled Black Bean Extract 0.5 92.0 73.8 100 One 79.7 50.6 100 2 65.2 42.9 100 4 48.3 17.5 100

Looking at the Table 1, it can be seen that the cell viability of the black soybean hull extract is the lowest, the cytotoxicity of the black soybean extract is represented by the shell, the peeled black soybean extract is much higher cell viability than the black soybean extract and black soybean extract It can be seen that it effectively suppresses. Accordingly, it can be seen that the peeled black bean extract is an extract having no cytotoxicity.

Experimental Example 2: Antioxidant Activity Test of Peeled Black Bean Extract

In order to confirm the antioxidant effect of the extract of Preparation Examples 1, 2 and 3 was measured the lipid peroxidation inhibitory effect using linoleic acid (linoleic acid). Linoleic acid was purchased from Sigma Co., Ltd, USA and used. First, 4.4 ml of Fenton's reagent (2% SDS, 0.79 mM potassium chloride, 0.25 mM Trizma base (pH 7.4), 1 μM FeCl ₂ , 0.025 mM H ₂ O ₂ ), 50 μl linoleic acid and extracts of Preparation Examples 1, 2 or 3 500 μl was mixed and reacted at 55 ° C. for 16 hours while blocking light. To compare the effect of inhibiting lipid peroxidation, 500 μl of antioxidant BHT (buthylated hydroxytoluene) was added instead of extracts. 2 ml of the reaction solution, 1 ml of 35% trichloroacetic acid, and 2 ml of 0.75% TBA were mixed and boiled for 10 minutes, followed by cooling. 5 ml of the reaction solution was mixed with 1 ml of acetic acid and 2 ml of chloroform, and the absorbance was measured at 530 nm.

Using the following equation 2 to obtain the lipid peroxidation inhibitory effect and the results are shown in Table 2 below.

Lipid Peroxidation Inhibitory Effect Concentration of extract (%) Lipid Peroxidation Inhibitory Effect (%) Black Bean Extract Black Bean Peel Extract Peeled Black Bean Extract BHT 0.025 18.3 1.1 67.3 51.4 0.05 36.1 3.5 78.1 61.5 0.1 55.3 6.2 91.6 73.3 0.2 69.7 9.4 94.7 80.4

As can be seen in Table 2, the peeled black soybean extract of the present invention is significantly improved the lipid peroxidation inhibitory effect compared to the conventional black bean extract and was superior to the lipid peroxidation inhibitory effect than the BHT used as an antioxidant previously.

Experimental Example 3: Test for promoting collagen production of peeled black bean extract

Collagen synthesis promoting effect was measured for the extract of Preparation Example 1-3. Black bean extract of Preparation Example 1-3 was used by dissolving to a suitable concentration in PBS buffer solution.

Human normal fibroblasts were seeded in 48-well microplates containing DMEM medium (Sigma Co., Ltd. USA) containing 10% fetal bovine serum (1 × 10 ⁵ cells / well ), And incubated at 37 ° C. for 24 hours in a 5% CO ₂ incubator. Normal human fibroblast cells were used to separate the skin tissue collected after circumcision of the newborn in the hospital. Serum-free DMEM medium was prepared so that the final concentrations of the extracts prepared in Preparation Examples 1-3 were 50, 100, and 200 μg / ml, and then the fibroblast culture medium was replaced with this medium for 24 hours and 48 hours. After culturing fibroblasts for hours and 72 hours, each cell culture solution was collected. In order to compare the collagen production effect, ascorbic acid, which is a type I collagen biosynthesis promoting raw material, was added instead of the extract as a comparative example.

The degree of collagen production promotion was confirmed by measuring the amount of procollagen type I C-peptide produced in the collected cell culture liquid using the collagen protein measurement kit of Takara Corporation (Takara Shuzo Co., Ltd. Japan). First, the collected cell culture solution was placed in a 96-well microplate uniformly coated with primary collagen antibody, and the antigen-antibody reaction occurred at 37 ° C. for 3 hours. Cell cultures in the wells were removed and washed four times with PBS buffer. After adding the color causing substance to each well for 15 minutes at room temperature, 1 N sulfuric acid solution was added to stop the reaction.

The absorbance at wavelength 450 nm was measured with a spectrophotometer. A standard curve was prepared using the standard solution, and the absorbance obtained by the above method was substituted into the standard curve to calculate collagen production amount of the cell culture solution to which each sample was added. Collagen synthesis promotion rate was calculated by the following equation 3 and the experimental results are shown in Table 3 below.

Collagen synthesis promotion rate (%) = [(A × 100) / B]-100

In the above formula, A is the collagen production amount of the sample addition culture solution, B is the collagen production amount of the culture solution without addition of the sample.

Collagen biosynthesis promoting effect Concentration of extract (µg / ml) Type I collagen production growth rate (%) Black Bean Extract Peeled Black Bean Extract Ascorbic acid 50 67.9 146.2 54.8 100 138.5 267.1 75.4 200 167.3 382.7 74.9

As shown in Table 3, the type I collagen biosynthesis effect of the peeled black bean extract was greatly improved than the black bean extract, and the concentration-dependent increase in type I collagen biosynthesis and ascorbic acid used as a raw material for promoting type I collagen biosynthesis At lower concentrations, collagen production increased. Therefore, the peeled black bean extract of the present invention was found to have an excellent collagen biosynthesis effect.

Based on the experimental example, the present invention proposes a cosmetic composition of various solubilizers and emulsifiers containing peeled black soybean extract with excellent antioxidant and collagen biosynthesis effects. Although this is described in detail in the following formulation example, the composition of the present invention is not limited only to the following examples.

Formulation Example 1: Softener (Skin Lotion)

To a softening lotion containing the peeled black bean extract of Preparation Example 1 in the composition of Table 4 was prepared according to a conventional method.

ingredient Content (% by weight) Peeled Black Bean Extract 2.5 glycerin 5.0 1,3-butylene glycol 3.0 PEG 1500 1.0 Allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaluronate 5.0 ethanol 10.0 Octicdodeceth-16 0.2 Polysorbate 20 0.2 incense 0.02 Uniside-U 13 0.01 Green # 13 0.001 Distilled water Remaining amount Sum 100

Formulation Example 2: Converging Cosmetic Water

To the composition of Table 5, astringent cosmetic water containing the peeled black bean extract of Preparation Example 1 was prepared according to a conventional method.

ingredient Content (% by weight) Peeled Black Bean Extract 2.5 glycerin 2.0 1,3-butylene glycol 2.0 Allantoin 0.2 DL-panthenol 0.2 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaluronate 3.0 ethanol 15.0 Polysorbate 20 0.3 Witch Hagel Extract 2.0 Citric acid a very small amount incense 0.02 Uniside-U 13 0.01 Green # 13 0.001 Distilled water Remaining amount Sum 100

Formulation Example 3: Nutrients

To nutrient cosmetics containing the peeled black bean extract of Preparation Example 1 in the composition of Table 6 was prepared according to a conventional method.

ingredient Content (% by weight) Peeled Black Bean Extract 2.5 Glyceryl Stearate SE 1.5 Cetearyl Alcohol 1.5 lanolin 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Cured Vegetable Oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoine 2.0 Dimethicone 0.8 Tocopherol Acetate 0.5 Carboxyvinyl Polymer 0.12 glycerin 5.0 1,3-butylene glycol 3.0 Sodium hyaluronate 5.0 Triethanolamine 0.12 incense 0.01 Uniside-U 13 0.02 Green # 3 0.003 Distilled water Remaining amount Sum 100

Formulation Example 4: Nutrition Cream

The nutrition cream containing the peeled black bean extract of Preparation Example 1 in the composition of Table 7 was prepared according to a conventional method.

ingredient Content (% by weight) Peeled Black Bean Extract 6.0 Lipophilic monostearate 2.0 Cetearyl Alcohol 2.2 Stearic acid 1.5 Beeswax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Cured Vegetable Oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoine 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaluronate 4.0 incense 0.01 Uniside-Y13 0.02 Green # 3 0.003 Distilled water Remaining amount Sum 100

Formulation Example 5: Massage Cream

A massage cream containing the peeled black bean extract of Preparation Example 1 was prepared according to the composition of Table 8 according to a conventional method.

ingredient Content (% by weight) Peeled Black Bean Extract 5.0 Lipophilic Monostearic Acid Glycerin 1.5 Cetearyl Alcohol 1.5 Stearic acid 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Isostearyl Isosterate 5.0 Squalane 5.0 Mineral oil 35.0 Dimethicone 0.5 Hydroxyethyl cellulose 0.12 glycerin 6.0 Triethanolamine 0.7 incense 0.01 Uniside-Y13 0.02 Green # 3 0.003 Distilled water Remaining amount Sum 100

Formulation Example 6: Essence

Essence containing the peeled black bean extract of Preparation Example 1 in the composition of Table 9 below was prepared according to a conventional method.

ingredient Content (% by weight) Peeled Black Bean Extract 5.0 glycerin 10.0 Betaine 5.0 PEG 1500 2.0 Allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl cellulose 0.1 Sodium hyaluronate 8.0 Carboxy Vinyl Polymer 0.2 Triethanolamine 0.18 Octyldodecanol 0.3 Octyldodeceth-16 0.4 ethanol 6.0 incense 0.02 Uniside-U 13 0.01 Green # 3 0.001 Distilled water Remaining amount Sum 100

Formulation Example 7: Pack

A pack containing the peeled black bean extract of Preparation Example 1 was prepared according to the composition of Table 10 according to a conventional method.

ingredient Content (% by weight) Peeled Black Bean Extract 2.5 Polyvinyl alcohol 15.0 Cellulose gum 0.15 glycerin 3.0 PEG 1500 2.0 Chicdestrin 0.15 DL-panthenol 0.4 Allantoin 0.1 Glycyrisin Monoammonium 0.3 Nicotinamide 0.5 ethanol 6.0 PEG 40 Cured Castor Oil 0.3 incense a very small amount Uniside-Y13 0.02 Green # 3 0.003 Distilled water Remaining amount Sum 100

Experimental Example 4: Determination of wrinkle improvement effect of cream containing peeled black bean extract

Wrinkle improvement effect of the present invention was measured in 20 healthy Korean women (age distribution 35-51 years). The cream of Formulation Example 4 containing the peeled black bean extract, Cream which replaced the peeled black bean extract with 0.1% by weight ascorbic acid in Formulation Example 4 (Comparative Example 1) and cream with 0.1% by weight retinoic acid (Comparative Example 2 ), And a cream (control) replaced with purified water was applied to the area of 2 × 2 cm 2 of the upper arm of the arm twice daily for morning and evening for 0.2 g for 6 weeks. Replica of skin wrinkles was floated before and after 8 weeks of application with a silicone solution, and the skin wrinkles were measured with a skin visiometer (Skin Visiometer SV400, C + K Electronics GmbH., Germany). The image of the replica was analyzed three-dimensionally by a CCD camera, and the average wrinkle roughness (R _z ) of Equation 4 below was obtained by dividing the sum of roughness (R _m : m is an integer of 1 or more) of each wrinkle by the number of wrinkles. Wrinkle improvement effect was analyzed. The experimental results are shown in Table 11.

Rz = (R ₁ + R ₂ + R ₃ + ... R + R _m-2 + R _m-1 + R _m ) / number of wrinkles (m)

division ΔRz = Rz before use-Rz after 6 weeks Control Formulation Example 4 Comparative Example 1 Comparative Example 2 Subject 1 0.005 0.151 0.105 0.130 Subject 2 0.009 0.150 0.116 0.116 Subject 3 0.006 0.145 0.106 0.124 Subject 4 0.003 0.149 0.115 0.118 Subject 5 0.006 0.132 0.108 0.102 Subject 6 0.008 0.139 0.116 0.104 Subject 7 0.014 0.145 0.124 0.109 Subject 8 0.011 0.147 0.110 0.127 Subject 9 0.010 0.142 0.127 0.134 Subject 10 0.006 0.137 0.124 0.126 Subject 11 0.008 0.156 0.121 0.105 Subject 12 0.006 0.142 0.122 0.116 Subject 13 0.011 0.135 0.135 0.128 Subject 14 0.009 0.143 0.116 0.120 Subject 15 0.010 0.150 0.134 0.127 Subject 16 0.012 0.147 0.128 0.132 Subject 17 0.006 0.129 0.113 0.127 Subject 18 0.007 0.137 0.123 0.113 Subject 19 0.003 0.156 0.119 0.127 Subject 20 0.001 0.140 0.112 0.118 Average 0.008 0.144 0.119 0.120

According to the results of Table 11, the control group containing only purified water did not show an anti-wrinkle effect, and Formulation Example 4 containing the peeled black bean extract contained Comparative Example 1 and retinoic acid containing ascorbic acid instead of the peeled black bean extract. The wrinkle improvement effect was much higher than the comparative example 2 which contained. Comparative Example 1 and Comparative Example 2 had a statistically significant difference in the 99% confidence level in the wrinkle improvement effect with Formulation Example 4. Therefore, it can be seen that the cosmetic composition containing the peeled black bean extract of the present invention as an active ingredient has an excellent skin wrinkle improvement effect.

As described in detail above, the present invention provides a cosmetic composition for preventing skin aging comprising peeled black bean extract as an active ingredient. Peeled black soybean extract has superior effects compared to the existing antioxidants and collagen synthesis agents, and has an excellent skin wrinkle improvement effect when manufactured and used in cosmetics containing the same as an active ingredient.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that the specific technology is merely a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (5)

Collagen production promoting composition comprising a peeled black bean extract as an active ingredient. A cosmetic composition for preventing skin aging containing the composition of claim 1 as an active ingredient.  The method according to claim 1 or 2, wherein the peeled black bean extract is water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, a mixed solvent of the lower alcohol and water, acetone, ethyl acetate, butyl acetate, chloroform and 1, A composition, characterized in that it is extracted using a solvent selected from the group consisting of 3-butylene glycol. The composition of claim 1 or 2, wherein the peeled black bean extract is contained in an amount of 0.0001-10.0% by weight based on the total dry weight of the composition. 3. The cosmetic of claim 2 wherein the cosmetic consists of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Cosmetic composition for preventing skin aging, characterized in that it has a formulation selected from the group.