KR20060016155A

KR20060016155A - An enzyme concerning in biosynthesis of sch47554 & sch47555 and the genes thereof

Info

Publication number: KR20060016155A
Application number: KR1020040064507A
Authority: KR
Inventors: 우진석; 송재경; 이희찬; 류광경; 정영수; 강선엽; 김대희; 서원민; 양지영
Original assignee: 주식회사 진켐
Priority date: 2004-08-17
Filing date: 2004-08-17
Publication date: 2006-02-22
Also published as: KR100564161B1

Abstract

본 발명은 Sch47554 및 Sch47555 생합성에 관여하는 효소 및 그 유전자에 관한 것으로, 더욱 상세하게는, Sch47554 및 Sch47555 생합성에 관여하는 유전자 클러스터의 염기서열, Sch47554와 Sch47555 생합성계 효소인 dNTP-4-케토-6-데옥시글루코스-2,3-디하이드라타아제 및 상기 효소를 코딩하는 유전자에 관한 것이다.The present invention relates to enzymes involved in Sch47554 and Sch47555 biosynthesis and genes thereof, and more specifically, to the base sequence of gene clusters involved in Sch47554 and Sch47555 biosynthesis, dNTP-4-keto-6 which is Sch47554 and Sch47555 biosynthetic enzymes. -Deoxyglucose-2,3-dihydratase and a gene encoding said enzyme.

본 발명에 따른 Sch47554 및 Sch47555 생합성에 관여하는 유전자 클러스터, dNTP-4-케토-6-데옥시글루코스-2,3-디하이드라타아제(SchS2) 및 그 유전자는 Sch47554, Sch47555 또는 이와 유사한 신규 앙구사이클린계 항생제의 제조에 유용하게 사용될 수 있다.Gene clusters involved in Sch47554 and Sch47555 biosynthesis according to the present invention, dNTP-4-keto-6-deoxyglucose-2,3-dihydratase (SchS2) and the genes thereof are Sch47554, Sch47555 or similar novel fungus It can be usefully used for the preparation of cyclin antibiotics.

Sch47554, Sch47555, 앙구사이클린, dNTP-4-케토-6-데옥시글루코스-2,3-디하이드라타아제Sch47554, Sch47555, angucycline, dNTP-4-keto-6-deoxyglucose-2,3-dihydratase

Description

Enzyme Concerning in Biosynthesis of Sch47554 & Sch47555 and the Genes Thereof

도 1은 Sch47554 및 Sch47555의 구조와 생합성 예상경로를 나타낸 것이다.Figure 1 shows the structure and predicted biosynthetic pathways of Sch47554 and Sch47555.

도 2는 Sch47554 및 Sch47555의 유전자 클러스터 지도를 나타낸 것이다. 화살표는 각 유전자들을 나타내고, 화살표의 방향은 전사방향을 나타낸다. 2 shows a gene cluster map of Sch47554 and Sch47555. Arrows indicate each gene and the direction of the arrow indicates the transcription direction.

도 3은 본 발명에 따른 SchS2 효소의 SDS-PAGE 분석결과를 나타낸 것이다. 레인 1은 표준분자량 마커를 나타내고 레인 2 및 3은 각각 soluble protein 및 insoluble protein을 나타낸다. Figure 3 shows the SDS-PAGE analysis of the SchS2 enzyme according to the present invention. Lane 1 represents standard molecular weight markers and lanes 2 and 3 represent soluble and insoluble proteins, respectively.

도 4는 표준 말톨(maltol)(A)과 SchS2 효소의 반응산물(B)에 대한 HPLC 분석결과를 나타낸 것이다. Figure 4 shows the HPLC analysis of the reaction product (B) of the standard maltol (A) and SchS2 enzyme.

본 발명은 Sch47554 및 Sch47555의 생합성에 관여하는 효소 및 그 유전자에 관한 것으로, 더욱 상세하게는, Sch47554 및 Sch47555 생합성에 관여하는 유전자 클러스터의 염기서열, Sch47554 및 Sch47555 생합성계 효소인 dNTP-4-케토-6-데옥시글루코스-2,3-디하이드라타아제 및 상기 효소를 코딩하는 유전자에 관한 것이다.The present invention relates to enzymes involved in the biosynthesis of Sch47554 and Sch47555, and more particularly, to the sequences of gene clusters involved in Sch47554 and Sch47555 biosynthesis, dNTP-4-keto- which are Sch47554 and Sch47555 biosynthetic enzymes. 6-deoxyglucose-2,3-dihydratase and a gene encoding said enzyme.

앙구사이클린 그룹의 항생제는 미생물에 의해 생산되어, 넓은 약학적 활성 및 생물학적 활성을 가지는 천연물질이다 (Rohr, J. et al., Nat. Prod. Rep., 9:103, 1992). 이들은 테트라싸이클릭 벤자안트라센(tetracyclic benz(a)anthracene) 아글리콘 시스템을 포함하며, 빠르게 개발되고 있는 새로운 항생제 중 하나이다 (Rohr, J., "Bioorganic Chemistry, Deoxysugars, Polyketides and related classes: Synthesis, Biosynthesis, Enzymes", Springer Verlag, Germany, 2000). Antibiotics of the angucycline group are produced by microorganisms and are natural substances with broad pharmacological and biological activities (Rohr, J. et al. , Nat. Prod. Rep., 9: 103, 1992). These include the tetracyclic benz (a) anthracene aglycone system and are one of the fastest growing new antibiotics (Rohr, J., "Bioorganic Chemistry, Deoxysugars, Polyketides and related classes: Synthesis, Biosynthesis, Enzymes ", Springer Verlag, Germany, 2000).

데카케타이드(decaketide) 방향족 탄소사슬은 폴리케타이드 경로를 거친 열개의 아세테이트 유닛의 비대칭적 조합에 의해 유도된다. 마크로라이드와는 달리, 상기 방향족 폴리케타이드는 탄소사슬을 완전하게 하도록 반복 사용되는 단일 또는 이중 기능의 개개 단백질로 구성된 타입 II PKS(polyketide synthase)에 의해 생합성된다 (Rawlings, B.J., Nat. Prod. Rep. 16:425, 1999).The decaketide aromatic carbon chain is derived by an asymmetric combination of ten acetate units via the polyketide pathway. Unlike macrolides, the aromatic polyketides are biosynthesized by type II polyketide synthase (PKS) consisting of individual proteins of single or dual function which are used repeatedly to complete the carbon chain (Rawlings, BJ, Nat. Prod. Rep. 16: 425, 1999).

Sch47554 및 Sch47555는 Strepomyces sp. SCC-21362에 의해 생성되는 2개의 새로운 앙구사이클린 항생제이다. 이들은 1992년에 처음으로 발견되었으며, Candida albus, C. tropicalis, C. Stellaoidea 등의 곰팡이에 대하여 활성을 나타내는 항진균제이다. 구조적으로, Sch47554 및 Sch47555는 다중방향성 앙구사이클린 아글리콘으로 구성되고, 데옥시당(DOH) 부분을 갖는, 아쿠아야마이신(aquayamycine) 및 어다마이신 A(urdamycin A)와 유사하다.Sch47554 and Sch47555 are described in Strepomyces sp. Two new angucycline antibiotics produced by SCC-21362. They were first discovered in 1992 and are antifungal agents that are active against fungi such as Candida albus, C. tropicalis and C. Stellaoidea . Structurally, Sch47554 and Sch47555 are similar to aquayamycine and auramycin A, which consist of multidirectional angucycline aglycones and have a deoxysaccharide (DOH) moiety.

Sch47554는 D-amicetose/L-aculose의 C-9 및 C-3 위치에 각각 C-glycosidated disaccharide 및 O-glycosidated L-aculose를 가지고 있다. Sch47555는 D-amicetose/L-amicetose의 C-9 및 C-3 위치에 각각 C-glycosidated L-aculose 및 O-glycosidated L-aculose를 가지고 있다 (도 1). 이러한 항생제들은 C-12b 위치 대신에 C-3에 O-glycosidated olicosaccharide를 가지고 있는 점에서 어다마이신 A와 다르다.Sch47554 has C -glycosidated disaccharide and O -glycosidated L-aculose at positions C-9 and C-3 of D-amicetose / L-aculose, respectively. Sch47555 has a D-amicetose / L-amicetose C -9 and C aculose -glycosidated L-O and L--glycosidated aculose each in C-3 position (Fig. 1). These antibiotics differ from adamycin A in that they have an O -glycosidated olicosaccharide at C-3 instead of the C-12b position.

지난 십년간 구조적으로 연관되고, 생물학적 활성을 가지는 앙구사이클린을 코딩하는 유전자 클러스터가 클로닝되고, 유전자 서열이 결정되었다. 이들 중에는 어다마이신(urdamycin) A, 랜도마이신 A(landomycin A), 자도마이신 B(jadomycin B), 오비에도마이신(oviedomycin) 및 PD116740과 같은 항생제의 클러스터와 시모사이클리논(simocyclinone), 그리세오로딘 A(griseorhodin A) 및 오리신(auricin) 등의 새로운 항생제 클러스터가 포함되어 있다 (Decker, H. and Hagg, S., J. Bacteriol., 177:6126, 1995; Westrich, L., FEMS Microbiol. Lett., 170:381, 1999; Han, L., Microbiol., 140:3379, 1994; Hong, S.T., J. Bacteriol., 179:470, 1997; Trefzer, A., Antimicrob. Agents Chemother., 46:1174, 2002; Galm, U., Arch. Microbiol., 178:102, 2002; Li, A. et al., J. Chem. Biol., 9:1017, 2002; Novakova, R., Gene, 297:197, 2002). 앙구사이클린을 생산하지 않는 균주인 Streptomyces sp. PGA64 균주에서도 다른 앙구사이클린 유전자 클러스터 와 높은 상동성을 가지는 타입 II PKS와 유사한 사일런트 유전자 클러스터가 클로닝된 바 있다. 이 유전자 패밀리에 속하는 다른 유전자 클러스터들이 계속적으로 발견되고 있다.Over the past decade, gene clusters that encode structurally related, biologically active angucyclines have been cloned and gene sequences have been determined. Among these are clusters of antibiotics such as uramyamy A, landomycin A, jadomycin B, obiedomycin, and PD116740, and simocyclinone, griseorodin A new antibiotic clusters such as griseorhodin A and aurinin (Decker, H. and Hagg, S., J. Bacteriol ., 177: 6126, 1995; Westrich, L., FEMS Microbiol. Lett , 170: 381, 1999; Han, L., Microbiol ., 140: 3379, 1994; Hong, ST, J. Bacteriol ., 179: 470, 1997; Trefzer, A., Antimicrob.Agents Chemother ., 46: 1174, 2002; Galm, U., Arch. Microbiol., 178: 102, 2002; Li, A. et al., J. Chem. Biol., 9: 1017, 2002; Novakova, R., Gene , 297: 197, 2002). Streptomyces sp., A strain that does not produce angucycline. In the PGA64 strain, a silent gene cluster similar to type II PKS, which has high homology with other angucycline gene clusters, has been cloned. Other gene clusters belonging to this gene family are constantly being found.

앙구사이클린 생산에 관여하는 유전자는 하이브리드의 조합을 이용하여 더 많이 분리될 수 있을 것이다. 폴리케타이드 경로의 유전적 조작은 이미 여러 실험실에서 행하여지고 있으며, 서로 다른 클러스터의 폴리케타이드 합성 도메인을 각각 조합하는 것도 가능할 것이다. 이들의 조합에 의해 활성 효소 복합체를 형성하고, 이들 하이브리드 PKS를 이종 숙주에서 발현시키면, 새로운 방향성 폴리케타이드를 생성할 수 있다. 다른 한편으로, 생물학적 활성을 조절하여 항생제를 인식하는 데옥시당의 구조를 변화시키고자 하는 노력이 계속되고 있다.Genes involved in the production of angucycline could be further separated using a combination of hybrids. Genetic manipulation of polyketide pathways has already been done in several laboratories, and it would be possible to combine the polyketide synthetic domains of different clusters respectively. Combinations of these form active enzyme complexes and when these hybrid PKSs are expressed in heterologous hosts, new aromatic polyketides can be generated. On the other hand, efforts continue to change the structure of the deoxysaccharides that recognize antibiotics by regulating biological activity.

Sch47554 및 Sch47555의 생합성 유전자 클러스터의 클로닝은 이들 항생제의 생합성에 관한 정보를 제공할 뿐만 아니라, 하이브리드 폴리케타이드 및 데옥시당 조합의 생합성에 대한 유전적 배경을 넓힐 수 있게 한다. Sch47554 및 Sch47555에서의 특별한 2,3,6-트리데옥시당의 존재는 생화학 및 분자수준의 연구에 있어서 매우 중요하다.Cloning of the biosynthetic gene clusters of Sch47554 and Sch47555 not only provides information on the biosynthesis of these antibiotics, but also broadens the genetic background of the biosynthesis of hybrid polyketide and deoxysaccharide combinations. The presence of special 2,3,6-trideoxysaccharides in Sch47554 and Sch47555 is very important for biochemical and molecular studies.

이에 본 발명자들은, Streptomyces sp. SCC-2136의 염색체 DNA에서 Sch47554 및 Sch47555의 생합성 유전자 클러스터로 예상되는 유전자 단편을 클로닝하여, 염기서열을 결정하고, Sch47554 및 Sch47555의 생합성에 관여하는 유전자를 발현시켜 효소활성을 확인하고 본 발명을 완성하게 되었다.The present inventors, Streptomyces sp. Cloning the gene fragments expected from the biosynthetic gene clusters of Sch47554 and Sch47555 on the chromosomal DNA of SCC-2136 to determine the sequencing, expressing genes involved in the biosynthesis of Sch47554 and Sch47555, confirming enzymatic activity and completing the present invention. Was done.

본 발명의 목적은 Sch47554 및 Sch47555 생합성에 관여하는 유전자 클러스터의 염기서열을 제공하는데 있다.It is an object of the present invention to provide base sequences of gene clusters involved in Sch47554 and Sch47555 biosynthesis.

본 발명의 다른 목적은 Sch47554 및 Sch47555 생합성계 효소인 dNTP-4-케토-6-데옥시글루코스-2,3-디하이드라타아제 및 이를 코딩하는 유전자를 제공하는데 있다.
Another object of the present invention is to provide dNTP-4-keto-6-deoxyglucose-2,3-dihydratase, which is a Sch47554 and Sch47555 biosynthetic enzyme, and a gene encoding the same.

상기 목적을 달성하기 위하여, 본 발명은 서열번호 1의 염기서열을 가지는 Sch47554 및/또는 Sch47555 생합성계 유전자 클러스터를 제공한다. In order to achieve the above object, the present invention has a nucleotide sequence of SEQ ID NO: 1 Sch47554 and / or Sch47555 biosynthetic gene clusters are provided.

본 발명에 있어서, 상기 유전자 클러스터는 Streptomyces sp. SCC-2136(ATCC55186) 유래인 것을 특징으로 할 수 있다.In the present invention, the gene cluster is Streptomyces sp. SCC-2136 (ATCC55186) It may be characterized by being derived.

본 발명은 또한, 서열번호 2의 아미노산 서열을 가지는 dNTP-4-케토-6-데옥시글루코스-2,3-디하이드라타아제를 제공한다.The present invention also provides dNTP-4-keto-6-deoxyglucose-2,3-dihydratase having the amino acid sequence of SEQ ID NO: 2.

본 발명은 또한, 상기 dNTP-4-케토-6-데옥시글루코스-2,3-디하이드라타아제를 코딩하는 유전자(schS2), 상기 유전자를 함유하는 재조합 벡터 및 상기 재조합 벡터로 형질전환된 박테리아를 제공한다. 본 발명에 있어서, 상기 유전자는 서열번호 1의 33361~34758 영역의 염기서열 또는 이와 상보적인 염기서열을 가지는 것을 특징으로 할 수 있다.The present invention also relates to a gene encoding the dNTP-4-keto-6-deoxyglucose-2,3- dihydratase ( schS2 ), a recombinant vector containing the gene, and a transformed vector with the recombinant vector. Provide bacteria. In the present invention, the gene may have a nucleotide sequence of the region 33361 ~ 34758 of SEQ ID NO: 1 or having a nucleotide sequence complementary thereto.

본 발명은 또한, 상기 형질전환된 박테리아를 배양하는 것을 특징으로 하는 dNTP-4-케토-6-데옥시글루코스-2,3-디하이드라타아제의 제조방법을 제공한다.The present invention also provides a method for producing dNTP-4-keto-6-deoxyglucose-2,3-dihydratase, which comprises culturing the transformed bacteria.

이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 예시하기 위한 것으로서, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지는 않는 것은 당업계에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

본 발명에서 사용된 Streptomyces sp. SCC-2136(ATCC55186)는 ISP2(Difco, USA) 고체 배지에서 배양하였으며, Streptomyces sp. SCC-2136의 염색체 DNA는 액체 YEME 배지(bacto-yeast extract 0.15g/l, bacto-peptone 0.25g/l, malt extract 0.15g/l, glucose 0.5g/l, sucrose 17g/l, MgCl₂·6H₂O(1M) 2.5㎖/l, glycine(20%) 3㎖/l)에서 3일간 배양하여 분리하였다. 고체배양과 액체배양은 모두 28℃에서 수행하였다. 유전자 조작과 재조합 플라스미드의 제조에는 E.coli XL1Blue MRF(Stratagene, USA) 를 사용하였다. 유전자 발현용 숙주로는 E.coli BL21(DE3)(Invirogen, USA)을 사용하였다. E.coli의 배양은 LB액체배지에서 37℃로 수행하였으며, 재조합 플라스미드의 선택마커로서 암피실린(100μg/ml) 및 아프라마이신(100μg/ml)을 사용하였다. 벡터로는 코스미드 라이브러리를 위하여 pOJ446(Invitrogen, USA)을 사용하였으며, E.coli에서의 유전자 발현을 위하여 pET-32a(Novagen, USA)를 사용하였다. PCR 단편을 클로닝하기 위하여는 pGEM-T easy 벡터(Promega)를 사용하였다. Streptomyces sp. Used in the present invention. SCC-2136 (ATCC55186) was incubated in ISP2 (Difco, USA) solid medium, Streptomyces sp. The chromosomal DNA of SCC-2136 was expressed in liquid YEME medium (bacto-yeast extract 0.15g / l, bacto-peptone 0.25g / l, malt extract 0.15g / l, glucose 0.5g / l, sucrose 17g / l, MgCl ₂ ₂ O (1M) was for 3 days, removed from the 2.5㎖ / l, glycine (20% ) 3㎖ / l). Both solid culture and liquid culture were performed at 28 ° C. E. coli XL1Blue MRF (Stratagene, USA) was used for the engineering and preparation of the recombinant plasmid. E. coli BL21 (DE3) (Invirogen, USA) was used as a host for gene expression. Incubation of E. coli was performed at 37 ° C. in LB liquid medium, and ampicillin (100 μg / ml) and apramycin (100 μg / ml) were used as selection markers of the recombinant plasmid. POJ446 (Invitrogen, USA) was used for the cosmid library, and pET-32a (Novagen, USA) was used for gene expression in E. coli . To clone the PCR fragment, pGEM-T easy vector (Promega) was used.

실시예 1. DNA 라이브러리 구축 및 코스미드 라이브러리 스크리닝Example 1 DNA Library Construction and Cosmid Library Screening

DNA 라이브러리를 구축하기 위하여, Streptomyces. sp. SCC-2136의 염색체 DNA를 MboI를 사용하여 절단한 다음, 35~45kb 크기의 단편을 취하여, BamHI과 HpaI으로 처리된 pOJ446과 라이게이션하였다. 라이게이션된 시료의 인비트로 팩키징은 GigapackIII XL(Stratagene, USA)을 사용하여 행하였으며, E.coli XL1Blue MRF로 형질전환시켰다.To build the DNA library, Streptomyces. sp. The chromosomal DNA of SCC-2136 was cleaved using Mbo I, and then a fragment of 35-45 kb was taken and ligated with pOJ446 treated with Bam HI and Hpa I. In vitro packaging of the ligated samples was done using GigapackIII XL (Stratagene, USA) and transformed with E. coli XL1Blue MRF.

Streptomyces. sp. SCC-2136의 염색체 DNA로부터 얻은 340bp의 dTDP-글루코스-4,6-디하이드라테이즈 유전자는 DW11(서열번호 3), DW32(서열번호 4)의 프라이머를 사용하여 증폭시켰다. 증폭된 PCR 산물은 pGEM-T easy 벡터에 라이게이션시켜 서열분석 및 염색체 라이브러리의 프로브로 사용하였다. Streptomyces. sp. The 340 bp dTDP-glucose-4,6-dihydratase gene obtained from the chromosomal DNA of SCC-2136 was amplified using primers of DW11 (SEQ ID NO: 3) and DW32 (SEQ ID NO: 4). The amplified PCR product was ligated to the pGEM-T easy vector and used as a probe for sequencing and chromosome library.

DW11(서열번호 3): 5'-CACTTCGGGGGCGAGTCGCACGT-3'DW11 (SEQ ID NO: 3): 5'-CACTTCGGGGGCGAGTCGCACGT-3 '

DW32(서열번호 4): 5'-GGGCCGTAGTTGTTCGAGCA-3'DW32 (SEQ ID NO: 4): 5'-GGGCCGTAGTTGTTCGAGCA-3 '

프로브는 32P로 표지된 랜덤 프라이머 라벨링 키트(Stratagene, USA)를 사용하여 표지하였으며, 젤 여과법을 사용하여 정제하였다. Probes were labeled using a 32P labeled random primer labeling kit (Stratagene, USA) and purified using gel filtration.

하이브리다이제이션은 50℃에서 6시간동안 10㎖의 2X SSC용액에서 수행하였다. 콜로니 하이브리다이제이션과 써던 하이브리다이제이션은 Hybond N 나일론 막(Amersham, 독일)을 이용하여 수행하였다.Hybridization was performed in 10 ml of 2 × SSC solution at 50 ° C. for 6 hours. Colony hybridization and Southern hybridization were performed using a Hybond N nylon membrane (Amersham, Germany).

스크리닝 결과, 12개의 양성 코스미드 클론을 수득하였으며, 이중 하나의 클론을 선택하여 pSCC-1으로 명명하고 서열분석을 수행하였다. DNA 서열분석은 디데 옥시 체인 터미네이션법을 사용하여 자동 서열기에서 수행하였으며, 결정된 서열은 DNASIS(버젼 2.1, 히타치 소프트웨어, 일본)를 이용하여 정리하였으며, BLAST(http://www.ncbi.nlm.nih.gov/BLAST/)를 사용하여 상동성을 검색하였다.The screening resulted in 12 positive cosmid clones, one of which was named pSCC-1 and subjected to sequencing. DNA sequencing was performed in an automated sequencer using dideoxy chain termination method, and the determined sequences were arranged using DNASIS (version 2.1, Hitachi Software, Japan) and BLAST (http: //www.ncbi.nlm. homology was searched using nih.gov/BLAST/).

pSCC-1은 42.9kb의 단편이 삽입된 재조합 플라스미드로, 30개의 예상 ORF를 가지고 있고, DOH 생합성 유전자 및 두개의 PKS 관련 유전자를 포함하고 있으며, PKS 유전자는 DOH 클러스터의 upstream에 위치한다는 것을 알 수 있었다. 염색체상의 워킹(walking)에 의하여 업스트림 쪽으로 확장하여, 55개의 ORF를 가진 34.6kb의 서열을 분석하였다 (서열번호 1). 그 결과, 본 발명에 따른 유전자 클러스터는 입증된 타입 II PKS 및 여러 Streptomyces spp.의 데옥시당 생합성 유전자 클러스터와 광범위한 유사성을 나타내었다. 상동성 검색에 의해 확인된 본 발명에 따른 모든 유전자의 방향성과, 유전자 지도 및 예상되는 기능을 표 1과 도 2에 나타내었다.pSCC-1 is a recombinant plasmid with a 42.9 kb fragment, which has 30 expected ORFs, contains a DOH biosynthesis gene and two PKS-related genes, and the PKS gene is located upstream of the DOH cluster. there was. Extended upstream by walking on the chromosome, a sequence of 34.6 kb with 55 ORFs was analyzed (SEQ ID NO: 1). As a result, the gene cluster according to the present invention showed extensive similarity with the proven type II PKS and the deoxysaccharide biosynthetic gene cluster of several Streptomyces spp. The orientation, gene map and expected function of all the genes according to the invention identified by homology search are shown in Table 1 and FIG. 2.

서열번호 1의 염기서열에서 전체 55개 ORF의 영역은 다음과 같다: schA1 [498~1481]; schA2 [1515~2402]; schA3 [complement, 4587~5666]; schA4 [complement, 6218~6883]; schA5 [7072~7965]; schA6 [8041~9921]; schA7 [10223~11704]; schA8 [11716~12678]; schA9 [complement, 13077~13670]; schA10 [complement, 13683~14561]; schA11 [complement, 14577~15980]; schA12 [complement, 16240~17175]; schA13 [complement, 17172~18848]; schA14 [complement, 18845~20005]; schA15 [complement, 20025~22100]; schA16 [22247~23140]; schA17 [complement, 23237~24619]; schA18 [complement, 25015~26022]; schA19 [complement, 26093~27883]; schP1 [complement, 28019~29872]; schP2 [complement, 30033~31604]; schS1 [complement, 32312~33334]; schS2 [complement, 33361~34758]; schS3 [complement, 35871~37175]; schS4 [complement, 37175~38134]; schS5 [complement, 38166~39152]; schS6 [complement, 39149~40216]; schS7 [complement, 40301~41437]; schS8 [complement, 41434~42024]; schS9 [complement, 42086~43258]; schS10 [complement, 43298~44590]; schA20 [complement, 44623~45846]; schP3 [complement, 45969~47792]; schP4 [complement, 47970~48908]; schP5 [complement, 48935~49735]; schP6 [complement, 49768~50037]; schP7 [complement, 50097~51323]; schP8 [complement, 51320~52582]; schP9 [complement, 52606~52932]; schP10 [complement, 52990~54474]; schP11 [complement, 54705~55580]; schA21 [complement, 55722~56477]; schA22 [56595~57194]; schA23 [57209~58792]; schA24 [58880~59998]; schA25 [60242~61093]; schA26 [complement, 62333~63931]; schA27 [complement, 64202~64954]; schA28 [65981~66589]; schA29 [66593~67477]; schA30 [67521~69065]; schA31 [complement, 69585~70373]; schA32 [complement, 70497~72167]; schA33 [complement, 72198~73847]; 및 schA34 [complement, 74161~77076].The region of all 55 ORFs in the nucleotide sequence of SEQ ID NO: 1 is as follows: schA1 [ 498-1481 ]; schA2 [ 1515-2402 ]; schA3 [complement, 4587-5666 ]; schA4 [complement, 6218-6883 ]; schA 5 [7072 ~ 7965]; schA6 [ 8041-9921 ]; schA7 [10223-11704]; schA 8 [11716-12678]; schA9 [complement, 13077-13670 ]; schA10 [complement, 13683-14561]; schA11 [complement, 14577-15980 ]; schA12 [complement, 16240-17175]; schA13 [complement, 17172-18848]; schA14 [complement, 18845-20005]; schA15 [complement, 20025-22100]; schA16 [22247-23140]; schA17 [complement, 23237-24619]; schA18 [complement, 25015-26022]; schA19 [complement, 26093-27883]; schP1 [complement, 28019-29872]; schP2 [complement, 30033-31604]; schS1 [complement, 32312-33334 ]; schS2 [complement, 33361-34758]; schS 3 [complement, 35871-37175]; schS 4 [complement, 37175-38134]; schS5 [complement, 38166-39152]; schS6 [complement, 39149-40216]; schS7 [complement, 40301-41437]; schS8 [complement, 41434-42024]; schS9 [complement, 42086-43258]; schS10 [complement, 43298-44590]; schA20 [complement, 44623-45846]; schP3 [complement, 45969-47792]; schP4 [complement, 47970-48908]; schP5 [complement, 48935-49735]; schP6 [complement, 49768-50037]; schP7 [complement, 50097-51323]; schP8 [complement, 51320-52582]; schP9 [complement, 52606-52932]; schP10 [complement, 52990-54474]; schP11 [complement, 54705-55580]; schA21 [complement, 55722-56477]; schA 22 [56595-57194]; schA 23 [57209-58792]; schA24 [58880-59998]; schA25 [60242-61093]; schA26 [complement, 62333-63931]; schA27 [complement, 64202-64954]; schA 28 [65981-66589]; schA 29 [66593-67477]; schA30 [67521-69065]; schA31 [complement, 69585-70373]; schA32 [complement, 70497-72167]; schA 33 [complement, 72198-73847]; And schA 34 [complement, 74161-77076].

실시예 2. Sch47554 및 Sch47555 생합성 유전자 클러스터의 분석Example 2. Analysis of Sch47554 and Sch47555 Biosynthetic Gene Clusters

실시예 1의 서열분석과 상동성 분석을 통해, 본 발명에 따른 유전자 클러스터가 Sch47554 및 Sch47555의 생합성을 위한 유전자를 코딩한다는 것을 확인할 수 있었다. PKS 및 DOH 유전자는 같은 전사방향을 가지고 있었다. schS3-schS4, schS5-schS6, schS7-schS8 및 schP7-schP8의 시작코돈과 정지코돈은 겹쳐져 있어, 트랜스래이션 커플임을 알 수 있었다. schS1, schS4, schS7 및 schP8은 특이하게 GTG를 시작코돈으로 사용하고 있었다. PKS 및 DOH 유전자 클러스터는 트랜스포터 유전자에 의하여 분리되어 위치하고 있으며, 측면에는 여러 악세사리 유전자가 위치하고 있었다.Through sequencing and homology analysis of Example 1, it was confirmed that the gene cluster according to the present invention encodes genes for biosynthesis of Sch47554 and Sch47555. PKS and DOH genes had the same transcription direction. The start and stop codons of schS3 - schS4 , schS5 - schS6 , schS7 - schS8, and schP7 - schP8 overlapped, indicating that they were translated couples. schS1, schS4, schS7 and schP8 specifically used GTG as a start codon. The PKS and DOH gene clusters were separated by transporter genes, and various accessory genes were located on the side.

(1) 앙구사이클린 부분(moiety)의 형성에 관여할 것으로 예상되는 유전자(1) Genes expected to be involved in the formation of angucycline moiety

PKS 유전자는 schP3 -schP10에 의해 코딩된다. 이들 유전자들은 어다마이신 A 및 랜도마이신 A의 해당 유전자와 아미노산 서열과 유전적 조합에 있어서 매우 유사하다. 클러스터 중 가장 작은 ORF는 90개의 아미노산을 코딩하는 schP6이고, 이 유전자는 PKS-ACP와 상동성을 나타내며, 포스포판테틴(phosphopantethine)의 결합활성을 나타내는 ⁴³Ser 잔기를 포함하는 높은 보존적 모티브인 ³⁹LGYESLALLE⁴⁹를 포함한다. schP7의 시작코돈은 schP8의 정지코돈과 겹쳐, 타입II PKS의 특징인 CLF/KS 쌍을 나타낸다. schP8의 아미노 말단의 촉매 도메인은 활성 ¹⁷²Cys 잔기를 가진 보존구역인 ¹⁶⁶TVVSTGCTSGLD¹⁷⁷을 가지고, 카르복실기 말단부분에는 아실사슬과 결합하는 활성 ³⁵⁰Ser과 함께 AT 도메인을 나타내는 ³⁴⁸GHSLG³⁵²(GHSXG)를 가진다.The PKS gene is encoded by schP3 - schP10 . These genes are very similar in genetic combination with the corresponding genes of adamicin A and randomin A and the amino acid sequence. The smallest ORF in the cluster is schP6 , which encodes 90 amino acids, which is homologous to PKS-ACP and is a highly conservative motif containing ⁴³ Ser residues that indicate the binding activity of phosphopantethine. ³⁹ LGYESLALLE ⁴⁹ . start codon and stop codon of schP8 schP7 is overlaid, it indicates the characteristic of CLF / KS pairs of Type II PKS. the catalytic domain of the amino terminus of schP8 has a retention zone of ¹⁶⁶ TVVSTGCTSGLD ¹⁷⁷ with active ¹⁷² Cys residues, there has ³⁵² (GHSXG) ³⁴⁸ GHSLG representing the AT domain with the activity ³⁵⁰ Ser, which combined with the acyl chain carboxyl-terminal portion.

schP4, schP5 및 schP9는 각각 초기 폴리케타이드 사슬의 변형에 관여하는 아로마타아제(aromatase), 케토리덕타아제(ketoreductase) 및 싸이클라아제(cyclase)를 코딩하고 있다. schP4의 DNA 서열에서 추정되는 산물은 첫 번째 링의 아로마제이션에서 중요한 역할을 하는 효소와 상동성을 나타내었다. 싸이클라아제인 SchP9는 안트라싸이신보다 앙구사이클린 싸이클라아제와 유사하였다 (Fernandez-Moreno, M.A., et al., J. Biol. Chem., 267:19278, 1992). schP4 , schP5 and schP9 encode aromatase , ketoreductase and cyclase , respectively, involved in the modification of the initial polyketide chain. The putative product of the DNA sequence of schP4 showed homology with the enzyme, which plays an important role in the aromatization of the first ring. The cyclase SchP9 was more similar to angucycline cyclase than anthracycin (Fernandez-Moreno, MA, et al ., J. Biol. Chem. , 267: 19278, 1992).

schP5의 서열에서 추정되는 산물은 UrdD와 88%의 높은 상동성을 나타내었을 뿐만 아니라, 오비에도마이신 및 자도마이신과 같은 여러 앙구사이클린의 케토리덕타아제와도 상동성을 나타내었다. SchP5의 아미노기 말단에는 NAD(P)H의 결합 도메인인 ¹⁸GATSGIG²⁴를 가지고 있었다. 이들 케토리덕타아제는 다른 방향성 PKS 케토리덕테아제와 높은 상동성을 가지고, 초기 폴리-β-케톤사슬의 C-9 카르보닐 그룹의 부위 특이적인 환원에 관여하고 있다 (Hopwood, D.A., Chem. Rev., 97:2465, 1997). The presumed product in the sequence of schP5 showed a high homology of 88% with UrdD, as well as the keratoductases of several angucyclines, such as obiemycin and jadomycin. The amino group terminal of SchP5 had ¹⁸ GATSGIG ²⁴ which is a binding domain of NAD (P) H. These ketoductases have high homology with other aromatic PKS ketoductases and are involved in site-specific reduction of the C-9 carbonyl group of the initial poly-β-ketone chain (Hopwood, DA, Chem). Rev. , 97: 2465, 1997).

모듈라 PKS와 다르게, 앙구사이클린 유전자 클러스터의 가장 중요한 성질은 많은 산화효소(oxygenase)가 존재한다는 것이며, 예상에 맞게 본 발명의 유전자 클러스터는 2개의 산화효소가 schP3 및 schP10에 의해서 코딩되고 있었다. schP10은 링 B의 산화적 오프닝에 관여하는 산화효소인 UrdE 및 JadF(Jad-ORF6)과 상동성을 나타내었다. 최근, NADH 및 FAD에 의존적인 모노옥시게나아제(MtmOIV)가 미스라마 이신(mithramycin) 생산균주로부터 정제되었으며, 링의 오프닝에서 Baeyer-Villiger 타입 산화기작을 담당하고 있다. 다른 유전자들과 마찬가지로, SchP10도 아미노기 말단에 FAD 결합도메인을 가지고 있었으며, 카르복실기 말단에는 산소 결합도메인을 가지고 있었다. 한편, schP3은 UrdM의 어다마이신 A의 생합성에서의 역할과 마찬가지로, C-12b위치에 수산기를 첨가하는 역할을 수행한다. 이는 산화-환원효소이고, 아미노기 말단에는 산소 결합도메인을, 카르복실기 말단에는 NAD(P)H 결합도메인을 가진다.Unlike the modular PKS, the most important property of the angucycline gene cluster is the presence of many oxygenases. As expected, the gene cluster of the present invention was encoded by two oxidases by schP3 and schP10 . schP10 showed homology with UrdE and JadF (Jad-ORF6), the oxidases involved in the oxidative opening of ring B. Recently, monooxygenase (MtmOIV), which is dependent on NADH and FAD, has been purified from mithramycin producing strains and is responsible for Baeyer-Villiger type oxidation in the opening of the ring. Like other genes, SchP10 had an FAD binding domain at the amino end and an oxygen binding domain at the carboxyl end. On the other hand, schP3 plays a role of adding a hydroxyl group at the C-12b position, similarly to the role of UrdM in the biosynthesis of adamycin A. It is an oxidoreductase, and has an oxygen binding domain at the amino group end and a NAD (P) H binding domain at the carboxyl end.

Sch47554 및 Sch47555의 코어 PKS는 C₂₀ 사슬로 구성되고, 아세틸- 및 9개의 말로닐-CoA 확장자에 의해 조합되기 때문에 대사적 말로닐-CoA가 필수적이다. 아세틸-CoA의 카르복실레이션은 말로닐-CoA를 생산하기 위해 첫 번째로 행하여지는 단계이며, 이 단계는 JadJ와 상동성을 나타내는 Sch 유전자 클러스터의 schP1에 의해 촉매된다. 이 그룹의 단백질은 종종 ACC(Acetyl-CoA Carboxylase)라는 약자로 표시되며 이중적인 기능을 가진다. 이러한 카르복실라아제는 FAS(fatty acid synthases)와 자주 혼용된다. 그러나, JadJ는 FAS와 혼용되지 않으며, 대신 기능적으로 PKS와 연관되어 있다 (Han, L. et al., Microbiol., 146: 903, 2000). 인접한 schP2 또한 PKS의 전구체를 생산하는 유전자와 연관이 있으며, 상기 schP2는 말로닐-CoA 디카르복실라아제를 코딩하고, JadN 및 LanP와 상동성이 있었다.Metabolic malonyl-CoA is essential because the core PKS of Sch47554 and Sch47555 is composed of C ₂₀ chains and is combined by acetyl- and 9 malonyl-CoA extensions. Carboxylation of acetyl-CoA is the first step to produce malonyl-CoA, which is catalyzed by schP1 of the Sch gene cluster showing homology with JadJ . Proteins in this group are often abbreviated as ACC (Acetyl-CoA Carboxylase) and have a dual function. Such carboxylase is often mixed with fatty acid synthases (FAS). However, JadJ is not mixed with FAS but instead functionally associated with PKS (Han, L. et al ., Microbiol ., 146: 903, 2000). Adjacent schP2 is also associated with a gene that produces a precursor of PKS, which schP2 encodes malonyl-CoA decarboxylase and was homologous to JadN and LanP.

아글리콘 생합성에 의하면, 아세틸-CoA에 의한 사슬의 시작에 의해 코어구조가 형성되고, 9개의 말로닐-CoA가 추가되어 데카케타이드를 형성한다. SchP6, SchP7 및 SchP8은 상기 데카케타이드를 만들고, SchP4는 사슬을 방향성으로 만든다. SchP5는 특이적 케토리덕션을 보조하고, SchP9는 결정화를 촉매하고, SchP10은 마지막 링을 분해시킨다. SchP3는 C-12에 하이드록시기를 덧붙인다. 이와 같이 Ppost PKS 테일러링(tailoring) 효소들은 방향족 아글리콘을 생물학적으로 활성을 띠는 대사산물로 전환하는 역할을 하며, 옥시게네이즈 및 글리코실트랜스퍼라아제의 기능은 앙구사이클린에 있어서 중요한 역할을 한다 (Motamedi, H., et al., J. Bacteriol., 178:5243, 1996).According to aglycon biosynthesis, the core structure is formed by the start of the chain by acetyl-CoA, and nine malonyl-CoA are added to form decaketide. SchP6, SchP7 and SchP8 make up the decaketide, and SchP4 makes the chain aromatic. SchP5 assists in specific chelation, SchP9 catalyzes crystallization, and SchP10 degrades the final ring. SchP3 adds a hydroxyl group to C-12. As such, Ppost PKS tailinging enzymes convert aromatic aglycones into biologically active metabolites, and the function of oxygenase and glycosyltransferases plays an important role in angucyclines ( Motamedi, H., et al ., J. Bacteriol ., 178: 5243, 1996).

(2) 데옥시당의 형성에 관여할 것으로 예상되는 유전자(2) Genes expected to be involved in the formation of deoxysaccharides

데옥시당 생합성 유전자 클러스터는 schS1-schS10에 의하여 코딩된다. schS5 및 schS6로부터 예상되는 단백질은 각각 dNDP-글루코스-4,6-디하이드라타아제 및 dNDP-글루코스 신세이즈를 코딩하는 단백질들과 상동성을 나타내었다. 이들은 dNDP-4-케토-6-데옥시글루코스를 생산하는데 있어서, 첫 번째 두단계를 촉매하는 역할을 한다. 세 번째 단계는 dNTP-4-케토-6-데옥시-D-글루코스-2,3-디하이드라타아제에 의한 C-2의 데옥시게네이션이다. schs2 유전자의 산물은 어다마이신 A 생합성에서 L-로디노즈(rhodinose) 및 D-올리보스(olivose)의 생합성에 관여하는 UrdS와 80%의 상동성을 나타내었다. 또한, Gra-ORF27과도 상동성을 나타내었으며, 상기 단백질은 그라나티신(granaticin) 생합성에서 D-올리보스 및 L-로디노스의 생합성에서 C2-H를 유지시켜 C-2 디옥시게네이션에 관여한다고 밝혀졌다 (Spines, C.E. et al., J. Am. Chem. Soc., 101:701, 1979; Draeger, G. et al., J. Am. Chem. Soc., 121:2611, 1999). 그러므로, SchS2는 2,3-디하이드라타아제의 기능을 가진다고 볼 수 있다. Gra-ORF27에 의해 촉매되는 반응의 높은 반응성을 가지는 산물(dTDP-3,4-디케토-2,6-디데옥시-D-글루코스)은 Gra-OPR26(3-케토리덕타아제)에 의해 안정한 산물(말톨)로 전환된다. 그러므로, SchS2의 C-2 디옥시게네이션은 Gra-ORF26과 상동성을 가지는 SchS1(3-케토리덕타아제)에 의해 보조되는 것으로 추정된다. 그러므로, Sch47554 및 Sch47555의 모든 당 부분의 생합성에서 dTDP-4-케토-2,6-디데옥시글루코스가 중간산물이 된다. 이후 나머지 단계는 C-3 데옥시게네이션, 에피머제이션 및 4-케토리덕션이다. 스트렙토마이세스 속에서 수많은 디데옥시당 유전자가 클로닝되었지만, 트리데옥시당 유전자의 클로닝은 소수에 불과하며, 높은 정도의 데옥시게네이션은 생합성 경로에서의 유전자의 기능을 복잡하게 한다. The deoxysaccharide biosynthetic gene cluster is encoded by schS1-schS10 . The proteins expected from schS5 and schS6 showed homology with the proteins encoding dNDP-glucose-4,6-dihydratase and dNDP-glucose synthase, respectively. They catalyze the first two steps in producing dNDP-4-keto-6-deoxyglucose. The third step is deoxygenation of C-2 by dNTP-4-keto-6-deoxy-D-glucose-2,3-dihydratase. The product of the schs2 gene showed 80% homology with UrdS, which is involved in the biosynthesis of L-rhodinose and D-olivose in aadamine A biosynthesis. It also showed homology with Gra-ORF27, and said protein is involved in C-2 deoxygenation by maintaining C2-H in biosynthesis of D-olivose and L-rhodinos in granatitic biosynthesis. (Spines, CE et al ., J. Am. Chem. Soc. , 101: 701, 1979; Draeger, G. et al ., J. Am. Chem. Soc. , 121: 2611, 1999). Therefore, SchS2 can be considered to have the function of 2,3-dihydratase. The highly reactive product of the reaction catalyzed by Gra-ORF27 (dTDP-3,4-diketo-2,6-dideoxy-D-glucose) is stable by Gra-OPR26 (3-ketorductase). Converted to the product (maltol). Therefore, the C-2 deoxygenation of SchS2 is presumed to be assisted by SchS1 (3-ketoductase) having homology with Gra-ORF26. Therefore, dTDP-4-keto-2,6-dideoxyglucose is an intermediate in the biosynthesis of all sugar moieties of Sch47554 and Sch47555. The remaining steps are then C-3 deoxygenation, epimerization and 4-ketification. Although numerous dideoxy sugar genes have been cloned in Streptomyces, the cloning of trideoxysaccharide genes is a minority, and a high degree of deoxygenation complicates the function of genes in the biosynthetic pathway.

schS3, schS4 및 schS8의 산물은 여러 데옥시당 생합성 유전자의 아미노산 서열과 높은 상동성을 가지고, 각각 NDP-글루코스-3-디하이드라타아제, NDP-글루코스-4-케토리덕타아제 및 NDP-글루코스-3-(3,5) 에피머라아제의 기능을 가진다. schS7은 어다마이신 A 생합성에서 D-올리보스 경로의 C-C 결합의 C-9 글리코실레이션에 관여하는 UrdGT2와 71%의 높은 상동성을 가지고 있었다. 그러므로, SchS7은 Sch47554 및 Sch47555의 C-9에서 D-아미세토스(amicetose)를 전이시키는 C-글리코실트랜스퍼라아제이다. schS9에 의해 코딩되는 O-글리코실트랜스퍼라아제는 UrdGT1b/c의 아미노산 서열과 상동성을 나타내는 반면, O-글리코실트랜스퍼라아제 SchS10은 UrdGT1a와 상동성을 가지고 있었다. 그러므로, schS9 및 schS10은 Sch47554 및 Sch47555의 C-O 결합경로에서 L-아미세토스 및 L-아큘로스(aculose)의 전이를 담당하는 것으로 추정된다.The products of schS3 , schS4 and schS8 have high homology with the amino acid sequences of several deoxysaccharide biosynthetic genes, NDP-glucose-3-dehydratase, NDP-glucose-4-ketoductase and NDP-, respectively. Has the function of glucose-3- (3,5) epimerase. schS7 had 71% homology with UrdGT2, which is involved in C-9 glycosylation of CC bonds of the D-olivose pathway in adamycin A biosynthesis. Therefore, SchS7 is a C -glycosyltransferase that transfers D-amicetose at C-9 of Sch47554 and Sch47555. O -glycosyltransferase encoded by schS9 showed homology with the amino acid sequence of UrdGT1b / c, while O -glycosyltransferase SchS10 had homology with UrdGT1a. Therefore, schS9 and schS10 are presumed to be responsible for the transfer of L-Amicetose and L-Aculose in the CO binding pathways of Sch47554 and Sch47555.

이상에서와 같이, Sch47554 및 Sch47555의 폴리케타이드 및 데옥시당 유전자는 어다마이신 A의 생합성 유전자와 매우 높은 상동성을 나타내었고, 랜도마이신 A, 자도마이신 B, PD 117640, 시모사이클리논 및 오비에도마이신과 같은 앙구사이클린 합성 유전자와도 상동성을 가지고 있었다. sch PKS 유전자인 schP9, P8, P7, P6 및 P5는 어다마이신 A의 urdF, A, B, C, D 및 L, 자도마이신 B의 jadI, A, B, C, E 및 D와 랜도마이신 A의 lanF, A, C, D 및 F 등의 앙구사이클린 항생제의 PKS를 코딩하는 유전자와 높은 상동성을 나타내었다. Sch47554 및 Sch47555 유전자 클러스터와 어다마이신 유전자들과의 상동성은 88~48% 범위였다. 이중 어다마이신 유전자와 비교적 낮은 상동성을 보이는 유전자는 schS4(NDP-글루코스 4-케토리덕타아제, UdrZ3와 51% 상동성), schS9(O-글리코실트랜스퍼라아제, UrdGT1b/c와 53%와 상동성), schS10(O-글리코실트랜스퍼라아제, UrdGT1a와 48%의 상동성), schA20(UrdJ2와 52% 상동성) 및 schA23(UrdJ와 33%의 상동성)이었다.As described above, the polyketide and deoxysaccharide genes of Sch47554 and Sch47555 showed very high homology with the biosynthetic gene of adamycin A, and randomycin A, jadomycin B, PD 117640, sicyclinone and obiedo It also had homology with angucycline synthetic genes such as mycin. The sch PKS genes schP9 , P8 , P7 , P6 and P5 are urdF , A, B, C, D and L of adamycin A, jadI, A, B, C, E and D of jadomycin B and of randamycin A It showed high homology with genes encoding PKS of angucycline antibiotics such as lanF, A, C, D and F. Homology of the Sch47554 and Sch47555 gene clusters with the adamic genes ranged from 88-48%. Among the genes that showed relatively low homology with the adamamycin genes were schS4 (51% homology with NDP-glucose 4-ketoductase, UdrZ3 ), schS9 (O-glycosyltransferase, UrdGT1b / c and 53%). Homology), schS10 (O-glycosyltransferase, 48% homology with UrdGT1a), schA20 (52% homology with UrrdJ2 ) and schA23 (33% homology with UrrdJ).

어다마이신 A 유전자 클러스터는 L-로디노스 및 D-올리보스 생합성에 관여하는 UrdZ3 및 UrdR의 2,6-디데옥시당 및 2,3,6-트리데옥시당에 대한 두개의 서로 다른 4-케토리덕타아제를 가지고 있다. 4-케토-2,3,6-트리데옥시당에서 4-케토 그룹의 환원에 관여하는 SchS4는 UrdZ3와 낮은 상동성을 나타내고, 이는 UrdZ3와 SchS4의 입체특이성이 다르다는 증거가 될 수 있다. 유전자 클러스터에서 D- 및 L-아미세토스의 형성은 단일 SchS4에 의해서 설명할 수 있다.The adamycin A gene cluster has two different 4-kees for the 2,6-dideoxysaccharides and 2,3,6-trideoxysaccharides of UrdZ3 and UrdR involved in L-rodinose and D-olibos biosynthesis. It has toriductase. SchS4 involved in the reduction of the 4-keto group in 4-keto-2,3,6-trideoxysugars has low homology with UrdZ3, which may be evidence that the stereospecificity of UrdZ3 and SchS4 is different. The formation of D- and L-amicetose in the gene cluster can be explained by a single SchS4.

Sch47554 및 Sch47555 생합성에서 세 개의 다른 데옥시당 부분이 전이되는 것은 본 발명의 유전자 클러스터에 존재하는 세개의 글리코실트랜스퍼라아제에 의해 설명될 수 있다. SchS7과 다른 C-글리코실트랜스퍼라아제의 높은 상동성은 이들 글리코실트랜스퍼라아제가 2,3,6-트리데옥시당뿐만 아니라 2,6-디데옥시당을 전이한다는 것을 나타낸다. 게다가, C-글리코실트랜스퍼라아제는 뉴클레오티드 농도에 따라 상기 두 종류의 데옥시당을 전이할 수 있다는 보고가 있었다 (Hoffmeister, D. et al., Chem. Biol., 7:821, 2000). 어다마이신 O-글리코실트랜스퍼라아제와 SchS9 및 SchS10의 낮은 상동성으로 이들이 구조적으로 다른 데옥시당을 전이한다는 것을 알 수 있다. SchS9은 UrdGT1b 및 UrdGt1c와 상동성(~53%)을 나타내고, D-올리보실(C-9, 말단) 및 L-로디노실(rhodinosyl)의 전이(C-9 중간)에 관여한다. 한편, SchS10은 URdGT1a와 상동성(48%)을 가지고, D-로디노실 전이(C-12b)에 관여한다. 상기 결과들로, SchS9가 Sch47555의 C-9에서 D-아미세토실(amicetosyl) 전이에 관여하고, SChS10은 Sch47554 및 SCh47555에서 C-3 및 C-9에서 L-아큘로실(aculosyl)의 전이에 관여할 것이라고 추정된다. The transfer of three different deoxysaccharide moieties in Sch47554 and Sch47555 biosynthesis can be explained by the three glycosyltransferases present in the gene cluster of the invention. The high homology of SchS7 with other C -glycosyltransferases indicates that these glycosyltransferases transfer not only 2,3,6-trideoxy sugars but also 2,6-dideoxy sugars. In addition, it has been reported that C -glycosyltransferases can transfer both kinds of deoxysaccharides depending on nucleotide concentrations (Hoffmeister, D. et al ., Chem. Biol ., 7: 821, 2000). The low homology of adamycin O-glycosyltransferase with SchS9 and SchS10 shows that they transfer structurally different deoxysaccharides. SchS9 shows homology (-53%) with UrdGT1b and UrdGt1c and is involved in the transition of D-olibosyl (C-9, terminal) and L-rhodinosyl (middle of C-9). SchS10, on the other hand, has homology with URdGT1a (48%) and is involved in D-rodinosyl transition (C-12b). These results indicate that SchS9 is involved in D-amicetosyl transition in C-9 of Sch47555 and SChS10 is in transition of L-aculosyl in C-3 and C-9 in Sch47554 and SCh47555. It is estimated to be involved.

(3) 다른 기능의 유전자들(3) genes of different functions

본 발명에 따른 Sch47554 및 Sch47555의 생합성 유전자 클러스터에는 5개의 조절유전자와 6개의 트랜스포트 유전자 및 4개의 산화환원 기능을 가지는 유전자가 존재하였다. schA4 및 schA21은 몇몇의 Streptomyces PKS 클러스터에서 확인된 TeR-패밀리 조절 유전자에 속하였다. SchA25는 랜도마이신 A의 생합성에서 양성 조절자의 역할을 하는 LanI과 58%의 상동성을 나타내었다 (Rebets, Y. et al., FEMS Microbiol. Lett., 222, 149, 2003). 전사 조절자인 SchA27은 S. coelicolor 염색체에서 발견된 전사조절인자와 78%의 상동성을 나타내었다. In the biosynthetic gene clusters of Sch47554 and Sch47555 according to the present invention, there were five regulatory genes, six transport genes, and genes having four redox functions. schA4 and schA21 belonged to the TeR-family regulatory genes identified in several Streptomyces PKS clusters. SchA25 showed 58% homology with LanI, which acts as a positive regulator in the biosynthesis of randamycin A (Rebets, Y. et al. , FEMS Microbiol. Lett ., 222, 149, 2003). SchA27, a transcriptional regulator, showed 78% homology with the transcriptional regulator found on the S. coelicolor chromosome.

schS1, schA2, schA3, schA7, schA20 및 schA23은 저항성을 나타내는 기능을 하는 단백질들을 코딩하였다. schS1 및 schA2는 박테리아에서 퍼미아제(permeases) 패밀리에 속하며, schA3 및 schA7은 아미노산트랜스포터에 속하였다. schA20 유전자는 어다마이신 A 생합성 유전자 클러스터에서 PKS 및 DOH 유전자 사이에 존재하는 urdJ2와 상동성을 나타내고, 마찬가지로 PKS 및 DOH 유전자 사이에 위치하고 있었다. 또 다른 트랜스포터인 SchA23은 양자 의존성 트랜스포터로 알려진 urdJ/lanJ와 상동성을 나타내었다 (Westrich, L. et al., FEMS Microbiol. Lett., 170:381, 1999; Faust, B. et al., Microbiol., 146:147, 2000). schS1, schA2, schA3, schA7, schA20 and schA23 coded proteins that function as resistant. schS1 schA2 and belongs to the Fur Mia (permeases) family from bacteria, schA3 and schA7 has belonged to the amino acid transporter. The schA20 gene showed homology with urdJ2 present between the PKS and DOH genes in the adamycin A biosynthetic gene cluster and was similarly located between the PKS and DOH genes. Another transporter, SchA23, showed homology with urdJ / lanJ, known as a quantum dependent transporter (Westrich, L. et al ., FEMS Microbiol. Lett ., 170: 381, 1999; Faust, B. et al . , Microbiol. , 146: 147, 2000).

Sch47554 및 Sch47555의 생합성 과정동안, schA20은 자가 내성을 조절한다. schP11는 PKS 클러스터에 인접하여 있으나, 이의 유전자 산물은 앙그사이클린 생합성 유전자 중 어떤 유전자와도 의미있는 상동성을 나타내지 않았으며, 단지 기능이 밝혀지지 않은 Med-ORF27과 42%의 상동성을 나타내었다.During the biosynthesis of Sch47554 and Sch47555, schA20 regulates self tolerance. schP11 is adjacent to the PKS cluster, but its gene product showed no significant homology with any of the angcyclin biosynthetic genes, and only 42% homologous to Med-ORF27, a function of which is not known. .

실시예 3. 발현 플라스미드의 구축 및 형질전환Example 3. Construction and Transformation of Expression Plasmids

1389bp의 schS2를 프라이머 S2-I과 S2-II를 사용하여 코스미드 pSCC-1으로부터 증폭시켰다. 증폭된 단편을 BamHI과 EcoRI으로 절단하여 pET-32a(+)(Novagen, USA)에 클로닝하여 재조합플라스미드 pSCHS2를 제작하였다. 상기 pSCHS2를 E. coli BL21(DE3)에 도입하여 재조합 대장균을 수득하였다.1389 bp schS2 was amplified from cosmid pSCC-1 using primers S2-I and S2-II. The amplified fragment was digested with Bam HI and Eco RI and cloned into pET-32a (+) (Novagen, USA) to produce recombinant plasmid pSCHS2. The pSCHS2 was introduced into E. coli BL21 (DE3) to obtain recombinant E. coli.

S2-I(서열번호 5):5'-GGATCCCATATGCTGTCCCACACCCTT-3'S2-I (SEQ ID NO: 5): 5'- GGATCC CATATGCTGTCCCACACCCTT-3 '

S2-II(서열번호 6): 5'-GAATTCCCGCGCTCAACGTGCCCT-3'S2-II (SEQ ID NO: 6): 5'- GAATTC CCGCGCTCAACGTGCCCT-3 '

실시예 4. Example 4. Sch2Sch2 의 발현과 SCH2 효소의 활성분석Expression and SCH2 Enzyme Activity Analysis

pSCHS2로 형질전환된 E. coli BL21(DE3)을 2.5mM 베타인(betaine) 및 1M 솔비톨이 첨가된 LB 배지에서 배양하였다. 상기 배양액을 100㎖의 LB 배지로 옮겨 37℃에서 배양하여, 배양액의 흡광도가 OD₆₀₀에서 0.6에 도달하였을 때, 최종농도가 0.4mM이 되도록 IPTG를 첨가하여 schS2의 발현을 유도한 후, 22℃에서 32시간 추가로 배양하였다. 상기와 같이 schS2의 발현이 유도된 E. coli BL21(DE3)/pSCHS2를 SDS-PAGE로 확인한 결과, 68.2kDa의 재조합 단백질을 확인할 수 있었다 (도 3). E. coli BL21 (DE3) transformed with pSCHS2 was incubated in LB medium supplemented with 2.5 mM betaine and 1 M sorbitol. After culturing the culture solution at 37 ℃ transferred to LB medium 100㎖, when the absorbance of the culture solution reached 0.6 OD at _600, by the addition of IPTG at a final concentration of 0.4mM to induce expression of schS2, 22 ℃ Incubated for an additional 32 hours. As described above, E. coli BL21 (DE3) / pSCHS2 induced schS2 expression was identified by SDS-PAGE. As a result, 68.2 kDa recombinant protein was confirmed (FIG. 3).

SchS2의 효소활성은 in vitro 효소분석을 통하여 측정하였다. 효소분석을 위한 반응 혼합물은 전체부피 100㎕에 50mM 트리스-HCl(pH7.4) 버퍼, 5.0mM의 기질(dTDP-4-케토-6-데옥시글루코스) 및 30㎕ 세포질 Sch2가 포함된 용액을 사용하였다. 상기 반응 혼합물을 37℃에서 3시간 동안 반응시키고, 95℃에서 30초동안 열처리하여 반응을 종료시켰다. 원심분리에 의해 단백질을 제거하고, 상등액을 얻어 반응산물의 분석에 사용하였다. SchS2에 의한 반응산물인 말톨(maltol)을 역상 HPLC로 분석함으로서 효소활성을 측정하였다. 즉, SchS2의 반응산물은 260nm 파장에서 UV 검출기를 통해 HPLC를 사용하여 분석하였다. 칼럼은 Mightysil RP-18, GP250-4.5(5μM)를 사용하였으며, 1% HCOOH를 포함한 증류수에서 0-25%의 CH₃CN 그래디언 트로 30분이상 용출하였으며, 유속은 1.0ml/분이었다.Enzyme activity of SchS2 was determined by in vitro enzyme analysis. The reaction mixture for enzymatic analysis was prepared with a solution containing 50 mM Tris-HCl (pH 7.4) buffer, 5.0 mM substrate (dTDP-4-keto-6-deoxyglucose) and 30 µl cytoplasmic Sch2 in 100 µl total volume. Used. The reaction mixture was reacted at 37 ° C. for 3 hours and heat treated at 95 ° C. for 30 seconds to terminate the reaction. Protein was removed by centrifugation, supernatant was obtained and used for analysis of the reaction product. The enzyme activity was measured by analyzing maltol, a reaction product by SchS2, by reversed phase HPLC. That is, the reaction product of SchS2 was analyzed using HPLC through a UV detector at 260 nm wavelength. The column used Mightysil RP-18, GP250-4.5 (5μM), eluted with 0-25% CH ₃ CN gradient in distilled water containing 1% HCOOH more than 30 minutes, flow rate was 1.0ml / min.

HPLC 분석결과, 반응산물(말톨)의 피크는 15.3분에서 관찰되었으며, 대조군의 단일 말톨로 행한 분석결과와 일치하였다 (도 4). 이 결과로부터 본 발명 따른 SchS2 효소는 dTDP-4-케토-6-데옥시글루코스-2,3-디하이드라타아제 활성을 가진다는 것을 확인할 수 있었다.As a result of HPLC analysis, the peak of the reaction product (maltol) was observed at 15.3 minutes, and was consistent with the analysis result of a single maltol of the control group (FIG. 4). From this result, it was confirmed that the SchS2 enzyme according to the present invention has dTDP-4-keto-6-deoxyglucose-2,3-dihydratase activity.

이상으로 본 발명 내용의 특정한 부분을 상세히 기술하였는 바, 당업계의 통상의 지식을 가진 자에게 있어서, 이러한 구체적 기술은 단지 바람직한 실시양태일 뿐이며, 이에 의해 본 발명의 범위가 제한되는 것이 아닌 점은 명백할 것이다. 따라서 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의하여 정의된다고 할 것이다. Having described the specific part of the present invention in detail, it is obvious to those skilled in the art that such a specific description is only a preferred embodiment, thereby not limiting the scope of the present invention. something to do. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

본 발명은 Sch47554 및 Sch47555 생합성에 관여하는 유전자 클러스터의 염기서열, Sch47554 및/또는 Sch47555 생합성계 효소인 dNTP-4-케토-6-데옥시글루코스-2,3-디하이드라타아제(SchS2) 및 이를 코딩하는 유전자를 제공하는 효과가 있다.The present invention relates to sequences of gene clusters involved in Sch47554 and Sch47555 biosynthesis, dNTP-4-keto-6-deoxyglucose-2,3-dihydratase (SchS2), which are Sch47554 and / or Sch47555 biosynthetic enzymes, and It has the effect of providing a gene that encodes it.

<110> GENECHEM INC. <120> An Enzyme Concerning in Biosynthesis of Sch47554 & Sch47555 and the Genes Thereof <130> P04-312 <160> 6 <170> KopatentIn 1.71 <210> 1 <211> 77550 <212> DNA <213> Streptomyces sp. SCC-2136 <400> 1 actagtggat catgtcgttc ttcgcgcagg acgcgacggc gatcaaggac atgcagctgc 60 tgcgcggcat cccgccgacg gagaaggccc gcaacctgat cgcctcgggc ctgaccccga 120 cggacaagct gaacatggag aacgccgact acatctccca gcgcgtcggg aaggcgacca 180 acgccctgcc cgcgccggtg ccgccgccgc agggtgccga ccagatctgg gacctgctgt 240 tccagtcgaa cctcgcggtg gccttcggca agaagtcgat caagggccag ctcggggagt 300 tcttcgacca ggcgtcgggg atcctgggcg gctgacgctc cgcgtcggcg ggcgtgtcgt 360 ccggaagccg cggaccggcc gtggctggtc gcgcccacgc ggcgagccac atatcgacac 420 agccccgcgc ccctgaaggg gcacggcagc cgccgagtag tgccgcacca ccgcaagacc 480 gggagagggc cagggagatg agtatgacca ccgcgaagac agacgaggtc gtgaaggagc 540 agcccgctcc ctcgaccccg tccgaccagc gggagcgcga ccgtatgcgc cgccgcctcg 600 ccagacggcg gggcgggggc tggtggccgt acgccttcct ggccccctgg ttcatcggcc 660 tgttcggcct gacgatctac ccgatgctgg actcgctgta tctgtccttc accaacttcg 720 acctgctgac tccggcgcag tgggtcggga ccgacaacta cagcaagatg ttcaccgacg 780 acccggtctt ctgggactcg gtgcacgcca cgctgctgta cgtcgtcgtc tcggtgccgc 840 tgaagctcgc gctcgcgctc ggtgtggcga tgctgctcaa ccgcgacctg aagggcatcg 900 gtctgtaccg ggccgccttc tatctgccgt cgctgctcgg cggggcggtc gccatcgcca 960 tcgtgtggcg gcaggtcttc ggcggcgacg gcctgttcaa cgacttcctc gcctggttcg 1020 gcatcaaggg ccaggactgg atctccagcc ccgacaccgc gctctacacg ctgatcctgc 1080 tggccgtctg gcagttcggc accccgatgg tcatcttcct cgcgggtctg aagcagctgc 1140 cgaaggatgt gtacgaggcc gccgcgatcg acggcgcctc gccggtcacc cggttcttca 1200 agatcaccct gccgctgctg accccgatcg tcttcttcaa cgtggtcctg cagatcatcg 1260 acgcgttcaa gacgttcacc cccgccttcg tcatcagcaa cggctccggc ggaccgctca 1320 actcgacgat gctgtactcc ctctacctgt acaaaaaggg cttcaccgac ctccagatgg 1380 gatacgcctc ggctctcgcc tgggtgctgt tcctggtcat cgctggcttc acggcggtca 1440 acttcatcgc cagccgctac tgggtccact acgacgactg acgcctgacg tccgagcgag 1500 gagtccttca gcccatgtcc gccatcacct cggcccacgc cccgaagtcg ccgttgcaga 1560 agctgcgttc ggccaccgga tcgcgccgca tcttcatcca caccgtgctc atcggcgtgg 1620 cgatcgtcat gctctatccg ctgctgtgga tgctgagcag ctcgttcaag cccgacaccc 1680 atatcttcac ccagccgggg ctcatcccga gctcggtgcg cccggagaac tactccgagg 1740 gctggagcgg ctcgggcaac tccttctccc tctacatcac caactcgctg atcgtcacga 1800 tcggcgcggt gatcgggaac gtgatctcct gctcgctggc cgcctacgcc ttcgcgcgct 1860 tcgagttccg cggcaagaag atctggttcg gcctgatgct cggcacgctg atgctgccca 1920 cccaggccgt gctgatcccg cagtacacga tcttctacaa cctgacctgg atcaacacct 1980 atctgccgct gatcgtgccg aagttcctcg cggtggacgc cttcttcatc ttcctgatgg 2040 tccagttcat ccgctccatc ccgcgcgagc tcgaccacgc ggcgatgatg gacggcgcca 2100 accccttcca gatctactgg aagatcatcc tgccgctgat gaagccggcc ctgatcacca 2160 ccacgatctt caccttcatc tggacgtacg acgacttcct ccaccagctc gtctacctcc 2220 agcagaacga caagttcacc gtccccctcg gcctgaccct gttcatggac cagacgagcg 2280 gctcctccta cggtgcgatg ttcgccatgt cgactcttgc cctcctgccc acgctcatct 2340 gcttcctcat cttccagaag aggctggtag aaggcttggc gacctcgggc atgaagggct 2400 gaatcccgtg catcttcccc accagcgcgg ccagttgcac cacctccccg acgacccggc 2460 cgcgtacgac gcagtgctgg ccgccgtcac cgagacggcc ctgacccgcc tcacccccga 2520 gggcaacctc gagaacccgg ccgccgtcga cgacatcggc gacacctcgc tcggcatcac 2580 gtccctgctc gcgctcgcct ggcaccgcac caaggaccca cggctgcccg aagcggtccg 2640 ccgcagcctc gccttccacc tgaactcgcg cgtctacacc gaggacaacc ccggctaccc 2700 gaacctgacg gcacgcaact cccgtctgcc gtacgcccgt tacacgctgg aggcaggcgc 2760 gcaccccatc ggcgactggc cgagcacggt gtgggcgctg ctccaggccg tcaacgtcct 2820 cgacgcggcg gacggcctcg tcgacgacga gcagtaccgc gaactcctgg acgtcgcggg 2880 cggttactgg cgctggctca ccgaggccac cttcttcaac ccgcaggacg cggcgaacca 2940 ggccatcggc tgcgtggtcg gcggcctgat gctcgcccgc catctcccgc ccgaggaggg 3000 cgagtcggtc cggcggcgtg ctctgcagct gtacgccgac gagatccgcg cccggcgtgt 3060 acgggaccgg ggcgcgcccc tgcctccgga gcacggcggc gcctacgaca acaactacgg 3120 gcccatctcc ctgtccttcc tcgcgcaggc ccaccgggtc agcggcgagg aggtcttcgc 3180 ggaggacggc gacacgctcg cccggtacat cgacgcgcgg atctgcgcga gcggcttcga 3240 catcggcggg ccgcgctaca gcgagcagca ctccgccttc gaatccgtcc tcgggctgcg 3300 gtacttcggc catcgcatcg gcgccgacat cggtcgctac gccggtgact cgcgctgggc 3360 ccggcacgcg gtcggggccg acggcggcgt ggacgggcac ttcgcgtaca tgctcgtctg 3420 gcagatccag gacaccacga gctggcaccg cacgccgagc acggcgcccg cccgccacca 3480 gctgcggtcc ggcacggtca ccgtgtcgtt cgacgaccgg atgacgccct ccgtgatcga 3540 ggccggcggc acggcgctcg tggccgccgc cgtcaaccgg cagcacgggt tcgggcccgt 3600 gacggacgac ttcctgctgt gccgtccgat gggggcggta cgggtggcgg acgtgcgcgc 3660 cgacgggctc gcggccaagc tcgtgaccaa gcccgtcgtc ggacgtgacc atgtcctgcg 3720 ccggcttcgg tcgctgtacg tcacggacgg cacgcggctg tgggccaccg tggtcgtcga 3780 acggctcgac ggggagcggt acttgctcgc cgggatgccg tatgtcgccg cggacggtga 3840 ccggttccgc cgggtgggcg acttcccgct cgtcgccgcc gccggcgagc tgcgcctgac 3900 gcaccccccg gaaggcagcc cggactcctt cgacgcccgc gccgaagtca cccagcagca 3960 ggccgcgttc gcgttggcgg ccgatccgcg tgggtacggg gaccccgacg ccgggtggcg 4020 gcacgtgcgg accacgtccg cgctggaggt cgggcccgtg ccggatgcgc cggaggggct 4080 cgacgtcttc gcggtgcggt acggggaacc ggccgccgag ccgctgaccg tggtgtgcga 4140 gcgggtggag gaggggctgg ttctgcgtac gggctccttc accgcggtgc tcggcgaagc 4200 cgacgagcat ggcgagccgc agctcacgtt gactcccgcg gggacctgac ctcctcggcg 4260 gatgcgcccg agcctccgga cagcttttgc tgcccggggg ctcgggcgtt ccggagggat 4320 ttcgcccccg ccgcccctac cctcccccac tctcggcttc gctcgagcgg ggggaccccc 4380 atcgtccctt actcaggggc tccgcccccg aacccccgcc aaaagattgc gcagttcccc 4440 gcgcccctgg gtttctaggg gcgcggggaa ctgcgcggcc agcaacagcc gacctccacc 4500 cgccaagaca ccccgcgggg tctggggcgg agccccagaa ggacgggaat gggtaggggc 4560 ggcgggggcg aaagagggtt ggtgggtcag gccgagaccg gctctcggtt cggggagtcc 4620 tggggtgccc gctcgccgag gtgttccgtc gggaccttgt gggtttcgcg ggccgtgagc 4680 gccgcgatca ccggcgggac gcacagcgcc gcggtgaaca gcgccaccga cgcccagtcg 4740 tccccgtccg gacccgcgat ctgcgcggcg aaggtcaccg cgaacccggc gatggcgaag 4800 ccgatctgcg tgccgatggc catgcccgag aggcggaccc gagtggagaa catctcgccg 4860 tagaaggacg gccagacacc gttcgcggcg ctgtagacga caccgaaggt gacgatgccg 4920 aggaccatga ccagcgggta ggagcccgtg gagatggccc acaggtaggc gaccatcagc 4980 acgccactgc ccgcggcgcc gatcaggaag acgggccggc ggccgatgcg gtcggagagg 5040 gttgcccaga gggggatggc gcccagggcg accaggttgg cgagcgcacc cacccacagc 5100 atggacgtac ggtccatgcc gacggagtcg ctggtggcgt acgacagcgc ccacaccgtg 5160 aagatcgtgc tgaccgaggc gacgagcgcg gccgcgacca ccctgaggac gtccgcccag 5220 tggtcccgca gcagcacggc gagcggcatc ttcgcgacgc cctcggtggc ggcctgctgg 5280 gtgaaggtcg gggtctcctc cagcgtgcgg cggatgacgt agccgacgac ggcgaccgcg 5340 atgctcatcc agaacgggac ccgccagccc caggagagga gttggtcctc ggggagcgcg 5400 gcgaccggga tgaacaccag cgtggcgagg agctggccgc cctgcgtgcc gctgagcgtg 5460 aagctggtga agaagccgcg tttgttgggt ggcgcgtgtt ccagtgacat ggagttggcg 5520 ctggcctgct cgccggcggc cgagatgccc tgcaggacac ggcagatcac caggagcacc 5580 ggggccagcg tgccgacctg gtcgcgcgtc ggcagacagc cgatgaggaa cgtcgagagg 5640 cccatcagga tcagcgtgaa gaccatgatc ttcttgcggc cgaccttgtc gccgaagtgc 5700 ccgaggaaca gcgcgccgac cggcctggcc gcgtacgcga cgccgaacgt ggccagcgac 5760 agcagggtcg cggtggccgg gtccgactcg tcgaagaaga cctcggggaa gatcagcgcc 5820 gccgcgctgc cgtagatgaa gaagtcgtag tactccagcg cgctgccgat ccaggcggcc 5880 gtcgcagcct tctttggctg gccgggtggc gcgtcgaggg gcgctgcggg gacggacacg 5940 gcttgctcct tcgggggaac tccaacgtaa gcggagtgag cggaggggga gaagtgccgg 6000 atccctgggg tgatggggcg gccgtgccgg gggctacccg gctaattaac ccactggata 6060 gttagtagcg gttggctacg gatgttgcgc tcacgtttcc cgggtgtcaa gaggccgtac 6120 cgaaggatct tgcggtgccg ttcccgcgtc ccgcccgcac cgtgccgggc ccgcatcgcg 6180 ccgcgccgag ctttcagtcc gtcgagcctt cgggctctca gtccgccgag cggtccgccg 6240 tcagataggc gatgaccatg tcgcccagca tgttccggta gtgctcgcgc tgggccgggt 6300 cgaccaggtc gcggccgaac agggccccga aggtgtgccg gttggcgacc cggaagaagc 6360 agaacgagct gatcatcgcg tgcaggtcga cggcgtccac gtcggcggtg aagaggcccg 6420 actcctgccc cgactccagg atgcggcgga tcacgtccag ggcgggcgag ccgatcttgc 6480 cgagcttctc ggaggcggcg acgtgctcgg caccgtggat gttctcgatg ctgaccaggc 6540 ggatgaagtc ggggtgcgcc tcgtggtggt cgaaggtcag ctcggccagc cgccggatgg 6600 ccgcgaccgg gtccaggtgc tccacgtcga gcccctgctc ggcctcgcgg atgacgctgt 6660 acgcccgctc cagtaccgcg gtgaacagct ggtccttgcc gccgaagtag taatagatca 6720 tccgcttcgt ggtccgggtg cgggcggcga tctcgtcgac cctggccccg tcgtagccgg 6780 cccgggcgaa ctcgtgcgtg gcgacgtcga ggatctcggc ctgggtgcgg gccgcgtcac 6840 ggatgcgccc gttcggtcgt gccggttctt cgacgctggt catcgggctt ccttcgggcg 6900 aggggcgtgt gcccgagatt ctagaagcag gcttccgcgc acggttccgg gttacgggcg 6960 tgcacggttc cgggttgtcg gccgggcctt cccgtggctc gcggatgctg gtatagctaa 7020 cgtactagtt cgtacattag tgagccgctc tggaggtccg tcgtggtcgc catggtcaag 7080 gactcgtatc tcgtcgggct gatcggcgcc gggatcggcc cgtcgctcag cccggcactg 7140 cacgagcggg aggccgaccg gcagggcctg cgctatctgt accggctgat cgacatcgac 7200 gcgctcggtg tcgggccgca ggcggtgggg gacctcgtac gagccgcccg cgacctgggc 7260 ttcgacgggc tgaacatcac gcatccctgc aagcagctcg tcatcgggca tctggacgcg 7320 ctcgccccgc aggccgaggc gctcggcgcg gtgaacaccg tcgtcttcga gggcgggcgt 7380 gcggtcgggc acaacaccga tgtcaccggg ttcgccgcct cgttcgcccg tgggctgccg 7440 gatgccccgc tggagcgggt cgtgcagttg ggcgcggggg gagcgggggc ggccgtcgcg 7500 catgccatgc tcacgctcgg ggccgggcac gtcaccgtcg tcgatgccat gccggaccgg 7560 tcggcggacc tcgccgcctc gctgaaccgg cacttcggtg cggggcgggc cgctgccgcg 7620 ggcccggagc ggctggcggc gctgctcggc ggtgcggacg gcatcgtgca tgccacgccg 7680 acggggatgg ccgctcatcc ggggctgccg cttcccggtg agttgctgca tcccgggttg 7740 tgggtggccg aggtggtgta ccggccgttg gagaccgagt tgctgcgtgc cgctcgggcg 7800 gcggggtgtg cggttctcga tggtgggggg atggctgttt tccaggccgc ggacgcgttt 7860 cggctgttca cggggcggga gccggacgcg gtgcggatgc ttgcggatat tgcggagttg 7920 gccggtgtgg ccggtgccgc aggcgccgcc ggggcgcgga gttgagggcg tctcgggggt 7980 gaggggcttg gacggtgcgg gcccttcggg gttgatcgcg cagttccccg cgccccttgg 8040 atgcagggcc tcagccgtgt cgtgaagtgg aaccggagga gcatcaacgt gcgtacgtcc 8100 attgccaccg tctctctcag tggggccctt accgagaagc tcacggccgc ggcgcgggcg 8160 ggcttcgacg gtgtcgagat cttcgagaac gacctgctcg ccagccccct cacgcccgag 8220 gaggtgcggg cccgctgtgc cgatctcggg ctgagcatcg acctctacca gccgatgcgt 8280 gacatcgagg cggtgccggc ggaggggttc gggcggaatc tgcggcgggc cgagcacaag 8340 ttccggctga tggagcggct cggggccgac accgtgctcg tgtgctccag cgtgtcgccg 8400 ctcgccgagg acgacgacgc gctggccgcg ttccagctgc ggcggctcgc cgaactggcc 8460 gaggggttcg gcatccgcgt cgcgtacgag gcgttggcgt gggggcggca cgtcagtacg 8520 tacgaccatg cctggcgcat cgtcgaggcg gccgaccatc ccgcgctcgg gacgtgcctg 8580 gacagttttc acatcctggc ccgcggttcc gatcccaagg ggatcgagga cattcccggc 8640 gagaagatct tcttcctgca gctggccgac gcgccgctga tggcgatgga cgtgctgcag 8700 tggagccggc actaccgctg cttccccggg cagggcggct tcgatgtcgc cgggctcgtc 8760 gggcatgtga tgcgtgccgg atacgaaggg ccgctctcgc tggaggtgtt caacgacgtg 8820 ttccggcagg cggaggccgg gccgaccgct gtcgacgcgt tgcgttcgct gctcgtgctg 8880 caggagagcg tcggcctcac cgcgctgccc gctcctgccg taccgaccgg tgtcgccttc 8940 gcggaactga tcacccccga tgtcgaaccg ctgtcggaga tcctcgaagc gctcggcttc 9000 gcccgcacgg cccggcaccg cagcaagccg gtcgacctgt ggcagcaggg tgaggcccgg 9060 ctgctgctca acacgggtcc ggccgcacga cgggacggta cccagctggc cgccgtcggt 9120 ctggagtccc cggatccggc gggcgcggcc cggcgggccg aggcgctgct cgctcctgtg 9180 ctgccgcgcc ggcgcgcccc cgaggacgcc ccgctggacg cggtcgccgc acccgacggc 9240 acggaactct tcttctgcgc cacggaccga cccgaactgc ccagctggac cggcgacttc 9300 gagcccaccg cgcacaagtc gcccgtcgtg cacacgtcgc ttgtcgcgca cgagtcgccc 9360 gtcgccgggg gcatacaccg catcgaccat ctggccctca cccagccctg gcaccagttc 9420 gacgaggcgg ccctcttcca ccacagcgtc ctcggactcg gcgcgcagga gagcgtggac 9480 gtcgccgacc cgtacgggct gttgcgcagc cgtgccgtca ccaacgccga cggcagtgtg 9540 cggatcgccc tcggtgtcgg agcggccccg agcgccgacg agggcggccg ggcccagcac 9600 atcgccctcg cgaccggcga catcgtcact gcggcccgcc ggttccggga cgccggaggc 9660 cgcctcctgc ccatccccgc gaactactac gacgacctcg cggccaagta cgagttgggt 9720 gacggcgagc tggaggcgta ccgcgaactc ggcatcctct acgaccggga ggcggacggc 9780 gccttccggc actgctacac ggagacgatc ggccgcgtct tcttcgagct ggtccagcgt 9840 gacgctggct accgcggcta cggcgcgcag aacgccccgg tgcggctcgc cgcccagcac 9900 gtacggcggc cggtgcgctg actccacacc ggagggcgct ctctcaccgg attctcaacc 9960 tccgccaggc cgggcccgat cgcaggggcc cggcctgcgg catgcgctag cactgagcat 10020 tggcggtatg caagcaggcg gacattaccc acgagttgac attgaaacgt caagagtcgc 10080 cgctatcatc actccactac ggagcgaccg tccgccgccg accaccacca cggctgcacg 10140 gaatcggcca cacctcccac ttacacctgg cgcggtcggg catgcccgca cgcactcgtg 10200 caggacggaa agcagcgcat ccatgggcaa caacaggggc agagggctcc aggccaatgt 10260 cctcggcacg ttcgacacgg tcgtgatggc gatcgccggc agcgccccgg cgtactcgct 10320 cgccgcgacc accgcggttc tcgtcggcgc ggtgggtctg gccagccccg cggcgctgct 10380 gtactgcgcg atacccatgc tgggcatagc cctggccttc agctatctca gccggatcga 10440 caccaacgcg ggcgccagct actcctgggt cggccgcacg ctccacccct tcctcggctt 10500 catcagcggc tgggcgctgg tcgtctcggc caccatcttc atggtcgccg gttcgctgcc 10560 cgccggttcg atgacgctgt ccctcttcga cgaggggctc gcggacaaca cggccctgtc 10620 caccctggtc ggcgcggcct ggttcgtcgc catgctgctg gttgtgctgg gcggcgcccg 10680 gctcaccgtc cgcgcgcagc tgatcatgtc cggtgtcgag ctcgccatcc tggccctctt 10740 cgccgtcggc gccgtgctcc acaccgacaa tgcccggccc ttcgactggt cctggctcgg 10800 cttcagccac ttcgacgggg tgagcggctt cgcgtccggc gcgctcatcg ccgccttcta 10860 ctactggggc tgggacgtca cctccaacct cagcgaggag accaaggaca gccggcgcac 10920 cacgggcctc gcgggcctga tcggcgtggg catcgtcttc ctcctcttcg aggccttcac 10980 catcgcggtg aacgtcatcc tcacttcgca gcagatccag gacaacgacg ccaacgtcct 11040 cgcggtgctc ggtgaggaga tctggccggg ctggggcggc aagctgctga tcgtctccgt 11100 gatgctgtcc accatcgcca ccctggagac cacgctcatc caggtcacgc gttccctgtt 11160 cgcgatgggc cgcgaccaca cgatgcccag cgccctgggc aaggtccacc gcacgtggaa 11220 caccccgtac gtcgccatca ccgtggtcgg cgcggtcgca ctgctgatgc tcgtggcctc 11280 caacgccctc ggcagcgtcg gcgacatcct ggagaacgcc atcgccgcga tcggcctcca 11340 gatcgccgtg tactacggcc tcgcggggct cgcggtcgtc gtcgcctatc gcaagatgct 11400 gctgaagtcg ccgtccaact tcctcttcgg cgggctgtgg ccgttcctcg gcgccctgtt 11460 catgttctgg atcttcatcg agtcactgag cgatctgagc aacgcctcga tcgccatcgg 11520 catcgggggc ctggccgtcg gactcgtccc catgctctgg tactggctcc agggcagcga 11580 ctactaccgg ccggcggacc tggacgccag cgacgtcgtc gagacggact acatacccga 11640 cggctacacc gccgccgagg cggaacgttc ccgcgtccac gagggtctcc ccaccgactt 11700 ctgagagggg tcccgatggc gagagaccgc ttcaagcccg agttcgaccc cgactgcggg 11760 gacgcgcccc tcacctccgc caaacaggac atcgtcatcg gccgctggca ggggctgcgg 11820 gatctgctgc ggaccaccgg ccccgtgtgg cttgcgaggg gccaccggat gcggctgctc 11880 gcgcaggcct gcgcgagcac ctcgacggcc gagtcctggc tggccgctga gccccacagc 11940 ggtgacgcgc tggtgctgcg ggccgcgacc gagacggcac gggccttcaa cctggccatc 12000 gccgcgggcc ggggtgtccc catcgacctg aaccgcgtcg acggcgcggt catggcctgc 12060 ctccaggccg ccgaggcgta cgtcgacgac cccactccct ggatctcgct catcgccgtc 12120 gcgcggctct atcccaccgg ggtgcggcgc caggaactgg gacgctggtg ggacgagctg 12180 cacgcccgcg acccctacag cgtggagggc catctccagg tgctgcacta ctactcggcg 12240 cgctggcacg gtacccacgg cgtgatgtac gacttcgccc gggacgcggc cggggtggct 12300 ccgccggggt gtgcgctgcc ggtgctcgtg cagttcgccc gggtggagga gtaccggtac 12360 atcctggaag gggcgaaggg gcagcagggg tctctgaccg gggtcagtca gcactggaat 12420 catgacgggg ccgtcagtga tgtgcggcgt acctggcagc ggtggatcac gggccgtccc 12480 gacgcgcccg tcgagccggg ggaaatccac cagctcaacc acctcacgca cgccgcctgt 12540 cacgccgggg tgccggacat caccgcctca ctgttccgcc tcctcggccg acgggccacg 12600 cggacgccgt ggtcgtacac gggtgacccc gagaaacaaa tcacgagatg gcacaacaac 12660 ccgaagcacc gtgcgtgagc agtcgagggt agtttttcgc ccccgccgcc cctacccatt 12720 cccgtccctt acttgggagc cagcccccaa acccccgctc ctcaaacgcc ggaggggctg 12780 gggggagggg gctgctgggg gtgggtgggt tggccgtggg tgggcaatcg gcctggaggc 12840 ctcgtcctca atcgccggac gggctgggtg gtgggggctt gcggggtggg ttgtgggtgc 12900 gggttggctg ttgttggccg cgcagttccc cgcgccccta aaaacctagg ggcgcgggga 12960 actgcgcagt cttttggggg ttcgggggcg gagcccctga gtaagggacg atgggggtcc 13020 ccccgctcga gcgaagccga gagtggggga gggtaggggc ggcgggggcg aggaaactac 13080 cccggcggca aggtgttcca ctcgctgacg accgggcggt cgtgttccag ggacaggcgg 13140 ctcaacgtgc cggttccgag tcggaacagg cgcccgtccg cggcgggcag cccgagccgc 13200 cgcgccgtaa ggacacgaag gaagtgggcg tgcgccacaa gcaccacgtc accgtcgccg 13260 agcgatttct ccgcccgcgc gaggacacga tcggcccgcg caccgacctc gtcggcagac 13320 tcgcccggat gcccgtccga ccccgccggc accccgtcgt tccacaagta ccagtcggga 13380 cgagtgcggt ggatgtcgac cgtggcgata ccttcgtacg ccccgtagtc ccactcgtgc 13440 atgtccggat cggacaccgc cccggtcagc cccgccagct ccgcggtccg tatcgcccgc 13500 ccgagcggac tggagagtac gagcgcgaag tgccgcccgg cgaacagcgg gacgagggac 13560 ttggcctgtt cctcaccgcg ctgggtgagg ggcagatcgg tccagctggt gtgccggccc 13620 gacctgctcc actcggtctc gccgtgccgg acgagaagga ggtcgcccat gactactgac 13680 tcctacttcg cggactcgac ggcgtggccg ccgaactggt tgcgcagcgc cgcgaccatc 13740 ttcatctgcg gcgagtcgtc ctgccgggac gagaaccgcg cgaagaggga ggccgtgatg 13800 gcgggcagcg gcacggcgtt gtcgatggcc gcctcgacgg tccagcggcc ctcgccggag 13860 tcctccgcgt aaccgcggag ctgctgcagg tgctcgtcct cgtccagcgc gttgaccgcc 13920 aggtcgagca gccaggaacg gatgaccgtg ccctcctgcc aggagcggaa cacctcgcgc 13980 acatccgtga cggagtgcac cgcttcgagg agctcccagc cctcggcata ggcctgcatc 14040 atggcgtact cgatgccgtt gtggaccatc ttcgagaagt ggccggcgcc caccttgccc 14100 gcgtggacga acccggcgtc gccctccggc ttgagggcgt cgaagatcgg ctggaccttg 14160 gcgatgtgct cggcgtcgcc gccgaccatc agggcgtagc cgttctgcag gccccagaca 14220 ccgcccgaga caccggcgtc gacgaagccg atgcccttgg ccgccagctc ctcggcgtgc 14280 ttctcgtcgt ccgtccagcg ggagttgccg ccgtcgacga cggtgtcgcc cggctccagc 14340 aggtcgccga gttcgtcgat gacaccctgg gtggcggccc cggccgggac catcacccag 14400 accacgcgcg gtccttcgag cttgccgacc agttcggtca ggctgctgac gtcggagagg 14460 tccgggttgc ggtcgtagcc gatcacggtg tggcccgcgc ggcggatccg ctcgcgcatg 14520 ttgccgccca tcttgccgag gccgatgagc ccgagctgca tcgcagtcat gtcagttcac 14580 ttctttcgca gagtgcggta cgtggccacg agggcggcgg tggagggatc gagaccgggg 14640 acgtcggcgc cctcggtgag ggcgggttcg acgcgcttgg cgaggacctt gccgagctcg 14700 acgccccact ggtcgaagga gtcgatgttc cagatcgcgc cctgcacgaa caccttgtgc 14760 tcgtagaggg cgatgagctg gccgagcacc gacggggtca actcggcggc caggaccgtg 14820 gtcgtggggt ggttgccgcg gaaggtgcgg tgcgggacct gctcctcggg caccccctcg 14880 gcgcgtacct cgtcggcggt cttgccgaac gcgagggcct ggccctgggc gaagaggttc 14940 gccatcaaca ggtcgtgctg ggcggcgagt tcggcaccca gctcgtcgac cggttgacga 15000 agccgatcag atcggccggg atcgtcttcg tgccctggtg ggagcaactg gtagtacgcg 15060 tgctggccgt tggtgccggg cgtgccccac acgacgggcc cggtctgcca ctcgaccggg 15120 tgtccctcgc ggtcgacgga cttgccgttg gactccatgt cgagctgctg caagtacgcg 15180 gtgaacttgg acaggtagtg cgagtacggc agcaccgcgt gcgactgggc gccgaagaag 15240 tcgccgtacc agacgcccaa caggccgagc agcaaaggcg cgttggactc ggcgggcgcg 15300 gtcctgaagt gctcgtcgac gaggtggaag ccgtcgagca tctcccggaa gcggtccggg 15360 ccgatcgcga tcatcagcga cagaccgatc gccgagtcga acgagtaacg gccgccgacc 15420 cagtcccaga actcgaacat gttggccgtg tcgataccga agtccgcgac cttctcgacg 15480 ttcgtcgaca gcgccacgaa gtgccgggcc acggcctcgg actcaccgcc gagaccggcg 15540 agcagccagg tccgcgccga ggtcgcattg gtgatcgtct cgatggtcgt gaacgtcttc 15600 gaggcgacga cgaacagcgt ctccgccggg tccaagtcgc ggacggcctc gtgcaggtcg 15660 gccccgtcca cgttggacac gaagcggacc gtcagatcgc ggtcggtgaa ggcgcgcagg 15720 gcctcgtacg ccatcgccgg accgaggtcg gagccgccga tgccgatgtt gacgacattg 15780 cggatgcgcc tgcccgtgtg gccggtccac ttgccggagc ggacctgctc ggcgaagtcc 15840 gacatcttgt cgagcacggc gtgcaccttc ggcacgacgt tctcgccggc gacctcgacg 15900 accgcttcga gcggggcgcg cagcgcggtg tgcagcacgg cccgcccctc ggtgacgttg 15960 atccgctcgc cgcggaacat cgcgtcccgc agcccgaaca cgtcggtggc ggtggcgagt 16020 tcctggagca gcgcgagcgt ctcgtcggtg atcaggtgct tggagtagtc gatgcgcagg 16080 tcaccgaccc gcacggtgta gcgcgaggcg cgctccgggt ccgcggcgaa cagctcgcgc 16140 agctgcggat ggaggagcgg gcccgcgcgg tggtcctcca gggccgtcca ctcggggcga 16200 cgggtgagcc tgggcgtccg gggctcggac ggaggcgtgt caggcacggc tctcctcctc 16260 tcccgagtcg tcaccgcgca gggcgacggc gtacatctcg tccgcgtcca ggcgccgcag 16320 ctcctcggca atcagttcgg aggtgctgcg gaccttcagc gcgagggtgc gcgagggctg 16380 gcccggcagc gacagcgtcg cgaggggacc ctcgggacgg tcgatgacga tctcgccgtt 16440 cttggtgccg agccggacgg cggtgacgac cggccccgcg gtcacgaccc gctcgaccgc 16500 gacctggagg cgggcctcca gccagcgggc cagcagttcg gcgctcgggt tctcggcctc 16560 gctctccacg gcggccgagg tgatcttcgt acgggcctgg tccagggcgg cggcgagcat 16620 ggaacgccac ggagtcagcc gggtccaggc caggtcggtg tcgcccggcg cgtacgaggc 16680 ggcacgggcc tccagggccg cgagcggtgc ctcgaccgcg tacatgtcgg tgatccggcg 16740 ctgtgcgagg gcgcccagcg ggtccttggc cgggacctcg ggcgcgtcca ccggccacca 16800 gacgacgacc ggcgcgtccg gcagcagcag cggcaggacc accgagtcgg cgtgctcgga 16860 cacctccccg tacagccgca gcacgaccgt ctcgccggtg cccgcgtcgg cgcccacccg 16920 gacgtcggcg tcgaggtgcg agttcgtgcg gtcgcgcggg gtgcgtgcgt gccgcttgat 16980 gacgaccagg gtgcgcgagg ggtgctcgtg cgaggcctcc tcggccgcct tgatcgagtc 17040 gtaggcgttc tcctcgtccg tgacgatgac catcgtcagg accatgccca cggcgggcgt 17100 gccgatggcg cggcggccct ccactagggc cttgttgatc ttgcttgccg tggtgtcggt 17160 caggtcgatc ttcatggcct gcgccagctc cgtccgtctc gtgcgagcat ctcgtcggct 17220 tccccgggac cccagctgcc cgaggcgtac tgcgcgggcc tgccgtgctt gtcccagaac 17280 tcctcgatcg gatcgaggat cttccaggac tcttccacct cctggtgtcg ggggaagaga 17340 ttggcgtcgc cgagcaggac atccaggatc agccgttcgt acgcctccgg gctggactcc 17400 gtgaacgact cgccgtaggc gaagtccatg gagacgtccc ggatctccat cgacgtaccc 17460 ggcaccttcg atccgaagcg caccgtcatg ccctcgtcgg gctggacacg gaagacgatc 17520 gcgttggcgc cgagctcctc cgtggcggtc gagtcgaacg gggagtgcgg ggcgcgctgg 17580 aagacgaccg cgatctcggt gacccgccgg ccgagccgct tgccggtccg caggtagaag 17640 gggacgcccg cccagcggcg gttgtcgacc tccagcttga cggccgcgta cgtgtcggtc 17700 ttcgacgcgg ggtcgatgcc ctcctcttcg aggtagccgg gcaccttggc gccgccctgc 17760 cagccctccg cgtactgccc gcgcacggtg tgctcgccca ggtcctccgg cagccgcacc 17820 gacttgagga ccttgagctt ctcggtgagc agcgagtccg cgtcgaaggc gatgggctcc 17880 tccatcgcgg tcagcgccat cagctggagc aggtggttct ggatgacgtc acgggccgag 17940 ccgatgccgt cgtagtaccc ggcccggccg ccgatgccga tgtcctccgc catggtgatc 18000 tgaacgtggt cgacgtacga ccggttccag acgggctcgt acatctggtt ggcgaagcgg 18060 agcgccagga tgttctggac ggtctccttg ccgaggtagt ggtcgatccg gaagacctgc 18120 tccggctcga acacgtcgtg caggaccttg ttcagctcac gggcgctctt gaggttgtga 18180 ccgaacggct tctcgatcac cgcgcgccgc caggaacccg ccggcgcgtc ggccagcccg 18240 tgcttcttga gctgcttgac gaccttcggg aagaacttcg gcggtacgga gaggtagaag 18300 gcgtagttgc cgctggtgcc ccgggaggcg tccaactcct cgacggccgc gcgcagttgc 18360 ttgaacgcct ggtcgtcacc gaagtcgccg gggatgaacc gcatgccctc ggcgagctgc 18420 tgccagacct cctcgcggaa ctccgtacgg gcgtgctcgc gcaccgagtc gtggacgatc 18480 tgggcgaagt cctcgtcctc ccagtcgcga cgggcgaaac cgacgaggga gaagcccggc 18540 gggagcagtc cgcggttggc caggtcgtag acggccggca tcagcttctt gcgggccagg 18600 tcgcccgtga caccgaagat gaccagaccg gacggcccgg cgatcttcgg cagccggcgg 18660 tcgccggggt gacgcagggg gttgtcccag tcggacgcga ggtccagcgc ctgggcgagg 18720 gactcgggcc cgaggtcggc ggcggtggcg tccggcgtcg taccggccgt ttccgctgcg 18780 ggggtcccgg tctccgctgc cgttccggat gccttcgccg gggccttctt gggacgcttg 18840 tcgctcattc tccgtcaact cccttgcggc tcagtgagac ggcgaccgcg tcgagcagtt 18900 cgccccaggc ggcctcgaac ttggcgacgc cctcgtcctc cagctgccgt acgacctcgt 18960 cgtacgagat gccctgcttc tcgacggcct ccaggtcggc gcgggcctgg gcgtagccgc 19020 cggtcaccgt gtcgccgcgg atctcgccgt ggtcggcggt tgcgtcgagc gtggcctccg 19080 gcatggtgtt gaccgtgccg ggcgcgacca gctcgtcgac gtacagcgtg tccttgtagg 19140 acgggtcctt gacgccggtc gaggcccaca gcggacgctg cttgttggcg ctcccgccga 19200 gggcggtcca gcggtccgag gagaagacat cctcgtacgc ctcgtacgcg agccgcgcgt 19260 tggcgagcgc cgccctgccc ttgagcgcga gggcctcgtc cgtgcccacc ttcgccagcc 19320 gcttgtcgat ctcgctgtcg acgcgggaga cgaagaagga ggccacggag tggatcgtgg 19380 agaggtcgag gcccgcggcc cgtgccttct ccagaccggc caggtaggcg tccatcacct 19440 cgcggtagcg ctccagcgag aagatcagcg tgacgttgac gctgatgccg cgcccgatga 19500 cctcggtgat cgcggggaga ccggccttcg tcgccgggat cttgatcatc acgttagggc 19560 ggtcgaccag ccaggcgagc tgcttggcct cggcgatggt cgccgtcgtg tcgtgggcga 19620 ggcgcgggtc gacctcgatg gagacccggc cgtcccggcc gccggtcgcg tcgtacaccg 19680 gacgcaggat gtcggcggcg gcgcggacgt cggcggtggt catcatccgt acggcctcgt 19740 cgaccgtcac gccgcgtgtg gcgaggtcgg ccagctgctc ctcgtaaccc tcgcccgagc 19800 cgatcgcggc ctggaagatg gaggggttgg tcgtgacgcc gacgacgtgc ttcgtctcga 19860 tgagctcggc gaggttgccg gacgtgatcc gcttgcgcga gaggtcgtcc agccagacgg 19920 agacgccttc ggcggagagg ttcttgaggg tttccgctgt tgtggttgct tcggtggtcg 19980 cggttgcttc ggtcacagtg atcatcttcc ttcgggcgat cggatcaacc gcggactgcg 20040 gcaagagatt cccgcgcggc ggaggccacg ttctcggggg tgaagccgaa ctcggcgaac 20100 agggtcttgg cgtcggcgga ggcgccgaag tgttcgaggg agacgatgcg tccgtggtca 20160 ccgacgtacc ggtaccaggt caggccgatg cccgcctcga ccgccacgcg ggccttcacg 20220 gacggcggaa gcacgctcgc tcggtactcc ggcgactgct cctcgaacca ctcgacggac 20280 ggcatcgaca ccacacgcgt tccgacgccc tcggcctcca actgctcgcg cgcggtcacg 20340 gcgagctgca cctcggagcc ggtcgcgacg aggatcacgt ccggggtcgc ggtggaggag 20400 tcgcgcagga cgtaaccgcc cttcgccgcc tcggggttgg cctcgtacgt cggcacgccc 20460 tgacgggtga gggcgaggcc gtgcggggcc gggttggtgg cgtgccgctt gaggatctcg 20520 gcccaggcga tcgacgtctc gttggcgtcg gcggggcgca cgatgttcag gcccgggatg 20580 gcgcggagtg aggccaggtg ctcgaccggc tggtgggtcg ggccgtcctc gccgaggccg 20640 atggagtcgt gcgtccagac gtacgtcacc ggcagctgca tcagggccga aaggcggacg 20700 gcgttgcgca tgtagtcgga gaagacgagg aacgtgccgc cgtagatccg ggtgttgccg 20760 tgcagcgcga tgccgttcat ctccgcggcc atcgagtgct cacggattcc gaagtggacg 20820 gttcggccgt acgggtcggc ctccggcagc gggttgccct tcgggaggaa ggacgaggtc 20880 ttgtcgatgg tcgtgttgtt cgagccggcg aggtcggcgg agccgcccca cagctcgggg 20940 agtacgtcgc cgagcgcctg gaggaccttg ccggacgcgg cacgggtcgc gacggacgtg 21000 cccgcctcga acgccgggag cgcggactcc cagccctcgg ggagctgacc ggccacgatc 21060 cggtcgaaga gcttggcgcg cgcgggctgc gcggtgcgcc actcggccag ctgcttgttc 21120 caggcggcgt gcgcctcggc accccggtcg agggcccgcc gggcgtgggc gagaacctcg 21180 tcggcgacct cgaaggtctg ctccgggtcg aagcccagga ggcgcttggt ggccgcgatc 21240 tcctcggcgc cgagcgccga gccgtgcgcg gcctcggtgt tctgggcgtt gggcgcgggc 21300 caggcgatga tcgtgcgcat cgcgatgatc gaggggcggc cggtctcggc cttcgccgcg 21360 gtcagggcct cgtggagcgc gcgcgggtcg atgtcgccgc cgagcgcggg ctcgatccgc 21420 tgcacgtgcc agccgtacgc ctcgtaccgc ttcagtacgt cctcggagaa cgcggtggcg 21480 gtgtcgccct cgatggagat gtggttgtcg tcgtagacga agacgaggtt gccgagcttc 21540 tggtggccgg cgagcgagga cgcctcggcg gagatgccct cctccaggtc accgtccgag 21600 acgattgccc agacggtgtg gtcgaagggg gactcgccct cgggagcctc ggggtcgaac 21660 aggccgcgct cgaagcgggc ggccatcgcc atgcccaccg cgttggcgac gccctggccg 21720 agggggcccg tggtcgtctc gaccccggcc gtgtgcccgt actccgggtg tccgggcgtc 21780 ttcgagccgt gcgtgcggaa ggccttgagg tcgtcgagct ccagctcgta cccggagagg 21840 aagagctggg tgtagagggt cagcgaggtg tggccggggg agaggacgaa gcggtcgcgg 21900 ccggtccact cgggatccgc gggatcgtgt cgcatcacct tctgaaagag ggtgtacgcc 21960 gccggtgcga ggctcatcgc ggtgcccggg tggccgttgc cgaccttctg cacggcgtcc 22020 gccgcaagca gacgggccgt gtcgacggct cgccggtcca gatcggtcca ctcaaagctg 22080 tcaggtgtct gcgtgctcat cttcaagaag tcctcggtag aagcccagta actgctcggt 22140 tgcgttcaaa cttaaaagtc agacttttgc cggggaaggt ggccctgcgt cagcctttgg 22200 tgaaagtggg acaaggactg cgggaccgag tcgacgcgag agggacatgg cggacataag 22260 cacccaagac gccgacgccg gcgacgggat caggaccttt ccctttcccg tcgacctgag 22320 cgtctgcgga gtcggcatgc aggtcggtcc gatggagccc ggccggacct ggcacgcgaa 22380 cgaggcgatc gagcgcgtcc accgcatcga cttccacgtg gtcatgctct tcggggacgg 22440 cccggtccgg cacatggtcg acttcaccga gtacgaggtc tccgccggtg agctcctgtg 22500 gatacgcccc ggacaggtcc accgtttctc cagcacggag gcctaccgcg gaacggtcct 22560 gatcatgcag ccgggcttcc tgccccgcgc cacggtcgag tcgaccggcc tctaccgcta 22620 cgacctgccg cccctgctca ggcccgaccc ggcccagctc gccggactga gggccgccct 22680 cgcccagctg gaacgcgagt acgtcgacac gaccacgctg ccgctcagcc tgcacacctc 22740 cgtgctgcgg cactccctga cggcgttcct gttgcgcttg gcgcacctcg cggccagctc 22800 cgccgacgcg gcacgcgacc aggaccaggc ggacaccacc ttcacccgat tccgcgaggc 22860 cgtcgagaag ggcttcgcca ccaaccacag cgtcagcgcg tacgccgacg ccctcggcta 22920 ctcccgccgc accctcgtcc gggcggtccg cgccgcgacg ggcgagaccc cgaagggctt 22980 catcgacaag cgggtcgtcc tggaggccaa gcggctcctc gcccacacgg acatgccgat 23040 cggccgcatc ggggtctcgg tgggcttccc cgacgcggcc aacttcacca agttcttcca 23100 ccagcacacc ggggtggccg ccggcggcgt tcagggctga actgcactga gaaatcagag 23160 ggattggacg aatccgagga atcgaaggaa ggagggggcg ggcctgtccg gcacccctcc 23220 tcaccgcact tggccctcag cgggcgaagc tgaaccagtt cacgttcacg aagtccgccg 23280 gttggccgct cgtgaaggtc agatagacgt cgtgcgttcc ggtgacgccg ctggtgttcg 23340 tcgggacggt ccgccagctc tgccagccgc cggtgttggc gagcgcgaag gtgccgatcg 23400 gtgcgttcgt acggctgtcg aggcggactt cgaccaggcc actggcgccg ccccccacac 23460 cgctcgcgac ccgggcgctg aactgcctgg ccgccgtgga gccgaagttg acgcccttgt 23520 actgcaccca gtcgccgttg gcgagggcgc cgatgttctg gccgccgcca gagtcggtcg 23580 tggtctcggt gatagtgccg ctctgaccgt cgaacgactc ggcctggatc gcgctgtacg 23640 cgtcacggtt gccggtcggc ggaggtgtgg tgccgcccgc ggactgcagc acctggacgt 23700 agtcgacgac gagtgggttg ccgggcacgg tggcaccgtc cggaccgccg ccgaaggcgt 23760 ccacgaaacc gccgcccatc gccacgttca ggatgatgaa gaagccatgg ttcgtggcgt 23820 tcgcccaggt cgtggcgtcg acctgattcg cccgtacggt gtggtagttg atgccgtcga 23880 cgtagaagcg gatctcctcc gggctcgtcg accggtccca ctccatgccg tacgtgtgga 23940 agccggcctg gcaggtcgcg cccgggcagg cggtcgagcc gccgaggccg gtcgtctcgt 24000 tgcaggggcc gcccgggctg gagccgcaat gcatcgtggc ccacacggtg ttgagaccct 24060 ggacgttctc caggatgtcc aactcgccga cgctgggcca gttctggtag ttgccgcggt 24120 agggcgcgcc cagcatccag aacgccggcc agtagccgcg ggcggcggcg cccgtcacgt 24180 tcggcatctg gatccgggac tggacacgca gtttgccacc ggcgggcggc tggaagtcgg 24240 tgcggttggt ctcgatgcgg cccgaggtcc agttgcccgc ggcgtcgcgg cgcggggtga 24300 tccggaggtt cccggcgccg tccagcgcca cgttgttggt gctggacgtc atgttctcga 24360 tctcgcccgt gccccagttg gagggcccgc ccgggtagcc gtggccggtc gcgtactgcc 24420 agttcgaggt gttgacgccg gtgcccgccg tgccgttgaa gtcgtcgacg aagacctggg 24480 tccagccgct gggcggcgcg ggcgcgtccg cctctgccgc cgatgccccg gccgccgcca 24540 ggctgagcac gctgacgagc gcgatgagcg tgcgccggag tggaccgcgt ctgacgggtg 24600 tgcctgaagt ttccctcatg ggtgcctctt cgggtacgga gtgggggtgc gcgggtggtt 24660 cgggggtggt cccgaggtgg ttcggggtgg ttcgggggtg gtccggggtg gttcgtgggt 24720 acgcgagagc atttgagagc gctctcaacg cgaccgcgcg gccaatgtgc tcttcggcac 24780 tccggtcgtc aagagataaa gcagagaaag ctcttggggg gcaggggagt tcacaccgtg 24840 aaggggcgga tcggacgcgg cgtgagagag cgctccgtgc cgttcagttc gtccacgacg 24900 ggttccagcc gttgaggccg tcggccggac gcacaaaagc cccccggtac cgcggctcag 24960 gccgcggtac cggggggcgt cgaagggtgt tctacgaaga gcaggacgtc acgctcagcg 25020 cgagccgagt acggtccagg cgtcgccgcc ggcgagcagc gcggagaggt cgcccttgcc 25080 gttctgttcg atggccatgt cgagttggtc cgacatctgg acgtcgtaga cgggccggtc 25140 gacggagcgg aacacaccga tgggggtgtg gtgcagggtg tccgggtcgg cgagtcgtga 25200 gagcgcgaac gcggtggtgg gggaggcgga gtgtgcgtcg tggacgagga tctgcggctc 25260 gttctccggg gtgacggtga cggccttgag gtcgccggtc cgctcatcgc gtacgacacc 25320 gcggttgccg tcggcgccga agcggatggg cttcccgtgc tccaggcgga tcaccgcctc 25380 ctcggcctgc tgcctgtcct tcagcacctc gaaggcgccg tcgttgaaga tgttgcagtt 25440 ctggtagatc tcgacgaggg cggtgcccgg gtgggccgcg gcctgccgca gcacctccgt 25500 cagatgcttg cggtcggagt cgacggtccg cgccacgaat gacgcttccg cgccgatcgc 25560 gagcgacacc gggttgaagg gtgcgtccag ggagcccatc ggggtcgact tggtgatctt 25620 gccgacctcg gaggtggggg agtactggcc tttggtgagg ccgtagatcc ggttgttgaa 25680 gagcaggatc ttgaggttca cgttgcggcg cagggcgtgg atgaggtggt tgccgccgat 25740 ggacagcgcg tcgccgtccc cggtcaccac ccacacgctc aggtcgcggc gggaggaggc 25800 gagtccggtg gcgatggcgg gggcgcggcc gtggatggag tgcatcccgt aggtgttcat 25860 gtagtacggg aagcgggagg agcagccgat gcccgagacg aagacgatgt tctccttggc 25920 gaggcccagt tcgggcatga agccctggac ggccgcgagg atcgcgtagt cgccgcagcc 25980 ggggcaccag cgcacttcct ggtcggactt gaagtccttc atggactgcc tggcctcggc 26040 cttgggcacc agggtgagcg cctcgatcgt gcccgggcct tccgtggtcg tctcagccat 26100 cgatggcctc cttgagagcc gtggcgagtt gttcggcctt gaacggcatt ccgttgacct 26160 ggttgtagga gtgggcgtcc accaggtatt tcgccctgac cagggtggcg agctggccga 26220 ggttcatctc ggggatcacc accttgtcgt aacgcttcag caccgtgccc agattcgccg 26280 ggaacgggtt gaggtgccgt agatgcgcct gcgcgatcgc ttccccggcc gcgcgcagcc 26340 gccgcacggc cgcggtgatg ggtccgtagg tggagcccca gcccaggaca agggtgctgg 26400 cgccgtgcgg gtcgtcgact tccacatccg gtacgtcgat gccgtcgatc ttggcctggc 26460 gggtgcggac catgaagtcg tggttggcgg ggtcgtagga gatgttgccc gagccgtcct 26520 gcttctcgat gccgccgatc cggtgttcga gaccgggtgt tcccgggatg gcccacgggc 26580 gggcgagggt ctgggggtcg cgtttgtagg gccagaagac ttcggtgccg tcgtccaggg 26640 tgtggttggg gccctgggcg aactgcacgc gcaggtcggg gagttcgtcg gtgtccggga 26700 tccgccacgg ttcggatccg ttggccagat agccgtcgct cagaaggaac accggggtgc 26760 gataggtgac tgcgatacgg gcggcctcga tcgcggcgtc gaagcagtcg gcgggggtcc 26820 ggggggcgac gatcggcacc ggtgcctcgc cgttgcggcc gaacatggcc tgcagcagat 26880 cggcctgttc cgtcttcgtc ggcagaccgg tggaggggcc gccgcgctgg atgtcgatga 26940 ccagcagcgg cagttccagc gacaccgcga gcccgatcgt ctcgctcttg agcgcgacac 27000 ccggtccgga ggtggtggtg acggcgagcg agccgccgaa ggcggcgccc agtgcggcgc 27060 cgatgccggc gatctcgtcc tcggcctgga aggtccggac gccgaagttc ttgtgcctgg 27120 acagttcgtg caggatgtcg gaggccgggg tgatcggata cgagcccagg tagaggggca 27180 ggtccgcctg ctgggaggcg gcgatcaggc cgtaggacag ggccaggttc ccggagatgt 27240 tgcggtaggt gccggtcggg aaggccgcgg tggccggcgc gatctcatag gagacggcga 27300 agtcctcggt ggtctcgccg aagttccagc ccgcgcggaa cgcggcgatg ttggccgccg 27360 cgatatcggg cttcttggcg aacttggtgg tgaggaactt ctcggtgccc tcggtcggcc 27420 ggtggtacat ccagctcagc agccccagcg cgaacatgtt cttgctgcgc tcggcctcct 27480 tgcgggtgag gtcgaactcc ttgagggcct cgacggtcag cgtggtgagg gggacggggt 27540 ggacgctgta gccgtccagc gaaccgtcct gaagggggct cgtggcgtag ccgaccttct 27600 gcatcgcccg tttggtgaac tcgtccgtgt tgacgatgat ctccgcgccg cgcggtacgt 27660 cggcgatgtt cgccttcagg gcggccgggt tcatcgccac gaggacgttg ggcgcgtcgc 27720 cgggggtgag gatgtcgtgg tcggcgaaat gcagctggaa agacgaaacg cccggcaggg 27780 tgcctgcggg cgcgcggatc tcggcgggga agttcggcag tgtcgaaagg tcgtttccga 27840 aggatgccgt ctcggaggtg aagcggtccc cggtgagctg cataccgtca ccggagtccc 27900 ccgcgaacct gatgatcacc cggtccagcc ggcgtacatc cttcgacggc aggggggcgg 27960 aatcatcaag ggcgcctgaa tgattcggga ggtcgggcct ggtgaacacg gagtctccct 28020 aggaattgat ttcgcagatg agggcgccgg aggtgacgga ggcgccgact tcggcggaca 28080 gggccttgac ggtgcccgag cggtgggcgt tgaggggctg ctccatcttc atggcctcca 28140 ggacgacgac gaggtcgcct tccttgacct cctggccctc ttcgacggcg accttgacga 28200 tggtgccctg catgggggag gcgagggtgt cgccggaggc ggcggggccg gacttcttgg 28260 ccgcgcggcg tttgggcttg gcgccggctg cgaggccggt gcgggccagc gacatgccca 28320 gggagacggg cagggagact tccaggcgtt tgccgccgac ctcgacgacg acggtctcgc 28380 ggcccggctc gtcttcgacc tcggtgtccg cgggagcggc gaagggcttg atgtcgttga 28440 cgaactcggt ctcgatccag cgggtgtgga ccgtgaacgg gtcggtggag ccggtcagtt 28500 cggggcgaag ggcggggtcc ttgacgaccg cgcggtggaa ggggatcgcg gtcgccatgc 28560 cctcgacgtt gaactcggcg agcgcgcggg cggcccgttg cagggcctgc tcgcgggtgg 28620 cgccggtgac gatgagcttg gcgaggaggg agtcccaggc cgggccgatg acgctgccgc 28680 tctccacgcc cgcgtccagc cgcacgcccg ggccggacgg cggggcgaag gtggtcaccg 28740 tgccgggagc gggcaggaag ccgcggcccg ggtcctcgcc gttgatgcgg aactcgaagg 28800 agtggccgcg cagttccggg tcgccgtagc cgagctcctc accgtcggcg atgcggaaca 28860 tctcgcggac gaggtcgatg ccggcgacct cctcggtcac cgggtgctcc acctgcaggc 28920 gggtgttgac ctccaggaag gagatcgtgc cgtccgtgcc gaccaggaac tccacggtcc 28980 cggcgccgac atagccggcc tccttgagga tggccttcga agaggagtac agctcggcga 29040 cctgctcgtc ggagaggaac ggcgcgggcg cctcctcgac cagcttctgg tggcggcgct 29100 gcagtgagca gtcgcgggtg gagacgacga ccacgttgcc gtgggagtcg gccaggcact 29160 gggtctccac gtgccggggc ttgtcgaggt agcgctcgac gaagcactca ccgcggccga 29220 aggcggccac cgcctcgcgg acggccgagt cgtacagctc ggggacttct tccagggtgc 29280 gggcgacctt cagaccgcgg ccaccgccac cgaaggcggc cttgatggcg atcggcagcc 29340 cgtgctcctc cgcgaaggcg acgacctcgt ccgagcccga gaccgggtcg ggggtgccgg 29400 cgaccagcgg ggcgccggcg cgctgggcga tatgggcctt gtcacccagg tcgcgaatcg 29460 cctgcggcgg cgggccgatc cagatcagct gggcgtccag gacggcctgg gcgaagtcgg 29520 cgttctcgga aaggaagccg taaccggggt ggatggcgtc cgcgccggag tccttggcgg 29580 cctgcagcac cttggccatg tcgaggtagc tggtcgcggg ggtgtcaccg cccagcgcga 29640 acgcctcgtc cgcggcccgc acatgcagtg cgtcccggtc cggctcggcg tacaccgcca 29700 cgctcgcgat accggcgtct cggcacgccc gggcgacacg aacagcgatt tcgccacggt 29760 tggcgatcaa caccttgcgt atgcgggcct gacggaggcc taggagggcg gcgctcatgt 29820 acgccaactc tgtggcgcgc cgtacccgga ggtatggccc atacgctgcc atccggctcg 29880 ccgcgatgcc gcacgggcac cgtcgccgtc cggggcggga cgtcgcgcac tgccggcgag 29940 gaggacggct gtcagggcgg cgagctcctc cgcgccggcc tctcccttca acacctggat 30000 ggacggaacg ctggtggtca cgaactctcc tgtcactgtg gggggttgcc gtgtttgcgg 30060 gagggcagtt ccgcgtgctt ggtgcgcagc atcgccaggg agcggatgag taccgtacgg 30120 gtctcggcgg ggtcgatgac gtcgtcgacc aggccgcgct cggccgcgta gtacggatgc 30180 atgagctcgg ccttgtactc cttgaccagc ctggcgcgca tcgcctcggg gtcgtcggcg 30240 gcggcgatct gcctgcggaa gacaacgttg gcggcgcctt ccgcacccat caccgcgatc 30300 tcgttcgtcg gccaggcgta ggtcaggtcg gcgccgatgg actgcgagtc catgacgatg 30360 tacgcgccgc cgtaggcctt cctgaggatc agtgagatcc gcggcacggt cgcgttgcag 30420 taggcgtaga gcagtttcgc gccgtgccgg atgacgccgc cgtgctcctg gtccacgccc 30480 ggcagaaagc ccggcacgtc gagcagcgtc accagcggga tgctgaacgc gtcgcacagt 30540 tgcacgaagc gggccgcctt ctcgctggcc gcgatgtcga ggacgccggc catggactgc 30600 ggctggttgg cgacgatgcc caccacctgg ccgtcgagcc gggcaagtgc gcagatgatg 30660 ttgcgggccc agcgctcgtg gacctccagg tagtccccgt cgtcgacgag ttccccgatc 30720 accttcgtca tgtcgtacgg ccggttgccg tccacgggga ccaggtcaag cacggcgtcg 30780 gtgcgccggt ccgcccggtc gtcggggtcc acccggggcg ggacgtcgcg gttgttctgc 30840 ggcagcatcg agagcagata ccgcacctcg gcgatgcagg tctcctcgtc gtcgtacgcg 30900 aagtgcgcga cgccgctcgt ctccgcgtgg acgtccgcgc cgcccaggcc gttctgggtg 30960 atctcctcgc cggtgaccgc cttgacgacg tcaggtcctg tgatgaacat ctgcgaggtc 31020 tcgcggacca tgaacacgaa gtccgtcagg gccggggagt acgcggcgcc gccggcgcac 31080 gggcccagca tcacgctgat ctgcgggatg acgcccgagg cgcgggtgtt gcgctggaag 31140 atgccgccgt agccggcgag cgcggacacg ccctcctgga tgcgggcgcc cgcaccgtcg 31200 ttcagggaca ccaggggtgc gccggccgtg atggccatgt ccatgatctt gtggatcttc 31260 gtggcgtggg cctcgcccag cgagccgccg aaaatgcgga agtcatgggc gtacacgaag 31320 accgtgcgcc cgtgcacggt gccccagccg gtgatcacgc cgtccgtgta cgggcggttc 31380 gcctccaggc cgaagccggt cgcccggtgc cggcgcagcg gctccacctc gctgaacgtg 31440 ccttcgtcca tgaggagttg gatgcgttca cgggctgtca gcttgccgcg ggcgtgctgg 31500 gccgaggtcg cgcgttcgcc gggaccctcg tgcacctgcc tcttgagcct ggccagctcc 31560 tcgacgtgcg tggccgtcgg ccgccgctcc tccatgatgg tcatacgtgt gccctcccga 31620 tcagtgactg gtcagggacc ggagatcccc gggcagttca cgcacgacgg tgtgcgtggg 31680 cggtgtgccg tggagcgcga cggccgccgc gtggcccgcc gggcacggca cgtcccgtac 31740 ggtccagccg tccggccctc gcggggcttc cgggccgccg gcgccgacgt acacctcgga 31800 catccagcgg ccgattccgg cgcccgtgcc tttcaggtat gcctccttgc gcgcccagac 31860 gcgggcgaag cccgcctgcc ggtccgctcc ctccaggcgt cgcagttccg cctgctcgta 31920 cggatgcagg gagggcaggc agacctccac ccgctcgggt gtcggcagcc gctccacgtc 31980 gacgccgacc gggcgggcgg ccacggcgat gagcgccgtg ccatggctgt gcgagagcga 32040 gaagtgcggg acggagccgg gcgcggcgag tacgggcctg ccgtgcgggc ccccgcaccg 32100 ggggcagtcc gcacgggcga aggcgagctt gcgcgcgggg atcccgacgt acgtggcgag 32160 cacccgcctc agcgcgatgt gcgccgccac gtacagatcc cggtcggccg ggcgccggaa 32220 ggcggcggcc cgccgccgct cggcctcgtc gagctcgtcg agttggtcga gggcgccggg 32280 tccgtacgag gggaacgcgg caccgtcctc ctcaggccga cggctcggag acagcagaaa 32340 gacttccaac gtgcttgtcc acacgtgttg ttgacgttcc atcacccgat gctcccgctc 32400 gtcgcacgtc actcagcagc accgcctgcc gtagcgtgtc ggcgctcggc gaggtgcccg 32460 cccgcaccgc cgacatgaag gccctgacgg tggccgcgac ctggtcggcg ggccggagcc 32520 ggatctcctc gatgtccgcg ccacgctgca cggtgatcac ggggatgtgg tcggccggcg 32580 gggtgaaggc gcggtcgacg gtgatccggc cctcgctgcc ccacagttcg tacccggagc 32640 ggtatgcgtg ctccatgccg aaggtgatct gcgcggtgac tccgccgggt gtgcgcagca 32700 gcgcggcgcc ggacgtctcg acgaggcgcc ccgcgccgtt cgcgagatgg gcaccgacga 32760 cctcgacgtc cggcccgagg aagtgcaggg cggcccgcag cggatagagc cccacgtccg 32820 ccagtgcgcc gccgccgagc tcggggacgt accggatgtc gtcgtcgggg agccggggga 32880 tggtgaacgc cgcctggaag gtgcggagtt caccgatcct gccgtccgcg acgaggcgcc 32940 gcaccgcctc gtgcacggga tggtggacga acatcacgtt ctccatcagc gcgagccccc 33000 gggcggcggc gaggcacagc agccgttcgg tggtcgcgga atccgtgctg aggggcttct 33060 cggcgagtac gtgcaggccc gcgtcgagcg ccgcctccac ccacctggcg tgcagcgccg 33120 cgggcagtgg cacgtacacg gcctggatgt tgtcgcgtcg caggagctcg gcgtacccct 33180 ggaccgggtg gcagtcgaag cgttcggcga cccgttcggc cttggcgagg tcacggctgg 33240 cgaccgccgc gatctccagg ccgggggagg cggcgaaggc gggcatcatg cgccgcaccg 33300 cgatgtccgc gcagcccagc accccgatcc gcaccggggc cccggccacc gtgcccgcgc 33360 tcaacgtgcc ctcgccggga gtgcgttgag gcaggccagc agcgtgcgga cctgcacgtt 33420 gagatagtgg ccgtggcgga cgagccaggt gagctggccg ggggtgaccc atgtgtagcc 33480 gggcggtggg tcgagcggcg cgtcgtgctc ggcggcgtcg atgatcagat agcggctctc 33540 ggcgttgcgg aaccggccgc cctcctccga gtgcacggca ccgtagcgga cacggtcggg 33600 cgacgcgtcg aggaccgagt ccaggaaggg cgggcggtcc ttctccgcga ggtgggcgta 33660 gttgcccggc gtgtactgga cggtggggcc cagctccacg gtgtccagga agccgccctc 33720 gacccgggcg tgcaccagga cgtgcgggac gccgtcgaac tcctgggtga ggaaggcggt 33780 gacgccggtg ccgaccggct cgaacagggg ttgggtccag ctggtgacct cgcggttgcc 33840 ggcctggacg gccaccccga cgacgttgaa gtagcggccc tcctcgtgct cgacggtcat 33900 ctcgtcctgc cgccagccgg gcagtccggc cagcggtacg cgttcggcgt gcacgtcgtg 33960 ccgggcgcgc tccccggtga tccaggacag cagctcgatg tcggtgagca gtgcgccgga 34020 cgccgtcgcc ggcaggggca ggcaggacag cacggtccgt gagtccatgt tgacgacatt 34080 gtcctggtgc agcagctcgc cgatctgacc gagggtgagc cagcagaagt cgtcgaggag 34140 cgggacgtcg ccgaccgcct ccacgatcat gttgcggttg gacttccgga agaaccagga 34200 gccgtgctcg gactgcaggg cgtccgcgac gacccggccg cggccggggc cgacgaagtg 34260 ctcgatgtac ttcacgtccg cgccctggtg ggccttggtg tagttgctgc gggtggcctg 34320 gaccgtgggg gagagctgga gcagattcgg gttgccgggc tccatcttgg cctgcatcag 34380 gaagtgcggc accccgtcga actccttgac gaggatgccc aggatgccga cctcgggttg 34440 cttgatgatg ggctggtacc agtcggcgta cgggccgtcg ccgtaggggc cgtcccgctc 34500 ggtgacgtgc aggccctcga ccgtgaagaa ccggccgctg cggtgcacca ggtttccggt 34560 ggactcctcg aacgaccagc cctgcaactc ggcgaacggg atgcggtcca cgcggaacgc 34620 gttggcccgg ccgcgctcgg cgagccagcc gtggaagtcg gcggtcgcca gctgggaacc 34680 ctcggtggtg gcggccgaca gcgcgagccg gtcggccagg gcggcctcgc cgcgcgatcg 34740 aagggtgtgg gacagcatgg gagaactccc tcagtcgacg tcgacgatcg tgaacaggcc 34800 cttcttgacg agcttgatgc cgtcggtggc gtccttgttc caggtgatga gcgcataggg 34860 gcccgtcccg gtcgggacct tcaccaccgt cgaccggtcc gtggacagcg ggaggcttcc 34920 caggctgacc gaggtgaagg gcgagcccgg cgggaagctg ccgtcgtccg gcatcgagtc 34980 gaagtacgcc ttcaggtcgg cctcggtcgt cccctccgcg atccgtacga agaggaactc 35040 gttgacctgg cccgggatcc ggttcacgaa ccgcacgggc tgtccggccc gcaccttgcc 35100 ccggacctcg aagcggggac cggtgcccgg cacgttcttc gacacgaccg tcgcggtcgg 35160 cgtcagaccc tggtccacgg tgctgccgga caccgtcagc cagcggtagc ggggctgggc 35220 gtcggccacc gcgaggtagt cgaacagcag gtaggtgccc ggagagaggc gctgggtgaa 35280 ggagccgggc tgcccgtcgt ggatgaccag cccgccgagc agggtggcgt cccggttcag 35340 gtccttggcg ccctggggca cctcggcagg tgtgttgccc agcgcgcgga acaggtcgtg 35400 cgtgaagtcg tcccagctca ccccgggcgt cagccggacg aggccgatcc agcccttgcc 35460 ctgctgcgtg gtcgaggcat tgaaggtggt gagcccgggc cgggtggtcg ccggcgccgt 35520 gaacccctcg gcggtggagc gcagttcgac gctgtgggag ccctcggcgg ccgaggcggc 35580 gaccgggctg ctcccgacgg gaggggcagc gaccgcgggc ccctgcacac cggccaggag 35640 cagcacgacg gcaccgccga gcccgagcgc cacccggcca ccgagcccga gcgcggtgcg 35700 cccaccgggt ccgcgagccg tgcgcccacg gagtccgcgg atcagcctgc cgccgggtcc 35760 gggcctggtc cggtccgccg tcctgccgtt cactgtcttc tcgttcatcg cgggtacgtc 35820 aacgcctttc acatgcgtgt cgggtgcggg ggcggagccg aagccgtgac tcagccgcgc 35880 gcggccacga actccttgat cgaggcggtc acgtagtcga gcatctcgtc ggtgagcgcc 35940 gggtagaccc cgatccagaa cgtgtgctcg gtgatgatgt cgctgttggt caggtcgccg 36000 accacacggt ggggctggtc gatgtaggcg ggatgccggg tgagattgcc ggcgaacagc 36060 cgccgggtgc cgatcttgcg gtcctccagg tgggacacca actccgcccg gccgaacggg 36120 gcttcggggt ccacggtgat gacgaatccg aaccagctcg ggtcggagcg gggcgtggcc 36180 tcgggcagga tcagcccggg tacgccgtcc aggccctcgc gcagccgccg ccagttgcgc 36240 ttgcgggcgt cgatgaagtc gtcgagcttg gcgagctggg tcagaccgag ggcggcctgg 36300 atgtcggtgg ccttcaggtt gtaaccgacg tgcgagaaga tgtacttgtg gtcgtagccg 36360 gccggcaggg tgcccatctg gtacttgaac cgcttgaggc acttgtcgtt ctcgcccggc 36420 tcgcaccagc agtcccggcc ccagtcccgc agcgactcca cgatccgggc gagcgccagg 36480 ttcgaggtca gcacacagcc gccctcgccc atcgtgaggt ggtgcgccgg gtagaagctg 36540 accgtggtca tgtcgccgaa ggtgccggtg agctgtccgt cgtacagcga gccgaccgcg 36600 tcacagttgt cctcgatgag gaacaggtcg tgttcctcgg cgagttgggc gatcgcggtg 36660 acctcgaagg ggttgccgag cgcgtgcgcg atgatgatcg cccgggtctt gggcccgatg 36720 gcggcggcca ccttgtccgc ggtcgcgttg tacgtcgtca ggtccacatc gacgaagacc 36780 ggtacgagcc cgttctgcag gatcgggttg accgtggtgg ggaagcccgc cgcgacggtg 36840 atgatctcgt cgcccggcct cagccgccgg tcctccagat gggacgaggt gagcgccgag 36900 acggcgagga ggttcgccga ggagccggag ttggtgagat gggccttgcg ccgcttcatc 36960 cgccgggcga acgccgactc gaatttgcgc tggctgcgcc cggcggcgat ccgcatgtcg 37020 agggcggctt ccaccagcgc cacccggtcc tccacctcga ggaccgcgcc cgagggccag 37080 atctccgtcg tgccgggcac gaactcccgt gccggtgtgc tgacttcgtg gtacgcgcgg 37140 accgcgtcga gcaccgtttc cttggggtcg ctcatcatcc tgtcctttcg tcgacgggcg 37200 gcatcacggc cgcgagcagt tcgcgcaatg aggtgtccag cgggcggcgc ggcgtccagc 37260 cgagcagttc gcgcgccagg gagatgtcca gggactgcca ctcgacgtcg ttgcggttcg 37320 gcggtgcgcc gccctcctcg ctcaccggaa cctcgaggcc gctgagcgcg atcatccggt 37380 cgatcaggtc ccggaccggc acggcctcgc cgcggcccac gttgatcacg cgccccctga 37440 ctcgcgcgcc gggcgcgacg gcagcggcca ccacggcgtc cgccacgtcc cgtacgtcca 37500 ccaggtcgcg ggcggcccgc agcggcggca gccgcagctc gtccggcttc tcgccgaggg 37560 agtcggcgcg ggcggccccg gcgagatgcc gggcgacctg gccgaagaga ctggccacgg 37620 agatcccggg gccgatcaca ttggccagcc ggagcaccac cgcgtccgcg tcctgatccc 37680 gtacggcccg cgacacggcc tgggtgccga ggagtttggt acgcccgtac ggggtgacgg 37740 gcgccggcgg ccagtcctcg gcgatggctc ctcccggagt gcccgccccg tactcgtgga 37800 cgctgccgag ctggatcagc cggggtctgc gcggctgcca ggggagtgcg gcgagcgcct 37860 cggtgacctt ggtgaccagc tcggcgttgc tggcggccat ctgaggctcg tcggcctgcc 37920 agacccgccc cgcggagttg acgacggtgt cggcgccgac cgtggcgagc aggcccgcga 37980 tctcccgggg ggaggcggcg agcacgtcga ggcggacggt ggccgtggcg cccgagcggg 38040 gtacggcgct gggggagacc aggtgcaccc gggccccgag cgcggtgaag gcctcaccga 38100 tgtggcggcc gatgaacccg gtgccgccga gcacgacgac agtgccgccg gcggccttgt 38160 cgaggtcagg caacggcccg gtccgttccg tgcttggcgg ccttccacca gtccgggttg 38220 tcccggtacc aggcgaccgt ttcggcgaga ccctgttcga agccgatccg cggggcgtag 38280 cccagctcct cgcggatctt ggtctcctcg atggagtagc gcaggtcgtg cgccttgcgg 38340 tcgggaaccc gctggatcgc ctcggcgccc gcaccggtca gttcgaggag ctgctcggtg 38400 atggcgaggt tggtgcgctc gttgccgccg ccgatgttgt agatctcgcc cgcccggccc 38460 tggttcagga ccaggtggat gccccggcag tggtcgtcca catgcagcca ctcacggacg 38520 ttggctccct cgccgtacaa cgggacccgg cggccttcga gcaggttcgt gacgaacagc 38580 gggatgagct tctcggggtg ctggtacggc ccgtagttgt tggagcagcg cgtgatggac 38640 aggtcgacgc cgtggctccg ccagtaggcg cgggccacca ggtcggagcc tgccttcgac 38700 gccgcgtagg gcgagttggg cgcgagcggc cactcctccg tccaggagcc ctcgtcgatg 38760 gagccgtaca cctcgtccgt ggacacgtgc acgacccgct ccacgccgct gtcgagcacg 38820 gcgtcgagca gcgtctgggt gcccaggacg ttcgtacgga agaagtcgcc cgcgccttcc 38880 agggaacggt cgacgtgcgt ctcggcggcg aagtgcacca cggcgtcgtg gccgggcacc 38940 agctcgcgca gcagcttgcg gtcgcagacg tcgccgcgta cgaacaccag gcgctcgtgt 39000 gaggccggca ggttgtcgcg gttgccggcg tacgtcagct tgtccagggc ggtgacctgc 39060 gcgtcctccc acccgctgta cgagccctcc agcacgttgc gcacgaagtg ggagccgatg 39120 aagccggccg ctccggtgac gaggatcctc atgccgtgac ctccactcgg gtgtggtctc 39180 cgacgaccag ccggtaccgg tcgacgccct gctcactggt gccgaccgag gcggaccgcc 39240 cgatcagcga actgcgcagg cccttgacgc cgctgaccga ggcctcgtcg agggcgatgg 39300 agttctccac ggcgctgtcg ctgaccaggc agccgcgccc gatggacgtg tgcgggccga 39360 tgtggctgtt ctccaccacc gcgcccgctc cgatgatcac cggcccctcg atgcgcgacc 39420 gtgtcacgcg cgcccccgcc tcgacgacga ccgcgcccag cagctcgctg aggtcgtcga 39480 cgtcaccctc gacggacggc gccagccggt ccaggaggta gcggttgcag tccaggacgt 39540 cctcgacgtt cccggcgtcc ttccagtagc cgccgtactg ggtggcgcgg acctcggcgc 39600 cggaggccag cagccactgg atggcgtccg tgatctccag ctcgccacgg gcgctgggct 39660 cgatcgagtc caccgcccgg tggatggccg aggtgaagaa gtacacgccg accatcgcca 39720 tgtcgctgcg cggttcgcgc ggcttctcca ccagacgcag cacctcgccg tccggcccga 39780 gttcggcgac accgaactgg cgcgggtcgg tcaccttgtg gaccacgatc tgcgcggccg 39840 gacgccgggc cgtgaactcc tcggcggtgg cggcgacgcc ctcgggcagc acgttgtcgc 39900 ccaggtacat cacgaagtcg tcgtcgccga ggaagtcccg ggcgatggcc accgtgtggg 39960 cgaggccgcg cggggcgtcc tgggagatgt aggtgagttt cacgccgaaa cgggagccgt 40020 caccgagggc ggcgtcgatc tccgggcccc ggtggccgac gatgacgccg atgtcggtga 40080 cgcccaggtc ccggatgttc tccagaacat gtaccaggac gggggtgttg gcgatcggga 40140 tgagctgttt cggcatcgaa taactgaagg gtcgcaagcg ggtaccggaa ccgcctgcca 40200 gcacaagcgc cttcatgagg agtcctctcg attcacgtgt gcatgccttt tctcgtcgtg 40260 ctccgggcgt tcgtgcctcg ggcgtcgggg ccttggcggt tcagcggagc tgttccagtg 40320 ccgccaccac gtgggagggc agcggcatcg tggcgatctc cgcggagagt tcgcccgccc 40380 gctcccggta tccggggcgt gcgagcagtt cctcgcagga gtcggcgatg cgctcggcgg 40440 tgtcctcgcc gggcagcagg gtgatcgccg ccccccgctc ggcgatgcgc agggccgggc 40500 gttccatgac ggcccccttg gggatgagca gttgggggac gcccgcgttg agtgcggtca 40560 gggtgctgac accgcccgcg tggtggacga gcaggtcgca ggtgggcgcg atgacgtcga 40620 gcgggaccca gccggcccgc aggcccggca gttcggcggt gagggcatcg gccacctcct 40680 ggggggcggc gacgatgagt tcgacgtccc acgcggtgac gtccttggcc atgccgcgca 40740 ggaactcgaa gttccggtcg tagctgtcct tggcgacccg gctgcccgag gtcacacaga 40800 tccggcgccg ctcaccgcgc gagtacatcc agggttcgag cctgcgctgc ccgttggccg 40860 ggatgtagcg catcatctgc gcgggaccgg cgttcgccgg gcgcaggctc ggcgggcaga 40920 tgtcgacgaa cagctcgggc tcgggcagcc ggtcgagccc caactcggcc agctccggcg 40980 ccagttcggt gtcggcgccc ggatggatgc cgtcggcctc gatggcgtcc caggcctggc 41040 gcacatgcgg gatgcccaga tgcgcggcga gcagcggggc gacgtagcac atcgtgccgc 41100 cgacgacgag gtccggccgc caggaccgcg cgaactccag catgcgcggg aggctctccg 41160 cgcccatgcg ggcgaaccag cggccggtga acagcgcctg ctcgacgggg tcggagggga 41220 tcgactcggg gttgccctgt cggtcctggg tgatgaactc ccggatcggc cggtgggtgg 41280 tcgggatgcc ggccagtccc gagcccgtga tgtgcggcat catgtccgcg ttcgcggcca 41340 tcacgacctg gtgcccggcg ttgcgggcgg cggtggccag cggggcgagc gcgaagacgg 41400 tcgcggggct gcccgcggtg acgaagagga tcctcacagt gttcctgcct tggtgaatcg 41460 gggcgtgcct gtcccgccga accggggcag aacgccggcg gctctggcct cggccacggt 41520 cggtgccttg gcgtccttgt cggagatgag ggggacttcg gggcagtccc acggcagatc 41580 gagctcgggg tcgagtggat tgatgtcgat ctgcgttccg ggcacatagg tgctggagac 41640 gacgtagcag atgcaggcgt cgtcggtgag cgcgagaaag ccgtggccga cgccctcggg 41700 cacatagacg gatctgcccg aatccgcgtc gagttcgttg acctggtgct tcatgaacgc 41760 cggggaaccg atccgcagat cgaccacgat gtcgcgcagc acgccccgca cacaggtgac 41820 gtacttcgcc tgacccggcg gaatgctcac gctgtggatg ccgcgcagcg tatggcgtct 41880 cgacaccgag tagttgatct ggcggggctg gaaggcgaca ccggtcgcct tctccagcgt 41940 gtcgtcacgc atcgcctcgt agaagttccc ccgctcatcg tgtatctgac ggggcgtcac 42000 cagatatgta ccgggaatga ccatttcctc gatcagcacc gcgcgttcgc ctctctcggc 42060 tcagtggcct cggggtggtg gcctttcatg cctgacgggc cagctgttcc aggtcggccg 42120 cgacatcggc gggcgacggc atcgcggcca tctccgcggc gagccgccgg gccgccgcgc 42180 cgtacgcggg atccgacagc agggtgtcca gggctccggc gatcagctgc gggtcgtcct 42240 gctcggctgc cttgtcgaag gtgaggcccg cgccggtcgc ggcgagccgg tcgcccagcg 42300 ggaagtggtc cgccagctgg ggcagggtga gctgcggcag cccgaaggcg gtcgcggtca 42360 tcgcggtgcc cgcgccgccg tggtggacca tcgcgtcgca gcggtcgagg aacaggtgca 42420 ggggcagcgg atcgatcagc cgtacgtgtt ccggcacggc gccgataccg gcccggtgcc 42480 gctcgggcac cgtcgcgagg atctcggtgc cgggccggcc cgcgagggca tgcagcaggt 42540 cccgggtgaa cggttcgccg tgcagggcga gggtgttgcc cagcgagacg gcgacccgct 42600 gccgtccggc accgggcccc cggtccgcgc gcagccagcc gggcacctca ccaccgccgc 42660 tgtagggcac gtaccggatg gacgtgcccg gctcggagtc cggcagccgc agggacggcg 42720 ggcacgggtc gagcagcacc gccggatccg gcaggccggg caggcccagc tcctcgcaca 42780 gctcccggaa gtcggggcgc accaggcgcc gggccggacc ggagatggcg tccaccgtcc 42840 aacggtggtg caccaccggc acgcccagcc gcgcgccgag gatcagcgag cagtactcca 42900 gcgggtccga gaggatcaac tcgggcccga actcccggac gagctcgccg tagtggccga 42960 gtacggactt ggcgtgcccc atccagagct tgccgtatcc gccgagcacc tcgggcgcgg 43020 gccggggccg cgcctgcaac ggccgttcct cctcggccag tccccgcagc agcatcccgt 43080 ccacgtcgaa cgactcgccc acgaccgcgc cggtcagccc ggcggcccgc acggcgccga 43140 gcacatcggg ctgcccggtc accacgacct cgtgccccgc cgagcgcagc gcccagacga 43200 gcggcacgag cggcaggaag tgggtgggaa caggggtgca gatcacgagt acgcgcatct 43260 gacggggtcc tctcggtggc tggtcggtgg gtcgccgtca gcggcgctcg gcggtcagac 43320 gttccagcag cggtacgacg tccgtgggcg ccggcgcggc gagcatctcc tcgtacaggc 43380 cgcgcgcgcc gagctggaac gacgggtcct cgaggagtcg tacgagctgc ttgtgcaggc 43440 cgtcgacgtc cagcgcggcg gggtcggcga ccaggcccgc gcccatgtcg gcgacatagc 43500 gcgccgtgac catctcgtcc cacatgacca gcggcaccac gagctgcggc acccggtggg 43560 ccacggccgc ggcgaaggtg ccgccgcctc cgtggtggat gatcgccgaa ctggtcggca 43620 gaacctggtt gagcggcacg tactcgacgg tgcgcacgtt gtcgggcacc ttgccgaccg 43680 ccgcgagctg ctcgttgttg agggtggcga cgacctcgac gtccagggcg gacacggatt 43740 cgaagaactc gcgcatcggg aagccgctgt acttgccgaa gatcttgcgc cggctcaccc 43800 ccagggtcag caccgcgcgg ggccgctcgg gccgcgcgtg cagccagtcg ggcagtgcgg 43860 ccgccccggt gtaggggacc cggcggaccg ggatgtgtgt caggtccagg ggatgccgca 43920 tccgggactg ggtcaggtcg agggtccact ggccgagcag catctcgtcg tcgagctcca 43980 gcccgtaccg ctccagcatc gggccgaacc agctcagcca cggatgctcg gtcaactccg 44040 agcgcgggtc cgccagttcg cgcttcgtgc ggtcgtggat cacggcgacg ttgtccatgc 44100 cccagaccag ccgggcgtgc gcggcgccgc tgacccgcgc ggcgatcggg gccgcgagca 44160 tcagggggtc ccacaggacc agatcggggc gccaggcgcg ggcgaagtcg accaggttgt 44220 ccaggaccgg gcggcgtccg ccctggcgct cgggcgccgg gtagtacagc gagaacatgc 44280 tggcgaggat ggagcgcgcc gtgcgccagc cgccgctctc ctccggctcg ttggggtcga 44340 agcccagcga cggacggtcc ggcttggcgt ccccggtgtg cgcaccgagc gccggggaca 44400 gctcgtccgg ggtgccgagc gacaccgcgg tgagcccggc cgcgccgatg ttcccgatca 44460 cccccgggtc caccacgccc gagtggctcg ccacgacgac ctcgtgcccg gcgctctgca 44520 gcgcccaggc aagcggcacg accgagtaca ggtgtgacgt cgcgggaaag atggtgagca 44580 ggacacgcat cgttgatctc cgttcgcgtt cgggagcggt cgtcaggtcg ccgtgtgcgc 44640 caggggagtt cgggtccaca ccgcggtgag cgcgccggcc gccgacaggg cggtgaggaa 44700 gagcagcgcc ggtcggtatc cggcaccggc cagcacggcc acggtcggca gggcgagtac 44760 cgcgccgagt tgtacggcgg tctggtagac cggcaccgcc tggcccttcc ggtcggccgg 44820 aatcccggac atggcctgca tgttgagagc ggcgaacgac agcacgaagg ccgcgccgac 44880 cagcagcagc gtcggcagca ccccggtgat gtacgagtcc ggctccccgc ccgccagcgc 44940 ggtcacggag ccgagggtcg ccgcgcagcc cccggcgacg atcagccgct cgctcccgaa 45000 acgccccacc agccgcccgg cgaagggcag cgacagcgcg agcggcacgc aggccggcag 45060 gaacgcgagc gccaaccgcc aggcgtccca gccccattgg gcgtgcaact ggtgggtgag 45120 gagcaggagc agaccgagat acgtgccgtt cagcgtcgcc gcgcagaccg cggagcggac 45180 gaaaggaccg ttgcgccaca gccgtacgag cgtctcccga agcggcggcg gaccggcctg 45240 cgacgccgcg tccgcgggaa tcagccggaa gccgaacacc agcaggagca gggcgatcgg 45300 cgctgggaag acgagatccc accgccagct cagcgcggtg agcgcccccg aggacagcag 45360 cccgaccgtg aacccggcgg caccgaacag cgagtacacc gacacggccc ggcgctgttc 45420 cgcacctgcc cggaaggtcg taccgatgat cgcgagcccc gtcggtgcgg tgagcgcggc 45480 gcacatgccc ttgacgaccc gggtcaggat cagcagcgcg ccctgatcgg ccaggccacc 45540 ggccaccgag gcggccacga agaccaggag cgccgccaga tacacgcgtc gtctgcccca 45600 gcgggcggtc acgcgcggac cgagcagcag cagggccgcg aaaccggccg cgaagccgct 45660 catcacccac tgggtgcccc acatcgagag ccccagatcg tccccgaggg ccggcagcgc 45720 gaccagcacc acggacactt cgagcgcgtc gatgagcatg ttcccggcga gcacgaacag 45780 aagggcccgg tgggcgcggc tccccttcac cgaaggggca ccggggcgtg gagagactgc 45840 cgtcatgacg tgctgaccac cttcggggcg acaggaatcc gtgcacaggc cggccgtggc 45900 tcatcggggc atcgaccgag ccacggccgg ccagtcgggg ggcgggccga ggggggcggc 45960 ccgcccggtc agcgcagcag cgtgccgccg ctggcgtcga ggtaggagcc ggtgatccag 46020 cgggcgtcgt cgctcgccag gaacgccacg acatcggcga tgtccttggt ctcgccgaca 46080 cgctcgaaga ccgagtagcg cgccatctcg gcgacggcct ccgggttgtc gaagaccggg 46140 gtgccgttgt ccgtgatgcc gggccccacg ctgttgacgg tgatgttccg cgggcccagg 46200 tgcttggcgt agtgcagggt cagctggtcg atggcgccct tggtcatcgc gtaggccacc 46260 tcctgcgggt tggcgaaccg ggtcagcccg gaggagatgt tgatgatgcg tccgccgtcg 46320 ggcaggagcc gcagggcccg ctgcacgatg aagaacggcg ccttggcgtt gacggcgaac 46380 agccggtcga actgctcggg cgtgacgtcc tcgggtgcca ccccgcccat cacccccgcg 46440 ttgttcacga cgatgtcgag agtggtctcg ccggtgcgct ccttcagccc gcgctccaac 46500 tccaggaaga gcgcgtgcac atcgccggac acgccgagtt cggccgccac ggcgaacgcc 46560 cggccgccct ccttctcgac ggcggccacg gtgtcgtcgg ctccctcggt gtcgcgggag 46620 cagtgcaccg cgaccagcgc gccctcgcgg gccagacgga tcgccgtcgc ccgtccgatg 46680 ccgcggctgg acccggtgac gagcgcggtc ctgccggtga gcttgcccat ggtgctcctc 46740 ctgtgcgtgc gggtgaacgg gcctgtggcc gtggggtgtt ctgtcctgcc ggtgtccgag 46800 acggcggggc gcgggccgtc gagcccgctg atcatcgagg cgaggtggcg gcgggcgggt 46860 tcgagcgcga gcagttcccc gaacaccgcg cgcagggcgt ccacttcggg tccgccgaac 46920 agcagctggg cctgcgcctc gatgttgccg agcacccgtg cgcccaccgg gtgccgctcg 46980 gcgtggtagc tgtcgagcat ccggtcgtcc gcgcggccgg tgatccgcgc cgcgagtttc 47040 ccgccgagcg cggccgcgtc ctggagaccg aggttgaggg cctggccgcc gaccggcagc 47100 tggactgcgc ggcgtccccg gcgaggagga cccggctgtg ccggtaactc gccgcctgcc 47160 tgcgggcgtt gtggaaggcg ttgacccaga cgggccgggc gccggtgatg tcctcgccgg 47220 tcacccgcgc ccagacccgg cagacctcgg cgaagtccgg ggcccggccg cccggggcgg 47280 ggtgcccggc cgtactggtg aaccatgagg cgggtgacgc cgtccggccc gcgccggcag 47340 ttggccaccc cgttcggatg ccgctggaag cgccggtccg ggatgtcgat tccgctgatg 47400 tccgcccgca gcagctcctt gaccggggcg ctgccgggga actcgaaacc tccgaggcgc 47460 cgtacggtgc tctgctcgcc gtcacagccc gcgaggtaag ccgctccgaa ccgcagccgc 47520 tcgccgtcgg gaccggtgac agcgacgtcg acccggtgct ccgcgaccgt cagcccggtc 47580 accgtatgcc tgcgccgcac ctcggcaccg agccccgcgg cccactcgta cagcaccttc 47640 tcgaccagtg tctgcggtgc cttccactgg ccgggaaacc ggctcgggtg cgccgcggcc 47700 aggtcgagcg ggatgccgcc gaagtggccg gggcccgcgc tcggcggtgg cccgagccgg 47760 tccagcaggc ccagaccgtc gaggatctcc atggtcctgg agtgcagtac cgacgcccgt 47820 gactcgtcgg tgggctcggc cagttgctcc agtacggtca cccgggcgcc accgaggcgc 47880 aggatcccgg cgagcaggag acccacgggc cccgcgccca cgacgaccac gtcggtgtcc 47940 ggtgcttcgg caacctgtac ggccactgct cagcgccgcg cttcggcgta cgccttggcg 48000 tggccgagcg tcgcccggct gttgttgccc agcgcgttgc ggatgaactc gcgggcctcc 48060 ggaatcccgg cctcctcgcc caggatcttg gtgatgttct ccgtgttgag gatcaccgtg 48120 tgctgcgagg tggcggtggt gacgccgtcg gccgactcgt cgagctgcca gtaaccggtg 48180 tgcaggttca tcagcgcggg cagcgtcgtc tgcttgtaga cgatccggga gtgcgggaag 48240 cacacccgga tcgactcggt ggtgtggacc gagccgtcct tggtcagcgt gtccatccgc 48300 aggacctgca ggccgggcga gtcctcggtc agcgagacct gcttgacgtg cggcagccgc 48360 tcgctccaca gctgggcctc gttgatgaag tcgtagacgt ccttggcggc gcccttgatc 48420 tccagactgt cggtgaacga cagggtcagc tcggcggagc cggtgacgcg ttcgacgttg 48480 cccttcagcg cggccagctc ggaccggctg ttgcggtcga cggcctggtc gatccactcc 48540 aggccctcgg gatcgtcgtc gacggcccgg tagtcgtgca gcaggcgcac gcgggacgcg 48600 ccctcgccgg ccggctcgat gatccacgtg ccgcccatcg acgccaccgg cggggcggac 48660 acctcctgcc ggaacgtgat gcgccgggcc tcggggtcga gcgtgcggcg cgaagtccag 48720 cccttggccg cgccgttggc ggtggcccag atgcggatgc gctcctcgcg cgcgccgcgt 48780 tcgaggtggt cgacgtggac ggtcggcggg aagatcagcg gccagttccc gacctccgcg 48840 atcagccggt acacctcggc ggccggggct tcgacagtga tctcgtgctc gacctcacgg 48900 acggtcatgg tgaactctcc tcacggataa gggatcagaa attgccgagg ccgccgcaga 48960 cgttcagggc ctgcgaggtg atggaggcgg cggtgtccga ggcgaggtag ccgaccaggc 49020 cggccacctc gtcgggcgtg gagtagcggc cgagcgggat cttcgcctgg aacttctgca 49080 ggatcgcgtc ctcggaggtg tcgtacgcgg ccgcatagcc ctgccgtacg cgctgggcca 49140 tcggcgtctc gacatagccg gggcacacgg cgttgacggt gatgccggtc ggggccagtt 49200 cgttgccgag ggctttggtg aagccgacga cgccgtgctt ggaggccgag tagggggcgc 49260 cgagcacgac accctgcttt cctgccgtgg aggcgatgtt gatgatccgg ccgcgctcct 49320 tggccgccat gccgcccgtg ttgagcacct cgcggctcat caggaacacg ctgtgcaggt 49380 tcgtggcgat gacgtcgtac cagagttcgt cggcgatgcc ggcggtggga ccgccgccgc 49440 tgcggcccgc gttgttgacc aggacgtcga tggggccgaa gcgctcgacg gcctgccgca 49500 cgaagcgctt cacgtcctcg ccctcgcgta cgtcgcagac ggcgccgtcg acgtcgaggc 49560 cctcgtcgag cagctccttg accgtgcggg tgacaccgtc ctcggtgcgg gcgcacagga 49620 acacccggtg accctgctcg gcgaggagcc gggtcaccgc gaggccgata ccgctggtcg 49680 cgccggtgac gacggcgacc ttcttctccg tgttgccgga cgtggcagtc gtcatgtctg 49740 ttgtctcctc tatctcctga cggggcgtca gctggaccgg acgggcacca ggaaggtgtt 49800 gacggcgccg atcagcgcgc gaggtgtctt cgcctccgtc accgtgtcgt cgtcgagcgt 49860 gatcccgaac tcgcgctcga tccggccgct ggtctccagc agggccaggg actcgtatcc 49920 gagagactcg aagtccgtgt cgaggatgtc gccgtccagg tcgacggcct catcggtgcc 49980 ggccccgtcg cgcagaatgc gccgaaggtc ttcgatggtg aactcgtgag cgggcatgac 50040 ggtcctttcg tcgggcggtt cgaggagaga gagcgaggcg gacgggggcg cagcggtcac 50100 ttgtcgacgg cccgtaccac cacggccgag ttgaagccgc cctgcccgcg ggcgaggacc 50160 agggccgagc ggaccgcggc gtcgcgcggg cggtcggtga ccaggtcgag ggcgtaggcg 50220 tcgctgagac gggtgccgac cgaggccggg atcacgccgt cccggatggc gaggaacgcg 50280 gcggccagat ccagcggggc cgccccggag tacagccgtc cggtcatcgt cttgggcacg 50340 ctgaccggga ccccgcgggc cccgaagaca ccggtgatcg cgtcggcctc gatgcggtcg 50400 agctcgggca gggcggcacc gtcggcgaac accacgtcga tgtcgccggc actcgactcc 50460 gcgtcggcca gcgcgagttc gatcgccttg cgcaggcccg gaggacgtcc gccgccgggc 50520 cgggggtcga aggtggagcc gtaccccgcg atctccccgt agatctgccg ggctccacgc 50580 tcgcgggccc gctccaggtc ctccaggacg agcagcgcgc cgccctcgcc cgggacatgc 50640 ccgcgcgccg ccgcgtcgaa gggcacgtac gcccgcgccg ggtcgtcgct ggtgctcagc 50700 cggtccccgg ccatctgggc gacccagccc caggggcaga tcgaggcgtc gatcccgccg 50760 gacaccacca gcggcgtgcc cttgcggatc tgccgccggg cctgtgcgac cgcgtcgagg 50820 ccgcccgcct ggtcgccgac gaccaccccg ctggggccac gcattccgtt gcggatggag 50880 atctggccgc tgttgacggc gtagaaccag gcgaacgact ggtaggcgct gacgtaccga 50940 ctgccctggc tccagagcgc cttgagttcc ttctggccga actcgaagcc gcccgaggag 51000 ctcgccgtga tgacacccat gtcgtactcg gacagctcgg cgggatcgac gcccgcgtcg 51060 gcgaacgccc agtccgtccc ggccagggcg acctgtgtca tgcggtcggt ctgcggcagc 51120 agccgggccg ggagatgctc aaggccgctg aactccttga tctcgcccgc cagtcgggcc 51180 gggtactgcg cggggtcgaa gcgggtgacc cggccgatgc cgctggcgcc ggcccgggtc 51240 gccgcccagt acgcctccac gccgaggccg ttgggcgcgg cgacaccgag cccggtgacg 51300 gccacccgta cggcggcgct catgcggcgc tcctttcggc ccggtccggg cgggccagca 51360 cgatggcgct ctggaagccg ccgaagccgc tgccgacgga gagcaccgcg tcggtctgcc 51420 agtcgcgtgc cgtgagcggc acatagtcga gatcgcactc ggggtcgggg gtgtgcagat 51480 tggcggtggg cggtacggcg ccgtgttcca tggccatcgc ggacgcggcg atctcgatcg 51540 agccgatcgc gccgagcgag tgcccgacca tcgacttgat ggagctgacc ggtacgtcgt 51600 aggcacggct gcccaggctg tgcttgaacg cggcggtctc gtgccggtcg ttctgcttgg 51660 tgccggagcc gtgcgcgttg atgtagtcga tgtcctcggg gttcagccgg gcctcgtcca 51720 tggccacccg gatggcctcc gccatctccc gcccgtcggg ccgcagtccg gtcatgtgga 51780 aggcgttgct gcgggtcgcg tacccggcga tctccgcgta gatgtgcgcg ccgcgggccc 51840 gggcggcgcc cagctcctcc aggacgaaca cggcggcacc ctcgcccagg acgaacccgt 51900 tgcgggtgcc gtcgaagggc cgtgaggcgt gctccgggtc gtcgttgcgc ggggtggtcg 51960 ccttgatcgc gtcgaaacag gccaccgtga tcggcgagat gggggcgtcc gcggcaccgg 52020 ccaccatgac gtcggcggcg ccctcccgga tcagctcggc ggcgtatccc accgagtcga 52080 ggcccgaggt gcagcccgtg gagaccaccg tgctcggacc ctcggcgccg accgcccagg 52140 ccacctcggc ggcgaacgag ctgggcacca ggtagttgta gaggtggggc acggcgtagg 52200 tgtggtcgac caggtcgagc cgcccgttgt cgctgacggt ccggtactcc tcgtcgagcc 52260 ccatggtcgc gccgaccgcg ctgccgatgg tcaccccggt ccggtgcggg tcgaggccct 52320 cgaactccag cccgctgtcg gcgaccgcct cgcgcgccgt gacgaccgcg aactgcgcgg 52380 cccggtccat gcgccggatc tcctgcgggc cgagccccag cagcgccgcg tcgaagtcgg 52440 cctccgcggc cacctgggag cggaacggcg acggatcgaa gaagctgata cgccgggtgg 52500 cggtgcgccc gtcggtcaac aggctccaga agttcttggc gcccacgccg cccggtgcgg 52560 tcaccccgat gccggtgatc acgacccgcc ggccgccggt cgcgctcaca gtgcggtcca 52620 gtggtagaag cgctgggcca tcgcgtcggc gggggagcgc caggtcgccg ggtcgtacgc 52680 ctcgatgaac ggcttgaggt cgtcgctgat cccgacgaac cgggggtccg tcttggcctg 52740 ctcgatccgc tcgccgccgt cctcgttgtc gaagtcctgc agatggaagt agagaccccg 52800 gtacgagaag agctggcggc gccgggtgcc catgaggtgc ggcatctcgg tgtcgtcgaa 52860 cctgttgaac agacgcgcga cgtcgtcggc ggatccgggc tccattcgag ccacgatcag 52920 cgtgctgtgc atctctctct ccttgtcagg acgatcggcg ccgggacggc cttgtgagca 52980 cggccggcgt caggacttcg ggccccgcga cgggccgaac cagcgctcca gcaccatcgg 53040 caggtcgtga tggctgccgg gcgcggccca cgccacatgc ccgtcggggc gcacgagcac 53100 ggctgaggtg ccggccagcg ggctgtcgtc ggacaggccg tgcggtgcgg cggtgacgat 53160 gtccacacgg tccgaccagc cggacgcccg gtcgcgcagg acagcgttgt cctccaggtc 53220 caggagcacg ccccgggccg cgcgcagcag ttcggtgctg ctggtcttgc gccggtcgcc 53280 gaccagctcc aggtgcggca tacgcaggcc gagcagggga tggcggccgc ctccgacgtc 53340 gtaccggatc tccaggccgc tcaccatgcc ggcgagatgg cggctgacct cctcgtaccg 53400 gatcagctcg cggagcacgt cccgcagcgg ctgcacctcg tcgccgctca ggaagagcag 53460 gccctgcgcc ttggtgttca tcatcaggcg ctgtccgacg gggtaccgct cgccgtggta 53520 ggtgtccagc agctcttcgg gcgcggtgcc ccggaccacg gcggccagct tccagccgag 53580 gttggcggcg tcctggacgc cggtgttcat gccctgtccg ccggccggga gatggatgtg 53640 cgcggcgtcg cccgccagca ggacccggcc gcgccggtac tcggtgacca ggcgggtcgc 53700 gtccccgaag gcgctgaccc acaccggctc ggcgtgcgag atgtcgatcc cggtgagccg 53760 ctgccaggcc gccgcgactt cctcgtacga cgggggctcg gtgcgccgct tgggcggggt 53820 gccccgctcg cagacgatga tgcgggtgat cccgccgtcg ccgagcggcc cgaccatgac 53880 catgccgccg tccacggtct ccccgatgag ccggggttcg agctcgacgc ccttgatgtc 53940 cgcgaggtac atctccatgg tcgccgccgt gccggggaag tcgaagccga cggccttgcg 54000 gacagtgctg cgcccgccgt cgcaccccac gagatagtgc gcggtcagcc gctgttcgcc 54060 gtccggcccg cgtacgtcga cctccacacc gtcggtggtg tcgcgcacgg cgagcagttc 54120 gtggccgcgg cggatgtccg tgcccagttc accggcccag ccttcgagca tggtctcggt 54180 ggtcgactgc ggcacggtct tggccgcctg gtgcacgctg tccagtacgg cgaagtcgac 54240 cggcagtcct ccgaagtgtc cggcgttgct ggtccccatg tcaccgaagc ggggcagcag 54300 cccgcgttgg tcgaagacct ccatcgtgcg tgtggtgaaa ccgaggccac gcgactcgcc 54360 ggtccgctcg gcgagccggt cgacgacgat gacgtcgacc cccgccagac gcagctcggc 54420 ggcgagcgtc aggccggtgg gccccgcgcc ggcgactatg accgaagcat ccatgtactt 54480 gtcctccggg catttcagag aggaattgca ggagaggggt tcggaatgta actggagtgt 54540 taattcagaa ccgaaataac tgaaccggca ttaattggcc gggaaacgct ttgcaccggt 54600 tggtcgacac agtgctgcgg aaactagtga ttagcttctt cgggaatcat cggaatgtca 54660 agaggagttt ttcaggcggg ccacaacatt ccgtcaagta attgttaagt cgcacttgag 54720 gatggtgagc catcgttttt ggaaggcgcg cttccggaac gcgcgccgag tgtccgctgg 54780 atgtcctcgc agtggtagta ggtgcgcccg ccctccgcgc gccttcgcca ccgctgctcg 54840 ctggcgtgcc ggtacacact gccgggcggg atgttccaca gccgggcgat gtcggcggcg 54900 ccgacccagt gcacacgggt gtcacgcgcc agcaaccggc tcaggctcag ccactcgtcg 54960 gcggaccagc ggtgggcggg tgaggcctcg cagcggatct ccagtggggt gtcggcccgg 55020 tgcggccgta cgagcgccac caggccgccc tggcagtcgg cctcgacgca ggacccgatg 55080 ggcacccggc gcagtgggct cgggtcgatc acgcgacgcg cgtcacgcac caacagggcc 55140 gtttcctggg acagttcagg ggcggcctga tgtgcggtga gccagtcgca gtgccgggcc 55200 aggaacgccg cgagggtttc cacgttcgtc ggcggagcgc tggtccggcg ctcctcgacg 55260 accacggacg accaggaccg cagcaccccg gcgatggccg accggacgtc cgccgcggcg 55320 gtgttgaagg gcaggcccgg caggggcccg cccgaggtgc gctcgcgcgg cccgtcggag 55380 ccgccgagaa gccggccgca ctcctcgtgg agccggggca gctgccgcag ttccgcgatc 55440 aggcggtccc ggcagtccgg gcagagccgg tgcccccgtg cggcgagcct gcggggccgg 55500 ccctcggccg cccgccgccc gcaccgtgca ccccggcaca cggttatttc ctgggtcctg 55560 cctgcgagta ctcgattcat ttcccccacc agttctcggt gaggtgaggc tatttccggg 55620 cctccggcac ggccgtgagt gaactcacct tctgtggggt gcgtgccgga gatctcttac 55680 ttctctgatg aatttgtcgt ggatttgaac gtgacgaaaa ttcagatgcc caggcgtgcg 55740 gagatcccgt cgaggaccga ctcgaggccc gtctcgaact gccactccag gtcgtccttg 55800 cggctcgcct cgcggaggta gcgctcgtac gtcgggtaac ggtcggtgcc catcagccat 55860 gtcatgcgtg gggccagacc ggtgcgcgcc tcgttcgcgc tggaccagcc ctgctcccgc 55920 atcagctcgt tgaggccgat ctcgaagccc acggcgccct gcacatagga gtcgacggtg 55980 cggaagaccg ccatcatcgt gtcggcgtcc aggtcgagcc cgtcgagcgc ggccagggcc 56040 cgctcgggca cggccagctg gttcggggtc agtgtgaaca ccgccgtgcg ggccacccac 56100 gagtgcctga ggagcacggt cctggtgcgc agggcgagcg cccgcatggc gttccgccag 56160 tccttggcgt ccccgggcag cgacagctcg ccgtagacgt ggtcgaccat cagctccagc 56220 agctcgtcct tgccggagac atagcggtac gccgccatcg gcgcggcccc caggtcggcc 56280 gcgaggcgcc gcatggtgac ggcgtcgagg ccttccgtgt cggcgatcgc cacggcggcc 56340 gcggcgatgc gctgcggggt gagcgtggag cgaggggcgg gtgcggggcg gttgaggcgt 56400 tcccagagcg agacggtctc cctgggctcg gacggctccg gcgtcacctg cttctcctct 56460 tttctccgag tggccatggg cggggttcct tccgggtgcc ggggttgtgt acaacgtaac 56520 agcagtagac aacgtacaca tcgatgtgta cgttgtatct gtagcgatac gccgtacact 56580 tccaggagtt ccgcatgagt gacctgaacg acagccctct gcaggtggcg gccctcgtcg 56640 gcagcacgag gggcggacgc ttcggtccgg tcgtggccga ctggctgctg agccagatcg 56700 cccagcgcaa ggacatggag gtcgacctcg tcgacctggt cgagacgccc ctgccgaccg 56760 tcatgccggc tttcggggag caacttccgg acgaggacat ggctctgcta ggcgcggtcg 56820 ccccgcgcat ggagtcggcc gacgccttca tcatcatcac gccggagtac aaccacagtt 56880 atccggcggc cctgaagaac gcgatcgact ggcacaaccc gcagtggcac gccaagcccg 56940 tcgccttcgt ctcctacggc ggcatctccg gcggcctgcg cgccgtcgag cacctgcggc 57000 tggtcctcgc cgagctgaac gccatgacga tccgcaacac cgtcagcttc cacaactacg 57060 gcgacaagtt cgactcggac ggcaagcccc tcgacccgga ctgtgacgtc gcggccaagg 57120 ccctgctcga ccaactggcc tggtggggag aggcgcttcg ggacgcccgc accgctcgtc 57180 cgtacaccgg ctgagacgaa agacggccat gaccagcgac acgacggaga ccggcggcac 57240 cgcggacacg gctccgcaga ccgccgggaa gctgccctac ctcggcatct tcggactcat 57300 gctcggcatc ttcctggcca ccctcgacgg acagatcgtc agcatcgccc tgccgaccat 57360 cgtcggtgac ctcggcggcc tcgaccactt ctcctggtcc gtcacggcct acctgctcac 57420 gatggcggcc gccactccga tctggggcaa gctcggcgac ctcaagggcc gcaagggcgc 57480 ctacatgtgg tccgtgctgc tcttcctggt cggcaccgtc ctgtgcggac tcgcccagga 57540 catgccacag ctcatcgcct tccgcgccgt ccagggcctc ggcgccggcg gactgatggt 57600 cggcgcgctg tcggtcatcg gcgtgctggt tccggcgcac gagcgcggcc gcgtccagtc 57660 gatggtcggc gtgatgctgc cggtcgcctt cgtcggcggg ccgctgctcg gcggcttcat 57720 caccgacacc ctcgactggc gctgggtgtt ctacgtcaac gtgcccttcg gtgtcctcgc 57780 gctgttcgcg gtcgccagag gcgtacggct gccgaaggag cgttccggcg gacggatcga 57840 ctaccccggt acggcgctgc tgaccaccgc catcctctcg ctcaccctgc tggccacctg 57900 ggccggcacc cgctacgact ggggatcggc acagatcgtg ctgctcgccg tcgtcgctgc 57960 cgtggccgtc gccgcattcg tccgggtcga acagcgggtg gcggaacccg tcatcccgcc 58020 gcggctgttc cgcagccgtg acttcgccct cgcccagacg ctcagcttcc tggtcggagc 58080 cgtactggtc tcggtcatca actacctgcc ccagtacatg cagttcgtgc agggctccac 58140 ctccaccgta agcggcatgc agatcctgcc cctgatgatg ggcatgctcg cggcgcagct 58200 gaccaccggg cggctgatgg accgcggcgg cctggagcgg gtggtgccga tcaccggggg 58260 cgccgtcacg gtcgtcggcg cgctgctgct gctcctgctg gacacggaca ccccggtgtt 58320 cgtcgcctcg gccctcactc tggtgctcgg catcggcgtc ggcatcctga tgcagagcac 58380 gctgctcacc acgatgaaca cggcaccgcc gcgcgacatg ggtgccgcca ccggaacggt 58440 cacgctactg cgcacgatcg gcggttcact cggcgtcgcc gcgctcggcg ccgtgcacct 58500 gggccgtatg acggacgagc tcaccggccg gctgggcgag cgcggcgccg aacagctcac 58560 ctccggcggc gagttgaccc cgtcttcggt cgacgggatg tccggtcgag tgcggggtgt 58620 cgtccaggag gccttcacca gcggactgca cggcgtcctc atcggcaccg ccctgctctc 58680 ggccgtcgcc ttcgccctcg cctggttcgt ccgtgggacc ccggccccca agacccccga 58740 acccgccggg gattcgcggc ccgcccaggt ccccgcgacg accacggact gacaccccac 58800 gttctgtctc ccagccctcc ttctttccca ccgattccgt caggcgcccc ggcgccccga 58860 ccgaaagcag acccttctca tgtcgaccac ctccgccgag cgcgccaagg aagtcctgct 58920 gcgccccttc accctgggca gtctcactct tgccaaccgg atcgtgatgg caccgatgac 58980 ccgggagttc tcgccgggtg gcgtaccggg cgacgacgtg gcggcgtact acgcgcggcg 59040 cgcggcgggc ggcgccggcc tcgtcatcac cgaggggacg tacatcggcc acgactcggc 59100 cggtaccagc gaccgcgtcc cccacatcca cggcgagcag gccctggcag gctgggccaa 59160 ggtgacgaag gcggtgcaca acgcgggcgg gaagatcctg tcgcagctgt ggcacgtggg 59220 actggaccgg aaggccggct ccccgcctgt acccgaggcc ccgagcatcg gcccctccgg 59280 catcgctgtc gacggcacgc ccgtcggccg cgccatgacg cagaccgaca tcgacgatgt 59340 cgtcgccgcc tacgccgcgg cggcagcggc cgccgagcgg accggcttcg acggcatcga 59400 ggtgcacgcg gcccacggct atctgatcga ccagttcctc tgggagcaga ccaaccgccg 59460 caccgacgcg tacggcggtg acctgggtgc ccgtacccgg ttcgccacag agatcgtcgc 59520 cgcctgccgc ggggcggtcg ccccggactt cccgatcacc ttccggttct cgcagtggaa 59580 gatgggtgct tatcaggcca ggctcgcggg atcgccgcag gagctcgaaa ccctgcttgc 59640 ccccctgtcg gacgccgggg tggacgcctt ccactgctcg acccgccgct tccacctgcc 59700 cgagttcgag ggatccgagc tcaacctcgc cggctggacg aagaaggtct cgggcaagcc 59760 cacgatcagc gtcggctcgg tcggcctcga caacgagttc atcgaggtgt tccaggggaa 59820 gccgggctcg gtcgccggga tcgacgcgct gctcgaccgg gtggagcgcg acgagttcga 59880 cctggtggcc gtcggccgcg cactgctcgg tgaccccgag tggatcacga agatcctggg 59940 tggccggacc gaggaactcc agcccttcca catggggctt ctgggtaccc ttcactgagc 60000 tcgttcacac ttcaagaatt ccaggcgacc gcatgcggca ctgattaccc gagtaatccc 60060 gtaatcaaca atcgatcacg gccactctgg ccgaatatgc acttctgata ttgcctcatg 60120 tttcccggat gcttcggctc cctcattctc gctggtgtgt caatagctat tatttgactt 60180 ttcatccagc ggtttgtagc cttctgggag cgcgactcga tgcccgctta tctggcataa 60240 catgggggag tatcggatga gcaatcagtc cacaaaattt ctcgagcccg ccattccgca 60300 acaactggcc aatcgccgcg cgccaacgcc gtcgcgccca gtgagccaac atgcccagca 60360 gcatccgccc atacaggccc agcaactacc gcccatccag gcccagcctc agctgcgcaa 60420 ggcgcgcggg gtgttgcgtg tcctcgttgt cgagagcggg gcgcccgccg cccagtccct 60480 ggttctgagt ctggccaggc acgggtacca ggccgtgagc gtggacaccg gcagtaaggc 60540 gctccagaag taccgggaag cggatgtcct gctcatcgac ctggacctgc ccgacctgga 60600 cggcatcgag atctgccgtt tcgtcagggc cgcgtccggg gtcggagtca tcgttgtcac 60660 ggcccgcgac acggaactcg accgcgtgct cagcctcaag gcgggcgcgg acgactatgt 60720 ggtgaagccc tacagtctgc ccgagctgat ggcccgtatc gaagccgtca tgcgacgggt 60780 gcgcccgctg ccactgacca ccgaagtgat cagcagcggc ccgttgcgga tcgactcgcg 60840 cacccgtgag atccggctcg gcgacgagcc cgtcgaagtg acgcgcaagg agttcgacct 60900 gctttatctg ctggcctcgc ggccgggcag cgtcgtttcc cgaaagcaaa tcatgacact 60960 ggtctggggc gattcccgct cccagccggg tcgcacgatc gacacgcatg tcagtagtct 61020 ccgcagtaaa ctcggatcca gcagctggat catcacggcc cgcggtgtcg gtttccgatt 61080 cggtcatgcg taaattggtt ccgtgtattt ccgtgaactg gaaaatggtc actcgggaag 61140 aaagcgattt cccttcgcca ggcgcataac atgaactctc ggagaaaccc tcgccgacac 61200 gcaccggcgc aaacgacgag cccgcggccc catgggacgc gggctcgtcg catggggcag 61260 gaagcctgcc ggctgccgcg agtcggcctc ggcgggtgac ttcctgatcg gccggcaacg 61320 cgagccccca ttgcccgacg gctcgacgcc gacgcggccg tgccgccgcc tcgcaccgca 61380 tccggttcca cgcgcacgcc ggttccacaa cacccgtggc ggacacgcca cctctggtgg 61440 tcgggccccg tcctcggacg acactcggac agcacgccgg cccggaagcg cacaggctca 61500 cgcttcatcc gtcctccggc ggtggccgtc acgccaccat ccgaaccgcg tggcgccgtt 61560 gtgggccgag gacttcgacc gccgcgccgg accgcagggg ccttgctccg gcccgcccac 61620 gtggatgagc gagcacgctc gggttcgccc cccgggcggg gatcccctcg cggcccaccc 61680 gctcgcgcag gaggcccgct cgtgtcgtct ccgcggcccg ggaagctgcc catggtgccc 61740 atcccggcgg ccgtccacgc acaggcgtga gcgccggacc ccgctcaccc gggtgcaccg 61800 ggtgccgttg tccggcgccg cgccggcccg ctctgccgga ggggttatgc gttacgcccg 61860 cgccgtacag cctcgagccc cagcactccg gccgcgccca cgaccacgtg catcacggcg 61920 agtgaagctg ccgtgccgcc gtcggtctcg ctcgtgaggg ggccgatggc ggtgaggacg 61980 ccgagggctc cggacacgat ctggccgacc cgcagcagca ccggcttcca gcgggccgcg 62040 aacgcggcgg ccgtgacgcc gagcgccggc ggtacgaagg aggcgaagat gacgccgccg 62100 gcgacgatgt cgtcggcctt gccgttgagc tccagggtca gggaggcccc cgccgcgtcg 62160 gcgatgaaca ggacgatcag gttgacggcc gtcgcgacga gggcactgcc caccgcggcc 62220 tgccacaggg gcagtccgcg ccccgcgcga gccgtggggg aagagctgat ctcggcgggg 62280 ttgctgctgg tcatgaaagg gtcctccggt tgatgaatga gagcgtgggg ggtcaggacg 62340 ggttcaacga gaggccgtgc ctgaagacgt tgcgcgggtc gtacgtgttc ttgacccgct 62400 gcagccgggg gtggttgtcc ttgtagagga tcgtgtacca gggcacgccc gaggagttct 62460 ggtacgggtc cctgatgtcc acgtcggggt agttgacgta tccgccgtcc tgctggtcgt 62520 tcgggaccgg gaagccgccc gtggcgctgt ggacgccctt gcgcagcgtt ctgatccact 62580 cgacctcggc cgcgtcggcc gacggctcgg accagttggt cgagtaccag accttgaaga 62640 tggagtcccg ctgggccacg gccgtcgccg acgacgacac cgtgttgatc ttgcagccgt 62700 atgccgcgag gtacacgaga ccggcgccgg tggagtgctc gggatccgtc aacgaccggt 62760 acacgacgcc gacctgagct gccgtcagcg gcttgcgcag atgcccgccg atggacttgt 62820 gccgggagta cgtgccgaac gggggcgtcg gctcggtggc cgcctccacc cagggcatct 62880 cccgtgtgtc gaccgccggc gtgacaccga caccggccga caccgcggcc aggtagtcgt 62940 cgaacagctt cggggtggcg gcgagcgtcg cgtccatccg gatttccagc acgaacgacc 63000 cgaccagcga cgaacgcagt tggaaggagc tgtgcatggt ggcgtacggc gagtcgggcc 63060 cgctgttggc cgcgtgccag gcaccgtggt tgagcagcag ccgggtgaag ccggcctcgg 63120 tgatgtccga ccactgccag gtcacaaggg acttgcgcat ccgggcgggc ggcttgggca 63180 gcagcgcggt gggggctccc gacgcgcccg gcgtgcggaa ccagaacctg gtcacgatgc 63240 cgaactgacc gccgccacca ccggtgtgcg cccaccacag gtcgcggttc gggtcgctgc 63300 ggcggctggt cgcgacgatc gtacgagcct tgccgtcgct gcccgcgacc accacctcga 63360 ccgcgtacag atggtcgacc gacagtccgt agcgccggga cagaggcccg taaccgccgc 63420 cggacacatg cccgccgaca ccgacctcgg ggcagacgcc gccgggcagg gtcacgcccc 63480 agccctcgta cagcgccttg tagacctcgc cgagccgggc gccggactcg acggcgaagg 63540 cgttcatcga gctgtcgaag tagacccgct tcatctccga ggtgtcgacg aggagacgga 63600 actgggggtc gtcaaccagg ccgtcgaagc agtggccgcc gctgcgtacg gtgatccgct 63660 ggtccttgcg gatcgcctgg gtgaccgcct tctccacctg ggaggcgttg tgcaccagcc 63720 agatctcgtc cggggcggct cggaagcggg tgttgtagcc gcgccccgcc agctcggcgt 63780 agcgcgtgtc gccgggtctg acgatcacct cgggcggaac ggccgcggcg gcggtcgtgc 63840 cggcgtgggc ggccggtgcc acggcggaca ggcccgtcgt ggtaccggcc acaactatcg 63900 aggtgccaag gaacgtgcgg cgcgaaacca tgtcgagcac tcaccctttt ctcaagtacg 63960 tggaacggct ggagactgcc gccagccaag caggggctcc acgcggtccg ggcacagttg 64020 acacggatct gtcatagctc cgtacgaaac ctgatgctca gacccaaacc cacaaaagcc 64080 cgcccctccc gaagagccac ttggcgggct gtggtcgcgg caggccacga tgggcggggc 64140 cgggcggagg gcgtggcggc aggtcagggc ctctgtcgcg aacttcggac cggacgcgtg 64200 ctcacgacgg ctccggctgc caccccagcg cccgcgagat gccgcgcgcc gcgagccgta 64260 ccgcagggat caacgccggt gtctgcgcgt cgcgttcggg caccacgacg gagacggccg 64320 cggccaccga gccgtccgcc ccacggaccg gcgcggccac cgacagggcg tcgtcggtga 64380 tctgacgggt gctcaccgcc acgcccgcgt ggcggacttc ggcgagtacg cggcgcagga 64440 cggccccgtc cgtgatggtg tgcggggtga atccggcgag atgcccgccg cagtacgtct 64500 cctggaaggc ggcctcgcag tgggcgagca gcgcgagacc cacgccggtc gcgtgcagtg 64560 gccagcgggc gcccacctgg atcttcacgc ccacggccga acggccggag atccactcta 64620 tgtagacgac ctcggagccg tcccggaccg ccaactgcac gttctcgtgg gtggcttcgt 64680 acaggtcctc cagatacggc agcgcgatct gccgcagcgc gagaccgcgc ggggagagcg 64740 ccgcgatctc ccacaggcgc aggcccacgt ggtagatccc ggacgcgtcg cgctccaggg 64800 cgccccagtc gctgagcgcg cccaccagcc ggtgggcggt ggtgagggag agcccggccc 64860 ggcggctgat gtccgtgagg gagagcgccg ggtggtcgtg gtcgaaggcg gcgagcacgg 64920 ccagcaggcg gtcgggggcg gacggcccgg tcatcggccg ggaccggtca tgagtcgagg 64980 ccagtcatgc gtcgaggccg gtcacgagtc gagttcggct tcggcgaccc gctgcagcag 65040 tgtgtgcagg gtgtcccgct cggcggggtc gaggggcagg agcaggtcgt tcgtcacccg 65100 caggcccgcc tcgtcggtgt cgcgcaggaa ggcgcggccg tcctcggtga gcaccacgat 65160 gcggctgcgg cggtcgtcgg gggaggggcg gcgctcggcg aagccgagct tctccaggtc 65220 gtcgaccaga ccgacgatcg cgctcgggtc gtagccgagc cgcgaactca gctctcgctg 65280 gagtgcgccc tccgaggtgg ccaggaaccg cagcagcgcg tagtgccgca gccgcagccc 65340 cgacgcctgc aggaacgtgt tgaacagctg ccccgaccgc agtcccagcc ggtacaggag 65400 atagccggtg tccgagtgca gcccgcgcat ccacggctcc tgcgagtcga tggcgtcggg 65460 gggcgtggcg tgctgctggc cggcgatggc gggctccctg gaccgggccc ctgcggggcg 65520 gtgcgtacgt gttccccaca gcatgccgca cagacgagtc ggcaacaact attgacgaca 65580 acaatgattg tctttagctt cgatctcgta atgctccgat ctcgtagccg tcctgccttc 65640 cgcaggtgat ccgtttctcg aagggaccgt cgtgcccagc atcgatctca ccggcaaggt 65700 cgccgtcgtc accggctccg gccgtggcct cggcctggcc tatgcgcacg ccctggccgc 65760 cgcaggcgcc tccgtggtcg tcaacgacgt ggacgaggac gtcgccgagc aggccgtcaa 65820 ggcgatcacc gaggcgggcg gcaccgccgt ggccgaggtg gtcccggtcg gcaccaccga 65880 ggccgcggac cggctggtgg gccgcgcggt gtcggagttc ggccgcctcg acatcctggt 65940 caccaacgcg ggcatccttc gggacaaggt cctgtggaag atgaccgacg aggacttcga 66000 cgcggtgatc accacccacc tcaagggcac cttcacctgc gcccgcgccg ccgccgtacg 66060 catgcgcgaa cagggcgagg gcggcacgct gatcctcgtc ggctccccgg ccggacagcg 66120 cggcaacttc ggccagacga actacgccgc cgccaaggcc ggcatcgccg ccatggcccg 66180 tacgtggtcg atggagctgg gccgtgcggg catcaccgtc aacgcgatcg tgccggtggc 66240 cgcaacggcg atgaccgaga ccatcccggc cttcgcgccc tacatcgagg ccatgcgcgg 66300 cggcgagccg ctgccggagt tcctccgcaa gggcgagggc ttcggcaccc ccgaagactg 66360 cgccgccctc gtcccgttcc tggcctccga ggccgcccgc ggggtgaccg gccaggccat 66420 cggcatcggc ggcgacaagg tcgccctgtg gtcccacccc caggagatca aggcggcgta 66480 cgctgacggt ggctggaccc ccgattccct cgccgacgtc tggccggcct cgctgggcgc 66540 cgagccgcag tcggtcggca tccccgcccc gaagttcccg gaggcgtgat ccatgacgaa 66600 catcgatgtc gaggaactcg tcgcgatcga cgtccacacc cacgccgagg tctcctccaa 66660 gggcacctcc tccctggacg acgacctgca cgaggcctcc agcgcctact tcaaggtcga 66720 gggcaaccgg aagccgaccc tggaggagac ggccgcctac taccgtgagc ggaagatggc 66780 tgccgtgatc ttcacggtcg acgccgagtc cgccaccggc accgcgcccg tcccgaacga 66840 ggaggtcgcc gaggctgccg ccgccaacgc ggacgtgctg atcccgttcg cctccatcga 66900 ccccttccgc ggcagggcgg gcgtcaagca ggcccgccgc ctggtcgagg agtacggggt 66960 gaagggcttc aagttccacc ccagcatcca gggcttcttc cccaacgaca ggtcggtcgc 67020 gtacgagctg tacgaggtga tcgaggagac cggcacgatc gccctcttcc acaccggaca 67080 gacgggcatc ggcgcgggcg tcccgggcgg cggcgggatc aggctcaagt actccaaccc 67140 gctgcacgtg gacgacgtgg ccgcggactt cccgcacctc aagattattc tggcgcaccc 67200 gtccttcccc tggcaggacg aggccctcgc ggtcgccacc cacaagccgg gcgtccacat 67260 cgacctgtcc ggctggtcgc cgaagtactt cccgccgcag ctcgtgcagt acgcgaacac 67320 gctgctcaag gacaaggtgc tgttcggttc cgactacccg gtcctcaccc cggatcggtg 67380 gatggccgac ttcgcgaagc tctccatcaa ggacgaggtc aggccgaaga tcctcaagga 67440 gaacgcggcc cggctgctcg ggctgacgaa gccgtagcgg acagccaagt gcaacgaagg 67500 acagaggcac cccatgtgcg atgaaggaca ggggcacgga catgcgcaat gagggactgg 67560 ggtcgtggcc cgcacgccgg gcccgcaaga ccccgcaccg caccgccctg atccacggcg 67620 acacgacggt cacctacgcc gggctgtacg agcgcaccac ccgcctcgca cacgccctgc 67680 gcgactccgg ggtgcgccgc ggcgaccgga tcgcctacct cggcccgaac cacccctcct 67740 acctggagac gctgttcgcc gcgggcaccc tcggggcggt cttcgtaccg ctcaacaccc 67800 gcctcgccgg acccgaactc gcctaccagc tcacggactc cggcgcgaag gccctcgtgt 67860 acgggcccac gttcggcggc ctcgtcgcgg ggctgcctgg caacagcgac gtgcgtacgt 67920 acgtcgaagt gggcgccgaa tacgaccagt tgctcgacat ggcctccgac gaggccatcg 67980 acgaacccgt catccccgac gacacctgca tcatcatgta cacctcgggc acgacgggcc 68040 ggcccaaggg cgcgatgctc acccacggca atctgacgtg gaacgcggtc aacgtcctgg 68100 tcgaccagga cgtgatcacc gacgaacgcg ccctggtctc cgccccgttg ttccacacgg 68160 ccgggctgaa catgctgacc ctgccggtcc tcctcaaggg cggtacgtgc gtcctggtcg 68220 aggccttcga tccggccgcc accttcgacc tgatcgagcg gcaccggatc accttcatgt 68280 tcggcgtgcc caccatgttc gagcacgtgg cccgccatcc gcgctgggcc gacgccgacc 68340 tgtcctccct gcggatgctc agctgcggcg gctcacccgt gccgaccccg ctcatcgccg 68400 cctaccagga gcggggcctc accttcctcc agggctacgg catgacggag gcctcgcccg 68460 gcgtgctctt cctggacgcc gagcacgcgg tcaccaaggc gggttcggcg ggcgtgccgc 68520 acttcttcag cgacgtccgc gtcgtacggc ccgacctcgc ccccgccgac atcggcgaga 68580 ccggggaagt ggtcgtacgc gggccgcatg tcatgcccgg ctactggggg ctgcccgacg 68640 agaccgctgc cgtgttccac gacggctggt tccgcagcgg ggacgccgcc cggatcgacg 68700 aggacggcta cgtcaccatc gtcgaccgcc tcaaggacat gatcatctcg ggcggcgaga 68760 acatctaccc cgccgagatc gaggaccagc tcctcgccca tcccgacatc gtcgagtgcg 68820 cggtcatcgg agtgcccgac gagaagtggg gcgaggtgcc gcgcgcggtc gtcgtgcccc 68880 gcgaggacgt cgcgctcgac cccgacgagg tcctcgcgtc cctcgccgga cgcctcgcca 68940 agtacaagat cccgaagtcg gtggtgctcg cggacgaact gccgcgcacg gcctccggca 69000 agctcctcaa gtcccgtgtc cgcaagcggt acggcaccaa ctcccagtcg aaggaagaca 69060 catgagcatc accgcgaacg gcatcgacga gctgaagaag ctcgccggca gtgacctcgg 69120 caccagcgag tggatcgagg tcacccagga gcgcatcaac acgttcgccg acgccacggg 69180 cgaccaccag tggatccacg tcgacgagga gaaggccaag gagggcccgt tcggcgcgcc 69240 catcgcgcac ggctatctca ccctctccct cttcatcccg ctcttcaccg agctgctgga 69300 cgtccagggc gtcaccacga aggtcaacta cggcctcaac aaggtgcgtt tcccctcacc 69360 ggtgaaggtc ggctcccgca tccgtctcgt cggaaagctg gcggacgtcg aggacgtggc 69420 ggggggtgtg cagatcacgg tcgacggcac gatcgagatc gagggcgcgc ccaagcccgc 69480 ggccgtgctc cagagcctgt cccgcttcta cgcgtgagcg ctccgctgtg agcgccattt 69540 cgtctgcggg ttcgtcgtgg ctggtcgcgc agttccccgc gcccctatgg ggcgcgaacc 69600 ggcaccaggt cgagaaggcg ttccgccgac tcccttgcga gtggggtcag tcgggcgtac 69660 accgtgcgga acgtcttctc ggcctgttcg gcgggccagt tgtccgggag gtgtttgacc 69720 ggtaggcgcg ggtcccggcg gacgacctgc agccactccg tcgacaggcg cagccgcagt 69780 gcgagagcct cgtcggcggc gggctccgtc gtctcccagc gctgccagcg gtgcgtgaac 69840 gcctcgtagc gcgtggccag ttcgcccagt gcccaggtct cctcgatcat ggcgccgatg 69900 tccatgcccg cgtccgcgtg ggcccggaag atcttcacat gggcggacag accgagttcg 69960 gcgacgatcg cggagacgtc ggcctcgccg ggtgcgatcc acagcccgcc gaacagcggg 70020 ccgaagccgc tccaggccaa ctgcgcgcgc agatcgtgcc gttgacgctg ccaggactcc 70080 ggcagcgaga agccgagcag cgtccaggag ccgtcccact gccggttgac ggccccgtcc 70140 tcccagatcc gctgctcgcc gtcccgcagc acggactccg agcgctcggt cagcccgaag 70200 tacatccggc gtccctcgcg ctgccgcctg agcagatcgc ggcccaccat gcgcgtcagc 70260 gtcgagcggg tcgcgtgctc gccgacgccc gcgcgcccga agacgtcgat gacgctcccc 70320 gagtacacgc agacgtcacg gccgagcacc tggtcaccca ggaacgacag catgagggac 70380 tggggccgca gcgcctcgtc agtggtcacc ggcccacggt acgcccgtgg atcagctggt 70440 gaccaggtag tacgggcgct ccggccaccc cagtacacgc ggcccccgga ccggcctcac 70500 cggtggccgg cgacctccac gaacaccggg ttggagtaga accacgtgtc gagccacgga 70560 tcaccgtcgc ccggcgcgtg cgggatcggc ccgtgcgggt cgaccgacgc gcccaggtat 70620 ccggcgccgt gccggttgcc gtcgctgccg cgcagccgca cgtagaagga ctcgtccccg 70680 gcggtcagcg ggatgcgcag ggtgtacgtg cccttgcggc cggacacgtc cgccgtgtgc 70740 acgaccttgg tggccggtgc ccgccaggtg tcccggtcgg ccgccggacc gcgcaccgcg 70800 ccgcggatga cgtccacgtg ggccagcttg ggcaggatgt cgtgcgggtt gggccgcgag 70860 gccgtcgtca cggtcacgtt cagggtgagc ttctcaccct tgcggacccg cagccgactg 70920 cccagcgtga cgccccggcc gtggtcgcag tcccgcttca gccgtacgtc gagaccgtcg 70980 agcagatggc cgtggtcgag ccagacgcgg cccgcgcgca ggcccgccat caccgcgcgg 71040 tagccgtagc gggacacgcc gacgtgcgtg cggctgaact ggccgggcca gaagtcgccg 71100 ccgggctgtg gctcggtggt gttgaccggg tcgggcagcc ggcccgtgtt gtcgaagttc 71160 tgccccggca gccagtcgcc gttcttccag gtgtcgaaca ccacccggtg cacatcggag 71220 ttggtggtga tcgtgaacag cttgccctcg gagagcatgg cgtcccacat gccgccgacg 71280 gtcgccgtca tccagtcgaa gccgccgtac gtgaggtacg agtccgtcgg ataggcgggc 71340 caggagtcgg ccgacggctt gttctcgtac tcgccgcgta tcgacgtcgg accgcgccag 71400 ccagggatgg caccgccctg cgcacccggc gcgccctcca tgccgatcat gatctcgggg 71460 gccgcgtccc gccagttccg catctcgtgc ggggagtcga tgcccaggcg catcgggtgg 71520 ttggcgaaga ccagtacgtc gtccacgtag cccgaacggc gctgtgcggc cagccacttg 71580 atggccttga cggcgtgcgc ctcgttgcgc ggggtgtcgg ggtgggtggg cgcgcccgcc 71640 gtgtagccga gcagcttgcc gtcgtaggcc agctcgaact gcgtgagcag gtccacctcg 71700 tggcggccgg gcgccgcgaa gatcgtgcag tgctcggcgc ccgggatgta ccactccagg 71760 ccctggaaga tcagctgacg cgggttcttg gcgcgtgcct tgaggatctc ctcgtgctcc 71820 agcttcgccc cgtactgggc gtgcccgaag ttggagtgct cgttgaacac catccagtcg 71880 aggccgtact tggcgctcgc ctgcgccagt tgggagaacg tgtacttcgc gtcgtggctg 71940 tagacggagt gcacgtggtg gtcgccgacg aggtaggcga ggcgcgggtc gttgccgccg 72000 aaacccttcg cgctcgccat ggcgggcgtc gccagcgatc cggcggcgaa ggcggcgccg 72060 aacaggcccg cgcggcgcat gagctgacgt cgggacacgc cctgcgcgtc gagggcggcg 72120 ggggagacgg acggatctgc ccaggcgggc agctgctgct cggtcatggt ctggggagtc 72180 cctcggagtt cgatggatca gtggttcatg aacgacgtga cgggcagggc gcgttcgacg 72240 atccgcacgt caccgagccg tccgtgcagg atctggtcga tcttcccggc gtactcgtag 72300 ccgccgagga tccagggcag gccgacggag gtgatgccga cggacgacgc cttcgggttg 72360 cgcaccaccg ggcagccctg cacatacagg gtggtgtgcc ttccgtcgtt gacgacggcg 72420 atgtgccacc acgtctccaa cggtgtctcc tggccccagt tggtcgcgat tccctgctgg 72480 ttgagggggc gcatggccca ctggggctcg cggtcgttcg acagcgacag cgtggcgatg 72540 ggttcgtccg ggtcgtcggc ggtcttcccc gcggcaccgc ccgtaccggt gcggccgagc 72600 agcccggccc acgcgtcgtg cgaggggtcc cagtcggcgg gcagccggta gaaggcctcg 72660 atggtgtaac ccgccttgaa agtggccgag ttgaggggcg cgccgtcgac cgtccgcagg 72720 tacgcgccct tcagcggggg cttgccgccg tggaagtcca ggctgccgtg gcccggttgg 72780 tcggggtggt ggtccgacga ccaggtgagc gtgccgcccg cgaccgtgac cagggagagg 72840 tcgttgccgc gcccggacac atcgcggacc gtgccggtca cggcggcccc gtcggacttc 72900 ccgtcgaagc gccagtacgc gaccgtgccc ggtatcagca tcttcgacgc gggacgcgcc 72960 gggcgcgcgg gcaccggggc gaagccggcg aagcgctcct cgaagtcgat cccgaccgag 73020 aaccggtcgg cgtcaccact gagttcgctc tcctggcgct ccagctcgtt gagccccttc 73080 gcggcccggc cgaggatcca cggcgacacc gtctccacgt ccacggtgtt ccggtcgagg 73140 tcgaagcggt agagccggat catcgccgca ccgccgaagt accggttctg atagttcgtc 73200 agatgcaggt ggacgtcatg tcccgccgcg ttcttgcgag ttgcccgcgc cgcgggccag 73260 tagtggccgt tgagggtgag gaagatctgg tcgtggtcct cgatcagctg gtcccacaac 73320 tgctgtccgt acgaggagag cgagtcgtcc tcgtcgacca gctcgtgcgt cgtgaggatc 73380 accggagtct tcgggtgctg ggcgatgacg tccttggccc acgcgaagcc cttcgccgac 73440 agccgccagt ccagcgcgag caccatccac tcccgcccgg ccgcccggaa gaggtggaag 73500 gtgttgtacc cgtctgggga ggcacccccg tacgtggacc tgcccttgaa gcggtccggg 73560 ccgaagacgt ccaggtacgg gctcgggccg cgctggtcgt ccgtcgacga cttcacgtcg 73620 tggttgcccg ccaggacgct gtagccgacg ccctgccggt cgagcagccg gaacgcctgg 73680 ccgatcgccg cgcactccgc cttcgcgccg ttctgcgtca ggtcgccgag gtgggacagg 73740 aacacgatgt tctcgtcgcg cccgtgctcc agcagatagc gcagggacgc ctcgacgggc 73800 gccttgtcga tgctgggccc gtcgaacagg tactgcgtgt cgggcatcac ggcgagcgtg 73860 aagcggcggc tgtcggggtc gggtctccaa cgggccttcg agcccggcac cgcctgcgtg 73920 gtggccgccg gcagcgcgac cgctgccgtg gccaccccga tcagcgcggt cgcccgcagg 73980 acgttccgtc gtccggcgcc ggcctgctcg gcctcgcaga gggtctggtc gtgctcatgc 74040 gaagtacaca cgtctgctcc gtgagaaggg ggacagctcg ggagaggggg gtggcgattc 74100 gagagggggt ggcgactcgg gagggggagg aggcgaccag gagcgggacg gcggccggga 74160 tcagagcacg cgcagcgtcc acgcccagcg gtgcgtgccc ggcgtgacga ggtactcggc 74220 ggtggtgtcg ggcccgcacg acgccgtgcc gagcccccgg tgcgcggcgt cgaggtgcac 74280 gacacagccg ggccgcggca ccagctcgtc gtggtgcgcg gcagccgcca ggtccccggc 74340 ccggtaccgg ttgacggaga cctgccccgg cgccccgagc tccacggtca gccccgtggc 74400 gtccggcgcc gacagcgtga accgccgcac accgtgccgg ccgccgctct cctgaggccg 74460 cagataaggg gtgaacagct cgtccaccgg aagcgagtgg tgcccgacgg gcgcgcccgt 74520 gctccggtcc gggtaggact cccaggggcc ctgcccgtac cagtccagga cgtcgagccc 74580 ggcgaccgtc tcgaagaccg tgccgacgcg ggccacgtcg tcgaggccct ccggcagttc 74640 ggccgactcc tcgacccgga ggccgccggc gaccggggtg aacacctgct cgtggcggac 74700 gggcccggcc ccggtcgcgt actcggcgac cacgacgacc cgagggccct cctcacggac 74760 gtccacgacc ttgcgtacga gctcgtcgag gccccagtcg tgccagcggg ccgccatgcc 74820 gcccagttcg tcgttgtcgg tgggcgcccg ccacagcgag aggacgggcg cggaggtcag 74880 cagcgggtgg accaggagtg cgtccgcgtc cagcgtgacg gccgggccgg acgcgggctc 74940 cggcgtcgcc tcggcgacgg ccgcgcccag ccggacctgc ggcagacaca cctcggtgcc 75000 gaggggagcc caggcctcgt cgtccgcggt gacgacccgc agcgtcagcc aggactcgcc 75060 ctcctgcacg gggaacggca gtggcaccgc cgcggactcg cccggcagta cgtcgggcag 75120 ctcggccggt gccgtcaccg tggtgccgtc cgcgaggacc aggcaccact cggcggccag 75180 ccattccagg tcccggaagt gctgccggtt gtggaccagg agcacgccct ggtcataggt 75240 gaggcggacc ggtgccgcga tctcccggtg ctcgtacatc acgggcttgg gcgtgcggtc 75300 cgggaacacc acgccgtcgg cgatgaacgc cccgtcgtgg atggtctcgc cgaagtcgcc 75360 gccgtaggcc cagcgatggc cgggcgcggc gactccgtgc tcgtacagcc cggcgccccc 75420 acgcccggca ggtcttccgt cgttcacacg ctggagaatg ccgtggtccc agaactccca 75480 gatgaagccg ccctgaagac ctggggtgga ctcgatggcc gcccaggtgt cggcgagcgt 75540 tccgttgctg ttgcccatgg cgtgcgagta ctcgcactgg atgagcggca tggtctgctt 75600 ccccgaccgg gcgtgcgcga cacagtcctc gatcggcgcg tacatcgggc agacgatgtc 75660 ggacgcggtc gtcggatccg cccagtcgag cttggccgcg ccctcgtact ggatgggtcg 75720 ggtcgggtcg tggcgccgga cccagcccgc cgccgcgtcg tggttggctc cgtagtcgga 75780 ctcgttgccg agcgaccaga tgatgaccga cgggtggttc ttgtcgcgca gcaccatgcg 75840 cgagacccgg tccacgaagg cgggcagata gcgggggtcg tcggcgatct cgtgggcgtg 75900 gtcgtgcgac tcgatgttcg cctcgtccac gacatagaag cccagctcgt cggcgaggtc 75960 gtacagcgtc gggtcgttcg ggtagtgggc ggtgcggatc gcgttgaagc cgaagcgctt 76020 catgacgacc aggtccgcgc gcatgtcgtc cgcggtgacc gtgcggccgg tcagcgggtg 76080 gaagtcgtgc cggttgacgc cccggatgta gacgcgttcg ccgttgacca gcaggtcccg 76140 gccgcggatc tcgacgtccc ggaacccgac ccggtggtgg gaggtgtcgg cgaccgtacc 76200 gtcggcccgg tgcaggcgca cggtcaggtc gtacagctcg ggtgtctcgg cggtccacgg 76260 ccatacgttc tcgacggtgc cccgcagccg ggcctcaccg aggaagtcgg acacccgcat 76320 gtcctcggcg ttgaagcggt cgaactcggt gtcctgggct aggagttggc cctccagcgc 76380 cccggtgacg taccaccccc gcggcagcgg tcccgtcgcg tcgcgcacct ggcagtcgac 76440 ccggagccgc ccgtcccgcc gcgcccgcac ggtcacgtcc gcgagataca gcgggtcggt 76500 cgcgtacagc agcaccgagc gggtgatacc ggcgtgccac cactggtcct ggtcctcgat 76560 gtgcgaggcg tcggaccact tgacgacggt gaaccgtacg gtcgccgggt caccggggcg 76620 tacgacgtcg gagaggtcga actcggccgc gaggtgggag tccttggaga tgccgaccgg 76680 tttgccgttc acatggacca ggagcacgct ctcggccgcg ccgacctgga gcacgatccg 76740 gcgtccggcc cactcggcgg ggatgtcgac ggaacgctcg tatatgcccg tcgggttggc 76800 ggcgggcgag gcgggcggga attcgcccca cggcatctgc gcgttggtgt actgcggcag 76860 gtcgtcgccg ctctgggtgg tccaggtgcc cggcacctcg gtccgggtcc actcgcccga 76920 gcagcccatg ggcgagggca gcagctggaa gtgccaggag ccgtccaggg acagcgcacc 76980 ggagcgccgg tccaccgcgt tcatggccag ccgcccccag gaggtcacct ccggtgcctc 77040 ccaggggcgc agggcgatca ggtgatcgcg tcgcatcagc gaatggctcc cttgccgaga 77100 tccgcgatga tctgcttggc gccgatcgcg aagacgatca gcagcgggat gagggccagt 77160 accgcacccg tcatgaccat cccgtagtcc gtggtgccgt ggacgccgtt gagctgggac 77220 agcgcgacct ggagggtcac gttgtccggg ttggtgaggg cgatcaacgg ccaggcgtag 77280 tcgttccact ggcccatgaa ggtgaagatg ccgaggaagg cgagaccggg ccgcacgacc 77340 ggcagcgcca cgtgccagta ctggcgcatg aagctggccc cgtcgagctt ggaggcgtcg 77400 agcagctcgt cgtggatcgc gctcttcatg tactgccgca tccagaagat gccgaacgcg 77460 ttcgcggcgg ccggcactat cagcgaggtc atcgagccga tccagccgag cttcgccatg 77520 atccgatatc tctagagccg ccccgcaggg 77550 <210> 2 <211> 465 <212> PRT <213> Streptomyces sp. SCC-2136 <400> 2 Met Leu Ser His Thr Leu Arg Ser Arg Gly Glu Ala Ala Leu Ala Asp 1 5 10 15 Arg Leu Ala Leu Ser Ala Ala Thr Thr Glu Gly Ser Gln Leu Ala Thr 20 25 30 Ala Asp Phe His Gly Trp Leu Ala Glu Arg Gly Arg Ala Asn Ala Phe 35 40 45 Arg Val Asp Arg Ile Pro Phe Ala Glu Leu Gln Gly Trp Ser Phe Glu 50 55 60 Glu Ser Thr Gly Asn Leu Val His Arg Ser Gly Arg Phe Phe Thr Val 65 70 75 80 Glu Gly Leu His Val Thr Glu Arg Asp Gly Pro Tyr Gly Asp Gly Pro 85 90 95 Tyr Ala Asp Trp Tyr Gln Pro Ile Ile Lys Gln Pro Glu Val Gly Ile 100 105 110 Leu Gly Ile Leu Val Lys Glu Phe Asp Gly Val Pro His Phe Leu Met 115 120 125 Gln Ala Lys Met Glu Pro Gly Asn Pro Asn Leu Leu Gln Leu Ser Pro 130 135 140 Thr Val Gln Ala Thr Arg Ser Asn Tyr Thr Lys Ala His Gln Gly Ala 145 150 155 160 Asp Val Lys Tyr Ile Glu His Phe Val Gly Pro Gly Arg Gly Arg Val 165 170 175 Val Ala Asp Ala Leu Gln Ser Glu His Gly Ser Trp Phe Phe Arg Lys 180 185 190 Ser Asn Arg Asn Met Ile Val Glu Ala Val Gly Asp Val Pro Leu Leu 195 200 205 Asp Asp Phe Cys Trp Leu Thr Leu Gly Gln Ile Gly Glu Leu Leu His 210 215 220 Gln Asp Asn Val Val Asn Met Asp Ser Arg Thr Val Leu Ser Cys Leu 225 230 235 240 Pro Leu Pro Ala Thr Ala Ser Gly Ala Leu Leu Thr Asp Ile Glu Leu 245 250 255 Leu Ser Trp Ile Thr Gly Glu Arg Ala Arg His Asp Val His Ala Glu 260 265 270 Arg Val Pro Leu Ala Gly Leu Pro Gly Trp Arg Gln Asp Glu Met Thr 275 280 285 Val Glu His Glu Glu Gly Arg Tyr Phe Asn Val Val Gly Val Ala Val 290 295 300 Gln Ala Gly Asn Arg Glu Val Thr Ser Trp Thr Gln Pro Leu Phe Glu 305 310 315 320 Pro Val Gly Thr Gly Val Thr Ala Phe Leu Thr Gln Glu Phe Asp Gly 325 330 335 Val Pro His Val Leu Val His Ala Arg Val Glu Gly Gly Phe Leu Asp 340 345 350 Thr Val Glu Leu Gly Pro Thr Val Gln Tyr Thr Pro Gly Asn Tyr Ala 355 360 365 His Leu Ala Glu Lys Asp Arg Pro Pro Phe Leu Asp Ser Val Leu Asp 370 375 380 Ala Ser Pro Asp Arg Val Arg Tyr Gly Ala Val His Ser Glu Glu Gly 385 390 395 400 Gly Arg Phe Arg Asn Ala Glu Ser Arg Tyr Leu Ile Ile Asp Ala Ala 405 410 415 Glu His Asp Ala Pro Leu Asp Pro Pro Pro Gly Tyr Thr Trp Val Thr 420 425 430 Pro Gly Gln Leu Thr Trp Leu Val Arg His Gly His Tyr Leu Asn Val 435 440 445 Gln Val Arg Thr Leu Leu Ala Cys Leu Asn Ala Leu Pro Ala Arg Ala 450 455 460 Arg 465 <210> 3 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Primer <400> 3 cacttcgggg gcgagtcgca cgt 23 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Primer <400> 4 gggccgtagt tgttcgagca 20 <210> 5 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> Primer <400> 5 ggatcccata tgctgtccca caccctt 27 <210> 6 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Primer <400> 6 gaattcccgc gctcaacgtg ccct 24 <110> GENECHEM INC. <120> An Enzyme Concerning in Biosynthesis of Sch47554 & Sch47555 and the Genes Thereof <130> P04-312 <160> 6 <170> KopatentIn 1.71 <210> 1 <211> 77550 <212> DNA <213> Streptomyces sp. SCC-2136 <400> 1 actagtggat catgtcgttc ttcgcgcagg acgcgacggc gatcaaggac atgcagctgc 60 tgcgcggcat cccgccgacg gagaaggccc gcaacctgat cgcctcgggc ctgaccccga 120 cggacaagct gaacatggag aacgccgact acatctccca gcgcgtcggg aaggcgacca 180 acgccctgcc cgcgccggtg ccgccgccgc agggtgccga ccagatctgg gacctgctgt 240 tccagtcgaa cctcgcggtg gccttcggca agaagtcgat caagggccag ctcggggagt 300 tcttcgacca ggcgtcgggg atcctgggcg gctgacgctc cgcgtcggcg ggcgtgtcgt 360 ccggaagccg cggaccggcc gtggctggtc gcgcccacgc ggcgagccac atatcgacac 420 agccccgcgc ccctgaaggg gcacggcagc cgccgagtag tgccgcacca ccgcaagacc 480 gggagagggc cagggagatg agtatgacca ccgcgaagac agacgaggtc gtgaaggagc 540 agcccgctcc ctcgaccccg tccgaccagc gggagcgcga ccgtatgcgc cgccgcctcg 600 ccagacggcg gggcgggggc tggtggccgt acgccttcct ggccccctgg ttcatcggcc 660 tgttcggcct gacgatctac ccgatgctgg actcgctgta tctgtccttc accaacttcg 720 acctgctgac tccggcgcag tgggtcggga ccgacaacta cagcaagatg ttcaccgacg 780 acccggtctt ctgggactcg gtgcacgcca cgctgctgta cgtcgtcgtc tcggtgccgc 840 tgaagctcgc gctcgcgctc ggtgtggcga tgctgctcaa ccgcgacctg aagggcatcg 900 gtctgtaccg ggccgccttc tatctgccgt cgctgctcgg cggggcggtc gccatcgcca 960 tcgtgtggcg gcaggtcttc ggcggcgacg gcctgttcaa cgacttcctc gcctggttcg 1020 gcatcaaggg ccaggactgg atctccagcc ccgacaccgc gctctacacg ctgatcctgc 1080 tggccgtctg gcagttcggc accccgatgg tcatcttcct cgcgggtctg aagcagctgc 1140 cgaaggatgt gtacgaggcc gccgcgatcg acggcgcctc gccggtcacc cggttcttca 1200 agatcaccct gccgctgctg accccgatcg tcttcttcaa cgtggtcctg cagatcatcg 1260 acgcgttcaa gacgttcacc cccgccttcg tcatcagcaa cggctccggc ggaccgctca 1320 actcgacgat gctgtactcc ctctacctgt acaaaaaggg cttcaccgac ctccagatgg 1380 gatacgcctc ggctctcgcc tgggtgctgt tcctggtcat cgctggcttc acggcggtca 1440 acttcatcgc cagccgctac tgggtccact acgacgactg acgcctgacg tccgagcgag 1500 gagtccttca gcccatgtcc gccatcacct cggcccacgc cccgaagtcg ccgttgcaga 1560 agctgcgttc ggccaccgga tcgcgccgca tcttcatcca caccgtgctc atcggcgtgg 1620 cgatcgtcat gctctatccg ctgctgtgga tgctgagcag ctcgttcaag cccgacaccc 1680 atatcttcac ccagccgggg ctcatcccga gctcggtgcg cccggagaac tactccgagg 1740 gctggagcgg ctcgggcaac tccttctccc tctacatcac caactcgctg atcgtcacga 1800 tcggcgcggt gatcgggaac gtgatctcct gctcgctggc cgcctacgcc ttcgcgcgct 1860 tcgagttccg cggcaagaag atctggttcg gcctgatgct cggcacgctg atgctgccca 1920 cccaggccgt gctgatcccg cagtacacga tcttctacaa cctgacctgg atcaacacct 1980 atctgccgct gatcgtgccg aagttcctcg cggtggacgc cttcttcatc ttcctgatgg 2040 tccagttcat ccgctccatc ccgcgcgagc tcgaccacgc ggcgatgatg gacggcgcca 2100 accccttcca gatctactgg aagatcatcc tgccgctgat gaagccggcc ctgatcacca 2160 ccacgatctt caccttcatc tggacgtacg acgacttcct ccaccagctc gtctacctcc 2220 agcagaacga caagttcacc gtccccctcg gcctgaccct gttcatggac cagacgagcg 2280 gctcctccta cggtgcgatg ttcgccatgt cgactcttgc cctcctgccc acgctcatct 2340 gcttcctcat cttccagaag aggctggtag aaggcttggc gacctcgggc atgaagggct 2400 gaatcccgtg catcttcccc accagcgcgg ccagttgcac cacctccccg acgacccggc 2460 cgcgtacgac gcagtgctgg ccgccgtcac cgagacggcc ctgacccgcc tcacccccga 2520 gggcaacctc gagaacccgg ccgccgtcga cgacatcggc gacacctcgc tcggcatcac 2580 gtccctgctc gcgctcgcct ggcaccgcac caaggaccca cggctgcccg aagcggtccg 2640 ccgcagcctc gccttccacc tgaactcgcg cgtctacacc gaggacaacc ccggctaccc 2700 gaacctgacg gcacgcaact cccgtctgcc gtacgcccgt tacacgctgg aggcaggcgc 2760 gcaccccatc ggcgactggc cgagcacggt gtgggcgctg ctccaggccg tcaacgtcct 2820 cgacgcggcg gacggcctcg tcgacgacga gcagtaccgc gaactcctgg acgtcgcggg 2880 cggttactgg cgctggctca ccgaggccac cttcttcaac ccgcaggacg cggcgaacca 2940 ggccatcggc tgcgtggtcg gcggcctgat gctcgcccgc catctcccgc ccgaggaggg 3000 cgagtcggtc cggcggcgtg ctctgcagct gtacgccgac gagatccgcg cccggcgtgt 3060 acgggaccgg ggcgcgcccc tgcctccgga gcacggcggc gcctacgaca acaactacgg 3120 gcccatctcc ctgtccttcc tcgcgcaggc ccaccgggtc agcggcgagg aggtcttcgc 3180 ggaggacggc gacacgctcg cccggtacat cgacgcgcgg atctgcgcga gcggcttcga 3240 catcggcggg ccgcgctaca gcgagcagca ctccgccttc gaatccgtcc tcgggctgcg 3300 gtacttcggc catcgcatcg gcgccgacat cggtcgctac gccggtgact cgcgctgggc 3360 ccggcacgcg gtcggggccg acggcggcgt ggacgggcac ttcgcgtaca tgctcgtctg 3420 gcagatccag gacaccacga gctggcaccg cacgccgagc acggcgcccg cccgccacca 3480 gctgcggtcc ggcacggtca ccgtgtcgtt cgacgaccgg atgacgccct ccgtgatcga 3540 ggccggcggc acggcgctcg tggccgccgc cgtcaaccgg cagcacgggt tcgggcccgt 3600 gacggacgac ttcctgctgt gccgtccgat gggggcggta cgggtggcgg acgtgcgcgc 3660 cgacgggctc gcggccaagc tcgtgaccaa gcccgtcgtc ggacgtgacc atgtcctgcg 3720 ccggcttcgg tcgctgtacg tcacggacgg cacgcggctg tgggccaccg tggtcgtcga 3780 acggctcgac ggggagcggt acttgctcgc cgggatgccg tatgtcgccg cggacggtga 3840 ccggttccgc cgggtgggcg acttcccgct cgtcgccgcc gccggcgagc tgcgcctgac 3900 gcaccccccg gaaggcagcc cggactcctt cgacgcccgc gccgaagtca cccagcagca 3960 ggccgcgttc gcgttggcgg ccgatccgcg tgggtacggg gaccccgacg ccgggtggcg 4020 gcacgtgcgg accacgtccg cgctggaggt cgggcccgtg ccggatgcgc cggaggggct 4080 cgacgtcttc gcggtgcggt acggggaacc ggccgccgag ccgctgaccg tggtgtgcga 4140 gcgggtggag gaggggctgg ttctgcgtac gggctccttc accgcggtgc tcggcgaagc 4200 cgacgagcat ggcgagccgc agctcacgtt gactcccgcg gggacctgac ctcctcggcg 4260 gatgcgcccg agcctccgga cagcttttgc tgcccggggg ctcgggcgtt ccggagggat 4320 ttcgcccccg ccgcccctac cctcccccac tctcggcttc gctcgagcgg ggggaccccc 4380 atcgtccctt actcaggggc tccgcccccg aacccccgcc aaaagattgc gcagttcccc 4440 gcgcccctgg gtttctaggg gcgcggggaa ctgcgcggcc agcaacagcc gacctccacc 4500 cgccaagaca ccccgcgggg tctggggcgg agccccagaa ggacgggaat gggtaggggc 4560 ggcgggggcg aaagagggtt ggtgggtcag gccgagaccg gctctcggtt cggggagtcc 4620 tggggtgccc gctcgccgag gtgttccgtc gggaccttgt gggtttcgcg ggccgtgagc 4680 gccgcgatca ccggcgggac gcacagcgcc gcggtgaaca gcgccaccga cgcccagtcg 4740 tccccgtccg gacccgcgat ctgcgcggcg aaggtcaccg cgaacccggc gatggcgaag 4800 ccgatctgcg tgccgatggc catgcccgag aggcggaccc gagtggagaa catctcgccg 4860 tagaaggacg gccagacacc gttcgcggcg ctgtagacga caccgaaggt gacgatgccg 4920 aggaccatga ccagcgggta ggagcccgtg gagatggccc acaggtaggc gaccatcagc 4980 acgccactgc ccgcggcgcc gatcaggaag acgggccggc ggccgatgcg gtcggagagg 5040 gttgcccaga gggggatggc gcccagggcg accaggttgg cgagcgcacc cacccacagc 5100 atggacgtac ggtccatgcc gacggagtcg ctggtggcgt acgacagcgc ccacaccgtg 5160 aagatcgtgc tgaccgaggc gacgagcgcg gccgcgacca ccctgaggac gtccgcccag 5220 tggtcccgca gcagcacggc gagcggcatc ttcgcgacgc cctcggtggc ggcctgctgg 5280 gtgaaggtcg gggtctcctc cagcgtgcgg cggatgacgt agccgacgac ggcgaccgcg 5340 atgctcatcc agaacgggac ccgccagccc caggagagga gttggtcctc ggggagcgcg 5400 gcgaccggga tgaacaccag cgtggcgagg agctggccgc cctgcgtgcc gctgagcgtg 5460 aagctggtga agaagccgcg tttgttgggt ggcgcgtgtt ccagtgacat ggagttggcg 5520 ctggcctgct cgccggcggc cgagatgccc tgcaggacac ggcagatcac caggagcacc 5580 ggggccagcg tgccgacctg gtcgcgcgtc ggcagacagc cgatgaggaa cgtcgagagg 5640 cccatcagga tcagcgtgaa gaccatgatc ttcttgcggc cgaccttgtc gccgaagtgc 5700 ccgaggaaca gcgcgccgac cggcctggcc gcgtacgcga cgccgaacgt ggccagcgac 5760 agcagggtcg cggtggccgg gtccgactcg tcgaagaaga cctcggggaa gatcagcgcc 5820 gccgcgctgc cgtagatgaa gaagtcgtag tactccagcg cgctgccgat ccaggcggcc 5880 gtcgcagcct tctttggctg gccgggtggc gcgtcgaggg gcgctgcggg gacggacacg 5940 gcttgctcct tcgggggaac tccaacgtaa gcggagtgag cggaggggga gaagtgccgg 6000 atccctgggg tgatggggcg gccgtgccgg gggctacccg gctaattaac ccactggata 6060 gttagtagcg gttggctacg gatgttgcgc tcacgtttcc cgggtgtcaa gaggccgtac 6120 cgaaggatct tgcggtgccg ttcccgcgtc ccgcccgcac cgtgccgggc ccgcatcgcg 6180 ccgcgccgag ctttcagtcc gtcgagcctt cgggctctca gtccgccgag cggtccgccg 6240 tcagataggc gatgaccatg tcgcccagca tgttccggta gtgctcgcgc tgggccgggt 6300 cgaccaggtc gcggccgaac agggccccga aggtgtgccg gttggcgacc cggaagaagc 6360 agaacgagct gatcatcgcg tgcaggtcga cggcgtccac gtcggcggtg aagaggcccg 6420 actcctgccc cgactccagg atgcggcgga tcacgtccag ggcgggcgag ccgatcttgc 6480 cgagcttctc ggaggcggcg acgtgctcgg caccgtggat gttctcgatg ctgaccaggc 6540 ggatgaagtc ggggtgcgcc tcgtggtggt cgaaggtcag ctcggccagc cgccggatgg 6600 ccgcgaccgg gtccaggtgc tccacgtcga gcccctgctc ggcctcgcgg atgacgctgt 6660 acgcccgctc cagtaccgcg gtgaacagct ggtccttgcc gccgaagtag taatagatca 6720 tccgcttcgt ggtccgggtg cgggcggcga tctcgtcgac cctggccccg tcgtagccgg 6780 cccgggcgaa ctcgtgcgtg gcgacgtcga ggatctcggc ctgggtgcgg gccgcgtcac 6840 ggatgcgccc gttcggtcgt gccggttctt cgacgctggt catcgggctt ccttcgggcg 6900 aggggcgtgt gcccgagatt ctagaagcag gcttccgcgc acggttccgg gttacgggcg 6960 tgcacggttc cgggttgtcg gccgggcctt cccgtggctc gcggatgctg gtatagctaa 7020 cgtactagtt cgtacattag tgagccgctc tggaggtccg tcgtggtcgc catggtcaag 7080 gactcgtatc tcgtcgggct gatcggcgcc gggatcggcc cgtcgctcag cccggcactg 7140 cacgagcggg aggccgaccg gcagggcctg cgctatctgt accggctgat cgacatcgac 7200 gcgctcggtg tcgggccgca ggcggtgggg gacctcgtac gagccgcccg cgacctgggc 7260 ttcgacgggc tgaacatcac gcatccctgc aagcagctcg tcatcgggca tctggacgcg 7320 ctcgccccgc aggccgaggc gctcggcgcg gtgaacaccg tcgtcttcga gggcgggcgt 7380 gcggtcgggc acaacaccga tgtcaccggg ttcgccgcct cgttcgcccg tgggctgccg 7440 gatgccccgc tggagcgggt cgtgcagttg ggcgcggggg gagcgggggc ggccgtcgcg 7500 catgccatgc tcacgctcgg ggccgggcac gtcaccgtcg tcgatgccat gccggaccgg 7560 tcggcggacc tcgccgcctc gctgaaccgg cacttcggtg cggggcgggc cgctgccgcg 7620 ggcccggagc ggctggcggc gctgctcggc ggtgcggacg gcatcgtgca tgccacgccg 7680 acggggatgg ccgctcatcc ggggctgccg cttcccggtg agttgctgca tcccgggttg 7740 tgggtggccg aggtggtgta ccggccgttg gagaccgagt tgctgcgtgc cgctcgggcg 7800 gcggggtgtg cggttctcga tggtgggggg atggctgttt tccaggccgc ggacgcgttt 7860 cggctgttca cggggcggga gccggacgcg gtgcggatgc ttgcggatat tgcggagttg 7920 gccggtgtgg ccggtgccgc aggcgccgcc ggggcgcgga gttgagggcg tctcgggggt 7980 gaggggcttg gacggtgcgg gcccttcggg gttgatcgcg cagttccccg cgccccttgg 8040 atgcagggcc tcagccgtgt cgtgaagtgg aaccggagga gcatcaacgt gcgtacgtcc 8100 attgccaccg tctctctcag tggggccctt accgagaagc tcacggccgc ggcgcgggcg 8160 ggcttcgacg gtgtcgagat cttcgagaac gacctgctcg ccagccccct cacgcccgag 8220 gaggtgcggg cccgctgtgc cgatctcggg ctgagcatcg acctctacca gccgatgcgt 8280 gacatcgagg cggtgccggc ggaggggttc gggcggaatc tgcggcgggc cgagcacaag 8340 ttccggctga tggagcggct cggggccgac accgtgctcg tgtgctccag cgtgtcgccg 8400 ctcgccgagg acgacgacgc gctggccgcg ttccagctgc ggcggctcgc cgaactggcc 8460 gaggggttcg gcatccgcgt cgcgtacgag gcgttggcgt gggggcggca cgtcagtacg 8520 tacgaccatg cctggcgcat cgtcgaggcg gccgaccatc ccgcgctcgg gacgtgcctg 8580 gacagttttc acatcctggc ccgcggttcc gatcccaagg ggatcgagga cattcccggc 8640 gagaagatct tcttcctgca gctggccgac gcgccgctga tggcgatgga cgtgctgcag 8700 tggagccggc actaccgctg cttccccggg cagggcggct tcgatgtcgc cgggctcgtc 8760 gggcatgtga tgcgtgccgg atacgaaggg ccgctctcgc tggaggtgtt caacgacgtg 8820 ttccggcagg cggaggccgg gccgaccgct gtcgacgcgt tgcgttcgct gctcgtgctg 8880 caggagagcg tcggcctcac cgcgctgccc gctcctgccg taccgaccgg tgtcgccttc 8940 gcggaactga tcacccccga tgtcgaaccg ctgtcggaga tcctcgaagc gctcggcttc 9000 gcccgcacgg cccggcaccg cagcaagccg gtcgacctgt ggcagcaggg tgaggcccgg 9060 ctgctgctca acacgggtcc ggccgcacga cgggacggta cccagctggc cgccgtcggt 9120 ctggagtccc cggatccggc gggcgcggcc cggcgggccg aggcgctgct cgctcctgtg 9180 ctgccgcgcc ggcgcgcccc cgaggacgcc ccgctggacg cggtcgccgc acccgacggc 9240 acggaactct tcttctgcgc cacggaccga cccgaactgc ccagctggac cggcgacttc 9300 gagcccaccg cgcacaagtc gcccgtcgtg cacacgtcgc ttgtcgcgca cgagtcgccc 9360 gtcgccgggg gcatacaccg catcgaccat ctggccctca cccagccctg gcaccagttc 9420 gacgaggcgg ccctcttcca ccacagcgtc ctcggactcg gcgcgcagga gagcgtggac 9480 gtcgccgacc cgtacgggct gttgcgcagc cgtgccgtca ccaacgccga cggcagtgtg 9540 cggatcgccc tcggtgtcgg agcggccccg agcgccgacg agggcggccg ggcccagcac 9600 atcgccctcg cgaccggcga catcgtcact gcggcccgcc ggttccggga cgccggaggc 9660 cgcctcctgc ccatccccgc gaactactac gacgacctcg cggccaagta cgagttgggt 9720 gacggcgagc tggaggcgta ccgcgaactc ggcatcctct acgaccggga ggcggacggc 9780 gccttccggc actgctacac ggagacgatc ggccgcgtct tcttcgagct ggtccagcgt 9840 gacgctggct accgcggcta cggcgcgcag aacgccccgg tgcggctcgc cgcccagcac 9900 gtacggcggc cggtgcgctg actccacacc ggagggcgct ctctcaccgg attctcaacc 9960 tccgccaggc cgggcccgat cgcaggggcc cggcctgcgg catgcgctag cactgagcat 10020 tggcggtatg caagcaggcg gacattaccc acgagttgac attgaaacgt caagagtcgc 10080 cgctatcatc actccactac ggagcgaccg tccgccgccg accaccacca cggctgcacg 10140 gaatcggcca cacctcccac ttacacctgg cgcggtcggg catgcccgca cgcactcgtg 10200 caggacggaa agcagcgcat ccatgggcaa caacaggggc agagggctcc aggccaatgt 10260 cctcggcacg ttcgacacgg tcgtgatggc gatcgccggc agcgccccgg cgtactcgct 10320 cgccgcgacc accgcggttc tcgtcggcgc ggtgggtctg gccagccccg cggcgctgct 10380 gtactgcgcg atacccatgc tgggcatagc cctggccttc agctatctca gccggatcga 10440 caccaacgcg ggcgccagct actcctgggt cggccgcacg ctccacccct tcctcggctt 10500 catcagcggc tgggcgctgg tcgtctcggc caccatcttc atggtcgccg gttcgctgcc 10560 cgccggttcg atgacgctgt ccctcttcga cgaggggctc gcggacaaca cggccctgtc 10620 caccctggtc ggcgcggcct ggttcgtcgc catgctgctg gttgtgctgg gcggcgcccg 10680 gctcaccgtc cgcgcgcagc tgatcatgtc cggtgtcgag ctcgccatcc tggccctctt 10740 cgccgtcggc gccgtgctcc acaccgacaa tgcccggccc ttcgactggt cctggctcgg 10800 cttcagccac ttcgacgggg tgagcggctt cgcgtccggc gcgctcatcg ccgccttcta 10860 ctactggggc tgggacgtca cctccaacct cagcgaggag accaaggaca gccggcgcac 10920 cacgggcctc gcgggcctga tcggcgtggg catcgtcttc ctcctcttcg aggccttcac 10980 catcgcggtg aacgtcatcc tcacttcgca gcagatccag gacaacgacg ccaacgtcct 11040 cgcggtgctc ggtgaggaga tctggccggg ctggggcggc aagctgctga tcgtctccgt 11 100 gatgctgtcc accatcgcca ccctggagac cacgctcatc caggtcacgc gttccctgtt 11160 cgcgatgggc cgcgaccaca cgatgcccag cgccctgggc aaggtccacc gcacgtggaa 11220 caccccgtac gtcgccatca ccgtggtcgg cgcggtcgca ctgctgatgc tcgtggcctc 11280 caacgccctc ggcagcgtcg gcgacatcct ggagaacgcc atcgccgcga tcggcctcca 11340 gatcgccgtg tactacggcc tcgcggggct cgcggtcgtc gtcgcctatc gcaagatgct 11400 gctgaagtcg ccgtccaact tcctcttcgg cgggctgtgg ccgttcctcg gcgccctgtt 11460 catgttctgg atcttcatcg agtcactgag cgatctgagc aacgcctcga tcgccatcgg 11520 catcgggggc ctggccgtcg gactcgtccc catgctctgg tactggctcc agggcagcga 11580 ctactaccgg ccggcggacc tggacgccag cgacgtcgtc gagacggact acatacccga 11640 cggctacacc gccgccgagg cggaacgttc ccgcgtccac gagggtctcc ccaccgactt 11700 ctgagagggg tcccgatggc gagagaccgc ttcaagcccg agttcgaccc cgactgcggg 11760 gacgcgcccc tcacctccgc caaacaggac atcgtcatcg gccgctggca ggggctgcgg 11820 gatctgctgc ggaccaccgg ccccgtgtgg cttgcgaggg gccaccggat gcggctgctc 11880 gcgcaggcct gcgcgagcac ctcgacggcc gagtcctggc tggccgctga gccccacagc 11940 ggtgacgcgc tggtgctgcg ggccgcgacc gagacggcac gggccttcaa cctggccatc 12000 gccgcgggcc ggggtgtccc catcgacctg aaccgcgtcg acggcgcggt catggcctgc 12060 ctccaggccg ccgaggcgta cgtcgacgac cccactccct ggatctcgct catcgccgtc 12120 gcgcggctct atcccaccgg ggtgcggcgc caggaactgg gacgctggtg ggacgagctg 12180 cacgcccgcg acccctacag cgtggagggc catctccagg tgctgcacta ctactcggcg 12240 cgctggcacg gtacccacgg cgtgatgtac gacttcgccc gggacgcggc cggggtggct 12300 ccgccggggt gtgcgctgcc ggtgctcgtg cagttcgccc gggtggagga gtaccggtac 12360 atcctggaag gggcgaaggg gcagcagggg tctctgaccg gggtcagtca gcactggaat 12420 catgacgggg ccgtcagtga tgtgcggcgt acctggcagc ggtggatcac gggccgtccc 12480 gacgcgcccg tcgagccggg ggaaatccac cagctcaacc acctcacgca cgccgcctgt 12540 cacgccgggg tgccggacat caccgcctca ctgttccgcc tcctcggccg acgggccacg 12600 cggacgccgt ggtcgtacac gggtgacccc gagaaacaaa tcacgagatg gcacaacaac 12660 ccgaagcacc gtgcgtgagc agtcgagggt agtttttcgc ccccgccgcc cctacccatt 12720 cccgtccctt acttgggagc cagcccccaa acccccgctc ctcaaacgcc ggaggggctg 12780 gggggagggg gctgctgggg gtgggtgggt tggccgtggg tgggcaatcg gcctggaggc 12840 ctcgtcctca atcgccggac gggctgggtg gtgggggctt gcggggtggg ttgtgggtgc 12900 gggttggctg ttgttggccg cgcagttccc cgcgccccta aaaacctagg ggcgcgggga 12960 actgcgcagt cttttggggg ttcgggggcg gagcccctga gtaagggacg atgggggtcc 13020 ccccgctcga gcgaagccga gagtggggga gggtaggggc ggcgggggcg aggaaactac 13080 cccggcggca aggtgttcca ctcgctgacg accgggcggt cgtgttccag ggacaggcgg 13140 ctcaacgtgc cggttccgag tcggaacagg cgcccgtccg cggcgggcag cccgagccgc 13200 cgcgccgtaa ggacacgaag gaagtgggcg tgcgccacaa gcaccacgtc accgtcgccg 13260 agcgatttct ccgcccgcgc gaggacacga tcggcccgcg caccgacctc gtcggcagac 13320 tcgcccggat gcccgtccga ccccgccggc accccgtcgt tccacaagta ccagtcggga 13380 cgagtgcggt ggatgtcgac cgtggcgata ccttcgtacg ccccgtagtc ccactcgtgc 13440 atgtccggat cggacaccgc cccggtcagc cccgccagct ccgcggtccg tatcgcccgc 13500 ccgagcggac tggagagtac gagcgcgaag tgccgcccgg cgaacagcgg gacgagggac 13560 ttggcctgtt cctcaccgcg ctgggtgagg ggcagatcgg tccagctggt gtgccggccc 13620 gacctgctcc actcggtctc gccgtgccgg acgagaagga ggtcgcccat gactactgac 13680 tcctacttcg cggactcgac ggcgtggccg ccgaactggt tgcgcagcgc cgcgaccatc 13740 ttcatctgcg gcgagtcgtc ctgccgggac gagaaccgcg cgaagaggga ggccgtgatg 13800 gcgggcagcg gcacggcgtt gtcgatggcc gcctcgacgg tccagcggcc ctcgccggag 13860 tcctccgcgt aaccgcggag ctgctgcagg tgctcgtcct cgtccagcgc gttgaccgcc 13920 aggtcgagca gccaggaacg gatgaccgtg ccctcctgcc aggagcggaa cacctcgcgc 13980 acatccgtga cggagtgcac cgcttcgagg agctcccagc cctcggcata ggcctgcatc 14040 atggcgtact cgatgccgtt gtggaccatc ttcgagaagt ggccggcgcc caccttgccc 14100 gcgtggacga acccggcgtc gccctccggc ttgagggcgt cgaagatcgg ctggaccttg 14160 gcgatgtgct cggcgtcgcc gccgaccatc agggcgtagc cgttctgcag gccccagaca 14220 ccgcccgaga caccggcgtc gacgaagccg atgcccttgg ccgccagctc ctcggcgtgc 14280 ttctcgtcgt ccgtccagcg ggagttgccg ccgtcgacga cggtgtcgcc cggctccagc 14340 aggtcgccga gttcgtcgat gacaccctgg gtggcggccc cggccgggac catcacccag 14400 accacgcgcg gtccttcgag cttgccgacc agttcggtca ggctgctgac gtcggagagg 14460 tccgggttgc ggtcgtagcc gatcacggtg tggcccgcgc ggcggatccg ctcgcgcatg 14520 ttgccgccca tcttgccgag gccgatgagc ccgagctgca tcgcagtcat gtcagttcac 14580 ttctttcgca gagtgcggta cgtggccacg agggcggcgg tggagggatc gagaccgggg 14640 acgtcggcgc cctcggtgag ggcgggttcg acgcgcttgg cgaggacctt gccgagctcg 14700 acgccccact ggtcgaagga gtcgatgttc cagatcgcgc cctgcacgaa caccttgtgc 14760 tcgtagaggg cgatgagctg gccgagcacc gacggggtca actcggcggc caggaccgtg 14820 gtcgtggggt ggttgccgcg gaaggtgcgg tgcgggacct gctcctcggg caccccctcg 14880 gcgcgtacct cgtcggcggt cttgccgaac gcgagggcct ggccctgggc gaagaggttc 14940 gccatcaaca ggtcgtgctg ggcggcgagt tcggcaccca gctcgtcgac cggttgacga 15000 agccgatcag atcggccggg atcgtcttcg tgccctggtg ggagcaactg gtagtacgcg 15060 tgctggccgt tggtgccggg cgtgccccac acgacgggcc cggtctgcca ctcgaccggg 15120 tgtccctcgc ggtcgacgga cttgccgttg gactccatgt cgagctgctg caagtacgcg 15180 gtgaacttgg acaggtagtg cgagtacggc agcaccgcgt gcgactgggc gccgaagaag 15240 tcgccgtacc agacgcccaa caggccgagc agcaaaggcg cgttggactc ggcgggcgcg 15300 gtcctgaagt gctcgtcgac gaggtggaag ccgtcgagca tctcccggaa gcggtccggg 15360 ccgatcgcga tcatcagcga cagaccgatc gccgagtcga acgagtaacg gccgccgacc 15420 cagtcccaga actcgaacat gttggccgtg tcgataccga agtccgcgac cttctcgacg 15480 ttcgtcgaca gcgccacgaa gtgccgggcc acggcctcgg actcaccgcc gagaccggcg 15540 agcagccagg tccgcgccga ggtcgcattg gtgatcgtct cgatggtcgt gaacgtcttc 15600 gaggcgacga cgaacagcgt ctccgccggg tccaagtcgc ggacggcctc gtgcaggtcg 15660 gccccgtcca cgttggacac gaagcggacc gtcagatcgc ggtcggtgaa ggcgcgcagg 15720 gcctcgtacg ccatcgccgg accgaggtcg gagccgccga tgccgatgtt gacgacattg 15780 cggatgcgcc tgcccgtgtg gccggtccac ttgccggagc ggacctgctc ggcgaagtcc 15840 gacatcttgt cgagcacggc gtgcaccttc ggcacgacgt tctcgccggc gacctcgacg 15900 accgcttcga gcggggcgcg cagcgcggtg tgcagcacgg cccgcccctc ggtgacgttg 15960 atccgctcgc cgcggaacat cgcgtcccgc agcccgaaca cgtcggtggc ggtggcgagt 16020 tcctggagca gcgcgagcgt ctcgtcggtg atcaggtgct tggagtagtc gatgcgcagg 16080 tcaccgaccc gcacggtgta gcgcgaggcg cgctccgggt ccgcggcgaa cagctcgcgc 16140 agctgcggat ggaggagcgg gcccgcgcgg tggtcctcca gggccgtcca ctcggggcga 16200 cgggtgagcc tgggcgtccg gggctcggac ggaggcgtgt caggcacggc tctcctcctc 16260 tcccgagtcg tcaccgcgca gggcgacggc gtacatctcg tccgcgtcca ggcgccgcag 16320 ctcctcggca atcagttcgg aggtgctgcg gaccttcagc gcgagggtgc gcgagggctg 16380 gcccggcagc gacagcgtcg cgaggggacc ctcgggacgg tcgatgacga tctcgccgtt 16440 cttggtgccg agccggacgg cggtgacgac cggccccgcg gtcacgaccc gctcgaccgc 16500 gacctggagg cgggcctcca gccagcgggc cagcagttcg gcgctcgggt tctcggcctc 16560 gctctccacg gcggccgagg tgatcttcgt acgggcctgg tccagggcgg cggcgagcat 16620 ggaacgccac ggagtcagcc gggtccaggc caggtcggtg tcgcccggcg cgtacgaggc 16680 ggcacgggcc tccagggccg cgagcggtgc ctcgaccgcg tacatgtcgg tgatccggcg 16740 ctgtgcgagg gcgcccagcg ggtccttggc cgggacctcg ggcgcgtcca ccggccacca 16800 gacgacgacc ggcgcgtccg gcagcagcag cggcaggacc accgagtcgg cgtgctcgga 16860 cacctccccg tacagccgca gcacgaccgt ctcgccggtg cccgcgtcgg cgcccacccg 16920 gacgtcggcg tcgaggtgcg agttcgtgcg gtcgcgcggg gtgcgtgcgt gccgcttgat 16980 gacgaccagg gtgcgcgagg ggtgctcgtg cgaggcctcc tcggccgcct tgatcgagtc 17040 gtaggcgttc tcctcgtccg tgacgatgac catcgtcagg accatgccca cggcgggcgt 17100 gccgatggcg cggcggccct ccactagggc cttgttgatc ttgcttgccg tggtgtcggt 17160 caggtcgatc ttcatggcct gcgccagctc cgtccgtctc gtgcgagcat ctcgtcggct 17220 tccccgggac cccagctgcc cgaggcgtac tgcgcgggcc tgccgtgctt gtcccagaac 17280 tcctcgatcg gatcgaggat cttccaggac tcttccacct cctggtgtcg ggggaagaga 17340 ttggcgtcgc cgagcaggac atccaggatc agccgttcgt acgcctccgg gctggactcc 17400 gtgaacgact cgccgtaggc gaagtccatg gagacgtccc ggatctccat cgacgtaccc 17460 ggcaccttcg atccgaagcg caccgtcatg ccctcgtcgg gctggacacg gaagacgatc 17520 gcgttggcgc cgagctcctc cgtggcggtc gagtcgaacg gggagtgcgg ggcgcgctgg 17580 aagacgaccg cgatctcggt gacccgccgg ccgagccgct tgccggtccg caggtagaag 17640 gggacgcccg cccagcggcg gttgtcgacc tccagcttga cggccgcgta cgtgtcggtc 17700 ttcgacgcgg ggtcgatgcc ctcctcttcg aggtagccgg gcaccttggc gccgccctgc 17760 cagccctccg cgtactgccc gcgcacggtg tgctcgccca ggtcctccgg cagccgcacc 17820 gacttgagga ccttgagctt ctcggtgagc agcgagtccg cgtcgaaggc gatgggctcc 17880 tccatcgcgg tcagcgccat cagctggagc aggtggttct ggatgacgtc acgggccgag 17940 ccgatgccgt cgtagtaccc ggcccggccg ccgatgccga tgtcctccgc catggtgatc 18000 tgaacgtggt cgacgtacga ccggttccag acgggctcgt acatctggtt ggcgaagcgg 18060 agcgccagga tgttctggac ggtctccttg ccgaggtagt ggtcgatccg gaagacctgc 18120 tccggctcga acacgtcgtg caggaccttg ttcagctcac gggcgctctt gaggttgtga 18180 ccgaacggct tctcgatcac cgcgcgccgc caggaacccg ccggcgcgtc ggccagcccg 18240 tgcttcttga gctgcttgac gaccttcggg aagaacttcg gcggtacgga gaggtagaag 18300 gcgtagttgc cgctggtgcc ccgggaggcg tccaactcct cgacggccgc gcgcagttgc 18360 ttgaacgcct ggtcgtcacc gaagtcgccg gggatgaacc gcatgccctc ggcgagctgc 18420 tgccagacct cctcgcggaa ctccgtacgg gcgtgctcgc gcaccgagtc gtggacgatc 18480 tgggcgaagt cctcgtcctc ccagtcgcga cgggcgaaac cgacgaggga gaagcccggc 18540 gggagcagtc cgcggttggc caggtcgtag acggccggca tcagcttctt gcgggccagg 18600 tcgcccgtga caccgaagat gaccagaccg gacggcccgg cgatcttcgg cagccggcgg 18660 tcgccggggt gacgcagggg gttgtcccag tcggacgcga ggtccagcgc ctgggcgagg 18720 gactcgggcc cgaggtcggc ggcggtggcg tccggcgtcg taccggccgt ttccgctgcg 18780 ggggtcccgg tctccgctgc cgttccggat gccttcgccg gggccttctt gggacgcttg 18840 tcgctcattc tccgtcaact cccttgcggc tcagtgagac ggcgaccgcg tcgagcagtt 18900 cgccccaggc ggcctcgaac ttggcgacgc cctcgtcctc cagctgccgt acgacctcgt 18960 cgtacgagat gccctgcttc tcgacggcct ccaggtcggc gcgggcctgg gcgtagccgc 19020 cggtcaccgt gtcgccgcgg atctcgccgt ggtcggcggt tgcgtcgagc gtggcctccg 19080 gcatggtgtt gaccgtgccg ggcgcgacca gctcgtcgac gtacagcgtg tccttgtagg 19140 acgggtcctt gacgccggtc gaggcccaca gcggacgctg cttgttggcg ctcccgccga 19200 gggcggtcca gcggtccgag gagaagacat cctcgtacgc ctcgtacgcg agccgcgcgt 19260 tggcgagcgc cgccctgccc ttgagcgcga gggcctcgtc cgtgcccacc ttcgccagcc 19320 gcttgtcgat ctcgctgtcg acgcgggaga cgaagaagga ggccacggag tggatcgtgg 19380 agaggtcgag gcccgcggcc cgtgccttct ccagaccggc caggtaggcg tccatcacct 19440 cgcggtagcg ctccagcgag aagatcagcg tgacgttgac gctgatgccg cgcccgatga 19500 cctcggtgat cgcggggaga ccggccttcg tcgccgggat cttgatcatc acgttagggc 19560 ggtcgaccag ccaggcgagc tgcttggcct cggcgatggt cgccgtcgtg tcgtgggcga 19620 ggcgcgggtc gacctcgatg gagacccggc cgtcccggcc gccggtcgcg tcgtacaccg 19680 gacgcaggat gtcggcggcg gcgcggacgt cggcggtggt catcatccgt acggcctcgt 19740 cgaccgtcac gccgcgtgtg gcgaggtcgg ccagctgctc ctcgtaaccc tcgcccgagc 19800 cgatcgcggc ctggaagatg gaggggttgg tcgtgacgcc gacgacgtgc ttcgtctcga 19860 tgagctcggc gaggttgccg gacgtgatcc gcttgcgcga gaggtcgtcc agccagacgg 19920 agacgccttc ggcggagagg ttcttgaggg tttccgctgt tgtggttgct tcggtggtcg 19980 cggttgcttc ggtcacagtg atcatcttcc ttcgggcgat cggatcaacc gcggactgcg 20040 gcaagagatt cccgcgcggc ggaggccacg ttctcggggg tgaagccgaa ctcggcgaac 20100 agggtcttgg cgtcggcgga ggcgccgaag tgttcgaggg agacgatgcg tccgtggtca 20160 ccgacgtacc ggtaccaggt caggccgatg cccgcctcga ccgccacgcg ggccttcacg 20220 gacggcggaa gcacgctcgc tcggtactcc ggcgactgct cctcgaacca ctcgacggac 20280 ggcatcgaca ccacacgcgt tccgacgccc tcggcctcca actgctcgcg cgcggtcacg 20340 gcgagctgca cctcggagcc ggtcgcgacg aggatcacgt ccggggtcgc ggtggaggag 20400 tcgcgcagga cgtaaccgcc cttcgccgcc tcggggttgg cctcgtacgt cggcacgccc 20460 tgacgggtga gggcgaggcc gtgcggggcc gggttggtgg cgtgccgctt gaggatctcg 20520 gcccaggcga tcgacgtctc gttggcgtcg gcggggcgca cgatgttcag gcccgggatg 20580 gcgcggagtg aggccaggtg ctcgaccggc tggtgggtcg ggccgtcctc gccgaggccg 20640 atggagtcgt gcgtccagac gtacgtcacc ggcagctgca tcagggccga aaggcggacg 20700 gcgttgcgca tgtagtcgga gaagacgagg aacgtgccgc cgtagatccg ggtgttgccg 20760 tgcagcgcga tgccgttcat ctccgcggcc atcgagtgct cacggattcc gaagtggacg 20820 gttcggccgt acgggtcggc ctccggcagc gggttgccct tcgggaggaa ggacgaggtc 20880 ttgtcgatgg tcgtgttgtt cgagccggcg aggtcggcgg agccgcccca cagctcgggg 20940 agtacgtcgc cgagcgcctg gaggaccttg ccggacgcgg cacgggtcgc gacggacgtg 21000 cccgcctcga acgccgggag cgcggactcc cagccctcgg ggagctgacc ggccacgatc 21060 cggtcgaaga gcttggcgcg cgcgggctgc gcggtgcgcc actcggccag ctgcttgttc 21120 caggcggcgt gcgcctcggc accccggtcg agggcccgcc gggcgtgggc gagaacctcg 21180 tcggcgacct cgaaggtctg ctccgggtcg aagcccagga ggcgcttggt ggccgcgatc 21240 tcctcggcgc cgagcgccga gccgtgcgcg gcctcggtgt tctgggcgtt gggcgcgggc 21300 caggcgatga tcgtgcgcat cgcgatgatc gaggggcggc cggtctcggc cttcgccgcg 21360 gtcagggcct cgtggagcgc gcgcgggtcg atgtcgccgc cgagcgcggg ctcgatccgc 21420 tgcacgtgcc agccgtacgc ctcgtaccgc ttcagtacgt cctcggagaa cgcggtggcg 21480 gtgtcgccct cgatggagat gtggttgtcg tcgtagacga agacgaggtt gccgagcttc 21540 tggtggccgg cgagcgagga cgcctcggcg gagatgccct cctccaggtc accgtccgag 21600 acgattgccc agacggtgtg gtcgaagggg gactcgccct cgggagcctc ggggtcgaac 21660 aggccgcgct cgaagcgggc ggccatcgcc atgcccaccg cgttggcgac gccctggccg 21720 agggggcccg tggtcgtctc gaccccggcc gtgtgcccgt actccgggtg tccgggcgtc 21780 ttcgagccgt gcgtgcggaa ggccttgagg tcgtcgagct ccagctcgta cccggagagg 21840 aagagctggg tgtagagggt cagcgaggtg tggccggggg agaggacgaa gcggtcgcgg 21900 ccggtccact cgggatccgc gggatcgtgt cgcatcacct tctgaaagag ggtgtacgcc 21960 gccggtgcga ggctcatcgc ggtgcccggg tggccgttgc cgaccttctg cacggcgtcc 22020 gccgcaagca gacgggccgt gtcgacggct cgccggtcca gatcggtcca ctcaaagctg 22080 tcaggtgtct gcgtgctcat cttcaagaag tcctcggtag aagcccagta actgctcggt 22140 tgcgttcaaa cttaaaagtc agacttttgc cggggaaggt ggccctgcgt cagcctttgg 22200 tgaaagtggg acaaggactg cgggaccgag tcgacgcgag agggacatgg cggacataag 22260 cacccaagac gccgacgccg gcgacgggat caggaccttt ccctttcccg tcgacctgag 22320 cgtctgcgga gtcggcatgc aggtcggtcc gatggagccc ggccggacct ggcacgcgaa 22380 cgaggcgatc gagcgcgtcc accgcatcga cttccacgtg gtcatgctct tcggggacgg 22440 cccggtccgg cacatggtcg acttcaccga gtacgaggtc tccgccggtg agctcctgtg 22500 gatacgcccc ggacaggtcc accgtttctc cagcacggag gcctaccgcg gaacggtcct 22560 gatcatgcag ccgggcttcc tgccccgcgc cacggtcgag tcgaccggcc tctaccgcta 22620 cgacctgccg cccctgctca ggcccgaccc ggcccagctc gccggactga gggccgccct 22680 cgcccagctg gaacgcgagt acgtcgacac gaccacgctg ccgctcagcc tgcacacctc 22740 cgtgctgcgg cactccctga cggcgttcct gttgcgcttg gcgcacctcg cggccagctc 22800 cgccgacgcg gcacgcgacc aggaccaggc ggacaccacc ttcacccgat tccgcgaggc 22860 cgtcgagaag ggcttcgcca ccaaccacag cgtcagcgcg tacgccgacg ccctcggcta 22920 ctcccgccgc accctcgtcc gggcggtccg cgccgcgacg ggcgagaccc cgaagggctt 22980 catcgacaag cgggtcgtcc tggaggccaa gcggctcctc gcccacacgg acatgccgat 23040 cggccgcatc ggggtctcgg tgggcttccc cgacgcggcc aacttcacca agttcttcca 23100 ccagcacacc ggggtggccg ccggcggcgt tcagggctga actgcactga gaaatcagag 23160 ggattggacg aatccgagga atcgaaggaa ggagggggcg ggcctgtccg gcacccctcc 23220 tcaccgcact tggccctcag cgggcgaagc tgaaccagtt cacgttcacg aagtccgccg 23280 gttggccgct cgtgaaggtc agatagacgt cgtgcgttcc ggtgacgccg ctggtgttcg 23340 tcgggacggt ccgccagctc tgccagccgc cggtgttggc gagcgcgaag gtgccgatcg 23400 gtgcgttcgt acggctgtcg aggcggactt cgaccaggcc actggcgccg ccccccacac 23460 cgctcgcgac ccgggcgctg aactgcctgg ccgccgtgga gccgaagttg acgcccttgt 23520 actgcaccca gtcgccgttg gcgagggcgc cgatgttctg gccgccgcca gagtcggtcg 23580 tggtctcggt gatagtgccg ctctgaccgt cgaacgactc ggcctggatc gcgctgtacg 23640 cgtcacggtt gccggtcggc ggaggtgtgg tgccgcccgc ggactgcagc acctggacgt 23700 agtcgacgac gagtgggttg ccgggcacgg tggcaccgtc cggaccgccg ccgaaggcgt 23760 ccacgaaacc gccgcccatc gccacgttca ggatgatgaa gaagccatgg ttcgtggcgt 23820 tcgcccaggt cgtggcgtcg acctgattcg cccgtacggt gtggtagttg atgccgtcga 23880 cgtagaagcg gatctcctcc gggctcgtcg accggtccca ctccatgccg tacgtgtgga 23940 agccggcctg gcaggtcgcg cccgggcagg cggtcgagcc gccgaggccg gtcgtctcgt 24000 tgcaggggcc gcccgggctg gagccgcaat gcatcgtggc ccacacggtg ttgagaccct 24060 ggacgttctc caggatgtcc aactcgccga cgctgggcca gttctggtag ttgccgcggt 24 120 agggcgcgcc cagcatccag aacgccggcc agtagccgcg ggcggcggcg cccgtcacgt 24180 tcggcatctg gatccgggac tggacacgca gtttgccacc ggcgggcggc tggaagtcgg 24240 tgcggttggt ctcgatgcgg cccgaggtcc agttgcccgc ggcgtcgcgg cgcggggtga 24300 tccggaggtt cccggcgccg tccagcgcca cgttgttggt gctggacgtc atgttctcga 24360 tctcgcccgt gccccagttg gagggcccgc ccgggtagcc gtggccggtc gcgtactgcc 24420 agttcgaggt gttgacgccg gtgcccgccg tgccgttgaa gtcgtcgacg aagacctggg 24 480 tccagccgct gggcggcgcg ggcgcgtccg cctctgccgc cgatgccccg gccgccgcca 24540 ggctgagcac gctgacgagc gcgatgagcg tgcgccggag tggaccgcgt ctgacgggtg 24600 tgcctgaagt ttccctcatg ggtgcctctt cgggtacgga gtgggggtgc gcgggtggtt 24660 cgggggtggt cccgaggtgg ttcggggtgg ttcgggggtg gtccggggtg gttcgtgggt 24720 acgcgagagc atttgagagc gctctcaacg cgaccgcgcg gccaatgtgc tcttcggcac 24780 tccggtcgtc aagagataaa gcagagaaag ctcttggggg gcaggggagt tcacaccgtg 24840 aaggggcgga tcggacgcgg cgtgagagag cgctccgtgc cgttcagttc gtccacgacg 24900 ggttccagcc gttgaggccg tcggccggac gcacaaaagc cccccggtac cgcggctcag 24960 gccgcggtac cggggggcgt cgaagggtgt tctacgaaga gcaggacgtc acgctcagcg 25020 cgagccgagt acggtccagg cgtcgccgcc ggcgagcagc gcggagaggt cgcccttgcc 25080 gttctgttcg atggccatgt cgagttggtc cgacatctgg acgtcgtaga cgggccggtc 25140 gacggagcgg aacacaccga tgggggtgtg gtgcagggtg tccgggtcgg cgagtcgtga 25200 gagcgcgaac gcggtggtgg gggaggcgga gtgtgcgtcg tggacgagga tctgcggctc 25260 gttctccggg gtgacggtga cggccttgag gtcgccggtc cgctcatcgc gtacgacacc 25320 gcggttgccg tcggcgccga agcggatggg cttcccgtgc tccaggcgga tcaccgcctc 25380 ctcggcctgc tgcctgtcct tcagcacctc gaaggcgccg tcgttgaaga tgttgcagtt 25440 ctggtagatc tcgacgaggg cggtgcccgg gtgggccgcg gcctgccgca gcacctccgt 25500 cagatgcttg cggtcggagt cgacggtccg cgccacgaat gacgcttccg cgccgatcgc 25560 gagcgacacc gggttgaagg gtgcgtccag ggagcccatc ggggtcgact tggtgatctt 25620 gccgacctcg gaggtggggg agtactggcc tttggtgagg ccgtagatcc ggttgttgaa 25680 gagcaggatc ttgaggttca cgttgcggcg cagggcgtgg atgaggtggt tgccgccgat 25740 ggacagcgcg tcgccgtccc cggtcaccac ccacacgctc aggtcgcggc gggaggaggc 25800 gagtccggtg gcgatggcgg gggcgcggcc gtggatggag tgcatcccgt aggtgttcat 25860 gtagtacggg aagcgggagg agcagccgat gcccgagacg aagacgatgt tctccttggc 25920 gaggcccagt tcgggcatga agccctggac ggccgcgagg atcgcgtagt cgccgcagcc 25980 ggggcaccag cgcacttcct ggtcggactt gaagtccttc atggactgcc tggcctcggc 26040 cttgggcacc agggtgagcg cctcgatcgt gcccgggcct tccgtggtcg tctcagccat 26100 cgatggcctc cttgagagcc gtggcgagtt gttcggcctt gaacggcatt ccgttgacct 26160 ggttgtagga gtgggcgtcc accaggtatt tcgccctgac cagggtggcg agctggccga 26220 ggttcatctc ggggatcacc accttgtcgt aacgcttcag caccgtgccc agattcgccg 26280 ggaacgggtt gaggtgccgt agatgcgcct gcgcgatcgc ttccccggcc gcgcgcagcc 26340 gccgcacggc cgcggtgatg ggtccgtagg tggagcccca gcccaggaca agggtgctgg 26400 cgccgtgcgg gtcgtcgact tccacatccg gtacgtcgat gccgtcgatc ttggcctggc 26460 gggtgcggac catgaagtcg tggttggcgg ggtcgtagga gatgttgccc gagccgtcct 26520 gcttctcgat gccgccgatc cggtgttcga gaccgggtgt tcccgggatg gcccacgggc 26580 gggcgagggt ctgggggtcg cgtttgtagg gccagaagac ttcggtgccg tcgtccaggg 26640 tgtggttggg gccctgggcg aactgcacgc gcaggtcggg gagttcgtcg gtgtccggga 26700 tccgccacgg ttcggatccg ttggccagat agccgtcgct cagaaggaac accggggtgc 26760 gataggtgac tgcgatacgg gcggcctcga tcgcggcgtc gaagcagtcg gcgggggtcc 26820 ggggggcgac gatcggcacc ggtgcctcgc cgttgcggcc gaacatggcc tgcagcagat 26880 cggcctgttc cgtcttcgtc ggcagaccgg tggaggggcc gccgcgctgg atgtcgatga 26940 ccagcagcgg cagttccagc gacaccgcga gcccgatcgt ctcgctcttg agcgcgacac 27000 ccggtccgga ggtggtggtg acggcgagcg agccgccgaa ggcggcgccc agtgcggcgc 27060 cgatgccggc gatctcgtcc tcggcctgga aggtccggac gccgaagttc ttgtgcctgg 27120 acagttcgtg caggatgtcg gaggccgggg tgatcggata cgagcccagg tagaggggca 27180 ggtccgcctg ctgggaggcg gcgatcaggc cgtaggacag ggccaggttc ccggagatgt 27240 tgcggtaggt gccggtcggg aaggccgcgg tggccggcgc gatctcatag gagacggcga 27300 agtcctcggt ggtctcgccg aagttccagc ccgcgcggaa cgcggcgatg ttggccgccg 27360 cgatatcggg cttcttggcg aacttggtgg tgaggaactt ctcggtgccc tcggtcggcc 27420 ggtggtacat ccagctcagc agccccagcg cgaacatgtt cttgctgcgc tcggcctcct 27 480 tgcgggtgag gtcgaactcc ttgagggcct cgacggtcag cgtggtgagg gggacggggt 27540 ggacgctgta gccgtccagc gaaccgtcct gaagggggct cgtggcgtag ccgaccttct 27600 gcatcgcccg tttggtgaac tcgtccgtgt tgacgatgat ctccgcgccg cgcggtacgt 27660 cggcgatgtt cgccttcagg gcggccgggt tcatcgccac gaggacgttg ggcgcgtcgc 27720 cgggggtgag gatgtcgtgg tcggcgaaat gcagctggaa agacgaaacg cccggcaggg 27780 tgcctgcggg cgcgcggatc tcggcgggga agttcggcag tgtcgaaagg tcgtttccga 27840 aggatgccgt ctcggaggtg aagcggtccc cggtgagctg cataccgtca ccggagtccc 27900 ccgcgaacct gatgatcacc cggtccagcc ggcgtacatc cttcgacggc aggggggcgg 27960 aatcatcaag ggcgcctgaa tgattcggga ggtcgggcct ggtgaacacg gagtctccct 28020 aggaattgat ttcgcagatg agggcgccgg aggtgacgga ggcgccgact tcggcggaca 28080 gggccttgac ggtgcccgag cggtgggcgt tgaggggctg ctccatcttc atggcctcca 28140 ggacgacgac gaggtcgcct tccttgacct cctggccctc ttcgacggcg accttgacga 28200 tggtgccctg catgggggag gcgagggtgt cgccggaggc ggcggggccg gacttcttgg 28260 ccgcgcggcg tttgggcttg gcgccggctg cgaggccggt gcgggccagc gacatgccca 28320 gggagacggg cagggagact tccaggcgtt tgccgccgac ctcgacgacg acggtctcgc 28380 ggcccggctc gtcttcgacc tcggtgtccg cgggagcggc gaagggcttg atgtcgttga 28440 cgaactcggt ctcgatccag cgggtgtgga ccgtgaacgg gtcggtggag ccggtcagtt 28500 cggggcgaag ggcggggtcc ttgacgaccg cgcggtggaa ggggatcgcg gtcgccatgc 28560 cctcgacgtt gaactcggcg agcgcgcggg cggcccgttg cagggcctgc tcgcgggtgg 28620 cgccggtgac gatgagcttg gcgaggaggg agtcccaggc cgggccgatg acgctgccgc 28680 tctccacgcc cgcgtccagc cgcacgcccg ggccggacgg cggggcgaag gtggtcaccg 28740 tgccgggagc gggcaggaag ccgcggcccg ggtcctcgcc gttgatgcgg aactcgaagg 28800 agtggccgcg cagttccggg tcgccgtagc cgagctcctc accgtcggcg atgcggaaca 28860 tctcgcggac gaggtcgatg ccggcgacct cctcggtcac cgggtgctcc acctgcaggc 28920 gggtgttgac ctccaggaag gagatcgtgc cgtccgtgcc gaccaggaac tccacggtcc 28980 cggcgccgac atagccggcc tccttgagga tggccttcga agaggagtac agctcggcga 29040 cctgctcgtc ggagaggaac ggcgcgggcg cctcctcgac cagcttctgg tggcggcgct 29 100 gcagtgagca gtcgcgggtg gagacgacga ccacgttgcc gtgggagtcg gccaggcact 29160 gggtctccac gtgccggggc ttgtcgaggt agcgctcgac gaagcactca ccgcggccga 29220 aggcggccac cgcctcgcgg acggccgagt cgtacagctc ggggacttct tccagggtgc 29280 gggcgacctt cagaccgcgg ccaccgccac cgaaggcggc cttgatggcg atcggcagcc 29340 cgtgctcctc cgcgaaggcg acgacctcgt ccgagcccga gaccgggtcg ggggtgccgg 29400 cgaccagcgg ggcgccggcg cgctgggcga tatgggcctt gtcacccagg tcgcgaatcg 29460 cctgcggcgg cgggccgatc cagatcagct gggcgtccag gacggcctgg gcgaagtcgg 29520 cgttctcgga aaggaagccg taaccggggt ggatggcgtc cgcgccggag tccttggcgg 29580 cctgcagcac cttggccatg tcgaggtagc tggtcgcggg ggtgtcaccg cccagcgcga 29640 acgcctcgtc cgcggcccgc acatgcagtg cgtcccggtc cggctcggcg tacaccgcca 29700 cgctcgcgat accggcgtct cggcacgccc gggcgacacg aacagcgatt tcgccacggt 29760 tggcgatcaa caccttgcgt atgcgggcct gacggaggcc taggagggcg gcgctcatgt 29820 acgccaactc tgtggcgcgc cgtacccgga ggtatggccc atacgctgcc atccggctcg 29880 ccgcgatgcc gcacgggcac cgtcgccgtc cggggcggga cgtcgcgcac tgccggcgag 29940 gaggacggct gtcagggcgg cgagctcctc cgcgccggcc tctcccttca acacctggat 30000 ggacggaacg ctggtggtca cgaactctcc tgtcactgtg gggggttgcc gtgtttgcgg 30060 gagggcagtt ccgcgtgctt ggtgcgcagc atcgccaggg agcggatgag taccgtacgg 30120 gtctcggcgg ggtcgatgac gtcgtcgacc aggccgcgct cggccgcgta gtacggatgc 30180 atgagctcgg ccttgtactc cttgaccagc ctggcgcgca tcgcctcggg gtcgtcggcg 30240 gcggcgatct gcctgcggaa gacaacgttg gcggcgcctt ccgcacccat caccgcgatc 30300 tcgttcgtcg gccaggcgta ggtcaggtcg gcgccgatgg actgcgagtc catgacgatg 30360 tacgcgccgc cgtaggcctt cctgaggatc agtgagatcc gcggcacggt cgcgttgcag 30420 taggcgtaga gcagtttcgc gccgtgccgg atgacgccgc cgtgctcctg gtccacgccc 30480 ggcagaaagc ccggcacgtc gagcagcgtc accagcggga tgctgaacgc gtcgcacagt 30540 tgcacgaagc gggccgcctt ctcgctggcc gcgatgtcga ggacgccggc catggactgc 30600 ggctggttgg cgacgatgcc caccacctgg ccgtcgagcc gggcaagtgc gcagatgatg 30660 ttgcgggccc agcgctcgtg gacctccagg tagtccccgt cgtcgacgag ttccccgatc 30720 accttcgtca tgtcgtacgg ccggttgccg tccacgggga ccaggtcaag cacggcgtcg 30780 gtgcgccggt ccgcccggtc gtcggggtcc acccggggcg ggacgtcgcg gttgttctgc 30840 ggcagcatcg agagcagata ccgcacctcg gcgatgcagg tctcctcgtc gtcgtacgcg 30900 aagtgcgcga cgccgctcgt ctccgcgtgg acgtccgcgc cgcccaggcc gttctgggtg 30960 atctcctcgc cggtgaccgc cttgacgacg tcaggtcctg tgatgaacat ctgcgaggtc 31020 tcgcggacca tgaacacgaa gtccgtcagg gccggggagt acgcggcgcc gccggcgcac 31080 gggcccagca tcacgctgat ctgcgggatg acgcccgagg cgcgggtgtt gcgctggaag 31140 atgccgccgt agccggcgag cgcggacacg ccctcctgga tgcgggcgcc cgcaccgtcg 31200 ttcagggaca ccaggggtgc gccggccgtg atggccatgt ccatgatctt gtggatcttc 31260 gtggcgtggg cctcgcccag cgagccgccg aaaatgcgga agtcatgggc gtacacgaag 31320 accgtgcgcc cgtgcacggt gccccagccg gtgatcacgc cgtccgtgta cgggcggttc 31380 gcctccaggc cgaagccggt cgcccggtgc cggcgcagcg gctccacctc gctgaacgtg 31440 ccttcgtcca tgaggagttg gatgcgttca cgggctgtca gcttgccgcg ggcgtgctgg 31500 gccgaggtcg cgcgttcgcc gggaccctcg tgcacctgcc tcttgagcct ggccagctcc 31560 tcgacgtgcg tggccgtcgg ccgccgctcc tccatgatgg tcatacgtgt gccctcccga 31620 tcagtgactg gtcagggacc ggagatcccc gggcagttca cgcacgacgg tgtgcgtggg 31680 cggtgtgccg tggagcgcga cggccgccgc gtggcccgcc gggcacggca cgtcccgtac 31740 ggtccagccg tccggccctc gcggggcttc cgggccgccg gcgccgacgt acacctcgga 31800 catccagcgg ccgattccgg cgcccgtgcc tttcaggtat gcctccttgc gcgcccagac 31860 gcgggcgaag cccgcctgcc ggtccgctcc ctccaggcgt cgcagttccg cctgctcgta 31920 cggatgcagg gagggcaggc agacctccac ccgctcgggt gtcggcagcc gctccacgtc 31980 gacgccgacc gggcgggcgg ccacggcgat gagcgccgtg ccatggctgt gcgagagcga 32040 gaagtgcggg acggagccgg gcgcggcgag tacgggcctg ccgtgcgggc ccccgcaccg 32100 ggggcagtcc gcacgggcga aggcgagctt gcgcgcgggg atcccgacgt acgtggcgag 32160 cacccgcctc agcgcgatgt gcgccgccac gtacagatcc cggtcggccg ggcgccggaa 32220 ggcggcggcc cgccgccgct cggcctcgtc gagctcgtcg agttggtcga gggcgccggg 32280 tccgtacgag gggaacgcgg caccgtcctc ctcaggccga cggctcggag acagcagaaa 32340 gacttccaac gtgcttgtcc acacgtgttg ttgacgttcc atcacccgat gctcccgctc 32400 gtcgcacgtc actcagcagc accgcctgcc gtagcgtgtc ggcgctcggc gaggtgcccg 32460 cccgcaccgc cgacatgaag gccctgacgg tggccgcgac ctggtcggcg ggccggagcc 32520 ggatctcctc gatgtccgcg ccacgctgca cggtgatcac ggggatgtgg tcggccggcg 32580 gggtgaaggc gcggtcgacg gtgatccggc cctcgctgcc ccacagttcg tacccggagc 32640 ggtatgcgtg ctccatgccg aaggtgatct gcgcggtgac tccgccgggt gtgcgcagca 32700 gcgcggcgcc ggacgtctcg acgaggcgcc ccgcgccgtt cgcgagatgg gcaccgacga 32760 cctcgacgtc cggcccgagg aagtgcaggg cggcccgcag cggatagagc cccacgtccg 32820 ccagtgcgcc gccgccgagc tcggggacgt accggatgtc gtcgtcgggg agccggggga 32880 tggtgaacgc cgcctggaag gtgcggagtt caccgatcct gccgtccgcg acgaggcgcc 32940 gcaccgcctc gtgcacggga tggtggacga acatcacgtt ctccatcagc gcgagccccc 33000 gggcggcggc gaggcacagc agccgttcgg tggtcgcgga atccgtgctg aggggcttct 33060 cggcgagtac gtgcaggccc gcgtcgagcg ccgcctccac ccacctggcg tgcagcgccg 33120 cgggcagtgg cacgtacacg gcctggatgt tgtcgcgtcg caggagctcg gcgtacccct 33180 ggaccgggtg gcagtcgaag cgttcggcga cccgttcggc cttggcgagg tcacggctgg 33240 cgaccgccgc gatctccagg ccgggggagg cggcgaaggc gggcatcatg cgccgcaccg 33300 cgatgtccgc gcagcccagc accccgatcc gcaccggggc cccggccacc gtgcccgcgc 33360 tcaacgtgcc ctcgccggga gtgcgttgag gcaggccagc agcgtgcgga cctgcacgtt 33420 gagatagtgg ccgtggcgga cgagccaggt gagctggccg ggggtgaccc atgtgtagcc 33480 gggcggtggg tcgagcggcg cgtcgtgctc ggcggcgtcg atgatcagat agcggctctc 33540 ggcgttgcgg aaccggccgc cctcctccga gtgcacggca ccgtagcgga cacggtcggg 33600 cgacgcgtcg aggaccgagt ccaggaaggg cgggcggtcc ttctccgcga ggtgggcgta 33660 gttgcccggc gtgtactgga cggtggggcc cagctccacg gtgtccagga agccgccctc 33720 gacccgggcg tgcaccagga cgtgcgggac gccgtcgaac tcctgggtga ggaaggcggt 33780 gacgccggtg ccgaccggct cgaacagggg ttgggtccag ctggtgacct cgcggttgcc 33840 ggcctggacg gccaccccga cgacgttgaa gtagcggccc tcctcgtgct cgacggtcat 33900 ctcgtcctgc cgccagccgg gcagtccggc cagcggtacg cgttcggcgt gcacgtcgtg 33960 ccgggcgcgc tccccggtga tccaggacag cagctcgatg tcggtgagca gtgcgccgga 34020 cgccgtcgcc ggcaggggca ggcaggacag cacggtccgt gagtccatgt tgacgacatt 34080 gtcctggtgc agcagctcgc cgatctgacc gagggtgagc cagcagaagt cgtcgaggag 34140 cgggacgtcg ccgaccgcct ccacgatcat gttgcggttg gacttccgga agaaccagga 34200 gccgtgctcg gactgcaggg cgtccgcgac gacccggccg cggccggggc cgacgaagtg 34260 ctcgatgtac ttcacgtccg cgccctggtg ggccttggtg tagttgctgc gggtggcctg 34320 gaccgtgggg gagagctgga gcagattcgg gttgccgggc tccatcttgg cctgcatcag 34380 gaagtgcggc accccgtcga actccttgac gaggatgccc aggatgccga cctcgggttg 34440 cttgatgatg ggctggtacc agtcggcgta cgggccgtcg ccgtaggggc cgtcccgctc 34500 ggtgacgtgc aggccctcga ccgtgaagaa ccggccgctg cggtgcacca ggtttccggt 34560 ggactcctcg aacgaccagc cctgcaactc ggcgaacggg atgcggtcca cgcggaacgc 34620 gttggcccgg ccgcgctcgg cgagccagcc gtggaagtcg gcggtcgcca gctgggaacc 34680 ctcggtggtg gcggccgaca gcgcgagccg gtcggccagg gcggcctcgc cgcgcgatcg 34740 aagggtgtgg gacagcatgg gagaactccc tcagtcgacg tcgacgatcg tgaacaggcc 34800 cttcttgacg agcttgatgc cgtcggtggc gtccttgttc caggtgatga gcgcataggg 34860 gcccgtcccg gtcgggacct tcaccaccgt cgaccggtcc gtggacagcg ggaggcttcc 34920 caggctgacc gaggtgaagg gcgagcccgg cgggaagctg ccgtcgtccg gcatcgagtc 34980 gaagtacgcc ttcaggtcgg cctcggtcgt cccctccgcg atccgtacga agaggaactc 35040 gttgacctgg cccgggatcc ggttcacgaa ccgcacgggc tgtccggccc gcaccttgcc 35100 ccggacctcg aagcggggac cggtgcccgg cacgttcttc gacacgaccg tcgcggtcgg 35160 cgtcagaccc tggtccacgg tgctgccgga caccgtcagc cagcggtagc ggggctgggc 35220 gtcggccacc gcgaggtagt cgaacagcag gtaggtgccc ggagagaggc gctgggtgaa 35280 ggagccgggc tgcccgtcgt ggatgaccag cccgccgagc agggtggcgt cccggttcag 35340 gtccttggcg ccctggggca cctcggcagg tgtgttgccc agcgcgcgga acaggtcgtg 35400 cgtgaagtcg tcccagctca ccccgggcgt cagccggacg aggccgatcc agcccttgcc 35460 ctgctgcgtg gtcgaggcat tgaaggtggt gagcccgggc cgggtggtcg ccggcgccgt 35520 gaacccctcg gcggtggagc gcagttcgac gctgtgggag ccctcggcgg ccgaggcggc 35580 gaccgggctg ctcccgacgg gaggggcagc gaccgcgggc ccctgcacaca cggccaggag 35640 cagcacgacg gcaccgccga gcccgagcgc cacccggcca ccgagcccga gcgcggtgcg 35700 cccaccgggt ccgcgagccg tgcgcccacg gagtccgcgg atcagcctgc cgccgggtcc 35760 gggcctggtc cggtccgccg tcctgccgtt cactgtcttc tcgttcatcg cgggtacgtc 35820 aacgcctttc acatgcgtgt cgggtgcggg ggcggagccg aagccgtgac tcagccgcgc 35880 gcggccacga actccttgat cgaggcggtc acgtagtcga gcatctcgtc ggtgagcgcc 35940 gggtagaccc cgatccagaa cgtgtgctcg gtgatgatgt cgctgttggt caggtcgccg 36000 accacacggt ggggctggtc gatgtaggcg ggatgccggg tgagattgcc ggcgaacagc 36060 cgccgggtgc cgatcttgcg gtcctccagg tgggacacca actccgcccg gccgaacggg 36 120 gcttcggggt ccacggtgat gacgaatccg aaccagctcg ggtcggagcg gggcgtggcc 36180 tcgggcagga tcagcccggg tacgccgtcc aggccctcgc gcagccgccg ccagttgcgc 36240 ttgcgggcgt cgatgaagtc gtcgagcttg gcgagctggg tcagaccgag ggcggcctgg 36300 atgtcggtgg ccttcaggtt gtaaccgacg tgcgagaaga tgtacttgtg gtcgtagccg 36360 gccggcaggg tgcccatctg gtacttgaac cgcttgaggc acttgtcgtt ctcgcccggc 36420 tcgcaccagc agtcccggcc ccagtcccgc agcgactcca cgatccgggc gagcgccagg 36480 ttcgaggtca gcacacagcc gccctcgccc atcgtgaggt ggtgcgccgg gtagaagctg 36540 accgtggtca tgtcgccgaa ggtgccggtg agctgtccgt cgtacagcga gccgaccgcg 36600 tcacagttgt cctcgatgag gaacaggtcg tgttcctcgg cgagttgggc gatcgcggtg 36660 acctcgaagg ggttgccgag cgcgtgcgcg atgatgatcg cccgggtctt gggcccgatg 36720 gcggcggcca ccttgtccgc ggtcgcgttg tacgtcgtca ggtccacatc gacgaagacc 36780 ggtacgagcc cgttctgcag gatcgggttg accgtggtgg ggaagcccgc cgcgacggtg 36840 atgatctcgt cgcccggcct cagccgccgg tcctccagat gggacgaggt gagcgccgag 36900 acggcgagga ggttcgccga ggagccggag ttggtgagat gggccttgcg ccgcttcatc 36960 cgccgggcga acgccgactc gaatttgcgc tggctgcgcc cggcggcgat ccgcatgtcg 37020 agggcggctt ccaccagcgc cacccggtcc tccacctcga ggaccgcgcc cgagggccag 37080 atctccgtcg tgccgggcac gaactcccgt gccggtgtgc tgacttcgtg gtacgcgcgg 37140 accgcgtcga gcaccgtttc cttggggtcg ctcatcatcc tgtcctttcg tcgacgggcg 37200 gcatcacggc cgcgagcagt tcgcgcaatg aggtgtccag cgggcggcgc ggcgtccagc 37260 cgagcagttc gcgcgccagg gagatgtcca gggactgcca ctcgacgtcg ttgcggttcg 37320 gcggtgcgcc gccctcctcg ctcaccggaa cctcgaggcc gctgagcgcg atcatccggt 37380 cgatcaggtc ccggaccggc acggcctcgc cgcggcccac gttgatcacg cgccccctga 37440 ctcgcgcgcc gggcgcgacg gcagcggcca ccacggcgtc cgccacgtcc cgtacgtcca 37500 ccaggtcgcg ggcggcccgc agcggcggca gccgcagctc gtccggcttc tcgccgaggg 37560 agtcggcgcg ggcggccccg gcgagatgcc gggcgacctg gccgaagaga ctggccacgg 37620 agatcccggg gccgatcaca ttggccagcc ggagcaccac cgcgtccgcg tcctgatccc 37680 gtacggcccg cgacacggcc tgggtgccga ggagtttggt acgcccgtac ggggtgacgg 37740 gcgccggcgg ccagtcctcg gcgatggctc ctcccggagt gcccgccccg tactcgtgga 37800 cgctgccgag ctggatcagc cggggtctgc gcggctgcca ggggagtgcg gcgagcgcct 37860 cggtgacctt ggtgaccagc tcggcgttgc tggcggccat ctgaggctcg tcggcctgcc 37920 agacccgccc cgcggagttg acgacggtgt cggcgccgac cgtggcgagc aggcccgcga 37980 tctcccgggg ggaggcggcg agcacgtcga ggcggacggt ggccgtggcg cccgagcggg 38040 gtacggcgct gggggagacc aggtgcaccc gggccccgag cgcggtgaag gcctcaccga 38100 tgtggcggcc gatgaacccg gtgccgccga gcacgacgac agtgccgccg gcggccttgt 38160 cgaggtcagg caacggcccg gtccgttccg tgcttggcgg ccttccacca gtccgggttg 38220 tcccggtacc aggcgaccgt ttcggcgaga ccctgttcga agccgatccg cggggcgtag 38280 cccagctcct cgcggatctt ggtctcctcg atggagtagc gcaggtcgtg cgccttgcgg 38340 tcgggaaccc gctggatcgc ctcggcgccc gcaccggtca gttcgaggag ctgctcggtg 38400 atggcgaggt tggtgcgctc gttgccgccg ccgatgttgt agatctcgcc cgcccggccc 38460 tggttcagga ccaggtggat gccccggcag tggtcgtcca catgcagcca ctcacggacg 38520 ttggctccct cgccgtacaa cgggacccgg cggccttcga gcaggttcgt gacgaacagc 38580 gggatgagct tctcggggtg ctggtacggc ccgtagttgt tggagcagcg cgtgatggac 38640 aggtcgacgc cgtggctccg ccagtaggcg cgggccacca ggtcggagcc tgccttcgac 38700 gccgcgtagg gcgagttggg cgcgagcggc cactcctccg tccaggagcc ctcgtcgatg 38760 gagccgtaca cctcgtccgt ggacacgtgc acgacccgct ccacgccgct gtcgagcacg 38820 gcgtcgagca gcgtctgggt gcccaggacg ttcgtacgga agaagtcgcc cgcgccttcc 38880 agggaacggt cgacgtgcgt ctcggcggcg aagtgcacca cggcgtcgtg gccgggcacc 38940 agctcgcgca gcagcttgcg gtcgcagacg tcgccgcgta cgaacaccag gcgctcgtgt 39000 gaggccggca ggttgtcgcg gttgccggcg tacgtcagct tgtccagggc ggtgacctgc 39060 gcgtcctccc acccgctgta cgagccctcc agcacgttgc gcacgaagtg ggagccgatg 39120 aagccggccg ctccggtgac gaggatcctc atgccgtgac ctccactcgg gtgtggtctc 39180 cgacgaccag ccggtaccgg tcgacgccct gctcactggt gccgaccgag gcggaccgcc 39240 cgatcagcga actgcgcagg cccttgacgc cgctgaccga ggcctcgtcg agggcgatgg 39300 agttctccac ggcgctgtcg ctgaccaggc agccgcgccc gatggacgtg tgcgggccga 39360 tgtggctgtt ctccaccacc gcgcccgctc cgatgatcac cggcccctcg atgcgcgacc 39420 gtgtcacgcg cgcccccgcc tcgacgacga ccgcgcccag cagctcgctg aggtcgtcga 39480 cgtcaccctc gacggacggc gccagccggt ccaggaggta gcggttgcag tccaggacgt 39540 cctcgacgtt cccggcgtcc ttccagtagc cgccgtactg ggtggcgcgg acctcggcgc 39600 cggaggccag cagccactgg atggcgtccg tgatctccag ctcgccacgg gcgctgggct 39660 cgatcgagtc caccgcccgg tggatggccg aggtgaagaa gtacacgccg accatcgcca 39720 tgtcgctgcg cggttcgcgc ggcttctcca ccagacgcag cacctcgccg tccggcccga 39780 gttcggcgac accgaactgg cgcgggtcgg tcaccttgtg gaccacgatc tgcgcggccg 39840 gacgccgggc cgtgaactcc tcggcggtgg cggcgacgcc ctcgggcagc acgttgtcgc 39900 ccaggtacat cacgaagtcg tcgtcgccga ggaagtcccg ggcgatggcc accgtgtggg 39960 cgaggccgcg cggggcgtcc tgggagatgt aggtgagttt cacgccgaaa cgggagccgt 40020 caccgagggc ggcgtcgatc tccgggcccc ggtggccgac gatgacgccg atgtcggtga 40080 cgcccaggtc ccggatgttc tccagaacat gtaccaggac gggggtgttg gcgatcggga 40140 tgagctgttt cggcatcgaa taactgaagg gtcgcaagcg ggtaccggaa ccgcctgcca 40200 gcacaagcgc cttcatgagg agtcctctcg attcacgtgt gcatgccttt tctcgtcgtg 40260 ctccgggcgt tcgtgcctcg ggcgtcgggg ccttggcggt tcagcggagc tgttccagtg 40320 ccgccaccac gtgggagggc agcggcatcg tggcgatctc cgcggagagt tcgcccgccc 40380 gctcccggta tccggggcgt gcgagcagtt cctcgcagga gtcggcgatg cgctcggcgg 40440 tgtcctcgcc gggcagcagg gtgatcgccg ccccccgctc ggcgatgcgc agggccgggc 40500 gttccatgac ggcccccttg gggatgagca gttgggggac gcccgcgttg agtgcggtca 40560 gggtgctgac accgcccgcg tggtggacga gcaggtcgca ggtgggcgcg atgacgtcga 40620 gcgggaccca gccggcccgc aggcccggca gttcggcggt gagggcatcg gccacctcct 40680 ggggggcggc gacgatgagt tcgacgtccc acgcggtgac gtccttggcc atgccgcgca 40740 ggaactcgaa gttccggtcg tagctgtcct tggcgacccg gctgcccgag gtcacacaga 40800 tccggcgccg ctcaccgcgc gagtacatcc agggttcgag cctgcgctgc ccgttggccg 40860 ggatgtagcg catcatctgc gcgggaccgg cgttcgccgg gcgcaggctc ggcgggcaga 40920 tgtcgacgaa cagctcgggc tcgggcagcc ggtcgagccc caactcggcc agctccggcg 40980 ccagttcggt gtcggcgccc ggatggatgc cgtcggcctc gatggcgtcc caggcctggc 41040 gcacatgcgg gatgcccaga tgcgcggcga gcagcggggc gacgtagcac atcgtgccgc 41100 cgacgacgag gtccggccgc caggaccgcg cgaactccag catgcgcggg aggctctccg 41160 cgcccatgcg ggcgaaccag cggccggtga acagcgcctg ctcgacgggg tcggagggga 41220 tcgactcggg gttgccctgt cggtcctggg tgatgaactc ccggatcggc cggtgggtgg 41280 tcgggatgcc ggccagtccc gagcccgtga tgtgcggcat catgtccgcg ttcgcggcca 41340 tcacgacctg gtgcccggcg ttgcgggcgg cggtggccag cggggcgagc gcgaagacgg 41400 tcgcggggct gcccgcggtg acgaagagga tcctcacagt gttcctgcct tggtgaatcg 41460 gggcgtgcct gtcccgccga accggggcag aacgccggcg gctctggcct cggccacggt 41520 cggtgccttg gcgtccttgt cggagatgag ggggacttcg gggcagtccc acggcagatc 41580 gagctcgggg tcgagtggat tgatgtcgat ctgcgttccg ggcacatagg tgctggagac 41640 gacgtagcag atgcaggcgt cgtcggtgag cgcgagaaag ccgtggccga cgccctcggg 41700 cacatagacg gatctgcccg aatccgcgtc gagttcgttg acctggtgct tcatgaacgc 41760 cggggaaccg atccgcagat cgaccacgat gtcgcgcagc acgccccgca cacaggtgac 41820 gtacttcgcc tgacccggcg gaatgctcac gctgtggatg ccgcgcagcg tatggcgtct 41880 cgacaccgag tagttgatct ggcggggctg gaaggcgaca ccggtcgcct tctccagcgt 41940 gtcgtcacgc atcgcctcgt agaagttccc ccgctcatcg tgtatctgac ggggcgtcac 42000 cagatatgta ccgggaatga ccatttcctc gatcagcacc gcgcgttcgc ctctctcggc 42060 tcagtggcct cggggtggtg gcctttcatg cctgacgggc cagctgttcc aggtcggccg 42120 cgacatcggc gggcgacggc atcgcggcca tctccgcggc gagccgccgg gccgccgcgc 42180 cgtacgcggg atccgacagc agggtgtcca gggctccggc gatcagctgc gggtcgtcct 42240 gctcggctgc cttgtcgaag gtgaggcccg cgccggtcgc ggcgagccgg tcgcccagcg 42300 ggaagtggtc cgccagctgg ggcagggtga gctgcggcag cccgaaggcg gtcgcggtca 42360 tcgcggtgcc cgcgccgccg tggtggacca tcgcgtcgca gcggtcgagg aacaggtgca 42420 ggggcagcgg atcgatcagc cgtacgtgtt ccggcacggc gccgataccg gcccggtgcc 42480 gctcgggcac cgtcgcgagg atctcggtgc cgggccggcc cgcgagggca tgcagcaggt 42540 cccgggtgaa cggttcgccg tgcagggcga gggtgttgcc cagcgagacg gcgacccgct 42600 gccgtccggc accgggcccc cggtccgcgc gcagccagcc gggcacctca ccaccgccgc 42660 tgtagggcac gtaccggatg gacgtgcccg gctcggagtc cggcagccgc agggacggcg 42720 ggcacgggtc gagcagcacc gccggatccg gcaggccggg caggcccagc tcctcgcaca 42780 gctcccggaa gtcggggcgc accaggcgcc gggccggacc ggagatggcg tccaccgtcc 42840 aacggtggtg caccaccggc acgcccagcc gcgcgccgag gatcagcgag cagtactcca 42900 gcgggtccga gaggatcaac tcgggcccga actcccggac gagctcgccg tagtggccga 42960 gtacggactt ggcgtgcccc atccagagct tgccgtatcc gccgagcacc tcgggcgcgg 43020 gccggggccg cgcctgcaac ggccgttcct cctcggccag tccccgcagc agcatcccgt 43080 ccacgtcgaa cgactcgccc acgaccgcgc cggtcagccc ggcggcccgc acggcgccga 43140 gcacatcggg ctgcccggtc accacgacct cgtgccccgc cgagcgcagc gcccagacga 43200 gcggcacgag cggcaggaag tgggtgggaa caggggtgca gatcacgagt acgcgcatct 43260 gacggggtcc tctcggtggc tggtcggtgg gtcgccgtca gcggcgctcg gcggtcagac 43320 gttccagcag cggtacgacg tccgtgggcg ccggcgcggc gagcatctcc tcgtacaggc 43380 cgcgcgcgcc gagctggaac gacgggtcct cgaggagtcg tacgagctgc ttgtgcaggc 43440 cgtcgacgtc cagcgcggcg gggtcggcga ccaggcccgc gcccatgtcg gcgacatagc 43500 gcgccgtgac catctcgtcc cacatgacca gcggcaccac gagctgcggc acccggtggg 43560 ccacggccgc ggcgaaggtg ccgccgcctc cgtggtggat gatcgccgaa ctggtcggca 43620 gaacctggtt gagcggcacg tactcgacgg tgcgcacgtt gtcgggcacc ttgccgaccg 43680 ccgcgagctg ctcgttgttg agggtggcga cgacctcgac gtccagggcg gacacggatt 43740 cgaagaactc gcgcatcggg aagccgctgt acttgccgaa gatcttgcgc cggctcaccc 43800 ccagggtcag caccgcgcgg ggccgctcgg gccgcgcgtg cagccagtcg ggcagtgcgg 43860 ccgccccggt gtaggggacc cggcggaccg ggatgtgtgt caggtccagg ggatgccgca 43920 tccgggactg ggtcaggtcg agggtccact ggccgagcag catctcgtcg tcgagctcca 43980 gcccgtaccg ctccagcatc gggccgaacc agctcagcca cggatgctcg gtcaactccg 44040 agcgcgggtc cgccagttcg cgcttcgtgc ggtcgtggat cacggcgacg ttgtccatgc 44100 cccagaccag ccgggcgtgc gcggcgccgc tgacccgcgc ggcgatcggg gccgcgagca 44160 tcagggggtc ccacaggacc agatcggggc gccaggcgcg ggcgaagtcg accaggttgt 44220 ccaggaccgg gcggcgtccg ccctggcgct cgggcgccgg gtagtacagc gagaacatgc 44280 tggcgaggat ggagcgcgcc gtgcgccagc cgccgctctc ctccggctcg ttggggtcga 44340 agcccagcga cggacggtcc ggcttggcgt ccccggtgtg cgcaccgagc gccggggaca 44400 gctcgtccgg ggtgccgagc gacaccgcgg tgagcccggc cgcgccgatg ttcccgatca 44460 cccccgggtc caccacgccc gagtggctcg ccacgacgac ctcgtgcccg gcgctctgca 44520 gcgcccaggc aagcggcacg accgagtaca ggtgtgacgt cgcgggaaag atggtgagca 44580 ggacacgcat cgttgatctc cgttcgcgtt cgggagcggt cgtcaggtcg ccgtgtgcgc 44640 caggggagtt cgggtccaca ccgcggtgag cgcgccggcc gccgacaggg cggtgaggaa 44700 gagcagcgcc ggtcggtatc cggcaccggc cagcacggcc acggtcggca gggcgagtac 44760 cgcgccgagt tgtacggcgg tctggtagac cggcaccgcc tggcccttcc ggtcggccgg 44820 aatcccggac atggcctgca tgttgagagc ggcgaacgac agcacgaagg ccgcgccgac 44880 cagcagcagc gtcggcagca ccccggtgat gtacgagtcc ggctccccgc ccgccagcgc 44940 ggtcacggag ccgagggtcg ccgcgcagcc cccggcgacg atcagccgct cgctcccgaa 45000 acgccccacc agccgcccgg cgaagggcag cgacagcgcg agcggcacgc aggccggcag 45060 gaacgcgagc gccaaccgcc aggcgtccca gccccattgg gcgtgcaact ggtgggtgag 45120 gagcaggagc agaccgagat acgtgccgtt cagcgtcgcc gcgcagaccg cggagcggac 45180 gaaaggaccg ttgcgccaca gccgtacgag cgtctcccga agcggcggcg gaccggcctg 45240 cgacgccgcg tccgcgggaa tcagccggaa gccgaacacc agcaggagca gggcgatcgg 45300 cgctgggaag acgagatccc accgccagct cagcgcggtg agcgcccccg aggacagcag 45360 cccgaccgtg aacccggcgg caccgaacag cgagtacacc gacacggccc ggcgctgttc 45420 cgcacctgcc cggaaggtcg taccgatgat cgcgagcccc gtcggtgcgg tgagcgcggc 45480 gcacatgccc ttgacgaccc gggtcaggat cagcagcgcg ccctgatcgg ccaggccacc 45540 ggccaccgag gcggccacga agaccaggag cgccgccaga tacacgcgtc gtctgcccca 45600 gcgggcggtc acgcgcggac cgagcagcag cagggccgcg aaaccggccg cgaagccgct 45660 catcacccac tgggtgcccc acatcgagag ccccagatcg tccccgaggg ccggcagcgc 45720 gaccagcacc acggacactt cgagcgcgtc gatgagcatg ttcccggcga gcacgaacag 45780 aagggcccgg tgggcgcggc tccccttcac cgaaggggca ccggggcgtg gagagactgc 45840 cgtcatgacg tgctgaccac cttcggggcg acaggaatcc gtgcacaggc cggccgtggc 45900 tcatcggggc atcgaccgag ccacggccgg ccagtcgggg ggcgggccga ggggggcggc 45960 ccgcccggtc agcgcagcag cgtgccgccg ctggcgtcga ggtaggagcc ggtgatccag 46020 cgggcgtcgt cgctcgccag gaacgccacg acatcggcga tgtccttggt ctcgccgaca 46080 cgctcgaaga ccgagtagcg cgccatctcg gcgacggcct ccgggttgtc gaagaccggg 46140 gtgccgttgt ccgtgatgcc gggccccacg ctgttgacgg tgatgttccg cgggcccagg 46200 tgcttggcgt agtgcagggt cagctggtcg atggcgccct tggtcatcgc gtaggccacc 46260 tcctgcgggt tggcgaaccg ggtcagcccg gaggagatgt tgatgatgcg tccgccgtcg 46320 ggcaggagcc gcagggcccg ctgcacgatg aagaacggcg ccttggcgtt gacggcgaac 46380 agccggtcga actgctcggg cgtgacgtcc tcgggtgcca ccccgcccat cacccccgcg 46440 ttgttcacga cgatgtcgag agtggtctcg ccggtgcgct ccttcagccc gcgctccaac 46500 tccaggaaga gcgcgtgcac atcgccggac acgccgagtt cggccgccac ggcgaacgcc 46560 cggccgccct ccttctcgac ggcggccacg gtgtcgtcgg ctccctcggt gtcgcgggag 46620 cagtgcaccg cgaccagcgc gccctcgcgg gccagacgga tcgccgtcgc ccgtccgatg 46680 ccgcggctgg acccggtgac gagcgcggtc ctgccggtga gcttgcccat ggtgctcctc 46740 ctgtgcgtgc gggtgaacgg gcctgtggcc gtggggtgtt ctgtcctgcc ggtgtccgag 46800 acggcggggc gcgggccgtc gagcccgctg atcatcgagg cgaggtggcg gcgggcgggt 46860 tcgagcgcga gcagttcccc gaacaccgcg cgcagggcgt ccacttcggg tccgccgaac 46920 agcagctggg cctgcgcctc gatgttgccg agcacccgtg cgcccaccgg gtgccgctcg 46980 gcgtggtagc tgtcgagcat ccggtcgtcc gcgcggccgg tgatccgcgc cgcgagtttc 47040 ccgccgagcg cggccgcgtc ctggagaccg aggttgaggg cctggccgcc gaccggcagc 47100 tggactgcgc ggcgtccccg gcgaggagga cccggctgtg ccggtaactc gccgcctgcc 47160 tgcgggcgtt gtggaaggcg ttgacccaga cgggccgggc gccggtgatg tcctcgccgg 47220 tcacccgcgc ccagacccgg cagacctcgg cgaagtccgg ggcccggccg cccggggcgg 47280 ggtgcccggc cgtactggtg aaccatgagg cgggtgacgc cgtccggccc gcgccggcag 47340 ttggccaccc cgttcggatg ccgctggaag cgccggtccg ggatgtcgat tccgctgatg 47400 tccgcccgca gcagctcctt gaccggggcg ctgccgggga actcgaaacc tccgaggcgc 47460 cgtacggtgc tctgctcgcc gtcacagccc gcgaggtaag ccgctccgaa ccgcagccgc 47520 tcgccgtcgg gaccggtgac agcgacgtcg acccggtgct ccgcgaccgt cagcccggtc 47580 accgtatgcc tgcgccgcac ctcggcaccg agccccgcgg cccactcgta cagcaccttc 47640 tcgaccagtg tctgcggtgc cttccactgg ccgggaaacc ggctcgggtg cgccgcggcc 47700 aggtcgagcg ggatgccgcc gaagtggccg gggcccgcgc tcggcggtgg cccgagccgg 47760 tccagcaggc ccagaccgtc gaggatctcc atggtcctgg agtgcagtac cgacgcccgt 47820 gactcgtcgg tgggctcggc cagttgctcc agtacggtca cccgggcgcc accgaggcgc 47880 aggatcccgg cgagcaggag acccacgggc cccgcgccca cgacgaccac gtcggtgtcc 47940 ggtgcttcgg caacctgtac ggccactgct cagcgccgcg cttcggcgta cgccttggcg 48000 tggccgagcg tcgcccggct gttgttgccc agcgcgttgc ggatgaactc gcgggcctcc 48060 ggaatcccgg cctcctcgcc caggatcttg gtgatgttct ccgtgttgag gatcaccgtg 48 120 tgctgcgagg tggcggtggt gacgccgtcg gccgactcgt cgagctgcca gtaaccggtg 48180 tgcaggttca tcagcgcggg cagcgtcgtc tgcttgtaga cgatccggga gtgcgggaag 48240 cacacccgga tcgactcggt ggtgtggacc gagccgtcct tggtcagcgt gtccatccgc 48300 aggacctgca ggccgggcga gtcctcggtc agcgagacct gcttgacgtg cggcagccgc 48360 tcgctccaca gctgggcctc gttgatgaag tcgtagacgt ccttggcggc gcccttgatc 48420 tccagactgt cggtgaacga cagggtcagc tcggcggagc cggtgacgcg ttcgacgttg 48480 cccttcagcg cggccagctc ggaccggctg ttgcggtcga cggcctggtc gatccactcc 48540 aggccctcgg gatcgtcgtc gacggcccgg tagtcgtgca gcaggcgcac gcgggacgcg 48600 ccctcgccgg ccggctcgat gatccacgtg ccgcccatcg acgccaccgg cggggcggac 48660 acctcctgcc ggaacgtgat gcgccgggcc tcggggtcga gcgtgcggcg cgaagtccag 48720 cccttggccg cgccgttggc ggtggcccag atgcggatgc gctcctcgcg cgcgccgcgt 48780 tcgaggtggt cgacgtggac ggtcggcggg aagatcagcg gccagttccc gacctccgcg 48840 atcagccggt acacctcggc ggccggggct tcgacagtga tctcgtgctc gacctcacgg 48900 acggtcatgg tgaactctcc tcacggataa gggatcagaa attgccgagg ccgccgcaga 48960 cgttcagggc ctgcgaggtg atggaggcgg cggtgtccga ggcgaggtag ccgaccaggc 49020 cggccacctc gtcgggcgtg gagtagcggc cgagcgggat cttcgcctgg aacttctgca 49080 ggatcgcgtc ctcggaggtg tcgtacgcgg ccgcatagcc ctgccgtacg cgctgggcca 49140 tcggcgtctc gacatagccg gggcacacgg cgttgacggt gatgccggtc ggggccagtt 49200 cgttgccgag ggctttggtg aagccgacga cgccgtgctt ggaggccgag tagggggcgc 49260 cgagcacgac accctgcttt cctgccgtgg aggcgatgtt gatgatccgg ccgcgctcct 49320 tggccgccat gccgcccgtg ttgagcacct cgcggctcat caggaacacg ctgtgcaggt 49380 tcgtggcgat gacgtcgtac cagagttcgt cggcgatgcc ggcggtggga ccgccgccgc 49440 tgcggcccgc gttgttgacc aggacgtcga tggggccgaa gcgctcgacg gcctgccgca 49500 cgaagcgctt cacgtcctcg ccctcgcgta cgtcgcagac ggcgccgtcg acgtcgaggc 49560 cctcgtcgag cagctccttg accgtgcggg tgacaccgtc ctcggtgcgg gcgcacagga 49620 acacccggtg accctgctcg gcgaggagcc gggtcaccgc gaggccgata ccgctggtcg 49680 cgccggtgac gacggcgacc ttcttctccg tgttgccgga cgtggcagtc gtcatgtctg 49740 ttgtctcctc tatctcctga cggggcgtca gctggaccgg acgggcacca ggaaggtgtt 49800 gacggcgccg atcagcgcgc gaggtgtctt cgcctccgtc accgtgtcgt cgtcgagcgt 49860 gatcccgaac tcgcgctcga tccggccgct ggtctccagc agggccaggg actcgtatcc 49920 gagagactcg aagtccgtgt cgaggatgtc gccgtccagg tcgacggcct catcggtgcc 49980 ggccccgtcg cgcagaatgc gccgaaggtc ttcgatggtg aactcgtgag cgggcatgac 50040 ggtcctttcg tcgggcggtt cgaggagaga gagcgaggcg gacgggggcg cagcggtcac 50 100 ttgtcgacgg cccgtaccac cacggccgag ttgaagccgc cctgcccgcg ggcgaggacc 50160 agggccgagc ggaccgcggc gtcgcgcggg cggtcggtga ccaggtcgag ggcgtaggcg 50220 tcgctgagac gggtgccgac cgaggccggg atcacgccgt cccggatggc gaggaacgcg 50280 gcggccagat ccagcggggc cgccccggag tacagccgtc cggtcatcgt cttgggcacg 50340 ctgaccggga ccccgcgggc cccgaagaca ccggtgatcg cgtcggcctc gatgcggtcg 50400 agctcgggca gggcggcacc gtcggcgaac accacgtcga tgtcgccggc actcgactcc 50460 gcgtcggcca gcgcgagttc gatcgccttg cgcaggcccg gaggacgtcc gccgccgggc 50520 cgggggtcga aggtggagcc gtaccccgcg atctccccgt agatctgccg ggctccacgc 50580 tcgcgggccc gctccaggtc ctccaggacg agcagcgcgc cgccctcgcc cgggacatgc 50640 ccgcgcgccg ccgcgtcgaa gggcacgtac gcccgcgccg ggtcgtcgct ggtgctcagc 50700 cggtccccgg ccatctgggc gacccagccc caggggcaga tcgaggcgtc gatcccgccg 50760 gacaccacca gcggcgtgcc cttgcggatc tgccgccggg cctgtgcgac cgcgtcgagg 50820 ccgcccgcct ggtcgccgac gaccaccccg ctggggccac gcattccgtt gcggatggag 50880 atctggccgc tgttgacggc gtagaaccag gcgaacgact ggtaggcgct gacgtaccga 50940 ctgccctggc tccagagcgc cttgagttcc ttctggccga actcgaagcc gcccgaggag 51000 ctcgccgtga tgacacccat gtcgtactcg gacagctcgg cgggatcgac gcccgcgtcg 51060 gcgaacgccc agtccgtccc ggccagggcg acctgtgtca tgcggtcggt ctgcggcagc 51120 agccgggccg ggagatgctc aaggccgctg aactccttga tctcgcccgc cagtcgggcc 51180 gggtactgcg cggggtcgaa gcgggtgacc cggccgatgc cgctggcgcc ggcccgggtc 51240 gccgcccagt acgcctccac gccgaggccg ttgggcgcgg cgacaccgag cccggtgacg 51300 gccacccgta cggcggcgct catgcggcgc tcctttcggc ccggtccggg cgggccagca 51360 cgatggcgct ctggaagccg ccgaagccgc tgccgacgga gagcaccgcg tcggtctgcc 51420 agtcgcgtgc cgtgagcggc acatagtcga gatcgcactc ggggtcgggg gtgtgcagat 51480 tggcggtggg cggtacggcg ccgtgttcca tggccatcgc ggacgcggcg atctcgatcg 51540 agccgatcgc gccgagcgag tgcccgacca tcgacttgat ggagctgacc ggtacgtcgt 51600 aggcacggct gcccaggctg tgcttgaacg cggcggtctc gtgccggtcg ttctgcttgg 51660 tgccggagcc gtgcgcgttg atgtagtcga tgtcctcggg gttcagccgg gcctcgtcca 51720 tggccacccg gatggcctcc gccatctccc gcccgtcggg ccgcagtccg gtcatgtgga 51780 aggcgttgct gcgggtcgcg tacccggcga tctccgcgta gatgtgcgcg ccgcgggccc 51840 gggcggcgcc cagctcctcc aggacgaaca cggcggcacc ctcgcccagg acgaacccgt 51900 tgcgggtgcc gtcgaagggc cgtgaggcgt gctccgggtc gtcgttgcgc ggggtggtcg 51960 ccttgatcgc gtcgaaacag gccaccgtga tcggcgagat gggggcgtcc gcggcaccgg 52020 ccaccatgac gtcggcggcg ccctcccgga tcagctcggc ggcgtatccc accgagtcga 52080 ggcccgaggt gcagcccgtg gagaccaccg tgctcggacc ctcggcgccg accgcccagg 52140 ccacctcggc ggcgaacgag ctgggcacca ggtagttgta gaggtggggc acggcgtagg 52200 tgtggtcgac caggtcgagc cgcccgttgt cgctgacggt ccggtactcc tcgtcgagcc 52260 ccatggtcgc gccgaccgcg ctgccgatgg tcaccccggt ccggtgcggg tcgaggccct 52320 cgaactccag cccgctgtcg gcgaccgcct cgcgcgccgt gacgaccgcg aactgcgcgg 52380 cccggtccat gcgccggatc tcctgcgggc cgagccccag cagcgccgcg tcgaagtcgg 52440 cctccgcggc cacctgggag cggaacggcg acggatcgaa gaagctgata cgccgggtgg 52500 cggtgcgccc gtcggtcaac aggctccaga agttcttggc gcccacgccg cccggtgcgg 52560 tcaccccgat gccggtgatc acgacccgcc ggccgccggt cgcgctcaca gtgcggtcca 52620 gtggtagaag cgctgggcca tcgcgtcggc gggggagcgc caggtcgccg ggtcgtacgc 52680 ctcgatgaac ggcttgaggt cgtcgctgat cccgacgaac cgggggtccg tcttggcctg 52740 ctcgatccgc tcgccgccgt cctcgttgtc gaagtcctgc agatggaagt agagaccccg 52800 gtacgagaag agctggcggc gccgggtgcc catgaggtgc ggcatctcgg tgtcgtcgaa 52860 cctgttgaac agacgcgcga cgtcgtcggc ggatccgggc tccattcgag ccacgatcag 52920 cgtgctgtgc atctctctct ccttgtcagg acgatcggcg ccgggacggc cttgtgagca 52980 cggccggcgt caggacttcg ggccccgcga cgggccgaac cagcgctcca gcaccatcgg 53040 caggtcgtga tggctgccgg gcgcggccca cgccacatgc ccgtcggggc gcacgagcac 53100 ggctgaggtg ccggccagcg ggctgtcgtc ggacaggccg tgcggtgcgg cggtgacgat 53160 gtccacacgg tccgaccagc cggacgcccg gtcgcgcagg acagcgttgt cctccaggtc 53220 caggagcacg ccccgggccg cgcgcagcag ttcggtgctg ctggtcttgc gccggtcgcc 53280 gaccagctcc aggtgcggca tacgcaggcc gagcagggga tggcggccgc ctccgacgtc 53340 gtaccggatc tccaggccgc tcaccatgcc ggcgagatgg cggctgacct cctcgtaccg 53400 gatcagctcg cggagcacgt cccgcagcgg ctgcacctcg tcgccgctca ggaagagcag 53460 gccctgcgcc ttggtgttca tcatcaggcg ctgtccgacg gggtaccgct cgccgtggta 53520 ggtgtccagc agctcttcgg gcgcggtgcc ccggaccacg gcggccagct tccagccgag 53580 gttggcggcg tcctggacgc cggtgttcat gccctgtccg ccggccggga gatggatgtg 53640 cgcggcgtcg cccgccagca ggacccggcc gcgccggtac tcggtgacca ggcgggtcgc 53700 gtccccgaag gcgctgaccc acaccggctc ggcgtgcgag atgtcgatcc cggtgagccg 53760 ctgccaggcc gccgcgactt cctcgtacga cgggggctcg gtgcgccgct tgggcggggt 53820 gccccgctcg cagacgatga tgcgggtgat cccgccgtcg ccgagcggcc cgaccatgac 53880 catgccgccg tccacggtct ccccgatgag ccggggttcg agctcgacgc ccttgatgtc 53940 cgcgaggtac atctccatgg tcgccgccgt gccggggaag tcgaagccga cggccttgcg 54000 gacagtgctg cgcccgccgt cgcaccccac gagatagtgc gcggtcagcc gctgttcgcc 54060 gtccggcccg cgtacgtcga cctccacacc gtcggtggtg tcgcgcacgg cgagcagttc 54120 gtggccgcgg cggatgtccg tgcccagttc accggcccag ccttcgagca tggtctcggt 54180 ggtcgactgc ggcacggtct tggccgcctg gtgcacgctg tccagtacgg cgaagtcgac 54240 cggcagtcct ccgaagtgtc cggcgttgct ggtccccatg tcaccgaagc ggggcagcag 54300 cccgcgttgg tcgaagacct ccatcgtgcg tgtggtgaaa ccgaggccac gcgactcgcc 54360 ggtccgctcg gcgagccggt cgacgacgat gacgtcgacc cccgccagac gcagctcggc 54420 ggcgagcgtc aggccggtgg gccccgcgcc ggcgactatg accgaagcat ccatgtactt 54480 gtcctccggg catttcagag aggaattgca ggagaggggt tcggaatgta actggagtgt 54540 taattcagaa ccgaaataac tgaaccggca ttaattggcc gggaaacgct ttgcaccggt 54600 tggtcgacac agtgctgcgg aaactagtga ttagcttctt cgggaatcat cggaatgtca 54660 agaggagttt ttcaggcggg ccacaacatt ccgtcaagta attgttaagt cgcacttgag 54720 gatggtgagc catcgttttt ggaaggcgcg cttccggaac gcgcgccgag tgtccgctgg 54780 atgtcctcgc agtggtagta ggtgcgcccg ccctccgcgc gccttcgcca ccgctgctcg 54840 ctggcgtgcc ggtacacact gccgggcggg atgttccaca gccgggcgat gtcggcggcg 54900 ccgacccagt gcacacgggt gtcacgcgcc agcaaccggc tcaggctcag ccactcgtcg 54960 gcggaccagc ggtgggcggg tgaggcctcg cagcggatct ccagtggggt gtcggcccgg 55020 tgcggccgta cgagcgccac caggccgccc tggcagtcgg cctcgacgca ggacccgatg 55080 ggcacccggc gcagtgggct cgggtcgatc acgcgacgcg cgtcacgcac caacagggcc 55140 gtttcctggg acagttcagg ggcggcctga tgtgcggtga gccagtcgca gtgccgggcc 55200 aggaacgccg cgagggtttc cacgttcgtc ggcggagcgc tggtccggcg ctcctcgacg 55260 accacggacg accaggaccg cagcaccccg gcgatggccg accggacgtc cgccgcggcg 55320 gtgttgaagg gcaggcccgg caggggcccg cccgaggtgc gctcgcgcgg cccgtcggag 55380 ccgccgagaa gccggccgca ctcctcgtgg agccggggca gctgccgcag ttccgcgatc 55440 aggcggtccc ggcagtccgg gcagagccgg tgcccccgtg cggcgagcct gcggggccgg 55500 ccctcggccg cccgccgccc gcaccgtgca ccccggcaca cggttatttc ctgggtcctg 55560 cctgcgagta ctcgattcat ttcccccacc agttctcggt gaggtgaggc tatttccggg 55620 cctccggcac ggccgtgagt gaactcacct tctgtggggt gcgtgccgga gatctcttac 55680 ttctctgatg aatttgtcgt ggatttgaac gtgacgaaaa ttcagatgcc caggcgtgcg 55740 gagatcccgt cgaggaccga ctcgaggccc gtctcgaact gccactccag gtcgtccttg 55800 cggctcgcct cgcggaggta gcgctcgtac gtcgggtaac ggtcggtgcc catcagccat 55860 gtcatgcgtg gggccagacc ggtgcgcgcc tcgttcgcgc tggaccagcc ctgctcccgc 55920 atcagctcgt tgaggccgat ctcgaagccc acggcgccct gcacatagga gtcgacggtg 55980 cggaagaccg ccatcatcgt gtcggcgtcc aggtcgagcc cgtcgagcgc ggccagggcc 56040 cgctcgggca cggccagctg gttcggggtc agtgtgaaca ccgccgtgcg ggccacccac 56 100 gagtgcctga ggagcacggt cctggtgcgc agggcgagcg cccgcatggc gttccgccag 56160 tccttggcgt ccccgggcag cgacagctcg ccgtagacgt ggtcgaccat cagctccagc 56220 agctcgtcct tgccggagac atagcggtac gccgccatcg gcgcggcccc caggtcggcc 56 280 gcgaggcgcc gcatggtgac ggcgtcgagg ccttccgtgt cggcgatcgc cacggcggcc 56340 gcggcgatgc gctgcggggt gagcgtggag cgaggggcgg gtgcggggcg gttgaggcgt 56400 tcccagagcg agacggtctc cctgggctcg gacggctccg gcgtcacctg cttctcctct 56460 tttctccgag tggccatggg cggggttcct tccgggtgcc ggggttgtgt acaacgtaac 56520 agcagtagac aacgtacaca tcgatgtgta cgttgtatct gtagcgatac gccgtacact 56580 tccaggagtt ccgcatgagt gacctgaacg acagccctct gcaggtggcg gccctcgtcg 56640 gcagcacgag gggcggacgc ttcggtccgg tcgtggccga ctggctgctg agccagatcg 56700 cccagcgcaa ggacatggag gtcgacctcg tcgacctggt cgagacgccc ctgccgaccg 56760 tcatgccggc tttcggggag caacttccgg acgaggacat ggctctgcta ggcgcggtcg 56820 ccccgcgcat ggagtcggcc gacgccttca tcatcatcac gccggagtac aaccacagtt 56880 atccggcggc cctgaagaac gcgatcgact ggcacaaccc gcagtggcac gccaagcccg 56940 tcgccttcgt ctcctacggc ggcatctccg gcggcctgcg cgccgtcgag cacctgcggc 57000 tggtcctcgc cgagctgaac gccatgacga tccgcaacac cgtcagcttc cacaactacg 57060 gcgacaagtt cgactcggac ggcaagcccc tcgacccgga ctgtgacgtc gcggccaagg 57120 ccctgctcga ccaactggcc tggtggggag aggcgcttcg ggacgcccgc accgctcgtc 57180 cgtacaccgg ctgagacgaa agacggccat gaccagcgac acgacggaga ccggcggcac 57240 cgcggacacg gctccgcaga ccgccgggaa gctgccctac ctcggcatct tcggactcat 57300 gctcggcatc ttcctggcca ccctcgacgg acagatcgtc agcatcgccc tgccgaccat 57360 cgtcggtgac ctcggcggcc tcgaccactt ctcctggtcc gtcacggcct acctgctcac 57420 gatggcggcc gccactccga tctggggcaa gctcggcgac ctcaagggcc gcaagggcgc 57480 ctacatgtgg tccgtgctgc tcttcctggt cggcaccgtc ctgtgcggac tcgcccagga 57540 catgccacag ctcatcgcct tccgcgccgt ccagggcctc ggcgccggcg gactgatggt 57600 cggcgcgctg tcggtcatcg gcgtgctggt tccggcgcac gagcgcggcc gcgtccagtc 57660 gatggtcggc gtgatgctgc cggtcgcctt cgtcggcggg ccgctgctcg gcggcttcat 57720 caccgacacc ctcgactggc gctgggtgtt ctacgtcaac gtgcccttcg gtgtcctcgc 57780 gctgttcgcg gtcgccagag gcgtacggct gccgaaggag cgttccggcg gacggatcga 57840 ctaccccggt acggcgctgc tgaccaccgc catcctctcg ctcaccctgc tggccacctg 57900 ggccggcacc cgctacgact ggggatcggc acagatcgtg ctgctcgccg tcgtcgctgc 57960 cgtggccgtc gccgcattcg tccgggtcga acagcgggtg gcggaacccg tcatcccgcc 58020 gcggctgttc cgcagccgtg acttcgccct cgcccagacg ctcagcttcc tggtcggagc 58080 cgtactggtc tcggtcatca actacctgcc ccagtacatg cagttcgtgc agggctccac 58140 ctccaccgta agcggcatgc agatcctgcc cctgatgatg ggcatgctcg cggcgcagct 58200 gaccaccggg cggctgatgg accgcggcgg cctggagcgg gtggtgccga tcaccggggg 58260 cgccgtcacg gtcgtcggcg cgctgctgct gctcctgctg gacacggaca ccccggtgtt 58320 cgtcgcctcg gccctcactc tggtgctcgg catcggcgtc ggcatcctga tgcagagcac 58380 gctgctcacc acgatgaaca cggcaccgcc gcgcgacatg ggtgccgcca ccggaacggt 58440 cacgctactg cgcacgatcg gcggttcact cggcgtcgcc gcgctcggcg ccgtgcacct 58500 gggccgtatg acggacgagc tcaccggccg gctgggcgag cgcggcgccg aacagctcac 58560 ctccggcggc gagttgaccc cgtcttcggt cgacgggatg tccggtcgag tgcggggtgt 58620 cgtccaggag gccttcacca gcggactgca cggcgtcctc atcggcaccg ccctgctctc 58680 ggccgtcgcc ttcgccctcg cctggttcgt ccgtgggacc ccggccccca agacccccga 58740 acccgccggg gattcgcggc ccgcccaggt ccccgcgacg accacggact gacaccccac 58800 gttctgtctc ccagccctcc ttctttccca ccgattccgt caggcgcccc ggcgccccga 58860 ccgaaagcag acccttctca tgtcgaccac ctccgccgag cgcgccaagg aagtcctgct 58920 gcgccccttc accctgggca gtctcactct tgccaaccgg atcgtgatgg caccgatgac 58980 ccgggagttc tcgccgggtg gcgtaccggg cgacgacgtg gcggcgtact acgcgcggcg 59040 cgcggcgggc ggcgccggcc tcgtcatcac cgaggggacg tacatcggcc acgactcggc 59100 cggtaccagc gaccgcgtcc cccacatcca cggcgagcag gccctggcag gctgggccaa 59160 ggtgacgaag gcggtgcaca acgcgggcgg gaagatcctg tcgcagctgt ggcacgtggg 59220 actggaccgg aaggccggct ccccgcctgt acccgaggcc ccgagcatcg gcccctccgg 59280 catcgctgtc gacggcacgc ccgtcggccg cgccatgacg cagaccgaca tcgacgatgt 59340 cgtcgccgcc tacgccgcgg cggcagcggc cgccgagcgg accggcttcg acggcatcga 59400 ggtgcacgcg gcccacggct atctgatcga ccagttcctc tgggagcaga ccaaccgccg 59460 caccgacgcg tacggcggtg acctgggtgc ccgtacccgg ttcgccacag agatcgtcgc 59520 cgcctgccgc ggggcggtcg ccccggactt cccgatcacc ttccggttct cgcagtggaa 59580 gatgggtgct tatcaggcca ggctcgcggg atcgccgcag gagctcgaaa ccctgcttgc 59640 ccccctgtcg gacgccgggg tggacgcccc ccactgctcg acccgccgct tccacctgcc 59700 cgagttcgag ggatccgagc tcaacctcgc cggctggacg aagaaggtct cgggcaagcc 59760 cacgatcagc gtcggctcgg tcggcctcga caacgagttc atcgaggtgt tccaggggaa 59820 gccgggctcg gtcgccggga tcgacgcgct gctcgaccgg gtggagcgcg acgagttcga 59880 cctggtggcc gtcggccgcg cactgctcgg tgaccccgag tggatcacga agatcctggg 59940 tggccggacc gaggaactcc agcccttcca catggggctt ctgggtaccc ttcactgagc 60000 tcgttcacac ttcaagaatt ccaggcgacc gcatgcggca ctgattaccc gagtaatccc 60060 gtaatcaaca atcgatcacg gccactctgg ccgaatatgc acttctgata ttgcctcatg 60120 tttcccggat gcttcggctc cctcattctc gctggtgtgt caatagctat tatttgactt 60180 ttcatccagc ggtttgtagc cttctgggag cgcgactcga tgcccgctta tctggcataa 60240 catgggggag tatcggatga gcaatcagtc cacaaaattt ctcgagcccg ccattccgca 60300 acaactggcc aatcgccgcg cgccaacgcc gtcgcgccca gtgagccaac atgcccagca 60360 gcatccgccc atacaggccc agcaactacc gcccatccag gcccagcctc agctgcgcaa 60420 ggcgcgcggg gtgttgcgtg tcctcgttgt cgagagcggg gcgcccgccg cccagtccct 60 480 ggttctgagt ctggccaggc acgggtacca ggccgtgagc gtggacaccg gcagtaaggc 60540 gctccagaag taccgggaag cggatgtcct gctcatcgac ctggacctgc ccgacctgga 60600 cggcatcgag atctgccgtt tcgtcagggc cgcgtccggg gtcggagtca tcgttgtcac 60660 ggcccgcgac acggaactcg accgcgtgct cagcctcaag gcgggcgcgg acgactatgt 60720 ggtgaagccc tacagtctgc ccgagctgat ggcccgtatc gaagccgtca tgcgacgggt 60780 gcgcccgctg ccactgacca ccgaagtgat cagcagcggc ccgttgcgga tcgactcgcg 60840 cacccgtgag atccggctcg gcgacgagcc cgtcgaagtg acgcgcaagg agttcgacct 60900 gctttatctg ctggcctcgc ggccgggcag cgtcgtttcc cgaaagcaaa tcatgacact 60960 ggtctggggc gattcccgct cccagccggg tcgcacgatc gacacgcatg tcagtagtct 61020 ccgcagtaaa ctcggatcca gcagctggat catcacggcc cgcggtgtcg gtttccgatt 61080 cggtcatgcg taaattggtt ccgtgtattt ccgtgaactg gaaaatggtc actcgggaag 61140 aaagcgattt cccttcgcca ggcgcataac atgaactctc ggagaaaccc tcgccgacac 61200 gcaccggcgc aaacgacgag cccgcggccc catgggacgc gggctcgtcg catggggcag 61260 gaagcctgcc ggctgccgcg agtcggcctc ggcgggtgac ttcctgatcg gccggcaacg 61320 cgagccccca ttgcccgacg gctcgacgcc gacgcggccg tgccgccgcc tcgcaccgca 61380 tccggttcca cgcgcacgcc ggttccacaa cacccgtggc ggacacgcca cctctggtgg 61440 tcgggccccg tcctcggacg acactcggac agcacgccgg cccggaagcg cacaggctca 61500 cgcttcatcc gtcctccggc ggtggccgtc acgccaccat ccgaaccgcg tggcgccgtt 61560 gtgggccgag gacttcgacc gccgcgccgg accgcagggg ccttgctccg gcccgcccac 61620 gtggatgagc gagcacgctc gggttcgccc cccgggcggg gatcccctcg cggcccaccc 61680 gctcgcgcag gaggcccgct cgtgtcgtct ccgcggcccg ggaagctgcc catggtgccc 61740 atcccggcgg ccgtccacgc acaggcgtga gcgccggacc ccgctcaccc gggtgcaccg 61800 ggtgccgttg tccggcgccg cgccggcccg ctctgccgga ggggttatgc gttacgcccg 61860 cgccgtacag cctcgagccc cagcactccg gccgcgccca cgaccacgtg catcacggcg 61920 agtgaagctg ccgtgccgcc gtcggtctcg ctcgtgaggg ggccgatggc ggtgaggacg 61980 ccgagggctc cggacacgat ctggccgacc cgcagcagca ccggcttcca gcgggccgcg 62040 aacgcggcgg ccgtgacgcc gagcgccggc ggtacgaagg aggcgaagat gacgccgccg 62100 gcgacgatgt cgtcggcctt gccgttgagc tccagggtca gggaggcccc cgccgcgtcg 62160 gcgatgaaca ggacgatcag gttgacggcc gtcgcgacga gggcactgcc caccgcggcc 62220 tgccacaggg gcagtccgcg ccccgcgcga gccgtggggg aagagctgat ctcggcgggg 62280 ttgctgctgg tcatgaaagg gtcctccggt tgatgaatga gagcgtgggg ggtcaggacg 62340 ggttcaacga gaggccgtgc ctgaagacgt tgcgcgggtc gtacgtgttc ttgacccgct 62400 gcagccgggg gtggttgtcc ttgtagagga tcgtgtacca gggcacgccc gaggagttct 62460 ggtacgggtc cctgatgtcc acgtcggggt agttgacgta tccgccgtcc tgctggtcgt 62520 tcgggaccgg gaagccgccc gtggcgctgt ggacgccctt gcgcagcgtt ctgatccact 62580 cgacctcggc cgcgtcggcc gacggctcgg accagttggt cgagtaccag accttgaaga 62640 tggagtcccg ctgggccacg gccgtcgccg acgacgacac cgtgttgatc ttgcagccgt 62700 atgccgcgag gtacacgaga ccggcgccgg tggagtgctc gggatccgtc aacgaccggt 62760 acacgacgcc gacctgagct gccgtcagcg gcttgcgcag atgcccgccg atggacttgt 62820 gccgggagta cgtgccgaac gggggcgtcg gctcggtggc cgcctccacc cagggcatct 62880 cccgtgtgtc gaccgccggc gtgacaccga caccggccga caccgcggcc aggtagtcgt 62940 cgaacagctt cggggtggcg gcgagcgtcg cgtccatccg gatttccagc acgaacgacc 63000 cgaccagcga cgaacgcagt tggaaggagc tgtgcatggt ggcgtacggc gagtcgggcc 63060 cgctgttggc cgcgtgccag gcaccgtggt tgagcagcag ccgggtgaag ccggcctcgg 63120 tgatgtccga ccactgccag gtcacaaggg acttgcgcat ccgggcgggc ggcttgggca 63180 gcagcgcggt gggggctccc gacgcgcccg gcgtgcggaa ccagaacctg gtcacgatgc 63240 cgaactgacc gccgccacca ccggtgtgcg cccaccacag gtcgcggttc gggtcgctgc 63300 ggcggctggt cgcgacgatc gtacgagcct tgccgtcgct gcccgcgacc accacctcga 63360 ccgcgtacag atggtcgacc gacagtccgt agcgccggga cagaggcccg taaccgccgc 63420 cggacacatg cccgccgaca ccgacctcgg ggcagacgcc gccgggcagg gtcacgcccc 63480 agccctcgta cagcgccttg tagacctcgc cgagccgggc gccggactcg acggcgaagg 63540 cgttcatcga gctgtcgaag tagacccgct tcatctccga ggtgtcgacg aggagacgga 63600 actgggggtc gtcaaccagg ccgtcgaagc agtggccgcc gctgcgtacg gtgatccgct 63660 ggtccttgcg gatcgcctgg gtgaccgcct tctccacctg ggaggcgttg tgcaccagcc 63720 agatctcgtc cggggcggct cggaagcggg tgttgtagcc gcgccccgcc agctcggcgt 63780 agcgcgtgtc gccgggtctg acgatcacct cgggcggaac ggccgcggcg gcggtcgtgc 63840 cggcgtgggc ggccggtgcc acggcggaca ggcccgtcgt ggtaccggcc acaactatcg 63900 aggtgccaag gaacgtgcgg cgcgaaacca tgtcgagcac tcaccctttt ctcaagtacg 63960 tggaacggct ggagactgcc gccagccaag caggggctcc acgcggtccg ggcacagttg 64020 acacggatct gtcatagctc cgtacgaaac ctgatgctca gacccaaacc cacaaaagcc 64080 cgcccctccc gaagagccac ttggcgggct gtggtcgcgg caggccacga tgggcggggc 64140 cgggcggagg gcgtggcggc aggtcagggc ctctgtcgcg aacttcggac cggacgcgtg 64200 ctcacgacgg ctccggctgc caccccagcg cccgcgagat gccgcgcgcc gcgagccgta 64260 ccgcagggat caacgccggt gtctgcgcgt cgcgttcggg caccacgacg gagacggccg 64320 cggccaccga gccgtccgcc ccacggaccg gcgcggccac cgacagggcg tcgtcggtga 64380 tctgacgggt gctcaccgcc acgcccgcgt ggcggacttc ggcgagtacg cggcgcagga 64440 cggccccgtc cgtgatggtg tgcggggtga atccggcgag atgcccgccg cagtacgtct 64 500 cctggaaggc ggcctcgcag tgggcgagca gcgcgagacc cacgccggtc gcgtgcagtg 64560 gccagcgggc gcccacctgg atcttcacgc ccacggccga acggccggag atccactcta 64620 tgtagacgac ctcggagccg tcccggaccg ccaactgcac gttctcgtgg gtggcttcgt 64680 acaggtcctc cagatacggc agcgcgatct gccgcagcgc gagaccgcgc ggggagagcg 64740 ccgcgatctc ccacaggcgc aggcccacgt ggtagatccc ggacgcgtcg cgctccaggg 64800 cgccccagtc gctgagcgcg cccaccagcc ggtgggcggt ggtgagggag agcccggccc 64860 ggcggctgat gtccgtgagg gagagcgccg ggtggtcgtg gtcgaaggcg gcgagcacgg 64920 ccagcaggcg gtcgggggcg gacggcccgg tcatcggccg ggaccggtca tgagtcgagg 64980 ccagtcatgc gtcgaggccg gtcacgagtc gagttcggct tcggcgaccc gctgcagcag 65040 tgtgtgcagg gtgtcccgct cggcggggtc gaggggcagg agcaggtcgt tcgtcacccg 65100 caggcccgcc tcgtcggtgt cgcgcaggaa ggcgcggccg tcctcggtga gcaccacgat 65160 gcggctgcgg cggtcgtcgg gggaggggcg gcgctcggcg aagccgagct tctccaggtc 65220 gtcgaccaga ccgacgatcg cgctcgggtc gtagccgagc cgcgaactca gctctcgctg 65280 gagtgcgccc tccgaggtgg ccaggaaccg cagcagcgcg tagtgccgca gccgcagccc 65340 cgacgcctgc aggaacgtgt tgaacagctg ccccgaccgc agtcccagcc ggtacaggag 65400 atagccggtg tccgagtgca gcccgcgcat ccacggctcc tgcgagtcga tggcgtcggg 65460 gggcgtggcg tgctgctggc cggcgatggc gggctccctg gaccgggccc ctgcggggcg 65520 gtgcgtacgt gttccccaca gcatgccgca cagacgagtc ggcaacaact attgacgaca 65580 acaatgattg tctttagctt cgatctcgta atgctccgat ctcgtagccg tcctgccttc 65640 cgcaggtgat ccgtttctcg aagggaccgt cgtgcccagc atcgatctca ccggcaaggt 65700 cgccgtcgtc accggctccg gccgtggcct cggcctggcc tatgcgcacg ccctggccgc 65760 cgcaggcgcc tccgtggtcg tcaacgacgt ggacgaggac gtcgccgagc aggccgtcaa 65820 ggcgatcacc gaggcgggcg gcaccgccgt ggccgaggtg gtcccggtcg gcaccaccga 65880 ggccgcggac cggctggtgg gccgcgcggt gtcggagttc ggccgcctcg acatcctggt 65940 caccaacgcg ggcatccttc gggacaaggt cctgtggaag atgaccgacg aggacttcga 66000 cgcggtgatc accacccacc tcaagggcac cttcacctgc gcccgcgccg ccgccgtacg 66060 catgcgcgaa cagggcgagg gcggcacgct gatcctcgtc ggctccccgg ccggacagcg 66120 cggcaacttc ggccagacga actacgccgc cgccaaggcc ggcatcgccg ccatggcccg 66180 tacgtggtcg atggagctgg gccgtgcggg catcaccgtc aacgcgatcg tgccggtggc 66240 cgcaacggcg atgaccgaga ccatcccggc cttcgcgccc tacatcgagg ccatgcgcgg 66300 cggcgagccg ctgccggagt tcctccgcaa gggcgagggc ttcggcaccc ccgaagactg 66360 cgccgccctc gtcccgttcc tggcctccga ggccgcccgc ggggtgaccg gccaggccat 66420 cggcatcggc ggcgacaagg tcgccctgtg gtcccacccc caggagatca aggcggcgta 66480 cgctgacggt ggctggaccc ccgattccct cgccgacgtc tggccggcct cgctgggcgc 66540 cgagccgcag tcggtcggca tccccgcccc gaagttcccg gaggcgtgat ccatgacgaa 66600 catcgatgtc gaggaactcg tcgcgatcga cgtccacacc cacgccgagg tctcctccaa 66660 gggcacctcc tccctggacg acgacctgca cgaggcctcc agcgcctact tcaaggtcga 66720 gggcaaccgg aagccgaccc tggaggagac ggccgcctac taccgtgagc ggaagatggc 66780 tgccgtgatc ttcacggtcg acgccgagtc cgccaccggc accgcgcccg tcccgaacga 66840 ggaggtcgcc gaggctgccg ccgccaacgc ggacgtgctg atcccgttcg cctccatcga 66900 ccccttccgc ggcagggcgg gcgtcaagca ggcccgccgc ctggtcgagg agtacggggt 66960 gaagggcttc aagttccacc ccagcatcca gggcttcttc cccaacgaca ggtcggtcgc 67020 gtacgagctg tacgaggtga tcgaggagac cggcacgatc gccctcttcc acaccggaca 67080 gacgggcatc ggcgcgggcg tcccgggcgg cggcgggatc aggctcaagt actccaaccc 67140 gctgcacgtg gacgacgtgg ccgcggactt cccgcacctc aagattattc tggcgcaccc 67200 gtccttcccc tggcaggacg aggccctcgc ggtcgccacc cacaagccgg gcgtccacat 67260 cgacctgtcc ggctggtcgc cgaagtactt cccgccgcag ctcgtgcagt acgcgaacac 67320 gctgctcaag gacaaggtgc tgttcggttc cgactacccg gtcctcaccc cggatcggtg 67380 gatggccgac ttcgcgaagc tctccatcaa ggacgaggtc aggccgaaga tcctcaagga 67440 gaacgcggcc cggctgctcg ggctgacgaa gccgtagcgg acagccaagt gcaacgaagg 67500 acagaggcac cccatgtgcg atgaaggaca ggggcacgga catgcgcaat gagggactgg 67560 ggtcgtggcc cgcacgccgg gcccgcaaga ccccgcaccg caccgccctg atccacggcg 67620 acacgacggt cacctacgcc gggctgtacg agcgcaccac ccgcctcgca cacgccctgc 67680 gcgactccgg ggtgcgccgc ggcgaccgga tcgcctacct cggcccgaac cacccctcct 67740 acctggagac gctgttcgcc gcgggcaccc tcggggcggt cttcgtaccg ctcaacaccc 67800 gcctcgccgg acccgaactc gcctaccagc tcacggactc cggcgcgaag gccctcgtgt 67860 acgggcccac gttcggcggc ctcgtcgcgg ggctgcctgg caacagcgac gtgcgtacgt 67920 acgtcgaagt gggcgccgaa tacgaccagt tgctcgacat ggcctccgac gaggccatcg 67980 acgaacccgt catccccgac gacacctgca tcatcatgta cacctcgggc acgacgggcc 68040 ggcccaaggg cgcgatgctc acccacggca atctgacgtg gaacgcggtc aacgtcctgg 68100 tcgaccagga cgtgatcacc gacgaacgcg ccctggtctc cgccccgttg ttccacacgg 68160 ccgggctgaa catgctgacc ctgccggtcc tcctcaaggg cggtacgtgc gtcctggtcg 68220 aggccttcga tccggccgcc accttcgacc tgatcgagcg gcaccggatc accttcatgt 68280 tcggcgtgcc caccatgttc gagcacgtgg cccgccatcc gcgctgggcc gacgccgacc 68340 tgtcctccct gcggatgctc agctgcggcg gctcacccgt gccgaccccg ctcatcgccg 68400 cctaccagga gcggggcctc accttcctcc agggctacgg catgacggag gcctcgcccg 68460 gcgtgctctt cctggacgcc gagcacgcgg tcaccaaggc gggttcggcg ggcgtgccgc 68520 acttcttcag cgacgtccgc gtcgtacggc ccgacctcgc ccccgccgac atcggcgaga 68580 ccggggaagt ggtcgtacgc gggccgcatg tcatgcccgg ctactggggg ctgcccgacg 68640 agaccgctgc cgtgttccac gacggctggt tccgcagcgg ggacgccgcc cggatcgacg 68700 aggacggcta cgtcaccatc gtcgaccgcc tcaaggacat gatcatctcg ggcggcgaga 68760 acatctaccc cgccgagatc gaggaccagc tcctcgccca tcccgacatc gtcgagtgcg 68820 cggtcatcgg agtgcccgac gagaagtggg gcgaggtgcc gcgcgcggtc gtcgtgcccc 68880 gcgaggacgt cgcgctcgac cccgacgagg tcctcgcgtc cctcgccgga cgcctcgcca 68940 agtacaagat cccgaagtcg gtggtgctcg cggacgaact gccgcgcacg gcctccggca 69000 agctcctcaa gtcccgtgtc cgcaagcggt acggcaccaa ctcccagtcg aaggaagaca 69060 catgagcatc accgcgaacg gcatcgacga gctgaagaag ctcgccggca gtgacctcgg 69120 caccagcgag tggatcgagg tcacccagga gcgcatcaac acgttcgccg acgccacggg 69180 cgaccaccag tggatccacg tcgacgagga gaaggccaag gagggcccgt tcggcgcgcc 69240 catcgcgcac ggctatctca ccctctccct cttcatcccg ctcttcaccg agctgctgga 69300 cgtccagggc gtcaccacga aggtcaacta cggcctcaac aaggtgcgtt tcccctcacc 69360 ggtgaaggtc ggctcccgca tccgtctcgt cggaaagctg gcggacgtcg aggacgtggc 69420 ggggggtgtg cagatcacgg tcgacggcac gatcgagatc gagggcgcgc ccaagcccgc 69480 ggccgtgctc cagagcctgt cccgcttcta cgcgtgagcg ctccgctgtg agcgccattt 69540 cgtctgcggg ttcgtcgtgg ctggtcgcgc agttccccgc gcccctatgg ggcgcgaacc 69600 ggcaccaggt cgagaaggcg ttccgccgac tcccttgcga gtggggtcag tcgggcgtac 69660 accgtgcgga acgtcttctc ggcctgttcg gcgggccagt tgtccgggag gtgtttgacc 69720 ggtaggcgcg ggtcccggcg gacgacctgc agccactccg tcgacaggcg cagccgcagt 69780 gcgagagcct cgtcggcggc gggctccgtc gtctcccagc gctgccagcg gtgcgtgaac 69840 gcctcgtagc gcgtggccag ttcgcccagt gcccaggtct cctcgatcat ggcgccgatg 69900 tccatgcccg cgtccgcgtg ggcccggaag atcttcacat gggcggacag accgagttcg 69960 gcgacgatcg cggagacgtc ggcctcgccg ggtgcgatcc acagcccgcc gaacagcggg 70020 ccgaagccgc tccaggccaa ctgcgcgcgc agatcgtgcc gttgacgctg ccaggactcc 70080 ggcagcgaga agccgagcag cgtccaggag ccgtcccact gccggttgac ggccccgtcc 70140 tcccagatcc gctgctcgcc gtcccgcagc acggactccg agcgctcggt cagcccgaag 70 200 tacatccggc gtccctcgcg ctgccgcctg agcagatcgc ggcccaccat gcgcgtcagc 70260 gtcgagcggg tcgcgtgctc gccgacgccc gcgcgcccga agacgtcgat gacgctcccc 70320 gagtacacgc agacgtcacg gccgagcacc tggtcaccca ggaacgacag catgagggac 70380 tggggccgca gcgcctcgtc agtggtcacc ggcccacggt acgcccgtgg atcagctggt 70440 gaccaggtag tacgggcgct ccggccaccc cagtacacgc ggcccccgga ccggcctcac 70500 cggtggccgg cgacctccac gaacaccggg ttggagtaga accacgtgtc gagccacgga 70560 tcaccgtcgc ccggcgcgtg cgggatcggc ccgtgcgggt cgaccgacgc gcccaggtat 70620 ccggcgccgt gccggttgcc gtcgctgccg cgcagccgca cgtagaagga ctcgtccccg 70680 gcggtcagcg ggatgcgcag ggtgtacgtg cccttgcggc cggacacgtc cgccgtgtgc 70740 acgaccttgg tggccggtgc ccgccaggtg tcccggtcgg ccgccggacc gcgcaccgcg 70800 ccgcggatga cgtccacgtg ggccagcttg ggcaggatgt cgtgcgggtt gggccgcgag 70860 gccgtcgtca cggtcacgtt cagggtgagc ttctcaccct tgcggacccg cagccgactg 70920 cccagcgtga cgccccggcc gtggtcgcag tcccgcttca gccgtacgtc gagaccgtcg 70980 agcagatggc cgtggtcgag ccagacgcgg cccgcgcgca ggcccgccat caccgcgcgg 71040 tagccgtagc gggacacgcc gacgtgcgtg cggctgaact ggccgggcca gaagtcgccg 71100 ccgggctgtg gctcggtggt gttgaccggg tcgggcagcc ggcccgtgtt gtcgaagttc 71160 tgccccggca gccagtcgcc gttcttccag gtgtcgaaca ccacccggtg cacatcggag 71220 ttggtggtga tcgtgaacag cttgccctcg gagagcatgg cgtcccacat gccgccgacg 71280 gtcgccgtca tccagtcgaa gccgccgtac gtgaggtacg agtccgtcgg ataggcgggc 71340 caggagtcgg ccgacggctt gttctcgtac tcgccgcgta tcgacgtcgg accgcgccag 71400 ccagggatgg caccgccctg cgcacccggc gcgccctcca tgccgatcat gatctcgggg 71460 gccgcgtccc gccagttccg catctcgtgc ggggagtcga tgcccaggcg catcgggtgg 71520 ttggcgaaga ccagtacgtc gtccacgtag cccgaacggc gctgtgcggc cagccacttg 71580 atggccttga cggcgtgcgc ctcgttgcgc ggggtgtcgg ggtgggtggg cgcgcccgcc 71640 gtgtagccga gcagcttgcc gtcgtaggcc agctcgaact gcgtgagcag gtccacctcg 71700 tggcggccgg gcgccgcgaa gatcgtgcag tgctcggcgc ccgggatgta ccactccagg 71760 ccctggaaga tcagctgacg cgggttcttg gcgcgtgcct tgaggatctc ctcgtgctcc 71820 agcttcgccc cgtactgggc gtgcccgaag ttggagtgct cgttgaacac catccagtcg 71880 aggccgtact tggcgctcgc ctgcgccagt tgggagaacg tgtacttcgc gtcgtggctg 71940 tagacggagt gcacgtggtg gtcgccgacg aggtaggcga ggcgcgggtc gttgccgccg 72000 aaacccttcg cgctcgccat ggcgggcgtc gccagcgatc cggcggcgaa ggcggcgccg 72060 aacaggcccg cgcggcgcat gagctgacgt cgggacacgc cctgcgcgtc gagggcggcg 72120 ggggagacgg acggatctgc ccaggcgggc agctgctgct cggtcatggt ctggggagtc 72180 cctcggagtt cgatggatca gtggttcatg aacgacgtga cgggcagggc gcgttcgacg 72240 atccgcacgt caccgagccg tccgtgcagg atctggtcga tcttcccggc gtactcgtag 72300 ccgccgagga tccagggcag gccgacggag gtgatgccga cggacgacgc cttcgggttg 72360 cgcaccaccg ggcagccctg cacatacagg gtggtgtgcc ttccgtcgtt gacgacggcg 72420 atgtgccacc acgtctccaa cggtgtctcc tggccccagt tggtcgcgat tccctgctgg 72 480 ttgagggggc gcatggccca ctggggctcg cggtcgttcg acagcgacag cgtggcgatg 72540 ggttcgtccg ggtcgtcggc ggtcttcccc gcggcaccgc ccgtaccggt gcggccgagc 72600 agcccggccc acgcgtcgtg cgaggggtcc cagtcggcgg gcagccggta gaaggcctcg 72660 atggtgtaac ccgccttgaa agtggccgag ttgaggggcg cgccgtcgac cgtccgcagg 72720 tacgcgccct tcagcggggg cttgccgccg tggaagtcca ggctgccgtg gcccggttgg 72780 tcggggtggt ggtccgacga ccaggtgagc gtgccgcccg cgaccgtgac cagggagagg 72840 tcgttgccgc gcccggacac atcgcggacc gtgccggtca cggcggcccc gtcggacttc 72900 ccgtcgaagc gccagtacgc gaccgtgccc ggtatcagca tcttcgacgc gggacgcgcc 72960 gggcgcgcgg gcaccggggc gaagccggcg aagcgctcct cgaagtcgat cccgaccgag 73020 aaccggtcgg cgtcaccact gagttcgctc tcctggcgct ccagctcgtt gagccccttc 73080 gcggcccggc cgaggatcca cggcgacacc gtctccacgt ccacggtgtt ccggtcgagg 73140 tcgaagcggt agagccggat catcgccgca ccgccgaagt accggttctg atagttcgtc 73200 agatgcaggt ggacgtcatg tcccgccgcg ttcttgcgag ttgcccgcgc cgcgggccag 73260 tagtggccgt tgagggtgag gaagatctgg tcgtggtcct cgatcagctg gtcccacaac 73320 tgctgtccgt acgaggagag cgagtcgtcc tcgtcgacca gctcgtgcgt cgtgaggatc 73380 accggagtct tcgggtgctg ggcgatgacg tccttggccc acgcgaagcc cttcgccgac 73440 agccgccagt ccagcgcgag caccatccac tcccgcccgg ccgcccggaa gaggtggaag 73500 gtgttgtacc cgtctgggga ggcacccccg tacgtggacc tgcccttgaa gcggtccggg 73560 ccgaagacgt ccaggtacgg gctcgggccg cgctggtcgt ccgtcgacga cttcacgtcg 73620 tggttgcccg ccaggacgct gtagccgacg ccctgccggt cgagcagccg gaacgcctgg 73680 ccgatcgccg cgcactccgc cttcgcgccg ttctgcgtca ggtcgccgag gtgggacagg 73740 aacacgatgt tctcgtcgcg cccgtgctcc agcagatagc gcagggacgc ctcgacgggc 73800 gccttgtcga tgctgggccc gtcgaacagg tactgcgtgt cgggcatcac ggcgagcgtg 73860 aagcggcggc tgtcggggtc gggtctccaa cgggccttcg agcccggcac cgcctgcgtg 73920 gtggccgccg gcagcgcgac cgctgccgtg gccaccccga tcagcgcggt cgcccgcagg 73980 acgttccgtc gtccggcgcc ggcctgctcg gcctcgcaga gggtctggtc gtgctcatgc 74040 gaagtacaca cgtctgctcc gtgagaaggg ggacagctcg ggagaggggg gtggcgattc 74100 gagagggggt ggcgactcgg gagggggagg aggcgaccag gagcgggacg gcggccggga 74160 tcagagcacg cgcagcgtcc acgcccagcg gtgcgtgccc ggcgtgacga ggtactcggc 74220 ggtggtgtcg ggcccgcacg acgccgtgcc gagcccccgg tgcgcggcgt cgaggtgcac 74280 gacacagccg ggccgcggca ccagctcgtc gtggtgcgcg gcagccgcca ggtccccggc 74340 ccggtaccgg ttgacggaga cctgccccgg cgccccgagc tccacggtca gccccgtggc 74400 gtccggcgcc gacagcgtga accgccgcac accgtgccgg ccgccgctct cctgaggccg 74460 cagataaggg gtgaacagct cgtccaccgg aagcgagtgg tgcccgacgg gcgcgcccgt 74520 gctccggtcc gggtaggact cccaggggcc ctgcccgtac cagtccagga cgtcgagccc 74580 ggcgaccgtc tcgaagaccg tgccgacgcg ggccacgtcg tcgaggccct ccggcagttc 74640 ggccgactcc tcgacccgga ggccgccggc gaccggggtg aacacctgct cgtggcggac 74700 gggcccggcc ccggtcgcgt actcggcgac cacgacgacc cgagggccct cctcacggac 74760 gtccacgacc ttgcgtacga gctcgtcgag gccccagtcg tgccagcggg ccgccatgcc 74820 gcccagttcg tcgttgtcgg tgggcgcccg ccacagcgag aggacgggcg cggaggtcag 74880 cagcgggtgg accaggagtg cgtccgcgtc cagcgtgacg gccgggccgg acgcgggctc 74940 cggcgtcgcc tcggcgacgg ccgcgcccag ccggacctgc ggcagacaca cctcggtgcc 75000 gaggggagcc caggcctcgt cgtccgcggt gacgacccgc agcgtcagcc aggactcgcc 75060 ctcctgcacg gggaacggca gtggcaccgc cgcggactcg cccggcagta cgtcgggcag 75120 ctcggccggt gccgtcaccg tggtgccgtc cgcgaggacc aggcaccact cggcggccag 75180 ccattccagg tcccggaagt gctgccggtt gtggaccagg agcacgccct ggtcataggt 75240 gaggcggacc ggtgccgcga tctcccggtg ctcgtacatc acgggcttgg gcgtgcggtc 75300 cgggaacacc acgccgtcgg cgatgaacgc cccgtcgtgg atggtctcgc cgaagtcgcc 75360 gccgtaggcc cagcgatggc cgggcgcggc gactccgtgc tcgtacagcc cggcgccccc 75420 acgcccggca ggtcttccgt cgttcacacg ctggagaatg ccgtggtccc agaactccca 75480 gatgaagccg ccctgaagac ctggggtgga ctcgatggcc gcccaggtgt cggcgagcgt 75540 tccgttgctg ttgcccatgg cgtgcgagta ctcgcactgg atgagcggca tggtctgctt 75600 ccccgaccgg gcgtgcgcga cacagtcctc gatcggcgcg tacatcgggc agacgatgtc 75660 ggacgcggtc gtcggatccg cccagtcgag cttggccgcg ccctcgtact ggatgggtcg 75720 ggtcgggtcg tggcgccgga cccagcccgc cgccgcgtcg tggttggctc cgtagtcgga 75780 ctcgttgccg agcgaccaga tgatgaccga cgggtggttc ttgtcgcgca gcaccatgcg 75840 cgagacccgg tccacgaagg cgggcagata gcgggggtcg tcggcgatct cgtgggcgtg 75900 gtcgtgcgac tcgatgttcg cctcgtccac gacatagaag cccagctcgt cggcgaggtc 75960 gtacagcgtc gggtcgttcg ggtagtgggc ggtgcggatc gcgttgaagc cgaagcgctt 76020 catgacgacc aggtccgcgc gcatgtcgtc cgcggtgacc gtgcggccgg tcagcgggtg 76080 gaagtcgtgc cggttgacgc cccggatgta gacgcgttcg ccgttgacca gcaggtcccg 76140 gccgcggatc tcgacgtccc ggaacccgac ccggtggtgg gaggtgtcgg cgaccgtacc 76200 gtcggcccgg tgcaggcgca cggtcaggtc gtacagctcg ggtgtctcgg cggtccacgg 76260 ccatacgttc tcgacggtgc cccgcagccg ggcctcaccg aggaagtcgg acacccgcat 76320 gtcctcggcg ttgaagcggt cgaactcggt gtcctgggct aggagttggc cctccagcgc 76380 cccggtgacg taccaccccc gcggcagcgg tcccgtcgcg tcgcgcacct ggcagtcgac 76440 ccggagccgc ccgtcccgcc gcgcccgcac ggtcacgtcc gcgagataca gcgggtcggt 76500 cgcgtacagc agcaccgagc gggtgatacc ggcgtgccac cactggtcct ggtcctcgat 76560 gtgcgaggcg tcggaccact tgacgacggt gaaccgtacg gtcgccgggt caccggggcg 76620 tacgacgtcg gagaggtcga actcggccgc gaggtgggag tccttggaga tgccgaccgg 76680 tttgccgttc acatggacca ggagcacgct ctcggccgcg ccgacctgga gcacgatccg 76740 gcgtccggcc cactcggcgg ggatgtcgac ggaacgctcg tatatgcccg tcgggttggc 76800 ggcgggcgag gcgggcggga attcgcccca cggcatctgc gcgttggtgt actgcggcag 76860 gtcgtcgccg ctctgggtgg tccaggtgcc cggcacctcg gtccgggtcc actcgcccga 76920 gcagcccatg ggcgagggca gcagctggaa gtgccaggag ccgtccaggg acagcgcacc 76980 ggagcgccgg tccaccgcgt tcatggccag ccgcccccag gaggtcacct ccggtgcctc 77040 ccaggggcgc agggcgatca ggtgatcgcg tcgcatcagc gaatggctcc cttgccgaga 77100 tccgcgatga tctgcttggc gccgatcgcg aagacgatca gcagcgggat gagggccagt 77160 accgcacccg tcatgaccat cccgtagtcc gtggtgccgt ggacgccgtt gagctgggac 77220 agcgcgacct ggagggtcac gttgtccggg ttggtgaggg cgatcaacgg ccaggcgtag 77280 tcgttccact ggcccatgaa ggtgaagatg ccgaggaagg cgagaccggg ccgcacgacc 77340 ggcagcgcca cgtgccagta ctggcgcatg aagctggccc cgtcgagctt ggaggcgtcg 77400 agcagctcgt cgtggatcgc gctcttcatg tactgccgca tccagaagat gccgaacgcg 77460 ttcgcggcgg ccggcactat cagcgaggtc atcgagccga tccagccgag cttcgccatg 77520 atccgatatc tctagagccg ccccgcaggg 77550 <210> 2 <211> 465 <212> PRT <213> Streptomyces sp. SCC-2136 <400> 2 Met Leu Ser His Thr Leu Arg Ser Arg Gly Glu Ala Ala Leu Ala Asp 1 5 10 15 Arg Leu Ala Leu Ser Ala Ala Thr Thr Glu Gly Ser Gln Leu Ala Thr 20 25 30 Ala Asp Phe His Gly Trp Leu Ala Glu Arg Gly Arg Ala Asn Ala Phe 35 40 45 Arg Val Asp Arg Ile Pro Phe Ala Glu Leu Gln Gly Trp Ser Phe Glu 50 55 60 Glu Ser Thr Gly Asn Leu Val His Arg Ser Gly Arg Phe Phe Thr Val 65 70 75 80 Glu Gly Leu His Val Thr Glu Arg Asp Gly Pro Tyr Gly Asp Gly Pro 85 90 95 Tyr Ala Asp Trp Tyr Gln Pro Ile Ile Lys Gln Pro Glu Val Gly Ile 100 105 110 Leu Gly Ile Leu Val Lys Glu Phe Asp Gly Val Pro His Phe Leu Met 115 120 125 Gln Ala Lys Met Glu Pro Gly Asn Pro Asn Leu Leu Gln Leu Ser Pro 130 135 140 Thr Val Gln Ala Thr Arg Ser Asn Tyr Thr Lys Ala His Gln Gly Ala 145 150 155 160 Asp Val Lys Tyr Ile Glu His Phe Val Gly Pro Gly Arg Gly Arg Val 165 170 175 Val Ala Asp Ala Leu Gln Ser Glu His Gly Ser Trp Phe Phe Arg Lys 180 185 190 Ser Asn Arg Asn Met Ile Val Glu Ala Val Gly Asp Val Pro Leu Leu 195 200 205 Asp Asp Phe Cys Trp Leu Thr Leu Gly Gln Ile Gly Glu Leu Leu His 210 215 220 Gln Asp Asn Val Val Asn Met Asp Ser Arg Thr Val Leu Ser Cys Leu 225 230 235 240 Pro Leu Pro Ala Thr Ala Ser Gly Ala Leu Leu Thr Asp Ile Glu Leu 245 250 255 Leu Ser Trp Ile Thr Gly Glu Arg Ala Arg His Asp Val His Ala Glu 260 265 270 Arg Val Pro Leu Ala Gly Leu Pro Gly Trp Arg Gln Asp Glu Met Thr 275 280 285 Val Glu His Glu Glu Gly Arg Tyr Phe Asn Val Val Gly Val Ala Val 290 295 300 Gln Ala Gly Asn Arg Glu Val Thr Ser Trp Thr Gln Pro Leu Phe Glu 305 310 315 320 Pro Val Gly Thr Gly Val Thr Ala Phe Leu Thr Gln Glu Phe Asp Gly 325 330 335 Val Pro His Val Leu Val His Ala Arg Val Glu Gly Gly Phe Leu Asp 340 345 350 Thr Val Glu Leu Gly Pro Thr Val Gln Tyr Thr Pro Gly Asn Tyr Ala 355 360 365 His Leu Ala Glu Lys Asp Arg Pro Pro Phe Leu Asp Ser Val Leu Asp 370 375 380 Ala Ser Pro Asp Arg Val Arg Tyr Gly Ala Val His Ser Glu Glu Gly 385 390 395 400 Gly Arg Phe Arg Asn Ala Glu Ser Arg Tyr Leu Ile Ile Asp Ala Ala 405 410 415 Glu His Asp Ala Pro Leu Asp Pro Pro Gly Tyr Thr Trp Val Thr 420 425 430 Pro Gly Gln Leu Thr Trp Leu Val Arg His Gly His Tyr Leu Asn Val 435 440 445 Gln Val Arg Thr Leu Leu Ala Cys Leu Asn Ala Leu Pro Ala Arg Ala 450 455 460 Arg 465 <210> 3 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Primer <400> 3 cacttcgggg gcgagtcgca cgt 23 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Primer <400> 4 gggccgtagt tgttcgagca 20 <210> 5 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> Primer <400> 5 ggatcccata tgctgtccca caccctt 27 <210> 6 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Primer <400> 6 gaattcccgc gctcaacgtg ccct 24

Claims

Having the nucleotide sequence of SEQ ID NO: 1 Sch47554 and / or Sch47555 biosynthetic gene clusters.

The method of claim 1, wherein the gene cluster is Streptomyces sp. SCC-2136 (ATCC55186) Sch47554 and / or Sch47555 biosynthetic gene cluster, characterized in that it is derived from.

DNTP-4-keto-6-deoxyglucose-2,3-dihydratase having the amino acid sequence of SEQ ID NO: 2.

The gene encoding the dNTP-4-keto-6-deoxyglucose-2,3- dihydratase of claim 3 ( schS2 ).

The gene ( schS2 ) according to claim 4, having a nucleotide sequence of the region 33361 to 34758 of SEQ ID NO: 1 or a nucleotide sequence complementary thereto.

Recombinant vector containing the gene of claim 4.

Bacteria transformed with the recombinant vector of claim 6.

A method for producing dNTP-4-keto-6-deoxyglucose-2,3-dihydratase, comprising culturing the transformed bacterium of claim 7.