CN101691575B - Biosynthetic gene cluster of sanglifehrin - Google Patents

Abstract

The invention relates to a biosynthetic gene cluster of sanglifehrin, which relates to related information of clone, whole sequencing, sequence analysis, gene function research, in vitro biochemical research and application thereof of a natural product sanglifehrin which is produced by streptomyces flavolus and has immunosuppressive activity. The whole gene cluster can contain 24 genes, namely 1 NRPS gene, 7 type-I linear PKS genes, 1 type-I repeated PKS gene, 8 precursor synthetic genes, 2 regulatory genes and 5 functional unknown genes. The generation of the sanglifehrin can be blocked by operating the genetics of the core functional genes. Genes and protein provided by the invention can be used for research and development of new medicine or research and development of other products with industrial and agricultural application prospects.

Description

A kind of biological synthesis gene cluster of SFA

The present invention is that priority date is that on 09 24th, 2008, priority number are 200810200388.4, denomination of invention is the patent of invention of the continuation application of " biological synthesis gene cluster of SFA ".

Technical field

The invention belongs to microbial gene resource and genetically engineered field, be specifically related to the clone of the biological synthesis gene cluster of immunosuppressor SFA (Sanglifehrin), sequential analysis, functional verification in body, external biochemical test and application thereof.

Technical background

The immunosuppressor SFA is by the polyketone of faint yellow streptomycete Streptomyces flavolus DSM 9954 generations-poly-peptide class heterozygosis natural product [J Antibiotics 1,999 52 466-473, ibid 1999 52474-479].In being separated at present more than 20 kind Sanflifehrin analogs, with the activity the highest [J Biol Chem 2,005 280 21965-21971] of SFA A (SFA).SFA, 22 membered macrolide skeletons are comprised of a polyketone carbochain and a tripeptides peptide chain in view of structure, the latter comprises two alpha-non-natural amino acids: (S)-m-tyrosine be connected S)-pyridazine acid and connecting with the amido bond that forms at pyridazine acid 1-position N, be a unique example in numerous natural products that contain pyridazine acid structure of finding so far.Another one volution unit is connected with macrolide by a polyketone long-chain, forms the basket type structure.Spirane structure has been concentrated altogether nine chiral centres (full molecule is 17), and a quaternary carbon center is contained in the centre, and this unique chemical structure is not yet found in other natural product at present.

SFA has very strong immunosuppressive activity, can suppress simultaneously HIV infects [J Biol Chem 2003 27843202-13, J Virol 2,004 78 12800-8] and stops the serious cardiac muscle dead [J Biol Chem 2,002 277 34793-9] that causes due to the perforate of plastosome MPTP pathologic.Immunosuppressor S-Neoral (Cyclosporin A with present clinical use, CsA), FK506 compares with rapamycin (Rapamycin), there is more similar mechanism of action still to have again different effect target spot [J Immunol 2,001 166 5611-8, ibid2001 166 7165-71].CsA acts on cyclophilin (CypA) [Science 1,984 226 544], and FK506 and rapamycin act on FKBP and form mixture [Science 1,991 251 283-287]; And CsA-CypA and FK506-FKBP mixture act on identical target protein calcineurin (calcineurin), thereby suppress calcineurin to phosphatase activity blocking-up cytokine (cytokines) generation of Serine and Threonine, especially having blocked transcribing of interleukin II (interlucin, IL-2) finally causes the T cell to be blocked at G ₀-G ₁Phase [Cell 1,991 66 807-815].The Rap-FKBP mixture acts on protein kinase FRAP (also referred to as RAFT or mTOR) [Nature 1,994 369 756-758], has stoped the phosphorylation of IL-2 acceptor on the T cell, makes the T Growth of Cells be blocked the phase at G1-S.And the effect protein of SFA-CypA is unknown so far.

Due to the effect protein of failing to find SFA-CypA always, so also someone thinks that the SFA immunosuppressive activity is not by the effect of SFA-CypA complex body form.Science group studies numerous over nearly 3 years are found, thereby SFA can competitively stop the transcription site of NF-KB and P53 upstream region of gene in conjunction with activation P53, and then the phosphorylation of the Cyclin E-cdk2 of Inhibitory signal passage downstream causes the high phosphorylation of Rb that suppresses feedback IL-2, make cell to IL-2 not " sensitivity ", thereby can make cell rest on the G1-S phase [J Biol Chem 2001 27643534-40].Secondly SFA has suppressed IL-12p with a kind of mechanism of the unknown not affecting in human dendritic Growth of Cells situation ⁷⁰Generation [J Immunol 2,003 171 542-6], IL-12p ⁷⁰Playing a part key when regulation and control Th1 and NK cell proliferation, is the bridge that connects innate immunity and acquired immunity.Immunosuppressive drug due to existing listing can produce some serious renal toxicitys and maincenter toxic side effects [J Am Chem Soc2002 124 4257-70] in addition, therefore hindered the application (for example calcineurin is the effect source of immunosuppressive activity and the toxicity of CsA and FK506 simultaneously) aspect some immune dysfunction diseases, Novartis Co.,Ltd ought develop SFA for the immunosuppressor of high-efficiency low-toxicity of new generation for this reason.

Utilize the X diffraction to show to the structure activity study of SFA macrolide fragment and CypA effect that the tripeptides structure is embedded in the ditch of CypA and most important to binding constant, and the carbonyl on the hydroxyl of 17 and 14 side chains is for not contribution of keying action; Saturated C18-C20 position trans double bond disappearance makes binding constant reduce by 7 times, infer that trans double bond has stabilization [J Am Chem Soc 2,003 125 3849-3859] to conformation, the simultaneous computer modeling effort finds that also there is certain effect [FEBSLetter 2,003 555 335-340] SFA volution unit to stablizing the SFA-CypA avidity.The crystalline structure of complete SFA-CypA mixture shows that the calmodulin binding domain CaM of SFA-CypA and CsA-CypA is basic identical, and main and W121 all, R55, and H126, the residues such as N102 and Q63 interact; C24-C32 chain between volution and macrocyclic structure and the I57 of CypA, T119 and W121 residue have model ylid bloom action, and the existence of comparing in addition with the crystalline structure polyketone long-chain of CypA has changed the W121 orientation; The volution unit only has the methyl of C45 and the I57 of CypA and the side chain of F60 that model ylid bloom action [JBiol Chem 2,005 280 21965-21971] is arranged.

Simultaneously, SFA-CypA can with the dimeric forms stable existence, confirm also that by gel filtration chromatography dimer is the stable existence form in solution.According to crystal analysis, find all deep imbedding in dimer of parts all except volution and α ketone group butyryl side chain.Although the trans double bond of C18-C22 is not directly and CypA residue effect but have model ylid bloom action to be conducive to the combination of dimer mixture with the m-tyrosine of another molecule SFA; There is van der Waals interaction at the C26-C28 place of two molecule SFA; The R148 of the W121 of a part CypA and another molecule CypA has direct hydrogen bond to form in addition.

The result of structure activity relationship provides solid basis for the research and development immunosuppression, people were the binding constant of concern and SFA and CypA in the past, dimeric discovery makes everybody begin to rethink research strategy, not only to avoid binding constant to reduce to the modification of SFA structure, more will avoid affecting dimeric formation.New guiding theory has been arranged, and the effect mechanism of illustrating SFA will become and more be hopeful.

The inventor is take microbe-derived SFA as target molecule, from clone's biological synthesis gene cluster, the method that adopts microbiology, molecular biology, biological chemistry and organic chemistry to combine is studied its biosynthesizing, disclose the enzymatic mechanism of the unique chemical structure formation that comprises pyridazine acid by the research to its biosynthesizing mechanism, use on this basis the principle of metabolic engineering, the biosynthetic pathway of rational modification SFA, explore Stability Analysis of Structures, active better, and can pass through the mass-produced medicine of microbial fermentation.

Summary of the invention

The present invention relates to biological synthesis gene cluster clone, sequential analysis, functional verification, external biochemical test and application thereof that a kind of polyketone-non-ribosomal with immunosuppressive activity that is produced by faint yellow streptomycete (Streptomyces flaveolus) gathers peptide natural product SFA.

In the present invention, whole gene cluster can be nucleotide sequence or the complementary sequence (sequence 1) that comprises 20 genes, wherein 1 non-ribosomal gathers peptide synthetase (NPPS) gene sfaD, comprise altogether 3 modules, 10 functional domains are responsible for the formation of the poly-large ring skeleton of peptide moiety; 5 linear polyketide synthases (PKS) gene sfaE, sfaF, sfaG, sfaH, sfaI are responsible for the formation of volution, polyketone long-chain and the large ring of polyketone part skeleton; 1 repetition type polyketide synthases gene sfaK comprises 4 functional domains, is responsible for the biosynthesizing of the six special extension apparatus of carbon; 10 gene sfaA, sfaB, sfaJ, sfaM, sfaN, sfaP, sfaQ, sfaL, sfaO are responsible for the biosynthesizing of start element and the front building block of alpha-non-natural amino acid; The gene sfaS of 1 regulatory gene sfaC and 1 coding MbtH albumen of with NRPS regulation and control being associated relevant to the SFA fermentation yield, and 1 unknown function gene sfaV1.

The present invention also provides the nucleotide sequence of position hydroxylase between a coding phenylalanine, and the aminoacid sequence of its coding is arranged in sequence 4, and called after sfaA, its nucleotide sequence are arranged in sequence 1 17252-17854 base place.

The present invention also provides a coding ornithine 5-N oxidasic nucleotide sequence, and the aminoacid sequence of its coding is arranged in sequence 5, and called after sfaB, its nucleotide sequence are arranged in sequence 1 18088-19088 base place.

The present invention also provides the nucleotide sequence of an encoding transcription regulatory factor, and the aminoacid sequence of its coding is arranged in sequence 6, and called after sfaF, its nucleotide sequence are arranged in sequence 1 19193-19888 base place.

The present invention also provides a coding to comprise the poly-biosynthetic nucleotide sequence of the large ring skeleton of peptide moiety of being responsible for that C A PCP C A PCP C A PCP C non-ribosomal gathers the peptide synthetase functional domain, the aminoacid sequence of its coding is arranged in sequence 7, called after sfaD, its nucleotide sequence are arranged in sequence 1 19885-30714 base place.

The synthetic nucleotide sequence of responsible volution part biological that the present invention also provides a coding to comprise ACP KS AT DH ER KR ACP polyketide synthases functional domain, the aminoacid sequence of its coding is arranged in sequence 8, called after sfaE, its nucleotide sequence are arranged in sequence 1 30703-37360 base place.

The present invention also provides a coding to comprise the biosynthetic nucleotide sequence of responsible polyketone long-chain of KS AT ACP KS AT KR ACP KS AT KR ACP polyketide synthases functional domain, the aminoacid sequence of its coding is arranged in sequence 9, called after sfaF, its nucleotide sequence are arranged in sequence 1 37394-50014 base place.

The present invention also provides a coding to comprise the biosynthetic nucleotide sequence of responsible polyketone long-chain of KS AT KR ACP KS AT DH ER KR ACP polyketide synthases functional domain, the aminoacid sequence of its coding is arranged in sequence 10, called after sfaG, its nucleotide sequence are arranged in sequence 1 50017-60903 base place.

The biosynthetic nucleotide sequence of responsible polyketone part large ring skeleton that the present invention also provides a coding to comprise KS AT DH KR ACP KS AT DH KR ACP KSAT KR ACP KS AT KR ACP KS AT DH KR ACP KS AT DH KR ACP polyketide synthases functional domain, the aminoacid sequence of its coding is arranged in sequence 11, called after sfaH, its nucleotide sequence are arranged in sequence 1 60908-85820 base place.

The biosynthetic nucleotide sequence of responsible polyketone part large ring skeleton that the present invention also provides a coding to comprise KS AT DR ACP DS AT DH KR ACP polyketide synthases functional domain, the aminoacid sequence of its coding is arranged in sequence 12, called after sfaI, its nucleotide sequence are arranged in sequence 1 85823-96037 base place.

The present invention also provides a coding zinc to refer to the nucleotide sequence of desaturase, and the aminoacid sequence of its coding is arranged in sequence 13, and called after sfaJ, its nucleotide sequence are arranged in sequence 1 96225-97388 base place.

The present invention also provides a coding to comprise the nucleotide sequence of the repetition type polyketide synthases of KS AT ACP OX functional domain, the aminoacid sequence of its coding is arranged in sequence 14, called after sfaK, its nucleotide sequence are arranged in sequence 1 97396-101940 base place.

The present invention also provides the nucleotide sequence of a coding acyltransferase, and the aminoacid sequence of its coding is arranged in sequence 15, and called after sfaL, its nucleotide sequence are arranged in sequence 1 101936-103210 base place.

The present invention also provides the nucleotide sequence of a coding short chain dehydration/reductase enzyme, and the aminoacid sequence of its coding is arranged in sequence 16, and called after sfaM, its nucleotide sequence are arranged in sequence 1 103210-103926 base place.

The present invention also provides the nucleotide sequence of a coding Acetolon acid enzyme, and the aminoacid sequence of its coding is arranged in sequence 17, and called after sfaN, its nucleotide sequence are arranged in sequence 1 104001-105023 base place.

The present invention also provides the nucleotide sequence of a free acyl carrier protein of coding, and the aminoacid sequence of its coding is arranged in sequence 18, and called after sfaO, its nucleotide sequence are arranged in sequence 1 104941-105342 base place.

The present invention also provides the nucleotide sequence of a coding class asparagine synthetase, and the aminoacid sequence of its coding is arranged in sequence 19, and called after sfaP, its nucleotide sequence are arranged in sequence 1 105366-107216 base place.

The present invention also provides the nucleotide sequence of a coding free sulphur esterase, and the aminoacid sequence of its coding is arranged in sequence 20, and called after sfaQ, its nucleotide sequence are arranged in sequence 1 107366-108145 base place.

The present invention also provides the nucleotide sequence of a coding crotonyl-CoA reductase enzyme, and the aminoacid sequence of its coding is arranged in sequence 21, and called after sfaR, its nucleotide sequence are arranged in sequence 1 108150-109511 base place.

The present invention also provides the nucleotide sequence of a coding MbtH family protein, and the aminoacid sequence of its coding is arranged in sequence 22, and called after sfaS, its nucleotide sequence are arranged in sequence 1 109583-109795 base place.

The present invention also provides the nucleotide sequence of a coding agnoprotein, and the aminoacid sequence of its coding is arranged in sequence 23, and called after sfaV1, its nucleotide sequence are arranged in sequence 1 109776-110309 base place.

The present invention also provides the gene of 4 coding agnoproteins, called after: sfaU1, sfaU2, sfaV2, sfaV3 except above-mentioned 20 genes.Wherein sfaU1 is arranged in sequence 2, and its nucleotide sequence is arranged in sequence 1 14976-15413 base place.SfaU2 is arranged in sequence 3, and its nucleotide sequence is arranged in sequence 1 15596-16063 base place.SfaV2 is arranged in sequence 24, and its nucleotide sequence is arranged in sequence 1 110257-110907 base place.SfaV3 is arranged in sequence 25, and called after, its nucleotide sequence are arranged in sequence 1 111035-111451 base place.

The biological synthesis gene cluster Protein sequence of SFA can be also that the protein sequence with aforesaid 20 genes or 24 coded by said gene has at least 80% homology degree.

The complementary sequence of sequence 1 can obtain according to DNA base complementrity principle.The nucleotide sequence of sequence 1 or partial nucleotide sequence can be by polymerase chain reaction (PCR) or with the corresponding DNA of suitable digestion with restriction enzyme or use other suitable technology to obtain.The present invention also provides the approach that obtains to comprise at least the recombinant dna plasmid of DNA sequence dna in partial sequence 1.

SFA of the present invention can be all SFA analogs that produced by similar biosynthetic pathway, SFA A for example, B, C, D, E etc.Each albumen of SFA biological synthesis gene cluster coding of the present invention is used for catalyzing and synthesizing the corresponding all analogues of immunosuppressor SFA and family thereof.

The present invention also provides the approach of the interrupted microbe of SFA biosynthesis gene, and the gene of one of them includes the nucleotide sequence in sequence 1 at least.

Nucleotide sequence provided by the present invention or partial nucleotide sequence, the DNA that can utilize the method for polymerase chain reaction (PCR) or comprise sequence of the present invention obtains the gene similar to the SFA biosynthesis gene as probe with methods such as Southern hybridization from the other biological body.

The cloned DNA that comprises nucleotide sequence provided by the present invention or at least part of nucleotide sequence can be used for more library, location plasmid from faint yellow streptomycete Streptomyces flaveolus genomic library.These library plasmids comprise the partial sequence in the present invention at least, also include the DNA that in Streptomyces flaveolus genome, former adjacent domain is not cloned.

Comprising nucleotide sequence provided by the present invention or at least part of nucleotide sequence can be modified or be suddenlyd change.These approach comprise insertion, displacement or disappearance, the polymerase chain reaction, mistake mediation polymerase chain reaction, mutation site-specific, homotactic reconnecting not, the different piece of sequence or carry out orthogenesis (DNA shuffling) with the homologous sequence in other sources, or by ultraviolet ray or chemical reagent mutagenesis etc.

The clone gene that comprises nucleotide sequence provided by the present invention or at least part of nucleotide sequence can express by suitable expression system to obtain corresponding enzyme or other higher biological activity or output in foreign host.These foreign host comprise streptomycete, pseudomonas, intestinal bacteria, genus bacillus, yeast, plant and animal etc.

Aminoacid sequence provided by the present invention can be used for separating needed albumen and can be used for the preparation of antibody.

The polypeptide that comprises aminoacid sequence provided by the present invention or at least part of sequence may still have biological activity that new biologic activity is even arranged after removing or substituting some amino acid, has perhaps improved output or has optimized the albumen dynamic characteristic or other character of being devoted to obtain.

Their functions in host's metabolic chain of DNA chip technology understanding can be expressed and pass through to the gene or the gene cluster that comprise nucleotide sequence provided by the present invention or at least part of nucleotide sequence in heterologous host.

The gene or the gene cluster that comprise nucleotide sequence provided by the present invention or at least part of nucleotide sequence can come construction recombination plasmid to obtain its biosynthetic pathway by genetic recombination, also can and then obtain its biosynthetic pathway by insertion, displacement, disappearance or inactivation.

The clone gene or the DNA fragmentation that comprise nucleotide sequence provided by the present invention or at least part of nucleotide sequence can obtain new SFA analog by interrupting biosynthetic one or several step of SFA.Comprise the output that DNA fragmentation or gene can be used for improving the SFA or derivatives thereof, the invention provides the approach that improves output in genetically engineered microorganism.

Comprise the poly-peptide synthetase of non-ribosomal provided by the present invention and can produce new poly-peptide compounds by the poly-peptide synthetase structural domain of one or more non-ribosomals, module or the gene that disappearance, insertion or inactivation come from the poly-peptide synthetase of identical or different non-ribosomal system.

Comprise polyketide synthases provided by the present invention and can produce new polyketide by one or more polyketide synthases structural domains, module or the gene that disappearance, insertion or inactivation come from identical or different polyketide synthases system.

The fragment or the gene that comprise nucleotide sequence provided by the present invention or at least part of nucleotide sequence can be used for building the poly-peptide synthetase storehouse of non-ribosomal or the derivative storehouse of the poly-peptide synthetase of non-ribosomal or combinatorial libraries.

The fragment or the gene that comprise nucleotide sequence provided by the present invention or at least part of nucleotide sequence can be used for building polyketide synthases storehouse or the derivative storehouse of polyketide synthases or combinatorial libraries.

In a word, all relevant genes of SFA biosynthesizing and the albumen information of comprising provided by the present invention can help people to understand the biosynthesizing mechanism of SFA class natural product, for further genetic modification provides material and knowledge.Gene provided by the present invention and protein thereof also can be used for seeking and find can be used for medicine, industry or agriculture compound or gene, albumen.

Description of drawings

Fig. 1: the chemical structure of SFA

Fig. 2: the gene structure of SFA biological synthesis gene cluster and restriction mapping.(A) 4 overlapping clays have represented the DNA zone of faint yellow streptomyces gene group 150kb, BamHI represents restriction enzyme BamHI, vertical line on DNA fragmentation represents the BamHI restriction enzyme site, and entity represents that by the part of DNA sequencing, Probe-P1-P4 represents the probe portion of mark; (B) genomic constitution of SFA biological synthesis gene cluster.

Fig. 3: the biosynthetic pathway of each component units of SFA of proposition.(A) start element; (B) special extension apparatus; (C) pyridazine acid; (D) m-tyrosine

Fig. 4: the SFA skeleton forms approach

Fig. 5: SFA biological synthesis gene cluster clone probe sequence interrupts high performance liquid chromatography (HPLC) the analysis A:WT of tunning; B:Standard; C:Mutant

Fig. 6: the high performance liquid chromatography (HPLC) of gene substitution mutant strain tunning is analyzed (A) wild-type (B) zinc in conjunction with dehydrogenase gene sfaJ knockout mutant strain (C) Phenylalanine Hydroxylase Gene sfaA knockout mutant strain.

Nomenclature

Fig. 1 Sanglifehrin A, B, C, D, E: SFA A, B, C, D, E

The gene of Fig. 2 Non-proteingenic non-coding dietary protein origin; The NRPS non-ribosomal gathers the peptide synthetase gene; PKS polyketide synthases gene; Start unit start element gene Extending unit extension apparatus gene Others other.Letter b represents the BamHI restriction enzyme site

Fig. 3 marks the albumen of the coded by said gene in each gene cluster of describing in corresponding this specification sheets of X in the step of SfaX; The step of mark question mark represents to know whether by which enzyme of this gene cluster genes encoding completed or completed by the enzyme of primary metabolite in vivo.

Fig. 4 Module: module; PKS: polyketide synthases; NRPS: NRPS114; KS: ketone group complex functionality territory; AT: acyl group forwarding function territory; KR: ketone group restoring function territory; ER: enol restoring function territory; DH: dehydrating function territory; ACP: ester ACP; C: peptide condensation functional domain; A: adenosine functional domain; PCP: peptide ACP

Fig. 5 WT wild-type Standard SFA A standard substance Mutant mutant strain, arrow indication SFA A

Fig. 6 arrow indication SFA A goes out the peak position

Embodiment

The invention will be further described below in conjunction with accompanying drawing

Crotonyl-CoA reductase gene segment clone in 1 SFA gene cluster:

Based on the analysis to current large ring skeleton PKS-NRPS hybrid structure, may have long linear PKS-NRPS zone in this gene cluster, there is no specificity with the PKS and the NRPS that extensively distribute in the streptomyces gene group, therefore may increase clone's difficulty.Therefore the inventor attempts using other specific unit to carry out the clone of gene cluster as probe.Analyze by the polyketone natural product biological synthesis gene cluster that occurring in nature is contained ethyl structure, the inventor finds that the ethyl unit all introduced as building block by ethyl malonyl-list acyl coenzyme A.The inventor infers that the ethyl unit in SFA derives from this approach equally.Therefore, design degenerate CCR primer is cloned the responsible ethyl unit biosynthetic crotonyl-CoA synthetic enzyme high conservative zone sequence that may exist in the S.flaveolus genome and it is labeled as probe and carries out library screening, degenerate primer sequence C CR Long-For:AGGAAT TCATGG CCTCCK CSRTSA ACTACA AY, CCRLong-Rev:TCGGAT CCGCCG AAGTTS GTCTCR WABCCC CA; CCR Short-For:AGGAAT TCGACA TCGACA TSGTBW TCGAG CA, CCR Short-Rev:TCGGATCCGATG ATGCGC TTSWSB KDCATC CA amplifies respectively 900bp and 300bp sequence from the SFA genome.Cut glue recovery PCR fragment and cut with EcoRI, BamHI enzyme, then be cloned into the pSP72EcoRI/BamHI site.Recombinant plasmid is cut evaluation through enzyme, and sequencing analysis has been cloned into 3 groups of different CCR gene segments with quite high homology altogether.

The clone of 2 SFA biological synthesis gene clusters, sequential analysis and functional analysis

The above-mentioned segment that is cloned into is carried out digoxigenin labeled carry out respectively library screening.Three groups of glutinous grains that obtain are again divided into groups and according to restriction enzyme mapping, its relative position carried out association, then select the BamHI fragment of pTL3102 left side end group to carry out chromosome walking as probe, obtain glutinous grain pTL3103, then select BamHI fragment step of the leftmost side to move and obtain pTL3105, the left side BamHI fragment of then choosing again pTL3105 is carried out chromosome walking and is obtained pTL3106.Altogether be cloned into the approximately chromosomal DNA of 150kb (Fig. 2) through 3 chromosome walkings like this.Utilize respectively PKS, NRPS degenerated primer that some glutinous grain PCR are detected, specific band has been arrived in the part amplification, confirmed this gene cluster comprise CCR and with the PKS-NRPS heterozygosis.

The functional analysis of table 1 SFA biological synthesis gene cluster

Choosing three the highest glutinous grains of level of coverage checks order entirely, the inventor has obtained 118,372bp continuous nucleotide sequence, bioinformatic analysis shows and has wherein comprised 44 open open reading-frame (ORF)s (open readingframe ORF), infer wherein have at least 20 ORF relevant to the SFA biosynthesizing, comprise 1 NRPS gene (sfaD) and be responsible for the synthetic of large ring three peptide backbones; 5 PKS gene (sfaE～I) complete the synthetic of large ring polyketone skeleton may be responsible for precursor synthetic gene (sfaA-B with 11, sfaJ-R) be responsible for synthesizing and backbone modification of each condensation unit of initial sum, 2 regulatory genes (sfaC and sfaS) participate in the biosynthetic regulation and control of SFA, and a unknown gene sfaV1 is responsible for unknown function.Each gene function analysis the results are shown in Table 1.

3 SFA biological synthesis gene cluster borders are determined

The inventor has inferred the border of SFA biological synthesis gene cluster.Left border roughly is positioned at the sfaA upstream, comprises two unknown function genes (sfaU1 and sfaU2).Right side boundary roughly is positioned at MbtH gene three, downstream unknown function gene and two transposase genes (sfaV1～3).The MbtH family protein is common and NRPS is chain, the inventor infers that it is relevant to the start element biosynthetic controlling, but this family protein has the trans-complementation characteristic, namely only have inner all MbtH of full genome to knock out when concentrating on a mutant and just reflect its function, otherwise it is complementary to be positioned at the corresponding protein institute of genome other positions.Because the total quantity of MbtH in Host Strains is unknown, therefore select downstream agnoprotein and transposon to analyze.

1) left border research

Left border research concentrates on sfaU1 and sfaU2.SfaU1 total length 453bp, the Unknown Function albumen of 150 amino-acid residues of coding has 60% homology with agnoprotein in this dimension streptomycete (Streptomyces sviceus); SfaU2 total length 468bp, the Unknown Function albumen of 155 amino-acid residues of coding, dissociate by analysis with in a kind of streptomycete (Streptomyces turgidiscabies)/intergrase has 81% homology.The sfaU1 gene knockout is confirmed that itself and SFA biosynthesizing have nothing to do.Confirmed at least sfaU1 with and the left side gene should be relevant in the SFA biosynthesizing.

2) right side boundary research

Right side boundary research concentrates on sfaV1, sfaV2 and sfaV3.SfaV1 total length 537bp, the agnoprotein of 178 amino-acid residues of coding has 81% homology with agnoprotein in livestock streptomycete (Streptomyces cattleya); SfaV2 total length 459bp, the unknown function gene of 152 amino-acid residues of coding has 46% homology with unknown function gene in streptomyces coelicolor (Streptomyces coelicolor); SfaV3 total length 435bp, the agnoprotein of 144 amino-acid residues of coding has lower portion homologous with IS-4 insertion sequence in Vietnam's bulkholderia cepasea (Burkholderiavietnamiensis).We think that the right side boundary of SFA gene cluster should be at the sfaV1 in sfaV3 and left side, between sfaV2 for this reason.

The biosynthesizing of 4 SFA start elements

the acetoacetyl-CoA that derives from body refers under the reductase enzyme effect that at the zinc of sfaJ coding reduction obtains hydroxybutyryl coenzyme A and then obtains crotonyl-CoA under the short chain dehydration/reductase enzyme effect of sfa coding, crotonyl-CoA reductase enzyme reduction carboxylation through the sfaR coding, complete at initial acyl carrier protein (by the sfaE-ACP1 coding) extension that is integrated into follow-up two carbon units under acylation after the class aspartic acid synthetic enzyme ammonification of sfaP coding, other unit that while sfaL coding is responsible for mistake on acidylate with the free acyltransferase of rhetorical function are hydrolyzed.

The biosynthesizing of position tyrosine between 5 SFAs

Conventional phenylalanine/tyrosine/tryptophane hydroxylation system all relies on electrophilic substitution reaction in the electron rich zone of aroma system, hydroxylation to occur, and position between the phenylalanine of relative light current is dropped in a phenylalanine hydroxylation position, position.The second adenosine functional domain (A2) bioinformatic analysis in NRPS is shown, be responsible for the adenosine functional domain fingerprint region of identification phenylalanine in its active fingerprint region and other systems without any homology, related with tyrosine also not obvious.In vitro study confirms that its coded product SfaA is to free phenylalanine and non-activity, but its mucolyticum acid amide (SNAC) thioes derivatives is but shown activity, show that phenylalanine is the suitableeest substrate of poly-peptide backbone integration, tyrosine unit, position between after namely integrating, the online modification of generation obtains.

The biosynthesizing of 6 SFA pyridazine acid units

The 3rd building block of the short-and-medium peptide moiety of SFA structure is to participate in 2,3-regioselectivity the pyridazine acid unit that poly-peptide backbone is integrated.Originally thinking may be through the proline(Pro) precursor through 1,2-position dehydrogenation, ammonia solution, N ⁵Be oxidized to encircle to restore and obtain; The real precursor of the labelling experiment proof pyridazine acid unit of reference subsequently is L-glutamic acid, infers that therefore L-glutamic acid/glutamine is that precursor is through hydrogenation dehydration, N ⁵Oxidation and hydrogenation form.Certainly, although do not obtain direct evidence take ornithine as precursor in the labelling experiment of other systems, the inventor thinks that still it may directly become ring to participate in the formation of pyridazine acid in this system after ornithine N5 oxidation.

The biosynthesizing of 7 special extension apparatus

The reusable polyketide synthases of SfaK coding is considered to participate in the biosynthesizing of special extension apparatus six carbosilane units.Acetyl-CoA or malonyl-list acyl coenzyme A are that start element obtains poly-triketone caproic acid coenzyme A through two-wheeled two carbosilane units extensions, then the ketone group that the mid-way is removed in the special redox functional domain selective reduction that has through this enzyme end obtains the conjugated double bond coenzyme A derivative of partial reduction, and then the crotonyl-CoA reductase enzyme reduction carboxylation activation of accepting the sfaR coding is incorporated in polyketone chain extension process.Simultaneously, the generation of SFA is not found in the mutant strain fermentation that gene is knocked out gained, has confirmed thus this gene and SFA biosynthesizing dependency.

Enforcement embodiment below further is provided, and these are implemented embodiment and help to understand the present invention, only do not limit range of application of the present invention with explaining.

The extraction that embodiment 1 SFA produces the total DNA of bacterium faint yellow streptomycete Streptomyces flaveolus

1. the S.flaveolus A92-309110 spore suspension with-80 ℃ of refrigerations is inoculated in 3mL YEME substratum, 30 ℃ of incubations are cultivated 12h, be transferred at twice centrifugal recovery thalline in the 1.5mLEP pipe, and with 500 μ LSTE solution suspension thalline, and add N,O-Diacetylmuramidase to final concentration 4mg/mL, 37 ℃ of lower water-bath 30-45min.

2. when thalline becomes the transparent colloid shape, add 250 μ l 2%SDS, 60 μ L 5mol/L KAc and vibration mix, be frozen in-20 ℃ after 10 minutes 12000 rev/mins centrifugal 10 minutes, shift supernatant in new EP pipe.

3. add the 500 isopyknic phenol/chloroform mixtures of μ L, 10000 rev/mins of centrifugal 3min, repeat to occur to no longer including at the interface white metaprotein several times, again with the independent extracting of chloroform once, pipette supernatant and add the equal-volume Virahol to mix under rear normal temperature and place 30min, 10000 rev/mins of centrifugal 5min, drain after 70% washing with alcohol add 200 μ LTE damping fluids and 10 μ L10mg/mL without the RNA enzyme of DNA enzyme in-20 ℃ of freezing preservations.

Embodiment 2 SFAs produce the foundation of the faint yellow streptomycete Streptomyces of bacterium flaveolus genetic transfer system

1. at first the target plasmid with transfer to be joined is transformed in intestinal bacteria E.coli S17-1, and on the picking flat board, mono-clonal is inoculated into 37 ℃ of incubations that spend the night in the test tube that contains 3mLLB substratum and corresponding selective pressure.Second day results bacterium liquid is drawn 1mL bacterium liquid and is inoculated in the 250mL Erlenmeyer flask that contains 50mLLB substratum and corresponding selective pressure 37 ℃ of shaking tables and is incubated to the OD600 value and reaches 0.5 left and right.Shift bacterium liquid and reclaim thalline at 16 ℃ of centrifugal bacterium liquid of lower 3800 rev/mins of 10min in the 50mLEP pipe and then add 20mLLB substratum washed twice and the centrifugal recovery thalline of similarity condition again, 1mLLB is resuspended, and is stand-by.

2. get the centrifugal 3min removal of 12000 rev/mins of room temperatures of the spore suspension one pipe supernatant liquor that very low temperature is preserved.Resuspended with 1mLTES damping fluid washed twice 500 μ LTES damping fluids, heat-shocked 10min under 50 ℃ of water-baths, then added 500 μ LTSB substratum mixing 37 ℃ of incubation 4-5 hours, the whole clear liquids of centrifugal removal add 1.5mLLB resuspended stand-by again.

3. get each 100 μ L of recipient bacterium and donor bacterium and be coated on the MS substratum flat board of (containing 10mM MgCl2), cultivate 12-16h for 30 ℃, and get a plate and only be coated with recipient bacterium as negative control.Cultivate later that every block of plate adds the distilled water of 4-5mL sterilization and gently scrapes the surface with push rod, blot after water maximum wind speed and blow 1h and add a cover again 1mL sterilization distilled water (containing 12.5 μ g/mLAm and 50 μ g/mLNA) and cultivated 3-5 days under 30 ℃.

4. will transform the mono-clonal picking that successfully grows and be inoculated in the 3mLTSB substratum 30 ℃ and shake bacterium, and get 1 μ L left and right bacterium liquid after 1d and be coated on 150mm and contain on AmR ISP-4 massive plate and cultivate 2d under 37 ℃, with containing Tsr ^RCultivating 6-7h under the ISP-4 plate culture medium directly covers 37 ℃ then separates two substratum again, cultivate again respectively 2d under 37 ℃, the mono-clonal that grows on picking AmR but not growing monoclonal colony inoculation in the TsrR corresponding position shakes bacterium in the 3mLTSB substratum, prepare fermentation.

Embodiment 3 SFAs produce the foundation a small amount of enzyme of the faint yellow streptomycete Streptomyces of bacterium flaveolus gene library and conscientiously test

1. at first determine the consumption of Sau3AI by a series of dilution experiment, the reaction system that first prepares 250 μ l (contains genomic dna 40 μ l, BSA (100 *) 2.5 μ l), then 250 μ l reaction systems are divided into 1 * 50 μ l and 7 * 25 μ l and are placed on ice.Then get the Sau3AI that 2 μ l are diluted to 0.5u/ μ l in advance.Add in the reaction system of the first pipe 50 μ l, mix in rear transferase 12 5 μ l to the second pipe 25 μ l, repeat such transfer step 7 times.70 ℃ of inactivation 10min after 37 ℃ of water-bath 15min of all systems, 4 ℃ of cold house's 0.4% agarose gel electrophoresis detect the quality that enzyme is cut under ultraviolet lamp or under the gel imaging instrument after ethidium bromide staining.

A large amount of enzymes are tested conscientiously

2. reaction conditions determined according to preliminary experiment prepares the amount of total DNA of 4 times and isocyatic enzyme dosage and builds the required DNA fragmentation in library: system at mixing on ice, is equally divided into five parts, 37 ℃ of water-baths are respectively at 12,14,16,18,20min takes out and is placed in 70 ℃ of inactivation 10min.Through 0.4% agarose gel electrophoresis, detect the quality that enzyme is cut after ethidium bromide staining under ultraviolet lamp or under the gel imaging instrument in 4 ℃ of cold houses.

Dephosphorization

3. enzyme is cut sizeable DNA fragmentation and use successively saturated phenol, the extracting of chloroform isoamyl alcohol solution adds the 3M NaAc of 0.1 volume, the dehydrated alcohol precipitation of 3 times of volumes, and 70% washing with alcohol is dissolved in 200 μ l water after draining.Take out 10 μ l and give over to contrast.Remaining carries out dephosphorization, and the DNA that 190 μ l Sau3AI enzymes are cut adds 10 μ l SAP (Promega, 1unit/ μ l), and 10 * damping fluid, 25 μ l, and water complements to 250 μ l.System was bathed 1 hour 37 ℃ of temperature, added 7 μ l SAP mixings, and then temperature was bathed after one hour, and 70 ℃ of heat denatured make enzyme deactivation.Use successively saturated phenol, the extracting of chloroform isoamyl alcohol solution adds the 3M NaAc of 0.1 volume, the dehydrated alcohol precipitation DNA of 3 times of volumes, and 70% ethanol is washed, and is dissolved in 15 μ l TE solution after draining.Whether the joint detection dephosphorization is complete.4 ℃ of cold house's 0.3% agarose gel electrophoresis detect the quality that enzyme is cut under ultraviolet lamp or under the gel imaging instrument after ethidium bromide staining.

The preparation of SuperCos1 carrier

1. at first use XbaI single endonuclease digestion SuperCos1, make it be broken into linearity between two cos sites, dephosphorization (preventing from connecting) then, then cut with the BamHI enzyme, obtaining 1kb, two arms of 7kb are so that the genomic DNA fragment that the insertion portion enzyme is cut.The concentration of the carrier for preparing is 1 μ g/ μ l.

Total DNA segment is connected with the SuperCos1 carrier

2. the SuperCos1 (approximately 1 μ g/ μ l) that DNA (approximately 2 μ g/ μ l) and the 4 μ l of 6 μ l dephosphorization treatment is prepared mixes, and then takes out 1.2 μ l in contrast; Add 1.2 μ l T4 DNA ligases (NEB 400u/ μ l) and 1.2 μ l damping fluids in 8.8 remaining μ l mixed solutions, 16 ℃ of connections of mixing 16 hours, 4 ℃ of cold house's 0.3% agarose gel electrophoresis detect under ultraviolet lamp or under the gel imaging instrument after ethidium bromide staining.

The library packing

3. take out the Gig Pack III XL packing reagent of a Stratagene from-80 ℃ of refrigerators, clamping speed with finger tip melts, adding 4 μ l to connect products blows and beats gently three times (avoiding bubble) with rifle and puts into 22 ℃ of water-bath 2hr and take out and add 500 μ l SM damping fluids, put upside down several add after lower 50 μ l chloroforms put upside down several lower after (at this moment can see some albumen shapes precipitations) be placed on and get rid of on whizzer, shift supernatant with rifle, be put in 4 ℃.

Titration

4. first the E.coli VCS257 on dull and stereotyped son is chosen single colony inoculation incubated overnight in LB, get 500 μ l bacterium liquid and be forwarded in 50ml LB (10mM MgSO4,0.2% maltose), 37 ℃ of shaking tables are cultivated, intestinal bacteria OD ₆₀₀Reach 0.84.Taking out 5 μ l packing liquid adds 45 μ l SM damping fluids to add 50 μ l E.coli VCS257 to dial gently to be placed on 22 ℃ of water-bath 30min after even again, taking-up add 400 μ l LB to put upside down to put into several times 37 ℃ of water-bath 75min (every put upside down in 15 minutes several under), each is drawn 250 μ l and is applied to that on the LB flat board, (Amp100 μ g/ml) is put in 37 ℃ of incubated overnight.

Detect the library verity

5. false-positive in order to prove that chief bacterium gets blamed, but really contain the clone of recombinant cosmid, random picking 10 clones receive in LB (Amp100 μ g/ml) and cultivate.Alkaline process a small amount of preparation method according to e. coli plasmid dna extracts glutinous grain, and the BamHI enzyme is cut, electrophoresis on 0.5% agar gel.

Amplified library

1. E.coli VCS257 is received 50ml LB and (contain MgSO ₄10mM, maltose 0.2%) be cultured to=carried out at once transfection at 0.84 o'clock, bacterium liquid (100 μ l) and packing liquid (100 μ l) gently mixing put into 22 ℃ of water-bath 30min and backward 5 little centrifuge tubes add respectively 800 μ l LB gently mixing be put in during 37 ℃ of water-bath 75min that mixing is once gently every 15min.Simultaneously 5 blocks of massive plates (Amp concentration is 100 μ g/ml) are put into 37 ℃ of incubators in water-bath, warming by fire and; Carry out coated plate after water-bath is complete, a little centrifuge tube is coated with a plate, puts into 37 ℃ of incubator incubated overnight.By the time after having cultivated 18hr, (bacterium colony similar large time) adds 3～4mlLB then with hairclipper, bacterium colony to be scraped then on the every plank with rifle, bacterium liquid to be transferred to the 50ml centrifuge tube again, the bacterium liquid of 5 large planks is merged to add Amp and aseptic glycerine final concentration be 50 μ g/ml and 18% (V/V), be distributed into the 250 every pipes of μ l and be put in-80 ℃ of preservations.

Embodiment 4 SFAs produce the faint yellow streptomycete Streptomyces of bacterium flaveolus fermentation, product separation Purification and Characterization

Liquid fermenting

1. the bacterial classification of getting targeted mutagenesis bacterial strain mono-clonal or preservation is inoculated into to spend the night in the 3mLTSB substratum that contains corresponding selective pressure and shakes bacterium and cultivate, and gets gained bacterium liquid 500 μ L and again is inoculated into and carries out 27 ℃ of primary fermentations in seed culture medium and cultivate 1d.

2. the primary fermentation bacterium liquid 5mL that will cultivate a day again is inoculated into 25 ℃ of cultivation 1d of main fermentation tank and carries out second order fermentation.Then the XAD-16 resin that will sterilize in advance (final working concentration 4%4g/100mL) joins in main fermentation tank and continues to cultivate 3d and carry out three grade fermemtation under 25 ℃.

After fermentation ends to whole fermented liquid suction filtrations, leach thing and together transfer to drain in-80 ℃ of freezing 30min in beaker together with filter paper (gauze) and spend the night.Collecting whole resin particles adds the 80-100mL anhydrous methanol to stir 40min, collect resin particle after suction filtration and repeat again said process washing suction filtration, discarding resin merging washings steams in 35-37 ℃ of backspin, twice of the solid that adds 750 μ L methanol wash bottle walls bondings after evaporate to dryness, merging twice washings transfers in the 1.5mLEP pipe at-20 ℃ of freezing 1h, 4 ℃ lower 12000 rev/mins centrifugal 10 minutes, with supernatant liquor be transferred in new EP pipe vacuum centrifuge centrifugal to liquid residue 500 μ L left and right 4 ℃ deposit.

4. the fermentation broth coarse extract of no more than 300 μ L is concentrated after rapid column chromatography (RP-18, the pre-drip washing 40mL of 40%-45% acetonitrile discards 50% acetonitrile drip washing 40mL can be in charge of collection) purifying.

5. enter the HPLC isolation identification through pretreated sample.

Solid fermentation:

1. getting 40 μ L spore liquid is inoculated in a large number in the 3mLTSB substratum and draws 400-500 μ L after 37 ℃ of incubations that spend the night and be uniformly coated on the 150mm massive plate 30 ℃ and cultivate and got final product in 6-7 days.The purpose of this step is to utilize solid medium for a long time bacterial strain to be cultivated and makes its generation SFA.Can shorten the streptomycete incubation time with inoculation with a large amount of inoculations of spore.Spore liquid directly can certainly be coated on and make its sprouting on flat board.The selection inventor about substratum has attempted R2YE, IWL-4, ISP-4 and AS-1.

2. the complete plate culture medium that will ferment is recovered in the 250mL beaker after chopping every and adds 200mL methyl alcohol to stir the 2h suction filtration to discard solid and steam filtrate is outstanding, transfers in the 1.5mLEP pipe after adding 1mL methanol wash bottle wall (500 μ L wash at twice).The purpose of this step is to reclaim tunning.The recovery of precaution product during with liquid fermenting.Replenish: less in view of solid fermentation substratum moisture, can save the freezing step of draining.Steam moisture and can't hang to do and to connect oil pump and drain again with a small amount of methanol wash bottle wall collection if revolve after methyl alcohol soaks.

3.HPLC identify (operating the same).The characteristics of solid fermentation are that background knows that this is that the inventor determines to adopt this method to carry out the mutant strain fermentation, can see the generation of new compound so clearly.

High performance liquid chromatograph for the identification of or identify/separate the mixture that is dissolved in liquid solvent or is in a liquid state under (preparative chromatography) normal pressure and temperature.The use rules are as follows:

1. eluent pre-treatment: distilled water, after each 1L filtration sterilization of trifluoroacetic acid aqueous solution (drip washing with) and chromatographically pure anhydrous methanol (cleaning use, SILVER REAGENT also can), exact connect ion is to the chromatographic instrument pump.

2. connect post: will separate chromatographic column used and be installed to correct position.

3. open successively sampling pressure pump, mixing pump and separate pump switch (instrument is arranged usually from top to bottom) and move workstation.

4. set and draw sample 20 μ L with syringe after the drip washing condition and inject injection ports, screw off the sample introduction switch and treat to occur on screen red line and represent that retention time begins timing.

5. gather data is preserved.

Embodiment 5 polymerase chain reaction clone's SFA biosynthesis genes

The PCR system comprises: and DMSO (8%, v/v), MgCl ₂(25mM), dNTP (2.5mM), degenerate primer (40mM), Taq archaeal dna polymerase (2.5u) and the total DNA of appropriate template Streptomyces flaveolus.At first 95 ℃, 3min, 1 takes turns; Then 94 ℃, 1min, 68 ℃, 1min, 72 ℃, 2min, 5 take turns; 94 ℃, 1min, 65 ℃, 1min, 72 ℃, 2min, 30 take turns; Last 72 ℃, 10min, 1 takes turns.After PCR finishes, 1% agarose electrophoresis check result.Low melting point glue reclaims the DNA fragmentation of expection size, be connected with pGEMT Easy vector, transform the bacillus coli DH 5 alpha competent cell, be coated on and contain penbritin, carry out blue hickie screening on the LB flat board of IPTG (isopropylthio-β-D-galactoside) and X-gal (5-bromo-4-chloro-3-indoles-β-D-galactoside).Picking white colony incubated overnight, extracting plasmid, EcoRI enzyme are cut the DNA Insert Fragment whether evaluation contains the expection size.Be inserted with the plasmid order-checking of the big or small DNA fragmentation of expection.

Embodiment 6 making nucleic acid molecular hybridizations

1. mutant strain to be identified is counted the 220rpm concussion in the 3mLTSB substratum of microlitre mycelia or spore inoculating, be cultured to bacterium liquid at 30 ℃ dense, extract total genome.

2. according to sequential analysis design hybridization strategy, prepare probe and select suitable restriction enzyme site digestion genome.

3. get in 15 μ L probes (containing 0.5-3 μ gDNA) boiling water bath and heat 10min, changing immediately cryosel over to bathes cooling, add successively Hexanucleotide Mix (10*) 2 μ L, dNTP Labeled Mix 2 μ L, KlenowEnzyme Labeled 1 μ L mixes rear 37 ℃ of water-bath 16h.Add 0.8 μ L 0.8M EDTA (pH=8.0) with termination reaction, add 2.5 μ L 4M LiCl to mix, dehydrated alcohol precipitation with 75 μ L precoolings,-80 ℃ of freezing 40min, 4 ℃ of centrifugal 20min of lower 12000rpm collect DNA, 70% pre-cooled ethanol washing is dissolved in 4 ℃ of preservations of 50 μ LTE damping fluids after vacuum-drying.

4. electrophoresis gained sepharose is soaked in 20min in 0.25M HCl, then soak 20min with adding ealkaline buffer to shake gently after deionized water rinsing, change liquid and once continue to soak 20min, with deionized water wash for several times after transfer DNA to nylon membrane and be fixed, shady and cool place preserves stand-by.

5. the nylon membrane that will fix is packed into and is added in hybrid pipe hybridization solution to carry out prehybridization 30min in 64 ℃, then adds probe to hybridize 16h at the needs temperature.

6. tight wash-out is at room temperature used the each 5min of the tight elution buffer washed twice of 2*SSC successively, then uses the each 15min of the tight elution buffer washed twice of 0.5*SSC under 64 ℃.

7. first the nylon membrane after tight wash-out is used elution buffer balance 1-5min when developing the color, lock solution 30min, antibody-solutions 30min, 1-2 rear damping fluid balance 2-5min that detects of elution buffer washing, add the dark place colour developing of colour developing damping fluid, wash the hybridization of film termination reaction with deionized water and finish when concentration is suitable.

The following gene that content provides according to the present invention and protein sequence:

Nucleotide/aminoacid sequence table:

SEQUENCE LISTING

＜110〉Shanghai Organic Chemistry Institute, Chinese Academy of Sciences

＜120〉a kind of biological synthesis gene cluster of SFA

＜130〉specification sheets claims

<150>CN200810200388.4

<151>2008-09-24

<160>1

<170>Patent In Version 3.3

<210>1

<211>118372

<212>DNA

＜213〉faint yellow streptomycete Streptomyces flaveolus

<400>1

agcacgcgga gaacgcccgc ctgcgggcgg cggggagagc cgttccggtc caactccccg 60

gcggtgtgcg ggcgtgggcg attccgcacc acgaggacct gcgccgagtg ctgacggatc 120

ccagggtcgc caagggaatc acgcactggg aggcagcggc ccgcggcgaa gtgccggacg 180

ggtggccgtt gatgggcttc gtcgcgaccg acagcgtcat caattcgcac ggcgcggatc 240

accggcggct gcgcggactg gtcgaccagg cactgacccc ggaacgggtg gaggccatgc 300

gcccgggggt ggaacgcctc gtcgacgggc tcctcgacca gctcgccaca cggccggaag 360

accggccggt ggatctccgc aaggctttcg cctaccccat ccccaccacc gtcatctcgg 420

acctgctggg gatcccgcag cgcaagcggc gtctgctgca cgtgctcacc ggcatgcaga 480

cccgtacgaa caactccccc gaacaggtcc aggagatcga cggacggatc gaagcgctgc 540

tccgggagat cgtcgccgaa cgtcgcgacg tcccccggga cgacctcatc agcgtcctgc 600

tcacggcacg ccaggacgga gacgaccggc tcaccgacag cgaactgcac ggcatgatcc 660

tgctgatgtt cttcgccggc caccagagcg tcatcaacgt actcgtcaac gcctgccacg 720

cgctgctcac ccaccccgag caactggcct ccgcccgcgc cggggaggtc ccgtggagtg 780

cggtggtcga ggagacgatg cgctggaacg gcgcggtgaa ccagttcccg atgcgctacc 840

cgaccgagga cgtcgtcatc gccggccaga cgatccaccg gggagaagcc atcctcgcgt 900

ccttcggctc ggcgggccgg gacccggcgc accacgggcc ggacgcggac ctcttcgacg 960

tccgccggcg gcaggccggc cacctcggct tcgggcacgg cccgcacttc tgtatcggga 1020

cacacctggc ccgtctccag ctggagacgg cactggccgg attcttcacc cggttccccg 1080

acgtgcggct ggccccgtcc gacacgtgga ccgtgcggcc cgtgccctcg ttcgtcagca 1140

acagcatcga ggcgctgccc gtactcctcg gccctgccgc ggagtacggg cggctctgac 1200

gtcgcggcac gcacgtgtta cggagccgag ccccacacaa cgcgcggcaa ggtgctcgcc 1260

gacggcctgc gcagaccgac agagaggacg ccctccgtgc ccccgcacac catcgccctg 1320

ctggcaccca ccaccgcagt ctcccccgcc gaaaggctca ccgtccccgc cttcggcgac 1380

gtcaccgtgg accggccggc acacgccgac tggcaccgct acctgcacga gttcgaaccg 1440

tcatccgagc tcgcggcggt gtggatcggg gcggacgccg agccgctgcc cgccgtccgg 1500

gcgctgcggt cgcactcgaa gttcaaccgg acccgggtgt acgtcgtcgg gcccgatacg 1560

gcgggcccgg ttcccggctg ggcggaagca ctggacgtcg aggacatcgt ccggcccgcc 1620

gcggtggcgc ccgaagggtt caccacctcg gtcgaactgg ccctgcgggc gcaccgcgcg 1680

ctgatgacgg acgacctcta caccgactac tacctcgaca tgaccttcaa caagatcttc 1740

gactggttcg agacgacgcg ctgggactgg accgaggtgg acctggaccg catcgagcac 1800

ggcatgctcg acgaacggac cgtggacttc ctcaccgagg cggccgtcat cgagttcggc 1860

accctacccg gcgctcacaa cttcctgcgg gaatggcagg acgaggtcag cttctcctcc 1920

tgggcactgc aatggggtgc ggaggaatcc cggcactcac tggtccaggc ccgctacctg 1980

gaccggatcg gggtctcact ccgttcgaag cacgcgctct acaaacgaga gccgtatccg 2040

cagggcgacg tgcgctccgc caccctcatg atgaacgtga tctcggagtc acgcgcgtcc 2100

gccctctaca aggcgctcgc cgcccatgtg tcggagccgg tgatccgcag aatctggcgg 2160

ctcctggccc gcgacgaggc ccggcactgc cgtgcgttct ccgtcttcat gcgggaactg 2220

tgcgacagcg atccggccca tcaggccgcc gcgctgcgca tggcctacat ctggctcgcg 2280

gaccgtagca acggcgtcaa gcacccggcg ggcatgttct acccgcattc cacatccacg 2340

gccggcatcc gccgtatcga gaccatccag cacgactcgg tggacgccgc ggacgccaag 2400

gtgatgtcga tcgtccggct cctggcggac gacgactcgt tggagacccc gcgggacatc 2460

aaagccaagc tgcgcgccct ggcccgccgg cccggcgctc gctgaccgtc agtcccggcg 2520

tccgcctccc gccgcgcccc gacgcgccgg cctcacagaa caggacaaca ccacgccatg 2580

tcacaggcga gtgtcaccga catcgaacag accgtccgcc gatccgtcgc cgagcaactg 2640

cgcatcggtg tcgaggaggt gggcgtggac accgatctgc gctccttgcc gcgcttcgac 2700

tcgatccacg cgctccagat cgtgctggag atcgagcagc actacgacat cgaggtggag 2760

gaccgcatcg tcttcgagac gagcaccgtg cgggagttcg cggccgcgat caccaaggtg 2820

atcgcggagg ccggcacgga accgctgtcg tgaccctgtt cgccgcgctc ggccgcgtcg 2880

ccgcacaagg ctccggacgg ggcatgcacg tgctgcgccg gggccaggac ggcgacgcgc 2940

tgtcctacag cgacctcttc gccgaggccg ggcgggtggc cgcggggctg ctgtcgcgcg 3000

gggtgcgacc cggcgagcgg gtcgccctcg tcctgccgac gtcggtggac ttcgcgcgcg 3060

ccttcttcgg cgtgctggcg gcgggcgcgg tcgcggtgcc gctgcccggt cccgcgccct 3120

tcgggtcgtc cgacgcctat ctccggcgca cctccgcggc cctgcgccgc tctcgggtac 3180

ggaccgtgct gacggctccg gccatgcgcc cgctgctcgg tcctggactg tccgacggcg 3240

cccgcccggt cgacgtactc ctggtgccgg aggtggccga gccgtccgcg gcccacgtcg 3300

cgcgggcggc gtcggacccg gccgtcgttc agtacacgtc gggcacgagc agcgaaccgc 3360

ggggcgtcgt gctcagccac ggcaacgtgg cggccggggt ggcggccatc gcccatggca 3420

cacggctcgc caggaccgac gtcggctgca cctggctgcc cctcttccac gacatgggcc 3480

tgatcggttc cttcttgaca ccgctgctgc acgacgtcga catccatctg ctgacaccgg 3540

aggactacct gcgcagtccg ggtgactgga tccgggccat ggggcggctg ggggcgacct 3600

tcaccatggc tcctgactcc ggctaccgct acgtcctgag gcgcgacgcg gcgccgccgg 3660

ccggcctcga tctctcccgc tggcgcatcg cggtgaacgg cgccgagccg gtcgaccggc 3720

ggcttcagga cgccttcgcc gagcgtttcg cgcccgccgg actgtccgag aacgtcttcc 3780

tgccggccta cggactggcg gaggcgaccc tggcggtcgc cttccctccc ctcggccgcc 3840

cggccaaggt gctgcgtgcc gaccgggacg aactgaaccg gggccgttac gtaccggtgc 3900

cgtccggcgc cgggccgtgt cgcgaactgg tcagtgtggg aacgccggtg cggcgtaccg 3960

aggtccggct caccaccgcg agcggcgcac cggccgtgcc cggcacggtc ggcgccgtcg 4020

aggtccgggg ggcgtccgtc acgagcaccg ggtacgaccg ccaccccgaa gagagccgtc 4080

gcgtcctcct tccgggcggc tggttggcga ccggtgacct gggcctgtgg cacgacggcg 4140

agctgtacat cgtgggccgc acgaaagagg tgatcatcgt cttcggcgcg aaccactggg 4200

cgagcgacat cgaggcggtc gtgcgggaca cacccggcct cctcgtccac ggcgtcctgg 4260

ccgagcagat ctggagcgac gagggaggcc gcctcgggct cgtcgtcgag acgacgcggc 4320

acgacgaggc gagccgtcgt gccacgacgc aacacatccg cgcacgtgtg gtggccgagc 4380

tgggcatcac acccgacacg atcgagttcg tgcggcgcgg cgggatcgca cgcacctcca 4440

gcggcaaggc tctgcgccgc ccgccgcctt cggacacgta catgtcccgc tccggtgcgt 4500

gacatacccg gaacaccgga ccacaccgca cggccgctac agggcactca gtgctccaga 4560

caccgcggag ccccctggcc acctgccgtg gggaactccc cgcacgaccg gccctggagg 4620

cctcgcatgg cgcacgacgc atacctgctc ggttactccg atagcgagca ccaaaggctg 4680

acccttcaga acgaggcgat ctcgccgttc accgaacgca ccctgcggtc ggccggagta 4740

cgtccgggga tgagcgtcct cgacatcggt gccggactgg gatacgtctc tctgctggcc 4800

gcccaactgg tcgggcccgg cggacacgtc gtgggaatcg agcgcgaccc cggaaccgtc 4860

cacaaggcgc ggatcctggc acaggaggcg gggctcgcgg actcggtgcg gtacgaagcc 4920

gcggacctgg ccgacttcac ctggccgatg aagttcgacg tcctggtcgg caggtacatc 4980

ctgatgtacc tgcccgatcc cgcatccgca ctgcgccgtc tgacgtccct gctgaaaccg 5040

gacgggatcg tcgtcatgca cgagatggac ttcaccaaca cgtcgccgtc acgtccgccg 5100

ctgccggagt gggacgagta ctacgcgctg tggcccggcc ccttcctggc ggccggcgcc 5160

gtccccgact tcggcccccg cctcacccgg acgttcctcg acgccggcct ccggcagccg 5220

gaagtggcca cctggacgcc ggtcgcgggc cgctcgggag atcccgcggt cctcaactgg 5280

ctcagcacca cctgccgcga gatcgcgacg ccgctgcgca acgccggcgt cacggtgccg 5340

gaccacctcc gctttgacga cgccctgcca cagcgactcc gggacctcgt cctgtcccgc 5400

agagcgcagg tcgacgggcc gacgcagttc ggtgcccacg ctcggctgag accacaggcg 5460

tccaacgcgt caggccgcga cggcgggatg cgctgagagg cagggaacac accgtgcgcg 5520

ctacgtgacg aacatccggc agccggtctg tccgccaaag ccggtctgtc cgccgaagcc 5580

gggcagcccg aacagcgtgc agaccgaaca gcgggccgcc ttccgggcgg tggaaaaagc 5640

ccgctcgccc gacccggccg ccacccgatg ggtcgtctcc cacggccgtc ggcggcaggg 5700

gagacagaca ccccgcggcg tttccgaccg aacaggagta atcgaccatg tccggcactt 5760

tcgatcacca cagggtcggc cccctgccgc ccttcctggc agcgccatca agtgaggccg 5820

tcaaacgggt gcggatgccc actggtgatc acatgtggct ggtcaccgac tacgccgtgg 5880

ggcgcgccgc gctcgccgac acacgtctca gcagagctgc ggccacccac cccgatgctc 5940

cgaaatgggg atccgtcgac ctctcaccca actcgatcat gagtctggac ggtgcggacc 6000

acgcccggct gcgccgggtc gccggagcag cgttcaccag cgcccgagtc accgcacagg 6060

cagcggtgat cgaggaggcc gccgacgcac tgctggatcg gctacaggtt gcggggccag 6120

gcgccgacct ggtcagcggt tatgcttcgc cccttcccat ggtcgcgttg agctcactgc 6180

tcggcgtgcc ggcggcggag cggcccgttt tcgacgccgc cgtgactgcg ctgttcgaca 6240

tgacgcctca gggaacccgc agaagaggcc gccatatcct gactcttatc aagtacatgt 6300

cctcactgat cgaaagaaag agggatgcgc ccgggcagga tctcctgagc gcgctggtgc 6360

gggttgagca gtcgggtgag atatcccgca tcgagttgat caacctcgga ctggcacttc 6420

tcatggccgg ttacgagacc accgcccatc aattgagcct tgccatactc gaactgctca 6480

ccggtgacgc gctcgacggg caatccgtgg aggatctggt tgaggagctg ctccgcaaga 6540

cgccgtccac acctatcagt tttccccgag tggccatcga ggaccttgtc ctgggcggga 6600

cgacggtgcg ccaaggagag gccgtcatcg tctcgttgct gcacggcaat catgacgccg 6660

aagtattttg cacatcgggt gcgaagagca gcgcccgccg gcctgcacac ctcacattcg 6720

gacacggcgc ccaccggtgc atgggcgcgc cactggttct tctccagatg aagatcgcgc 6780

tgacccgctt gtggcagcgc ttccccggcc tgcgactcgc accgggggac gaggccctcg 6840

tatggcggga gggcctcgcg acgcgcggtg tgtcaaggct ggtcgtcgag tggtaaccga 6900

cacgttcggc gtccacacag gctcggctgc tcgctcgggg tcgcggcgtc gttctcctga 6960

tcggtgtacg cgctgttccg gggaggagcg ggcgagcctg gcatgcgggc gatctttcct 7020

tggtcaccgg tgagagtgaa cctgtggagg tcggcgactc cagcgcggct gcggatacgt 7080

gcgtcagtgg cccctgtccc accactccgt cgttccgcgt cgttccgccc acccgctaca 7140

tgatcacctc cgatgatccc gctacccggt gcaccagcgt cttccatcca gggcacgagc 7200

acgtagattc gaacacccct ccgtgccgat atgatgccgc ttcggtcgtc cgacggaggc 7260

caggctcgtc acctgcgcct ggggcgcttc cacgactgcg ctccgcggcg gtgcgtgtgc 7320

cgtggaagag ccggtgcgcg ggaatcgcgc ccgcggacgg tgtcccggga aagaagcgag 7380

gtatggcagt gatctgcgtc ggcggcatga tcgggatcgg gaagacaagc gtggccgagc 7440

tgatcgccaa agagctgggg agcatggtct tctacgagag cgtggaggac aacccgatcc 7500

tgccgctctt ctacaccgcc ggtcctgagg agatagcggc gaagcgctat cccttccttc 7560

tccagctcta cttcttgcag acgcgattcg ccgcgatcaa ggaggcgtac aaacaggacg 7620

acaacgtcct cgaccggtcg atctacgagg actggtactt cgccaaagtc aatcacgacc 7680

tcggcaggat cagttccctg gagatgcagg tgtacgaggg gttgctcgcg gagatgatgc 7740

gtgagatcga cggcctgccc tatcgcaagg cacccgatct catggtgtat ctcagagcgg 7800

acttcgagac ggtgctgcgc cgcatcggac tgcgggggcg tgacttcgag caggaccaaa 7860

gtctcgtcga gtactaccgg acgctttggg ccgggtacga cgactgggtg cacgagcact 7920

attccgccag cgatgtcctc gtgatcgaca tgaaccgcac cgacgtggtg aacagccccg 7980

acgacgcgaa gcgtgtcgtc cgggaggtcg cggacgccct tgccgtggcg aaagtccggc 8040

gcatctgacg ctgcgcggcg atcggtcgcc gaggcggcgc ggcatgcgca gcacggctca 8100

gccgtccgga gacctcgtcg gcacggcagc ggagcagcgt gccgtgaaag ccgccgcatc 8160

caggatcggg ggagcggcgg tcagcgtctt ccgaagtggg acgggtggca caatgaccgc 8220

tgacttggcc gacagcgacc cgaggaaacc ggcgtgaatc cgacgcggca gcgcgactgg 8280

gcctgcccgt caagatggcg ccgcggctgc tcgcgggcaa ctcggcgccc gcgctcatcg 8340

ggtcggctgg gcaactgctg aggtcccggg ccggtctggg acaccgctca ccgagctgac 8400

acgggggatg aagatgaagt cgagcaccgc cgcggccgcg cgtccggcga cggggccgag 8460

catcccctgt ccgggcatcg tgacggtcca ggtggtgtct tcggccagga ccgtttccag 8520

ctcgggtata tgcaactcgt ccaaggcgtg ggcgtagttc gccaccgcct gctggagttt 8580

cggcgatcac gaggccaccg tcagcacgat cttgccttgg atgtggccct gcgcggctcg 8640

cgtgtgtgcg ctgaccgctt cggacagcgg gtaggtgctg tccaccccga cccggagtgt 8700

gccctcgtcg aacaggcgcc cgatctcggc gagctggggg ccgtgggaac gtacctgaat 8760

gttcgagacg gtgatgtcca gactcgccgt ctcttccggg tcgtactcgg cgaagaacac 8820

cgggagcagg gtgccgccgc gcttgagtac ggtcaggaag cgtgagccgt ccgggccgcc 8880

gacggtgtcg atcaccaggt cgacaccgct gaccacgtcc gcggcctgcg tcgtggtgta 8940

gtcgatgaac tcatcggcac cgagctcgcg caggaaccgc tcgtgccgac ttgaggccac 9000

cgcgatgacg tgtgccccct tccatttcgc caattgcacc gcgaaatggc ccactccacc 9060

ggcggccccg ttgaccagca cggtcatccc cggcgtgatc ggcaccggct ggtgcacctg 9120

gccggtgaaa ggagacggca cctcgtggcc gaggtcgacc aggtactgcc aggccgtgag 9180

cacggccatc ggcgccccgg ccgcctgcac gtggtcgata ccggccggct tgtgagccag 9240

gtcagaagcc ggcgcggcca cgtactgggc gtacgtccgg ccgtcgaatc cggggaaccg 9300

cagcatgccg aagacctcgt cgccgacgcc gaaccccggc acgtccggag cgaccgcctg 9360

gaccacgccc gacatgtccg ttccggggat cagagggaac tccagcgccg gcctcatccc 9420

ggccggcatg accttcatcc cttcacgcag gtaccagtcc ggcgggttga tgcccgccgc 9480

gtgcacccgg acgagcacct cgcccgggcc gatctcggga gccggcacct cgtcgtaccg 9540

cagaacttcc ggcccacccg cttcgtggat ctggatcgcc ttcatcgcgc ctgtcgcttt 9600

ctcggggaaa cgttgctcct tcagtcaaag cccgggacgg catggccgtc caagaccggt 9660

tcggcaatct gaccattccg aaacggcatg acgcgtaccc tgcgagggtg aacgatctcg 9720

ggcaggacct ggaactgcgg ctggtgcgct acttcaccgt ggtggcggcg caccagcact 9780

tcggccgggc cgccgccgac ctgcacgtag cccagccggc gctgagccgt cagatccaac 9840

ggctcgagaa atatctcggc acacgactgc tggaccgcac cccccagggc acccggctca 9900

ctccggccgg ccggacgttc ctcccccggg cccaggccct gctgcgggcc gcccgccagg 9960

ccgagctggc cgtgcgtgaa caagcccgga ccgaacgaat cgccatcggc tacgtcgaag 10020

acctggtgat cactgccgcc gtgcgggaac tgcgccgccg ttaccccgac gccgagatcg 10080

ccacccggca tctgcactgc cgcgacgtcg gggcactgtc cgacaggcgc gtcgacgccc 10140

tgatcgcgcg ggccccgctg ccgctcgtcg ccgacgacgt gttcaccacc ccgctgtacg 10200

aggagccccg gatgctcgtg gtcccgcgcg gccatcctct ggccgaccgc gcgtcggtga 10260

ccgcggaaga actggccggc gaagaggcgg cgccgtgtgc gttcgagacc gcaggctggg 10320

cttcctacca gttcctcggg accggcgtgc cgccgatcga gagctacgag gacaagctcg 10380

aactcgtcgc gagcggcagg gccatcgccg tgctaccggt cggcgatcgg cgcagctcac 10440

tgcgtcccga cctcgtcacc gtcccggtcg agggcgctcc ccccagccgg gtcgtcctgg 10500

tcagccgcaa gggcgacccg aatccgatga tcaggaattt ccggctggct gccaaggccg 10560

tcctgaccgc cccggcatcc tgagcgggac caggccgggc gatggccagg ccgacgagcc 10620

gggcggcgcg tggcggttca caacggccac caccgatccg gagcaccttg acgggccgtg 10680

atgctggggc ctctccggga gcgggcgacc tgctcgtgcg gcagtactgc gagcgcagcg 10740

aggactcgcc ctggggggcg ggggagtggc ggaacagcgc gcagtgggaa cgtggcggca 10800

cgaggtcgcc caccgggccg gcgagcgacc gcgtggccca gaggttcggg ctcgccgttg 10860

gcgccgccgt gatgcaggcg ggagattctc gggatgggcc cgcccggtcc ggtagcgcgc 10920

gagtgtcgcg ggacgactga cacggcccca cagaatcggg catcctggga aaatgtctga 10980

cgtgccttgc tccgcttgca gcggatccgg agtcactgag cacaccgagc actctgtcga 11040

agtcgataag gacgggaacc agaagcccgt cgcccgtagc tggaccggct cctgcaatac 11100

ctgccacggc agcggcctca gctgacgaag gggaagcgcc ttgcgatcaa tctatctgtg 11160

cgtcggctgc gcagcgatct acctgccgcc cgagcatccg acggtcggac ggccggtatc 11220

gtcggcgcac tgctcacggc ccgactgccg ggccgcagtg cgccaggccg tcaggatgtc 11280

cggactgccc gaggagaaga tcggagagct ggcccgtcgt gccgccggca tcgacggcga 11340

acctcgccgg ccgagcctcc gcttccgacc cgcacgcggg cgccgagcaa ccgccgctcc 11400

gggcggcggg cgcggcaagc tgcggtagct gtccgatgta gagaagtgac cccgctttcc 11460

tgaacctcat caaccagcgc actgggaagc ggcgatgcgc ttcgtcttcg gtggtctgcg 11520

ctcggtcggc tcgtcgtggg ctttgaggcc tggtgcacgg tggaggtgcg aggtcacctt 11580

ttcgaggatc tcgctgtgcg gaaccttgga ctccggaatc gtctgtatcc gtgcgttcgg 11640

ccgggtgcgg atgcggcgat gaggagaggg gcgagacgat gggtgaaggt gagagggaga 11700

cgtggacgac ggaggagttc gggtcgtccc acgtgggtgc ggtcggggtg ttgctggccg 11760

atggcaccgt cccggatcct gtgctcttcc tcagctcttc cggagcggag gccaggtggc 11820

tatcggagcg gagcgtctac gacggccggc ctcatggcgg accgcgggcg gcagcgttgc 11880

gcgcggtgtg ctcgtgcgga tgggcgggac ccggccgcgc tctcgactgg aacgagagcg 11940

acgaggaaga gggcgaggac ccgagggagg ccgccgaagc cgcatcgccc gcgtccttgc 12000

ggtactcgtt cccccacgcg agcgacaccc gggcagcatg ggccagcgcc cacggcgtgt 12060

acttcccgga cgggctccga agccacgacg actccaacga acaggctgcc ggcacccgcg 12120

cgaacagcgg aagcagcgca ctgggccgat gcctccacac ccgcgctaca tcatccccat 12180

cccgtaccgc ccgaccccct gatggccacg tcgtgcactc cagggatctc gtgtccaccc 12240

ctctgcgctg tcctgctcat tgcagcccgt ccaggggacc ggcaccgatg ccccccgacc 12300

gcaatgccct ctgcgggcaa ccgccgcgct ccccgtaggg gtcaatggcg cgcccttgcc 12360

gccacacccc taatcagaca catgtgtcaa ctgctcatcc gcccactcgg cgaaggtggt 12420

gagcgggatg cccaacttcc gggcgaatgt gggccgggcg ggctggagga ccacgttgtt 12480

ccactcgtgt ccggctcccc acttcggcat gcccgcggca agggcctctt ccacgctcag 12540

ggagggcgcg gtcacgggca cgccccaggc ggcggacaag gtctgcgcga tctgctccat 12600

cgtgcgaagg tcaccagcca gttccagttc cacctggtgg aaccggtcgg ggtctcggag 12660

ggcgtgtgcc gcggccgtgc cgatgtcccg cacggccacc aaggccagtt cggtgtccgg 12720

cttcagtacc gtcagcagtc cgccgcgggg ccccttgggt gccagcaggg gcaggttctc 12780

catgaagaag gcgggcttga tcaccgtcca gcgggcgaaa cccgcaccac gcaccgcctc 12840

catgatcgcc tgcttggtgt ggaagtactc cgccatcgcc gcccagcggc cctcggccca 12900

gccggcgacc cgggtgtgtt caccgactcc actggtcgag gactgtacga actgccgtac 12960

tccccctatc ttcgccgcgt ccaccaggtt ggtggcctgg gcgagttcgc tcgcgaagtc 13020

cacgctggtc tcggtcatgg gcggcatctg caccgagaac accgcgcgga ccccctcgac 13080

cgccgggtcg agggaggccc ggtcggaaag atccgcgcgt accagttccg cgcccagcgc 13140

ctcgatcgcc cgggcggact tcgacgaggg atcgcgtacg agcgcacgta cgggcacctt 13200

ggcggccaaa agcgcgcgag ccgtggcccc gccttgctgg ccggtggcgc cggtgaccag 13260

gacggtatcg ctgctgctgg gcatggtgct gctcctcaag ggccggagag acaaaacggc 13320

ggggcccgcc gtttcgtgtg ccgctaagat atggcggggc ccgccactta gcaacgagga 13380

gaccatgact ggccagcgct cggacgcccg acgcaactat cagcgcatcc tcgccgtcgc 13440

cgaagccgaa gtcgccgcgc acggcgccga ggcatcccag gagcagatcg cccgcatcgc 13500

aggcgtcggt tcggcgaccg tgcgccgtca cttccccacc cgccgggcgc tcctcgaagc 13560

cgtcttccag gagcgcattg cgggcctgtg cgagcgcgcc catcggctga gtgcgtccga 13620

ggacggccgc accgcactgc tggagtggtt ccacgccttc gtccgctacg ccgtctcagc 13680

ccgcggattc gcccatatcc tcagctacga gccgcccacc gaagaaccct ccccgaagag 13740

ctgcgggggc gtaatcgaag cagccgcaac tcccttgctc cagcgggcca tccgcgacaa 13800

ggcggttgca ccgcacgtca cctttcacga cctgctgacc ctcgccgtcg gcatcgccct 13860

ggccaccgag caccacacag accccgacac ccaggccgac cgcctcttcc gcctcgccgt 13920

ggaagggctc agccctcagc cgggcccagc cggggagccc gggaactccg gctcgtcggc 13980

gccggtgaca ggaagtccgc cgaatgagca cgcgtgagaa gtgggatgcc tcctcggtga 14040

caaccgaact gcctcggcac gccctcctcg ccagctcatc cggcgtcgcc gtgagaacgt 14100

cctgagcttc ggcttatcca taacaccgag ggccgtcccc gggtccccag caccctgtga 14160

ggcagctggg gatccggagg aatggacccg cctgacctct ggtggacgcc ttccgagggg 14220

cgacctgagg gactgagggc ggatgacctg tgacagcgaa ccttgatcga gcgccatcga 14280

aggttgacca gtcaccgaat ccgccgtcga ccggatcggc cgacggatcg gatgccgggt 14340

gggtcggtcg gaggaaacgg gacctttgac ccgtgtggag tgttccggag cggatatccg 14400

tatgtcgccg gtcgtccgcg cggagcgacc ggcgggcagc cccggcggag cctcgtcggg 14460

ccttcctcgg caccacctac agcaaaggac gcacaccgtg ggcatcatcg cctggattct 14520

catcggcctc atcgcgggcg ccatcgccaa ggcactcatg cccggcaagg acccgggcgg 14580

ctgcctcgtc acgatgctca tcggcatcgt cggcggtctg ctcggcggct ggctcggcaa 14640

ggtgatcttc ggcgtgcact ccatcaacgg cttcttccac ctctcgacat ggatcgccgc 14700

ggtcgtcggc tcggtgatcg tgctcctgct gtaccgcctc ttcaccggac agcgttcgcg 14760

ctgaccgcga ccggcgatcc acgtctgtcc gtagccggcg tcgagccctg aaccgactgt 14820

gctgcgggcg ggcactgcgc ccctgagctg cggcggtccg tctttgctgt caagaactgc 14880

attcggtcca ccttcgaggc cagtccgctg tcacaggtca gcgggccagc tccgaattgg 14940

tgtcacgatc accgaacgtc acaatctggt tgtcacgggc tctgtcgcgc ccgccgccga 15000

acgggtgaca gtagccgctg ctgctggggg cgagctgtcc ggcctgtcct gtgggatcgg 15060

tgtgggaggc ggcgaatcgg cgttttggga cgccttgtgc gggatgccgc gcgtcgtgca 15120

gcgggcggtg ctggattctc tgctcgtcgg gccgcctggt ggagctggac cggctgcgcc 15180

gcggaccggt acgggtgacg gggcggcgca gagtggtcgc ggtacggggc ggacgacaag 15240

gtgtcgttgc cgcgccggca ctcggactct cgcagcctca caacgctgct ggccgcggcg 15300

gtgtacctca cgtcgcgggc ggtggacgac gcgctggatc tgctgaaggt cctgatcgcg 15360

acgaagcttg cagaccggct gcacatcctg gacggcggtg ctggacgcca cattcgaaaa 15420

gcgcctgctc aatacccgcc cccaacggag acgaggttga acgacaagct cagggcgtct 15480

gagtacttcg gttgagtagt tctgcccatg tgaggggcag ggccgtcgag acaggctgtg 15540

tacacaacgc cgcggtgcgc agagcgctcg gcgaagagac cagtagcggt catctatgag 15600

gggatacttc atgtccaagc gccttgtcgt ctcgtcgctc gccgtggccg cagccgtcgt 15660

tgccggcacc gtggtgttcg tctcctcggc cgacgccgct gtgccggcca agccggagat 15720

ctcaaaggcc accgcccact acaccagtac ggccggtggg agcgcctcgc tcaccttcag 15780

cgccaccgtg gccgacaact ccggaatcaa gagcctgcgg gtgctcgcct ggccggcgag 15840

ttcgggcctt gcgcccacgg cgggcgagat gcgggatgtc gaggaagcca cgtgcaaggc 15900

gacttccgcg acggcctcgg tgtgcaccta caccgtgaag agctcggcca aggaagccgc 15960

cgcgttgccc aagggcgtct ggcacgtatc cgtgctggcc accgccaagg accacgacac 16020

gaccttcgcg ccccaaggcg ccacgttcac cgtcaagcac tgacggctcc gccccgccgg 16080

aatgatgatc gcggcccgcg cgccgggcgg accgtatcgc gacctccatt ggacccgttg 16140

aaccgcacga cgcggtgaac tccgcccacc ctgccccagg gcggacggag ttcacctggg 16200

ggcgacgccg cgcatctgac gcgcgctgcc acgatgccgc accactccgc gcgcgggcgg 16260

atgcaggcga agtgactccc agctcgacgc gctcgccaaa cacggcatct cgcgcgacta 16320

catcttcggc gagaagatca gcacccgggc gcggggcagc ccgaagttcc gggaggaggc 16380

gctgagggcg gcgcgggagg tcaaggcgca cgccccacac tgccgtgtca tcttcacggt 16440

gtacgagcgg aagcggctcg gtcgcaacgc cgccgaactc accgccctcg ccgaccacct 16500

caccgcccac ggcttggtcc tggagatatt cgccgggccc tgtcgaagga ctcccggagc 16560

cgtggaaccc ggcgcccacc cgacgcgaca gcccgacgcg gcagttgggg cgctcccgcg 16620

cggcctgccc ggacaccgaa acgcccggca ccacaagcga aagagcgtcc gtcggcaagc 16680

tgacgggtcc tcatgaagga tttaggccag tgatttggga cacacccgaa cgcgccggcc 16740

ggatctgagg aatcgcctag ggcccgctcc tatcgggaac ttgaagccgc cctgccgagc 16800

caacgcttga ctccggttcc ggcggtgcgg atgacgataa tttccggtga gtctgcccaa 16860

aagggtacat agcgggcgca tagaaaactc ttgcgagtgc tgcgggtggc ttgtagggtc 16920

ctaatgaatc ggctggacaa gggaaggttg atgcgggcgt ccgaaccaaa atagcttcgg 16980

acagcaactg ctgccttctg tcgatggaag tagggggaag ttcgtggaaa tcggctcggg 17040

cgcgcccgaa ttaaccgcgt cgtcggtgta tcagcagcgg cgtgaccaaa tcgccgcaag 17100

cgctgccgcc tatgtgcccg gcgagcccat tccagaggtc gagtacacgg acgccgagca 17160

cgctctgtgg cgcctggttt ccaagcggct cgcggaccgg caccggcaca tggcggcgcc 17220

ggagttcgtc gaggcggcgg agcggctcga ggtgggcggc gacggcgtcc cgcagttgcg 17280

tgaagtgtcc gaccggctcg accagctgac cggattccgc ctacgccccg cgtccggcgt 17340

ggttcccttc gccctgttct gcggctctct ggccgacggg tacttccact ccacccagta 17400

cctccgcgac agcgcgacac ccttctactc gacggaaccg gacatcctgc acgaggtcat 17460

cgggcacggc agcgccctgg ccgacgaccg gttcgccaac ctgtaccgcc tggccggcga 17520

ggccgtgcgc agggtggagt ccgaggacgc cgtccagttc gtcgccaaga ccttctggtt 17580

cacgctcgaa tgcggcctgc tggacgccgc cgacgggccc cgggcctacg gcgccagcgt 17640

cgtctcctcc tacggcgagc tggagcactt ccgttccgcg gagatccggc cgctggacat 17700

cgccgacatg gcccacgtcg actacgacat aacccagtac cagaccacct tttacagcgc 17760

gcgttccctc acgcatctgg aggacgtcgc aggtgaattc tgggcgtcct gcgacgatac 17820

atccattgaa aaactaatgg cggttgatat atgacccggc tcgcagagca atcatccact 17880

gcgcagcaga gcccggaatc agaagtactg gacgtcaccg gaatcggatt cggtgccgcg 17940

aatctcgccc tggcggtggc gctccatgaa tccgaagccg ccgggaaggc ccttttcctg 18000

gagaagcaga aggaattcgg ctggcatcgg gggatgctcc tggggggctc ctcgctccag 18060

gtgtcctttc tcaaggacat cgccacgatg cgcaatccca ccagtgattt cggattcctg 18120

tcctatctcc aggagaagga ccggctggtc gacttcatca accagcacac cctgctgccc 18180

tcccggatcg agtaccacga ctacctccag tgggccgccg accggctgaa ccacctggtc 18240

gagtacggcg tggaggccac cggtgtgcgg ccggtgaccg aagccggtga ggtcgtcgcg 18300

ctcgacgtgc tcgccgggga ccgggtggtc gcccggacca gaaacctcgt cctcgcctcc 18360

ggcctgcgcc cccggctgcc cgagggcgcg gagaccggcg aacgcgtctg gcacagctcc 18420

cagttgctgc accggctgcc cgcgttcgac gaacgcccgc cccgccgggc cgtcgtggtc 18480

ggcgccggcc agagcgcggc cgaggtcgcc gcgcacctca tggaccgcta cccgcaggcc 18540

gaggtgtgcg cggtgttcgc ccgctacggc tacagcgtcg ccgactccag cccgttcgcc 18600

aaccgcgtct tcgacccggc cgccgtggac gacttctact tcgccccgcc cgaggtcaag 18660

caggccatca tgcgctacca cggcggcacc aactacgccg tcgtcgacga ggacgtcctc 18720

cagggcctct accgccgcca gtacgagcag aaggtgtccg gcgccccgcg gctgcgggtg 18780

atgaacgcct cccgcctggt gtccgtcgaa ccgcgccagg aatccgccgc cgtacgcgtg 18840

gagttcctgc ccacgggcga acacaccgac ctggacgccg acctggtcgt gtacgccacc 18900

gggtacgact ccaccgaccc ggccgaactg ctcggcggcg tctccggcgc cctccgccgg 18960

gacgaggcgg gggagttgct gatcggccgc gactaccggc tcggcaccac cggggatttc 19020

cggtgcggca tctacgtcca gggcgccacc gaggcgaccc acggcatcgc ctccaccctg 19080

ctgtccatgg tggcggtccg cgcgggcgag atcgcccggt cgatcaccgg cggccggtgc 19140

gacccggacc gctccaccgg aagcaaggca gcagcgggga acaggggctg aagtgtacga 19200

acgtccgctg taccgggagg attgcgacgg cgtcgtcctg gcgtttctgc gacacaaccc 19260

actggcaatg gtcgtcacct cgcacgacga cgtcccggtg gccacccacg cgccggtgct 19320

gttccggcac ggacccgacg gcgccgacgc cgaggccgtc gccgcgggca ccgtcccgct 19380

cgccggctcc accctgatcg gccacatgaa cgtcgagaac ccgcagtggc gccggatgcg 19440

ctccggcgac cgggcgctca tcgtcttcca gggcccgcac ggctatgtct cgccgacggt 19500

ctacggggtc acgcccgcgg cccccacctg ggacttcatc gccgtccacg tgaacggcac 19560

agtggagccc accgccgacc ccgccgccgt gctggacatc gtctccgaca ccgcccggcg 19620

gctggagtcc ggcttcgggc gcggctggga ccaggagtcc tccctcgact acttccgcca 19680

gatcgcgccc ggcgtgggcg ccttcaccct gcgggtcgat tccgtgcaga cgatgttcaa 19740

gctcagccag gagaagcccg ccccgatgcg gcggcgcgtg gtcgagcagt tcgaagcaag 19800

cgagtccggc acccaccgcg ccctggccag cgtgatgcgc gaccgcggac tcaccgaagc 19860

cgacgaggag cgggagacag ccggatgacg caggggagct cgcgacggcc gggactggac 19920

tcggcactca accggctccg ggagcgcacg gcgaggaccg gcacgacacc cgcgggcatc 19980

agccgggtcc cccgcgaccg gccgctggag ctgtcgttcg cccagcagcg gctgtggttc 20040

ctcgaccggc tgatgccgga cagcgccttc tacaacttcg gcaccgccgt gcgcgtccgc 20100

ggcccgctcg gcgccggcct gctggcccgg gcgctgagct gtgtcgtcgc ccggcacgag 20160

accctgcgca ccgtcttcgc cgaccacgag ggcgtggccg gcgccgtcgt cctgccgccg 20220

gagccggtgc ccgttcccgt gaccgacgcc gtggacgagg ccgacgccga gcggctggcc 20280

ggcgaggagg ccgcgcgccc cttcgacctc accaagggcc ccctgctccg cgcccgcctg 20340

ctgcggctcg ccgacgacga ccacgtcctg ctgctgacgg tgcatcacat cgccaccgac 20400

ggctggtcgc acggcctgct gtgggccgag ctgaccgcgg cctacaccgc cctggccgac 20460

ggccggcagc ccgcgctgcc ggaactgccg gtgcagtacg ccgacttcgc cgcctggcag 20520

cgccgtaccc tgtcgcccgc cgtgctggag cggcggctgg cgtactggca ggaccggctg 20580

gccggcctgc ccccgctgga cctgccgctc gaccggcccc gcccggccgt cgcctccgcc 20640

gagggcggcg tcgtcacctg gcggctgccg gcggacgccg tcgccgccgc ccgggccgtc 20700

gccgcacggc agggcgcgac cctgcacatg accctgctgg ccgcgttcag cgcggtcctc 20760

gggcggcacg cccgcaccga ggacgtcgcc gtcgcccagc cggtcgccgg ccggcccctg 20820

gccgaggtcg agcaactcat cggattcttc gtcaacaccg tcgtcacccg caccgacctc 20880

ggcggcgacc ccaccttcgc ggaactcgtc gaacgcgtcc gcgccgcctc ggtggacgag 20940

atggcgcacc aggacgtgcc gttcgagtac ctggtggagc ggctggtccc cgagcgcgac 21000

ctgtcccgca acccgctcgc ccaggtcgtc ttccagtacg tgccccggcc cgccgcgcgc 21060

cccgcgccgt tccccggcac caccgccgaa cccttcgcgg gcgaccgcgc cttcacccgc 21120

atggacctgg aggtctacct cggcgaggac gccgagggcg gcgtcgaggg actgatcaac 21180

tacagccgcg ccctgttcga ccgggagacc gtcgagcggc tggcccgcca cctgacggcc 21240

ctgctgcgcg ccgcctgcgc cgagcccgac cggccgctgt cccggctgac gatgaccgac 21300

gcgggcgagg acgcggccct ggaccgggcc gcccggggca ccggcgtacc gctgcccgag 21360

gcctcgctgc ccgagctgtt cgccgcccag gccgcccgca ccccggacgc cgtcgccgtg 21420

gccgacggga cggaacacct cacctacgcc cagctggacc gcgccgccaa ccggctcgcc 21480

catgtcctgg ccggccacgg agccggaccc gagagcgtcg tcgcgctggc gacggaacgt 21540

tccgcccacc tggtcgtcgc cgtgctcgcg gtcctcaagg ccggcggcgc ctacctgccg 21600

ctcgacgccc gcaacccggc cgcccggacc cgcgccgtcc tcgccgacac cggtgccgcg 21660

ctgctgctga ccgacggcgg tcccgcaccc gccggcaccg agcacctgcc ggccgtagac 21720

ctgcgcgccg tccccggcac agcccccgac acggctttgc cgaacaccgt cggcccggac 21780

ggcctcgcgt acgtgatgtc cacctccggt tccaccggca cgcccaaggc cgtcgccacg 21840

acccaccgcg ccgtggccgc cctcgccctc caccggcgct ggtccggcgg cgcccacgaa 21900

cgggtcctgc tgcactcccc gcaggcgttc gacgcctcca cctacgaact gtggtccccg 21960

ctgctgtccg gccgccgcgt cgtggtggcc ccgcccggcg ccctcggacc cgccgccctc 22020

gcccgtgtcg tcgccgacca gggcgtcacc gcgctgtggc tgacctccgg cctcttcgac 22080

ctcgtggtcg aggaggacgt cacctgcctg gccggcgtcc gcgaactcgt cgtcggcggc 22140

gacaccgtgt ccccggcgac cgtggcccgc gtgcgcggcg cccacccgga cctgacggtc 22200

gtcaacggct acggccccac cgagaccacc accttcgcca ccctccaccc gatcgcgccg 22260

gccgacccgg cccccggcgg ccgggtgccc atcggcagcc ccctggacaa cacccgcgcc 22320

cacgtcctcg acgaccggct gcggcccgtc ccgttcggcg tgcccggcga actgtacgtc 22380

ggcggcccgc gcctggcccg cggctacgcc ggccggccgg cggccaccgc cgagcgcttc 22440

ctgcccgacc cgtccggacc ggcgggcagc cgcatgtacc gcaccggcga cgtggtgcgc 22500

cgccgccccg acggcgtcct ggagttcctc ggccgcgccg acgaccaggc gaagctgcgc 22560

ggcctccggg tcgagcccgg cgaggtcgag gccgtcctgg ccgcccaccc cgccgtcgcg 22620

cacgccgccg tcgtggtgcg cggtgacggc ccggccggca agcggctcgt cgcccacgtc 22680

gtcccgcgcg ccggccgcac caccgacacc gccgccctgc gcgcccacgc cgcggcggcc 22740

ctgcccgact acctggtgcc ctcggccttc gtcctcgccg acgcgctgcc gctgaccgcc 22800

accggcaagg tcgaccgcgc cgcgctgccc gccccggcgg agacggcgga cgccggcctg 22860

gccgccccgc gcaccgacgc cgagcgggcg ctgtgcgaga tcttcgccga gctgctcgac 22920

gccgacgcgt tcggcgccga ggacgacttc ttcgtacgcg gcggccactc gctgctcgcc 22980

acccgcctcg tcgcccgaat cgcccgcgcc ttcggcaccg aggtcccgct gcgcgaggtg 23040

ttcgagcacc gcaccccgcg agccctggcc tcggtggtcg ccggcgccgc gctcccggcc 23100

gacccggcgc cgccgctggt gcccgcggac cgcgaccggc tgctgccgct gtccttcgcc 23160

cagcagcgga tgtggttcct ggaccagctc gcccccggca gcgcctcgta cacctccggc 23220

ggagccctgc gcgtgcgcgg accgctggac cccgagcggc tggccggcgc cctgtcggcg 23280

gtcgtcgccc ggcacgagac cctgcgcacc accttcacgg tcgccgacgg cgtgcccgcc 23340

gcggtcatcg gcgccgccgc cccggtcgcc ccgcggatcg tcgacgtacc cgacgccgac 23400

gcggcacgcg ccgcggcctc cgccgagctg tccaccgggt tcgacctgac gcggggcccg 23460

ctgctgcgcg ccacgctgct ccggctcgcc cccgacgacc acgtcctcgt cgtcgccgtg 23520

caccacatcg cgaccgacgg ctggtcccag gccctgctgt gggccgagat cgccgccgcg 23580

tacgacggcg ccccgctgcc cgaactgccc gtccagtacg gcgaccacgc cgtatggcag 23640

cggtcctggc tgaccggcga ggtgctggag cgccgggccg gctactggac cggccggctg 23700

gccggcctcg ccccgctgga actgccgctg gacaaggccc ggcccgccgt cgccaccggg 23760

cgggcgggca ccctgccctg gcagctgccc gccgagctga tccgggacgc ccgggccgtc 23820

gccgcccgcg agggagccac gctctacatg gtgctcctgg ccgcgttcac cctcgtcctg 23880

tcccggtacg cccgcaccga ggacatcgcc gtgggctcgc cgacggccgg ccggacccgc 23940

gccgagaccg aggcgctgat cggcttcttc gtcaacgtcg tcgccgtccg caccgacctg 24000

tccggcgacc cgaccttccg ggaactcctg ggccgggtac gggagtcggt ggtcggcgcc 24060

gtcgagcacc aggacgtccc cttcgagcac ctggtggaac ggctgcgccc cgagcgcgac 24120

ctgtcccgca acccgctcgt gcaggtggcg ttccagctgc tcgccgacgc gccccggcgg 24180

ccctggtggc agggcgcccg ggccgagccg ttcgacatcg accacgcgta cacccggatg 24240

gacctggagg tgcacgccgt cgagaccggc gacgaggtcg gcgcgaccgt cctgtacgcg 24300

gccgacctgt tcgacgccga caccgtccgc cagctgatgc accacgtgtc ggtggtcctc 24360

ggcgaggtgc tcgccgaccc cgaccggccg gtctccgcgg cgaccatgct cgacgagacc 24420

gaccggcacc gcaccctggt cgcctggaac gacacggccg caccgctgcc ggacggctgc 24480

gtgccccggc tctacgccga gcaggccgcc cgcacccccg acgccgtcgc cctgatctgc 24540

ggcgacgagc gggtcaccta cgccgaactg gaccggcggg ccaaccgctt cgcccacctc 24600

ctgctggccc acggcgtcgg cgccgacgaa ccggtcggcg tcgccaccgg acgctccacc 24660

ggcatggtgg ccgccgtgct cggcgtgctc aaggcgggcg ccgcctacgt gccgctcgac 24720

ccgcgcaacc ccccgggccg caccgagcgc atcgtggcca cctcggggct gcgcgtcgtc 24780

atcgccgacc gcccggtgcc cggcaccgac ggcatcaccg tcctcgacgt caccgacccg 24840

ggccccgggc ccgacaccga ccccggtatc gacccgcacc ccgacaccac ggcgtacgtc 24900

atctacacct ccgggtccag cggcgagccc aagggcgtgg ccgtgaccca ccgcaacgtc 24960

gtcgtgctgg ccgccgaccg ccgctggagc aacggcaacc acgaacgggt gctgctgcac 25020

tatccgctgg ccaccgacat ctccacctac gagctgtggc cgttcctgct gaccggcaag 25080

cagatcgtcg tcgccaccga cgagcacgtc gaaccgcaca ccttcgaccg gctcatccgc 25140

gagcacggcg tcaccgcgat gtgcctgccc gcacccctgt tcagcctcct ggccgaggag 25200

tgcatggagt gcttcggcgg gctgcgcgag gtgctcaccg gcggcgaggc cgtgtccggc 25260

gagaccgtcg cccaggtgat ggccgcccac ccgcacctga ccgtggccga cgcctacggc 25320

ccgaccgagg cgaccgcgtt caccacgctg ttcccgatgg aacccggctt ccggctcgcc 25380

ggctcccggg tgcccatcgg cgcgccgatc gacaacaccc gggtgtacgt cctcgacgac 25440

accctccgcc cggcgccgct gggcgtggcg ggcgaactgg tcatcggcgg gccccgggtg 25500

gcgcgcggct acctgggccg gcccgacctg accgccgaga agttcgtgcc ggacccgtgg 25560

ggcgggccgg gcgagcggat gtaccgtacg ggcgacgtcg tacggtggct gcccggcggc 25620

gcgctggagt tcctcggccg cgccgacgtc caggtcaaga tccgcggctt ccgggtggag 25680

cccggcgagg tcgaggccgc gctgctgcgg cacccggcgg tctcgcaggt caccgtcgcc 25740

gtgcgcgagg acatcccggg cgacaagcgc ctggtcgcct atgtggtgct ggagccggag 25800

gccggcgcga gcgtgctgcc cgccctgcgc gcccacgccg cgggctccgt acccgactac 25860

atggtgccgt cggcgttcgt cgcgctcgac gcgttcccgc tgtccaccac cggcaagatc 25920

gaccgccggg ccctgcccgc gcccgacacc cgctcggtcg ccgagagcgg gtacgtcccg 25980

cccgcgaccg aggcggagcg ggtgctgtgc gagatcttcg ccgaggtgct ggacgtccac 26040

ccggtgggcg ccgacgacga cttcttcgcc ctgggcgggc actcgctgct ggccacccgc 26100

gccatcgccc ggatccgcgc ccgcttcggg cccgacgtgc cgctccaggc ggtgttcgaa 26160

cggcgctccc cgcgccggct ggccgagacc ctcggcgagc ccggcacggc cacggacgtc 26220

atccggccgg cccgccggga cggcgccgcg cttccgctgt cgtcctccca gcggcgcctg 26280

tggttcctgg accggctcac cccggacagc ggcttctgga acgtggcgat gggcgtacgc 26340

gcccacggcc cgctcgacgt cgacgccctc ggccgggcac tgtccctggt ggtctcccgc 26400

cacgaggcgc tgcgcaccgt cttcgccgcc gacgccggcg agcctatggc cgtcgtacgg 26460

cccgcgaccc cgctccgcct ggaggtcacg gacgtcgccg acgaggccga ggtacgcgcc 26520

ctggccgagg cggacgccgc gcgccccttc gacctggccc ggggaccgct gctgcgcgcc 26580

cgcgtgctgc gcctcgcggc cgaggaccac gccgtcctga tcaccgcgca ccacgcggtc 26640

acggacggct ggtcgcacgc cgtgttctgg ggcgagctgg ccgaggccta ccgcgccgaa 26700

ctgtccggcg accccgccga actgcccgaa ctgcctgtcc agtacggcga cttcgccgtc 26760

tggcagcagg gccggctcac cggcgccgaa ctggagcggt acctcaccta ctggcgggcg 26820

cggctcgccg ggctgcgccc cctggaactt cccctggacc ggccccgccc cgcggtcgcc 26880

ggctcggcgg gcgcctccca gccgtgggaa ctgcccgagg acctggtccg ggccgcgcgc 26940

gcgttcggcg acaccgaggg cgccaccctc tacatgacgc tgctgaccgc cttcaccgtg 27000

gtcctggcac ggttcgccgg caccgaggac gtcgccgtcg gcgcccccgt ggcaggccgt 27060

acccgccccg aggtggagcg gctgatcggc ttcttcgtca acatgctggt gctgcgcacc 27120

gacgtctccg gcgacccgac cttccgcgac ctgctgggcc gggtgcgcga gacggtggtg 27180

ggcgccatgg accaccagga cctgcccttc gagcacctgg tggagaccct ggcacccgag 27240

cgggacctgt cccgcaaccc gctggtccag gtggtcttcc agctgatgcg ggcgcccggg 27300

gacaagggcg accgcctcgg cgccgcccgc ctggaaccgc tgctggacga gcacgccttc 27360

acccgcgtcg acctcgaagt gcacctcacc gaggacggcg accgggtgcg cggcaccgtg 27420

ctgcactcca ccgcgctgtt cgaggcggac acggtccgcc gcctgctgca tcaccacacg 27480

gtgctgctgc gcgccgccct cgccgacccg gacgcgccgc tgtccgcgct ctcgctgctg 27540

gacgacgacg accggcgcct gctgctggag cggtggaacg acaccgccct cccgtaccgg 27600

gacgtcccgc tcgtggagct gttcgccgag caggtcgccc gcacccccgg cgcccgcgcc 27660

gtggagtgcg aggacgacgt cctcacctac gccgcgctgg accacgaggc cgagcggatc 27720

gccgccgggc tgcgggccca gggcgtgggg ccggacgacc tcgtcggcct ctgcctggag 27780

cgcggcaccg tgcagatggc cgctctgatc ggcatcctca aggccggcgc cgcctatgtg 27840

ccgatcgacc cgagccaccc ccgggaccgg atccggctca tcgtcgacga cgcccggatg 27900

accgtcgccg tcaccgaccg ggctcacgcg gacgtcttcg ccgagggcac cgccctcgtc 27960

ctcgtggacg ccccggccgg gcaggagccg cccgcggcaa cggccgccgg acgacccggc 28020

cccgactccc tcgcctacgt cgtctacacc tccggctcca ccggcgtgcc caagggcatc 28080

gccatgcccg cccggtgtgt ggtcaacatg ctcgcctggc agaagaagac ggtaccgggc 28140

ggacccggca cgcgcaccgc ccagttcacc gccctcacct tcgacgtgca tgtgcaggag 28200

gtgctctccg cgctcctgta cggcgagacg ctggtcatcc ccaccgagga gacccgccgc 28260

gacccggccc gcttcgcccg ctggctcgac gaacgggccg tcgagcagat attcgtgccc 28320

aacctgatga tccgcgcgct cgccgaggag gccggcgccg gccgggcccg gctcacctcg 28380

ctgcggcaca tctcccaggc gggcgagccg ctgtcgctgg acaccgtgct gcgcgagttc 28440

tgcgccgccc ggccccgtct gcgtctgcac aaccactacg gctccaccga gatccaggtc 28500

gtcacctcct tcaccctgcc cgctgacgtc gccgactggc cccggaccgc acacctgggc 28560

gagccggtcg acaactcccg cgcctacgtc ctggacgacc ggctgcggcc ggtccccgtg 28620

ggcgtggccg gcgaactctg cttcgccggc cccggactcg cccgcggcta cgtcggcaag 28680

ccggaactga cggcgcagaa gttcgtgccc gacccgttcg gcccgcccgg ctcccgcctc 28740

taccgcaccg gggacctggg ccgctggcgg cccgacggca gcctggaata cctgggccgg 28800

ctcgaccacc aggtcaagat ccggggcttc cgggtggagc tgggcgaggt cgaggccgtc 28860

ctgctgcgcc accccgaggt cacccgcgcc gtcatcgtcg cccgcgagga cgcccccggc 28920

gtcaagcggc tcgtcggcta cgtcgtgccc gtccccggta ccgacggagg gctgccggcc 28980

cggctgcgcg cccacctggc cgacgccgta ccggactaca tggtgccgtc ggcgctcgtc 29040

gcgctggacg cgttcccgct gaccaccacc ggcaagatcg accgggccgc gctgcccgcc 29100

ccggacctgc gcaccacgct cgacaccggc ttcaccgcac cccgtaccgg tgcggagcag 29160

acgctgtgcg aggtgttcgc cgaggtgctg gagaccggcg gggtcggcat cgacgacgac 29220

ttcttcgcgc tcggcgggca ctcgctggtc gcggcccgca cggtcgcccg gatccgcgag 29280

gcgctgggcg ccgaggtgtc cctgcgggag ctgttccagc accgcacccc gcgcgccctc 29340

gccgaggtcg tcgccgtggc cccgcgcacg gccgtaccgc ccctggtgcc ggtgtcccgg 29400

caggagcccc tgccgctgtc gctgggccag ctgcggctgt ggcggctgca cgaggccgac 29460

cccggcgacc cggtctggac gatcccgctg gccgtgcgga tcaccggcga actggacgcg 29520

gacctgctgg gccgcgccct gaccgaggtc gtgcgccggc acgaggcgct gcgcacggtc 29580

ttcgtgcccg gcgacgagcc ggcgtcggtg atcctgcccg ccacggacat cgtgctggac 29640

ccggtggacg tggccgacga gaccgccgcc cgcgccctgg ccgacgaggc agcggcccgg 29700

ccgttcgacc tcgtccgggg accggtgctg cggcccgcac tgctgcggat cgcccccgac 29760

gaccacgtcc tgctgctgac ggtgcaccac atcgccaccg acggctggtc ccagggcgtg 29820

ctctggaccg aactgtccgg cgcctacgcg gcactacgag agaaccgtcc ggccgagctg 29880

cccgaactgc ccgtccagta cggcgacttc gccttctggc agcggtcctg gctgaccgga 29940

gccgcgctcg acgcccagct cggccactgg cgccgccggc tggacggcct gcggcccctc 30000

gccctgccgg gcgtcccggc cgacgcggcc cacgacgcca ccggcgtgct gaccgagtgg 30060

cggctgcccg ccggcctggt cgccaccgcc cgccgggtcg gcgccgagca cgacgccacc 30120

ctctacatga ccctgctggc cgcgttcacg gccacgctcg cccgctgggc gggcacggac 30180

gacctcgccg tcggctcccc ggtggcgggc cgcacccggg ccgaggtcga ggggctgatc 30240

ggcttcttcg ccaacttcgt gcccctgcgc gtcgacctca gcggcgaccc gtccttcgcc 30300

ggcctgctgg agcgggtccg ggacaccgcc ctcgacgcct acgcccacca ggccctgccc 30360

tgggaacgcg tcgtcgaagg cctcggcctg gaccccgaac agccgctggt cgacgtcgtg 30420

ttccagctcg tcaacgtcga actcggcgaa ctcggcctgc ctggcgcccg cgtcgagcag 30480

ttcacgggcc aacaggcgta cgcccgctgg cacctcgagg tgcacctggt cgaggacccc 30540

gacggcggcc tcaccggaca cgtcgtacac cgcgccgccg ccctcggccg acgcgtcgtg 30600

gacggcctgc tggccggcac cgccgcgctg ctacaggccg cactcgccga acccggcctg 30660

cccgtctccg cactgcacgc accggacccg ctggagggga ccacccatga ctgagccgct 30720

gaccgacccg aaggctgctc agccgcccgc gctcgcgcac cggctcgccg gtctgcccga 30780

acccgagcgc cggcgcgtcg tcctggacct ggtccggaca caggtcgcgg aggtgctcga 30840

cctcggcacc gccgccgacg taccgccgga ccgggcgatc cgggaactcg gactgcgctc 30900

gctgaccgcc gtacaactgc tgctgcggct cagccgcggc accggcgtga agctgcccac 30960

caccgccatc tacgaccacc ccaccgcccg cgccctggcg gacgtcctgg tcgacgccgc 31020

ctccggccgg cacgtccggc tcgccgagga cgacgagccc gtacagcgcg cggccgacga 31080

cgacccggtc gccgtggtcg gcatggcctg ccgcttcccg ggcggtgtga ccacccccga 31140

cgagctgtgg cggctcgtgc tggaggagcg cgacgccatc acgccgttcc ccgccgaccg 31200

cggctgggac ctcgccgccc tcgccgaccc ggacggcccc tcggccagcc gcacccggca 31260

cggcggattc ctcgacgacg tcgccctgtt cgacgccggc ttcttcggca tcagcccgcg 31320

cgaggcccag ctgatggacc cccagcagcg gctgctgctg gagaccagct gggaggcgct 31380

ggagcgggcc ggcgtcgcac ccggctcgtg gcggggcgga cgcgtcggcg tgttcgtcgg 31440

cgccaacgcc cagtcctact cctcgctgct cgccggcatc ccggaaggcg gcgacgggca 31500

cgccctgacc ggccgcctgg ccagcgtggt ggccggccgc atctcctacg tcctcggcct 31560

ggaaggcccc gccttcacgg tggacaccgc ctgctcgtcc tccctcgtcg ccctgcacca 31620

ggcggtgcgg tcgctgcgct cgggcgagag caccctcgcg ctggccggcg gtgtgaccgt 31680

catgccgacg ccggagctgt tcgtcgactt caccaagcag ggcggcctgt ccgaggacgg 31740

tcgctgccgc tcgtacgcca aggccgccga cggactcggc tggtccgagg gcgtcggcat 31800

gctcctgctg gagcggctct ccgacgcccg gcgaaacggc cacccggtgc tcgccctgct 31860

gcccggcacc gccgtcaacc aggacggcgc ctccaacggc ctcaccgcgc cgagcggcgc 31920

cgcccagcag cgcgtggtcc ggcaggcact ggccgacgcc ggactgcgtc ccgccgacgt 31980

ggacgccgtg gagggccacg gcaccggcac cgccctgggc gaccccatcg aggcccaggc 32040

actcctctcc tcctacggcc aggaccgcga ccggccgctg tggctgggct cgctgaagtc 32100

caacctcggc cacgcccagg ccgccgccgg cgtcggcgga gtgatcaaga cggtgctcgc 32160

gctccggcac ggactgctcc cgaagacgct gcacgtcgac gagcccaccc cgcacgtgga 32220

ctgggtgtcc ggcgacgtac ggctgctgac cgaggcccgc ccgtggcccc ggggcgagcg 32280

tccccgccgg gccggcgtgt cctcgttcgg cgtcagcggc accaacgccc atgtgatcgt 32340

ggcggaggcg cccgaacccg aggaagcgac ggcgccgccg gccggtgcgc tccccgtgcc 32400

ctggcagctg tccgcgcgca ccgaggccgc gctgctcgaa caggccgccc ggctgcgcga 32460

gttcgtcgcc gccgaccccg gcctcgagcc cgcctccgtc gggttcaccc tggccacccg 32520

ccgcaccgcg ttcgagcacc gcgccgccgt cgtgggccgg gaccgcgccg aactgctcgc 32580

cgcgctcgac gtgctcgccg cgggcggcgc cgacccggcc gtcgtccgcg gcaccgccgg 32640

agccgacggc agcgtcgtct tcgtcttcgc cggccagggc ggacagtggc tcggcatggg 32700

cgtggaactg ctcgacaccc acccggtgtt cgccgcgcgc atggccgagt gcgagcgcgc 32760

cctcgccccc tacctggact ggtcggtcgt ggacgtgctg cgcggagccg aggacgcgcc 32820

gccgctcagc cgcgtcgacg tggtgcagcc ggtgctgttc gcgctcatgg tctcgctggc 32880

cgcggtgtgg cgctcccacg gcgtcgtgcc ggcggccgtg gtcggccact cccagggcga 32940

gatcgccgcc gcctgcgtcg ccggcgccct caccctggac gacgccgcga agaccgtcgc 33000

gctgcgcgcc aaggccgtcg ccgacctgcc cggccgctgc ggcatggcct tcgtcgccgc 33060

ccccgcggcg aacgtcgagc ggatgctgga gcgctggccc ggccggctcg gcatcgccgc 33120

tgccaacagc ccccgctcgc tggtcgtcgc gggcgaccgc gatgccctgg aggagctgct 33180

cgacctctgc gacgacgagg ggctgcgcgc cggccgcgtc gccgccgact acgcctcgca 33240

ctccccgcag gtggaggccg tacggcagcg gctgctcgcc gacctcaagg gcatccgccc 33300

ccgggacggc gacgtgcccc tctactccac cgtcaccgcc gactggatcg acggcagcga 33360

actgggcgcc cgctactggt accgcaacct gcgcgagccc gtgctcttcc agaacgcgat 33420

cagcgatctg ctcgccgccg gccaccacgg cttcgtggag atcagcccgc accccgtgct 33480

cacggtcgcc atgcagcaga ccgccgaggc ggcgggcacc gaactgcggg tcgccgccac 33540

cctgcgccgc gacgagagcg accggctgcg cctgaccacc gcactggccg aggtggccgc 33600

cgacggcgtg ccggtcgact ggcgccccct gttcgacgcc accggcgccc gcccggtcga 33660

actgcccgcc taccccttcc agcgcgagcg ctactggctc accccccagg ccgccgccgg 33720

cgacctgacc gccgtcggcc tcaccgccgg agggcatccg ctgctcgccg ccgaggccga 33780

actccccgac gaggacggcc tgctcctcac cggccggatc agccccgaga cgcacccctg 33840

gctgaccgag cacaccgtgc tgggcaccgc cctgctgccc ggcaccgccg tcctggagat 33900

ggtggcccac gccggccacc ggctcgaccg cgcccagatc gaggagctga ccctggccgc 33960

gcccatcggc gtccccgccg acggcctgac cctgcgcgtc accgtgcacg gcgccgacgg 34020

gtccggccgc cgcacggtcg ccgtccactc ccactccggc gacggctgga cccggcacgc 34080

cacgggcgtc ctggcaccgg ccgagccggc cgagccggcg gagcccgaga ccggcgcgtg 34140

gcccccggcg ggcgctgagc cggtcccggt ggacggcgtg tacgaccgtt tcgccgcccg 34200

cggctacggc tacgggcccg ccttccaggg cctgcgcgcc ctgtggcgcc gggacaccga 34260

ggtctacgcc gaggtcgaac tccccgacac cgtcgacacc gccggccatc tcgtgcaccc 34320

ggcgctgctc gacgccgtca cccaggccgt gcccgccgcc gagcccgagg acgcgccgct 34380

gctgctgccg ttcacctgga ccggcgtgac cgtgcacccc ggcccggccc gggtcctgcg 34440

ggtgcgcgcc gcccgggaga gcgccgacac gctgagcctc accgcgaccg accgcgaggg 34500

ccggcccgtc gtggagctgg ccgcgctgcg gctgcggccc gcgtcggccg ggcagctccg 34560

cgccgtggcc gccgccggca cgcgcgacgc gctgttccgc gtgacctggc agacccccga 34620

cgccgagaca cccgccgccg cgcgctgcgc cgtcctgggc gacggaccgg aaggcctcgc 34680

cgcgcccctg agcaccgccc tcgccgacat cgacgccggc gcggtggacc tcgtcctcgc 34740

tcccgtcagc acaggagccg atgtggtcgc cgccgcccac cgggccaccg cccaggtgct 34800

ggaactgctg cacgagtggc tcgccgacga ccggttcggg caggcccggc tcgcgatcgt 34860

cacccggcac gccgtcgccg cccggcccgg cgaggaaccc gacccggccg ccgccgcggt 34920

ctggggcctg gtgcgctccg cgcagaccga gcaccccgac cggttcctgc tcgtcgacac 34980

cgacggcaca cccgcctccc tggacgctgt gccggccgtc ggcgacgagc cgcagaccgc 35040

gctccgcgac ggcgaacgcc tcgtcgcccg gctcacccgg gccgctgaga ccgcgctgcg 35100

cccgcccgtc ggcgccgacg cctggcgcgt cgacgtggtc cggcccggca gcatcgacgg 35160

cgtcgacgcc gtcgccgctc ccgacgccac cgcccccctg gcacccggcc aggtccgcat 35220

cgccgtccgc gcggcgggcc tgaacttccg cgacgtgctg tgcgcgctcg acatgtaccc 35280

ggacgaggtc gacgcgatcg gctccgaggc cgcgggcacc gtggtcgcgg tcgccccgga 35340

cgtcaccgac ctcgccgtcg gcgaccgagt cctcggcatg gtgccgggcg gcttcggcac 35400

cctcgccgtg gtggaccggc ggctcgtggt gcccgttccc gccggctggt cctgggtccg 35460

cgccgcggcc ctgccgtccg tgttcgccac cgcctggttc gcgctgcgcg atgtggccgg 35520

ggtgcgggcg ggggagcggg tgctggtgca cgcggcggcc ggtggtgtgg gcatggccgc 35580

ggtgcgggtg gcgcggctgc tgggcgccga ggtgtatgcg acggcgagtc ccggcaagca 35640

cgaggtgctg cgggcggccg gtctggacga ggcgcgtgtg gcgtcgtcgc gggatacgga 35700

gttcgcgcag cggttcccgg agatggacgt cgtgctgaac tccctcacgg gtgagttcgt 35760

ggacgcgtcg ctgcgactgc tccgtcccgg cggacggttc gtggaactcg gcaagaccga 35820

ccgccgcgac cccgccggcc tgcccggtgt cgactacctc ccgttcgacc tgctgctgga 35880

cgccggcccc gaccgcgtcc agagcctgct gaccgaggtc gtcgcccacg ccgaggcggg 35940

cgagctgacc gggctgccca cccggacctg gcccctcgcc gacgcgcgca ccgcgttccg 36000

gttcatggcc caggcccggc acaccggcaa gatcgtgctc accgtcgccc cgtacgccga 36060

cggcaccgtc ctgatcaccg gcgccggcgt gctcggcggc atgctcgccc ggcatctggt 36120

gtccgaacac ggcgcccgtg acctggtgct ggccagccgg cgcggcgccg ccgcccccgg 36180

cagcgcggac ctggtcgccg aactggccgc ggcgggcgcc accgtccgct tcgagacctg 36240

cgacgtcacc gaccgcgccg ccctcgacgc gctgctcgcc aagctgaccg ccgaggcccc 36300

gctgaccgcg gtggtgcaca ccgcgggcgc cctcgacgac ggcgtgctga ccgaactcgg 36360

cgccggccgg ctgccgggcg tactgcgccc gaaggccgac gccgcctggc acctgcacga 36420

actcaccgag gacaaggacc tgtccgcctt cgtgctgttc tcctccgcgg cggccaccct 36480

cggcaccccc gcgcaggcca actacgccgc cgccaacgcc ttcctcgacg cgctcgccga 36540

acggcgccgc gccgccggcc tgcccggcac cgccgcggcc tggggcctgt gggccgacgc 36600

caccggcctc acccggcacc tggacgccgc cgacgtcgcc cgcgccggcc gcaaccgcat 36660

cgtgccgatg gccgccgccg aaggactggc cctgttcgac acggccaccg ccaccggcga 36720

cgccgtgacc gtcaccgcgc gcctggacct cgccaccgcc tccgccgcgc ccaccccgcc 36780

gctgctgcgc ggcctggtcg ccaccccggc cggcccggcc gccgcccgcc cggtgcccgg 36840

tgcggcggcc ctggtggcgc ggatcaccgg gctgcccgcg cccgagcgcg cccccgccct 36900

gctcgacgtc gtccgcggcc aggtcgccga cgtcctggga caccggggac gcgacgcggt 36960

ggcacccgac cgcggcttca aggaactggg cttcgactcg ctgaccgccg tggaactgcg 37020

caaccgcctc ggcaccgcca ccggcctgcg actgcccacc accatcgtgt tcgaccaccc 37080

gaacccggcc gcgctcgccg accacctcct cgacgcgctc ctgccgagcg agtccggcga 37140

ggcgagcgcc gaccggatca tcgccgaact ggcccgcgtg gagagcgcgc tcggcgcact 37200

gccggccgac ggcaccgacc gcgcccgggt ggcggcgcac ctgcgggacc tcgcggcccg 37260

ctgggacgcc ggcacgaccg acggcacacc cgagcgggcc gctctggacg gcgtcaccgc 37320

cgacgaactc ttcgacctga tcgaccgggg cgtctcgtga cccgcgccga acgaaccgca 37380

cgggggtgca ccgatggctg acgaggccaa actgctcgac tacctcaagc aggtcacggg 37440

cgatctccag gtcgcccggc gccggctgcg cgaggccgag gcccgcgacc gcgaaccgat 37500

cgcgatcgtg ggcatggcct gccgctaccc cggcggcgtg acctcgcccg aggacctgtg 37560

gcggctggtg accgatggga ccgacgcggt ctcggcgttc cccgccgacc gcggctggga 37620

catggcctcg gtctacgacc cggaccccga ccggcccgga acctcctacg cccgcgaagg 37680

cggattcctc gacggcgccg cggacttcga cgccggcttc ttcggcatca gcccgcgcga 37740

ggcgctggcc atggacccgc agcagcggct ggtgctggag acctcatggg aggccctgga 37800

gcgggctggc atcgacccgg ccggcctgcg ctccaccgcc accggcgtgt tcgtcggctt 37860

cagcagcgag gactactccg acatcaccgg cccggtggcc accgagctgg aggggtacgt 37920

cgtcaccggc acctcgccca gcgtgctctc cggccgggtc gcctacaccc tcggcctgga 37980

gggccccgcc ctctccgtgg acaccgcctg ctcctcgtcc ctggtcgccc tgcacctcgc 38040

ggtccgctcg ctgcgcgccg gggagtgcac gctggcgctc accggcgggg cgaccgtgct 38100

gtccacgccc ggcgtgttca ccgagtacag ccggcagcgg gccctcgcgg ccgacggccg 38160

ctgcaaggcg ttcgccgcgg cggccgacgg gttcggcttc gccgagggcg cgggcatgct 38220

cgtcctggaa cggctctcgg acgcccgccg caacggccac cccgtgctgg ccgtggtccg 38280

cggcaccgcc atcaaccagg acggcgcgag cagcggactc accgccccca acggactcgc 38340

ccagcagcgc gtgatccgcc aggcgctggc cgacgcccgc ctggccgcga gccaggtcga 38400

cgccgtggag gcgcacggta cgggcacccg gctgggcgac ccgatcgagg cgcaggccct 38460

cctcgccacc tacggccagg agcgggacga gccgctgtgg ctcggttcgg tgaagtccaa 38520

catcgggcac acgcaggccg ccgccggtgt ggccggtgtg atcaagatgg tgcaggccat 38580

gcgccacggc acgctgccgc gcacgctcca cgtggacgag gcgtcaccgc acgtcgactg 38640

gaccgccggc gccgtggaac tgctcaccga ggagcgggac tggccgggcg gggagcagcc 38700

gcgccgcgcg ggcgtgtcgt cgttcggcgt gtccggcacc aacgcccacg ccatcctgga 38760

gcaggcaccc gcccggcccg agcccaccga cgaggccacg gaccgcaccc tgccggtcgt 38820

cccgtggacg ctgtcctccc ggacggcggc cggactgcgc gcccaggccg aacgcctcct 38880

cgcccaccgg cccgcgcacg acgccgcccc ccacgacgtg gccctcgccc tcgccaccac 38940

ccgtacggcc ttcgaacacc gcgtggtcgt cctcggcgcg gaccacgaca ccctgctcgc 39000

cggcctgacc gccgtggcgg aaggcgccga gtcggccgac gtggtgcgcg gccgcgccgt 39060

cggcgacggc cgcgccgtct tcgtgttccc gggtcagggg gcgcagtggg tggggatggc 39120

ggtggatctg ctggattcgt cgccggtgtt cgcggggcgg atggctgagt gtgcggtggc 39180

gttggagccg ttcgtgggct ggtcgttgcg tggtgtgctg ggtgatccgg tggcgttgga 39240

gcgggtggat gtcgttcagc cggtgttgtg ggcggtgatg gtgtcgttgg cggaggtgtg 39300

gcgttcgtac ggtgtcgtgc cgtcggctgt ggtcggtcat tcgcaggggg agatcgcggc 39360

tgcttgtgtg gcgggtgtgt tgtcgctggc ggacggcgcc cgggtcgtcg ccctgcgcag 39420

cagggctttg acggcgctgg cgggcagtgg ggggatggtg tcggtcgctg ccgggccgtc 39480

cggtgtcgag gagctgttgg tgggctgggc gggccggttg gcggtggctg cggtcaacgg 39540

tcctgagtcg gtcgtcgtcg cgggtgaggg ggtggcgctg gaggagttcc tggctcattg 39600

cgggggtcgg ggggtgcggg cccggcgtat cgctgtcgac tatgcctcgc attcggtgct 39660

ggtggagccg gtgcgtgagg ctctgctggc tgatctggag ggcgtgcggc cgggtgaggg 39720

cacggttccg ctgttctcca ccgtgacggg ggagtgggcg gacggcacgg cgctggacgc 39780

cggctactgg taccgcaacc tgcgcgaacc cgtccgctac gccgacgccg tcgccgccct 39840

gctcgcccag ggccaccggg gcttcatcga ggtcagtccg caccccgtac tgaccgtcgg 39900

cacccaggag accgtcgagc gcacggagac cggagccgcg gtcgtcggca ccctccagcg 39960

cgaccgggcc ggcctgccga ccctgctgac caacctggcc gaggcgcaca cgcacggcgt 40020

gcgcatcgac tggaccgcct tcttccgggg caccggagcc cacccggccg acctgccgac 40080

gtacgccttc cagcgcgacc ggtactggcc gcccgtggac gcagcggccc gcccgctgcc 40140

cccggccgcc acccccgtcg cgctccccgc gcagcggacc gccgacgagg gcacgccctg 40200

gcgcgacgag ctggccgggc tgacccccgc cgaacgccgg caccgcgtca tggacctggt 40260

acggctgcgg accgccgccg tgctcggcca cgccgacccc gccgcgatcg aaccgcaccg 40320

cggtttcgcc gccctcggct tcgactccct cgcctccctg cggctgcgca ccgcgctgac 40380

cgaggcgacc ggcctgacgc tggccagcag cgtcgtcttc gaccacccga acccggccgc 40440

cctcaccgac catctgctca cccggctcga cggcgcgccc gtcccggccc gccccgccgt 40500

ccgcgccacc ccggccgacg agccgctggc cgtggtcggc atggcctgcc gcttccccgg 40560

cggagtggcc tcgcccgacg acctgtggca gctcctcgcc gaaggccgtg acgtcatcgg 40620

cgacttcccc gccgaccgcg gctgggacct cgacggcctc tacgaccccg acccggaccg 40680

ggccggacac acctacctgc gccagggcgg cttcctgaac gacgccgccg gcttcgacgc 40740

cgacttcttc ggcatcagcc cgcgcgaggc gctggcgatg gacccgcagc agcggctgtt 40800

cctcgaagtc gcctgggagg cgttcgagca cgccggcgtc gatccgcacg gcctgcgcgg 40860

cagcgccacc ggcgtcttcg cgggcgtcac cgaccagcgc tacgactcac ggcacggcgc 40920

ggtcgccggc gtggacgagg ggctgctcgg caccgggaac tacgccagcg tgctctccgg 40980

ccgggtggcc tacaccctcg gcctggaagg cccggccgtc agcgtggaca ccgcctgctc 41040

gtcctcgctc gtcgccctgc acctggccgg ccagtccctg cgcagcggcg aatgctcgct 41100

ggccctcgcc ggcggcgtga tggtcatgtc gaccccgcgt gccttcgtgg agttctcccg 41160

gcagcgcggc ctcgcccccg acggccgctg caaggcgttc gccgccgccg cggacggcat 41220

cggctggtcc gagggcgcgg gcgtggtcgt gctggagcgg ctgtccgacg cccgccgcaa 41280

cggccacccc gtgctggccg tggtccgcgg ctcggcgatc aaccaggacg gcgcctcctc 41340

cgggctcacc gcccccaacg gctccgccca gcagcgggtc atccgctccg cgctggccgc 41400

cgccggcctc accgccgccg acgtcgacgt cgtggaggca cacggcaccg gcaccaccct 41460

cggcgacccc atcgaggcca acgccctgct ggccacctac ggccaggacc gcgcacagcc 41520

gctgcggctg ggctcgctga agtccaacat cggccacacc ggccccgcgg cgggcgtggc 41580

cggcgtgatc aagacggtgc tcgcgctgcg cgccggcgaa gtgccccgca ccctgcacgt 41640

ggacgccccc tcaccgcaca tcgactgggc ctccggctcc gtcgaactcg tcaccggcgc 41700

cgccgtctgg cccccgaccg accggccgcg ccgcgcgggc gtgtcgtcgt tcggcgccag 41760

cggcaccaac gcccacctca tcctggagga ggccccggcg gaaccggccg cgcccgcgcg 41820

cggcgccgcg ccgcgctggg tgccgtggct gctctccgcc cgctcccagg ccgccctcgc 41880

cgagcaggcc acccggctcg ccgcccacct cgacacccac cccggcctgg acccggtgga 41940

cctctcctgg tcccttgccg cgtcgcgggc gttgttgccg catcgtgcgg tggtggtggc 42000

cgctgatgtg gccggtgcgc gggcttctct ggcggcgttg gccgcggggg agccggtgga 42060

gggtgtggtg tcgggtgttg ccgggttgcc gggtgagggc cgggtggtgt tcgtgttccc 42120

gggtcagggg gcgcagtggg tggggatggc ggtggatctg ctggattcgt cgccggtgtt 42180

cgcggggcgg atggctgagt gtgcggtggc gttggagccg ttcgtgggct ggtcgttgcg 42240

tggtgtgctg ggtgatccgg tggcgttgga gcgggtggat gtcgttcagc cggtgttgtg 42300

ggcggtgatg gtgtcgttgg cggaggtgtg gcgttcgtac ggtgtcgtgc cgtcggctgt 42360

ggtcggtcat tcgcaggggg agatcgcggc tgcttgtgtg gcgggtgtgc tgtcgctggc 42420

ggacggcgcc cggatcgtcg ccctgcggtc gcggctcatc gccgagcggc tggccgggcg 42480

cggcggcatg gtctccgtcg ccctgcccga ggccaccgtc acacggcggc tcgcaccgtg 42540

gtccggacgc gtttgcgtcg ccgccctcaa cggcccgtcg tcggtggtcc tcagcggcga 42600

ccccgatgcg ctggacgagg tgatggccgc ctggtcggcc gacggggtac gactgcgccg 42660

catcgcggtc gactacgcct cccactcggc ccatgtcgag tccctcgaag ccgagttgcg 42720

cgccgcgctc gccgaactgc ggcccggtga ggccggcatc gccttccact ccaccctgct 42780

gggcggcccc ctcggcgaga cacggctcga cgccggctac tggtaccgca acctgcggga 42840

gcccgtacgg ttcgagcccg tggtgcgcgg cctgctcgac tccggccacg ccgtgttcgt 42900

ggagatcagc ccgcacccgg tgctgacggc cgccgtgcag gagacggcgg aggccaccga 42960

gcggaccgcc gtcgtcgtcg gcaccctccg ccgcgacgag gacggcccgc gccgcctcct 43020

cacctcgctc gccgaggccg ccgtccacgg cgtgtccgtc gactggacgg tgggctgccc 43080

cgacggacgc cacgtcgacc tgcccaccta cgccttccag cgccgccgct tctggcccag 43140

cggcccggtc gcctccgacg ccgccggcct gggcctgacc ggcgccgggc acccgctgct 43200

cggcgccgtc accccgctcg ccggatccgg cggacaggtg ttcaccgggc gcgtgccggc 43260

cggcaccgag ctgccggccg gcgccgtact cgacctggcc ctgcacgcgg ccgacggcag 43320

gacgctcggc gaactgaccg aggaggcacc gctcgacacc gtcggcgaag cccgccgcct 43380

ccaggtcacc ctgggcgcgg agaacgagga cggcgcccgc ccggtcgccg tccactcccg 43440

ccccgccgac gccgacgacg accagccctg gacccggcac gcgaccggcg tcctcctccc 43500

gcacaccggc accgccccgg ccgccgcgcc aggggacccg gacaccgacg tgaccgtcga 43560

gctgaccgac gtgaccgttg agctgaccga cgagaccgcc ggcggctggg gcgtacaccc 43620

cgccctgctc accgacgccc tcgcggccgt acaccccggc ctggtccccg gcgcctggca 43680

cggcgtcacc ctgcacgccg tgggcgccac ccggctgcgg gtacgcctgc accccgcggg 43740

cgagcacacg gtcaccctgc acgccaccga cgacagcgga gccgccgtgc tcaccgtcga 43800

cgcggtgacc ctgcgcccgc cggccacggc cggccccggc cgcgaccacg agctgtaccg 43860

ggtcgagtgg acgcccgtgc cgctgcccgc ggccgacgtc gacggcatcg ccgtgctcgg 43920

cggccttccg ctgcccggat acccctcctg ccccgacctc gccgccgtcg ccgccctgga 43980

caccgtgccc ggcaccctcg tgctgccgtg tcggcagcag tcggccgacg acaccgccgg 44040

cgcggcccac gccggcgccc ggcgcgtgct gaccgctctt caggagtggc tcgccgacga 44100

gcgactggcc ggcacccgtc tggccgtcct cacccacggc gcggtcccgg tcgagcacga 44160

ggacgtcacc gacctcgccc acgcgcccgt gtggggcctg atccgctccg cccgcgccga 44220

acaccccggc aggctggtgc tggtcgacat cgacggcccc gacgcactgc aacagctgcc 44280

cgctgtcctc gccaccggcg aacccgagat cgccgtgcgc tccggccggg tcctcgcacc 44340

ccggctggtc cgcgccccgc gcaccgacga gcccacgagc caggcggccc tgtggccgtc 44400

cgacggcgtc gtcctcgtca ccggcggcac cggcacgctc ggcgccctct gcgcccgcca 44460

cctggtcacc gagcacggcg tacgccggct ggtcctggcc ggacggcgcg gcgacgcggc 44520

ccccgacgcc gtgaccctcg ccggcgaact gcgcgccctc ggcgccgagg tgaccgtcgc 44580

cgcctgcgac gccgccgacc gcaccgaact ggccgccctg ctcgaccgga tcaccgccga 44640

gcaccccctg accggagtcg tccacgccgc cggcgtcctg gacgacggcg tcatcgcctc 44700

ccagaccccc gagcggctcg ccaaggtgct ccgccccaag gtcgacgccg cctggaacct 44760

gcacgaactc accgccccgc accggccggc cgtcttcgtg ctgttctcct ccacctccgg 44820

cctcttcggg gcgcccggac agggcaacta cgcggcgggc aacgccttcg tggacgcgct 44880

cgcggcacac cgccgcgccc agggcctgcc cgccacctcc caggtgtggg gcctgtgggc 44940

gcacgccagc ggcatgaccg gccacctggg cggcgccgac ctgcggcgcg cggcccgcga 45000

cggcgtggtg ccgctgccga cgcccgacgc gctcgccctg ttcgaccgcg ccaccgccgg 45060

ctccgccccc gtcgtcgtcc ccgcctggct ggacctcacc tcgttcgcca ccggcgtcac 45120

cgccgtaccc gcgctgatgc gccggctggt ccgtggcccc gtccggcgcg ccgccaccgc 45180

gggcgccgga ccggacaccc tggccggaaa gctcaccgga ctcaccgccg cggaacgcga 45240

acggaccctg ctcacgctcg tccgctcgca cgccgccgtg gtcctcggcc acaccgacga 45300

caccgccgtg acccccggcc gggcgttcaa ggaactgggc ttcgactcgc tgaccgccgt 45360

cgaactccgc aaccggctgt ccgccgccac cgggctgcgc ctgcccgcga ccctcgtgtt 45420

cgaccacccc aacccgcgct ccctggccgg ccacctgctg gccgaactcc tcggcgaacg 45480

cgccgaggag acggcgccgg tgcccgccgt cgcccgcccc gccgacgacg atcccatcgc 45540

gatcgtcggc atggcgtgcc ggtaccccgg cggcgtgacc tcgcccgagg acctgtggcg 45600

gctgctcgcc gacgagcgcg acgcgctcac cccgttcccc gacgaccggg gctgggacct 45660

cgcgggcctc ttccacccgg atccggagca cgcgggcacg tcgtacgtgc gggagggcgg 45720

cttcctcgcg gacgtcgccg ggttcgacgc ggacttcttc gggatctcgc cgcgcgaggc 45780

gctggcgatg gacccgcagc agcggctggc cctggagacc gcctgggagg ccgtcgaacg 45840

cgccggcatc gacccgaagt ccctgcgcgg cgcggacgtc ggcgtctacc tcggcaccaa 45900

cggccaggac tatgcccagc tcgtgcgcag gaccgtcgag agcgcggagg gctacgtcgg 45960

catcggcaac tccgccagcg tgctgtcggg ccgcatcgcc tacgtcctcg gcctggaagg 46020

ccctgcggtc accgtggaca ccgcgtgctc ggcgtcgctg gtcgccctgc actgggcgat 46080

ccaggcgctg cgcagcggcg agtgctcgat ggcgctggcc ggcggtgtca ccgtcatgtc 46140

cgcccccgac gtcttcgtcg acttctcccg ccagcgcggc ctggccgtgg acggccgctg 46200

caagtccttc gccgccgcgg ccgacggcac cggctggtcc gagggcgccg gcatgctcct 46260

cgtggaacgg ctctccgacg cccgccgcca cggccaccag gtactggccg tcgtacgcgg 46320

caccgcgatc aaccaggacg gcgcgtcgaa cggtctgacg gccccgaacg gtccttcgca 46380

gcagcgggtg atccgtcagg cgctctccgg tgccgggctc acgcccgcgg acgtggacgc 46440

ggtggaggcg cacggtacgg gcacgcggct gggcgacccg atcgaggcgc aggcgctgct 46500

cgccacctac gggcaggagc gggacgagcc gctgtggctc ggttcggtga agtccaacat 46560

cgggcacacg caggccgccg ccggtgtggc cggtgtgatc aagatggtgc aggccatgcg 46620

gcacggcacg ctgccgcgca cgctccacgt ggacgagccg tcacctgagg tcgactgggc 46680

ctcgggcgcg gtggagctgc tgaccgaggc ccgggagtgg gcgcgtgccg gtcggccgcg 46740

ccgtgcgggt gtgtcgtcgt tcggtgtgtc cggcacgaac gcgcatgtgg tgctggaaga 46800

ggctcccgag gagaccgggc gcaccgcgcc ggctgcgctg cccgccgtgc cctggctgtt 46860

gtcggcccgc tccgagaccg cgctgcgcgg acaggtcgag cggctgcgtg cgtacgtcac 46920

ggagcacccg gaggcgcggc cggccgacat cggcctcagc ctcctcaccg cacgctcccg 46980

cttcgagcac agcgccgtcg tcgtcggcac cgaccacgac gcactcctcg ccgcgctggc 47040

ggacccggag ccgctgcacg cacgtgacgg cctgaccgcg ttcgtgttcg cggggcaggg 47100

tgcgcagcgg gtggggatgg gggcggaact ggctgccgcg tatccggtgt tcgctcaggt 47160

gttcgcgcag gtgtgtgcgg cgttcgacgg tgtgctggag cggccgctgg gtgaggtggt 47220

cgccgagggt ggtccggagc tggatcggac ggtttatgcg caggcggggt tgttcgcttt 47280

cgaggtggcg ctgttccggc tgctggagtc gtggggtgtc gcgccggatg tggtgctcgg 47340

gcattccgtg ggtgagttga cggctgcctg tgtggcgggt gtgtggtcgc tcgaggacgc 47400

ggtgcgggtg gtggctgcgc gtgggcggtt gatgcaggcg ttgccggagg gcggtgcgat 47460

ggtcgctctc gaggtgtcgg cgggtgagct ggagttgccg gtgggtgtgg agctggcggc 47520

ggtcaatggc ccgacgtcgg tggtgctgtc cggtgaggag gaggccgtcc tggctgaggc 47580

cgcccgctgg ccggaccgtc gtgccaagcg tctgcgggtc agtcacgcct tccactcgca 47640

tcggatggat ccgatgctgg aggacttccg gcgggtactg gagtccgtga ccttccaggc 47700

tccggaggtt gccttcgtgt cgacggtgac cggtgctgcg gtgacggacg agctgtgcga 47760

cccggggtat tgggtgcgca acgtccggga gaccgtccgc ttcgccgacg ccgtcgccgc 47820

cgtgcgcgcg accggtgccg acgccctggt cgagatcggt cccgatgccg ccctcgctcc 47880

gctcgccgag ggcggtgtgc cgctgctgcg caggggccgg ccggaggccg tcgcgctggt 47940

cgaggggctc gcccggtccc agatggccgt cgactgggag aagttcttcg gcgccggggt 48000

gagccccgtc gacctgccca cgtatccgtt ccagcacagc cggttctggc cggaggtccc 48060

ggtgcgggcc gaggccgatc cggccgcgcg gtggcgttac cgggtgcggt gggagccgct 48120

ccgtcccgcc gtggacgcgg tgccggccgg gcgctggctg gtggccgtgc ccgccgcggg 48180

cgcggatgcg gctctggtgc gggagtgcct gaaggggctc gccggccgag gagtggaggt 48240

ggccgagctc gcggtggcgg gtgcgcccga ccgtgcgcgg ctcgccgaag cggtgtcggc 48300

cacgggcccg gtggacggcg tgctgtccct gctcgccgcc ggtcctgacg cggcggccgg 48360

caccctcgtc ctcgcccagg ccctcggcga cgccgggacc gacgcgccgc tgtggtgcgt 48420

gaccggcgga gccgtcgcgg ccgccgacgg agaacccgcc gacccggagc aggcccaggt 48480

ctggggcctc ggccgggtgc tgtgcctgga ggcgccgcac cgctggggcg gcctcgtcga 48540

cctgcccgcc gagcccgacg agcaggcggt cgtacagctg tgcgccgtcc tcgccggaca 48600

cgacgacgag gaccaggtcg ccgtgcgggc ggccggggtc ttcgggcggc ggctggtccg 48660

cccggccacg ggcgccgcgg cggagggctg gcggccgtcc ggcaccgtgc tgatcaccgg 48720

cggcacgggc gcgctgggcg ggcacaccgc ccgctgggca gcccgctcgg gcgccggccg 48780

ggtggtgctg gtgagccggc gcggacccga cgcggacggc gcggcggaac tcgtggcgga 48840

actcggcgaa ctgggctgcc aggccgtcgc ggaggcgtgc gacgtggccg accgggccgc 48900

gctcggcgcg ctgctcgaca agatcgccgc cgacggcccg ccgctgaccg cagtggtgca 48960

caccgcaggc gtcctggacg acggggtgca cggctcgctg acccccgaac ggctggccac 49020

ggtgctgcgc gccaaggccg acggcgccac cgccctgcat gaactcaccg cccacctgcc 49080

cctggaggcg ttcgtcctgt tcgccgcgct cggcggggtg gtcggcggcg cgggccaggc 49140

caactacgcc gccgccaacg cccacctcga cgcgctcgcc gcgagccgcc gggccgccgg 49200

gctgcccgcc accgcggtgg cgtggggcgc ctgggcgggc ggcgggatgg ccgacgcgga 49260

cgaggtacgc cggcgcctgg accgcgacgg gctgctgccg atggacccgc ggcgggcgct 49320

ggacgctctc ggccgggaga tcgcggccgg cgacccggcc gtggtgctcg ccgacgtcga 49380

ctggacccgg ctcgcgccca acctgcacgc cgtccggccc agcccgctga tctcgaccgt 49440

gcccgaggca cgccgggccg tcgcgcccga acccggcacg gccggcggcg agtccgatcc 49500

gcggcagcgc ctggcggccc tgcccgacgg cgaacgcgcg cgcgtgctgc tcgacctcgt 49560

ccgcgacgcc atcgccgccg tgctcgggta cggcggtccc ggcgcggtcg acatcacgcg 49620

gggcctcgtc gacctgggct tcgactcgct gaccgccgtc gaactgcgca accggctcgc 49680

ccgggccacc ggactcgccc tgccgctcac gctggtcttc gaccaccccg acggggaggc 49740

gctcgccgcc cacctcgccg ccgcactcgc gcccgccgcg gaccggccgc agaccaccga 49800

cctcgaccgg ttcgcccggc tcgacccggc cacggccgac gagccgaccc gggtccgggt 49860

cgccgcggag ctgcgccggc tgctcgacgc gtggacgccg ccaccggccg cgcccgccgg 49920

gtccgccggg tccgccgcgg accccagccg gctgaccacg gcgtccgccg acgagatctt 49980

cgacttcatt cggaacgaac tcgggaagtc ctgaacgtgg agaacgagaa caagctcctc 50040

gaccacctgc ggtgggtcac cggagaactg gcccaggcac ggcagacgct gcgcgagacg 50100

gccgaacgtg ccaccgagcc gatcgcgatc gtcggcatgg cctgccggtt cccgggcggc 50160

gtgagcacgc ccgaggacct gtggcggctg ctcgccgacg agcgtgacgc ggtcggcgag 50220

ttccccgccg accgcggctg ggacctggcc tcgctgcacc acccggaccc cgagcacgcg 50280

ggcacgtcgt acgtgcggga gggcggcttc ctcgcggacg tcgccgggtt cgacgcggac 50340

ttcttcggga tctcgccgcg cgaggcgctg gcgatggacc cgcagcagcg gctcgccctg 50400

gagaccgcct gggaggccgt cgaacgcgcc ggccttgacc cgaagtccct gcggggcgcg 50460

gacgtcggcg tgtacctggg caccaacgga caggactaca tctccgccgc gcgcccgctg 50520

ctgcaccagg tggagggcca cggcggcacc ggcatcgccg gaagcgtgct gtccggacgc 50580

gtcgcctaca ccctcggcct gcgcggcccc gcggccaccg tcgacacggc gtgctccgcc 50640

tccctcgtcg ccctgcactg ggcgatgcgg gcgctgcgcg gcggcgagtg cgcgatggca 50700

ctcgcgggcg gtgtgacggt catgtcgtcc ccggcgacct tcgtggagtt ctcccggcag 50760

cgcggcctcg cctccgacgg ccgctgcaag ccgttcgccg ccgcggcgga cggcaccggc 50820

tggggcgagg gcgccggaat gctgctgctg gagcggctct ccgacgcccg gcgcctcggc 50880

cacccggtgc tcgcggtgct gcgcggctcc gccgtcaacc aggacggcgc cagcagcgcc 50940

ctcaccgcgc ccaacggccc ggcgcaggtc cgcgtcatcc aggcggcgct cgccgacgcc 51000

cggctcgcgc ccgccgacgt cgacctgctg gaggcgcacg gcaccggcac cgtcctcggc 51060

gacccgatcg aggcccaggc cctgctcacc gcctacggcc agaaccgcac cgcccccgcc 51120

tggctcggct cggtcaagtc caacgtcgga cacacccagg ccgcggccgg ggtcgccgga 51180

gtgctcaaga ccgtgctcgc cctgcgtcac ggggtgctgc cccggaccct gcacgtggac 51240

gcgcccaccc cgaaggtcga ctggtcggcg ggcgcggtac ggctgctcac cgaggcccgg 51300

ccgtggcccg ccggcgagcg gccgcggcgc gccggtgtct ccgcgttcgg tgtcagcggc 51360

accaacgcgc acgtcatcgt ggagcaggca cccgagaccg ccgccgaggc gtccggcgcg 51420

gccggcgggc cggtgccgtg ggtgctgtcc ggccgtaccg agagcgcgct gcgggcccag 51480

gccgccgcgc tcgccgccca tctggcggag cggcccggcg accggcccgg ggacgtcgcc 51540

ctgtccctgg ccaccacccg cccggcgttc gaacaccgcg ccgtcgtggt cgggacggac 51600

gtcgacgacc tgctgcgcgg cgtggccgcc gtcgccgcgg gagagcccac gcccggggtg 51660

gtccgcggca ccgccggcca cctcggccgg atcgcgttcg tgttccccgg gcagggctcc 51720

cagtggaccg gcatggcccg cgaactcgcc gacgcctcaa cggagttcgc cgcccgcctg 51780

gacgagtgcg ccgccgccct cgccccgcac gtcgactggt cgctgcgcga cgtcctggcc 51840

gacccggccg ccctggagcg cgtcgatgtc gtccagcccg ccctgtgggc cgtgatggtc 51900

tccctggccg ccctctggca ggcgcacgga gtggagccgg ccgccgtcgt cggccactca 51960

cagggcgaga tcgccgccgc gtgcgtcgcc ggcgccctct ccctggagga cgccgccctg 52020

ctggtcaccg cgcgcgcccg ggcgctgcgc gccctgatcg gccggggcgg catggtgtcc 52080

ctgccgctgc cggaggccgc cgcccgcgaa gtcatcgcag cgtggggcga ccggctgtcg 52140

gtggccgccg tcaacggccc cgccgcgaca gtcgtgtccg gcgacgccgc cggcctggac 52200

gaactcctcg cccaggccga gcgggacggc ctgcgggcca agcgcctccc cgtcgactac 52260

gcctcgcact ccgcgcacgt cgaagccgta cgcgccgagg tactggccgc gaccgccgcc 52320

gtcaccccgc gcgccaccgg caccgccttc gtgtcctcgg tgaccggcgg attcctggac 52380

accaccggtc tggacgccgc ctactggtac cgcaacctgc gcgagccggt ccgtttcgac 52440

cgggcggggc gggccctgct ggacgccggg ttcacgacgt tcatcgaggt cagcgcgcac 52500

cccgtgctca ccgccgcact ccaggagagc gacccggccg tcctcgccgt cggctcgctg 52560

cgccgcgacg acggcggccc cgcccgcttc ctcgcctccc tcgccgaggc cgcggtccgc 52620

ggcgtaccgg tcgactggcg accggccctg accgcggccc gcaccgtgcc tcttcccacc 52680

tacgccttcc agcgcgaacg cctctggctg gaggaggacg ccgggccgat caccccggaa 52740

cgggcccgcg aggacagcgc gttctgggcc gcggtccacg gctccgccga cgacctggcc 52800

gcggtgctcg gcgcggaccc cgccgcccgg gagccgatcg ccgccgttca cccgctgctc 52860

gccgcctggc acgagcggcg cgccgacgag tccgccgtgg actcctggcg ctaccgcgtc 52920

gactggaccc cgctcaccgc gtcgccctcc gccggcgcgc ccaccggccg ctggctggcg 52980

gtcgcggccg acgacccctg gtccgacgcc gtcgtcacgg cactggcgga ccgcatggac 53040

ctcacccgcg tcccggccta cgaccccggc accgtcgcgg aactcaccgc cgggaccgcg 53100

ggcgtcgtcg ccgtgctccg ccccgacgac cccgacccgc tccccggcct cctcgccctc 53160

ctccgggccc acgaccgcgc cgacggcggc ctgccgctgt ggtgcgtcac ccgcggcgcc 53220

gtggcgaccg gcccggcgga caccccggcg gacccggcca ccgcgcagat ctggggcttc 53280

ggccgcgtcg ccgcgctgga acgaccgcag agctggggcg ggctgatcga cctgccggcc 53340

ggcgccaccg tgaccgggga cgcgctggcg ggcacggccc gggccgacgg cccaccggac 53400

gcccgcatcg ccgacgacgt cctcggcgcc cgtctcgccg accggctcgt cgccgcgctg 53460

gcgggtccgg aggaccaggt ggcggtccgc gcctccggcg ccttcggccg ccggctgcgc 53520

cgcgcgcccg cagacgggac cacccccgac tggaggccca ccggaaccat cctcgtcacc 53580

ggcggcaccg gtggtctcgg cgggcacgtg gcccgctggc tcgcccgtgc cggggcggag 53640

cacctcgtgc tcaccgggcg acgcggcccc gaggcgcccg gcgcggcgga actggccgcc 53700

gaactcaccg cgctcggcgc cgaggtcgac atcgtcgcct gcgacgccgc cgaccgtgac 53760

gcactggccc gggtgctggc cgagcacccc gtcgacgccg tgttccacct cgccggcatc 53820

gagcgctacc ggccgctgga cgagctgacc ccggccgatc tcaccgaggt cgccgccgcc 53880

aaggtcaccg gcgccctcct gctggacgaa ctgacccgcg accggcggct gtccgcgttc 53940

gtgctgttca cctcgggcgc cggagtgtgg ggcagcagcg gccaggccgc ctacgccgcc 54000

gccaacaccc gcctcgacgc gctcgccgcc cggcgccgcg ccgagggcct gcccgccacc 54060

gccgtcgcct ggggacactg ggacggcgcc ggcatgtccg acggcccggc ccgcgcccag 54120

atgctccgct ggggcctgcc cgccatggcg ccccaccgcg ccgtcgccgc cctcgccggc 54180

gccctcgccc gggacgaggc ctcgctggtc gtcgccgaca tcgactggcc ggtgttcgcg 54240

ccgacgttca ccctggcccg gcgcagcccg ctcctcgccg gcctgcccga ggccaccgac 54300

ccggccgaca ccgccccgcc gcccgccgcc ccggccggcc ggctggaccg ggccgcgctg 54360

ctggagctgg tgctcgccga gaccggcgcc gtcctcggcc acgcctccgg caccggactg 54420

cccgccgccc gcgccttcca ggaactgggc ttcgactcgc tcaccgccgt ggaactgcgc 54480

aaccggctgc gcgacgcgac cggactgccc ctgcccgcca ccctcgtctt cgaccatccc 54540

accccgcagg cgctcgccga gcgcctggcc ggggaactca ccggcgaaca gcccgccgac 54600

gttccggaca ccacgccggc gccggccgcc gccgccgacg acgacccggt cgtcatcgta 54660

ggcatgggct gccgcttccc cggcggcgcc gactcgcccg aacggctgtg ggacctcgtc 54720

gccgcgggcc gggacgcgat gaccggcttt cccgccgacc gcggctggga cgtcaccgac 54780

accggctaca ccaaggccgg cggattcctc gacgccgccg gtgagttcga cgccgggttc 54840

ttcggcatca gcccccgcga ggccaccagc atggacccgc agcagcggct gctgctggag 54900

acggcgtggg agacggtcga gcgcgccggc atcgacccgg cctcgctgcg cggcagccgg 54960

accggcgtgt tcgtcggcta cggcggccag gactacctca ccagcctgta cggcaccccc 55020

gaggaactac agggccacct cctcaccggc acctccggca gcgtggtctc cggccggctc 55080

gcctacgtcc tcggcctgga gggccccgcg gtcaccatcg acaccgcgtg ctcctcgtcc 55140

ctggtcgccc tgcactgggc gatccgcgcg ctgcgctccg gcgagtgcga cctggccctg 55200

gcaggcggcg tgaccgtgat ggccaccccg ggcgtcttcg tcgagttcgg ccgacagggc 55260

ggcctggccg gcgacggccg ctgcaaggcg ttcgccgacg ccgccgacgg caccggctgg 55320

ggagagggcg ccggcctgct cctggtggag cggctgtccg acgcccgccg aaacggccac 55380

cgcgtcctcg ccgtcgtccg cggctccgcc gtgaactccg acggcgcctc caacggcctc 55440

accgcgccca acggcccctc ccagcagcgg gtcatccggg ccgcgctggc cgacggcggc 55500

ctcaccccgg ccgacatcga cgccgtggag gcacacggca ccggcaccgc cctcggcgac 55560

cccatcgagg cccaggccct acaggtggtc tacggccagg accgcgagcg cccgctgtgg 55620

atcggctcgg tcaagtccaa cctgggccac acccaggccg cctccggcgc cgccggcctc 55680

atcaagacgg tcctggcact gcggcacggc gtgctgccgg ccaccctgca cgtcgaccgg 55740

ccctccgcac aggtcgactg gaccagggga gcggtgtccg tcctcaccga gagcactccg 55800

tggcccggga ccgacgtgcc gcgccgcgcc gccgtgtcct ccttcggggt cagcggcacc 55860

aacgcccatg tcgtcctcga acaggccccg cccgccgggg aagccggacc ggccggggac 55920

ggcggacccg tcccgtggct ggtctccggc cggacgcccg aggccgtacg cgcccaggtg 55980

gagcggctgc gcgccagcct cgccggacac cccgacccgg tcgccgtcgc ccgcgccctc 56040

gccaccacgc gcaccgcgtt cgagtaccgt gtcgccgccg cgggcggcga caccgaggcg 56100

ctgctcgacg ccctcgccga ggcccgaccc gtcaccgccc gccagggccg taccgcgttc 56160

ctgtgcaccg gccagggcgc ccagcgcgcc ggcatgggtg ccggcctgta cgccgcccac 56220

cccgtctacg ccgacgcctt cgacgccgtc tgcgccgagt tcgaccgcct gctggaccgc 56280

ccgctgcgcg acctggtgct gtccggcccc gccgacgtac tcgaccgcac cgcctacgcg 56340

cagcccgcgc tgttcgccgt cgaggccgcc ctcgccgcac tgctgcgcca ctggggcgtg 56400

acccccgacc tgctggccgg ccactcgctc ggcgagatca ccgccgcgca cctggccgga 56460

gtcctctccc tgccggacgc cgcggccctg gtcgccgccc gcggccggct gatggacgcg 56520

ctgccggccg gcggcgcgat ggtcgccgtg gaggccgacg aggaccgtgt acagcccctg 56580

ctgggcgacg acgtctgcct cgccgccgtg aacggcccgc gcgccctggt gctgtccggc 56640

cgcgaggagg ccgtggacgg ggtggccgcg cggctcgccg ccgaggggtg ccggacccgc 56700

cgcctgcggg tgtcccacgc gttccactcc gcactgatgg agccgatgct ggacgagttc 56760

cgcgccaccg tggccgccct cgacctgcgg gcgccctccg tccccgtggt gtccgcgctg 56820

accggccgcc cgctgacggc cgacgaggcc cgctcacccg agcactgggt acggcacgtc 56880

cgcgaggccg tccgcttcca cgacgccgtg cggggacttg ccgccgaggg cgccgtacgg 56940

tacctggaac tcggcccgga cggtgtgctc accgccctcg cccagagcag cctgccgccc 57000

gccgacaccg acgccgacgg gcgcgacccg ctcgccgtgc ccctgctgcg ggccggccgg 57060

ccggaaccgg agacgctcac cgacgcgctg gcccgcgccg ccgccgacgg cctgaccgtc 57120

gactgggccg gctacttcac gggccgcggc ggcgcccccg tcgagctgcc cacctacgcc 57180

ttccagcgcg agcactactg gctgcccgtc gactccggcg ccggcacggc cccggccggg 57240

caccccctgc tgtccgccgc ggtcgacctg ccggacggcg gcctcgtact caccgggcgg 57300

ctctcgcccg ccgcgcgccc ctggctcgcc cagcacaccg tgcgcggcag cgccctgctg 57360

cccggcaccg ccctgctgga cctggccctc gccgcggccg gccaggcggc cgcgcccggc 57420

gtcgccgaac tgatcctcga agcccccctc gtgctgcccg ccgagggcgc cgtggaggtg 57480

cgcgtcaccg tcggcgccgc cgggaccgac ggccgccgcg cgatcgccct gcacacccgc 57540

gccggcgacg gcgactggac ccggcacgcc accggagccc tgggcgaggt gcccggcgag 57600

cccacggccg ccggcgcctg gccgcccccg gatgcccgcc ccgccgacct cgccgccctg 57660

tacggcgggc tggccgacgc cggcttcggc tacgggcccg cctaccaggg cctccgtgcc 57720

gcctggcggc gcggcgaagg cccggcggcc gaggtgttcg ccgaggccga actgcccgcg 57780

gccgtccccg acgccgaccg ctgccccgta cacccggcgc tgctggacgc cgtcctgcac 57840

gccatcggcg tgggcgggct gatcaccgac ccggcgcacg gcggactccc gttcgcctgg 57900

accggggtac gggtcttcgc ccccggcgcc cgggcggtcc gcgcccggct ctcccgggcc 57960

ggcgccgaag gcgccctcgc cgtcgacctg ttcgacgccg acgggctgcc agtggccgcc 58020

atcggctccc tgcggctgcg cccgcccgcc gctcccgcgg tgcccgacgc cctcttcgag 58080

accgcctgga cgcctgtcga gcagggcacg gccccggccc gccgcctcgc gctgctcggc 58140

gccgacaccg ccctcgcggc cggcctcacc gcggccggcg ccgccctcgc cgacgccacg 58200

gaccgctccg ccgaggtgct cgtcctgccg atcgtcaccg accccggcgc ggcccccgtc 58260

accgagaccc accgggcgac cgccgccgtc ctgacggccc tccgtgacgt cctggccgac 58320

gaggagagca ccgcccgcct cgccgtggtc acccgcggcg ccctcgcgct gtccgccgag 58380

gagtccccgg acccggccgc ccgcgccgtc tggggtctgg tgcggtccgc ccagaccgag 58440

caccccgacc ggatcgtcct cgccgacctg gacgccgccg acgcctcggc ccgcgccctg 58500

cccgccgcgc tgacctgcgg ggaaccgcag ctcgccgtgc ggtccggcgc ggtcagcgca 58560

ccccggctca cccgcgccgg cgccgacgcg ctggtcctgc ccgacggcgg ctggcgcctg 58620

cggcccggcg ccaccggcac cgtcgacggc atgacggccg tgccgcaccc cgacgcgccg 58680

ctcgccgacg gcgaggtacg ggtcgccgtc cgcgcggtcg gcgtcacctt ccgggacgtc 58740

ctcagcgtgc tcggcctcta ccccggggca ccccagccgc tcggcatcga ggcggcgggc 58800

gtggtgaccg ggaccggccc cggcgtgagc gacctcgccc ccggcgaccg ggtgttcgga 58860

ctgctgcccg gctccatggg ctcctccgcc gtcgccgacc ggcgcgtgct cgcgcccgtc 58920

cccgacggct ggggcttcac ccgggccgcc tcggtgccct ccgcgttcct caccgcctgg 58980

ttcgcgctgc gcgatgtggc cggggtgcgg gcgggggagc gggtgctggt gcacgcggcg 59040

gccggtggtg tgggcatggc cgcggtgcgg gtggcgcggc tgctgggcgc cgaggtgtat 59100

gcgacggcga gtcccggcaa gcatggggtg ctgcgggcgg ccggtctgga cgaggcgcgt 59160

gtggcgtcgt cgcgggacac ggagttcgcg cagcggttcc cggagatgga cgtcgtgctg 59220

aactccctca cgggtgagtt cgtggacgcg tcgctgcgac tgctccgtcc cggcggacgg 59280

ttcgtggaac tcggcaagac cgacctgcgc accgacaccg ccggcatcac ctaccgggcc 59340

gtcgacctcg cggacgccgg ccccgaccgc atccaggaga tgctcaccga actcctggac 59400

cgcctcgcgg ccggcgacct cgcccacctg cccgtccgca gcatgcccat gggccgcgcc 59460

cgcgaggcgt tccgcttcat ggcccaggcc cggcacaccg ggaagctcgt cctcaccacc 59520

gccccgtacg gcgacggcac cgtcctcgtc accggcggca ccggcgccct cggcggcctc 59580

gtggcccggc acctcgtcac cgaacacggc atccgcgacc tggtgctcgt cggacgacag 59640

ggcgccgagc cccccgtcac cgccgaactg cgcgccgccg gcgcccgggt ccgcgtggcg 59700

gcctgcgacg tgtccgaccg ggccgcgctc gccgcgctgc tcgcggacat cgagccgccc 59760

ctgaccgcgg tggtgcacgc ggccggcgtc ctcgacgacg gcacgctcac ctcgctgacc 59820

cccgaacggc tcgccgccgt actgcgcccg aaggccgacg ccgcctggca cctgcacgaa 59880

ctcaccgagg acagggacct gtccgccttc gtgctgttct cctcggcggc cggcacgttc 59940

ggcgcccccg gccagggcaa ctacgccgcc gccaacgccg ccctggacgc gctcgccgag 60000

caccgccgct cccgcggcct gcccgccgtc tccctcgcct gggggccgtg ggccgccgag 60060

agcgccatga ccggcggcct cagcagcggc gaccgcgcca ggatgacccg ggccggcgtc 60120

cggcccctgg ccgccaccga ggcactcgcc gtgctcgacg ccgcctgccg caccggagcc 60180

ggcgccctcg ccgcgctccg tctcgacacc gcggcgctca ccgcccgcac cggcgccccg 60240

cacccgctgc tgcgcgacct ggtccgccgt ccggccggcc ccgcccgcga cgacgccgac 60300

acccagcccg cgctgcccca acggctggcc ggactgggcg aggagcagcg ccgccgggcc 60360

gtgctcgacg tcgtacgccg caacgcggca gcggtcctcg gccacgcccg ggcgtccgcc 60420

gtggacaccg cgcgcggctt cctggacctc ggcttcgact cgctgaccgc cgtcgaactg 60480

cgcaaccggc tcaccgaggc caccggcctg cggctgtccg cgaccgccgt cttcgaccac 60540

ccgaccccgg ccgccctggc ccgccatctc ctgaccgaac tggaaccgct ggtccgggcc 60600

gcgcggagcg ccctgcccag cgcgccggac cccgacgccg acctgcgcgg cgccatcgcg 60660

gccatcccgc tcgaacgcct ccggcaggcc ggactcctgg acgaactggc ccgcctggca 60720

ggcgtcgccg taccggccaa ggacgccccg gccgagcagc acgcagcccc cgacgaccac 60780

cccgacgacc ccgcggacgg acccgaggac gacggttccg acgacctcat ggacgcgctg 60840

gacgacatga gcatcgacga cctgatcagg atcgcgcacg acgagcgccc ccgcggaaac 60900

tgaggatcac gaacagcatg agcaacacca acgaagaact cgtcgaggcg ctgcgcagct 60960

cgctgcgcga gaccgagcgg ctgcgccgcc acaaccgctc gctgacggcc gccgccgacg 61020

agccgatcgc catcgtcggc accgcctgcc ggttccccgg cggcatcgac tcgcccgagc 61080

ggctgtggga cgccgtcgcc gccggctcgg acctgatcac cggcttcccc gacgaccgcg 61140

gctgggacct cggcgtccac gaccccgacc ccgagcgggc cggccgcagc tacaccgacc 61200

gcggcggctt cctcaccggc gccgccgact tcgacccggc gttcttcggt atctccccgc 61260

gcgaggcccg cgccatggac ccccagcagc gggtgctgct ggagaccgcc tgggaggcgt 61320

acgaacaggc cggcatcgac ccgcacgccc agcgcggcag ccgcaccggc gtgttcgtcg 61380

gcacctggag ccagggctac ggcatcggcg cccgcgtccc cgaggacgcc gagggctacc 61440

tcgtcaccgg cggcgccacc gccgtcgtct ccggccggat ctcctacgtg ctcggcctgg 61500

agggcccggc cgtcacggtc gacaccgcct gctcctcgtc gctggtcgcc ctgcactggg 61560

cggtgcggtc gctgcgcgcc ggggaatgct cgatggcgct cgccggcggc gtgaccgtca 61620

tggccggccc cggggtgttc gtggagttct cccgggagcg cggcctcgcc cccgacggcc 61680

gctgcaaggc ctactcggcc gacgcggacg gcaccggctg gggcgagggc gtcggcgtgc 61740

tcctgctgga gcggctctcc gacgccgtcc gcaacggcca ccgcgtcctc gccgtcgtgc 61800

gcggctccgc cgtcaactcc gacggcgcca gcagcggcct caccgccccg aacggcccct 61860

cccagcagga ggtcatccgg caggccctcg ccgacgcccg gctcaccccg tccgacgtcg 61920

acgtcgtgga gggccacggc accggcaccc ggctcggcga ccccatcgag gcccaggccc 61980

tgctcgccgc ctacggccag gaccgccccc gcccgctgct gctcggctcc gtgaagtcca 62040

acctcggcca cacccaggcc gccgcgggcg tcgccggcgt catcaagatg gtcgaggcca 62100

tgcgccgcgg catcgcaccc cgcaccctcc acgccaccga gcccaccccc caggtggact 62160

ggtcccgcgg agccgtcgaa ctcctcaccg acaaccgctc ctggcccgac accggggcac 62220

cccgccggtc cgcggtgtcc gcgttcggcg tcagcggcac caacgcccac atcgtcctcg 62280

aacaggcccc cgagcccgcc gacaccgaca ccaccccgcg caccgcacac ccggtcgtgc 62340

cctggctgct gtccgcccac acaccggccg ccctgcgcgc ccaggccgaa cggctctccg 62400

ccggcctgcc cgacgacgcc gaccccctcg acgtcgccgc cgccctggcc accacccgcg 62460

ccgccctgcc gctgcgcgcc gccgtcctcg gcgccgacgc gactcagctg cgcgccggcc 62520

tcgccgcgct cgccgccggc gcacccgccg ccctctccgg cgaagcccgg tccgaccgca 62580

ccacggcctt cctctacacc ggccagggcg cccagcgcgc cggcatgggc gaggaactcg 62640

ccgccgccta cccggcgttc gccgccgcct ggaccgaggt ctgcgcggag ttcgacaccg 62700

tgctgccccg ccccctgcgc caggtgatca ccgaaggcgg ccccgacctc gaccgcaccc 62760

tgtacgcgca ggccgcggtg ttcgccttcg agaccgcgct gaccgcgctg ctcggctcct 62820

gggggatacg ccccgacctc gtcctcggcc actccgtcgg cgaactcgcc gccgcccaca 62880

ccgcgggagt gctgtccctg cggcacgccg tcgtcgtggt cgcggcccgc ggccggctca 62940

tggaggcgct gccggagggc ggcgccatgg tcgccgtcca ggcgagcgag gacgagatcg 63000

agctgcccga gggcgtggcc ctggcggccg tcaacggccc gtcgtcggtg gtcctgtccg 63060

gcgacgaggc cgctgtcctg gccaccgccg cccactgggc cgagcgcgga tgccgcacca 63120

agcggctcac cgtcagccac gccttccact cccaccgcat ggaccccgtc ctgaacggct 63180

tccgccgcgt gctgggcgcc gtcaccctca atacgccgcg catcccgttc gtctccacgg 63240

tcaccggcgc ccccgtcgaa gccggactgc gcgaccccga gtactggctg cgcaacgtcc 63300

gcgacaccgt ccggttcgcc gacggcgtcc ggaccctggc cgacgagggc gcggacacct 63360

tcgtcgaggt cggcccggac gccgtgctcg gcgccctcgt cgccgacgcg ctgcccgacg 63420

accccgaccg gcccgaaata ctcgccgtac ccaccgcccg cgcggaccgg cccgagcccg 63480

agaccctggt cggcgcgctc gcccgcatcc acgcccacgg cgcgcacgtc gactgggccg 63540

ccttcttcgg ctccggcgcc cgccgcgtcg acctgcccac ctacgccttc cagcaccagc 63600

actactggct ggccccggca cccgccgacg acctccccgc cgccggcctc ggcaccgtcg 63660

gccacccgct gctgcgcgcc gccgtcgaac tgcccgccga cgccaccgcg gaccacgccg 63720

cccgccccgg cgccgccgga gccgtcgtct tcaccggcac cctgtccgcc cacacgcacc 63780

cctggctggc cgaccacagc gtcctcggca cgccggtgct ccccggcacc gccctcgccg 63840

aactagccgc cgccgcgggc gaccgcctcg gctgcgccac cgtcgccgaa ctcgtcctca 63900

ccgcgccgct cgccgtcccc gcctccggcg ccgtccggct ccgcgtccac gtcgacgccc 63960

ccgaccgcga cggccaccgc gccgtcaccg tcgactcccg gcccgacgac cagctcgcgg 64020

acggcgccgc ctggacccgc cacgccaccg gacgcctcgc ccccaccggt cccgaacccg 64080

cccaggcgcc caccgcctgg ccgcccgccg acgccgaacc cctccccgtc gacggcctct 64140

acgaccggct caccgcggcc ggcttcggct acggccccgc cttccgcggc gtccgcgcgg 64200

cctggcgcct cggcaccgac ctgctcgccg acgtcgaact gcccgcggac accgacgagt 64260

cgggctttct gctgcacccc gccctgttcg acgccgccct gcacgccctc ggcctcggcg 64320

gcctggtcga gcacggcgga ctgcccttca cctggacggg cgtccgcctg cacgcgaccg 64380

gcgcccgctc cctgcgggtg cggctgaccc cgaccggccc cgacgccgtc gcgctcaccg 64440

cggccgacgc cacgggccgg cccgtcgtca ccgtcaccga cctccgcctg cgcccggcca 64500

ccgaggtccg cggcaccacc acggccgacg ccctgcacca catacggtgg gaaccccgcc 64560

cggcagctgc ggccaccgac gtcaccggca ccgacttcac cgtgttcacc ctgccggccg 64620

ccgggtccga cccgcaggcc gtccgggagg ccacgcgcgc gtccctcgcc cggttgcagg 64680

agcatctcgc cgccgtcggg ccggccggcc cgctcgtcgt ggtcacccac ggcgccgtcg 64740

ccgcagaacc cggcgacacc gtccccgacc tggccggcgc ggcggtctgg ggcctggtgc 64800

gctccgccca gtccgagcac cccggccgct tcgtcctgat cgacctggcc gcttccgagg 64860

atgccgctgt cgttccggcc gcgatcgcca ccggagagcc tcagatcgct gtacggaatg 64920

gagcgctgta cgcgccccgg ctggtgcgcg gggcttctcg gccgggtggt gaggtgccgt 64980

tcggtgcggg cgacgtggtg ctggtgaccg gtggcacggg tgctctgggc cggttggtgg 65040

cccggcacct ggtggccgag cacggtgtgc gtcggctggt gctggcgagc cggcgcggag 65100

gcgccgggga gctggtcgcc gagttgggtg agttgggtgc cgctgtggac gtcgtggcct 65160

gcgatgtgtc cgaccgggac gcgctggagc gtctggtcgc ggcccatccg ctgacgggtg 65220

tggtgcacgc ggccggtgtg ctggacgacg gcacggtgga gtcgctgacc cctgaccggg 65280

tggacggggt gctgcgggcg aaggtcgacg gtgcctggca tctgcacgag ctgacggccg 65340

ccctggacct ccgtgccttt gtgctgttct cgtcgctcgc gggtgtggtg ggcagtgcgg 65400

gtcagggtgg ttacgcggcg gcgaacgccg cgctggacgc gctggcggag caccggcggg 65460

ccgagggcct gcccgccacc tccatcgcct ggggaccctg gaccgacggc atggcgagcg 65520

gcctcgaccg ggtggacgcg gcccggctcg cccgcggtgg agtcgtgccc ttcgcccacg 65580

ccgacggcct ggagctgttc gacgccgtct gcgccaccga cgcaccgctg accgtcgccg 65640

cgcgcctgga cctcgccgtg ctgcgcgccc aggcggaggc gctgccgccc gtgctgcgcg 65700

gcctggtgcc ggcgcccgca cgccgtaccg ccgctgccgc ccggcccggc ggcttcgccg 65760

agcggctcgc cgcgctgccc gagaccgagc ggcggcgcgc cgccctggaa ctggtccggg 65820

agaccgcggc gaccgtgctc ggccacgtcg gcccggaggc cgtcgccccc gaccgctcct 65880

tcctcgacct cggcttcgac tcactggccg ccgtcgaact ccgcaaccgg ctcaccgccg 65940

cgaccggcct gcgcctggcc gcgacgatca ccttcgacca ccccacctcc gccgccctcg 66000

cacgccacct cctggacgcg gccctggacg ccggcccgtc ggccacgagc ggcgcgcacc 66060

tgcccgccgc caccgccgta cgcgccgacg agccgatcgc gatcgtgggc atggcctgcc 66120

gctaccccgg cggtgtgacc tcgcccgagg acctgtggcg actcgtgctc gcgggcgccg 66180

acgccgtcac cggcttcccc gaggaccgtg gctgggacct cacctccgtc tacgaccccg 66240

accagtcccg caccgggacg agctacaccc gcgagggcgg attcctcacc ggggcggccg 66300

acttcgacgc cgagttcttc ggcatcagcc cgcgtgaggc gctggccatg gacccgcagc 66360

agaggctgct gctggagacc tcctgggagg cgatcgagcg cgccggcatc gacccgaccg 66420

cgctgcgcgg cagcgccacc ggtgtcttcg ccggcctgat gtaccacgac tacggcagcg 66480

gcaccggcac cctcccggag ggcgtcgagg gctacctggg tctcggcacc gccggcagcg 66540

tgctctcggg ccgggtcgcc tacacgctcg gcctggaggg ccccgcggtc accgtggaca 66600

ccgcgtgctc ctcctcgctg gtcgccctgc actgggcgat ccaggcgctg cgcagcggcg 66660

agtgctcgat ggcgctggcc ggcggtgtca ccgtcatggc cacaccgggc acgttcgtgg 66720

agttctcccg gcagcggggc ctcgcccccg acggccgctg caagtccttc gccgccgcgg 66780

ccgacggagt cggctggtcc gagggcgtcg gcatgctcct cgtggaacgc ctgtcggacg 66840

cccgccgcaa cggccaccaa gtgctggccg tcgtacgcgg atccgcggtg aaccaggacg 66900

gcgcgtcgaa cggtctgacc gccccgaacg gtccctcgca gcagcgggtg atccgccagg 66960

cgctccaggg cgccgggctg acgacggccg acgtcgacgc cgtggaggcg cacggtacgg 67020

gcaccaaact gggcgacccg atcgaggcgc aggccctcct cgccacctac ggccaggagc 67080

gggacgagcc gctgtggctc ggttcggtga agtccaacat cgggcacacg caggccgccg 67140

ccggtgtggc cggtgtgatc aagatggtgc aggccatgcg gcacgggacg ctgccgcgca 67200

cgctccacgt ggacgaggcg tcaccgcacg tcgactggac cgccggcgcg gtggaactgc 67260

tcaccgagga acgcgagtgg acccggacgc gtcggccgcg ccgcgccgcc gtgtcgtcct 67320

tcggcgtgtc cggcaccaac gcccacgtcg tcctcgaaga ggcacccgag gagaccgcac 67380

cccccgtatc ggccgaactg cccctcgtgc cgctgctgct gtccggtcac acctccacgg 67440

cgctcgccgc ccaggcacgc cgactgcacg accacctcgc cgagtccggc acaccggccc 67500

tggaaacggt cggccgctcc cttgccgcgt cgcgggcgtt gttgccgcat cgtgcggtgg 67560

tggtggccgc tgatgtggcc ggtgcgcggg cttctctggc ggcgttggcc gcgggggagc 67620

cggtggaggg tgtggtgtcg ggtgctgccg ggttgccggg tgagggccgg gtggtgttcg 67680

tgttcccggg tcagggggcg cagtgggtgg ggatggcggt ggatctgctg gattcgtcgc 67740

cggtgttcgc ggggcggatg gctgagtgtg cggtggcgtt ggagccgttc gtggagtggt 67800

cgttgcgtgg tgtgctgggt gatccggtgg cgttggagcg ggtggatgtc gttcagccgg 67860

tgttgtgggc ggtgatggtg tcgttggcgg aggtgtggcg ttcgtacggt gtcgtgccgt 67920

cggctgtggt cggtcattcg cagggggaga tcgcggctgc ttgtgtggcg ggtgtgctgt 67980

cgctggcgga cggcgcccgg gtcgtcgccc tgcgcagcag ggctctgacg gcgctggcgg 68040

gcagcggggg gatggtgtcg gtcgccgccg ggccgtccgg tgtcgaggag ttgctggtgg 68100

gctgggcggg ccggttggcg gtggctgcgg tcaacggtcc tgagtcggtc gtcgtcgcgg 68160

gtgagggggt ggcgctggag gagttcctgg ctcattgcgg gggtcggggg gtgcgggctc 68220

ggcgtatcgc tgtcgactat gcctcgcatt cggtgctggt ggagccggtg cgtgaggctc 68280

tgctggctga tctggagggc gtgcggccgg gtgagggcac ggttccgctg ttctccaccg 68340

tgacggggga gtgggcggac ggcacggcgc tggacgccgg ctactggtac cgcaacctgc 68400

gcgaaccggt gggcttcgaa ccggcggtgc gcggcctgct cgactccggc cacgccgtgt 68460

tcgtggagat cagcccgcac ccggtgctga cggccgccgt gcaggagacg gcggatgcca 68520

ccgagcggac cgccgtcgtg gtcggcaccc tccgccgcga ccacgagggt cagcggcaac 68580

tgctcaccca cctcggcgtc ctgcacacca cgggcgccga catcgactgg acgggctgct 68640

tcacgggcgt caccggccgt gccgacctgc ccacctacgc cttccagcac acgcgctact 68700

ggctgacccc ctccggcccg tccgccggcg aactggccgg agcgggcctg accgccgccg 68760

ggcacccgct gctcggcgct gccgtcgacc tcgcggagga cggcggcctc gtcctgaccg 68820

gacgcctcgc cgcggacccg gccgcctgga cggccgatca cgtcgtcctc ggcaccaccc 68880

tgctgcccgg cgcggcgctc gccgaactcg ccctcgccgc cggcgacggc gtcggctgcg 68940

gcaccctgga cgaactcgtc ctcggcgcac ccctggcact gcccgaacgc ggtgccctgc 69000

acctccaggt acgggtgggc cgcgccgagg ccgaccaccg ccgtaccgtc agcgtccacg 69060

cgcgccccga ggacggtgac gcgccctgga cccggcacgc cgagggcgtc ctcgtgcccg 69120

gggacaccgc cgccggcacg ccgctgaccg agtggccgcc cgccgacgcc gaaccggtgg 69180

acgtgtccgc gctctacgac agcctcgccg accgcggcct cgactacggg ccggtcttcc 69240

gtggcgtacg cgcggcctgg cggcacggcg acgacatcct ggcggaggtc gaactgcccg 69300

cggaggccga cgagtcgggc ttcctgctgc acccggccct gctggacgcg gccctgcacc 69360

cgatcggcct cggcggcctg gtcggcgacg gcggactgcc cttcgcctgg cacggcctgc 69420

gtgtgcacgc cgtcggcgcc cgcgccgcgc gggtacggct cacccccctg ggcgacgaga 69480

ccgtcgccgt agacctcgcc gacggcgccg gcgcgcccct ggccgccatt gtctccctgc 69540

gcctgcgagc cgtcacggcc gcacaggtcg ccgccgcccg caccctcacc gacccggccc 69600

tgcacctgga ctggctcccg gtggccggcg ccgcgtccgc gccctcggac gacaccgccg 69660

tggaactgct gcgcttggac gacgcaccgc acgggacgga cgccggtccc gggggcgtgc 69720

ggcaggccgt gcgtacggcc ctcaccgcga tccaggagcg gatcgccgac gaccgggccg 69780

agcggctggt cgtcgtcacc cgcgacaccg acctcgccgg cgccgccgtc ggcggtctgc 69840

tgcggtccgc ccaggccgag cacccgggcc gcttcggcca cgtcgtgctc gacggacacc 69900

ccgactccga gcgcgccctg cccaccgcca ccgccctgac cgacgagccg tgggtcgccg 69960

tgcgcgccgg ggagacatac gtaccgcggc tcgcccggtc cggtgcgcgg ccgggcggcg 70020

acgtgccgtt cggcgccgac gacgtggtgc tggtgaccgg cggcaccggg gtgctgggcg 70080

ccctggtggc ccggcacctg gtgaccgagc acggtgtgcg caggctggtg ctggcgagcc 70140

ggcgcggagg cgccggggag ctggtcgccg agttgggcga attgggcgcg gctgtggatg 70200

tggtggcctg tgatgtgtcc gaccgggagg cgctggagcg tctggtcgcg gcccatccgc 70260

tgacgggtgt ggtgcacgcg gccggtgtgc tggacgacgg cacggtggag tcgctgaccc 70320

ctgaccgggt ggacggggtg ctgcgggcga aggtcgacgg tgcctggcat ctgcacgagc 70380

tgacggccgc cctggacctc cgtgcctttg tgctgttctc gtcgctcgcg ggtgtggtgg 70440

gcagtgcggg tcagggtggt tacgcggcgg cgaacgccgc gttggacgcg ctggcggagt 70500

accggcgggc cgagggcctg cccgccacct ccatcgcctg gggcctgtgg gcgccggcga 70560

gcgccatgac ctccggcgcc gacaccgcgc gcctggcccg gagcggcatc ctcccgctcc 70620

ccgccgaccg ggccctggaa ctgttcgaca ccgcctgcgc cgccgagacg ccgctgaccg 70680

tcgccgcccg cctggacctc acggcgttcc gcgcccaggg cacccgtatg cccgtcgtcc 70740

tgcggagcct cgcgggcccc gccgcccgcc gcacggcacg cgccggcgac gccggcacct 70800

tgcgcgaccg cctcgccgcc cagacccccg ccgaacggac ccggaccgtg ctcgacctgg 70860

tccgaggcca ggccgcggcc gttctcggac acgagtcggc cgccgccatc gccgaggacc 70920

gggccttcct cgaactcggc ttcgactccc tgaccgccgt cgacctgcgc aaccgcctcg 70980

gcacggtgac gggcctccgc ctgcccacca ccaccgtgtt cgaccacccc aaccccgccg 71040

cactgacccg gcacatcctc gccgagctgc tcggcgccac ggccggctcg gcccaggccg 71100

cccccaccgc cgtgcggacc gacgagccga tcgcgatcgt gggcatggcc tgccggtacc 71160

ccggcggagt ggcctctccc gaggacctgt ggcgcgtcgt cgccgagggc cgtgacgtca 71220

tctccccctt cccggaggac cggggctggg acctcggcgc cctgtaccac gccgaccccg 71280

accacacggg gaccagctac gcccgcgagg gcgggttcct gcacgacgcc gccgggttcg 71340

acgccgagtt cttcggcatc agcccgcgtg aggcgctggc catggacccg cagcagcggc 71400

tgctcctgga ggcgtcctgg gaggcgatcg agcgcgccgg catcgacccg accgcgctgc 71460

gcggcagcgc caccggtgtc ttcgccggcc tgatgtacca cgactacgcg gcccggctcg 71520

gcaccacccc ggagggcctc gaaggctacc tgggcatggg caactccggc agcgtcgcct 71580

ccggccgcat ctcctacacc ctgggcctgg aaggcccggc ggtcaccgtg gacaccgcct 71640

gctcctcgtc cctggtcgcc ctgcactggg cgatccaggc gctgcgctcg ggggagtgcg 71700

acctcgccct ggccggcggc gtgtccgtga tggcgacccc gggcacgttc gtggagttct 71760

cccggcagcg gggcctcgcc cccgacggcc gctgcaagtc cttcgccgcc gcggccgacg 71820

gggccagctg gtccgagggc gtcggcatgc tgctcgtgga gcgcctgtcg gacgcccgcc 71880

gcaacggtca ccgtgtgctg gccgtcgtgc gcggctcggc ggtgaaccag gacggtgcgt 71940

cgaacggtct gacggccccg aacggtcctt cgcagcagcg ggtgatccgt caggcgctcg 72000

ccggtgccgg gctcacgtcc gcggacgtgg acgcggtgga ggcgcacggt acgggcacgc 72060

ggctgggcga cccgatcgag gcgcaggccc tcctcgccac ctacgggcag gagcgggacg 72120

agccgctgtg gctcggctcg gtcaagtcca acatcggcca tgcgcaggcc gccgccggtg 72180

tggccggtgt gatcaagatg gtccaggcga tgcggaacgg cgtgctcccg cgcacgctcc 72240

acgtcgatga gccgtccccg cacgtcgact ggaccgtcgg cgcggtcgag ctgctgaccg 72300

gggaacagga gtggccgcgg caggaccgcc cgcgccgtgc gggtgtgtcg tcgttcggtg 72360

tgtccggcac gaacgcgcat gtggtgctgg aagaggctcc cgaggagacc gagggcaccg 72420

cgccggctgc gctgcccgcc gtgccctggg tggtgtcggc ccgctccgag accgcgctgc 72480

gtgcgcaggc cgcacggctc gcggactggc tgcacggcga caccgacgtc ctcggcaccg 72540

cctactccct cgccaccggc cgggcggcgc tgccgcaccg cgcggtcgtg gtcgggaccg 72600

accgggccga actctccgac ggactggccg cgctggcggc cggtcgcgcc gccgcgcacg 72660

tcgaatcggg ccgtgcccgc gacaaccgtg tcaccgcgtt cgtgttcgcg gggcagggtg 72720

cgcagcgggc ggggatgggg gcggaactgg ctgccgcgta tccggtgttc gctcaggtgt 72780

tcgcgcaggt gtgtgcggcg ttcgacggtg tgctggagcg gccgctgggt gaggtgatcg 72840

ccgagggtgg tccggagctg gatcggacgg tgtacgcgca ggcggggttg ttcgctttcg 72900

aggtggcgtt gttccggctg ctggagtcgt ggggtgtcgc gccggacgtc gtactcgggc 72960

attccgtggg tgagttggcg gctgcctgtg tggcgggtgt gtggtcgctc gaggacgcgg 73020

tgcgggtggt ggctgcgcgt gggcggttga tgcaggcgct gccgcagggc ggtgcgatgg 73080

tcgctctcga ggtgtcggcg ggtgagctgg agttgccgga gggtgtggag ctggcggcgg 73140

tcaatggtcc gtcgtcggtg gtgctgtccg gtgaggagga ggccgtcctg gctgaggcgg 73200

cccgctggcc ggaccgtcgt gccaagcgtc tgcgggtcag tcacgccttc cactcgcgtc 73260

ggatggatcc gatgctggag gacttccggc gggtactgga gtcggtggcc ttccacgctc 73320

cggagcttgt tttcgtgtcg acggtgaccg gtgctgtggt gacggacgag ttgtgtgacc 73380

cggggtattg ggtgcgcaat gtgcgggaga cggtccggtt cgcggatgcg gtggtggcgg 73440

cggaggccgg tgtcttcgtg gagttggcgc cggacgcggt gttgtcgggg ctggtgggcg 73500

agtccgtcga gggtgtgctg tgtgttcccg cgcagcgggc gggcaggccg gccgcgcggg 73560

ttctcgtgtc ggcgctgggc acgctgcaca cgcacggtgt cgacgtcgcc tggggccggt 73620

tcttcgccgg tagcggtgcc cgtcgtgtcg acctgcccac ctacgccttc caacgcgaac 73680

gctactggct ggacgccccc gcgccgcaga ccggtggcgc ctcggacgac gccttctggg 73740

ccgccgtcca gagcggtgac ctcgccggtc tgctgggtgt cgcggaggac gccgggctgg 73800

acgccgtact gcccacgctc gcgtcctggc acgacaggga acgtgccgac gcggtcgtgg 73860

acggatggcg gcacaaggtc cgctggaccc cgctccccga ggccggcggc gccgtgctga 73920

gcggccggtg gctgctggtc ggcccggagg gggacgaggc gctcgtcgcc gacgtcgcca 73980

ccgccctgcg cgagcacggt gccgaggtca cgcacctcgc gctcccgcgg gacgccgacc 74040

gggagacgac cgccgaactg ctccgcggca ccgacgacac cggtctcacc gcagtgctgt 74100

ccctgctcgc gcgggcggac cgcccggtgc acgccaccct cgccctcgtc caggcgctcg 74160

gcgacgccga agtcaccgtg ccgctgtggt gcgctacctc cgagtccgtc gccgtcgcct 74220

ccacggacgt cgtaccggaa gccgccgtcg acgcggccgg cctctggggg ctcggccggg 74280

tcgtacgcct ggaggcaccg gaccgctggg gcggactggt cgaccttccc ggcgcgctgg 74340

acgcgcgggc ccgccgccgg ctcgccgccg tcctggccgg cgccgaggac gagtgcgcgg 74400

tgcgcgagaa cggcgccttc gccgctcgtc tcgtccgagc cgccgacgca ccccgccgcg 74460

agtggcggcc ccagggcacg gtcctcgtga ccggcggcac cggcgcgctc ggcgcgcggg 74520

tcgcccagcg gctcgcggag cgcggtgcgc ggcacgtgct gctggtgagc aggcgcggtc 74580

cggcggccga cggcgcggcc gaactggtcc gcgccatcga ggcggcgggc gccacggcga 74640

ccgtcgccgc gtgcgatgtc gccgatccgg aggcggtcgc ggccctgctg gagcggatac 74700

ccgccgacgc cccgctgacc gcggtggtgc acacggccag cgtcctcgcc gacgccccgc 74760

tggacacgct caccccggac cggatcaccg ccgtcctccg ggccaaggcc gatgccgcac 74820

gaatcctgca caccgcgacg gccagcctcg acctggacgc gttcgtgctg ttctcctcgc 74880

tcgccggcac cctcggcaac cccgggcagg ccgcctacgc cgccgcgaac gccgtactcg 74940

acacgctcgc cgcgcaccgc cgggccctcg gactgcccgg caccgccgtc gcctggggcc 75000

cctgggccgg cggcggcatg ctcgacgaca cggtggcgga gcgactgcgc cgcgccggtg 75060

tcatcccgct cgaccccgag cacgccctgg tcgccctcga ccgtgccgtg gccgccgcgg 75120

acgcgcacag cgtcatcgcc gacgccgact ggaccggcct caccgccggc tccatgctgg 75180

ccgaactctc cggcccggca cccgtcgagg cgcccacggc gctcaccggt cccgaccgcg 75240

agcgcgccgc actggccctc gtccgctcct gcgccgccgc cgtcctcggc cgctcggccg 75300

ccgtggacgt cgaacccgac accgccttcc gcgagctggg cttcgactcc atggccgccg 75360

tccagctgcg caaccggctc aacgccgcca cgggggtcgt gctcaccgcg accgccgtgt 75420

tcgaccaccc gagcgcccgc gccctggcca cccacctgct cgctctggcg accggggagg 75480

gcgccggctc ggccggcgcg accgaactcc cgggtacggc cgtccacacg gacgagccga 75540

tcgcgatcgt cggcatggcc tgccgcttcc ccggcgacgt gacctcgccc gaggacctgt 75600

ggcggctgct cgccgacggc gtggacgccg tcggccccat cccggcggac cgccagtggg 75660

gacccaccga cgcctacgcc gaaggcggct tcctgcgcgg ggccggcgag ttcgacgcgg 75720

acttcttcgg gatcagcccg cgcgaggcgc tggtcatgga cccgcagcag cggctcgccc 75780

tggagaccgg ctgggaggtg ttcgaacgcg cgggcatcaa cccgcacacg gtgcgcggca 75840

cgtccgtcgg cgtcttcctc ggcaccaacg gccaggacta cgtgtccctc ctcgccggcg 75900

ccaccgaggc ccacgcggga cacatcggca ccggcaactc cgccagcgtg ctgtcgggcc 75960

gcatcgccta cgtcctcggc ctggagggcc ccgcggtcac cgtggacacc gcgtgctcct 76020

cctcgctggt cgccctgcac tgggcgatcc aggcgctgcg cagcggcgag tgctccatgg 76080

ccctggccgg cggtgtcacc gtcatggcca ccccgggcgc cttcgccgag ttctcccacc 76140

agcggggact ggcggaggac ggccgctgca agtccttcgc ggcatcggcg gacggcaccg 76200

gctggggcga gggcgtcggc atgctcctgg tggagcgtct gtcggacgcc cgccgcaacg 76260

gtcaccgtgt gctggccgtc gtgcgcggct cggcggtgaa ccaggacggt gcgtcgaacg 76320

gtctgacggc tccgaacggt ccttcgcagc agcgggtgat ccgtcaggcg ctcgccggtg 76380

ccgggctcac gcccgtggac gtggacgcgg tggaggcgca cggcacgggc acgcggctgg 76440

gcgacccgat cgaggcgcag gcgctcctcg ccacctacgg gcaggagcgg gacgagccgc 76500

tgtggctcgg ttcggtcaag tccaacatcg ggcacacgca ggccgccgcc ggtgtggccg 76560

gtgtgatcaa gatggtggag gcgatgcgga acggaacgct gccgcccacc ctccacgtgg 76620

acgagccgtc cccgcacgtc gactggtccg ccggcgcggt cgagctgctg acggaagcgc 76680

gggagtggaa gcgtgccggt cggccgcgcc gtgcgggtgt gtcgtcgttc ggtgtgtccg 76740

gcacgaacgc gcatgtggtg ctggaagaag cgccggagga gaccgaaccg gcggcttcgg 76800

acgaactgcc ggtcgcaccc tggctgttgt cggcccgctc agagaaggcg ctgctggcac 76860

aggtcgagcg gctgcgcgcg tacgtcacgg agcacccgga ggcgcggccg gccgacatcg 76920

gcctcagcct ggccaccggc cgagccgcgc tggcccaccg tctgagcgga gccggcgaga 76980

ccacggagga actgctcgcc gcccttgaca ccgcccttcc cgccgtggct cgcgaaaccc 77040

cgaccgcgtt cgtgttcgcg gggcagggtg cgcagcgggt ggggatgggg gcggaactgg 77100

ctgccgtgta tccggtgttc gctcaggtgt tcgcgcaggt gtgtgcggcg ttcgacggtg 77160

tgctggagcg gccgttgggt gaggtggtcg ccgagggtgg tccggagctg gatcggacgg 77220

tgtacgcgca ggcggggttg ttcgctttcg aggtggcgtt gttccggctg ctggagtcgt 77280

ggggtgtcgc gccggacgtc gtactcgggc attccgtggg tgagttggcg gctgcctgtg 77340

tggcgggtgt gtggtcgctc gaggacgcgg tgcgggtggt ggctgcgcgt gggcggttga 77400

tgcaggcgct gccgcagggc ggtgcgatgg tcgctctcga ggtgtcggcg ggtgagttgg 77460

agttgccgga gggtgtggag ctggcggcgg tcaatggtcc gacgtcggtg gtgctgtccg 77520

gtgaggagga cgccgtcctg gctgaggcgg cccgctggcc ggaccgtcgt gccaagcgtc 77580

tgcgggtcag tcacgccttc cattcgcgtc ggatggatcc gatgctggag gacttccggc 77640

gggtactgga gtccgtcacc ttccacgctc cgcagcttgc cttcgtgtcg accgtgaccg 77700

gtgctgcggt gacggacgag ttgtgtgacc cggggtattg ggtgcgcaat gtgcgggaga 77760

cggtccggtt cgcggatgcg gtggtggcgg caggggccgg tgtcttcgtg gagttggcgc 77820

cggacgcggt gttgtcgggg ctggtgggcg agtccgtcga gggcgtgctg tgtgttcccg 77880

cgcagcgggc gggcaggccg gccgcgcggg ctctcgtgtc ggcgctgggc acgctgcaca 77940

cgcacggtgt cgacgtcgcc tgggaccggt tcttcgccgg tagcggtgcc cgtcgcgtcg 78000

acctgcccac ctacgccttc caacgcgaac gctactggct cgcgccgccc gcggccggcc 78060

ccgtcggcct cgcgggcgtc ggtctcacgg ccaccggtca ccccctgctc ggcgtcagcg 78120

tcgagctgcc cggcacggac gcggtggcgt tcaccgggag cgtgtcgctg agcacccacc 78180

cgtggctggc cgaccacgcc atcctggggc ctgccctgct gccgggcacg gcgttcctgg 78240

acctcgccct cgcggcgggc gaacacgtcg gctgcccgcg ggtggacgac ctcgcgctgc 78300

acgccccgct ggcgctcccg gcccacggct cggtgcgcat gcaggtcagg gtcgagggga 78360

ccgaccccga cgggagccgc caggtcggca tctactcacg gcccgaggac gacgaggacg 78420

cctggaccca gcacgcgacc ggcgtgctgg cgcccgaaag cggccccgcc gccgaggcgc 78480

tgaccgagtg gccgccccag ggcgccgaac ccgtgtccgt ggacggcctg tacgacgacc 78540

tggccgccac cgggttcgcg tacggccccc tcttccgcgg cctgcgtgcc gcctgggtgc 78600

gggacggcgc cgtctacgcc gacgtcgccc ttccggacga gacggcctcc gtcgcgggct 78660

acagcgtgca cccggcgctg ctcgacgccg ccctgcacgc gctcggctgc gcgaacctgg 78720

tcgcggactg ggcggacgga cagctgccct tcgcgtggac cggcgcgcgg atccacgccg 78780

tgggcgccag ggcgctgcgc gtccggctcc gggccgagaa cggcgggatc gcgctgaccg 78840

cggccgatgc cggcggacag cccgtggccg ggatcgaagg agtccggctg cggcccgtcg 78900

gcgacgcgcg tgccgtccgt gcggcggcgg gcgcgcgccc ctgccgggtc gagtggctgc 78960

ccgtcgtacc ggcggacgac cccgaggacg acgacatcac cgtcgtcccc gtgaccggcc 79020

gtaccgccgg cggcgacgtc gtggccgagg tgcgtgccgc cgtcgccgcc gccctggccc 79080

ggctccagga atggctggcc gacgaccggt ccgagcgcct ggtcctcgtc acccggggcg 79140

ccgtcgcggc gctgccggac gaggcaccgg acccggtggc cgccgcggtc tggggcctgg 79200

tgcgctccgc ccagtccgag caccccggcc ggttcgtcct ggccgacttg gccgcttccg 79260

aggatgccgc tgtcgtttcg gccgcggtcg ccaccggaga gcctcagatc gccgtacgga 79320

atggagcgct gtacgcgccc cggctggtga ggggggcttc tcggccgggt ggtgaggtgc 79380

cgttcggtgc gggcgacgtg gtgctggtga ccggtgggac gggtgctctg gggcgggtgg 79440

tggcccggca tctggtggcc gagcacggtg tgcgtcggct ggtgctggcg agccggcgag 79500

ggggtgccgg ggagctggtc gccgagttgg gtgagttggg cgcggctgtg gatgtggtgg 79560

cgtgtgatgt gtccgaccgg gaggcgctgg agggtctggt cgcggcctat ccgctgacgg 79620

gtgtggtgca cgcggccggt gtgctggacg acggcacggt ggagtcgctg accgcggagc 79680

gggttgacgg ggtgctgcgg gcgaaggtcg acggtgcctg gcatctgcac gagctgacgt 79740

ccggactgga tctccgtgcg ttcgtgctgt tctcgtcgct cgcgggtgtg gtgggcagtg 79800

cgggtcaggg tggttacgcg gcggcgaacg ccgcgttgga cgcgctggcg gagtaccggc 79860

gggccgaggg cctgcccgcc acctccatcg cctggggacc ctggaccgac ggcatgacca 79920

cagggctgga gcgggcggac cgtgcccgta tcagccggtc gggtacccgg gccctcggca 79980

ccgaggacgg actcgccctc ttcgaccagg ccgtacgcgg cgccgacgcg ctcgccgtcg 80040

ccgcgctgtg ggacctgtcc gcgctgcgcg ccgcgcagtc gctgccgcct ctgttccagg 80100

ggctcgccgg acgaccggtc cggcggaccg tcgccgacgg cgccgggcgg ggcgccgagt 80160

gggccgaccg gttcaccggg ctgtcgccgg ccgaaagcga acgcgccgcc gtcgagtggg 80220

tccgtgagca ggcggcggcc gtgctggggc acgcgtcctc cgccgccgtc gccgctgacc 80280

gggccttcct cgacctgggc ttcgactcgc tcaccgccgt cgaactgcgc aaccggctcg 80340

ccacggcgac cggcctgcgg ctcggcacga ccgccgtgtt cgactacccc accccggccg 80400

ggctggccgc gcacctgctg gagcgcgtgc tcggcgccac gaccggctcg gcgcagccgg 80460

ctcccgccgc cgtaccggcg gacgagccga tcgcgatcgt cggcatggcc tgccgctacc 80520

ccggcggaat cacctccccg gaggagctgt ggcgcgaggt cgccgaaggc cgcgacgcga 80580

tctccgggtt cccgaccgac cgcggctggg acctgcgggc cctgttcgcc gacgaccccg 80640

ggcggcccgg caccagccac acccgcgaag gcggattcct gcacgacgcg ggcgagttcg 80700

acgccgactt cttcggcatc agcccgcgag aggccctggc catggacccg cagcagcggc 80760

tgctgctgga ggcgtcctgg gaggccattg agcgcgcggg catcgacccg gcatcgctgc 80820

gcggcagccg gaccggcgtg tacgccggcg tgatgtacca cgactacgcg gcccgcgtgg 80880

acgtcctgcc cgacggcgtc gagggctacc tcggcaccgg caactcgggc agcatcgcct 80940

ccggccggat cgcctacgcc ctcggcctgg agggccaggc cgtcaccgtc gacaccgcct 81000

gctcctcgtc gctggtcgcc ctgcactggg cggtccggtc gctgcgctcc ggcgagagcg 81060

acctcgcgct cgccggcggt gtcaccgtga tggccacccc gggtgtcttc gtcgacttct 81120

cccggcagcg cggcctcgcc accgacggac gctgcaagtc ctacggcgcc ggcgccgacg 81180

gcaccggctg gtccgagggc gtcggcatgc tcctggtcga acggctctcc gacgcccgtc 81240

gcaacgggca ccgggtgctc gccgtcatcc gcggcaccgc cgtcaaccag gacggcgcgt 81300

ccaacgggct caccgcgccc aacgggccct cgcagcagcg ggtgatccgg caggcgctgg 81360

ccgacgccag cctgcggccg tccgacgtgg acgccgtgga gggccacggc accggcacct 81420

cgctcggcga cccgatcgag gtggaggcgc tgctcgccac ctacggccag gagcgggacg 81480

agccgctgtg gctcggctcg atcaagtcca acatcgggca cacgcaggcc gccgccggcg 81540

tggccggtgt gatcaaaatg gtcgaggcca tgcggcacgg tgtgctgccc cgcacgctgc 81600

acgccgacga gccgtccccg cacatcgact gggcctccgg cgcggtggaa ctcctggccg 81660

agcaacggca gtggccgcgc acggaccggc cgcgccgggc cgccgtgtcc tccttcggtc 81720

tgtccggcac caacgcccat gtggtgctgg aacacacgga ccacggcgac caccactcgg 81780

ccgggaccgg cgaacggccc gccgtccccg tgcccgtccc cgtgccggtg gcgctgtcgg 81840

cgcgtaccga cgcgggactg cgggcccagg cggaccggct cgccgccgcg ctgaccgccg 81900

acccggacct gatcccgctc gacatcgcct actccgcggt gaccgggcgg gcccggctgg 81960

agcgccgggc cagcgtcgtc gcggcgaccc gcgaggaact gctggccgga ctcggcgacc 82020

tgggcccggc cacggtggcc ggcgccgggc acaccgcgtt cctgttcacc gggcagggcg 82080

cccagcgccc cggcaccggc gaggaactgg ccgccgccca cccggtgttc gccgccgcct 82140

acgccgaggt gtgcaccgcg ttcgacgcgg ttctcgaccg gccgctgcgc gaggtcgtcg 82200

ccaccggcga cggtctcgac gacaccggat acgggcagcc ggcggtgttc gcgctggagg 82260

tcgcgttcgg acggctcttc gagtcctggg gcgtcgtacc ggacttcctg ctcggtcact 82320

ccgtcggcga actggccgcc gcccatctgg ccggcgtgtg gtcgctgccc gacgcggtgc 82380

gcgtcgtcgc cgcccgcagc cggctcatgg ccgccctgcc ggcgggcggc gcgatggccg 82440

ccgtcgaggc gtccgcggac gaagtggccg ccgaactcgc cgacggcgcg gtgctcgccg 82500

cggtcaacgg cccccggtcg gtggtcgtct ccggggcacg ggacgcggta ctggccaccg 82560

ccggtctgtg ggccgcgcgc ggctgccgca cccgggaact gaaggtgtcg cacgccttcc 82620

actcgcccct gatggagccc atgctcagcg acttcgcggc ggccctggcc gacgtggagt 82680

tccgcgcgcc gcggatcccc ctggtgtcca cggtgaccgg cgccgtcgcc ggcgacgagc 82740

tgtgcacccc cggctactgg gtccggcacg tccgggacac cgtccggttc gccgacggcg 82800

tccgcgccct cggccaggcc ggcgtggaca ccgtcgtgga actcggcccg gacggcgtcc 82860

tcaccgcgat ggccgctccg ctgctgccgg acaccgccgt ggccctgccc acgctgcgcg 82920

ccggacgccc cgaggcgccc gccgtcgccg ccgcgctcgg cgccctgcac gaccggggca 82980

cggcggtgga ctggccggcc ttcttcaggg gcaccggagc ccggaccgtc gaactgccga 83040

cgaccgcgtt ccagcgcacc cgctactggc tggagtccgc cgcacggacc ggcgacctct 83100

ccgccgccgg gctcgccgcc gccgggcatc cgctgctcgg ggccgccgtc gactcgcccg 83160

acgggatgct gctcaccggc cggctcgaca cggccaccca tccctggctg gccgaccaca 83220

ccgtcctgga caccgtcctg ctgccgggta cggccttcgt ggaactcgcc cgcgcggccg 83280

gcgaacgcgt cggcctgccc cgggtacgcg aactcaccct ggccgcaccg ctcgtactgc 83340

ccgcggacgg ctccgtcctg gtccaggtgc acgtcggcgc cgccgtcgac ggcgagcggc 83400

cggtcaccgt gtccgcccgc accgacgacg gccaggactg ggcacggcac gccaccggcg 83460

tcctcgcccc cgcggcgccg gcgcccggcg ccgacccgct gccctggccg ccgcgggacg 83520

ccgaaccggt ggcgacggcc ggccactacg acgagctggc cgccgccggc ctcggctacg 83580

gccccgcctt ccgcgccctg cgcgcggtct ggcggcgcgg tgacggcccg gccgccgagg 83640

tgttcgccga gatcggtccc gcgccgggca ccgatcccgc gggcttcggc gtgcacccgg 83700

cgctgctcga cacggccctg cacgcggccg ctgtcggcgg actcggcgtc gcgggcgtgc 83760

cgttcacctg gaacgaggtg gcggtgcaca ccgccggcgc ccgctccctg cgggtccgga 83820

tcgccccgga cggaagcggc gggctgtccc tgcgcgccac ggacgacgag ggccgcgccg 83880

tgatcgacgt cggagcgctg cggctgcgtg cgatcgcccc ggaggacctg cgcaccgcgc 83940

cggccgccgc cgaggcgctg ttcgacctgg actgggcgcc cgtctccgtg gcgcggggcg 84000

cgcggcccgc cggacgctgg gcggtgctcg cccccgccga cgcggagctg accgacgtac 84060

tgggagccgg gatcgagacc gtcaaggacc tcgcgacggt gccggacgac ctggacatcg 84120

tgctcgcggc cgtgtccgac aggggcgggg ccggggtcga tgcgcccacg gctgcctccg 84180

tcggcgccgc cgccgagacc gacgtgcccg aggccgtccg ggccatcctg cgcggcgccc 84240

tcggcctggt gcagacatgg gtggcggggg agtccgccgc ccggctggtc ctgctcaccc 84300

gcggcgcggt ggcgctcgcc ggtgagcggc ccgacctggc cggtgccgcc gcctggggcc 84360

tggtgcgcgc ggcgcagtcc gagcaccccg gccggctcgt gctggccgac gtggacgacg 84420

acccggcgtc cctggccgcg ctgcccgccg ccctggcgac cggcgagccg cagctgctga 84480

tccgcgccgg tgcggtccgc gccgcccgtc tggtccgcgc cgcccccgcc gaacccgccg 84540

tacccgccgc cccgttgggc gcacgcggca cggtgctggt caccggagcc accggcagcc 84600

tcggcaccct ggtggtccgg cacctggtcg ccgagcacgg cgtccggcgg ctgctgctgg 84660

tcagccggca gggacggcag ccggagccgg ccgcggaact cgccgcggcc ggagccgaag 84720

tccgcttcgc ggcctgcgac gtcgccgacc gggacgcgct ggccgcgctc ctggcgagcg 84780

tcgaccccga gcaccccgtg acggccgtcg tgcacgccgc gggcgtgctc gacgacggcg 84840

tcgtcgcggc gctcaccccg gaccggctcg acaccgtcct gcggcccaag gcggacgccg 84900

cctggcacct gcacgaactc accggcgagc tggacgcgtt cgtgctgttc tcgtccgccg 84960

cgggactcct cggcgcaccc ggacaggcca actacgcggc cgccaacgcg ttcctggacg 85020

ccctcgcggc gcaccgccgg gccgccggcc tgcccgcggt gtcgatcgcc tggggcccct 85080

gggccgacgg catggccgca cagctcgaca cccggcgcgc ctcccgggcc ggcctgctgc 85140

cgctcgacgc cgcactcggc ctggccctgt tcgacaccgc ccgtaccggg gcgccgaccg 85200

ccccgctcgc cgcccgtctc gacctgccgg gactgcgggc cgcggcggcg gaactcccgc 85260

ccatcctgcg gaccctggtg cccgccccgg cccggacggc gcccgagccg gccgagccgc 85320

tcgcggacac cctggccgcg ctgcccgccg aggagcggga acgccgggca ctcgacgccg 85380

tcctgcggca caccgccgag gtgctcggcc acgccacggc cgacggcgtc gaccgcgaac 85440

gcggcttcca gcagctcggc ttcgactccc tgatgtccgt cgagctgcgc aaccggctgg 85500

gcgccgccgc gggcctgcgg ctgcccgcca cggtgatctt cgatcacccc acgcccgccg 85560

cgctcgcggc ccacctggtg gccgaactgg ccccgggccg ccccaccgtg gcgtccctgc 85620

gcaccagcac gctcgccgaa ctggaggccg cggcgaacga gttcgccggc gaccccgagc 85680

tgcgcgaggg cctgcgcacc cggttgcgcg cactgctgcg caccctggac gacccggcgc 85740

cggacgagcc gctcgaggag accggcgagg agagcctcgc cgaactgctc gacctcgccg 85800

accgggagct gggggacttc tgatgaccga gcacgaccgc acgaccgacc gcgccaccgc 85860

acgaggagcc accgcgttgt cccaggaaca gaccgacgac cgcaaggtcg tggagaccgt 85920

caggcgtctg accaccgacc tgcgccgcgc caagcagcgg ctgcgcgagg cggaggaccg 85980

ggcgcacgag ccgatcgcga tcgtcggcat ggcctgccgg taccccggtg gcgtcggctc 86040

gccggaggac ctgtggcggc tcgtcctgga cggccgggac gccatgggcg ccttccccac 86100

cgaccgcggc tgggacctgg cggccctctt cgccgacgac cccgagcgct ccggcaccag 86160

ccacacccgc gagggcggat tcctgcacga tgcgggcgag ttcgaccccg gcctgttcgg 86220

gatcagcccc cgcgaggcgc tcgccatgga cccgcagcag cggctcctcc tggagaccgc 86280

ctgggaggcc gtcgaacgcg ccggcatcga ccccacctcg ctgcgcggca gccgcaccgg 86340

cgtctacgcc ggcgtgatgt accacgacta cggcaccggc gccgaccctc tgcccgaggg 86400

cgtcgagggc tacctcggcc tcggcaccgc gggcagcgtc gccagcggcc ggatcgccta 86460

cacactgggc ctggagggcc cggccgtcac cgtcgacacc gcctgctcct cgtccctggt 86520

cgccctgcac tccgctgtcc gggcgctgcg cgccggggag tgcacgatgg ccctggcggg 86580

cggcgcgacc gtgctgtcca ccccggccgt cttcgtggac ttctcccgtc agggcggact 86640

cgccgccgac ggacgctgca agtcctactc cgccgaggcc gacggcaccg gctggtccga 86700

gggcgtcggc atgctcctcg tcgaacggct cggcgacgcc gaacggctcg gccaccccgt 86760

cctcgccgtg ctgcgcggct cagcggtcaa ccaggacggt gccagcagcg gcctcaccac 86820

ccccaacggg ccggcccagc agcgcgtcat ccggcaggcg ctcgccgacg cccggctcac 86880

ccccgccgac ctggacctcg tggagggcca cggcaccggc accccgctcg gcgacccgat 86940

cgaggtgcag gccctgctcg ccacctacgg ccaggaccgc gccgaaccgc tctggctggg 87000

ctcggtgaag tccaacatcg gccacaccca ggccgccgcc ggcgtcgccg gagtcatcaa 87060

ggccgtcctg gccctccggc acggtgtact gcccggcacc gcccacctga ccgagccgac 87120

cccgcaggtc gactggaccg ccggcgccgt ggaaccgctg cgggagacgc gcgcctggcc 87180

cgagaccggc aggccgcgcc gcgcggccgt gtcctcgttc ggcatcagcg gcaccaacgc 87240

ccacatcgtc ctggaacagg cccccgcccc cgcggcgccg caggcggccg gagcccaggc 87300

gcccgcggcg ccgcggcccg tcgggaacca ggccaccgcc gcgccgaggt ccatggagga 87360

ccggaccgcc gccgcgccta gcgccggcgg agacccgacc ctcaccgcgc cggccccctc 87420

cgcgccccgc cccgcccccg ccgccctccc cgtcccgctg tccgccgcga ccgagcccgg 87480

tgtccgtgcc caggccctcc ggctggccgc ccacctcacc gagcaccccg aactcgcccc 87540

gcaggacatc gcgttcagcg ccgccaccac gcgcgccgcg ctggcgtccc gggccgtcgt 87600

gctcgccgac gaccgggccg ggctgctgga cgccctcacc gcgctggccg agggacggcc 87660

cggccccgcc gtcgtcaccg gcgccgccgc ggccggcgcg cgccggatca ccttcgtctt 87720

ccccggccag ggcgcccagt gggccggcat ggccgtaccc ctgctggaga cctcgccggt 87780

gttcgcggcg aagtgggccg aatgcgcccg cgtgctcgcc ccctgggtgg actggtcgcc 87840

cgacgaggcg ctgcgctcac cgcaggcact ggaacgggtc gacgtcgtcc agcccgtgct 87900

gtgggccgtc atggtcagcc tcgccgagct gtggcgggcg gcgggcgtac ggcccgacgc 87960

cgtactcggc cattcgcagg gcgagatcgc cgccgcctgc gtcgccggcg ccctgtccct 88020

ggaggacggc gccaaggtcg tcgcgctgcg cgccaaggcc ctgctcgcgc tcgccggccg 88080

cggcggcatg ctctccgtcc cgctgcccga ggcggaggtc cgcgcccggc tcgacagccg 88140

gcccggcctc ggcatcgccg ccgtcaacgg gcccgccacc gtggtggtct ccggcgagac 88200

ggccgccctc gacgaggccc aggccgcctg ggaggccgag ggcgtccggg tgcgccgcat 88260

ccccgtcgac tacgcctccc actccccgca cgtcgccgag gtgcaggacc gcctcgccgc 88320

cgacctcgcc ggcatcgccc cgcgcccggc cgaggtgacc ttcctgtcca cgctcaccgg 88380

ggaacccttc gacaccaccg gactcgacgc cggctactgg taccgcaacc tgcgcgagca 88440

ggtccgcttc gaggcggcca cccggcgcgc cctggagcag ggccaccgcg tgttcatcga 88500

ggtcggcccg caccccgtgc tcacgctcgg cgtccagcag accgccgagg ccatggacgt 88560

gcccgccgag gcgatcgcca ccctccgccg cgaccagggc gacctgctcc gcttccgcac 88620

cgcgctcgcc gaggccgccg tcctcggcgc ccccgtcgac tgggccgccg aactcgcccc 88680

gtacgcgccc cgccgggtcg acctgcccac ctacgccttc cagcgcgagc gctactggct 88740

caccccgcag cggcgcgccg ccctggccgc cagcaccggc accgacccgt gggacggccg 88800

gttctgggac atcgtggacc gcgccgacgc cgaggaactc tcccgcgcgc tcggcgtcga 88860

cacggacgac ccgctcaccg cggtcgtgcc ggccctcgcg cgctggcgcc gcctcctgcg 88920

cgagcggtcc gccgtggaca gctggcgcta caccgtcgcc tgggagcggc tcgccgtccc 88980

cgacagcgcc cggctgaccg ggccgtggct gctcgtcgta cccgagcccc gggccggtga 89040

cgccctcgcc gcgcggtgcg ccgccgcgct caccgggcac ggcgccgagg tcaccacgct 89100

caccctgggt gcggacgaca ccgaccgcgc ggcgctcgcg gcccggctga ccggcctccg 89160

cccggccggc gtgctgtccc tgctcgccct ggacgacgcc ccgcaccccg cacacccggc 89220

cctgcccacc ggactggccc tgaccgtcgc cctcgtacag gccctcggcg acgccggcgt 89280

caccgcgccc ctgtggtgcg ccacccgggg cgccgtcgcc accggccccg ccgaccccgt 89340

caccgcaccc gtccaggcgc agatctgggg cctgggccgg gtcgtcgcgc tggagcaccc 89400

cgaccgctgg ggcggcctga tcgacctgcc cgccgagtgg gacgaccgcg ccgcaggccg 89460

gctggccgcc ctgctcgccg caggcggcga cgaggaccag acggtgctgc gcgccaccgg 89520

cgtgtccgcc cgccgcctga cccgcgcgcc gctcggcacg gagcccgccc cccggccctg 89580

gcggtgcgcc ggcaccgtcc tgctcaccgg cggcaccggc gccctcgggc cgcacctggt 89640

gcgctggctg gccggacagg gcgccgagcg catcgtgctg cccggccgcc gcggcgccga 89700

cgccccgatc gcggccgagc tgaccgccga actggccggc accggcaccg aactccactt 89760

cccggtctgc gacgtcaccg accgcgcaga actggccgcg ctcatcgacg gactcgacac 89820

ggccggcacc ccggtgcgca gcgtcctgca cgccgccacc gccctcgaac tgcgcccgct 89880

ggccgacacc cccgtcgaca cgttcgccgg ccagacggcc gccaaggtgc tcggcgcccg 89940

gcacctcgac gaactcttcg ccgaccggga cctggacgcc ttcgtgctgt tctcctcggt 90000

cgccggcgtc tggggcagcg gactgcacgc cccctacgcc gccgccaacg cctacctcga 90060

cgccctcgcc gaggaccgcc gggcccgcgg cctgaccgcg acctccgtcg cctggggcat 90120

ctgggccgcc gtcaacgagt gggacggcgt gaacgcggac gtcgaccccg aacgcgtcgg 90180

ccggcagggc ctgcccttcc tcgacccgga cctcgccgtc gccggcctgc ggcaggcgct 90240

cgaccacgac gagacgaccg tggtgatcgc cgacgtcgac tggacccggt tcgtcccggt 90300

gttctgctcc gcgcgccgcc ggccgctgct ggagtccgtg cccgaggccg ccgccgtcct 90360

gcgcaccgcc accgtcgaca ccggaacggt gtccgcgctg cgcgagcggc tcggctccct 90420

gcccgaggcc ggccgccggc gggccgtcac cgaactggtc cgcgagcacg ccgccgccgt 90480

cctcggccac gactcgcccg ccgcgctccc cgccgaccgc gccttccggg acgtcggctt 90540

cgactccatc accgcggtcg agctgcgcaa ccggctccgc tcggccaccg gcctggccct 90600

gcccgccacc ctcgtcttcg accacccgtc gcccaccgcg ctggccggcc acctgctcgc 90660

gctcgccttc gacaccgccg cggcggacct cgccgcgccc gccgcccgcg ccgccgacga 90720

tgacgacgac ccgatcgccg tcgtcggcct cagctgccgc tacgccggcg gcgtcgcctc 90780

cccggacgag ctgtggcggc tcgtcgtggc cggtcaggac gcggtgggcg ccctgcccac 90840

cgaccgtggc tgggacctcg actcgctcta cgactccgac cccgacgccc gcggtcgcag 90900

ctacgtccgc caaggggcct tcctcaccga ccccgccggc ttcgacgccg ccttcttcgg 90960

catcgccccg gcggaggccc gggccaccga cccgcagcag cggctgctcc tggaagccgc 91020

ctgggaggcg ttcgagcacg ccggcatcga cgccaccggc ctgcgcggct cgcgcgtcgg 91080

cgtcttcgcc ggcgccaacg tcggcgacta cgcctccagc cgcggccctg gcgccggcgg 91140

ctccgacgga cagctgctca ccggcaacgt ccccagtgtg atctccggcc ggatctccta 91200

caccttcggt ttcgaggggc cggccgtcac cgtggacacc gcctgctcgt ccgccctggt 91260

cgccctccac ctggcctgcc ggtcggtgcg cggcggcgag agcgacatgg ccctggccgg 91320

cggcgtcgcg ctcatgtcca gcccggccgc cctgatcggc ttctccgcgc agcgcggcct 91380

gtccggcgac ggccgctgca aggccttcgc cgacgccgcc gacggcaccg gtctcgccga 91440

gggcgtcgga ctgctgctgg tggaacgcct ctcccgggcc cgcgcccagg gccaccgcgt 91500

cctcgccctc gtacgcggct cggcgatcaa ccaggacggc gcctccaacg gactcaccgc 91560

ccccagcgga cccgcccagc agcgcgtcat caccgccgcg ctcgccgacg ccgggctgcg 91620

gcccgccgac gtcgacgccg tggaggccca tggcaccggc acccgcctcg gcgacccgat 91680

cgaggcccag gccctgctcg ccacctacgg acaggaccgc gccgaaccgc tctggctcgg 91740

ctcggtgaag tccaacatcg gccactccca ggccgcgtcc ggcgcggccg gcgtgatcaa 91800

gaccgtgcag gcgctgcggc acggcctgct gcccgccacg ctccacgtgg accggcccac 91860

cacccaggtc gactggaccg ccggcgccgt cgaggtgctg accgaggccc gggactggcc 91920

ggccgtggac cggcctcggc gggccgccgt gtcggcgttc ggcctgtccg gcaccaacgc 91980

gcacgtgatc ctcgaacagg cccccgccga agacgcccac ccggcccccg aaccggcccc 92040

gggcgaggac tcccacccga cccccgaaac ggccccaggc gaggacgccc cgcggaccgc 92100

gcccgagccc gcgcggcccg tggtgtggcc ggtgcacggc cgtacccggg acgccctgcg 92160

cgcccaggcg gcgcggctgc gcacccacct ggagacccgc cccgacgccc gcccggccga 92220

cgtcggctgg accctcgcgg ccggtcgggc cgtgttcgac caccgcgccg tggtgctcgg 92280

cgccgaccgc gccgagctgc tgcgcggact cgacgccgtc gccgccggca cccccgaccc 92340

cgcggtcgcc gacggcgcgg cccagggcgc cgaccgggcc gtgttcgtct tccccggaca 92400

cggcgcccag tggcccggca tggcccggcg gctcttcgac gacttcccgg tgttccggga 92460

aagcgtgctc cagtgcgccg acgccttcgc cgagttcgtc gactggtccc tgctcgacgt 92520

gctccgcgac gaggagggcg cgccgccgct gcaccgcgtg gacgtcgtcc agcccgccct 92580

gttcaccatg atggtgtcgc tggccgcgct gtggcgctcg tacggcgtgg agccgtcggc 92640

cgtggtcggc cacagccagg gcgagatcgc cgccgcctac gtggcgggcg ccctcgacct 92700

gcgggacgcc gcccggatcg tcgccacccg cggcaaggcc tggctcaccc tggccggcac 92760

cggcggcatg gcctccgtgg cgctgccccg cgccgaggcg gccgagcggc tgcgcccgtt 92820

cggccaccgc ctggacatcg cggccgtcaa cgacccgcgc tcggtcaccg tcgccggcga 92880

cctcgacgcg ctggaggagt tcctgaccgg cctggagacc gagggcgtcc gcgtccgccg 92940

ggtccgccag atcgtcggcg cgggccacac cgcgcacgtc gacgcgctgc gcgaccagct 93000

gatcgagacc ctcgcgccga ccgcgccgcg ctccgcgccg atcgcgttct gctccaccgt 93060

caccggcgga ctgctcgaca ccgccggcct cgaccaccac tactggtacc gcaacgcccg 93120

ccggacggtc ctgttcgaac aggcggtccg cacgctggcg gagcagggct acggcccctt 93180

cctggagatc agcgcgcacc cgatgttcac cgtcgccgtg caggagaccc tggaggacgc 93240

cggcgtcggc gccgcggtcc tcgccacgct gcgccgcgac gagggcggcc ccgaccggtt 93300

cctgcgcgcc gccgccgagg cccacacggc cggcgtcacc gtcgactggc gcccggcctt 93360

cgccggcgcc ggagcccgta cgaccgacct gccgacgtac gccttccagc gcacccggca 93420

ctggctggag ccgggcggcg acagcggcga cgtgaccgcc gcaggcctgg caccggccgg 93480

ccatccgctg ctgggagccg tggtcgaact cgccgacgga gccatggtcc tgaccggccg 93540

gctctccgcc accgcgcagc cctggccggg gcccgaggcg ccggacgccg cgctggtcga 93600

cctcgtcctg gccgccgccg accgggccga ctgcgcgggc gtcggcgaac tgaccgtgta 93660

cgaaccgctg atggcgccgg ccggcggcgc cgacacccgg gtgaccgtcg gcgcacccga 93720

cgcggtgggc agccgcacgg ccgccgtcca cacccgcacc gcggacagcc cgtgggtgcg 93780

ccacgccgag gccacgctcg tcgcccggcc ggcccccggc gagtccctga ccgactggcc 93840

gccggccggc gcggaaccga tcgacccgcc cgcggaggcg ggccgcgccg gtgtcgccgt 93900

gaccgccgcg tggcagcgcg gtgacgacct tttcacagag gtggcgctcg acgacggcgc 93960

cgccgagcgg gccgacgcgt tcgcgctgca tccgctgctg ctggacgccg ccctgagccc 94020

gctgctcgac ggcgagctga cgcccaccgc gtggtcgggc gtccgcctgc acgcgaccgg 94080

ggcgaggacc ctgagagcgc gcgcggaacg catcggcccc gacaccgtgg cgctcaccct 94140

ggccgacccc gagggcggcc cggtgctgac cgccgacacc gtccgtctcg ccgccgcgcc 94200

cgccgcggga gccggccggc gcaccggcaa ggacgccctg ttccggatgg agtgggtgcc 94260

ggcgccgctc gccccggcca cgcccggccg ctgggccgtc ctcggggccg acccgctcgg 94320

cgccgccgac accctgcgct ccctcggcca caccgtgcac ggcgccgacg gcccggccgg 94380

cctgaccgag gtgcccgacg cggtgctgat caccgccgtc ggcgccccgg gcgaggcgcc 94440

cgccgaggca cgcaccgtgc tgcacggcac gctggcggcc ctccagaccc tcctcgccga 94500

cgaccggttc accgccgtac cgctcgtggt cctcacccgg ggcgccgccg ccgaccgggc 94560

cgacgacctc gccggcgccg cggcctgggg cctggtgcgc tccgcacagt cggagcaccc 94620

gggccgcttc gtcctggccg acatcgacga cgacccccgc tcctggcggg cgctgggcgg 94680

cgtaccggcc accggggagc cgcagctggc gctgcgggcc ggtgcggcca ccgtcccgcg 94740

gctggcccgg ctcgccccgc cggacgcccc ggcgccgtgg gacccggacc gcaccgtcct 94800

cgtcaccggc gcctccggag acctcggcgc gctggtggcc cggcacctgg tggccgccca 94860

cggggtacgg cacctgatcc tcgcctcccg gcgcggcccc gccgccccgg gcgccgccgg 94920

actcggcgcc gagctgcggg cctcgggcgc cgccacggtc acgatcgcgg cctgcgacac 94980

cgccgaccgc aaggcactcg cggaactgat cgccgccgta cccgacgagc acccgctcac 95040

cgcggtcgtg cacagcgctt ccgtcctcga cgacggggtg atcgccgccc tcgacccgga 95100

ccggctggac accgcgctgc gcccgaaggc cgacgcggcc tggcacctgc acgaactgac 95160

ccggcatctg aacctgtccg ccttcgtgct gttctcgtcc gtggcgggca ccttcggcgg 95220

cctcggccag ggcaactacg cggcgggcaa cgccttcctg gacgcgctgg cccgccaccg 95280

ccgcgcccac ggcctcccgg cgacctccgt ggcctggggc tggtgggcgg aacgcgccgc 95340

gaagagcggc cacggcactg ccgccgaggc gcccgtcgcc gtcaacggca tgaccccgct 95400

gaccgaggac cacggcctcg ccctgttcga cgcggcctgc cgcggcgacg agccgttcgt 95460

ggtggccggc gcgctgcacc tgcgctcgct gcgcgccgcg gccgacgaac tgccggcgcc 95520

gctgcgcggc ctcgtgcggg cgcccgcccg caaggccgcc gcccaggccg ccgggaccgg 95580

cacgcccacg gtggccgggg aactggccgg ccggccgccc gcgcggcgcg aggcgttcct 95640

cgtggacctg gtgcgcgacg agaccgcgct cgtcctcggc cacaccggcc gggacgacgt 95700

accggcccac caccggttcc tggaccaggg attcgactcc ctggcggcgc tgaagctgcg 95760

caaccggctc gccgccgcca ccggactgcg gctgccgccg acgctcgtct tcgaccaccc 95820

gaccccgacg gagctggccc gccatctgct ggccgaactc gtcccgccgg cggacgcgga 95880

accgcccgcg ttcgacgacg aggacgccgc cacggccgtc ctggtcctgg aggaactcgg 95940

ccagttggac gaggccatca cacggctgcc cgccgacggg cccgaacgca cgcgtatcgc 96000

gggcctgttg acagacctgc tggccaggtg gggtcgatga ggttgcgcac aacactggcc 96060

acaggccctg gaccgtccta tagcatcacc gtgatcgcta cggcaggccg ggccgaccac 96120

cgccccggcc gcaccgcgcc acgggggcgg gtacgactga gggcgatcga tacgcgtgag 96180

caggcaggca cgtccgccgc acgcacgcac acaaggggga actgatggag aacgttcagg 96240

cctgggttct gcccgccggg ccgcaggaca ggtccggcgg cccggtgcgc ggcgagctga 96300

ccctcacccg cattccggtc gcctccccgg gcgagcacga ggtactggtc gagtccctcg 96360

tcggctgctg ggaggccaac atggagcacg ccctcgcgcg cagccccgtc gacatcgtcc 96420

ggcagcgcgg cgaggagcag atcgtcatcg gcacctgcgg cgtcgtccgc gtgctcagca 96480

ccggttcggc cgtgcgcgga ctgcgtgagg gccaggaatg cctgtggatc ccgttcggcc 96540

acatcgaccg caacggctac gcggagacca tctgcgccta cgacgcgccg ggttcgcccg 96600

gtctgctggc cgagcgcatg gtcgtccccg ccgaccggct cgtgccgctg ccggccgacg 96660

gacgctaccc gctggagcgc tgggcgccgt tcgcccggta cttcaccgcg tgggacaact 96720

ggcgggtcac cagccggtgc tggcgcagcc aggtcgacga cgcctgggac gagcagccgc 96780

tcgtcctcgg ctggggcggc ggggtcgtct tcgccgaact cgaactggcc cgccgcgagg 96840

gcttccgcac ggcgatggtg tccggccgcg agagccggct gaaggagatc gccgcgtccg 96900

gcgcgatcgc cgtcgaccgc cgtgagttcc gcgacctgga ctacctccgg gccaaggagt 96960

ccaccgaccc cgacgccatg gaccgctacc gcgcctccga ggcccggttc ctggagatcg 97020

tcggcgaact ctccggcggc cacggcgtct cggtcttcct ggacaacctc ggcggcggcc 97080

tgcaccgggc cacgatgaag agcctcgccc gcgagggcgt ggtgtccacc gtcggctgga 97140

agaccggcat gcggctgtgg aacctgcgcg ccaccgagtg catcagccgc cacatccacg 97200

tccacaccca cgcctggcgc caccaggacg ccccccgcat ccgtgacgtc atgcaggaga 97260

ccggctggct gcccgacata gccgacgacc cggtgaccgc gttcgcccgc gtccccgaac 97320

tggccgactc ctaccgccgg gacgacctcg acacctactt cccgctgttc tccggcgcgg 97380

ggaggggctg acccgatgag cccgcgttcc tacgacgtgg cggtcgtcgg catggcctgc 97440

gcgttccccg gtgcaccgga cctcgaccgg tactgggcca acgtggtggc cggtgtcgac 97500

tccgtcaccg aagtaccccc ggaccgctgg ccggccgacc gccactgggg cggcgccgac 97560

gcggcccccg gcgagcagtc gccgtcgaaa tggggcggat tcctgccgcc cctgcccttc 97620

gacgccctcg cccacggcgt cccgcccaac tccctcggcg gcatcgagac cgcccagctg 97680

ctcgccctgc acaccgccga ccgggcactg gccgacgccg ggtaccccga gcggccgttc 97740

gaccgggaga ccacctcggt gatcttcgcc gcggaggcgg gggccgacct cgcctccgcg 97800

tacgcggcgc gctcgatgct cggccagcac ctggagtcgg tgccggagga actggacgcc 97860

cggctgcccc ggctgaccga ggactccttc cccggcaccc tcgccaacgt catcgccggc 97920

cgcatcgcca accggctcga cctcggcggc cccacctaca ccgtcgacgc cgcctgcgcg 97980

tcctcgctgg ccgcgctcga ccaggcctgc aaggaactcc tcgccgaaac aagcgacatg 98040

gtgctgtgcg gcgccgtcga cacccacaac gcactccacg accacctgct gttcggctcc 98100

gcgcgggcac tctcgcccac cggccgctgc cgcgccttcg acgcctccgc cgacggcatc 98160

gtcctcgccg aaggcgtcgc ctgcctcgtg ctgaagcggc tcgcggacgc cgaacgggac 98220

ggcgaccgcg tctacgccgt gatcaaggcg gtcggcgcgg gcagcgacgg ccgcggcctc 98280

ggcctgaccg cgccccgccc cgagggccag cggcgcgccc tggaacgcgc ctacgccctc 98340

gcgggcctgt ccccccgcga ggtcggcctt gtcgaggcgc acggcaccgg cacggtgctc 98400

ggcgaccgca ccgaactgga gacgctcacc gaggtgttcc gcacggccgg cgccggtccc 98460

ggagcatgct cgctcggctc ggtgaagtcc ctcatcggcc acgccaagtg cgcggccggc 98520

atggcgggac tgatcaaggc cgtactcgcc gtgcacaccg gcgtccggcc ccccacccgc 98580

ctcaccgagc ccaacgcggc ctgggacccg gcgaccagcc cattcgcctt cgacaccacg 98640

gcccgcccct gggccgagcc ccgacgcgtc gcaggagtca gcgcgttcgg cttcggcggg 98700

gtcaactacc acgccgtcgt ggccgccccc gccccggacc cgtcgcacga gagcgccccg 98760

aggtccgccg gactgctcct gttccgcggc accgaggacg aggtccaaac cgccctgcgg 98820

gacctggccg gccggctggc cgagggcacc gtacggccgt ccgccctcac cggcgtcacc 98880

ggcgccggcc cggcccgggt cgccctcgtc accgagggcg ccgccacgct gggagaacag 98940

ctcgacctgg ctctgcgcct ggaggaccgc cccgagcgcg gcgtccacgt gggagacggc 99000

gcccggcagc ccgtcgcgct gctcttcccc ggccagggca gccagcggcc cggcatgctg 99060

gccgatctct tcgtcgcctt cccgcgcctg caccggttcc tgcgcgccga accggcactg 99120

accggcgccc tcttcccgcc cgccgccttc ggcggcacac ccccgcgcct ggtcgacacc 99180

cggctcgccc agcccgccct cggtctcgga gcactcgccg ccctggacct cctgcgggcc 99240

tgcggcgtcg aacccgacat gaccgccggc cacagctacg gcgaactgcc cgccctcgcc 99300

gccgccggcg ccctccccga gacggccgta atgccgctca gcggcgcccg ggccgaggcc 99360

atgcacaccg cggcgggcca ggaccccggc gcgatggccg cggtcgccgc gcccgccggc 99420

acggtcgccg aactgctcgc ccggcacggc ctggcggacg acgtcacccc ggccaaccgc 99480

aacgcccccg gccaagtggt gctcgccggc tccgccgccg ggatcgacgc cgccgtcgcc 99540

gtactccggg aggccgggca caccgccaag cggctcccgg tcgcggcggc cttccacagc 99600

ccgcggatgg ccccggccgt cgacgccttc gccggcgcgc tggccggcca cgacctgcgc 99660

gcgccccggc tgccggtctg gtcgggcgcc accgcccggc cgtacccggc ggaccccgag 99720

gcgatacgca cccagctcgc cggcgcactc gcccgcccgg tgcggttcgc cgagcagatc 99780

gaggacatgt acgcggccgg cgtccgcgtc ttcgtggagg ccggcccggg cacggtcctg 99840

acccgcctgg ccggcgaggt gctcgccggc cgcccgcaca ccgccctgtc cgtggaggcc 99900

ccgggccgcc cgggcctcgc ccatctgctc ggcaccctcg ccgcgctcgc cgtacgcggc 99960

acacccgtac ggctggacga gctgaccgtc gcccgcgacg agccggtgtc cgcgcccccc 100020

ggatggaccg tcgacggcca cctggtccgc accgcggacg gcacgcccgt ccccggcgga 100080

ctccagccgg ccggcccgcc cgtgaccctc accggcggcg gcgtgcccga cagccgcgac 100140

gagaccgtca tcgaattcct gcgcggcacc cagcggatcg tggccgcaca gcaggaggtc 100200

atgctccgct acctcggcac gcaacccccg gccccggagc cggcccccgc gcacgaccca 100260

ccggaatccg ccgcgaccgc cgcgcccacc gtcctggacc tggtggccgc gcgcaccggc 100320

tacccgccgg ccatgctgga cccggacctg gacctggaag ccgccctcgg cgtcgactcg 100380

ctcaagcgca cggagatcgc ctccgccctg gtccgcggca ctccggccgc ggacgccgtc 100440

ggcgaactcg ccgacctgcg caccatccgg gcgatgaccg actggctcgc cacccgcacc 100500

ggccccgacg gccccggccc cgacggcccc ggccgccaga cggccgtgcc ggagcccgaa 100560

accatgcccg ccggcgcctc ggacaccgtg cgccacgtcg tcgaacccgt gcccgaggaa 100620

ccgcccgccg gtccgctgcc gaccctcacc cgggccgccg tcagcggcgg cggtgccgtc 100680

ggcgacgtgc tggccacgct gctcaaggaa cgcggcaccg aggtcgtcag cgaccccgcc 100740

ggctgcgacg ccctcctgct gctcgatgcc ctcgacggcg gcgactacac cctgcccggc 100800

cggttcaccg acataagggc ggccgtcctg ggcggtttac gcaccctgct gctggccacc 100860

tgccacgacg cgggacccgc cggcagcgga gtgcacggcc tcgcccgcgc cctctcccgc 100920

gaacaccccg gcctcgccgt gaccgccgtc gacctgcccg ccggacagcc ggccgaggag 100980

gcggcgcgca ccctgctcgc cgaactcggc ggcagccggc cgtccgtgac ccacaccgac 101040

ggccgccggg ccgtctggcg cacccggccg gccccgctgc ccgccgccga cgtcaccgcc 101100

ggcgacctcg gcctggaccg cgactccgtg gtgctcctca ccggcggcgc gcgcggcatc 101160

accgcccgcg tcgcccgcgc cctggcgacc ctcaccggct gccacctcga actcgtcggc 101220

cgcagcgaac ccgtcaccgg gacggtcctc accgacgccg atctgcggac ccgcctgatc 101280

gccgagggcg ggcgcgaccc ggccggcatc gaacgcgcgg tgcgtgccca cgccgccggc 101340

cggcaggtcc ggcagtgcct ggacgacctc gccggcccgg ccgcctccgt gcgctaccac 101400

cgcgccgacg tacgcgaccc cgagcggctg ggcgccgtcc tggacgacgt gtacgcccgg 101460

cacggccggc tggacaccgt cgtccacgcg gccggccagg tccgcgaccg gctgctgcgg 101520

gacaagagcc ccgacgagtt cgccgaggtg tacgacacga aggtcgcggg cgcccgggcg 101580

ctggccgccc ggctgcggcc gggcctgcgc cacctggtgc tcttcggcag catcgccggg 101640

gtgaccggca accgcggcca gaccgactac gccgccgcca acgacgccct ggacaccatg 101700

gcccggcagt ggtccggccg ggtcgccgac cgggtcctcg ccctggactg gggcccctgg 101760

gcggccgacg cgggcggcat ggtgaccgcc gaactggaac gggcctacgc ccgcaacggc 101820

atcggcctga tcgacccgga cgacggcgta cgggccttcc tgcgcgaact ggccttcggc 101880

cgcgacccgc aggtgctgct caccgtcggg gaccccgccg ggttcgggag cgcccttgac 101940

tgacatactc ctcgccgccg cggccggccc cggagccctg ggcgccgccc tgcgttcggg 102000

ccggcgcggc ggcgccggac cctgccgggt cgccgtgctc gaccccacgg ccgagcggat 102060

cgcacaggcc ctcgccctga tcgaccgggg cgaaccgtgg cacggcggca acgacatccg 102120

gttcgcgccc cggcccgggc cggccggccg caccgtcctg ctcttccccg ggctcgacgc 102180

ccgtaccggc ccgccacccg acgacgtcct gcgctggctg ggccgccccg ccggaccggc 102240

gaccggcgcc gccaccctcg ccgagcgcac ccacgccacc ctgcggctca accgggtgct 102300

ccacgcggcg ctgctgcggg ccgggatcgt cccggacgcc atggcggggc acagcgcggg 102360

ggagtggagc gccctgttcg cggcgggact cttccccgag acggcgttcg acgagttcgc 102420

ccggaccatg ggcgccgccc cggtccccga cgtggtcttc gccgccgtcg gcctgcccgc 102480

cgaagcggcg gcccggctca ccgagggaga gccgcgggtc gtgctgtccc acgacaacgg 102540

gcccgaccag agcgtgctgt gcggcgacga ggacgccgtc acacgctgcc tggaccggat 102600

cgccgacccc gaggtgccgc gcagagtgct gccgttccgg tcggggttcc actcgccgat 102660

gctcgccccg tacctgccgg gcttctcggc cgtgttcgac cgggtgccgc tcgcccgccc 102720

cgccgtcccc gtctggtccg cgaccaccgt cgccccctac ccggacgacg agaccgagct 102780

gcgcgccctg ctcggccggc acctggtgga acccgtccgg ttccgtgccg tgatcgagcg 102840

gctgtacgag cagggcgccc gcgtcttcgt gcaggccggc tgcggcagcc tcacccgctt 102900

cgtcacggcc accctcgccg gccggcccca cctcacggtc agcgcgggct cggcgacccg 102960

gccccaactc gaccagctgc gctgggccgc cgccgcgctg tggacggcgg gtcacacccc 103020

ggacctggcc gccctcggcc tcaccgggcc cgcggcggac ccggtgccga gcacccgcgc 103080

cgtgaccggc gcgcacccga gaaccggcgc gtacccgcgg acccacccgg tggccggcgc 103140

ctacgaggcc gccgtcgccg cggtgagcgc ctccgtggag agcgtctacc ggacctggaa 103200

ggagcggaca tgagcagccg gcgacacgcg gtcgtgaccg gatcgtcccg cggcatcggc 103260

gcggccgtcg ccgcccggct ggcccgggcg ggctgggcgg tcaccggctg ggaccggaca 103320

cccggcgaca ccaccggcct cgccgactgg cgcgaggtgg acgtctccga cgccgagtcc 103380

gtccaggccg ccgcccgcga ggtgaccgcg accgacctcc tcgtcaacag cgcgggcgtc 103440

ggcgccatcg ccccgtccgc ggagctgaag ccccgcacct gggaacgggt cgtcggcgtc 103500

aacctcagcg gcacgttcta ctgcgcccag gcactgttcc ccgccctgtc cgcgcgccgc 103560

ggactggtgg tgaacctcgc ctccgtcatg gcacaccggg ccgtccccgg ccgcgccgcc 103620

tactgcgcgt ccaaggccgg cgtggtcatg ctcaccgagg cgctcggcgt cgaatgggcg 103680

gagcacggcg tccgcgtggt ggccgtcagc cccgcctacg tccgcacccc cctcgtcgcg 103740

gagggcttcg ccaacggcaa cctggacgag tccgccatcg ccgcccgcac cccgctgggc 103800

cggctcgccg agcccgagga gatcgccgac ctcgtcctgt ccctcaccgg cgacgagttc 103860

gcctatctga ccgccaccac cctgcgcttc gacggcggct ggaccgccga cggcgtgttc 103920

ccccgctgaa ccggcccgga cctcgctgaa ccggcccgga ccccgctgaa caggcccacg 103980

gacaaggaga accagacccg atgcccgtca cggacttcgg cgtcctcgcg ctcgcccacg 104040

ccctcggcga tccgcgcgac gtcgccgcca ccgcagccga ccacgtcgac gaccccgacc 104100

gcgtcctgct ctggggctac cgcggctacc accgcgcccc gcagggcacc acctccaccg 104160

cgctcgccgc ccgcgccgcc gagaaggccc tcgccaaggc gggcgtcgac ccccgcgacc 104220

tcgacctcgt cgtggtcgcc gacagcagcg tccccgagta cctgctgtgg gacacctccg 104280

ccgtcgtcgc ccgcgcgatc ggcgccggca cggcgccgac cctgctgctc acccagggct 104340

gcgcgtccgc ggtcaccgcc ttccagcaga tcgccggcat cttcgcgacc cgccccgacg 104400

ccgagacggt cctgctggtc gccgtcaacc aggtcagcga ggcgcacacc aaccgcatgc 104460

gcttcaacac cctgctcggc agcgacggcg ccgcggcggc cgtgctgcgc cgaggtcacg 104520

accggctccg ctggctggcc accgagcagc tcaccgaccc cacgtacgcc gacttctacc 104580

gggtcgagta cggtggcgcc gccgtcccgc acgcccccga gggcgccggc aacctggacg 104640

tcgacccgct gtccctggtg taccggcact tccgccggga gcccgagcac ctggcgaagt 104700

tcgtccgcac cctcaacagc cgcgtccgca ccgtgttcga ggacgcgtgc gacggggccg 104760

gagtcgaccc cggccaggtg aagcggttcc tgttcctcaa cgacaaccag gactccctcg 104820

ccgacgtcgc caaggccgtc ggcgtgcccc tggaccgcac caacgccgaa ctggccaagg 104880

acctcggtca ctgcggcggc gccgaccagc tgatctgcct ggacaccctg ctcgaacgcg 104940

gtgagctcgc cgagggcgac gtggtcgcgc tcgcgggcct gtccatcggc atgcactggt 105000

actgcaccct gctcaccgtc tgacccggca ccctcaccga gccctcaccg acagcccgcg 105060

acaccgagag aaacagagag aagaacgacc atggctgagc tgaccatcca ggacctgatc 105120

gacatcttca accgcagcat cggcgaccag gacccggtcg agcccgaggg cgacccgtcg 105180

gacgtcacgt tcgccgccct cggcttcgac tcgctcacca cgctgaacgc cgtacgccgg 105240

atcgagcgca agcacggcgt cgaactcggc gagaacgtga tcagcgaggc ccgtacgccg 105300

cgccagctgc tcgaccgcgt caacgccgcg ctcgccgcgg cctgaagcac gaaaagagac 105360

acgacatgtg cggaatcacc ggatgggtgg cctacggcga cgacctcgcg cggcaccggt 105420

ccgtcgtcca ggcgatgacc gacaccatga tctgccgcgg cccggacgcc gagggcgtct 105480

ggatcgaggg acccgtcgcg ctcggccacc gccggctgtc catcatcgac ccggcgggcg 105540

gcgctcagcc catggtcgcc acacgcgacg gccggaccct ggcggccctg accttctgcg 105600

gagagatcta caacttccgg gagctgcggg ccgaactggc cgccctcggg cacacgttcc 105660

gcaccgacag cgacaccgag gtcgtcctca acgcctacct ccagtggggc gccgacttcg 105720

ccgcccggct caacggcatg ttcgccatcg gcctgtggga cgcgaccacc cgcgaactgc 105780

tgctcgtccg cgacccgatc ggggtcaagc cgctctacta cgcccgcacc tccgacggcg 105840

tcgtgttcgg ctcggaaccg aaggccgtcc tggcccaccg cggcgtgccg cgccgcgtcg 105900

acgcccaggg cctcgccgag atcctggaca tggtccgcac cccggaggtc acgccgttca 105960

ccgacctgtc cgaggtccgc cccggacagg tggtgcgggt gaccgagggc ggcctgaccc 106020

ggacccggta ctggcagctc accgctcgcg agcacaccga cgacctggac accacgatcg 106080

ctaccgtccg ggagctgctg gaggacatcg tctcccgcca gctcatcgcg gacgtcccgg 106140

tcgccaccct gctctccggc ggcctcgact cctcggccat caccgcgctg gcccagcgca 106200

cgctgaccgc cgagggacgc ggcccggtcc gctcgtactc cgtggacttc cagggcgcgg 106260

ccgacgggtt cgagccggac gcggtccgcg gcaccgccga cgcgccgtac gtgcgggact 106320

gcgccgccca tgtgggcgcc gaccacagcg aggtcctgct cgacagcacc gagctggccg 106380

acccggcggt ccgggcggcg atcctgggcg ccaccgacct cccgccggcg tactggggcg 106440

acctgtggcc gtcgctctac ctgctgtgcc gcgagatccg caagcgggcg accgtagtgc 106500

tgtccggcga gtccgccgac gagctgttcg gcggctaccg ctggtaccag cgccccgagg 106560

cggtcgacgc gccgaccttc ccctggctca cccccggctc ggcgcgcatc ttcggcggca 106620

gctcgctgat cgaccagggc ctgctggaga agctggacct ggagggctac cgccgggacc 106680

gctacgccga ggccctggcc gaagtgcccg tgctggccgg ggagtccgcg gtcgaccggc 106740

gcatgcgcga ggtcacctac ctcaacatca cccggttcat gcaggccgtg ctggaccgca 106800

aggaccgcat gagcatggcc gtcggcctgg aggtccgggt gccgttctgc gaccaccgcc 106860

tgatggacta cgtgttcaac gtgccgtggg cgatgaagtc gttcgacggc agggagaaga 106920

gcctgctgcg cgccgccgtc cgcgacgtgc tgccgaactc ggtcctggag cggaccaaga 106980

ccccgttccc cgccacccag gacacccggt acgagcaggc gctgcgcgcc gagctgcgcg 107040

aggtgctggc tgacccggac gcgccggtgc ggccgttgct caacaccgcc cgggtcaacc 107100

gcgtcctcgg ccgtgagctg gacgacttca gcctgccgca cgaccggggc ggcatcgaga 107160

tggcgctgtg gctcaaccgg tggctcgcct cctacgaggt caccgtgacc gtctgagccc 107220

ctggagcgac gcccgcacgg gccgggcgtc gctccgctcc gacgcacggt ccgcgtcaca 107280

tcgccggccg aaacacctcc cggcctcaac cacgctccga acaagtccat ttcgcacaca 107340

gcatgttcca gcaaggggga tttacatgcc cgaccggtcc agcgcagagc accaattgtg 107400

gctgcgcggt ttccaccagc cgaagccggg cgcgccgcgg ctgatctgcc tgccgcacgc 107460

gggaggctcg gcgagcttct acttcccggt ctcccaggcg ctgtcgcccg cggtggacgt 107520

gctggcggtg cagtaccccg gccgccagga ccgccggcag gagtccccgg cagcctccgt 107580

ccaggaactc gcggagggcg tgttccgcgc gttggacgat caggaggaca cgcccctggc 107640

cctgttcggc cacagcatgg gcgccatggt cggcttcgag ctggcccggc tcctggaggc 107700

cgccgggcgg ccacccgcgg tgctcttcat ctccggccgc cgaggcccgt ccatcgtgtg 107760

gacggaaacc gtccacacga tggacgacga acggctgatc gcggaggtcg ccaagctcga 107820

aggcaccgac gcggccctgc tccaggacga ggaggtactg cggatgatcc tgcccgcgct 107880

gcgggccgac taccgtgcgg tggagaccta ccagcgcacc cccggacccc ggctgagttg 107940

ccccttcgtc gtgatgacgg gcgacgccga cccccgggtc acccccgacg aggcgcggac 108000

ctgggcagag gagacggacg gggccttcga actcgaggtg taccccggcg cgcacttcta 108060

cctggtggcg cagcagcagg ccgtgctggc ccggatcgag gccacgatgc ggcggctcgg 108120

ttccaccacc ggagcggtgg tctgagcctg tgaacgagat actcagcgcc gtcctgtcgg 108180

ccgaggcgac cgccgcggac ttcgcggccc tgccgctccc cgagtcctat cgcgccatca 108240

ccgtgcacaa ggacgagacg gagatgttca ccggtgtcgg cccggccgac aaggacccgc 108300

gcaaatcact gcatctggac gaggtgccgg tgcccgaact gggcccgggc gaagccctgg 108360

tggcggtgat ggcctcctcg gtcaactaca actccgtgtg gtcctccatc ttcgagccgc 108420

tgccgacctt cgctttcctg gagcgctacg gccggcgcgg cgaactggcg aagcggcacg 108480

acctgccgta ccacgtgatc ggctccgacc tggctggcgt ggtgctgcgg accggccccg 108540

gggtgcaggc atggaagccc ggtgacgagg tcgtggcgca ctgtctgagc gtggagctgg 108600

agtcgagcga cggccacgac gacaccatga tggaccccga gcagcgcatc tggggcttcg 108660

agaccaactt cggcggtctg gcggagctcg cgttggtgaa gtccaaccag ctgatgccga 108720

aaccccggca cttgacctgg gaggaggccg ccgcgccggg actcgtcaac tccaccgcct 108780

accgacaact ggtgtcgcgc aacggcgcgc agatgaagca gggcgacaac gtgctgatct 108840

ggggcgcgag cggcggcctg ggttcgtacg ccacacagct ggcgctggca ggcggtgcca 108900

acccggtctg tgtggtgtcc acgccgcaga aggcggcgat ctgccgctcc atgggcgctg 108960

aggcggtcat cgaccgtgat gccgaggact accggttctg gcgggacggg aacacccagg 109020

acccccggga atggaagcgt ttcggcaagc gcatccgcga gctgaccggc ggtgaggacg 109080

tggacatcgt cttcgagcac ccgggccgcg agaccttcgg cgcgtccgtg ttcgttgcgc 109140

ggaagggcgg cacgatcgtc acgtgcgcat ccacctcggg ctatgagcat cagtacgaca 109200

atcggtatct gtggatgtcg ctgaagagga tcatcggttc ccacttcgcg aactaccgtg 109260

aggcctggga ggccaaccgg ctcgtcgcga agggcaggat ccaccccacg ctctcgaagg 109320

tctacccctt ggaggagacc ggacaggccg ccttcgacgt gtacggcaac gtgcaccacg 109380

gcaaggtggg ggtcctgggc ctggcgccca cggaaggcct gggtgtgcgg gacgaggaga 109440

tgcgcgccag gcacgtagga gccatcaacc gcttccggcc gtccgcggag ccgcagacag 109500

agccggtctg agggctcgac ccgcaaaaga ccgcacacga tgcccacttc tgcaacagag 109560

cagctttgaa aggcaggacg tcatgagcaa cccgttcgac aaggaagacg gcagcttctt 109620

cgtggtggtg aacatggagg ggcagcactc cctctggccg gccttcgccg agatccctgc 109680

gggctggtcg atcgtccacg gcgagaccga caaggcggcc tgcctggagt acgtcgccgc 109740

tcactggacc gacatgaggc ctcgttcgct gaccgatgcc gcgccgaccc cagagtagaa 109800

ctccccttcg gaaacagtcc gttgacaaca aagtgttcaa atgccagggt tgccgcatgt 109860

catcgattcc gccgcagcca gccgatgcct tcaccgcccc gagcgcgcac gaagccagag 109920

tgcagcgtgc gtacacctct cttttccgca tcgccgaacg gcacgcggcc accgacgggc 109980

agcgtcgtcg gcaggcccag tctcacatga tcagccccta tgaggcggtg aggcttgtgt 110040

cgttcctcct cagcggggcg gcgcagctcg agggagagga accggaggtg gatcgcgcgg 110100

acatcacggc cgcgttgagt cttctcccgc tcgctcgtgc cgagatggac gaagtggagg 110160

ccggaattct caagatggcg catggtcgtg ggatgacgtg gtcggagatc gcgttcggcc 110220

ttggcctcgc aaccccgcag gcggcgaggc agcgccatga gcgcctggcc agccgcatgg 110280

aatccggcgc cgaaaaaaag gccgacggat aggagctcgc catcggcatg gcggcagccg 110340

accgccgcca tgccgatgcg tcatatccgt cagccgagaa tcctggcgaa tccgatgtgg 110400

acgcaccggg ccgacggccg cgagggcgtg cccgggctcc gaacggggcg tcggcgccgg 110460

ggaagtcgtc ggccttgctc gacgtgctca cagccgcgtg aacccgacgg ccacgacgcc 110520

gatgcccacc aggaggcaga ggagccacaa ggcgacctgg gagcccgtgc cgctggcata 110580

gcggcgggcg gtcgcgcgcc cgagggcgcc tatcgcgcat ccgaagacga tgaggaccac 110640

acccacggcc acggcgtcat cccttcctct tcttgttcca tatcccgtac gcgaacatgg 110700

cgacgatccc caccaggatc agaatcgcga cgacggaccc cgcggtgccg gccgggatcc 110760

agcttccacg accgtcagcg gccagcagca tcgctgcact ccttactcag tacgagcact 110820

gggtgtgggt tggcccgcga gcccccaagg gcaccccggg ccggccgatt gtctcaggat 110880

caggattcgg acgccgcgtc ctccacctga ctacccgcga aatcggcgaa gatgccgccc 110940

ccggcaccgg ccaccgcgcg gtggagccac tgtccgcagc ccctgacccg ctcagccact 111000

caccagtgcc tgggcgtgtc gctcagcgac ggacttgggc gccaaccggc cggtggggga 111060

ggtgggctac tcggcccgcg gcgcgggccg tacgggcgtc ttgcacccga tgactcgctc 111120

cgatagccaa ccccgcccca agagcagcgg cccgctccgg gccgcgtcca cgtcgtccag 111180

gacggcgacg ctcactccgg ctcaccgcca cggcggccgg ggagatctga ctacgtccgg 111240

acacaccacg ggctccgagg acgggcagcg gtgctcctcc ttcgtcagat gtacggctcc 111300

gcagcggtgg cacctctgga ggtcatcgaa gccggtcgcg caggcgaagc agaagtccac 111360

cggcggttca gccgcttccg ggtccgttcc ggggtgcagc tggacagcga gccgtcctcc 111420

aaggccgtac gggtggccgc tccggggtcg gtgaacacca ggctccagtg ctccatgagc 111480

agccgggctt tgggaaggac ctcggccccg gcggccaggt gcagcaccgc gtacttcaga 111540

tcccgggcgc tgcccgcccc tttctcgcgc tgcagaagct cgacgacgct gacaaggtag 111600

tcgagaccgt tggcgatggg cgggaagtgc gggtctccgg cgaaccgggt ttcagcctcc 111660

gaggctctgg cgaatgccgg ccagttgggg gttctgccag gccgggaggt agtcggggtg 111720

gtctcgccac tgctcgcctg caccggtcag caggtgccaa gcccgtgtca cgctgtcggt 111780

gtcgccttgg gcgaggaggt acgagacgga ctcggcggtg cccgcgaggc cgtacttgct 111840

ggcattggag acgcggaagt accgggcctg agcctcctcc tccagggtgt cgtcggcgag 111900

cgcggccgcg tgctcctcgt cgatgcgccg gctgatgaag tcgtacagct ctcggacatc 111960

cgcagcggtg ggcacgttcg tggtcatggg gtgcgctcct cctctgaggg cggctcaggc 112020

cggcagcctc atgtaaggga atttatacga gggtgatgaa ggcttggctg cgtaggttag 112080

accgaagagc acttgtcggt gagagctggg aacagcaggc tgccgccggg cctccgaatg 112140

ttgacgagaa gtagcagcgg tcccatgcca cggaaacgtg ctgtcgtcaa cgtgttgacg 112200

tccgtatcct ttggcgggtg agtctgatcg aggtgggtcc agggcaggtc gagctcgtcg 112260

tgcgggggcc gggggtgctg gcgacttctg tccggttgtt cgactggtcg cgtgcggatg 112320

agtacgagac cgtcttcgcg gtggaggcca ttgctgatgg tgtgcgtgca cggctcgaga 112380

acgtgaccgt caccgtctgg gatgacatga gcgcgttctt cgacggcctt gcacgtgatt 112440

tccgtggttg ggatggggag cgggtttgga tcaacaacca cctggtcgtg acggcgactt 112500

tcggctcggg tggtcatgtg tatgtggact ggacgcttca gtccggcttt tttcccggtg 112560

actggaagtg cacggtgacg accgtgatcg aagccggtga agggatgacg gctgtagctg 112620

cggacctgcg ggaatttctg cgccaggagt aggtccccac gtcatccggc aggcacgccc 112680

gtccggtcca gccgacggct tactggtcag cgcgggcgcg ggtggtggtg agacggtagg 112740

agtcgtgccg gtatcgatga tgttgccgcc ccggtccgag cggtcacgcg gacccaagcc 112800

ggtccccgac cggctgtgct tgcagggcat cctgttcgtg ctccacaccg ggatcccgtg 112860

gcagcagctt ccgctggagc tgggtttcgg ctccgacggc atcccgccgg tcgccggccg 112920

agtcggccac ccccgcaagc gccccgacgc gctacttggc gacaagggct acgacagcaa 112980

cgcggaccgc cgagagttgc gtcggcgcgg gatcctgccg gtcatctcct cccgcaaggt 113040

gcagcccgac atccatggcc ccgacatcca tggcctgggc aggatccgct acgtggtgga 113100

gcagatcttc gtcctgctcc accagttcat gcgcctcgct gttcggtggg aacgccgttt 113160

ggctctgcac gacgccttcg tctcggtcgg gtgctcgctc atctgctgga ggaggctcaa 113220

gaggccgctc acctcgcagg cccggcgctc aagcccgtgg cccgtcgggc attctcacgc 113280

cgccaccggg cagtgatctg atgaagtagc tgccccgcag accgatgacc cagccgaagg 113340

catcggagga aacgggcacg tccagacgcc atccgttggc caagcggact cgcaggctgc 113400

ctgaatcggc tgcgtgaacc tcggcgatca cctgatcgag cagctgcggc agccggtcaa 113460

ggttggtccg tgtggatgcg tggacggaca cggcttcgtg gtccgtcgtc agttcggcgg 113520

gactgtgaac ctcgatccgg ttctcaccgg tgtgaagccg caactcgttg ccgatgatca 113580

gctctccgat gccgctccca cgcatcgaga ggatgtccca cccgtcgtcc acacggttgg 113640

ttccgttgtc catgccgcaa gactacggac cccggctacc acgctgccgc acaagatcgt 113700

gtgacgaact ctaacgagct ggtgtgtgac tccggttgaa gggtgatcgc gcgaaggtgg 113760

gttgatccag tccgtgttga tcatggtcgt cgaccgggat atcctcgcgc atcagctgtt 113820

caccggcgtc tcccggcagc atctggcttg ctcggtcgag gagttggccg aaccgtggca 113880

ggcgatcaca ccgcccagaa agccgaacaa gctcgctttg ccgcaggtac acgcagccag 113940

agaagaagcc cggcaccgac actcatcgaa gcgcatcacc gtcgagcacg cgctcaccga 114000

ccacaaacgc tcgaagcagc tgacccgctg gacgcaccgg tgagagggcc ggcccgacac 114060

ctaccgggcc atcgccggcc tcgtctccga ccgcaccgcc aacgcgtgac catgaccccg 114120

atcaggcgaa cacgcctcac ggtcaagagc cggccgctcg ggcaggtgag gccggtctcc 114180

tcctgcgtct ctcgtgtcgc ggcggtgcgg ggggactcgt ggtggtcgag gttcccgccg 114240

gggaactgcc agatctgcct gccagtgcgg cggccgcgca cagcgcgtcg ggcacggtga 114300

gcgtgtaccc ggcctggccg gcggtccgga tcagaacgcc gctgggatcg ctgccgccgc 114360

tgtgagcgtg cggacggacg tcatcggggg agtgaccgga gcgaccgtct ggctcttgtc 114420

gctggtgagg catttctgga aggaatccgg ttgcctcgct gttctcggca gtgccaggtg 114480

gccccgccgt cgctgacgac cgcggcttca cggcccgtcc tgcgggtgga gtcggcggcg 114540

acctgcaaag ccgtcgcgca gtcggtgccc tgcgcggccg acaccgccag gccggtctcg 114600

ccgcggtccg tgccgcggtg cccggcactt cgcacgcgga cacggctgca gggccggaga 114660

cgaccgtcgc cgatacggcc atcaggcctg ccagccacta cccaacttcc ccgagtacta 114720

cctgagccga ggtcgcatcg gcgtacgcac aggcacgctg tccgcccctc agtggacggc 114780

cctcaacgcc ctgctcgccg cagccctgtg ctcgtcgccg gactcggggt acgacaagat 114840

ccaacagcac ctcgacgcgg acgatcacct tcgccggcac ggcggtggcg acgcgtacgg 114900

gcgcggcaac ttcttcgtcg ccttcctcag cacacccgcc gacaccggga tctgggagct 114960

gcagttcggc ggccatcacc tcgcggtcgc ccacacctac gccgacggac gcctgatcgg 115020

tgccacaccc gcgttccgcg gcatcgagcc gttccggtcc ttcacccggc acggcatccc 115080

gaccagcccg aacgagccga acagcaggcc ctctccgccc tgctccagtc cctcgacgcc 115140

tcgcaggcgg cggccggact cagggagcgg cacgacgacc ttctgctcgg cccgcaccgg 115200

gactggaagt tcccccggaa gtccgaaggc atcgccgccc acgatctgac agccgcgcaa 115260

cgtgacctgc tcgtcgcggc catcgcccgt tacgtcggcg acttgcccga agcggacgcc 115320

cgcaggtttc tcgaccgcta ccgggccgaa cttcccgaca cccacgtcgg cttcaccgga 115380

tcgacactgc tgaccgaccc cggtgactac gtccgcatcg acggtccgtc ggtgtggatc 115440

gagttctcca tgcacgacgg catcgtcctg gccgatccgc acccccacgc cgtctggcgc 115500

gacaagcaca ccgactacgg cggactcaag ccctgacgcg gtcccgccgc cccgtatccg 115560

gggcccgacc acgccccttc cccagctcac accgcgcggc ggtcgcgcgc ggggctcacc 115620

gctggacggc gcgcagtccg tccaggagca gccgcagtcc gaactcgaag tgtgccgcga 115680

agtccgtgct ggtgagcact ggcagggtgg cggcgaggtg tggatatgtc cccgccgcca 115740

cggcctcgtg cagggctccc gcatcggtgg gctccccgtc ccccggcccg agggcggcct 115800

gttcctccag ggtgtggccg acggtgaagt tggtcaccga gtagagcgcc cgcgccgctt 115860

cgccgtcacc gaaacccgcc gcacgcagca caccgacgag gctgtcggcg aagcccaggg 115920

tgttcggacc ggtcgagtgc gtaccggtga agacgcgtgc gccgtcgcga tgggcgagca 115980

gggccgtacg cagcgcgcgg gcgagccgcg ccgctctctc gctccagtcg ccgtcccccg 116040

ccggcccggc ggcgtcggcg acgccgccga ccatccgctc cgccatcgcg gtgagcaggt 116100

cctgtttggt cgcgaagtag cggtacagcg caccggcctg gacgcccaat gcgtcggcga 116160

gccggcgcat ggtcaacgcg tcgagaccgg actcgttcag caggtcgagg gcggtctgga 116220

cggtgcggac ctggctgatc cgggcgggcc ggcctctggt cgggcttgac ggggtgctca 116280

tggacctcac tatactgaac accgttcacg tgaacgctgt tcactaaatg tgggaggaca 116340

tctccgatga acaccgatgt gatcgtcata ggcgccggcc cgaccgggct gatgctcgcc 116400

tgcgagctgg cactggcggg cgtacggacc cgcgtcctgg agcgccgcgc cgaaccgcag 116460

cgcaattcgc gggcgttgag cctgcacccg cgcagcgtgg aactgatgga ccagcgagga 116520

ctgctcgacc ggttcctccc gctcggccgg accgtgccgg gctggcactt cgcagggctg 116580

cgcacccggc tggacttcgg cgccctcgac tcccggcacg gctacaccct cttcctggct 116640

caggagcgca ccgaggcgat cctggaggag cgggcacacg agctgggggc ggagatcagc 116700

cggggatacg agaccctcgg tgtgagccag gacggcggcg gggtcgaggt gcaggtgcgc 116760

gtccccggcg gcggcaccga gaccgtccgc ggcctctacg cggtcgggtg cgacggaggg 116820

cgcagcgtcg tgcggcaggc cgcagggatc gacttccccg gcaccgacga gaccctgacc 116880

ggggtgctcg gggacttcgc ggtcgtcgat ccgcggcccg gcgccctcga tgcggcacga 116940

gcccgcggcg tgatcatcgc gccgctggag ggagggctca cccggttcgt ctacctggat 117000

cccgagcgga tacgggtgcc gtcccgggag cccgtgaccc tggaggagtt ccgtacgtcg 117060

ctcacccgga tcaccggctc cgactgcggg atcgccgagc cccgttggct gtcgcgtttc 117120

ggcaatgcca ctcggctcgc ggagagttac cgtgcgggac gcgtcctgct ggcgggcgac 117180

gccgcccaca tccacttccc cgcggcgggc caggggctca acaccgggct ccaggacgcc 117240

atgaacctgg ggtggaagct ggtcgccgcg gtgggcggct gggcgccgcc cgggctgctg 117300

gacagctacg acggggaacg caggccggtc ggccggtcgg tgaccgagaa caccgaggtg 117360

cagactctgc tggcggagct gacgctggtc gcgcagtacc agcgtccggc cgcggccctg 117420

cgcgagctcc tcgaccaact gctgggcatg acggaggtca accgccggct cgccgaccag 117480

gtctccgcgc tcggcaccca ctatccgccg gcggactcgg acgctgatcc gctggtgggg 117540

cggcgtatgc ccgacatcgg cctgaccgcc gccggttcgg cggcgacacg ggtgtacgaa 117600

ctgctcaccc ggggccgctt cgtcctgctc gccctggccg gagacccggc ctctcgcgag 117660

gccgtcgacg cgggctgggg ctcgcgggtc tcggccgtca ccgtggacaa gtgcgacgag 117720

cattcggacc tcgacggggt ggccgaggtt ctcgtacgcc ccgacggcca cgtcgcgtgg 117780

gccacccgta ccgccgacgc cgacccgcgc ggcgacgagc gggtccaggc gctgaccgct 117840

tgggcgggca cgccctcacc gaggagctga tcatgcccgt gaaccgcgtc agtccaccgt 117900

ccgtgcacaa cccgtccgac cagatcagcc aggtcgtcac cgtcgacgga acccggctcg 117960

cgcatctctc cgggcaggtc gcctgggatg cggaaggatg gcccgtgggg ctcggcgacc 118020

acgaggccca ggcggcacag atcgcacgca acctcgacgc cgcactcgcc gcggtcggcg 118080

ccacccgcga cgacatcatc gaagagaccg tctacgtagt cgactacacc ccgaaactgt 118140

tatccgcgat cttcgggccg ctacgggccg gtgtctcagc agcacctgcg agcacgctgg 118200

tgggcgtgcc cgcgctcttc gcaccggaat acctcctgga ggtccaggtg gtcgccgcgc 118260

tccccgtacc agggggccat gaccgcgcca caccgaccga cgacaccgcg gcgaacgagc 118320

gtgtcagggc acgggagccc cgcaggatcc gtgaaacacc ccccacgggc ga 118372

<210>2

<211>146

<212>PRT

<213>Streptomyces flaveolus

<400>1

Met Trp Arg Pro Ala Pro Pro Ser Arg Met Cys Ser Arg Ser Ala Ser

1 5 10 15

Phe Val Ala Ile Arg Thr Phe Ser Arg Ser Ser Ala Ser Ser Thr Ala

20 25 30

Arg Asp Val Arg Tyr Thr Ala Ala Ala Ser Ser Val Val Arg Leu Arg

35 40 45

Glu Ser Glu Cys Arg Arg Gly Asn Asp Thr Leu Ser Ser Ala Pro Tyr

50 55 60

Arg Asp His Ser Ala Pro Pro Arg His Pro Tyr Arg Ser Ala Ala Gln

65 70 75 80

Pro Val Gln Leu His Gln Ala Ala Arg Arg Ala Glu Asn Pro Ala Pro

85 90 95

Pro Ala Ala Arg Arg Ala Ala Ser Arg Thr Arg Arg Pro Lys Thr Pro

100 105 110

Ile Arg Arg Leu Pro His Arg Ser His Arg Thr Gly Arg Thr Ala Arg

115 120 125

Pro Gln Gln Gln Arg Leu Leu Ser Pro Val Arg Arg Arg Ala Arg Gln

130 135 140

Ser Pro

145

<210>3

<211>155

<212>PRT

<213>Streptomyces flaveolus

<400>1

Met Arg Gly Tyr Phe Met Ser Lys Arg Leu Val Val Ser Ser Leu Ala

1 5 10 15

Val Ala Ala Ala Val Val Ala Gly Thr Val Val Phe Val Ser Ser Ala

20 25 30

Asp Ala Ala Val Pro Ala Lys Pro Glu Ile Ser Lys Ala Thr Ala His

35 40 45

Tyr Thr Ser Thr Ala Gly Gly Ser Ala Ser Leu Thr Phe Ser Ala Thr

50 55 60

Val Ala Asp Asn Ser Gly Ile Lys Ser Leu Arg Val Leu Ala Trp Pro

65 70 75 80

Ala Ser Ser Gly Leu Ala Pro Thr Ala Gly Glu Met Arg Asp Val Glu

85 90 95

Glu Ala Thr Cys Lys Ala Thr Ser Ala Thr Ala Ser Val Cys Thr Tyr

100 105 110

Thr Val Lys Ser Ser Ala Lys Glu Ala Ala Ala Leu Pro Lys Gly Val

115 120 125

Trp His Val Ser Val Leu Ala Thr Ala Lys Asp His Asp Thr Thr Phe

130 135 140

Ala Pro Gln Gly Ala Thr Phe Thr Val Lys His

145 150 155

<210>4

<211>276

<212>PRT

<213>Streptomyces flaveolus

<400>1

Val Glu Ile Gly Ser Gly Ala Pro Glu Leu Thr Ala Ser Ser Val Tyr

1 5 10 15

Gln Gln Arg Arg Asp Gln Ile Ala Ala Ser Ala Ala Ala Tyr Val Pro

20 25 30

Gly Glu Pro Ile Pro Glu Val Glu Tyr Thr Asp Ala Glu His Ala Leu

35 40 45

Trp Arg Leu Val Ser Lys Arg Leu Ala Asp Arg His Arg His Met Ala

50 55 60

Ala Pro Glu Phe Val Glu Ala Ala Glu Arg Leu Glu Val Gly Gly Asp

65 70 75 80

Gly Val Pro Gln Leu Arg Glu Val Ser Asp Arg Leu Asp Gln Leu Thr

85 90 95

Gly Phe Arg Leu Arg Pro Ala Ser Gly Val Val Pro Phe Ala Leu Phe

100 105 110

Cys Gly Ser Leu Ala Asp Gly Tyr Phe His Ser Thr Gln Tyr Leu Arg

115 120 125

Asp Ser Ala Thr Pro Phe Tyr Ser Thr Glu Pro Asp Ile Leu His Glu

130 135 140

Val Ile Gly His Gly Ser Ala Leu Ala Asp Asp Arg Phe Ala Asn Leu

145 150 155 160

Tyr Arg Leu Ala Gly Glu Ala Val Arg Arg Val Glu Ser Glu Asp Ala

165 170 175

Val Gln Phe Val Ala Lys Thr Phe Trp Phe Thr Leu Glu Cys Gly Leu

180 185 190

Leu Asp Ala Ala Asp Gly Pro Arg Ala Tyr Gly Ala Ser Val Val Ser

195 200 205

Ser Tyr Gly Glu Leu Glu His Phe Arg Ser Ala Glu Ile Arg Pro Leu

210 215 220

Asp Ile Ala Asp Met Ala His Val Asp Tyr Asp Ile Thr Gln Tyr Gln

225 230 235 240

Thr Thr Phe Tyr Ser Ala Arg Ser Leu Thr His Leu Glu Asp Val Ala

245 250 255

Gly Glu Phe Trp Ala Ser Cys Asp Asp Thr Ser Ile Glu Lys Leu Met

260 265 270

Ala Val Asp Ile

275

<210>5

<211>446

<212>PRT

<213>Streptomyces flaveolus

<400>1

Met Thr Arg Leu Ala Glu Gln Ser Ser Thr Ala Gln Gln Ser Pro Glu

1 5 10 15

Ser Glu Val Leu Asp Val Thr Gly Ile Gly Phe Gly Ala Ala Asn Leu

20 25 30

Ala Leu Ala Val Ala Leu His Glu Ser Glu Ala Ala Gly Lys Ala Leu

35 40 45

Phe Leu Glu Lys Gln Lys Glu Phe Gly Trp His Arg Gly Met Leu Leu

50 55 60

Gly Gly Ser Ser Leu Gln Val Ser Phe Leu Lys Asp Ile Ala Thr Met

65 70 75 80

Arg Asn Pro Thr Ser Asp Phe Gly Phe Leu Ser Tyr Leu Gln Glu Lys

85 90 95

Asp Arg Leu Val Asp Phe Ile Asn Gln His Thr Leu Leu Pro Ser Arg

100 105 110

Ile Glu Tyr His Asp Tyr Leu Gln Trp Ala Ala Asp Arg Leu Asn His

115 120 125

Leu Val Glu Tyr Gly Val Glu Ala Thr Gly Val Arg Pro Val Thr Glu

130 135 140

Ala Gly Glu Val Val Ala Leu Asp Val Leu Ala Gly Asp Arg Val Val

145 150 155 160

Ala Arg Thr Arg Asn Leu Val Leu Ala Ser Gly Leu Arg Pro Arg Leu

165 170 175

Pro Glu Gly Ala Glu Thr Gly Glu Arg Val Trp His Ser Ser Gln Leu

180 185 190

Leu His Arg Leu Pro Ala Phe Asp Glu Arg Pro Pro Arg Arg Ala Val

195 200 205

Val Val Gly Ala Gly Gln Ser Ala Ala Glu Val Ala Ala His Leu Met

210 215 220

Asp Arg Tyr Pro Gln Ala Glu Val Cys Ala Val Phe Ala Arg Tyr Gly

225 230 235 240

Tyr Ser Val Ala Asp Ser Ser Pro Phe Ala Asn Arg Val Phe Asp Pro

245 250 255

Ala Ala Val Asp Asp Phe Tyr Phe Ala Pro Pro Glu Val Lys Gln Ala

260 265 270

Ile Met Arg Tyr His Gly Gly Thr Asn Tyr Ala Val Val Asp Glu Asp

275 280 285

Val Leu Gln Gly Leu Tyr Arg Arg Gln Tyr Glu Gln Lys Val Ser Gly

290 295 300

Ala Pro Arg Leu Arg Val Met Asn Ala Ser Arg Leu Val Ser Val Glu

305 310 315 320

Pro Arg Gln Glu Ser Ala Ala Val Arg Val Glu Phe Leu Pro Thr Gly

325 330 335

Glu His Thr Asp Leu Asp Ala Asp Leu Val Val Tyr Ala Thr Gly Tyr

340 345 350

Asp Ser Thr Asp Pro Ala Glu Leu Leu Gly Gly Val Ser Gly Ala Leu

355 360 365

Arg Arg Asp Glu Ala Gly Glu Leu Leu Ile Gly Arg Asp Tyr Arg Leu

370 375 380

Gly Thr Thr Gly Asp Phe Arg Cys Gly Ile Tyr Val Gln Gly Ala Thr

385 390 395 400

Glu Ala Thr His Gly Ile Ala Ser Thr Leu Leu Ser Met Val Ala Val

405 410 415

Arg Ala Gly Glu Ile Ala Arg Ser Ile Thr Gly Gly Arg Cys Asp Pro

420 425 430

Asp Arg Ser Thr Gly Ser Lys Ala Ala Ala Gly Asn Arg Gly

435 440 445

<210>6

<211>231

<212>PRT

<213>Streptomyces flaveolus

<400>1

Val Tyr Glu Arg Pro Leu Tyr Arg Glu Asp Cys Asp Gly Val Val Leu

1 5 10 15

Ala Phe Leu Arg His Asn Pro Leu Ala Met Val Val Thr Ser His Asp

20 25 30

Asp Val Pro Val Ala Thr His Ala Pro Val Leu Phe Arg His Gly Pro

35 40 45

Asp Gly Ala Asp Ala Glu Ala Val Ala Ala Gly Thr Val Pro Leu Ala

50 55 60

Gly Ser Thr Leu Ile Gly His Met Asn Val Glu Asn Pro Gln Trp Arg

65 70 75 80

Arg Met Arg Ser Gly Asp Arg Ala Leu Ile Val Phe Gln Gly Pro His

85 90 95

Gly Tyr Val Ser Pro Thr Val Tyr Gly Val Thr Pro Ala Ala Pro Thr

100 105 110

Trp Asp Phe Ile Ala Val His Val Asn Gly Thr Val Glu Pro Thr Ala

115 120 125

Asp Pro Ala Ala Val Leu Asp Ile Val Ser Asp Thr Ala Arg Arg Leu

130 135 140

Glu Ser Gly Phe Gly Arg Gly Trp Asp Gln Glu Ser Ser Leu Asp Tyr

145 150 155 160

Phe Arg Gln Ile Ala Pro Gly Val Gly Ala Phe Thr Leu Arg Val Asp

165 170 175

Ser Val Gln Thr Met Phe Lys Leu Ser Gln Glu Lys Pro Ala Pro Met

180 185 190

Arg Arg Arg Val Val Glu Gln Phe Glu Ala Ser Glu Ser Gly Thr His

195 200 205

Arg Ala Leu Ala Ser Val Met Arg Asp Arg Gly Leu Thr Glu Ala Asp

210 215 220

Glu Glu Arg Glu Thr Ala Gly

225 230

<210>7

<211>3609

<212>PRT

<213>Streptomyces flaveolus

<400>1

Met Thr Gln Gly Ser Ser Arg Arg Pro Gly Leu Asp Ser Ala Leu Asn

1 5 10 15

Arg Leu Arg Glu Arg Thr Ala Arg Thr Gly Thr Thr Pro Ala Gly Ile

20 25 30

Ser Arg Val Pro Arg Asp Arg Pro Leu Glu Leu Ser Phe Ala Gln Gln

35 40 45

Arg Leu Trp Phe Leu Asp Arg Leu Met Pro Asp Ser Ala Phe Tyr Asn

50 55 60

Phe Gly Thr Ala Val Arg Val Arg Gly Pro Leu Gly Ala Gly Leu Leu

65 70 75 80

Ala Arg Ala Leu Ser Cys Val Val Ala Arg His Glu Thr Leu Arg Thr

85 90 95

Val Phe Ala Asp His Glu Gly Val Ala Gly Ala Val Val Leu Pro Pro

100 105 110

Glu Pro Val Pro Val Pro Val Thr Asp Ala Val Asp Glu Ala Asp Ala

115 120 125

Glu Arg Leu Ala Gly Glu Glu Ala Ala Arg Pro Phe Asp Leu Thr Lys

130 135 140

Gly Pro Leu Leu Arg Ala Arg Leu Leu Arg Leu Ala Asp Asp Asp His

145 150 155 160

Val Leu Leu Leu Thr Val His His Ile Ala Thr Asp Gly Trp Ser His

165 170 175

Gly Leu Leu Trp Ala Glu Leu Thr Ala Ala Tyr Thr Ala Leu Ala Asp

180 185 190

Gly Arg Gln Pro Ala Leu Pro Glu Leu Pro Val Gln Tyr Ala Asp Phe

195 200 205

Ala Ala Trp Gln Arg Arg Thr Leu Ser Pro Ala Val Leu Glu Arg Arg

210 215 220

Leu Ala Tyr Trp Gln Asp Arg Leu Ala Gly Leu Pro Pro Leu Asp Leu

225 230 235 240

Pro Leu Asp Arg Pro Arg Pro Ala Val Ala Ser Ala Glu Gly Gly Val

245 250 255

Val Thr Trp Arg Leu Pro Ala Asp Ala Val Ala Ala Ala Arg Ala Val

260 265 270

Ala Ala Arg Gln Gly Ala Thr Leu His Met Thr Leu Leu Ala Ala Phe

275 280 285

Ser Ala Val Leu Gly Arg His Ala Arg Thr Glu Asp Val Ala Val Ala

290 295 300

Gln Pro Val Ala Gly Arg Pro Leu Ala Glu Val Glu Gln Leu Ile Gly

305 310 315 320

Phe Phe Val Asn Thr Val Val Thr Arg Thr Asp Leu Gly Gly Asp Pro

325 330 335

Thr Phe Ala Glu Leu Val Glu Arg Val Arg Ala Ala Ser Val Asp Glu

340 345 350

Met Ala His Gln Asp Val Pro Phe Glu Tyr Leu Val Glu Arg Leu Val

355 360 365

Pro Glu Arg Asp Leu Ser Arg Asn Pro Leu Ala Gln Val Val Phe Gln

370 375 380

Tyr Val Pro Arg Pro Ala Ala Arg Pro Ala Pro Phe Pro Gly Thr Thr

385 390 395 400

Ala Glu Pro Phe Ala Gly Asp Arg Ala Phe Thr Arg Met Asp Leu Glu

405 410 415

Val Tyr Leu Gly Glu Asp Ala Glu Gly Gly Val Glu Gly Leu Ile Asn

420 425 430

Tyr Ser Arg Ala Leu Phe Asp Arg Glu Thr Val Glu Arg Leu Ala Arg

435 440 445

His Leu Thr Ala Leu Leu Arg Ala Ala Cys Ala Glu Pro Asp Arg Pro

450 455 460

Leu Ser Arg Leu Thr Met Thr Asp Ala Gly Glu Asp Ala Ala Leu Asp

465 470 475 480

Arg Ala Ala Arg Gly Thr Gly Val Pro Leu Pro Glu Ala Ser Leu Pro

485 490 495

Glu Leu Phe Ala Ala Gln Ala Ala Arg Thr Pro Asp Ala Val Ala Val

500 505 510

Ala Asp Gly Thr Glu His Leu Thr Tyr Ala Gln Leu Asp Arg Ala Ala

515 520 525

Asn Arg Leu Ala His Val Leu Ala Gly His Gly Ala Gly Pro Glu Ser

530 535 540

Val Val Ala Leu Ala Thr Glu Arg Ser Ala His Leu Val Val Ala Val

545 550 555 560

Leu Ala Val Leu Lys Ala Gly Gly Ala Tyr Leu Pro Leu Asp Ala Arg

565 570 575

Asn Pro Ala Ala Arg Thr Arg Ala Val Leu Ala Asp Thr Gly Ala Ala

580 585 590

Leu Leu Leu Thr Asp Gly Gly Pro Ala Pro Ala Gly Thr Glu His Leu

595 600 605

Pro Ala Val Asp Leu Arg Ala Val Pro Gly Thr Ala Pro Asp Thr Ala

610 615 620

Leu Pro Asn Thr Val Gly Pro Asp Gly Leu Ala Tyr Val Met Ser Thr

625 630 635 640

Ser Gly Ser Thr Gly Thr Pro Lys Ala Val Ala Thr Thr His Arg Ala

645 650 655

Val Ala Ala Leu Ala Leu His Arg Arg Trp Ser Gly Gly Ala His Glu

660 665 670

Arg Val Leu Leu His Ser Pro Gln Ala Phe Asp Ala Ser Thr Tyr Glu

675 680 685

Leu Trp Ser Pro Leu Leu Ser Gly Arg Arg Val Val Val Ala Pro Pro

690 695 700

Gly Ala Leu Gly Pro Ala Ala Leu Ala Arg Val Val Ala Asp Gln Gly

705 710 715 720

Val Thr Ala Leu Trp Leu Thr Ser Gly Leu Phe Asp Leu Val Val Glu

725 730 735

Glu Asp Val Thr Cys Leu Ala Gly Val Arg Glu Leu Val Val Gly Gly

740 745 750

Asp Thr Val Ser Pro Ala Thr Val Ala Arg Val Arg Gly Ala His Pro

755 760 765

Asp Leu Thr Val Val Asn Gly Tyr Gly Pro Thr Glu Thr Thr Thr Phe

770 775 780

Ala Thr Leu His Pro Ile Ala Pro Ala Asp Pro Ala Pro Gly Gly Arg

785 790 795 800

Val Pro Ile Gly Ser Pro Leu Asp Asn Thr Arg Ala His Val Leu Asp

805 810 815

Asp Arg Leu Arg Pro Val Pro Phe Gly Val Pro Gly Glu Leu Tyr Val

820 825 830

Gly Gly Pro Arg Leu Ala Arg Gly Tyr Ala Gly Arg Pro Ala Ala Thr

835 840 845

Ala Glu Arg Phe Leu Pro Asp Pro Ser Gly Pro Ala Gly Ser Arg Met

850 855 860

Tyr Arg Thr Gly Asp Val Val Arg Arg Arg Pro Asp Gly Val Leu Glu

865 870 875 880

Phe Leu Gly Arg Ala Asp Asp Gln Ala Lys Leu Arg Gly Leu Arg Val

885 890 895

Glu Pro Gly Glu Val Glu Ala Val Leu Ala Ala His Pro Ala Val Ala

900 905 910

His Ala Ala Val Val Val Arg Gly Asp Gly Pro Ala Gly Lys Arg Leu

915 920 925

Val Ala His Val Val Pro Arg Ala Gly Arg Thr Thr Asp Thr Ala Ala

930 935 940

Leu Arg Ala His Ala Ala Ala Ala Leu Pro Asp Tyr Leu Val Pro Ser

945 950 955 960

Ala Phe Val Leu Ala Asp Ala Leu Pro Leu Thr Ala Thr Gly Lys Val

965 970 975

Asp Arg Ala Ala Leu Pro Ala Pro Ala Glu Thr Ala Asp Ala Gly Leu

980 985 990

Ala Ala Pro Arg Thr Asp Ala Glu Arg Ala Leu Cys Glu Ile Phe Ala

995 1000 1005

Glu Leu Leu Asp Ala Asp Ala Phe Gly Ala Glu Asp Asp Phe Phe Val

1010 1015 1020

Arg Gly Gly His Ser Leu Leu Ala Thr Arg Leu Val Ala Arg Ile Ala

1025 1030 1035 1040

Arg Ala Phe Gly Thr Glu Val Pro Leu Arg Glu Val Phe Glu His Arg

1045 1050 1055

Thr Pro Arg Ala Leu Ala Ser Val Val Ala Gly Ala Ala Leu Pro Ala

1060 1065 1070

Asp Pro Ala Pro Pro Leu Val Pro Ala Asp Arg Asp Arg Leu Leu Pro

1075 1080 1085

Leu Ser Phe Ala Gln Gln Arg Met Trp Phe Leu Asp Gln Leu Ala Pro

1090 1095 1100

Gly Ser Ala Ser Tyr Thr Ser Gly Gly Ala Leu Arg Val Arg Gly Pro

1105 1110 1115 1120

Leu Asp Pro Glu Arg Leu Ala Gly Ala Leu Ser Ala Val Val Ala Arg

1125 1130 1135

His Glu Thr Leu Arg Thr Thr Phe Thr Val Ala Asp Gly Val Pro Ala

1140 1145 1150

Ala Val Ile Gly Ala Ala Ala Pro Val Ala Pro Arg Ile Val Asp Val

1155 1160 1165

Pro Asp Ala Asp Ala Ala Arg Ala Ala Ala Ser Ala Glu Leu Ser Thr

1170 1175 1180

Gly Phe Asp Leu Thr Arg Gly Pro Leu Leu Arg Ala Thr Leu Leu Arg

1185 1190 1195 1200

Leu Ala Pro Asp Asp His Val Leu Val Val Ala Val His His Ile Ala

1205 1210 1215

Thr Asp Gly Trp Ser Gln Ala Leu Leu Trp Ala Glu Ile Ala Ala Ala

1220 1225 1230

Tyr Asp Gly Ala Pro Leu Pro Glu Leu Pro Val Gln Tyr Gly Asp His

1235 1240 1245

Ala Val Trp Gln Arg Ser Trp Leu Thr Gly Glu Val Leu Glu Arg Arg

1250 1255 1260

Ala Gly Tyr Trp Thr Gly Arg Leu Ala Gly Leu Ala Pro Leu Glu Leu

1265 1270 1275 1280

Pro Leu Asp Lys Ala Arg Pro Ala Val Ala Thr Gly Arg Ala Gly Thr

1285 1290 1295

Leu Pro Trp Gln Leu Pro Ala Glu Leu Ile Arg Asp Ala Arg Ala Val

1300 1305 1310

Ala Ala Arg Glu Gly Ala Thr Leu Tyr Met Val Leu Leu Ala Ala Phe

1315 1320 1325

Thr Leu Val Leu Ser Arg Tyr Ala Arg Thr Glu Asp Ile Ala Val Gly

1330 1335 1340

Ser Pro Thr Ala Gly Arg Thr Arg Ala Glu Thr Glu Ala Leu Ile Gly

1345 1350 1355 1360

Phe Phe Val Asn Val Val Ala Val Arg Thr Asp Leu Ser Gly Asp Pro

1365 1370 1375

Thr Phe Arg Glu Leu Leu Gly Arg Val Arg Glu Ser Val Val Gly Ala

1380 1385 1390

Val Glu His Gln Asp Val Pro Phe Glu His Leu Val Glu Arg Leu Arg

1395 1400 1405

Pro Glu Arg Asp Leu Ser Arg Asn Pro Leu Val Gln Val Ala Phe Gln

1410 1415 1420

Leu Leu Ala Asp Ala Pro Arg Arg Pro Trp Trp Gln Gly Ala Arg Ala

1425 1430 1435 1440

Glu Pro Phe Asp Ile Asp His Ala Tyr Thr Arg Met Asp Leu Glu Val

1445 1450 1455

His Ala Val Glu Thr Gly Asp Glu Val Gly Ala Thr Val Leu Tyr Ala

1460 1465 1470

Ala Asp Leu Phe Asp Ala Asp Thr Val Arg Gln Leu Met His His Val

1475 1480 1485

Ser Val Val Leu Gly Glu Val Leu Ala Asp Pro Asp Arg Pro Val Ser

1490 1495 1500

Ala Ala Thr Met Leu Asp Glu Thr Asp Arg His Arg Thr Leu Val Ala

1505 1510 1515 1520

Trp Asn Asp Thr Ala Ala Pro Leu Pro Asp Gly Cys Val Pro Arg Leu

1525 1530 1535

Tyr Ala Glu Gln Ala Ala Arg Thr Pro Asp Ala Val Ala Leu Ile Cys

1540 1545 1550

Gly Asp Glu Arg Val Thr Tyr Ala Glu Leu Asp Arg Arg Ala Asn Arg

1555 1560 1565

Phe Ala His Leu Leu Leu Ala His Gly Val Gly Ala Asp Glu Pro Val

1570 1575 1580

Gly Val Ala Thr Gly Arg Ser Thr Gly Met Val Ala Ala Val Leu Gly

1585 1590 1595 1600

Val Leu Lys Ala Gly Ala Ala Tyr Val Pro Leu Asp Pro Arg Asn Pro

1605 1610 1615

Pro Gly Arg Thr Glu Arg Ile Val Ala Thr Ser Gly Leu Arg Val Val

1620 1625 1630

Ile Ala Asp Arg Pro Val Pro Gly Thr Asp Gly Ile Thr Val Leu Asp

1635 1640 1645

Val Thr Asp Pro Gly Pro Gly Pro Asp Thr Asp Pro Gly Ile Asp Pro

1650 1655 1660

His Pro Asp Thr Thr Ala Tyr Val Ile Tyr Thr Ser Gly Ser Ser Gly

1665 1670 1675 1680

Glu Pro Lys Gly Val Ala Val Thr His Arg Asn Val Val Val Leu Ala

1685 1690 1695

Ala Asp Arg Arg Trp Ser Asn Gly Asn His Glu Arg Val Leu Leu His

1700 1705 1710

Tyr Pro Leu Ala Thr Asp Ile Ser Thr Tyr Glu Leu Trp Pro Phe Leu

1715 1720 1725

Leu Thr Gly Lys Gln Ile Val Val Ala Thr Asp Glu His Val Glu Pro

1730 1735 1740

His Thr Phe Asp Arg Leu Ile Arg Glu His Gly Val Thr Ala Met Cys

1745 1750 1755 1760

Leu Pro Ala Pro Leu Phe Ser Leu Leu Ala Glu Glu Cys Met Glu Cys

1765 1770 1775

Phe Gly Gly Leu Arg Glu Val Leu Thr Gly Gly Glu Ala Val Ser Gly

1780 1785 1790

Glu Thr Val Ala Gln Val Met Ala Ala His Pro His Leu Thr Val Ala

1795 1800 1805

Asp Ala Tyr Gly Pro Thr Glu Ala Thr Ala Phe Thr Thr Leu Phe Pro

1810 1815 1820

Met Glu Pro Gly Phe Arg Leu Ala Gly Ser Arg Val Pro Ile Gly Ala

1825 1830 1835 1840

Pro Ile Asp Asn Thr Arg Val Tyr Val Leu Asp Asp Thr Leu Arg Pro

1845 1850 1855

Ala Pro Leu Gly Val Ala Gly Glu Leu Val Ile Gly Gly Pro Arg Val

1860 1865 1870

Ala Arg Gly Tyr Leu Gly Arg Pro Asp Leu Thr Ala Glu Lys Phe Val

1875 1880 1885

Pro Asp Pro Trp Gly Gly Pro Gly Glu Arg Met Tyr Arg Thr Gly Asp

1890 1895 1900

Val Val Arg Trp Leu Pro Gly Gly Ala Leu Glu Phe Leu Gly Arg Ala

1905 1910 1915 1920

Asp Val Gln Val Lys Ile Arg Gly Phe Arg Val Glu Pro Gly Glu Val

1925 1930 1935

Glu Ala Ala Leu Leu Arg His Pro Ala Val Ser Gln Val Thr Val Ala

1940 1945 1950

Val Arg Glu Asp Ile Pro Gly Asp Lys Arg Leu Val Ala Tyr Val Val

1955 1960 1965

Leu Glu Pro Glu Ala Gly Ala Ser Val Leu Pro Ala Leu Arg Ala His

1970 1975 1980

Ala Ala Gly Ser Val Pro Asp Tyr Met Val Pro Ser Ala Phe Val Ala

1985 1990 1995 2000

Leu Asp Ala Phe Pro Leu Ser Thr Thr Gly Lys Ile Asp Arg Arg Ala

2005 2010 2015

Leu Pro Ala Pro Asp Thr Arg Ser Val Ala Glu Ser Gly Tyr Val Pro

2020 2025 2030

Pro Ala Thr Glu Ala Glu Arg Val Leu Cys Glu Ile Phe Ala Glu Val

2035 2040 2045

Leu Asp Val His Pro Val Gly Ala Asp Asp Asp Phe Phe Ala Leu Gly

2050 2055 2060

Gly His Ser Leu Leu Ala Thr Arg Ala Ile Ala Arg Ile Arg Ala Arg

2065 2070 2075 2080

Phe Gly Pro Asp Val Pro Leu Gln Ala Val Phe Glu Arg Arg Ser Pro

2085 2090 2095

Arg Arg Leu Ala Glu Thr Leu Gly Glu Pro Gly Thr Ala Thr Asp Val

2100 2105 2110

Ile Arg Pro Ala Arg Arg Asp Gly Ala Ala Leu Pro Leu Ser Ser Ser

2115 2120 2125

Gln Arg Arg Leu Trp Phe Leu Asp Arg Leu Thr Pro Asp Ser Gly Phe

2130 2135 2140

Trp Asn Val Ala Met Gly Val Arg Ala His Gly Pro Leu Asp Val Asp

2145 2150 2155 2160

Ala Leu Gly Arg Ala Leu Ser Leu Val Val Ser Arg His Glu Ala Leu

2165 2170 2175

Arg Thr Val Phe Ala Ala Asp Ala Gly Glu Pro Met Ala Val Val Arg

2180 2185 2190

Pro Ala Thr Pro Leu Arg Leu Glu Val Thr Asp Val Ala Asp Glu Ala

2195 2200 2205

Glu Val Arg Ala Leu Ala Glu Ala Asp Ala Ala Arg Pro Phe Asp Leu

2210 2215 2220

Ala Arg Gly Pro Leu Leu Arg Ala Arg Val Leu Arg Leu Ala Ala Glu

2225 2230 2235 2240

Asp His Ala Val Leu Ile Thr Ala His His Ala Val Thr Asp Gly Trp

2245 2250 2255

Ser His Ala Val Phe Trp Gly Glu Leu Ala Glu Ala Tyr Arg Ala Glu

2260 2265 2270

Leu Ser Gly Asp Pro Ala Glu Leu Pro Glu Leu Pro Val Gln Tyr Gly

2275 2280 2285

Asp Phe Ala Val Trp Gln Gln Gly Arg Leu Thr Gly Ala Glu Leu Glu

2290 2295 2300

Arg Tyr Leu Thr Tyr Trp Arg Ala Arg Leu Ala Gly Leu Arg Pro Leu

2305 2310 2315 2320

Glu Leu Pro Leu Asp Arg Pro Arg Pro Ala Val Ala Gly Ser Ala Gly

2325 2330 2335

Ala Ser Gln Pro Trp Glu Leu Pro Glu Asp Leu Val Arg Ala Ala Arg

2340 2345 2350

Ala Phe Gly Asp Thr Glu Gly Ala Thr Leu Tyr Met Thr Leu Leu Thr

2355 2360 2365

Ala Phe Thr Val Val Leu Ala Arg Phe Ala Gly Thr Glu Asp Val Ala

2370 2375 2380

Val Gly Ala Pro Val Ala Gly Arg Thr Arg Pro Glu Val Glu Arg Leu

2385 2390 2395 2400

Ile Gly Phe Phe Val Asn Met Leu Val Leu Arg Thr Asp Val Ser Gly

2405 2410 2415

Asp Pro Thr Phe Arg Asp Leu Leu Gly Arg Val Arg Glu Thr Val Val

2420 2425 2430

Gly Ala Met Asp His Gln Asp Leu Pro Phe Glu His Leu Val Glu Thr

2435 2440 2445

Leu Ala Pro Glu Arg Asp Leu Ser Arg Asn Pro Leu Val Gln Val Val

2450 2455 2460

Phe Gln Leu Met Arg Ala Pro Gly Asp Lys Gly Asp Arg Leu Gly Ala

2465 2470 2475 2480

Ala Arg Leu Glu Pro Leu Leu Asp Glu His Ala Phe Thr Arg Val Asp

2485 2490 2495

Leu Glu Val His Leu Thr Glu Asp Gly Asp Arg Val Arg Gly Thr Val

2500 2505 2510

Leu His Ser Thr Ala Leu Phe Glu Ala Asp Thr Val Arg Arg Leu Leu

2515 2520 2525

His His His Thr Val Leu Leu Arg Ala Ala Leu Ala Asp Pro Asp Ala

2530 2535 2540

Pro Leu Ser Ala Leu Ser Leu Leu Asp Asp Asp Asp Arg Arg Leu Leu

2545 2550 2555 2560

Leu Glu Arg Trp Asn Asp Thr Ala Leu Pro Tyr Arg Asp Val Pro Leu

2565 2570 2575

Val Glu Leu Phe Ala Glu Gln Val Ala Arg Thr Pro Gly Ala Arg Ala

2580 2585 2590

Val Glu Cys Glu Asp Asp Val Leu Thr Tyr Ala Ala Leu Asp His Glu

2595 2600 2605

Ala Glu Arg Ile Ala Ala Gly Leu Arg Ala Gln Gly Val Gly Pro Asp

2610 2615 2620

Asp Leu Val Gly Leu Cys Leu Glu Arg Gly Thr Val Gln Met Ala Ala

2625 2630 2635 2640

Leu Ile Gly Ile Leu Lys Ala Gly Ala Ala Tyr Val Pro Ile Asp Pro

2645 2650 2655

Ser His Pro Arg Asp Arg Ile Arg Leu Ile Val Asp Asp Ala Arg Met

2660 2665 2670

Thr Val Ala Val Thr Asp Arg Ala His Ala Asp Val Phe Ala Glu Gly

2675 2680 2685

Thr Ala Leu Val Leu Val Asp Ala Pro Ala Gly Gln Glu Pro Pro Ala

2690 2695 2700

Ala Thr Ala Ala Gly Arg Pro Gly Pro Asp Ser Leu Ala Tyr Val Val

2705 2710 2715 2720

Tyr Thr Ser Gly Ser Thr Gly Val Pro Lys Gly Ile Ala Met Pro Ala

2725 2730 2735

Arg Cys Val Val Asn Met Leu Ala Trp Gln Lys Lys Thr Val Pro Gly

2740 2745 2750

Gly Pro Gly Thr Arg Thr Ala Gln Phe Thr Ala Leu Thr Phe Asp Val

2755 2760 2765

His Val Gln Glu Val Leu Ser Ala Leu Leu Tyr Gly Glu Thr Leu Val

2770 2775 2780

Ile Pro Thr Glu Glu Thr Arg Arg Asp Pro Ala Arg Phe Ala Arg Trp

2785 2790 2795 2800

Leu Asp Glu Arg Ala Val Glu Gln Ile Phe Val Pro Asn Leu Met Ile

2805 2810 2815

Arg Ala Leu Ala Glu Glu Ala Gly Ala Gly Arg Ala Arg Leu Thr Ser

2820 2825 2830

Leu Arg His Ile Ser Gln Ala Gly Glu Pro Leu Ser Leu Asp Thr Val

2835 2840 2845

Leu Arg Glu Phe Cys Ala Ala Arg Pro Arg Leu Arg Leu His Asn His

2850 2855 2860

Tyr Gly Ser Thr Glu Ile Gln Val Val Thr Ser Phe Thr Leu Pro Ala

2865 2870 2875 2880

Asp Val Ala Asp Trp Pro Arg Thr Ala His Leu Gly Glu Pro Val Asp

2885 2890 2895

Asn Ser Arg Ala Tyr Val Leu Asp Asp Arg Leu Arg Pro Val Pro Val

2900 2905 2910

Gly Val Ala Gly Glu Leu Cys Phe Ala Gly Pro Gly Leu Ala Arg Gly

2915 2920 2925

Tyr Val Gly Lys Pro Glu Leu Thr Ala Gln Lys Phe Val Pro Asp Pro

2930 2935 2940

Phe Gly Pro Pro Gly Ser Arg Leu Tyr Arg Thr Gly Asp Leu Gly Arg

2945 2950 2955 2960

Trp Arg Pro Asp Gly Ser Leu Glu Tyr Leu Gly Arg Leu Asp His Gln

2965 2970 2975

Val Lys Ile Arg Gly Phe Arg Val Glu Leu Gly Glu Val Glu Ala Val

2980 2985 2990

Leu Leu Arg His Pro Glu Val Thr Arg Ala Val Ile Val Ala Arg Glu

2995 3000 3005

Asp Ala Pro Gly Val Lys Arg Leu Val Gly Tyr Val Val Pro Val Pro

3010 3015 3020

Gly Thr Asp Gly Gly Leu Pro Ala Arg Leu Arg Ala His Leu Ala Asp

3025 3030 3035 3040

Ala Val Pro Asp Tyr Met Val Pro Ser Ala Leu Val Ala Leu Asp Ala

3045 3050 3055

Phe Pro Leu Thr Thr Thr Gly Lys Ile Asp Arg Ala Ala Leu Pro Ala

3060 3065 3070

Pro Asp Leu Arg Thr Thr Leu Asp Thr Gly Phe Thr Ala Pro Arg Thr

3075 3080 3085

Gly Ala Glu Gln Thr Leu Cys Glu Val Phe Ala Glu Val Leu Glu Thr

3090 3095 3100

Gly Gly Val Gly Ile Asp Asp Asp Phe Phe Ala Leu Gly Gly His Ser

3105 3110 3115 3120

Leu Val Ala Ala Arg Thr Val Ala Arg Ile Arg Glu Ala Leu Gly Ala

3125 3130 3135

Glu Val Ser Leu Arg Glu Leu Phe Gln His Arg Thr Pro Arg Ala Leu

3140 3145 3150

Ala Glu Val Val Ala Val Ala Pro Arg Thr Ala Val Pro Pro Leu Val

3155 3160 3165

Pro Val Ser Arg Gln Glu Pro Leu Pro Leu Ser Leu Gly Gln Leu Arg

3170 3175 3180

Leu Trp Arg Leu His Glu Ala Asp Pro Gly Asp Pro Val Trp Thr Ile

3185 3190 3195 3200

Pro Leu Ala Val Arg Ile Thr Gly Glu Leu Asp Ala Asp Leu Leu Gly

3205 3210 3215

Arg Ala Leu Thr Glu Val Val Arg Arg His Glu Ala Leu Arg Thr Val

3220 3225 3230

Phe Val Pro Gly Asp Glu Pro Ala Ser Val Ile Leu Pro Ala Thr Asp

3235 3240 3245

Ile Val Leu Asp Pro Val Asp Val Ala Asp Glu Thr Ala Ala Arg Ala

3250 3255 3260

Leu Ala Asp Glu Ala Ala Ala Arg Pro Phe Asp Leu Val Arg Gly Pro

3265 3270 3275 3280

Val Leu Arg Pro Ala Leu Leu Arg Ile Ala Pro Asp Asp His Val Leu

3285 3290 3295

Leu Leu Thr Val His His Ile Ala Thr Asp Gly Trp Ser Gln Gly Val

3300 3305 3310

Leu Trp Thr Glu Leu Ser Gly Ala Tyr Ala Ala Leu Arg Glu Asn Arg

3315 3320 3325

Pro Ala Glu Leu Pro Glu Leu Pro Val Gln Tyr Gly Asp Phe Ala Phe

3330 3335 3340

Trp Gln Arg Ser Trp Leu Thr Gly Ala Ala Leu Asp Ala Gln Leu Gly

3345 3350 3355 3360

His Trp Arg Arg Arg Leu Asp Gly Leu Arg Pro Leu Ala Leu Pro Gly

3365 3370 3375

Val Pro Ala Asp Ala Ala His Asp Ala Thr Gly Val Leu Thr Glu Trp

3380 3385 3390

Arg Leu Pro Ala Gly Leu Val Ala Thr Ala Arg Arg Val Gly Ala Glu

3395 3400 3405

His Asp Ala Thr Leu Tyr Met Thr Leu Leu Ala Ala Phe Thr Ala Thr

3410 3415 3420

Leu Ala Arg Trp Ala Gly Thr Asp Asp Leu Ala Val Gly Ser Pro Val

3425 3430 3435 3440

Ala Gly Arg Thr Arg Ala Glu Val Glu Gly Leu Ile Gly Phe Phe Ala

3445 3450 3455

Asn Phe Val Pro Leu Arg Val Asp Leu Ser Gly Asp Pro Ser Phe Ala

3460 3465 3470

Gly Leu Leu Glu Arg Val Arg Asp Thr Ala Leu Asp Ala Tyr Ala His

3475 3480 3485

Gln Ala Leu Pro Trp Glu Arg Val Val Glu Gly Leu Gly Leu Asp Pro

3490 3495 3500

Glu Gln Pro Leu Val Asp Val Val Phe Gln Leu Val Asn Val Glu Leu

3505 3510 3515 3520

Gly Glu Leu Gly Leu Pro Gly Ala Arg Val Glu Gln Phe Thr Gly Gln

3525 3530 3535

Gln Ala Tyr Ala Arg Trp His Leu Glu Val His Leu Val Glu Asp Pro

3540 3545 3550

Asp Gly Gly Leu Thr Gly His Val Val His Arg Ala Ala Ala Leu Gly

3555 3560 3565

Arg Arg Val Val Asp Gly Leu Leu Ala Gly Thr Ala Ala Leu Leu Gln

3570 3575 3580

Ala Ala Leu Ala Glu Pro Gly Leu Pro Val Ser Ala Leu His Ala Pro

3585 3590 3595 3600

Asp Pro Leu Glu Gly Thr Thr His Asp

3605

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Met Thr Glu Pro Leu Thr Asp Pro Lys Ala Ala Gln Pro Pro Ala Leu

1 5 10 15

Ala His Arg Leu Ala Gly Leu Pro Glu Pro Glu Arg Arg Arg Val Val

20 25 30

Leu Asp Leu Val Arg Thr Gln Val Ala Glu Val Leu Asp Leu Gly Thr

35 40 45

Ala Ala Asp Val Pro Pro Asp Arg Ala Ile Arg Glu Leu Gly Leu Arg

50 55 60

Ser Leu Thr Ala Val Gln Leu Leu Leu Arg Leu Ser Arg Gly Thr Gly

65 70 75 80

Val Lys Leu Pro Thr Thr Ala Ile Tyr Asp His Pro Thr Ala Arg Ala

85 90 95

Leu Ala Asp Val Leu Val Asp Ala Ala Ser Gly Arg His Val Arg Leu

100 105 110

Ala Glu Asp Asp Glu Pro Val Gln Arg Ala Ala Asp Asp Asp Pro Val

115 120 125

Ala Val Val Gly Met Ala Cys Arg Phe Pro Gly Gly Val Thr Thr Pro

130 135 140

Asp Glu Leu Trp Arg Leu Val Leu Glu Glu Arg Asp Ala Ile Thr Pro

145 150 155 160

Phe Pro Ala Asp Arg Gly Trp Asp Leu Ala Ala Leu Ala Asp Pro Asp

165 170 175

Gly Pro Ser Ala Ser Arg Thr Arg His Gly Gly Phe Leu Asp Asp Val

180 185 190

Ala Leu Phe Asp Ala Gly Phe Phe Gly Ile Ser Pro Arg Glu Ala Gln

195 200 205

Leu Met Asp Pro Gln Gln Arg Leu Leu Leu Glu Thr Ser Trp Glu Ala

210 215 220

Leu Glu Arg Ala Gly Val Ala Pro Gly Ser Trp Arg Gly Gly Arg Val

225 230 235 240

Gly Val Phe Val Gly Ala Asn Ala Gln Ser Tyr Ser Ser Leu Leu Ala

245 250 255

Gly Ile Pro Glu Gly Gly Asp Gly His Ala Leu Thr Gly Arg Leu Ala

260 265 270

Ser Val Val Ala Gly Arg Ile Ser Tyr Val Leu Gly Leu Glu Gly Pro

275 280 285

Ala Phe Thr Val Asp Thr Ala Cys Ser Ser Ser Leu Val Ala Leu His

290 295 300

Gln Ala Val Arg Ser Leu Arg Ser Gly Glu Ser Thr Leu Ala Leu Ala

305 310 315 320

Gly Gly Val Thr Val Met Pro Thr Pro Glu Leu Phe Val Asp Phe Thr

325 330 335

Lys Gln Gly Gly Leu Ser Glu Asp Gly Arg Cys Arg Ser Tyr Ala Lys

340 345 350

Ala Ala Asp Gly Leu Gly Trp Ser Glu Gly Val Gly Met Leu Leu Leu

355 360 365

Glu Arg Leu Ser Asp Ala Arg Arg Asn Gly His Pro Val Leu Ala Leu

370 375 380

Leu Pro Gly Thr Ala Val Asn Gln Asp Gly Ala Ser Asn Gly Leu Thr

385 390 395 400

Ala Pro Ser Gly Ala Ala Gln Gln Arg Val Val Arg Gln Ala Leu Ala

405 410 415

Asp Ala Gly Leu Arg Pro Ala Asp Val Asp Ala Val Glu Gly His Gly

420 425 430

Thr Gly Thr Ala Leu Gly Asp Pro Ile Glu Ala Gln Ala Leu Leu Ser

435 440 445

Ser Tyr Gly Gln Asp Arg Asp Arg Pro Leu Trp Leu Gly Ser Leu Lys

450 455 460

Ser Asn Leu Gly His Ala Gln Ala Ala Ala Gly Val Gly Gly Val Ile

465 470 475 480

Lys Thr Val Leu Ala Leu Arg His Gly Leu Leu Pro Lys Thr Leu His

485 490 495

Val Asp Glu Pro Thr Pro His Val Asp Trp Val Ser Gly Asp Val Arg

500 505 510

Leu Leu Thr Glu Ala Arg Pro Trp Pro Arg Gly Glu Arg Pro Arg Arg

515 520 525

Ala Gly Val Ser Ser Phe Gly Val Ser Gly Thr Asn Ala His Val Ile

530 535 540

Val Ala Glu Ala Pro Glu Pro Glu Glu Ala Thr Ala Pro Pro Ala Gly

545 550 555 560

Ala Leu Pro Val Pro Trp Gln Leu Ser Ala Arg Thr Glu Ala Ala Leu

565 570 575

Leu Glu Gln Ala Ala Arg Leu Arg Glu Phe Val Ala Ala Asp Pro Gly

580 585 590

Leu Glu Pro Ala Ser Val Gly Phe Thr Leu Ala Thr Arg Arg Thr Ala

595 600 605

Phe Glu His Arg Ala Ala Val Val Gly Arg Asp Arg Ala Glu Leu Leu

610 615 620

Ala Ala Leu Asp Val Leu Ala Ala Gly Gly Ala Asp Pro Ala Val Val

625 630 635 640

Arg Gly Thr Ala Gly Ala Asp Gly Ser Val Val Phe Val Phe Ala Gly

645 650 655

Gln Gly Gly Gln Trp Leu Gly Met Gly Val Glu Leu Leu Asp Thr His

660 665 670

Pro Val Phe Ala Ala Arg Met Ala Glu Cys Glu Arg Ala Leu Ala Pro

675 680 685

Tyr Leu Asp Trp Ser Val Val Asp Val Leu Arg Gly Ala Glu Asp Ala

690 695 700

Pro Pro Leu Ser Arg Val Asp Val Val Gln Pro Val Leu Phe Ala Leu

705 710 715 720

Met Val Ser Leu Ala Ala Val Trp Arg Ser His Gly Val Val Pro Ala

725 730 735

Ala Val Val Gly His Ser Gln Gly Glu Ile Ala Ala Ala Cys Val Ala

740 745 750

Gly Ala Leu Thr Leu Asp Asp Ala Ala Lys Thr Val Ala Leu Arg Ala

755 760 765

Lys Ala Val Ala Asp Leu Pro Gly Arg Cys Gly Met Ala Phe Val Ala

770 775 780

Ala Pro Ala Ala Asn Val Glu Arg Met Leu Glu Arg Trp Pro Gly Arg

785 790 795 800

Leu Gly Ile Ala Ala Ala Asn Ser Pro Arg Ser Leu Val Val Ala Gly

805 810 815

Asp Arg Asp Ala Leu Glu Glu Leu Leu Asp Leu Cys Asp Asp Glu Gly

820 825 830

Leu Arg Ala Gly Arg Val Ala Ala Asp Tyr Ala Ser His Ser Pro Gln

835 840 845

Val Glu Ala Val Arg Gln Arg Leu Leu Ala Asp Leu Lys Gly Ile Arg

850 855 860

Pro Arg Asp Gly Asp Val Pro Leu Tyr Ser Thr Val Thr Ala Asp Trp

865 870 875 880

Ile Asp Gly Ser Glu Leu Gly Ala Arg Tyr Trp Tyr Arg Asn Leu Arg

885 890 895

Glu Pro Val Leu Phe Gln Asn Ala Ile Ser Asp Leu Leu Ala Ala Gly

900 905 910

His His Gly Phe Val Glu Ile Ser Pro His Pro Val Leu Thr Val Ala

915 920 925

Met Gln Gln Thr Ala Glu Ala Ala Gly Thr Glu Leu Arg Val Ala Ala

930 935 940

Thr Leu Arg Arg Asp Glu Ser Asp Arg Leu Arg Leu Thr Thr Ala Leu

945 950 955 960

Ala Glu Val Ala Ala Asp Gly Val Pro Val Asp Trp Arg Pro Leu Phe

965 970 975

Asp Ala Thr Gly Ala Arg Pro Val Glu Leu Pro Ala Tyr Pro Phe Gln

980 985 990

Arg Glu Arg Tyr Trp Leu Thr Pro Gln Ala Ala Ala Gly Asp Leu Thr

995 1000 1005

Ala Val Gly Leu Thr Ala Gly Gly His Pro Leu Leu Ala Ala Glu Ala

1010 1015 1020

Glu Leu Pro Asp Glu Asp Gly Leu Leu Leu Thr Gly Arg Ile Ser Pro

1025 1030 1035 1040

Glu Thr His Pro Trp Leu Thr Glu His Thr Val Leu Gly Thr Ala Leu

1045 1050 1055

Leu Pro Gly Thr Ala Val Leu Glu Met Val Ala His Ala Gly His Arg

1060 1065 1070

Leu Asp Arg Ala Gln Ile Glu Glu Leu Thr Leu Ala Ala Pro Ile Gly

1075 1080 1085

Val Pro Ala Asp Gly Leu Thr Leu Arg Val Thr Val His Gly Ala Asp

1090 1095 1100

Gly Ser Gly Arg Arg Thr Val Ala Val His Ser His Ser Gly Asp Gly

1105 1110 1115 1120

Trp Thr Arg His Ala Thr Gly Val Leu Ala Pro Ala Glu Pro Ala Glu

1125 1130 1135

Pro Ala Glu Pro Glu Thr Gly Ala Trp Pro Pro Ala Gly Ala Glu Pro

1140 1145 1150

Val Pro Val Asp Gly Val Tyr Asp Arg Phe Ala Ala Arg Gly Tyr Gly

1155 1160 1165

Tyr Gly Pro Ala Phe Gln Gly Leu Arg Ala Leu Trp Arg Arg Asp Thr

1170 1175 1180

Glu Val Tyr Ala Glu Val Glu Leu Pro Asp Thr Val Asp Thr Ala Gly

1185 1190 1195 1200

His Leu Val His Pro Ala Leu Leu Asp Ala Val Thr Gln Ala Val Pro

1205 1210 1215

Ala Ala Glu Pro Glu Asp Ala Pro Leu Leu Leu Pro Phe Thr Trp Thr

1220 1225 1230

Gly Val Thr Val His Pro Gly Pro Ala Arg Val Leu Arg Val Arg Ala

1235 1240 1245

Ala Arg Glu Ser Ala Asp Thr Leu Ser Leu Thr Ala Thr Asp Arg Glu

1250 1255 1260

Gly Arg Pro Val Val Glu Leu Ala Ala Leu Arg Leu Arg Pro Ala Ser

1265 1270 1275 1280

Ala Gly Gln Leu Arg Ala Val Ala Ala Ala Gly Thr Arg Asp Ala Leu

1285 1290 1295

Phe Arg Val Thr Trp Gln Thr Pro Asp Ala Glu Thr Pro Ala Ala Ala

1300 1305 1310

Arg Cys Ala Val Leu Gly Asp Gly Pro Glu Gly Leu Ala Ala Pro Leu

1315 1320 1325

Ser Thr Ala Leu Ala Asp Ile Asp Ala Gly Ala Val Asp Leu Val Leu

1330 1335 1340

Ala Pro Val Ser Thr Gly Ala Asp Val Val Ala Ala Ala His Arg Ala

1345 1350 1355 1360

Thr Ala Gln Val Leu Glu Leu Leu His Glu Trp Leu Ala Asp Asp Arg

1365 1370 1375

Phe Gly Gln Ala Arg Leu Ala Ile Val Thr Arg His Ala Val Ala Ala

1380 1385 1390

Arg Pro Gly Glu Glu Pro Asp Pro Ala Ala Ala Ala Val Trp Gly Leu

1395 1400 1405

Val Arg Ser Ala Gln Thr Glu His Pro Asp Arg Phe Leu Leu Val Asp

1410 1415 1420

Thr Asp Gly Thr Pro Ala Ser Leu Asp Ala Val Pro Ala Val Gly Asp

1425 1430 1435 1440

Glu Pro Gln Thr Ala Leu Arg Asp Gly Glu Arg Leu Val Ala Arg Leu

1445 1450 1455

Thr Arg Ala Ala Glu Thr Ala Leu Arg Pro Pro Val Gly Ala Asp Ala

1460 1465 1470

Trp Arg Val Asp Val Val Arg Pro Gly Ser Ile Asp Gly Val Asp Ala

1475 1480 1485

Val Ala Ala Pro Asp Ala Thr Ala Pro Leu Ala Pro Gly Gln Val Arg

1490 1495 1500

Ile Ala Val Arg Ala Ala Gly Leu Asn Phe Arg Asp Val Leu Cys Ala

1505 1510 1515 1520

Leu Asp Met Tyr Pro Asp Glu Val Asp Ala Ile Gly Ser Glu Ala Ala

1525 1530 1535

Gly Thr Val Val Ala Val Ala Pro Asp Val Thr Asp Leu Ala Val Gly

1540 1545 1550

Asp Arg Val Leu Gly Met Val Pro Gly Gly Phe Gly Thr Leu Ala Val

1555 1560 1565

Val Asp Arg Arg Leu Val Val Pro Val Pro Ala Gly Trp Ser Trp Val

1570 1575 1580

Arg Ala Ala Ala Leu Pro Ser Val Phe Ala Thr Ala Trp Phe Ala Leu

1585 1590 1595 1600

Arg Asp Val Ala Gly Val Arg Ala Gly Glu Arg Val Leu Val His Ala

1605 1610 1615

Ala Ala Gly Gly Val Gly Met Ala Ala Val Arg Val Ala Arg Leu Leu

1620 1625 1630

Gly Ala Glu Val Tyr Ala Thr Ala Ser Pro Gly Lys His Glu Val Leu

1635 1640 1645

Arg Ala Ala Gly Leu Asp Glu Ala Arg Val Ala Ser Ser Arg Asp Thr

1650 1655 1660

Glu Phe Ala Gln Arg Phe Pro Glu Met Asp Val Val Leu Asn Ser Leu

1665 1670 1675 1680

Thr Gly Glu Phe Val Asp Ala Ser Leu Arg Leu Leu Arg Pro Gly Gly

1685 1690 1695

Arg Phe Val Glu Leu Gly Lys Thr Asp Arg Arg Asp Pro Ala Gly Leu

1700 1705 1710

Pro Gly Val Asp Tyr Leu Pro Phe Asp Leu Leu Leu Asp Ala Gly Pro

1715 1720 1725

Asp Arg Val Gln Ser Leu Leu Thr Glu Val Val Ala His Ala Glu Ala

1730 1735 1740

Gly Glu Leu Thr Gly Leu Pro Thr Arg Thr Trp Pro Leu Ala Asp Ala

1745 1750 1755 1760

Arg Thr Ala Phe Arg Phe Met Ala Gln Ala Arg His Thr Gly Lys Ile

1765 1770 1775

Val Leu Thr Val Ala Pro Tyr Ala Asp Gly Thr Val Leu Ile Thr Gly

1780 1785 1790

Ala Gly Val Leu Gly Gly Met Leu Ala Arg His Leu Val Ser Glu His

1795 1800 1805

Gly Ala Arg Asp Leu Val Leu Ala Ser Arg Arg Gly Ala Ala Ala Pro

1810 1815 1820

Gly Ser Ala Asp Leu Val Ala Glu Leu Ala Ala Ala Gly Ala Thr Val

1825 1830 1835 1840

Arg Phe Glu Thr Cys Asp Val Thr Asp Arg Ala Ala Leu Asp Ala Leu

1845 1850 1855

Leu Ala Lys Leu Thr Ala Glu Ala Pro Leu Thr Ala Val Val His Thr

1860 1865 1870

Ala Gly Ala Leu Asp Asp Gly Val Leu Thr Glu Leu Gly Ala Gly Arg

1875 1880 1885

Leu Pro Gly Val Leu Arg Pro Lys Ala Asp Ala Ala Trp His Leu His

1890 1895 1900

Glu Leu Thr Glu Asp Lys Asp Leu Ser Ala Phe Val Leu Phe Ser Ser

1905 1910 1915 1920

Ala Ala Ala Thr Leu Gly Thr Pro Ala Gln Ala Asn Tyr Ala Ala Ala

1925 1930 1935

Asn Ala Phe Leu Asp Ala Leu Ala Glu Arg Arg Arg Ala Ala Gly Leu

1940 1945 1950

Pro Gly Thr Ala Ala Ala Trp Gly Leu Trp Ala Asp Ala Thr Gly Leu

1955 1960 1965

Thr Arg His Leu Asp Ala Ala Asp Val Ala Arg Ala Gly Arg Asn Arg

1970 1975 1980

Ile Val Pro Met Ala Ala Ala Glu Gly Leu Ala Leu Phe Asp Thr Ala

1985 1990 1995 2000

Thr Ala Thr Gly Asp Ala Val Thr Val Thr Ala Arg Leu Asp Leu Ala

2005 2010 2015

Thr Ala Ser Ala Ala Pro Thr Pro Pro Leu Leu Arg Gly Leu Val Ala

2020 2025 2030

Thr Pro Ala Gly Pro Ala Ala Ala Arg Pro Val Pro Gly Ala Ala Ala

2035 2040 2045

Leu Val Ala Arg Ile Thr Gly Leu Pro Ala Pro Glu Arg Ala Pro Ala

2050 2055 2060

Leu Leu Asp Val Val Arg Gly Gln Val Ala Asp Val Leu Gly His Arg

2065 2070 2075 2080

Gly Arg Asp Ala Val Ala Pro Asp Arg Gly Phe Lys Glu Leu Gly Phe

2085 2090 2095

Asp Ser Leu Thr Ala Val Glu Leu Arg Asn Arg Leu Gly Thr Ala Thr

2100 2105 2110

Gly Leu Arg Leu Pro Thr Thr Ile Val Phe Asp His Pro Asn Pro Ala

2115 2120 2125

Ala Leu Ala Asp His Leu Leu Asp Ala Leu Leu Pro Ser Glu Ser Gly

2130 2135 2140

Glu Ala Ser Ala Asp Arg Ile Ile Ala Glu Leu Ala Arg Val Glu Ser

2145 2150 2155 2160

Ala Leu Gly Ala Leu Pro Ala Asp Gly Thr Asp Arg Ala Arg Val Ala

2165 2170 2175

Ala His Leu Arg Asp Leu Ala Ala Arg Trp Asp Ala Gly Thr Thr Asp

2180 2185 2190

Gly Thr Pro Glu Arg Ala Ala Leu Asp Gly Val Thr Ala Asp Glu Leu

2195 2200 2205

Phe Asp Leu Ile Asp Arg Gly Val Ser

2210 2215

<210>9

<211>4206

<212>PRT

<213>Streptomyces flaveolus

<400>1

Met Ala Asp Glu Ala Lys Leu Leu Asp Tyr Leu Lys Gln Val Thr Gly

1 5 10 15

Asp Leu Gln Val Ala Arg Arg Arg Leu Arg Glu Ala Glu Ala Arg Asp

20 25 30

Arg Glu Pro Ile Ala Ile Val Gly Met Ala Cys Arg Tyr Pro Gly Gly

35 40 45

Val Thr Ser Pro Glu Asp Leu Trp Arg Leu Val Thr Asp Gly Thr Asp

50 55 60

Ala Val Ser Ala Phe Pro Ala Asp Arg Gly Trp Asp Met Ala Ser Val

65 70 75 80

Tyr Asp Pro Asp Pro Asp Arg Pro Gly Thr Ser Tyr Ala Arg Glu Gly

85 90 95

Gly Phe Leu Asp Gly Ala Ala Asp Phe Asp Ala Gly Phe Phe Gly Ile

100 105 110

Ser Pro Arg Glu Ala Leu Ala Met Asp Pro Gln Gln Arg Leu Val Leu

115 120 125

Glu Thr Ser Trp Glu Ala Leu Glu Arg Ala Gly Ile Asp Pro Ala Gly

130 135 140

Leu Arg Ser Thr Ala Thr Gly Val Phe Val Gly Phe Ser Ser Glu Asp

145 150 155 160

Tyr Ser Asp Ile Thr Gly Pro Val Ala Thr Glu Leu Glu Gly Tyr Val

165 170 175

Val Thr Gly Thr Ser Pro Ser Val Leu Ser Gly Arg Val Ala Tyr Thr

180 185 190

Leu Gly Leu Glu Gly Pro Ala Leu Ser Val Asp Thr Ala Cys Ser Ser

195 200 205

Ser Leu Val Ala Leu His Leu Ala Val Arg Ser Leu Arg Ala Gly Glu

210 215 220

Cys Thr Leu Ala Leu Thr Gly Gly Ala Thr Val Leu Ser Thr Pro Gly

225 230 235 240

Val Phe Thr Glu Tyr Ser Arg Gln Arg Ala Leu Ala Ala Asp Gly Arg

245 250 255

Cys Lys Ala Phe Ala Ala Ala Ala Asp Gly Phe Gly Phe Ala Glu Gly

260 265 270

Ala Gly Met Leu Val Leu Glu Arg Leu Ser Asp Ala Arg Arg Asn Gly

275 280 285

His Pro Val Leu Ala Val Val Arg Gly Thr Ala Ile Asn Gln Asp Gly

290 295 300

Ala Ser Ser Gly Leu Thr Ala Pro Asn Gly Leu Ala Gln Gln Arg Val

305 310 315 320

Ile Arg Gln Ala Leu Ala Asp Ala Arg Leu Ala Ala Ser Gln Val Asp

325 330 335

Ala Val Glu Ala His Gly Thr Gly Thr Arg Leu Gly Asp Pro Ile Glu

340 345 350

Ala Gln Ala Leu Leu Ala Thr Tyr Gly Gln Glu Arg Asp Glu Pro Leu

355 360 365

Trp Leu Gly Ser Val Lys Ser Asn Ile Gly His Thr Gln Ala Ala Ala

370 375 380

Gly Val Ala Gly Val Ile Lys Met Val Gln Ala Met Arg His Gly Thr

385 390 395 400

Leu Pro Arg Thr Leu His Val Asp Glu Ala Ser Pro His Val Asp Trp

405 410 415

Thr Ala Gly Ala Val Glu Leu Leu Thr Glu Glu Arg Asp Trp Pro Gly

420 425 430

Gly Glu Gln Pro Arg Arg Ala Gly Val Ser Ser Phe Gly Val Ser Gly

435 440 445

Thr Asn Ala His Ala Ile Leu Glu Gln Ala Pro Ala Arg Pro Glu Pro

450 455 460

Thr Asp Glu Ala Thr Asp Arg Thr Leu Pro Val Val Pro Trp Thr Leu

465 470 475 480

Ser Ser Arg Thr Ala Ala Gly Leu Arg Ala Gln Ala Glu Arg Leu Leu

485 490 495

Ala His Arg Pro Ala His Asp Ala Ala Pro His Asp Val Ala Leu Ala

500 505 510

Leu Ala Thr Thr Arg Thr Ala Phe Glu His Arg Val Val Val Leu Gly

515 520 525

Ala Asp His Asp Thr Leu Leu Ala Gly Leu Thr Ala Val Ala Glu Gly

530 535 540

Ala Glu Ser Ala Asp Val Val Arg Gly Arg Ala Val Gly Asp Gly Arg

545 550 555 560

Ala Val Phe Val Phe Pro Gly Gln Gly Ala Gln Trp Val Gly Met Ala

565 570 575

Val Asp Leu Leu Asp Ser Ser Pro Val Phe Ala Gly Arg Met Ala Glu

580 585 590

Cys Ala Val Ala Leu Glu Pro Phe Val Gly Trp Ser Leu Arg Gly Val

595 600 605

Leu Gly Asp Pro Val Ala Leu Glu Arg Val Asp Val Val Gln Pro Val

610 615 620

Leu Trp Ala Val Met Val Ser Leu Ala Glu Val Trp Arg Ser Tyr Gly

625 630 635 640

Val Val Pro Ser Ala Val Val Gly His Ser Gln Gly Glu Ile Ala Ala

645 650 655

Ala Cys Val Ala Gly Val Leu Ser Leu Ala Asp Gly Ala Arg Val Val

660 665 670

Ala Leu Arg Ser Arg Ala Leu Thr Ala Leu Ala Gly Ser Gly Gly Met

675 680 685

Val Ser Val Ala Ala Gly Pro Ser Gly Val Glu Glu Leu Leu Val Gly

690 695 700

Trp Ala Gly Arg Leu Ala Val Ala Ala Val Asn Gly Pro Glu Ser Val

705 710 715 720

Val Val Ala Gly Glu Gly Val Ala Leu Glu Glu Phe Leu Ala His Cys

725 730 735

Gly Gly Arg Gly Val Arg Ala Arg Arg Ile Ala Val Asp Tyr Ala Ser

740 745 750

His Ser Val Leu Val Glu Pro Val Arg Glu Ala Leu Leu Ala Asp Leu

755 760 765

Glu Gly Val Arg Pro Gly Glu Gly Thr Val Pro Leu Phe Ser Thr Val

770 775 780

Thr Gly Glu Trp Ala Asp Gly Thr Ala Leu Asp Ala Gly Tyr Trp Tyr

785 790 795 800

Arg Asn Leu Arg Glu Pro Val Arg Tyr Ala Asp Ala Val Ala Ala Leu

805 810 815

Leu Ala Gln Gly His Arg Gly Phe Ile Glu Val Ser Pro His Pro Val

820 825 830

Leu Thr Val Gly Thr Gln Glu Thr Val Glu Arg Thr Glu Thr Gly Ala

835 840 845

Ala Val Val Gly Thr Leu Gln Arg Asp Arg Ala Gly Leu Pro Thr Leu

850 855 860

Leu Thr Asn Leu Ala Glu Ala His Thr His Gly Val Arg Ile Asp Trp

865 870 875 880

Thr Ala Phe Phe Arg Gly Thr Gly Ala His Pro Ala Asp Leu Pro Thr

885 890 895

Tyr Ala Phe Gln Arg Asp Arg Tyr Trp Pro Pro Val Asp Ala Ala Ala

900 905 910

Arg Pro Leu Pro Pro Ala Ala Thr Pro Val Ala Leu Pro Ala Gln Arg

915 920 925

Thr Ala Asp Glu Gly Thr Pro Trp Arg Asp Glu Leu Ala Gly Leu Thr

930 935 940

Pro Ala Glu Arg Arg His Arg Val Met Asp Leu Val Arg Leu Arg Thr

945 950 955 960

Ala Ala Val Leu Gly His Ala Asp Pro Ala Ala Ile Glu Pro His Arg

965 970 975

Gly Phe Ala Ala Leu Gly Phe Asp Ser Leu Ala Ser Leu Arg Leu Arg

980 985 990

Thr Ala Leu Thr Glu Ala Thr Gly Leu Thr Leu Ala Ser Ser Val Val

995 1000 1005

Phe Asp His Pro Asn Pro Ala Ala Leu Thr Asp His Leu Leu Thr Arg

1010 1015 1020

Leu Asp Gly Ala Pro Val Pro Ala Arg Pro Ala Val Arg Ala Thr Pro

1025 1030 1035 1040

Ala Asp Glu Pro Leu Ala Val Val Gly Met Ala Cys Arg Phe Pro Gly

1045 1050 1055

Gly Val Ala Ser Pro Asp Asp Leu Trp Gln Leu Leu Ala Glu Gly Arg

1060 1065 1070

Asp Val Ile Gly Asp Phe Pro Ala Asp Arg Gly Trp Asp Leu Asp Gly

1075 1080 1085

Leu Tyr Asp Pro Asp Pro Asp Arg Ala Gly His Thr Tyr Leu Arg Gln

1090 1095 1100

Gly Gly Phe Leu Asn Asp Ala Ala Gly Phe Asp Ala Asp Phe Phe Gly

1105 1110 1115 1120

Ile Ser Pro Arg Glu Ala Leu Ala Met Asp Pro Gln Gln Arg Leu Phe

1125 1130 1135

Leu Glu Val Ala Trp Glu Ala Phe Glu His Ala Gly Val Asp Pro His

1140 1145 1150

Gly Leu Arg Gly Ser Ala Thr Gly Val Phe Ala Gly Val Thr Asp Gln

1155 1160 1165

Arg Tyr Asp Ser Arg His Gly Ala Val Ala Gly Val Asp Glu Gly Leu

1170 1175 1180

Leu Gly Thr Gly Asn Tyr Ala Ser Val Leu Ser Gly Arg Val Ala Tyr

1185 1190 1195 1200

Thr Leu Gly Leu Glu Gly Pro Ala Val Ser Val Asp Thr Ala Cys Ser

1205 1210 1215

Ser Ser Leu Val Ala Leu His Leu Ala Gly Gln Ser Leu Arg Ser Gly

1220 1225 1230

Glu Cys Ser Leu Ala Leu Ala Gly Gly Val Met Val Met Ser Thr Pro

1235 1240 1245

Arg Ala Phe Val Glu Phe Ser Arg Gln Arg Gly Leu Ala Pro Asp Gly

1250 1255 1260

Arg Cys Lys Ala Phe Ala Ala Ala Ala Asp Gly Ile Gly Trp Ser Glu

1265 1270 1275 1280

Gly Ala Gly Val Val Val Leu Glu Arg Leu Ser Asp Ala Arg Arg Asn

1285 1290 1295

Gly His Pro Val Leu Ala Val Val Arg Gly Ser Ala Ile Asn Gln Asp

1300 1305 1310

Gly Ala Ser Ser Gly Leu Thr Ala Pro Asn Gly Ser Ala Gln Gln Arg

1315 1320 1325

Val Ile Arg Ser Ala Leu Ala Ala Ala Gly Leu Thr Ala Ala Asp Val

1330 1335 1340

Asp Val Val Glu Ala His Gly Thr Gly Thr Thr Leu Gly Asp Pro Ile

1345 1350 1355 1360

Glu Ala Asn Ala Leu Leu Ala Thr Tyr Gly Gln Asp Arg Ala Gln Pro

1365 1370 1375

Leu Arg Leu Gly Ser Leu Lys Ser Asn Ile Gly His Thr Gly Pro Ala

1380 1385 1390

Ala Gly Val Ala Gly Val Ile Lys Thr Val Leu Ala Leu Arg Ala Gly

1395 1400 1405

Glu Val Pro Arg Thr Leu His Val Asp Ala Pro Ser Pro His Ile Asp

1410 1415 1420

Trp Ala Ser Gly Ser Val Glu Leu Val Thr Gly Ala Ala Val Trp Pro

1425 1430 1435 1440

Pro Thr Asp Arg Pro Arg Arg Ala Gly Val Ser Ser Phe Gly Ala Ser

1445 1450 1455

Gly Thr Asn Ala His Leu Ile Leu Glu Glu Ala Pro Ala Glu Pro Ala

1460 1465 1470

Ala Pro Ala Arg Gly Ala Ala Pro Arg Trp Val Pro Trp Leu Leu Ser

1475 1480 1485

Ala Arg Ser Gln Ala Ala Leu Ala Glu Gln Ala Thr Arg Leu Ala Ala

1490 1495 1500

His Leu Asp Thr His Pro Gly Leu Asp Pro Val Asp Leu Ser Trp Ser

1505 1510 1515 1520

Leu Ala Ala Ser Arg Ala Leu Leu Pro His Arg Ala Val Val Val Ala

1525 1530 1535

Ala Asp Val Ala Gly Ala Arg Ala Ser Leu Ala Ala Leu Ala Ala Gly

1540 1545 1550

Glu Pro Val Glu Gly Val Val Ser Gly Val Ala Gly Leu Pro Gly Glu

1555 1560 1565

Gly Arg Val Val Phe Val Phe Pro Gly Gln Gly Ala Gln Trp Val Gly

1570 1575 1580

Met Ala Val Asp Leu Leu Asp Ser Ser Pro Val Phe Ala Gly Arg Met

1585 1590 1595 1600

Ala Glu Cys Ala Val Ala Leu Glu Pro Phe Val Gly Trp Ser Leu Arg

1605 1610 1615

Gly Val Leu Gly Asp Pro Val Ala Leu Glu Arg Val Asp Val Val Gln

1620 1625 1630

Pro Val Leu Trp Ala Val Met Val Ser Leu Ala Glu Val Trp Arg Ser

1635 1640 1645

Tyr Gly Val Val Pro Ser Ala Val Val Gly His Ser Gln Gly Glu Ile

1650 1655 1660

Ala Ala Ala Cys Val Ala Gly Val Leu Ser Leu Ala Asp Gly Ala Arg

1665 1670 1675 1680

Ile Val Ala Leu Arg Ser Arg Leu Ile Ala Glu Arg Leu Ala Gly Arg

1685 1690 1695

Gly Gly Met Val Ser Val Ala Leu Pro Glu Ala Thr Val Thr Arg Arg

1700 1705 1710

Leu Ala Pro Trp Ser Gly Arg Val Cys Val Ala Ala Leu Asn Gly Pro

1715 1720 1725

Ser Ser Val Val Leu Ser Gly Asp Pro Asp Ala Leu Asp Glu Val Met

1730 1735 1740

Ala Ala Trp Ser Ala Asp Gly Val Arg Leu Arg Arg Ile Ala Val Asp

1745 1750 1755 1760

Tyr Ala Ser His Ser Ala His Val Glu Ser Leu Glu Ala Glu Leu Arg

1765 1770 1775

Ala Ala Leu Ala Glu Leu Arg Pro Gly Glu Ala Gly Ile Ala Phe His

1780 1785 1790

Ser Thr Leu Leu Gly Gly Pro Leu Gly Glu Thr Arg Leu Asp Ala Gly

1795 1800 1805

Tyr Trp Tyr Arg Asn Leu Arg Glu Pro Val Arg Phe Glu Pro Val Val

1810 1815 1820

Arg Gly Leu Leu Asp Ser Gly His Ala Val Phe Val Glu Ile Ser Pro

1825 1830 1835 1840

His Pro Val Leu Thr Ala Ala Val Gln Glu Thr Ala Glu Ala Thr Glu

1845 1850 1855

Arg Thr Ala Val Val Val Gly Thr Leu Arg Arg Asp Glu Asp Gly Pro

1860 1865 1870

Arg Arg Leu Leu Thr Ser Leu Ala Glu Ala Ala Val His Gly Val Ser

1875 1880 1885

Val Asp Trp Thr Val Gly Cys Pro Asp Gly Arg His Val Asp Leu Pro

1890 1895 1900

Thr Tyr Ala Phe Gln Arg Arg Arg Phe Trp Pro Ser Gly Pro Val Ala

1905 1910 1915 1920

Ser Asp Ala Ala Gly Leu Gly Leu Thr Gly Ala Gly His Pro Leu Leu

1925 1930 1935

Gly Ala Val Thr Pro Leu Ala Gly Ser Gly Gly Gln Val Phe Thr Gly

1940 1945 1950

Arg Val Pro Ala Gly Thr Glu Leu Pro Ala Gly Ala Val Leu Asp Leu

1955 1960 1965

Ala Leu His Ala Ala Asp Gly Arg Thr Leu Gly Glu Leu Thr Glu Glu

1970 1975 1980

Ala Pro Leu Asp Thr Val Gly Glu Ala Arg Arg Leu Gln Val Thr Leu

1985 1990 1995 2000

Gly Ala Glu Asn Glu Asp Gly Ala Arg Pro Val Ala Val His Ser Arg

2005 2010 2015

Pro Ala Asp Ala Asp Asp Asp Gln Pro Trp Thr Arg His Ala Thr Gly

2020 2025 2030

Val Leu Leu Pro His Thr Gly Thr Ala Pro Ala Ala Ala Pro Gly Asp

2035 2040 2045

Pro Asp Thr Asp Val Thr Val Glu Leu Thr Asp Val Thr Val Glu Leu

2050 2055 2060

Thr Asp Glu Thr Ala Gly Gly Trp Gly Val His Pro Ala Leu Leu Thr

2065 2070 2075 2080

Asp Ala Leu Ala Ala Val His Pro Gly Leu Val Pro Gly Ala Trp His

2085 2090 2095

Gly Val Thr Leu His Ala Val Gly Ala Thr Arg Leu Arg Val Arg Leu

2100 2105 2110

His Pro Ala Gly Glu His Thr Val Thr Leu His Ala Thr Asp Asp Ser

2115 2120 2125

Gly Ala Ala Val Leu Thr Val Asp Ala Val Thr Leu Arg Pro Pro Ala

2130 2135 2140

Thr Ala Gly Pro Gly Arg Asp His Glu Leu Tyr Arg Val Glu Trp Thr

2145 2150 2155 2160

Pro Val Pro Leu Pro Ala Ala Asp Val Asp Gly Ile Ala Val Leu Gly

2165 2170 2175

Gly Leu Pro Leu Pro Gly Tyr Pro Ser Cys Pro Asp Leu Ala Ala Val

2180 2185 2190

Ala Ala Leu Asp Thr Val Pro Gly Thr Leu Val Leu Pro Cys Arg Gln

2195 2200 2205

Gln Ser Ala Asp Asp Thr Ala Gly Ala Ala His Ala Gly Ala Arg Arg

2210 2215 2220

Val Leu Thr Ala Leu Gln Glu Trp Leu Ala Asp Glu Arg Leu Ala Gly

2225 2230 2235 2240

Thr Arg Leu Ala Val Leu Thr His Gly Ala Val Pro Val Glu His Glu

2245 2250 2255

Asp Val Thr Asp Leu Ala His Ala Pro Val Trp Gly Leu Ile Arg Ser

2260 2265 2270

Ala Arg Ala Glu His Pro Gly Arg Leu Val Leu Val Asp Ile Asp Gly

2275 2280 2285

Pro Asp Ala Leu Gln Gln Leu Pro Ala Val Leu Ala Thr Gly Glu Pro

2290 2295 2300

Glu Ile Ala Val Arg Ser Gly Arg Val Leu Ala Pro Arg Leu Val Arg

2305 2310 2315 2320

Ala Pro Arg Thr Asp Glu Pro Thr Ser Gln Ala Ala Leu Trp Pro Ser

2325 2330 2335

Asp Gly Val Val Leu Val Thr Gly Gly Thr Gly Thr Leu Gly Ala Leu

2340 2345 2350

Cys Ala Arg His Leu Val Thr Glu His Gly Val Arg Arg Leu Val Leu

2355 2360 2365

Ala Gly Arg Arg Gly Asp Ala Ala Pro Asp Ala Val Thr Leu Ala Gly

2370 2375 2380

Glu Leu Arg Ala Leu Gly Ala Glu Val Thr Val Ala Ala Cys Asp Ala

2385 2390 2395 2400

Ala Asp Arg Thr Glu Leu Ala Ala Leu Leu Asp Arg Ile Thr Ala Glu

2405 2410 2415

His Pro Leu Thr Gly Val Val His Ala Ala Gly Val Leu Asp Asp Gly

2420 2425 2430

Val Ile Ala Ser Gln Thr Pro Glu Arg Leu Ala Lys Val Leu Arg Pro

2435 2440 2445

Lys Val Asp Ala Ala Trp Asn Leu His Glu Leu Thr Ala Pro His Arg

2450 2455 2460

Pro Ala Val Phe Val Leu Phe Ser Ser Thr Ser Gly Leu Phe Gly Ala

2465 2470 2475 2480

Pro Gly Gln Gly Asn Tyr Ala Ala Gly Asn Ala Phe Val Asp Ala Leu

2485 2490 2495

Ala Ala His Arg Arg Ala Gln Gly Leu Pro Ala Thr Ser Gln Val Trp

2500 2505 2510

Gly Leu Trp Ala His Ala Ser Gly Met Thr Gly His Leu Gly Gly Ala

2515 2520 2525

Asp Leu Arg Arg Ala Ala Arg Asp Gly Val Val Pro Leu Pro Thr Pro

2530 2535 2540

Asp Ala Leu Ala Leu Phe Asp Arg Ala Thr Ala Gly Ser Ala Pro Val

2545 2550 2555 2560

Val Val Pro Ala Trp Leu Asp Leu Thr Ser Phe Ala Thr Gly Val Thr

2565 2570 2575

Ala Val Pro Ala Leu Met Arg Arg Leu Val Arg Gly Pro Val Arg Arg

2580 2585 2590

Ala Ala Thr Ala Gly Ala Gly Pro Asp Thr Leu Ala Gly Lys Leu Thr

2595 2600 2605

Gly Leu Thr Ala Ala Glu Arg Glu Arg Thr Leu Leu Thr Leu Val Arg

2610 2615 2620

Ser His Ala Ala Val Val Leu Gly His Thr Asp Asp Thr Ala Val Thr

2625 2630 2635 2640

Pro Gly Arg Ala Phe Lys Glu Leu Gly Phe Asp Ser Leu Thr Ala Val

2645 2650 2655

Glu Leu Arg Asn Arg Leu Ser Ala Ala Thr Gly Leu Arg Leu Pro Ala

2660 2665 2670

Thr Leu Val Phe Asp His Pro Asn Pro Arg Ser Leu Ala Gly His Leu

2675 2680 2685

Leu Ala Glu Leu Leu Gly Glu Arg Ala Glu Glu Thr Ala Pro Val Pro

2690 2695 2700

Ala Val Ala Arg Pro Ala Asp Asp Asp Pro Ile Ala Ile Val Gly Met

2705 2710 2715 2720

Ala Cys Arg Tyr Pro Gly Gly Val Thr Ser Pro Glu Asp Leu Trp Arg

2725 2730 2735

Leu Leu Ala Asp Glu Arg Asp Ala Leu Thr Pro Phe Pro Asp Asp Arg

2740 2745 2750

Gly Trp Asp Leu Ala Gly Leu Phe His Pro Asp Pro Glu His Ala Gly

2755 2760 2765

Thr Ser Tyr Val Arg Glu Gly Gly Phe Leu Ala Asp Val Ala Gly Phe

2770 2775 2780

Asp Ala Asp Phe Phe Gly Ile Ser Pro Arg Glu Ala Leu Ala Met Asp

2785 2790 2795 2800

Pro Gln Gln Arg Leu Ala Leu Glu Thr Ala Trp Glu Ala Val Glu Arg

2805 2810 2815

Ala Gly Ile Asp Pro Lys Ser Leu Arg Gly Ala Asp Val Gly Val Tyr

2820 2825 2830

Leu Gly Thr Asn Gly Gln Asp Tyr Ala Gln Leu Val Arg Arg Thr Val

2835 2840 2845

Glu Ser Ala Glu Gly Tyr Val Gly Ile Gly Asn Ser Ala Ser Val Leu

2850 2855 2860

Ser Gly Arg Ile Ala Tyr Val Leu Gly Leu Glu Gly Pro Ala Val Thr

2865 2870 2875 2880

Val Asp Thr Ala Cys Ser Ala Ser Leu Val Ala Leu His Trp Ala Ile

2885 2890 2895

Gln Ala Leu Arg Ser Gly Glu Cys Ser Met Ala Leu Ala Gly Gly Val

2900 2905 2910

Thr Val Met Ser Ala Pro Asp Val Phe Val Asp Phe Ser Arg Gln Arg

2915 2920 2925

Gly Leu Ala Val Asp Gly Arg Cys Lys Ser Phe Ala Ala Ala Ala Asp

2930 2935 2940

Gly Thr Gly Trp Ser Glu Gly Ala Gly Met Leu Leu Val Glu Arg Leu

2945 2950 2955 2960

Ser Asp Ala Arg Arg His Gly His Gln Val Leu Ala Val Val Arg Gly

2965 2970 2975

Thr Ala Ile Asn Gln Asp Gly Ala Ser Asn Gly Leu Thr Ala Pro Asn

2980 2985 2990

Gly Pro Ser Gln Gln Arg Val Ile Arg Gln Ala Leu Ser Gly Ala Gly

2995 3000 3005

Leu Thr Pro Ala Asp Val Asp Ala Val Glu Ala His Gly Thr Gly Thr

3010 3015 3020

Arg Leu Gly Asp Pro Ile Glu Ala Gln Ala Leu Leu Ala Thr Tyr Gly

3025 3030 3035 3040

Gln Glu Arg Asp Glu Pro Leu Trp Leu Gly Ser Val Lys Ser Asn Ile

3045 3050 3055

Gly His Thr Gln Ala Ala Ala Gly Val Ala Gly Val Ile Lys Met Val

3060 3065 3070

Gln Ala Met Arg His Gly Thr Leu Pro Arg Thr Leu His Val Asp Glu

3075 3080 3085

Pro Ser Pro Glu Val Asp Trp Ala Ser Gly Ala Val Glu Leu Leu Thr

3090 3095 3100

Glu Ala Arg Glu Trp Ala Arg Ala Gly Arg Pro Arg Arg Ala Gly Val

3105 3110 3115 3120

Ser Ser Phe Gly Val Ser Gly Thr Asn Ala His Val Val Leu Glu Glu

3125 3130 3135

Ala Pro Glu Glu Thr Gly Arg Thr Ala Pro Ala Ala Leu Pro Ala Val

3140 3145 3150

Pro Trp Leu Leu Ser Ala Arg Ser Glu Thr Ala Leu Arg Gly Gln Val

3155 3160 3165

Glu Arg Leu Arg Ala Tyr Val Thr Glu His Pro Glu Ala Arg Pro Ala

3170 3175 3180

Asp Ile Gly Leu Ser Leu Leu Thr Ala Arg Ser Arg Phe Glu His Ser

3185 3190 3195 3200

Ala Val Val Val Gly Thr Asp His Asp Ala Leu Leu Ala Ala Leu Ala

3205 3210 3215

Asp Pro Glu Pro Leu His Ala Arg Asp Gly Leu Thr Ala Phe Val Phe

3220 3225 3230

Ala Gly Gln Gly Ala Gln Arg Val Gly Met Gly Ala Glu Leu Ala Ala

3235 3240 3245

Ala Tyr Pro Val Phe Ala Gln Val Phe Ala Gln Val Cys Ala Ala Phe

3250 3255 3260

Asp Gly Val Leu Glu Arg Pro Leu Gly Glu Val Val Ala Glu Gly Gly

3265 3270 3275 3280

Pro Glu Leu Asp Arg Thr Val Tyr Ala Gln Ala Gly Leu Phe Ala Phe

3285 3290 3295

Glu Val Ala Leu Phe Arg Leu Leu Glu Ser Trp Gly Val Ala Pro Asp

3300 3305 3310

Val Val Leu Gly His Ser Val Gly Glu Leu Thr Ala Ala Cys Val Ala

3315 3320 3325

Gly Val Trp Ser Leu Glu Asp Ala Val Arg Val Val Ala Ala Arg Gly

3330 3335 3340

Arg Leu Met Gln Ala Leu Pro Glu Gly Gly Ala Met Val Ala Leu Glu

3345 3350 3355 3360

Val Ser Ala Gly Glu Leu Glu Leu Pro Val Gly Val Glu Leu Ala Ala

3365 3370 3375

Val Asn Gly Pro Thr Ser Val Val Leu Ser Gly Glu Glu Glu Ala Val

3380 3385 3390

Leu Ala Glu Ala Ala Arg Trp Pro Asp Arg Arg Ala Lys Arg Leu Arg

3395 3400 3405

Val Ser His Ala Phe His Ser His Arg Met Asp Pro Met Leu Glu Asp

3410 3415 3420

Phe Arg Arg Val Leu Glu Ser Val Thr Phe Gln Ala Pro Glu Val Ala

3425 3430 3435 3440

Phe Val Ser Thr Val Thr Gly Ala Ala Val Thr Asp Glu Leu Cys Asp

3445 3450 3455

Pro Gly Tyr Trp Val Arg Asn Val Arg Glu Thr Val Arg Phe Ala Asp

3460 3465 3470

Ala Val Ala Ala Val Arg Ala Thr Gly Ala Asp Ala Leu Val Glu Ile

3475 3480 3485

Gly Pro Asp Ala Ala Leu Ala Pro Leu Ala Glu Gly Gly Val Pro Leu

3490 3495 3500

Leu Arg Arg Gly Arg Pro Glu Ala Val Ala Leu Val Glu Gly Leu Ala

3505 3510 3515 3520

Arg Ser Gln Met Ala Val Asp Trp Glu Lys Phe Phe Gly Ala Gly Val

3525 3530 3535

Ser Pro Val Asp Leu Pro Thr Tyr Pro Phe Gln His Ser Arg Phe Trp

3540 3545 3550

Pro Glu Val Pro Val Arg Ala Glu Ala Asp Pro Ala Ala Arg Trp Arg

3555 3560 3565

Tyr Arg Val Arg Trp Glu Pro Leu Arg Pro Ala Val Asp Ala Val Pro

3570 3575 3580

Ala Gly Arg Trp Leu Val Ala Val Pro Ala Ala Gly Ala Asp Ala Ala

3585 3590 3595 3600

Leu Val Arg Glu Cys Leu Lys Gly Leu Ala Gly Arg Gly Val Glu Val

3605 3610 3615

Ala Glu Leu Ala Val Ala Gly Ala Pro Asp Arg Ala Arg Leu Ala Glu

3620 3625 3630

Ala Val Ser Ala Thr Gly Pro Val Asp Gly Val Leu Ser Leu Leu Ala

3635 3640 3645

Ala Gly Pro Asp Ala Ala Ala Gly Thr Leu Val Leu Ala Gln Ala Leu

3650 3655 3660

Gly Asp Ala Gly Thr Asp Ala Pro Leu Trp Cys Val Thr Gly Gly Ala

3665 3670 3675 3680

Val Ala Ala Ala Asp Gly Glu Pro Ala Asp Pro Glu Gln Ala Gln Val

3685 3690 3695

Trp Gly Leu Gly Arg Val Leu Cys Leu Glu Ala Pro His Arg Trp Gly

3700 3705 3710

Gly Leu Val Asp Leu Pro Ala Glu Pro Asp Glu Gln Ala Val Val Gln

3715 3720 3725

Leu Cys Ala Val Leu Ala Gly His Asp Asp Glu Asp Gln Val Ala Val

3730 3735 3740

Arg Ala Ala Gly Val Phe Gly Arg Arg Leu Val Arg Pro Ala Thr Gly

3745 3750 3755 3760

Ala Ala Ala Glu Gly Trp Arg Pro Ser Gly Thr Val Leu Ile Thr Gly

3765 3770 3775

Gly Thr Gly Ala Leu Gly Gly His Thr Ala Arg Trp Ala Ala Arg Ser

3780 3785 3790

Gly Ala Gly Arg Val Val Leu Val Ser Arg Arg Gly Pro Asp Ala Asp

3795 3800 3805

Gly Ala Ala Glu Leu Val Ala Glu Leu Gly Glu Leu Gly Cys Gln Ala

3810 3815 3820

Val Ala Glu Ala Cys Asp Val Ala Asp Arg Ala Ala Leu Gly Ala Leu

3825 3830 3835 3840

Leu Asp Lys Ile Ala Ala Asp Gly Pro Pro Leu Thr Ala Val Val His

3845 3850 3855

Thr Ala Gly Val Leu Asp Asp Gly Val His Gly Ser Leu Thr Pro Glu

3860 3865 3870

Arg Leu Ala Thr Val Leu Arg Ala Lys Ala Asp Gly Ala Thr Ala Leu

3875 3880 3885

His Glu Leu Thr Ala His Leu Pro Leu Glu Ala Phe Val Leu Phe Ala

3890 3895 3900

Ala Leu Gly Gly Val Val Gly Gly Ala Gly Gln Ala Asn Tyr Ala Ala

3905 3910 3915 3920

Ala Asn Ala His Leu Asp Ala Leu Ala Ala Ser Arg Arg Ala Ala Gly

3925 3930 3935

Leu Pro Ala Thr Ala Val Ala Trp Gly Ala Trp Ala Gly Gly Gly Met

3940 3945 3950

Ala Asp Ala Asp Glu Val Arg Arg Arg Leu Asp Arg Asp Gly Leu Leu

3955 3960 3965

Pro Met Asp Pro Arg Arg Ala Leu Asp Ala Leu Gly Arg Glu Ile Ala

3970 3975 3980

Ala Gly Asp Pro Ala Val Val Leu Ala Asp Val Asp Trp Thr Arg Leu

3985 3990 3995 4000

Ala Pro Asn Leu His Ala Val Arg Pro Ser Pro Leu Ile Ser Thr Val

4005 4010 4015

Pro Glu Ala Arg Arg Ala Val Ala Pro Glu Pro Gly Thr Ala Gly Gly

4020 4025 4030

Glu Ser Asp Pro Arg Gln Arg Leu Ala Ala Leu Pro Asp Gly Glu Arg

4035 4040 4045

Ala Arg Val Leu Leu Asp Leu Val Arg Asp Ala Ile Ala Ala Val Leu

4050 4055 4060

Gly Tyr Gly Gly Pro Gly Ala Val Asp Ile Thr Arg Gly Leu Val Asp

4065 4070 4075 4080

Leu Gly Phe Asp Ser Leu Thr Ala Val Glu Leu Arg Asn Arg Leu Ala

4085 4090 4095

Arg Ala Thr Gly Leu Ala Leu Pro Leu Thr Leu Val Phe Asp His Pro

4100 4105 4110

Asp Gly Glu Ala Leu Ala Ala His Leu Ala Ala Ala Leu Ala Pro Ala

4115 4120 4125

Ala Asp Arg Pro Gln Thr Thr Asp Leu Asp Arg Phe Ala Arg Leu Asp

4130 4135 4140

Pro Ala Thr Ala Asp Glu Pro Thr Arg Val Arg Val Ala Ala Glu Leu

4145 4150 4155 4160

Arg Arg Leu Leu Asp Ala Trp Thr Pro Pro Pro Ala Ala Pro Ala Gly

4165 4170 4175

Ser Ala Gly Ser Ala Ala Asp Pro Ser Arg Leu Thr Thr Ala Ser Ala

4180 4185 4190

Asp Glu Ile Phe Asp Phe Ile Arg Asn Glu Leu Gly Lys Ser

4195 4200 4205

<210>11

<211>3628

<212>PRT

<213>Streptomyces flaveolus

<400>1

Val Glu Asn Glu Asn Lys Leu Leu Asp His Leu Arg Trp Val Thr Gly

1 5 10 15

Glu Leu Ala Gln Ala Arg Gln Thr Leu Arg Glu Thr Ala Glu Arg Ala

20 25 30

Thr Glu Pro Ile Ala Ile Val Gly Met Ala Cys Arg Phe Pro Gly Gly

35 40 45

Val Ser Thr Pro Glu Asp Leu Trp Arg Leu Leu Ala Asp Glu Arg Asp

50 55 60

Ala Val Gly Glu Phe Pro Ala Asp Arg Gly Trp Asp Leu Ala Ser Leu

65 70 75 80

His His Pro Asp Pro Glu His Ala Gly Thr Ser Tyr Val Arg Glu Gly

85 90 95

Gly Phe Leu Ala Asp Val Ala Gly Phe Asp Ala Asp Phe Phe Gly Ile

100 105 110

Ser Pro Arg Glu Ala Leu Ala Met Asp Pro Gln Gln Arg Leu Ala Leu

115 120 125

Glu Thr Ala Trp Glu Ala Val Glu Arg Ala Gly Leu Asp Pro Lys Ser

130 135 140

Leu Arg Gly Ala Asp Val Gly Val Tyr Leu Gly Thr Asn Gly Gln Asp

145 150 155 160

Tyr Ile Ser Ala Ala Arg Pro Leu Leu His Gln Val Glu Gly His Gly

165 170 175

Gly Thr Gly Ile Ala Gly Ser Val Leu Ser Gly Arg Val Ala Tyr Thr

180 185 190

Leu Gly Leu Arg Gly Pro Ala Ala Thr Val Asp Thr Ala Cys Ser Ala

195 200 205

Ser Leu Val Ala Leu His Trp Ala Met Arg Ala Leu Arg Gly Gly Glu

210 215 220

Cys Ala Met Ala Leu Ala Gly Gly Val Thr Val Met Ser Ser Pro Ala

225 230 235 240

Thr Phe Val Glu Phe Ser Arg Gln Arg Gly Leu Ala Ser Asp Gly Arg

245 250 255

Cys Lys Pro Phe Ala Ala Ala Ala Asp Gly Thr Gly Trp Gly Glu Gly

260 265 270

Ala Gly Met Leu Leu Leu Glu Arg Leu Ser Asp Ala Arg Arg Leu Gly

275 280 285

His Pro Val Leu Ala Val Leu Arg Gly Ser Ala Val Asn Gln Asp Gly

290 295 300

Ala Ser Ser Ala Leu Thr Ala Pro Asn Gly Pro Ala Gln Val Arg Val

305 310 315 320

Ile Gln Ala Ala Leu Ala Asp Ala Arg Leu Ala Pro Ala Asp Val Asp

325 330 335

Leu Leu Glu Ala His Gly Thr Gly Thr Val Leu Gly Asp Pro Ile Glu

340 345 350

Ala Gln Ala Leu Leu Thr Ala Tyr Gly Gln Asn Arg Thr Ala Pro Ala

355 360 365

Trp Leu Gly Ser Val Lys Ser Asn Val Gly His Thr Gln Ala Ala Ala

370 375 380

Gly Val Ala Gly Val Leu Lys Thr Val Leu Ala Leu Arg His Gly Val

385 390 395 400

Leu Pro Arg Thr Leu His Val Asp Ala Pro Thr Pro Lys Val Asp Trp

405 410 415

Ser Ala Gly Ala Val Arg Leu Leu Thr Glu Ala Arg Pro Trp Pro Ala

420 425 430

Gly Glu Arg Pro Arg Arg Ala Gly Val Ser Ala Phe Gly Val Ser Gly

435 440 445

Thr Asn Ala His Val Ile Val Glu Gln Ala Pro Glu Thr Ala Ala Glu

450 455 460

Ala Ser Gly Ala Ala Gly Gly Pro Val Pro Trp Val Leu Ser Gly Arg

465 470 475 480

Thr Glu Ser Ala Leu Arg Ala Gln Ala Ala Ala Leu Ala Ala His Leu

485 490 495

Ala Glu Arg Pro Gly Asp Arg Pro Gly Asp Val Ala Leu Ser Leu Ala

500 505 510

Thr Thr Arg Pro Ala Phe Glu His Arg Ala Val Val Val Gly Thr Asp

515 520 525

Val Asp Asp Leu Leu Arg Gly Val Ala Ala Val Ala Ala Gly Glu Pro

530 535 540

Thr Pro Gly Val Val Arg Gly Thr Ala Gly His Leu Gly Arg Ile Ala

545 550 555 560

Phe Val Phe Pro Gly Gln Gly Ser Gln Trp Thr Gly Met Ala Arg Glu

565 570 575

Leu Ala Asp Ala Ser Thr Glu Phe Ala Ala Arg Leu Asp Glu Cys Ala

580 585 590

Ala Ala Leu Ala Pro His Val Asp Trp Ser Leu Arg Asp Val Leu Ala

595 600 605

Asp Pro Ala Ala Leu Glu Arg Val Asp Val Val Gln Pro Ala Leu Trp

610 615 620

Ala Val Met Val Ser Leu Ala Ala Leu Trp Gln Ala His Gly Val Glu

625 630 635 640

Pro Ala Ala Val Val Gly His Ser Gln Gly Glu Ile Ala Ala Ala Cys

645 650 655

Val Ala Gly Ala Leu Ser Leu Glu Asp Ala Ala Leu Leu Val Thr Ala

660 665 670

Arg Ala Arg Ala Leu Arg Ala Leu Ile Gly Arg Gly Gly Met Val Ser

675 680 685

Leu Pro Leu Pro Glu Ala Ala Ala Arg Glu Val Ile Ala Ala Trp Gly

690 695 700

Asp Arg Leu Ser Val Ala Ala Val Asn Gly Pro Ala Ala Thr Val Val

705 710 715 720

Ser Gly Asp Ala Ala Gly Leu Asp Glu Leu Leu Ala Gln Ala Glu Arg

725 730 735

Asp Gly Leu Arg Ala Lys Arg Leu Pro Val Asp Tyr Ala Ser His Ser

740 745 750

Ala His Val Glu Ala Val Arg Ala Glu Val Leu Ala Ala Thr Ala Ala

755 760 765

Val Thr Pro Arg Ala Thr Gly Thr Ala Phe Val Ser Ser Val Thr Gly

770 775 780

Gly Phe Leu Asp Thr Thr Gly Leu Asp Ala Ala Tyr Trp Tyr Arg Asn

785 790 795 800

Leu Arg Glu Pro Val Arg Phe Asp Arg Ala Gly Arg Ala Leu Leu Asp

805 810 815

Ala Gly Phe Thr Thr Phe Ile Glu Val Ser Ala His Pro Val Leu Thr

820 825 830

Ala Ala Leu Gln Glu Ser Asp Pro Ala Val Leu Ala Val Gly Ser Leu

835 840 845

Arg Arg Asp Asp Gly Gly Pro Ala Arg Phe Leu Ala Ser Leu Ala Glu

850 855 860

Ala Ala Val Arg Gly Val Pro Val Asp Trp Arg Pro Ala Leu Thr Ala

865 870 875 880

Ala Arg Thr Val Pro Leu Pro Thr Tyr Ala Phe Gln Arg Glu Arg Leu

885 890 895

Trp Leu Glu Glu Asp Ala Gly Pro Ile Thr Pro Glu Arg Ala Arg Glu

900 905 910

Asp Ser Ala Phe Trp Ala Ala Val His Gly Ser Ala Asp Asp Leu Ala

915 920 925

Ala Val Leu Gly Ala Asp Pro Ala Ala Arg Glu Pro Ile Ala Ala Val

930 935 940

His Pro Leu Leu Ala Ala Trp His Glu Arg Arg Ala Asp Glu Ser Ala

945 950 955 960

Val Asp Ser Trp Arg Tyr Arg Val Asp Trp Thr Pro Leu Thr Ala Ser

965 970 975

Pro Ser Ala Gly Ala Pro Thr Gly Arg Trp Leu Ala Val Ala Ala Asp

980 985 990

Asp Pro Trp Ser Asp Ala Val Val Thr Ala Leu Ala Asp Arg Met Asp

995 1000 1005

Leu Thr Arg Val Pro Ala Tyr Asp Pro Gly Thr Val Ala Glu Leu Thr

1010 1015 1020

Ala Gly Thr Ala Gly Val Val Ala Val Leu Arg Pro Asp Asp Pro Asp

1025 1030 1035 1040

Pro Leu Pro Gly Leu Leu Ala Leu Leu Arg Ala His Asp Arg Ala Asp

1045 1050 1055

Gly Gly Leu Pro Leu Trp Cys Val Thr Arg Gly Ala Val Ala Thr Gly

1060 1065 1070

Pro Ala Asp Thr Pro Ala Asp Pro Ala Thr Ala Gln Ile Trp Gly Phe

1075 1080 1085

Gly Arg Val Ala Ala Leu Glu Arg Pro Gln Ser Trp Gly Gly Leu Ile

1090 1095 1100

Asp Leu Pro Ala Gly Ala Thr Val Thr Gly Asp Ala Leu Ala Gly Thr

1105 1110 1115 1120

Ala Arg Ala Asp Gly Pro Pro Asp Ala Arg Ile Ala Asp Asp Val Leu

1125 1130 1135

Gly Ala Arg Leu Ala Asp Arg Leu Val Ala Ala Leu Ala Gly Pro Glu

1140 1145 1150

Asp Gln Val Ala Val Arg Ala Ser Gly Ala Phe Gly Arg Arg Leu Arg

1155 1160 1165

Arg Ala Pro Ala Asp Gly Thr Thr Pro Asp Trp Arg Pro Thr Gly Thr

1170 1175 1180

Ile Leu Val Thr Gly Gly Thr Gly Gly Leu Gly Gly His Val Ala Arg

1185 1190 1195 1200

Trp Leu Ala Arg Ala Gly Ala Glu His Leu Val Leu Thr Gly Arg Arg

1205 1210 1215

Gly Pro Glu Ala Pro Gly Ala Ala Glu Leu Ala Ala Glu Leu Thr Ala

1220 1225 1230

Leu Gly Ala Glu Val Asp Ile Val Ala Cys Asp Ala Ala Asp Arg Asp

1235 1240 1245

Ala Leu Ala Arg Val Leu Ala Glu His Pro Val Asp Ala Val Phe His

1250 1255 1260

Leu Ala Gly Ile Glu Arg Tyr Arg Pro Leu Asp Glu Leu Thr Pro Ala

1265 1270 1275 1280

Asp Leu Thr Glu Val Ala Ala Ala Lys Val Thr Gly Ala Leu Leu Leu

1285 1290 1295

Asp Glu Leu Thr Arg Asp Arg Arg Leu Ser Ala Phe Val Leu Phe Thr

1300 1305 1310

Ser Gly Ala Gly Val Trp Gly Ser Ser Gly Gln Ala Ala Tyr Ala Ala

1315 1320 1325

Ala Asn Thr Arg Leu Asp Ala Leu Ala Ala Arg Arg Arg Ala Glu Gly

1330 1335 1340

Leu Pro Ala Thr Ala Val Ala Trp Gly His Trp Asp Gly Ala Gly Met

1345 1350 1355 1360

Ser Asp Gly Pro Ala Arg Ala Gln Met Leu Arg Trp Gly Leu Pro Ala

1365 1370 1375

Met Ala Pro His Arg Ala Val Ala Ala Leu Ala Gly Ala Leu Ala Arg

1380 1385 1390

Asp Glu Ala Ser Leu Val Val Ala Asp Ile Asp Trp Pro Val Phe Ala

1395 1400 1405

Pro Thr Phe Thr Leu Ala Arg Arg Ser Pro Leu Leu Ala Gly Leu Pro

1410 1415 1420

Glu Ala Thr Asp Pro Ala Asp Thr Ala Pro Pro Pro Ala Ala Pro Ala

1425 1430 1435 1440

Gly Arg Leu Asp Arg Ala Ala Leu Leu Glu Leu Val Leu Ala Glu Thr

1445 1450 1455

Gly Ala Val Leu Gly His Ala Ser Gly Thr Gly Leu Pro Ala Ala Arg

1460 1465 1470

Ala Phe Gln Glu Leu Gly Phe Asp Ser Leu Thr Ala Val Glu Leu Arg

1475 1480 1485

Asn Arg Leu Arg Asp Ala Thr Gly Leu Pro Leu Pro Ala Thr Leu Val

1490 1495 1500

Phe Asp His Pro Thr Pro Gln Ala Leu Ala Glu Arg Leu Ala Gly Glu

1505 1510 1515 1520

Leu Thr Gly Glu Gln Pro Ala Asp Val Pro Asp Thr Thr Pro Ala Pro

1525 1530 1535

Ala Ala Ala Ala Asp Asp Asp Pro Val Val Ile Val Gly Met Gly Cys

1540 1545 1550

Arg Phe Pro Gly Gly Ala Asp Ser Pro Glu Arg Leu Trp Asp Leu Val

1555 1560 1565

Ala Ala Gly Arg Asp Ala Met Thr Gly Phe Pro Ala Asp Arg Gly Trp

1570 1575 1580

Asp Val Thr Asp Thr Gly Tyr Thr Lys Ala Gly Gly Phe Leu Asp Ala

1585 1590 1595 1600

Ala Gly Glu Phe Asp Ala Gly Phe Phe Gly Ile Ser Pro Arg Glu Ala

1605 1610 1615

Thr Ser Met Asp Pro Gln Gln Arg Leu Leu Leu Glu Thr Ala Trp Glu

1620 1625 1630

Thr Val Glu Arg Ala Gly Ile Asp Pro Ala Ser Leu Arg Gly Ser Arg

1635 1640 1645

Thr Gly Val Phe Val Gly Tyr Gly Gly Gln Asp Tyr Leu Thr Ser Leu

1650 1655 1660

Tyr Gly Thr Pro Glu Glu Leu Gln Gly His Leu Leu Thr Gly Thr Ser

1665 1670 1675 1680

Gly Ser Val Val Ser Gly Arg Leu Ala Tyr Val Leu Gly Leu Glu Gly

1685 1690 1695

Pro Ala Val Thr Ile Asp Thr Ala Cys Ser Ser Ser Leu Val Ala Leu

1700 1705 1710

His Trp Ala Ile Arg Ala Leu Arg Ser Gly Glu Cys Asp Leu Ala Leu

1715 1720 1725

Ala Gly Gly Val Thr Val Met Ala Thr Pro Gly Val Phe Val Glu Phe

1730 1735 1740

Gly Arg Gln Gly Gly Leu Ala Gly Asp Gly Arg Cys Lys Ala Phe Ala

1745 1750 1755 1760

Asp Ala Ala Asp Gly Thr Gly Trp Gly Glu Gly Ala Gly Leu Leu Leu

1765 1770 1775

Val Glu Arg Leu Ser Asp Ala Arg Arg Asn Gly His Arg Val Leu Ala

1780 1785 1790

Val Val Arg Gly Ser Ala Val Asn Ser Asp Gly Ala Ser Asn Gly Leu

1795 1800 1805

Thr Ala Pro Asn Gly Pro Ser Gln Gln Arg Val Ile Arg Ala Ala Leu

1810 1815 1820

Ala Asp Gly Gly Leu Thr Pro Ala Asp Ile Asp Ala Val Glu Ala His

1825 1830 1835 1840

Gly Thr Gly Thr Ala Leu Gly Asp Pro Ile Glu Ala Gln Ala Leu Gln

1845 1850 1855

Val Val Tyr Gly Gln Asp Arg Glu Arg Pro Leu Trp Ile Gly Ser Val

1860 1865 1870

Lys Ser Asn Leu Gly His Thr Gln Ala Ala Ser Gly Ala Ala Gly Leu

1875 1880 1885

Ile Lys Thr Val Leu Ala Leu Arg His Gly Val Leu Pro Ala Thr Leu

1890 1895 1900

His Val Asp Arg Pro Ser Ala Gln Val Asp Trp Thr Arg Gly Ala Val

1905 1910 1915 1920

Ser Val Leu Thr Glu Ser Thr Pro Trp Pro Gly Thr Asp Val Pro Arg

1925 1930 1935

Arg Ala Ala Val Ser Ser Phe Gly Val Ser Gly Thr Asn Ala His Val

1940 1945 1950

Val Leu Glu Gln Ala Pro Pro Ala Gly Glu Ala Gly Pro Ala Gly Asp

1955 1960 1965

Gly Gly Pro Val Pro Trp Leu Val Ser Gly Arg Thr Pro Glu Ala Val

1970 1975 1980

Arg Ala Gln Val Glu Arg Leu Arg Ala Ser Leu Ala Gly His Pro Asp

1985 1990 1995 2000

Pro Val Ala Val Ala Arg Ala Leu Ala Thr Thr Arg Thr Ala Phe Glu

2005 2010 2015

Tyr Arg Val Ala Ala Ala Gly Gly Asp Thr Glu Ala Leu Leu Asp Ala

2020 2025 2030

Leu Ala Glu Ala Arg Pro Val Thr Ala Arg Gln Gly Arg Thr Ala Phe

2035 2040 2045

Leu Cys Thr Gly Gln Gly Ala Gln Arg Ala Gly Met Gly Ala Gly Leu

2050 2055 2060

Tyr Ala Ala His Pro Val Tyr Ala Asp Ala Phe Asp Ala Val Cys Ala

2065 2070 2075 2080

Glu Phe Asp Arg Leu Leu Asp Arg Pro Leu Arg Asp Leu Val Leu Ser

2085 2090 2095

Gly Pro Ala Asp Val Leu Asp Arg Thr Ala Tyr Ala Gln Pro Ala Leu

2100 2105 2110

Phe Ala Val Glu Ala Ala Leu Ala Ala Leu Leu Arg His Trp Gly Val

2115 2120 2125

Thr Pro Asp Leu Leu Ala Gly His Ser Leu Gly Glu Ile Thr Ala Ala

2130 2135 2140

His Leu Ala Gly Val Leu Ser Leu Pro Asp Ala Ala Ala Leu Val Ala

2145 2150 2155 2160

Ala Arg Gly Arg Leu Met Asp Ala Leu Pro Ala Gly Gly Ala Met Val

2165 2170 2175

Ala Val Glu Ala Asp Glu Asp Arg Val Gln Pro Leu Leu Gly Asp Asp

2180 2185 2190

Val Cys Leu Ala Ala Val Asn Gly Pro Arg Ala Leu Val Leu Ser Gly

2195 2200 2205

Arg Glu Glu Ala Val Asp Gly Val Ala Ala Arg Leu Ala Ala Glu Gly

2210 2215 2220

Cys Arg Thr Arg Arg Leu Arg Val Ser His Ala Phe His Ser Ala Leu

2225 2230 2235 2240

Met Glu Pro Met Leu Asp Glu Phe Arg Ala Thr Val Ala Ala Leu Asp

2245 2250 2255

Leu Arg Ala Pro Ser Val Pro Val Val Ser Ala Leu Thr Gly Arg Pro

2260 2265 2270

Leu Thr Ala Asp Glu Ala Arg Ser Pro Glu His Trp Val Arg His Val

2275 2280 2285

Arg Glu Ala Val Arg Phe His Asp Ala Val Arg Gly Leu Ala Ala Glu

2290 2295 2300

Gly Ala Val Arg Tyr Leu Glu Leu Gly Pro Asp Gly Val Leu Thr Ala

2305 2310 2315 2320

Leu Ala Gln Ser Ser Leu Pro Pro Ala Asp Thr Asp Ala Asp Gly Arg

2325 2330 2335

Asp Pro Leu Ala Val Pro Leu Leu Arg Ala Gly Arg Pro Glu Pro Glu

2340 2345 2350

Thr Leu Thr Asp Ala Leu Ala Arg Ala Ala Ala Asp Gly Leu Thr Val

2355 2360 2365

Asp Trp Ala Gly Tyr Phe Thr Gly Arg Gly Gly Ala Pro Val Glu Leu

2370 2375 2380

Pro Thr Tyr Ala Phe Gln Arg Glu His Tyr Trp Leu Pro Val Asp Ser

2385 2390 2395 2400

Gly Ala Gly Thr Ala Pro Ala Gly His Pro Leu Leu Ser Ala Ala Val

2405 2410 2415

Asp Leu Pro Asp Gly Gly Leu Val Leu Thr Gly Arg Leu Ser Pro Ala

2420 2425 2430

Ala Arg Pro Trp Leu Ala Gln His Thr Val Arg Gly Ser Ala Leu Leu

2435 2440 2445

Pro Gly Thr Ala Leu Leu Asp Leu Ala Leu Ala Ala Ala Gly Gln Ala

2450 2455 2460

Ala Ala Pro Gly Val Ala Glu Leu Ile Leu Glu Ala Pro Leu Val Leu

2465 2470 2475 2480

Pro Ala Glu Gly Ala Val Glu Val Arg Val Thr Val Gly Ala Ala Gly

2485 2490 2495

Thr Asp Gly Arg Arg Ala Ile Ala Leu His Thr Arg Ala Gly Asp Gly

2500 2505 2510

Asp Trp Thr Arg His Ala Thr Gly Ala Leu Gly Glu Val Pro Gly Glu

2515 2520 2525

Pro Thr Ala Ala Gly Ala Trp Pro Pro Pro Asp Ala Arg Pro Ala Asp

2530 2535 2540

Leu Ala Ala Leu Tyr Gly Gly Leu Ala Asp Ala Gly Phe Gly Tyr Gly

2545 2550 2555 2560

Pro Ala Tyr Gln Gly Leu Arg Ala Ala Trp Arg Arg Gly Glu Gly Pro

2565 2570 2575

Ala Ala Glu Val Phe Ala Glu Ala Glu Leu Pro Ala Ala Val Pro Asp

2580 2585 2590

Ala Asp Arg Cys Pro Val His Pro Ala Leu Leu Asp Ala Val Leu His

2595 2600 2605

Ala Ile Gly Val Gly Gly Leu Ile Thr Asp Pro Ala His Gly Gly Leu

2610 2615 2620

Pro Phe Ala Trp Thr Gly Val Arg Val Phe Ala Pro Gly Ala Arg Ala

2625 2630 2635 2640

Val Arg Ala Arg Leu Ser Arg Ala Gly Ala Glu Gly Ala Leu Ala Val

2645 2650 2655

Asp Leu Phe Asp Ala Asp Gly Leu Pro Val Ala Ala Ile Gly Ser Leu

2660 2665 2670

Arg Leu Arg Pro Pro Ala Ala Pro Ala Val Pro Asp Ala Leu Phe Glu

2675 2680 2685

Thr Ala Trp Thr Pro Val Glu Gln Gly Thr Ala Pro Ala Arg Arg Leu

2690 2695 2700

Ala Leu Leu Gly Ala Asp Thr Ala Leu Ala Ala Gly Leu Thr Ala Ala

2705 2710 2715 2720

Gly Ala Ala Leu Ala Asp Ala Thr Asp Arg Ser Ala Glu Val Leu Val

2725 2730 2735

Leu Pro Ile Val Thr Asp Pro Gly Ala Ala Pro Val Thr Glu Thr His

2740 2745 2750

Arg Ala Thr Ala Ala Val Leu Thr Ala Leu Arg Asp Val Leu Ala Asp

2755 2760 2765

Glu Glu Ser Thr Ala Arg Leu Ala Val Val Thr Arg Gly Ala Leu Ala

2770 2775 2780

Leu Ser Ala Glu Glu Ser Pro Asp Pro Ala Ala Arg Ala Val Trp Gly

2785 2790 2795 2800

Leu Val Arg Ser Ala Gln Thr Glu His Pro Asp Arg Ile Val Leu Ala

2805 2810 2815

Asp Leu Asp Ala Ala Asp Ala Ser Ala Arg Ala Leu Pro Ala Ala Leu

2820 2825 2830

Thr Cys Gly Glu Pro Gln Leu Ala Val Arg Ser Gly Ala Val Ser Ala

2835 2840 2845

Pro Arg Leu Thr Arg Ala Gly Ala Asp Ala Leu Val Leu Pro Asp Gly

2850 2855 2860

Gly Trp Arg Leu Arg Pro Gly Ala Thr Gly Thr Val Asp Gly Met Thr

2865 2870 2875 2880

Ala Val Pro His Pro Asp Ala Pro Leu Ala Asp Gly Glu Val Arg Val

2885 2890 2895

Ala Val Arg Ala Val Gly Val Thr Phe Arg Asp Val Leu Ser Val Leu

2900 2905 2910

Gly Leu Tyr Pro Gly Ala Pro Gln Pro Leu Gly Ile Glu Ala Ala Gly

2915 2920 2925

Val Val Thr Gly Thr Gly Pro Gly Val Ser Asp Leu Ala Pro Gly Asp

2930 2935 2940

Arg Val Phe Gly Leu Leu Pro Gly Ser Met Gly Ser Ser Ala Val Ala

2945 2950 2955 2960

Asp Arg Arg Val Leu Ala Pro Val Pro Asp Gly Trp Gly Phe Thr Arg

2965 2970 2975

Ala Ala Ser Val Pro Ser Ala Phe Leu Thr Ala Trp Phe Ala Leu Arg

2980 2985 2990

Asp Val Ala Gly Val Arg Ala Gly Glu Arg Val Leu Val His Ala Ala

2995 3000 3005

Ala Gly Gly Val Gly Met Ala Ala Val Arg Val Ala Arg Leu Leu Gly

3010 3015 3020

Ala Glu Val Tyr Ala Thr Ala Ser Pro Gly Lys His Gly Val Leu Arg

3025 3030 3035 3040

Ala Ala Gly Leu Asp Glu Ala Arg Val Ala Ser Ser Arg Asp Thr Glu

3045 3050 3055

Phe Ala Gln Arg Phe Pro Glu Met Asp Val Val Leu Asn Ser Leu Thr

3060 3065 3070

Gly Glu Phe Val Asp Ala Ser Leu Arg Leu Leu Arg Pro Gly Gly Arg

3075 3080 3085

Phe Val Glu Leu Gly Lys Thr Asp Leu Arg Thr Asp Thr Ala Gly Ile

3090 3095 3100

Thr Tyr Arg Ala Val Asp Leu Ala Asp Ala Gly Pro Asp Arg Ile Gln

3105 3110 3115 3120

Glu Met Leu Thr Glu Leu Leu Asp Arg Leu Ala Ala Gly Asp Leu Ala

3125 3130 3135

His Leu Pro Val Arg Ser Met Pro Met Gly Arg Ala Arg Glu Ala Phe

3140 3145 3150

Arg Phe Met Ala Gln Ala Arg His Thr Gly Lys Leu Val Leu Thr Thr

3155 3160 3165

Ala Pro Tyr Gly Asp Gly Thr Val Leu Val Thr Gly Gly Thr Gly Ala

3170 3175 3180

Leu Gly Gly Leu Val Ala Arg His Leu Val Thr Glu His Gly Ile Arg

3185 3190 3195 3200

Asp Leu Val Leu Val Gly Arg Gln Gly Ala Glu Pro Pro Val Thr Ala

3205 3210 3215

Glu Leu Arg Ala Ala Gly Ala Arg Val Arg Val Ala Ala Cys Asp Val

3220 3225 3230

Ser Asp Arg Ala Ala Leu Ala Ala Leu Leu Ala Asp Ile Glu Pro Pro

3235 3240 3245

Leu Thr Ala Val Val His Ala Ala Gly Val Leu Asp Asp Gly Thr Leu

3250 3255 3260

Thr Ser Leu Thr Pro Glu Arg Leu Ala Ala Val Leu Arg Pro Lys Ala

3265 3270 3275 3280

Asp Ala Ala Trp His Leu His Glu Leu Thr Glu Asp Arg Asp Leu Ser

3285 3290 3295

Ala Phe Val Leu Phe Ser Ser Ala Ala Gly Thr Phe Gly Ala Pro Gly

3300 3305 3310

Gln Gly Asn Tyr Ala Ala Ala Asn Ala Ala Leu Asp Ala Leu Ala Glu

3315 3320 3325

His Arg Arg Ser Arg Gly Leu Pro Ala Val Ser Leu Ala Trp Gly Pro

3330 3335 3340

Trp Ala Ala Glu Ser Ala Met Thr Gly Gly Leu Ser Ser Gly Asp Arg

3345 3350 3355 3360

Ala Arg Met Thr Arg Ala Gly Val Arg Pro Leu Ala Ala Thr Glu Ala

3365 3370 3375

Leu Ala Val Leu Asp Ala Ala Cys Arg Thr Gly Ala Gly Ala Leu Ala

3380 3385 3390

Ala Leu Arg Leu Asp Thr Ala Ala Leu Thr Ala Arg Thr Gly Ala Pro

3395 3400 3405

His Pro Leu Leu Arg Asp Leu Val Arg Arg Pro Ala Gly Pro Ala Arg

3410 3415 3420

Asp Asp Ala Asp Thr Gln Pro Ala Leu Pro Gln Arg Leu Ala Gly Leu

3425 3430 3435 3440

Gly Glu Glu Gln Arg Arg Arg Ala Val Leu Asp Val Val Arg Arg Asn

3445 3450 3455

Ala Ala Ala Val Leu Gly His Ala Arg Ala Ser Ala Val Asp Thr Ala

3460 3465 3470

Arg Gly Phe Leu Asp Leu Gly Phe Asp Ser Leu Thr Ala Val Glu Leu

3475 3480 3485

Arg Asn Arg Leu Thr Glu Ala Thr Gly Leu Arg Leu Ser Ala Thr Ala

3490 3495 3500

Val Phe Asp His Pro Thr Pro Ala Ala Leu Ala Arg His Leu Leu Thr

3505 3510 3515 3520

Glu Leu Glu Pro Leu Val Arg Ala Ala Arg Ser Ala Leu Pro Ser Ala

3525 3530 3535

Pro Asp Pro Asp Ala Asp Leu Arg Gly Ala Ile Ala Ala Ile Pro Leu

3540 3545 3550

Glu Arg Leu Arg Gln Ala Gly Leu Leu Asp Glu Leu Ala Arg Leu Ala

3555 3560 3565

Gly Val Ala Val Pro Ala Lys Asp Ala Pro Ala Glu Gln His Ala Ala

3570 3575 3580

Pro Asp Asp His Pro Asp Asp Pro Ala Asp Gly Pro Glu Asp Asp Gly

3585 3590 3595 3600

Ser Asp Asp Leu Met Asp Ala Leu Asp Asp Met Ser Ile Asp Asp Leu

3605 3610 3615

Ile Arg Ile Ala His Asp Glu Arg Pro Arg Gly Asn

3620 3625

<210>11

<211>8301

<212>PRT

<213>Streptomyces flaveolus

<400>1

Met Ser Asn Thr Asn Glu Glu Leu Val Glu Ala Leu Arg Ser Ser Leu

1 5 10 15

Arg Glu Thr Glu Arg Leu Arg Arg His Asn Arg Ser Leu Thr Ala Ala

20 25 30

Ala Asp Glu Pro Ile Ala Ile Val Gly Thr Ala Cys Arg Phe Pro Gly

35 40 45

Gly Ile Asp Ser Pro Glu Arg Leu Trp Asp Ala Val Ala Ala Gly Ser

50 55 60

Asp Leu Ile Thr Gly Phe Pro Asp Asp Arg Gly Trp Asp Leu Gly Val

65 70 75 80

His Asp Pro Asp Pro Glu Arg Ala Gly Arg Ser Tyr Thr Asp Arg Gly

85 90 95

Gly Phe Leu Thr Gly Ala Ala Asp Phe Asp Pro Ala Phe Phe Gly Ile

100 105 110

Ser Pro Arg Glu Ala Arg Ala Met Asp Pro Gln Gln Arg Val Leu Leu

115 120 125

Glu Thr Ala Trp Glu Ala Tyr Glu Gln Ala Gly Ile Asp Pro His Ala

130 135 140

Gln Arg Gly Ser Arg Thr Gly Val Phe Val Gly Thr Trp Ser Gln Gly

145 150 155 160

Tyr Gly Ile Gly Ala Arg Val Pro Glu Asp Ala Glu Gly Tyr Leu Val

165 170 175

Thr Gly Gly Ala Thr Ala Val Val Ser Gly Arg Ile Ser Tyr Val Leu

180 185 190

Gly Leu Glu Gly Pro Ala Val Thr Val Asp Thr Ala Cys Ser Ser Ser

195 200 205

Leu Val Ala Leu His Trp Ala Val Arg Ser Leu Arg Ala Gly Glu Cys

210 215 220

Ser Met Ala Leu Ala Gly Gly Val Thr Val Met Ala Gly Pro Gly Val

225 230 235 240

Phe Val Glu Phe Ser Arg Glu Arg Gly Leu Ala Pro Asp Gly Arg Cys

245 250 255

Lys Ala Tyr Ser Ala Asp Ala Asp Gly Thr Gly Trp Gly Glu Gly Val

260 265 270

Gly Val Leu Leu Leu Glu Arg Leu Ser Asp Ala Val Arg Asn Gly His

275 280 285

Arg Val Leu Ala Val Val Arg Gly Ser Ala Val Asn Ser Asp Gly Ala

290 295 300

Ser Ser Gly Leu Thr Ala Pro Asn Gly Pro Ser Gln Gln Glu Val Ile

305 310 315 320

Arg Gln Ala Leu Ala Asp Ala Arg Leu Thr Pro Ser Asp Val Asp Val

325 330 335

Val Glu Gly His Gly Thr Gly Thr Arg Leu Gly Asp Pro Ile Glu Ala

340 345 350

Gln Ala Leu Leu Ala Ala Tyr Gly Gln Asp Arg Pro Arg Pro Leu Leu

355 360 365

Leu Gly Ser Val Lys Ser Asn Leu Gly His Thr Gln Ala Ala Ala Gly

370 375 380

Val Ala Gly Val Ile Lys Met Val Glu Ala Met Arg Arg Gly Ile Ala

385 390 395 400

Pro Arg Thr Leu His Ala Thr Glu Pro Thr Pro Gln Val Asp Trp Ser

405 410 415

Arg Gly Ala Val Glu Leu Leu Thr Asp Asn Arg Ser Trp Pro Asp Thr

420 425 430

Gly Ala Pro Arg Arg Ser Ala Val Ser Ala Phe Gly Val Ser Gly Thr

435 440 445

Asn Ala His Ile Val Leu Glu Gln Ala Pro Glu Pro Ala Asp Thr Asp

450 455 460

Thr Thr Pro Arg Thr Ala His Pro Val Val Pro Trp Leu Leu Ser Ala

465 470 475 480

His Thr Pro Ala Ala Leu Arg Ala Gln Ala Glu Arg Leu Ser Ala Gly

485 490 495

Leu Pro Asp Asp Ala Asp Pro Leu Asp Val Ala Ala Ala Leu Ala Thr

500 505 510

Thr Arg Ala Ala Leu Pro Leu Arg Ala Ala Val Leu Gly Ala Asp Ala

515 520 525

Thr Gln Leu Arg Ala Gly Leu Ala Ala Leu Ala Ala Gly Ala Pro Ala

530 535 540

Ala Leu Ser Gly Glu Ala Arg Ser Asp Arg Thr Thr Ala Phe Leu Tyr

545 550 555 560

Thr Gly Gln Gly Ala Gln Arg Ala Gly Met Gly Glu Glu Leu Ala Ala

565 570 575

Ala Tyr Pro Ala Phe Ala Ala Ala Trp Thr Glu Val Cys Ala Glu Phe

580 585 590

Asp Thr Val Leu Pro Arg Pro Leu Arg Gln Val Ile Thr Glu Gly Gly

595 600 605

Pro Asp Leu Asp Arg Thr Leu Tyr Ala Gln Ala Ala Val Phe Ala Phe

610 615 620

Glu Thr Ala Leu Thr Ala Leu Leu Gly Ser Trp Gly Ile Arg Pro Asp

625 630 635 640

Leu Val Leu Gly His Ser Val Gly Glu Leu Ala Ala Ala His Thr Ala

645 650 655

Gly Val Leu Ser Leu Arg His Ala Val Val Val Val Ala Ala Arg Gly

660 665 670

Arg Leu Met Glu Ala Leu Pro Glu Gly Gly Ala Met Val Ala Val Gln

675 680 685

Ala Ser Glu Asp Glu Ile Glu Leu Pro Glu Gly Val Ala Leu Ala Ala

690 695 700

Val Asn Gly Pro Ser Ser Val Val Leu Ser Gly Asp Glu Ala Ala Val

705 710 715 720

Leu Ala Thr Ala Ala His Trp Ala Glu Arg Gly Cys Arg Thr Lys Arg

725 730 735

Leu Thr Val Ser His Ala Phe His Ser His Arg Met Asp Pro Val Leu

740 745 750

Asn Gly Phe Arg Arg Val Leu Gly Ala Val Thr Leu Asn Thr Pro Arg

755 760 765

Ile Pro Phe Val Ser Thr Val Thr Gly Ala Pro Val Glu Ala Gly Leu

770 775 780

Arg Asp Pro Glu Tyr Trp Leu Arg Asn Val Arg Asp Thr Val Arg Phe

785 790 795 800

Ala Asp Gly Val Arg Thr Leu Ala Asp Glu Gly Ala Asp Thr Phe Val

805 810 815

Glu Val Gly Pro Asp Ala Val Leu Gly Ala Leu Val Ala Asp Ala Leu

820 825 830

Pro Asp Asp Pro Asp Arg Pro Glu Ile Leu Ala Val Pro Thr Ala Arg

835 840 845

Ala Asp Arg Pro Glu Pro Glu Thr Leu Val Gly Ala Leu Ala Arg Ile

850 855 860

His Ala His Gly Ala His Val Asp Trp Ala Ala Phe Phe Gly Ser Gly

865 870 875 880

Ala Arg Arg Val Asp Leu Pro Thr Tyr Ala Phe Gln His Gln His Tyr

885 890 895

Trp Leu Ala Pro Ala Pro Ala Asp Asp Leu Pro Ala Ala Gly Leu Gly

900 905 910

Thr Val Gly His Pro Leu Leu Arg Ala Ala Val Glu Leu Pro Ala Asp

915 920 925

Ala Thr Ala Asp His Ala Ala Arg Pro Gly Ala Ala Gly Ala Val Val

930 935 940

Phe Thr Gly Thr Leu Ser Ala His Thr His Pro Trp Leu Ala Asp His

945 950 955 960

Ser Val Leu Gly Thr Pro Val Leu Pro Gly Thr Ala Leu Ala Glu Leu

965 970 975

Ala Ala Ala Ala Gly Asp Arg Leu Gly Cys Ala Thr Val Ala Glu Leu

980 985 990

Val Leu Thr Ala Pro Leu Ala Val Pro Ala Ser Gly Ala Val Arg Leu

995 1000 1005

Arg Val His Val Asp Ala Pro Asp Arg Asp Gly His Arg Ala Val Thr

1010 1015 1020

Val Asp Ser Arg Pro Asp Asp Gln Leu Ala Asp Gly Ala Ala Trp Thr

1025 1030 1035 1040

Arg His Ala Thr Gly Arg Leu Ala Pro Thr Gly Pro Glu Pro Ala Gln

1045 1050 1055

Ala Pro Thr Ala Trp Pro Pro Ala Asp Ala Glu Pro Leu Pro Val Asp

1060 1065 1070

Gly Leu Tyr Asp Arg Leu Thr Ala Ala Gly Phe Gly Tyr Gly Pro Ala

1075 1080 1085

Phe Arg Gly Val Arg Ala Ala Trp Arg Leu Gly Thr Asp Leu Leu Ala

1090 1095 1100

Asp Val Glu Leu Pro Ala Asp Thr Asp Glu Ser Gly Phe Leu Leu His

1105 1110 1115 1120

Pro Ala Leu Phe Asp Ala Ala Leu His Ala Leu Gly Leu Gly Gly Leu

1125 1130 1135

Val Glu His Gly Gly Leu Pro Phe Thr Trp Thr Gly Val Arg Leu His

1140 1145 1150

Ala Thr Gly Ala Arg Ser Leu Arg Val Arg Leu Thr Pro Thr Gly Pro

1155 1160 1165

Asp Ala Val Ala Leu Thr Ala Ala Asp Ala Thr Gly Arg Pro Val Val

1170 1175 1180

Thr Val Thr Asp Leu Arg Leu Arg Pro Ala Thr Glu Val Arg Gly Thr

1185 1190 1195 1200

Thr Thr Ala Asp Ala Leu His His Ile Arg Trp Glu Pro Arg Pro Ala

1205 1210 1215

Ala Ala Ala Thr Asp Val Thr Gly Thr Asp Phe Thr Val Phe Thr Leu

1220 1225 1230

Pro Ala Ala Gly Ser Asp Pro Gln Ala Val Arg Glu Ala Thr Arg Ala

1235 1240 1245

Ser Leu Ala Arg Leu Gln Glu His Leu Ala Ala Val Gly Pro Ala Gly

1250 1255 1260

Pro Leu Val Val Val Thr His Gly Ala Val Ala Ala Glu Pro Gly Asp

1265 1270 1275 1280

Thr Val Pro Asp Leu Ala Gly Ala Ala Val Trp Gly Leu Val Arg Ser

1285 1290 1295

Ala Gln Ser Glu His Pro Gly Arg Phe Val Leu Ile Asp Leu Ala Ala

1300 1305 1310

Ser Glu Asp Ala Ala Val Val Pro Ala Ala Ile Ala Thr Gly Glu Pro

1315 1320 1325

Gln Ile Ala Val Arg Asn Gly Ala Leu Tyr Ala Pro Arg Leu Val Arg

1330 1335 1340

Gly Ala Ser Arg Pro Gly Gly Glu Val Pro Phe Gly Ala Gly Asp Val

1345 1350 1355 1360

Val Leu Val Thr Gly Gly Thr Gly Ala Leu Gly Arg Leu Val Ala Arg

1365 1370 1375

His Leu Val Ala Glu His Gly Val Arg Arg Leu Val Leu Ala Ser Arg

1380 1385 1390

Arg Gly Gly Ala Gly Glu Leu Val Ala Glu Leu Gly Glu Leu Gly Ala

1395 1400 1405

Ala Val Asp Val Val Ala Cys Asp Val Ser Asp Arg Asp Ala Leu Glu

1410 1415 1420

Arg Leu Val Ala Ala His Pro Leu Thr Gly Val Val His Ala Ala Gly

1425 1430 1435 1440

Val Leu Asp Asp Gly Thr Val Glu Ser Leu Thr Pro Asp Arg Val Asp

1445 1450 1455

Gly Val Leu Arg Ala Lys Val Asp Gly Ala Trp His Leu His Glu Leu

1460 1465 1470

Thr Ala Ala Leu Asp Leu Arg Ala Phe Val Leu Phe Ser Ser Leu Ala

1475 1480 1485

Gly Val Val Gly Ser Ala Gly Gln Gly Gly Tyr Ala Ala Ala Asn Ala

1490 1495 1500

Ala Leu Asp Ala Leu Ala Glu His Arg Arg Ala Glu Gly Leu Pro Ala

1505 1510 1515 1520

Thr Ser Ile Ala Trp Gly Pro Trp Thr Asp Gly Met Ala Ser Gly Leu

1525 1530 1535

Asp Arg Val Asp Ala Ala Arg Leu Ala Arg Gly Gly Val Val Pro Phe

1540 1545 1550

Ala His Ala Asp Gly Leu Glu Leu Phe Asp Ala Val Cys Ala Thr Asp

1555 1560 1565

Ala Pro Leu Thr Val Ala Ala Arg Leu Asp Leu Ala Val Leu Arg Ala

1570 1575 1580

Gln Ala Glu Ala Leu Pro Pro Val Leu Arg Gly Leu Val Pro Ala Pro

1585 1590 1595 1600

Ala Arg Arg Thr Ala Ala Ala Ala Arg Pro Gly Gly Phe Ala Glu Arg

1605 1610 1615

Leu Ala Ala Leu Pro Glu Thr Glu Arg Arg Arg Ala Ala Leu Glu Leu

1620 1625 1630

Val Arg Glu Thr Ala Ala Thr Val Leu Gly His Val Gly Pro Glu Ala

1635 1640 1645

Val Ala Pro Asp Arg Ser Phe Leu Asp Leu Gly Phe Asp Ser Leu Ala

1650 1655 1660

Ala Val Glu Leu Arg Asn Arg Leu Thr Ala Ala Thr Gly Leu Arg Leu

1665 1670 1675 1680

Ala Ala Thr Ile Thr Phe Asp His Pro Thr Ser Ala Ala Leu Ala Arg

1685 1690 1695

His Leu Leu Asp Ala Ala Leu Asp Ala Gly Pro Ser Ala Thr Ser Gly

1700 1705 1710

Ala His Leu Pro Ala Ala Thr Ala Val Arg Ala Asp Glu Pro Ile Ala

1715 1720 1725

Ile Val Gly Met Ala Cys Arg Tyr Pro Gly Gly Val Thr Ser Pro Glu

1730 1735 1740

Asp Leu Trp Arg Leu Val Leu Ala Gly Ala Asp Ala Val Thr Gly Phe

1745 1750 1755 1760

Pro Glu Asp Arg Gly Trp Asp Leu Thr Ser Val Tyr Asp Pro Asp Gln

1765 1770 1775

Ser Arg Thr Gly Thr Ser Tyr Thr Arg Glu Gly Gly Phe Leu Thr Gly

1780 1785 1790

Ala Ala Asp Phe Asp Ala Glu Phe Phe Gly Ile Ser Pro Arg Glu Ala

1795 1800 1805

Leu Ala Met Asp Pro Gln Gln Arg Leu Leu Leu Glu Thr Ser Trp Glu

1810 1815 1820

Ala Ile Glu Arg Ala Gly Ile Asp Pro Thr Ala Leu Arg Gly Ser Ala

1825 1830 1835 1840

Thr Gly Val Phe Ala Gly Leu Met Tyr His Asp Tyr Gly Ser Gly Thr

1845 1850 1855

Gly Thr Leu Pro Glu Gly Val Glu Gly Tyr Leu Gly Leu Gly Thr Ala

1860 1865 1870

Gly Ser Val Leu Ser Gly Arg Val Ala Tyr Thr Leu Gly Leu Glu Gly

1875 1880 1885

Pro Ala Val Thr Val Asp Thr Ala Cys Ser Ser Ser Leu Val Ala Leu

1890 1895 1900

His Trp Ala Ile Gln Ala Leu Arg Ser Gly Glu Cys Ser Met Ala Leu

1905 1910 1915 1920

Ala Gly Gly Val Thr Val Met Ala Thr Pro Gly Thr Phe Val Glu Phe

1925 1930 1935

Ser Arg Gln Arg Gly Leu Ala Pro Asp Gly Arg Cys Lys Ser Phe Ala

1940 1945 1950

Ala Ala Ala Asp Gly Val Gly Trp Ser Glu Gly Val Gly Met Leu Leu

1955 1960 1965

Val Glu Arg Leu Ser Asp Ala Arg Arg Asn Gly His Gln Val Leu Ala

1970 1975 1980

Val Val Arg Gly Ser Ala Val Asn Gln Asp Gly Ala Ser Asn Gly Leu

1985 1990 1995 2000

Thr Ala Pro Asn Gly Pro Ser Gln Gln Arg Val Ile Arg Gln Ala Leu

2005 2010 2015

Gln Gly Ala Gly Leu Thr Thr Ala Asp Val Asp Ala Val Glu Ala His

2020 2025 2030

Gly Thr Gly Thr Lys Leu Gly Asp Pro Ile Glu Ala Gln Ala Leu Leu

2035 2040 2045

Ala Thr Tyr Gly Gln Glu Arg Asp Glu Pro Leu Trp Leu Gly Ser Val

2050 2055 2060

Lys Ser Asn Ile Gly His Thr Gln Ala Ala Ala Gly Val Ala Gly Val

2065 2070 2075 2080

Ile Lys Met Val Gln Ala Met Arg His Gly Thr Leu Pro Arg Thr Leu

2085 2090 2095

His Val Asp Glu Ala Ser Pro His Val Asp Trp Thr Ala Gly Ala Val

2100 2105 2110

Glu Leu Leu Thr Glu Glu Arg Glu Trp Thr Arg Thr Arg Arg Pro Arg

2115 2120 2125

Arg Ala Ala Val Ser Ser Phe Gly Val Ser Gly Thr Asn Ala His Val

2130 2135 2140

Val Leu Glu Glu Ala Pro Glu Glu Thr Ala Pro Pro Val Ser Ala Glu

2145 2150 2155 2160

Leu Pro Leu Val Pro Leu Leu Leu Ser Gly His Thr Ser Thr Ala Leu

2165 2170 2175

Ala Ala Gln Ala Arg Arg Leu His Asp His Leu Ala Glu Ser Gly Thr

2180 2185 2190

Pro Ala Leu Glu Thr Val Gly Arg Ser Leu Ala Ala Ser Arg Ala Leu

2195 2200 2205

Leu Pro His Arg Ala Val Val Val Ala Ala Asp Val Ala Gly Ala Arg

2210 2215 2220

Ala Ser Leu Ala Ala Leu Ala Ala Gly Glu Pro Val Glu Gly Val Val

2225 2230 2235 2240

Ser Gly Ala Ala Gly Leu Pro Gly Glu Gly Arg Val Val Phe Val Phe

2245 2250 2255

Pro Gly Gln Gly Ala Gln Trp Val Gly Met Ala Val Asp Leu Leu Asp

2260 2265 2270

Ser Ser Pro Val Phe Ala Gly Arg Met Ala Glu Cys Ala Val Ala Leu

2275 2280 2285

Glu Pro Phe Val Glu Trp Ser Leu Arg Gly Val Leu Gly Asp Pro Val

2290 2295 2300

Ala Leu Glu Arg Val Asp Val Val Gln Pro Val Leu Trp Ala Val Met

2305 2310 2315 2320

Val Ser Leu Ala Glu Val Trp Arg Ser Tyr Gly Val Val Pro Ser Ala

2325 2330 2335

Val Val Gly His Ser Gln Gly Glu Ile Ala Ala Ala Cys Val Ala Gly

2340 2345 2350

Val Leu Ser Leu Ala Asp Gly Ala Arg Val Val Ala Leu Arg Ser Arg

2355 2360 2365

Ala Leu Thr Ala Leu Ala Gly Ser Gly Gly Met Val Ser Val Ala Ala

2370 2375 2380

Gly Pro Ser Gly Val Glu Glu Leu Leu Val Gly Trp Ala Gly Arg Leu

2385 2390 2395 2400

Ala Val Ala Ala Val Asn Gly Pro Glu Ser Val Val Val Ala Gly Glu

2405 2410 2415

Gly Val Ala Leu Glu Glu Phe Leu Ala His Cys Gly Gly Arg Gly Val

2420 2425 2430

Arg Ala Arg Arg Ile Ala Val Asp Tyr Ala Ser His Ser Val Leu Val

2435 2440 2445

Glu Pro Val Arg Glu Ala Leu Leu Ala Asp Leu Glu Gly Val Arg Pro

2450 2455 2460

Gly Glu Gly Thr Val Pro Leu Phe Ser Thr Val Thr Gly Glu Trp Ala

2465 2470 2475 2480

Asp Gly Thr Ala Leu Asp Ala Gly Tyr Trp Tyr Arg Asn Leu Arg Glu

2485 2490 2495

Pro Val Gly Phe Glu Pro Ala Val Arg Gly Leu Leu Asp Ser Gly His

2500 2505 2510

Ala Val Phe Val Glu Ile Ser Pro His Pro Val Leu Thr Ala Ala Val

2515 2520 2525

Gln Glu Thr Ala Asp Ala Thr Glu Arg Thr Ala Val Val Val Gly Thr

2530 2535 2540

Leu Arg Arg Asp His Glu Gly Gln Arg Gln Leu Leu Thr His Leu Gly

2545 2550 2555 2560

Val Leu His Thr Thr Gly Ala Asp Ile Asp Trp Thr Gly Cys Phe Thr

2565 2570 2575

Gly Val Thr Gly Arg Ala Asp Leu Pro Thr Tyr Ala Phe Gln His Thr

2580 2585 2590

Arg Tyr Trp Leu Thr Pro Ser Gly Pro Ser Ala Gly Glu Leu Ala Gly

2595 2600 2605

Ala Gly Leu Thr Ala Ala Gly His Pro Leu Leu Gly Ala Ala Val Asp

2610 2615 2620

Leu Ala Glu Asp Gly Gly Leu Val Leu Thr Gly Arg Leu Ala Ala Asp

2625 2630 2635 2640

Pro Ala Ala Trp Thr Ala Asp His Val Val Leu Gly Thr Thr Leu Leu

2645 2650 2655

Pro Gly Ala Ala Leu Ala Glu Leu Ala Leu Ala Ala Gly Asp Gly Val

2660 2665 2670

Gly Cys Gly Thr Leu Asp Glu Leu Val Leu Gly Ala Pro Leu Ala Leu

2675 2680 2685

Pro Glu Arg Gly Ala Leu His Leu Gln Val Arg Val Gly Arg Ala Glu

2690 2695 2700

Ala Asp His Arg Arg Thr Val Ser Val His Ala Arg Pro Glu Asp Gly

2705 2710 2715 2720

Asp Ala Pro Trp Thr Arg His Ala Glu Gly Val Leu Val Pro Gly Asp

2725 2730 2735

Thr Ala Ala Gly Thr Pro Leu Thr Glu Trp Pro Pro Ala Asp Ala Glu

2740 2745 2750

Pro Val Asp Val Ser Ala Leu Tyr Asp Ser Leu Ala Asp Arg Gly Leu

2755 2760 2765

Asp Tyr Gly Pro Val Phe Arg Gly Val Arg Ala Ala Trp Arg His Gly

2770 2775 2780

Asp Asp Ile Leu Ala Glu Val Glu Leu Pro Ala Glu Ala Asp Glu Ser

2785 2790 2795 2800

Gly Phe Leu Leu His Pro Ala Leu Leu Asp Ala Ala Leu His Pro Ile

2805 2810 2815

Gly Leu Gly Gly Leu Val Gly Asp Gly Gly Leu Pro Phe Ala Trp His

2820 2825 2830

Gly Leu Arg Val His Ala Val Gly Ala Arg Ala Ala Arg Val Arg Leu

2835 2840 2845

Thr Pro Leu Gly Asp Glu Thr Val Ala Val Asp Leu Ala Asp Gly Ala

2850 2855 2860

Gly Ala Pro Leu Ala Ala Ile Val Ser Leu Arg Leu Arg Ala Val Thr

2865 2870 2875 2880

Ala Ala Gln Val Ala Ala Ala Arg Thr Leu Thr Asp Pro Ala Leu His

2885 2890 2895

Leu Asp Trp Leu Pro Val Ala Gly Ala Ala Ser Ala Pro Ser Asp Asp

2900 2905 2910

Thr Ala Val Glu Leu Leu Arg Leu Asp Asp Ala Pro His Gly Thr Asp

2915 2920 2925

Ala Gly Pro Gly Gly Val Arg Gln Ala Val Arg Thr Ala Leu Thr Ala

2930 2935 2940

Ile Gln Glu Arg Ile Ala Asp Asp Arg Ala Glu Arg Leu Val Val Val

2945 2950 2955 2960

Thr Arg Asp Thr Asp Leu Ala Gly Ala Ala Val Gly Gly Leu Leu Arg

2965 2970 2975

Ser Ala Gln Ala Glu His Pro Gly Arg Phe Gly His Val Val Leu Asp

2980 2985 2990

Gly His Pro Asp Ser Glu Arg Ala Leu Pro Thr Ala Thr Ala Leu Thr

2995 3000 3005

Asp Glu Pro Trp Val Ala Val Arg Ala Gly Glu Thr Tyr Val Pro Arg

3010 3015 3020

Leu Ala Arg Ser Gly Ala Arg Pro Gly Gly Asp Val Pro Phe Gly Ala

3025 3030 3035 3040

Asp Asp Val Val Leu Val Thr Gly Gly Thr Gly Val Leu Gly Ala Leu

3045 3050 3055

Val Ala Arg His Leu Val Thr Glu His Gly Val Arg Arg Leu Val Leu

3060 3065 3070

Ala Ser Arg Arg Gly Gly Ala Gly Glu Leu Val Ala Glu Leu Gly Glu

3075 3080 3085

Leu Gly Ala Ala Val Asp Val Val Ala Cys Asp Val Ser Asp Arg Glu

3090 3095 3100

Ala Leu Glu Arg Leu Val Ala Ala His Pro Leu Thr Gly Val Val His

3105 3110 3115 3120

Ala Ala Gly Val Leu Asp Asp Gly Thr Val Glu Ser Leu Thr Pro Asp

3125 3130 3135

Arg Val Asp Gly Val Leu Arg Ala Lys Val Asp Gly Ala Trp His Leu

3140 3145 3150

His Glu Leu Thr Ala Ala Leu Asp Leu Arg Ala Phe Val Leu Phe Ser

3155 3160 3165

Ser Leu Ala Gly Val Val Gly Ser Ala Gly Gln Gly Gly Tyr Ala Ala

3170 3175 3180

Ala Asn Ala Ala Leu Asp Ala Leu Ala Glu Tyr Arg Arg Ala Glu Gly

3185 3190 3195 3200

Leu Pro Ala Thr Ser Ile Ala Trp Gly Leu Trp Ala Pro Ala Ser Ala

3205 3210 3215

Met Thr Ser Gly Ala Asp Thr Ala Arg Leu Ala Arg Ser Gly Ile Leu

3220 3225 3230

Pro Leu Pro Ala Asp Arg Ala Leu Glu Leu Phe Asp Thr Ala Cys Ala

3235 3240 3245

Ala Glu Thr Pro Leu Thr Val Ala Ala Arg Leu Asp Leu Thr Ala Phe

3250 3255 3260

Arg Ala Gln Gly Thr Arg Met Pro Val Val Leu Arg Ser Leu Ala Gly

3265 3270 3275 3280

Pro Ala Ala Arg Arg Thr Ala Arg Ala Gly Asp Ala Gly Thr Leu Arg

3285 3290 3295

Asp Arg Leu Ala Ala Gln Thr Pro Ala Glu Arg Thr Arg Thr Val Leu

3300 3305 3310

Asp Leu Val Arg Gly Gln Ala Ala Ala Val Leu Gly His Glu Ser Ala

3315 3320 3325

Ala Ala Ile Ala Glu Asp Arg Ala Phe Leu Glu Leu Gly Phe Asp Ser

3330 3335 3340

Leu Thr Ala Val Asp Leu Arg Asn Arg Leu Gly Thr Val Thr Gly Leu

3345 3350 3355 3360

Arg Leu Pro Thr Thr Thr Val Phe Asp His Pro Asn Pro Ala Ala Leu

3365 3370 3375

Thr Arg His Ile Leu Ala Glu Leu Leu Gly Ala Thr Ala Gly Ser Ala

3380 3385 3390

Gln Ala Ala Pro Thr Ala Val Arg Thr Asp Glu Pro Ile Ala Ile Val

3395 3400 3405

Gly Met Ala Cys Arg Tyr Pro Gly Gly Val Ala Ser Pro Glu Asp Leu

3410 3415 3420

Trp Arg Val Val Ala Glu Gly Arg Asp Val Ile Ser Pro Phe Pro Glu

3425 3430 3435 3440

Asp Arg Gly Trp Asp Leu Gly Ala Leu Tyr His Ala Asp Pro Asp His

3445 3450 3455

Thr Gly Thr Ser Tyr Ala Arg Glu Gly Gly Phe Leu His Asp Ala Ala

3460 3465 3470

Gly Phe Asp Ala Glu Phe Phe Gly Ile Ser Pro Arg Glu Ala Leu Ala

3475 3480 3485

Met Asp Pro Gln Gln Arg Leu Leu Leu Glu Ala Ser Trp Glu Ala Ile

3490 3495 3500

Glu Arg Ala Gly Ile Asp Pro Thr Ala Leu Arg Gly Ser Ala Thr Gly

3505 3510 3515 3520

Val Phe Ala Gly Leu Met Tyr His Asp Tyr Ala Ala Arg Leu Gly Thr

3525 3530 3535

Thr Pro Glu Gly Leu Glu Gly Tyr Leu Gly Met Gly Asn Ser Gly Ser

3540 3545 3550

Val Ala Ser Gly Arg Ile Ser Tyr Thr Leu Gly Leu Glu Gly Pro Ala

3555 3560 3565

Val Thr Val Asp Thr Ala Cys Ser Ser Ser Leu Val Ala Leu His Trp

3570 3575 3580

Ala Ile Gln Ala Leu Arg Ser Gly Glu Cys Asp Leu Ala Leu Ala Gly

3585 3590 3595 3600

Gly Val Ser Val Met Ala Thr Pro Gly Thr Phe Val Glu Phe Ser Arg

3605 3610 3615

Gln Arg Gly Leu Ala Pro Asp Gly Arg Cys Lys Ser Phe Ala Ala Ala

3620 3625 3630

Ala Asp Gly Ala Ser Trp Ser Glu Gly Val Gly Met Leu Leu Val Glu

3635 3640 3645

Arg Leu Ser Asp Ala Arg Arg Asn Gly His Arg Val Leu Ala Val Val

3650 3655 3660

Arg Gly Ser Ala Val Asn Gln Asp Gly Ala Ser Asn Gly Leu Thr Ala

3665 3670 3675 3680

Pro Asn Gly Pro Ser Gln Gln Arg Val Ile Arg Gln Ala Leu Ala Gly

3685 3690 3695

Ala Gly Leu Thr Ser Ala Asp Val Asp Ala Val Glu Ala His Gly Thr

3700 3705 3710

Gly Thr Arg Leu Gly Asp Pro Ile Glu Ala Gln Ala Leu Leu Ala Thr

3715 3720 3725

Tyr Gly Gln Glu Arg Asp Glu Pro Leu Trp Leu Gly Ser Val Lys Ser

3730 3735 3740

Asn Ile Gly His Ala Gln Ala Ala Ala Gly Val Ala Gly Val Ile Lys

3745 3750 3755 3760

Met Val Gln Ala Met Arg Asn Gly Val Leu Pro Arg Thr Leu His Val

3765 3770 3775

Asp Glu Pro Ser Pro His Val Asp Trp Thr Val Gly Ala Val Glu Leu

3780 3785 3790

Leu Thr Gly Glu Gln Glu Trp Pro Arg Gln Asp Arg Pro Arg Arg Ala

3795 3800 3805

Gly Val Ser Ser Phe Gly Val Ser Gly Thr Asn Ala His Val Val Leu

3810 3815 3820

Glu Glu Ala Pro Glu Glu Thr Glu Gly Thr Ala Pro Ala Ala Leu Pro

3825 3830 3835 3840

Ala Val Pro Trp Val Val Ser Ala Arg Ser Glu Thr Ala Leu Arg Ala

3845 3850 3855

Gln Ala Ala Arg Leu Ala Asp Trp Leu His Gly Asp Thr Asp Val Leu

3860 3865 3870

Gly Thr Ala Tyr Ser Leu Ala Thr Gly Arg Ala Ala Leu Pro His Arg

3875 3880 3885

Ala Val Val Val Gly Thr Asp Arg Ala Glu Leu Ser Asp Gly Leu Ala

3890 3895 3900

Ala Leu Ala Ala Gly Arg Ala Ala Ala His Val Glu Ser Gly Arg Ala

3905 3910 3915 3920

Arg Asp Asn Arg Val Thr Ala Phe Val Phe Ala Gly Gln Gly Ala Gln

3925 3930 3935

Arg Ala Gly Met Gly Ala Glu Leu Ala Ala Ala Tyr Pro Val Phe Ala

3940 3945 3950

Gln Val Phe Ala Gln Val Cys Ala Ala Phe Asp Gly Val Leu Glu Arg

3955 3960 3965

Pro Leu Gly Glu Val Ile Ala Glu Gly Gly Pro Glu Leu Asp Arg Thr

3970 3975 3980

Val Tyr Ala Gln Ala Gly Leu Phe Ala Phe Glu Val Ala Leu Phe Arg

3985 3990 3995 4000

Leu Leu Glu Ser Trp Gly Val Ala Pro Asp Val Val Leu Gly His Ser

4005 4010 4015

Val Gly Glu Leu Ala Ala Ala Cys Val Ala Gly Val Trp Ser Leu Glu

4020 4025 4030

Asp Ala Val Arg Val Val Ala Ala Arg Gly Arg Leu Met Gln Ala Leu

4035 4040 4045

Pro Gln Gly Gly Ala Met Val Ala Leu Glu Val Ser Ala Gly Glu Leu

4050 4055 4060

Glu Leu Pro Glu Gly Val Glu Leu Ala Ala Val Asn Gly Pro Ser Ser

4065 4070 4075 4080

Val Val Leu Ser Gly Glu Glu Glu Ala Val Leu Ala Glu Ala Ala Arg

4085 4090 4095

Trp Pro Asp Arg Arg Ala Lys Arg Leu Arg Val Ser His Ala Phe His

4100 4105 4110

Ser Arg Arg Met Asp Pro Met Leu Glu Asp Phe Arg Arg Val Leu Glu

4115 4120 4125

Ser Val Ala Phe His Ala Pro Glu Leu Val Phe Val Ser Thr Val Thr

4130 4135 4140

Gly Ala Val Val Thr Asp Glu Leu Cys Asp Pro Gly Tyr Trp Val Arg

4145 4150 4155 4160

Asn Val Arg Glu Thr Val Arg Phe Ala Asp Ala Val Val Ala Ala Glu

4165 4170 4175

Ala Gly Val Phe Val Glu Leu Ala Pro Asp Ala Val Leu Ser Gly Leu

4180 4185 4190

Val Gly Glu Ser Val Glu Gly Val Leu Cys Val Pro Ala Gln Arg Ala

4195 4200 4205

Gly Arg Pro Ala Ala Arg Val Leu Val Ser Ala Leu Gly Thr Leu His

4210 4215 4220

Thr His Gly Val Asp Val Ala Trp Gly Arg Phe Phe Ala Gly Ser Gly

4225 4230 4235 4240

Ala Arg Arg Val Asp Leu Pro Thr Tyr Ala Phe Gln Arg Glu Arg Tyr

4245 4250 4255

Trp Leu Asp Ala Pro Ala Pro Gln Thr Gly Gly Ala Ser Asp Asp Ala

4260 4265 4270

Phe Trp Ala Ala Val Gln Ser Gly Asp Leu Ala Gly Leu Leu Gly Val

4275 4280 4285

Ala Glu Asp Ala Gly Leu Asp Ala Val Leu Pro Thr Leu Ala Ser Trp

4290 4295 4300

His Asp Arg Glu Arg Ala Asp Ala Val Val Asp Gly Trp Arg His Lys

4305 4310 4315 4320

Val Arg Trp Thr Pro Leu Pro Glu Ala Gly Gly Ala Val Leu Ser Gly

4325 4330 4335

Arg Trp Leu Leu Val Gly Pro Glu Gly Asp Glu Ala Leu Val Ala Asp

4340 4345 4350

Val Ala Thr Ala Leu Arg Glu His Gly Ala Glu Val Thr His Leu Ala

4355 4360 4365

Leu Pro Arg Asp Ala Asp Arg Glu Thr Thr Ala Glu Leu Leu Arg Gly

4370 4375 4380

Thr Asp Asp Thr Gly Leu Thr Ala Val Leu Ser Leu Leu Ala Arg Ala

4385 4390 4395 4400

Asp Arg Pro Val His Ala Thr Leu Ala Leu Val Gln Ala Leu Gly Asp

4405 4410 4415

Ala Glu Val Thr Val Pro Leu Trp Cys Ala Thr Ser Glu Ser Val Ala

4420 4425 4430

Val Ala Ser Thr Asp Val Val Pro Glu Ala Ala Val Asp Ala Ala Gly

4435 4440 4445

Leu Trp Gly Leu Gly Arg Val Val Arg Leu Glu Ala Pro Asp Arg Trp

4450 4455 4460

Gly Gly Leu Val Asp Leu Pro Gly Ala Leu Asp Ala Arg Ala Arg Arg

4465 4470 4475 4480

Arg Leu Ala Ala Val Leu Ala Gly Ala Glu Asp Glu Cys Ala Val Arg

4485 4490 4495

Glu Asn Gly Ala Phe Ala Ala Arg Leu Val Arg Ala Ala Asp Ala Pro

4500 4505 4510

Arg Arg Glu Trp Arg Pro Gln Gly Thr Val Leu Val Thr Gly Gly Thr

4515 4520 4525

Gly Ala Leu Gly Ala Arg Val Ala Gln Arg Leu Ala Glu Arg Gly Ala

4530 4535 4540

Arg His Val Leu Leu Val Ser Arg Arg Gly Pro Ala Ala Asp Gly Ala

4545 4550 4555 4560

Ala Glu Leu Val Arg Ala Ile Glu Ala Ala Gly Ala Thr Ala Thr Val

4565 4570 4575

Ala Ala Cys Asp Val Ala Asp Pro Glu Ala Val Ala Ala Leu Leu Glu

4580 4585 4590

Arg Ile Pro Ala Asp Ala Pro Leu Thr Ala Val Val His Thr Ala Ser

4595 4600 4605

Val Leu Ala Asp Ala Pro Leu Asp Thr Leu Thr Pro Asp Arg Ile Thr

4610 4615 4620

Ala Val Leu Arg Ala Lys Ala Asp Ala Ala Arg Ile Leu His Thr Ala

4625 4630 4635 4640

Thr Ala Ser Leu Asp Leu Asp Ala Phe Val Leu Phe Ser Ser Leu Ala

4645 4650 4655

Gly Thr Leu Gly Asn Pro Gly Gln Ala Ala Tyr Ala Ala Ala Asn Ala

4660 4665 4670

Val Leu Asp Thr Leu Ala Ala His Arg Arg Ala Leu Gly Leu Pro Gly

4675 4680 4685

Thr Ala Val Ala Trp Gly Pro Trp Ala Gly Gly Gly Met Leu Asp Asp

4690 4695 4700

Thr Val Ala Glu Arg Leu Arg Arg Ala Gly Val Ile Pro Leu Asp Pro

4705 4710 4715 4720

Glu His Ala Leu Val Ala Leu Asp Arg Ala Val Ala Ala Ala Asp Ala

4725 4730 4735

His Ser Val Ile Ala Asp Ala Asp Trp Thr Gly Leu Thr Ala Gly Ser

4740 4745 4750

Met Leu Ala Glu Leu Ser Gly Pro Ala Pro Val Glu Ala Pro Thr Ala

4755 4760 4765

Leu Thr Gly Pro Asp Arg Glu Arg Ala Ala Leu Ala Leu Val Arg Ser

4770 4775 4780

Cys Ala Ala Ala Val Leu Gly Arg Ser Ala Ala Val Asp Val Glu Pro

4785 4790 4795 4800

Asp Thr Ala Phe Arg Glu Leu Gly Phe Asp Ser Met Ala Ala Val Gln

4805 4810 4815

Leu Arg Asn Arg Leu Asn Ala Ala Thr Gly Val Val Leu Thr Ala Thr

4820 4825 4830

Ala Val Phe Asp His Pro Ser Ala Arg Ala Leu Ala Thr His Leu Leu

4835 4840 4845

Ala Leu Ala Thr Gly Glu Gly Ala Gly Ser Ala Gly Ala Thr Glu Leu

4850 4855 4860

Pro Gly Thr Ala Val His Thr Asp Glu Pro Ile Ala Ile Val Gly Met

4865 4870 4875 4880

Ala Cys Arg Phe Pro Gly Asp Val Thr Ser Pro Glu Asp Leu Trp Arg

4885 4890 4895

Leu Leu Ala Asp Gly Val Asp Ala Val Gly Pro Ile Pro Ala Asp Arg

4900 4905 4910

Gln Trp Gly Pro Thr Asp Ala Tyr Ala Glu Gly Gly Phe Leu Arg Gly

4915 4920 4925

Ala Gly Glu Phe Asp Ala Asp Phe Phe Gly Ile Ser Pro Arg Glu Ala

4930 4935 4940

Leu Val Met Asp Pro Gln Gln Arg Leu Ala Leu Glu Thr Gly Trp Glu

4945 4950 4955 4960

Val Phe Glu Arg Ala Gly Ile Asn Pro His Thr Val Arg Gly Thr Ser

4965 4970 4975

Val Gly Val Phe Leu Gly Thr Asn Gly Gln Asp Tyr Val Ser Leu Leu

4980 4985 4990

Ala Gly Ala Thr Glu Ala His Ala Gly His Ile Gly Thr Gly Asn Ser

4995 5000 5005

Ala Ser Val Leu Ser Gly Arg Ile Ala Tyr Val Leu Gly Leu Glu Gly

5010 5015 5020

Pro Ala Val Thr Val Asp Thr Ala Cys Ser Ser Ser Leu Val Ala Leu

5025 5030 5035 5040

His Trp Ala Ile Gln Ala Leu Arg Ser Gly Glu Cys Ser Met Ala Leu

5045 5050 5055

Ala Gly Gly Val Thr Val Met Ala Thr Pro Gly Ala Phe Ala Glu Phe

5060 5065 5070

Ser His Gln Arg Gly Leu Ala Glu Asp Gly Arg Cys Lys Ser Phe Ala

5075 5080 5085

Ala Ser Ala Asp Gly Thr Gly Trp Gly Glu Gly Val Gly Met Leu Leu

5090 5095 5100

Val Glu Arg Leu Ser Asp Ala Arg Arg Asn Gly His Arg Val Leu Ala

5105 5110 5115 5120

Val Val Arg Gly Ser Ala Val Asn Gln Asp Gly Ala Ser Asn Gly Leu

5125 5130 5135

Thr Ala Pro Asn Gly Pro Ser Gln Gln Arg Val Ile Arg Gln Ala Leu

5140 5145 5150

Ala Gly Ala Gly Leu Thr Pro Val Asp Val Asp Ala Val Glu Ala His

5155 5160 5165

Gly Thr Gly Thr Arg Leu Gly Asp Pro Ile Glu Ala Gln Ala Leu Leu

5170 5175 5180

Ala Thr Tyr Gly Gln Glu Arg Asp Glu Pro Leu Trp Leu Gly Ser Val

5185 5190 5195 5200

Lys Ser Asn Ile Gly His Thr Gln Ala Ala Ala Gly Val Ala Gly Val

5205 5210 5215

Ile Lys Met Val Glu Ala Met Arg Asn Gly Thr Leu Pro Pro Thr Leu

5220 5225 5230

His Val Asp Glu Pro Ser Pro His Val Asp Trp Ser Ala Gly Ala Val

5235 5240 5245

Glu Leu Leu Thr Glu Ala Arg Glu Trp Lys Arg Ala Gly Arg Pro Arg

5250 5255 5260

Arg Ala Gly Val Ser Ser Phe Gly Val Ser Gly Thr Asn Ala His Val

5265 5270 5275 5280

Val Leu Glu Glu Ala Pro Glu Glu Thr Glu Pro Ala Ala Ser Asp Glu

5285 5290 5295

Leu Pro Val Ala Pro Trp Leu Leu Ser Ala Arg Ser Glu Lys Ala Leu

5300 5305 5310

Leu Ala Gln Val Glu Arg Leu Arg Ala Tyr Val Thr Glu His Pro Glu

5315 5320 5325

Ala Arg Pro Ala Asp Ile Gly Leu Ser Leu Ala Thr Gly Arg Ala Ala

5330 5335 5340

Leu Ala His Arg Leu Ser Gly Ala Gly Glu Thr Thr Glu Glu Leu Leu

5345 5350 5355 5360

Ala Ala Leu Asp Thr Ala Leu Pro Ala Val Ala Arg Glu Thr Pro Thr

5365 5370 5375

Ala Phe Val Phe Ala Gly Gln Gly Ala Gln Arg Val Gly Met Gly Ala

5380 5385 5390

Glu Leu Ala Ala Val Tyr Pro Val Phe Ala Gln Val Phe Ala Gln Val

5395 5400 5405

Cys Ala Ala Phe Asp Gly Val Leu Glu Arg Pro Leu Gly Glu Val Val

5410 5415 5420

Ala Glu Gly Gly Pro Glu Leu Asp Arg Thr Val Tyr Ala Gln Ala Gly

5425 5430 5435 5440

Leu Phe Ala Phe Glu Val Ala Leu Phe Arg Leu Leu Glu Ser Trp Gly

5445 5450 5455

Val Ala Pro Asp Val Val Leu Gly His Ser Val Gly Glu Leu Ala Ala

5460 5465 5470

Ala Cys Val Ala Gly Val Trp Ser Leu Glu Asp Ala Val Arg Val Val

5475 5480 5485

Ala Ala Arg Gly Arg Leu Met Gln Ala Leu Pro Gln Gly Gly Ala Met

5490 5495 5500

Val Ala Leu Glu Val Ser Ala Gly Glu Leu Glu Leu Pro Glu Gly Val

5505 5510 5515 5520

Glu Leu Ala Ala Val Asn Gly Pro Thr Ser Val Val Leu Ser Gly Glu

5525 5530 5535

Glu Asp Ala Val Leu Ala Glu Ala Ala Arg Trp Pro Asp Arg Arg Ala

5540 5545 5550

Lys Arg Leu Arg Val Ser His Ala Phe His Ser Arg Arg Met Asp Pro

5555 5560 5565

Met Leu Glu Asp Phe Arg Arg Val Leu Glu Ser Val Thr Phe His Ala

5570 5575 5580

Pro Gln Leu Ala Phe Val Ser Thr Val Thr Gly Ala Ala Val Thr Asp

5585 5590 5595 5600

Glu Leu Cys Asp Pro Gly Tyr Trp Val Arg Asn Val Arg Glu Thr Val

5605 5610 5615

Arg Phe Ala Asp Ala Val Val Ala Ala Gly Ala Gly Val Phe Val Glu

5620 5625 5630

Leu Ala Pro Asp Ala Val Leu Ser Gly Leu Val Gly Glu Ser Val Glu

5635 5640 5645

Gly Val Leu Cys Val Pro Ala Gln Arg Ala Gly Arg Pro Ala Ala Arg

5650 5655 5660

Ala Leu Val Ser Ala Leu Gly Thr Leu His Thr His Gly Val Asp Val

5665 5670 5675 5680

Ala Trp Asp Arg Phe Phe Ala Gly Ser Gly Ala Arg Arg Val Asp Leu

5685 5690 5695

Pro Thr Tyr Ala Phe Gln Arg Glu Arg Tyr Trp Leu Ala Pro Pro Ala

5700 5705 5710

Ala Gly Pro Val Gly Leu Ala Gly Val Gly Leu Thr Ala Thr Gly His

5715 5720 5725

Pro Leu Leu Gly Val Ser Val Glu Leu Pro Gly Thr Asp Ala Val Ala

5730 5735 5740

Phe Thr Gly Ser Val Ser Leu Ser Thr His Pro Trp Leu Ala Asp His

5745 5750 5755 5760

Ala Ile Leu Gly Pro Ala Leu Leu Pro Gly Thr Ala Phe Leu Asp Leu

5765 5770 5775

Ala Leu Ala Ala Gly Glu His Val Gly Cys Pro Arg Val Asp Asp Leu

5780 5785 5790

Ala Leu His Ala Pro Leu Ala Leu Pro Ala His Gly Ser Val Arg Met

5795 5800 5805

Gln Val Arg Val Glu Gly Thr Asp Pro Asp Gly Ser Arg Gln Val Gly

5810 5815 5820

Ile Tyr Ser Arg Pro Glu Asp Asp Glu Asp Ala Trp Thr Gln His Ala

5825 5830 5835 5840

Thr Gly Val Leu Ala Pro Glu Ser Gly Pro Ala Ala Glu Ala Leu Thr

5845 5850 5855

Glu Trp Pro Pro Gln Gly Ala Glu Pro Val Ser Val Asp Gly Leu Tyr

5860 5865 5870

Asp Asp Leu Ala Ala Thr Gly Phe Ala Tyr Gly Pro Leu Phe Arg Gly

5875 5880 5885

Leu Arg Ala Ala Trp Val Arg Asp Gly Ala Val Tyr Ala Asp Val Ala

5890 5895 5900

Leu Pro Asp Glu Thr Ala Ser Val Ala Gly Tyr Ser Val His Pro Ala

5905 5910 5915 5920

Leu Leu Asp Ala Ala Leu His Ala Leu Gly Cys Ala Asn Leu Val Ala

5925 5930 5935

Asp Trp Ala Asp Gly Gln Leu Pro Phe Ala Trp Thr Gly Ala Arg Ile

5940 5945 5950

His Ala Val Gly Ala Arg Ala Leu Arg Val Arg Leu Arg Ala Glu Asn

5955 5960 5965

Gly Gly Ile Ala Leu Thr Ala Ala Asp Ala Gly Gly Gln Pro Val Ala

5970 5975 5980

Gly Ile Glu Gly Val Arg Leu Arg Pro Val Gly Asp Ala Arg Ala Val

5985 5990 5995 6000

Arg Ala Ala Ala Gly Ala Arg Pro Cys Arg Val Glu Trp Leu Pro Val

6005 6010 6015

Val Pro Ala Asp Asp Pro Glu Asp Asp Asp Ile Thr Val Val Pro Val

6020 6025 6030

Thr Gly Arg Thr Ala Gly Gly Asp Val Val Ala Glu Val Arg Ala Ala

6035 6040 6045

Val Ala Ala Ala Leu Ala Arg Leu Gln Glu Trp Leu Ala Asp Asp Arg

6050 6055 6060

Ser Glu Arg Leu Val Leu Val Thr Arg Gly Ala Val Ala Ala Leu Pro

6065 6070 6075 6080

Asp Glu Ala Pro Asp Pro Val Ala Ala Ala Val Trp Gly Leu Val Arg

6085 6090 6095

Ser Ala Gln Ser Glu His Pro Gly Arg Phe Val Leu Ala Asp Leu Ala

6100 6105 6110

Ala Ser Glu Asp Ala Ala Val Val Ser Ala Ala Val Ala Thr Gly Glu

6115 6120 6125

Pro Gln Ile Ala Val Arg Asn Gly Ala Leu Tyr Ala Pro Arg Leu Val

6130 6135 6140

Arg Gly Ala Ser Arg Pro Gly Gly Glu Val Pro Phe Gly Ala Gly Asp

6145 6150 6155 6160

Val Val Leu Val Thr Gly Gly Thr Gly Ala Leu Gly Arg Val Val Ala

6165 6170 6175

Arg His Leu Val Ala Glu His Gly Val Arg Arg Leu Val Leu Ala Ser

6180 6185 6190

Arg Arg Gly Gly Ala Gly Glu Leu Val Ala Glu Leu Gly Glu Leu Gly

6195 6200 6205

Ala Ala Val Asp Val Val Ala Cys Asp Val Ser Asp Arg Glu Ala Leu

6210 6215 6220

Glu Gly Leu Val Ala Ala Tyr Pro Leu Thr Gly Val Val His Ala Ala

6225 6230 6235 6240

Gly Val Leu Asp Asp Gly Thr Val Glu Ser Leu Thr Ala Glu Arg Val

6245 6250 6255

Asp Gly Val Leu Arg Ala Lys Val Asp Gly Ala Trp His Leu His Glu

6260 6265 6270

Leu Thr Ser Gly Leu Asp Leu Arg Ala Phe Val Leu Phe Ser Ser Leu

6275 6280 6285

Ala Gly Val Val Gly Ser Ala Gly Gln Gly Gly Tyr Ala Ala Ala Asn

6290 6295 6300

Ala Ala Leu Asp Ala Leu Ala Glu Tyr Arg Arg Ala Glu Gly Leu Pro

6305 6310 6315 6320

Ala Thr Ser Ile Ala Trp Gly Pro Trp Thr Asp Gly Met Thr Thr Gly

6325 6330 6335

Leu Glu Arg Ala Asp Arg Ala Arg Ile Ser Arg Ser Gly Thr Arg Ala

6340 6345 6350

Leu Gly Thr Glu Asp Gly Leu Ala Leu Phe Asp Gln Ala Val Arg Gly

6355 6360 6365

Ala Asp Ala Leu Ala Val Ala Ala Leu Trp Asp Leu Ser Ala Leu Arg

6370 6375 6380

Ala Ala Gln Ser Leu Pro Pro Leu Phe Gln Gly Leu Ala Gly Arg Pro

6385 6390 6395 6400

Val Arg Arg Thr Val Ala Asp Gly Ala Gly Arg Gly Ala Glu Trp Ala

6405 6410 6415

Asp Arg Phe Thr Gly Leu Ser Pro Ala Glu Ser Glu Arg Ala Ala Val

6420 6425 6430

Glu Trp Val Arg Glu Gln Ala Ala Ala Val Leu Gly His Ala Ser Ser

6435 6440 6445

Ala Ala Val Ala Ala Asp Arg Ala Phe Leu Asp Leu Gly Phe Asp Ser

6450 6455 6460

Leu Thr Ala Val Glu Leu Arg Asn Arg Leu Ala Thr Ala Thr Gly Leu

6465 6470 6475 6480

Arg Leu Gly Thr Thr Ala Val Phe Asp Tyr Pro Thr Pro Ala Gly Leu

6485 6490 6495

Ala Ala His Leu Leu Glu Arg Val Leu Gly Ala Thr Thr Gly Ser Ala

6500 6505 6510

Gln Pro Ala Pro Ala Ala Val Pro Ala Asp Glu Pro Ile Ala Ile Val

6515 6520 6525

Gly Met Ala Cys Arg Tyr Pro Gly Gly Ile Thr Ser Pro Glu Glu Leu

6530 6535 6540

Trp Arg Glu Val Ala Glu Gly Arg Asp Ala Ile Ser Gly Phe Pro Thr

6545 6550 6555 6560

Asp Arg Gly Trp Asp Leu Arg Ala Leu Phe Ala Asp Asp Pro Gly Arg

6565 6570 6575

Pro Gly Thr Ser His Thr Arg Glu Gly Gly Phe Leu His Asp Ala Gly

6580 6585 6590

Glu Phe Asp Ala Asp Phe Phe Gly Ile Ser Pro Arg Glu Ala Leu Ala

6595 6600 6605

Met Asp Pro Gln Gln Arg Leu Leu Leu Glu Ala Ser Trp Glu Ala Ile

6610 6615 6620

Glu Arg Ala Gly Ile Asp Pro Ala Ser Leu Arg Gly Ser Arg Thr Gly

6625 6630 6635 6640

Val Tyr Ala Gly Val Met Tyr His Asp Tyr Ala Ala Arg Val Asp Val

6645 6650 6655

Leu Pro Asp Gly Val Glu Gly Tyr Leu Gly Thr Gly Asn Ser Gly Ser

6660 6665 6670

Ile Ala Ser Gly Arg Ile Ala Tyr Ala Leu Gly Leu Glu Gly Gln Ala

6675 6680 6685

Val Thr Val Asp Thr Ala Cys Ser Ser Ser Leu Val Ala Leu His Trp

6690 6695 6700

Ala Val Arg Ser Leu Arg Ser Gly Glu Ser Asp Leu Ala Leu Ala Gly

6705 6710 6715 6720

Gly Val Thr Val Met Ala Thr Pro Gly Val Phe Val Asp Phe Ser Arg

6725 6730 6735

Gln Arg Gly Leu Ala Thr Asp Gly Arg Cys Lys Ser Tyr Gly Ala Gly

6740 6745 6750

Ala Asp Gly Thr Gly Trp Ser Glu Gly Val Gly Met Leu Leu Val Glu

6755 6760 6765

Arg Leu Ser Asp Ala Arg Arg Asn Gly His Arg Val Leu Ala Val Ile

6770 6775 6780

Arg Gly Thr Ala Val Asn Gln Asp Gly Ala Ser Asn Gly Leu Thr Ala

6785 6790 6795 6800

Pro Asn Gly Pro Ser Gln Gln Arg Val Ile Arg Gln Ala Leu Ala Asp

6805 6810 6815

Ala Ser Leu Arg Pro Ser Asp Val Asp Ala Val Glu Gly His Gly Thr

6820 6825 6830

Gly Thr Ser Leu Gly Asp Pro Ile Glu Val Glu Ala Leu Leu Ala Thr

6835 6840 6845

Tyr Gly Gln Glu Arg Asp Glu Pro Leu Trp Leu Gly Ser Ile Lys Ser

6850 6855 6860

Asn Ile Gly His Thr Gln Ala Ala Ala Gly Val Ala Gly Val Ile Lys

6865 6870 6875 6880

Met Val Glu Ala Met Arg His Gly Val Leu Pro Arg Thr Leu His Ala

6885 6890 6895

Asp Glu Pro Ser Pro His Ile Asp Trp Ala Ser Gly Ala Val Glu Leu

6900 6905 6910

Leu Ala Glu Gln Arg Gln Trp Pro Arg Thr Asp Arg Pro Arg Arg Ala

6915 6920 6925

Ala Val Ser Ser Phe Gly Leu Ser Gly Thr Asn Ala His Val Val Leu

6930 6935 6940

Glu His Thr Asp His Gly Asp His His Ser Ala Gly Thr Gly Glu Arg

6945 6950 6955 6960

Pro Ala Val Pro Val Pro Val Pro Val Pro Val Ala Leu Ser Ala Arg

6965 6970 6975

Thr Asp Ala Gly Leu Arg Ala Gln Ala Asp Arg Leu Ala Ala Ala Leu

6980 6985 6990

Thr Ala Asp Pro Asp Leu Ile Pro Leu Asp Ile Ala Tyr Ser Ala Val

6995 7000 7005

Thr Gly Arg Ala Arg Leu Glu Arg Arg Ala Ser Val Val Ala Ala Thr

7010 7015 7020

Arg Glu Glu Leu Leu Ala Gly Leu Gly Asp Leu Gly Pro Ala Thr Val

7025 7030 7035 7040

Ala Gly Ala Gly His Thr Ala Phe Leu Phe Thr Gly Gln Gly Ala Gln

7045 7050 7055

Arg Pro Gly Thr Gly Glu Glu Leu Ala Ala Ala His Pro Val Phe Ala

7060 7065 7070

Ala Ala Tyr Ala Glu Val Cys Thr Ala Phe Asp Ala Val Leu Asp Arg

7075 7080 7085

Pro Leu Arg Glu Val Val Ala Thr Gly Asp Gly Leu Asp Asp Thr Gly

7090 7095 7100

Tyr Gly Gln Pro Ala Val Phe Ala Leu Glu Val Ala Phe Gly Arg Leu

7105 7110 7115 7120

Phe Glu Ser Trp Gly Val Val Pro Asp Phe Leu Leu Gly His Ser Val

7125 7130 7135

Gly Glu Leu Ala Ala Ala His Leu Ala Gly Val Trp Ser Leu Pro Asp

7140 7145 7150

Ala Val Arg Val Val Ala Ala Arg Ser Arg Leu Met Ala Ala Leu Pro

7155 7160 7165

Ala Gly Gly Ala Met Ala Ala Val Glu Ala Ser Ala Asp Glu Val Ala

7170 7175 7180

Ala Glu Leu Ala Asp Gly Ala Val Leu Ala Ala Val Asn Gly Pro Arg

7185 7190 7195 7200

Ser Val Val Val Ser Gly Ala Arg Asp Ala Val Leu Ala Thr Ala Gly

7205 7210 7215

Leu Trp Ala Ala Arg Gly Cys Arg Thr Arg Glu Leu Lys Val Ser His

7220 7225 7230

Ala Phe His Ser Pro Leu Met Glu Pro Met Leu Ser Asp Phe Ala Ala

7235 7240 7245

Ala Leu Ala Asp Val Glu Phe Arg Ala Pro Arg Ile Pro Leu Val Ser

7250 7255 7260

Thr Val Thr Gly Ala Val Ala Gly Asp Glu Leu Cys Thr Pro Gly Tyr

7265 7270 7275 7280

Trp Val Arg His Val Arg Asp Thr Val Arg Phe Ala Asp Gly Val Arg

7285 7290 7295

Ala Leu Gly Gln Ala Gly Val Asp Thr Val Val Glu Leu Gly Pro Asp

7300 7305 7310

Gly Val Leu Thr Ala Met Ala Ala Pro Leu Leu Pro Asp Thr Ala Val

7315 7320 7325

Ala Leu Pro Thr Leu Arg Ala Gly Arg Pro Glu Ala Pro Ala Val Ala

7330 7335 7340

Ala Ala Leu Gly Ala Leu His Asp Arg Gly Thr Ala Val Asp Trp Pro

7345 7350 7355 7360

Ala Phe Phe Arg Gly Thr Gly Ala Arg Thr Val Glu Leu Pro Thr Thr

7365 7370 7375

Ala Phe Gln Arg Thr Arg Tyr Trp Leu Glu Ser Ala Ala Arg Thr Gly

7380 7385 7390

Asp Leu Ser Ala Ala Gly Leu Ala Ala Ala Gly His Pro Leu Leu Gly

7395 7400 7405

Ala Ala Val Asp Ser Pro Asp Gly Met Leu Leu Thr Gly Arg Leu Asp

7410 7415 7420

Thr Ala Thr His Pro Trp Leu Ala Asp His Thr Val Leu Asp Thr Val

7425 7430 7435 7440

Leu Leu Pro Gly Thr Ala Phe Val Glu Leu Ala Arg Ala Ala Gly Glu

7445 7450 7455

Arg Val Gly Leu Pro Arg Val Arg Glu Leu Thr Leu Ala Ala Pro Leu

7460 7465 7470

Val Leu Pro Ala Asp Gly Ser Val Leu Val Gln Val His Val Gly Ala

7475 7480 7485

Ala Val Asp Gly Glu Arg Pro Val Thr Val Ser Ala Arg Thr Asp Asp

7490 7495 7500

Gly Gln Asp Trp Ala Arg His Ala Thr Gly Val Leu Ala Pro Ala Ala

7505 7510 7515 7520

Pro Ala Pro Gly Ala Asp Pro Leu Pro Trp Pro Pro Arg Asp Ala Glu

7525 7530 7535

Pro Val Ala Thr Ala Gly His Tyr Asp Glu Leu Ala Ala Ala Gly Leu

7540 7545 7550

Gly Tyr Gly Pro Ala Phe Arg Ala Leu Arg Ala Val Trp Arg Arg Gly

7555 7560 7565

Asp Gly Pro Ala Ala Glu Val Phe Ala Glu Ile Gly Pro Ala Pro Gly

7570 7575 7580

Thr Asp Pro Ala Gly Phe Gly Val His Pro Ala Leu Leu Asp Thr Ala

7585 7590 7595 7600

Leu His Ala Ala Ala Val Gly Gly Leu Gly Val Ala Gly Val Pro Phe

7605 7610 7615

Thr Trp Asn Glu Val Ala Val His Thr Ala Gly Ala Arg Ser Leu Arg

7620 7625 7630

Val Arg Ile Ala Pro Asp Gly Ser Gly Gly Leu Ser Leu Arg Ala Thr

7635 7640 7645

Asp Asp Glu Gly Arg Ala Val Ile Asp Val Gly Ala Leu Arg Leu Arg

7650 7655 7660

Ala Ile Ala Pro Glu Asp Leu Arg Thr Ala Pro Ala Ala Ala Glu Ala

7665 7670 7675 7680

Leu Phe Asp Leu Asp Trp Ala Pro Val Ser Val Ala Arg Gly Ala Arg

7685 7690 7695

Pro Ala Gly Arg Trp Ala Val Leu Ala Pro Ala Asp Ala Glu Leu Thr

7700 7705 7710

Asp Val Leu Gly Ala Gly Ile Glu Thr Val Lys Asp Leu Ala Thr Val

7715 7720 7725

Pro Asp Asp Leu Asp Ile Val Leu Ala Ala Val Ser Asp Arg Gly Gly

7730 7735 7740

Ala Gly Val Asp Ala Pro Thr Ala Ala Ser Val Gly Ala Ala Ala Glu

7745 7750 7755 7760

Thr Asp Val Pro Glu Ala Val Arg Ala Ile Leu Arg Gly Ala Leu Gly

7765 7770 7775

Leu Val Gln Thr Trp Val Ala Gly Glu Ser Ala Ala Arg Leu Val Leu

7780 7785 7790

Leu Thr Arg Gly Ala Val Ala Leu Ala Gly Glu Arg Pro Asp Leu Ala

7795 7800 7805

Gly Ala Ala Ala Trp Gly Leu Val Arg Ala Ala Gln Ser Glu His Pro

7810 7815 7820

Gly Arg Leu Val Leu Ala Asp Val Asp Asp Asp Pro Ala Ser Leu Ala

7825 7830 7835 7840

Ala Leu Pro Ala Ala Leu Ala Thr Gly Glu Pro Gln Leu Leu Ile Arg

7845 7850 7855

Ala Gly Ala Val Arg Ala Ala Arg Leu Val Arg Ala Ala Pro Ala Glu

7860 7865 7870

Pro Ala Val Pro Ala Ala Pro Leu Gly Ala Arg Gly Thr Val Leu Val

7875 7880 7885

Thr Gly Ala Thr Gly Ser Leu Gly Thr Leu Val Val Arg His Leu Val

7890 7895 7900

Ala Glu His Gly Val Arg Arg Leu Leu Leu Val Ser Arg Gln Gly Arg

7905 7910 7915 7920

Gln Pro Glu Pro Ala Ala Glu Leu Ala Ala Ala Gly Ala Glu Val Arg

7925 7930 7935

Phe Ala Ala Cys Asp Val Ala Asp Arg Asp Ala Leu Ala Ala Leu Leu

7940 7945 7950

Ala Ser Val Asp Pro Glu His Pro Val Thr Ala Val Val His Ala Ala

7955 7960 7965

Gly Val Leu Asp Asp Gly Val Val Ala Ala Leu Thr Pro Asp Arg Leu

7970 7975 7980

Asp Thr Val Leu Arg Pro Lys Ala Asp Ala Ala Trp His Leu His Glu

7985 7990 7995 8000

Leu Thr Gly Glu Leu Asp Ala Phe Val Leu Phe Ser Ser Ala Ala Gly

8005 8010 8015

Leu Leu Gly Ala Pro Gly Gln Ala Asn Tyr Ala Ala Ala Asn Ala Phe

8020 8025 8030

Leu Asp Ala Leu Ala Ala His Arg Arg Ala Ala Gly Leu Pro Ala Val

8035 8040 8045

Ser Ile Ala Trp Gly Pro Trp Ala Asp Gly Met Ala Ala Gln Leu Asp

8050 8055 8060

Thr Arg Arg Ala Ser Arg Ala Gly Leu Leu Pro Leu Asp Ala Ala Leu

8065 8070 8075 8080

Gly Leu Ala Leu Phe Asp Thr Ala Arg Thr Gly Ala Pro Thr Ala Pro

8085 8090 8095

Leu Ala Ala Arg Leu Asp Leu Pro Gly Leu Arg Ala Ala Ala Ala Glu

8100 8105 8110

Leu Pro Pro Ile Leu Arg Thr Leu Val Pro Ala Pro Ala Arg Thr Ala

8115 8120 8125

Pro Glu Pro Ala Glu Pro Leu Ala Asp Thr Leu Ala Ala Leu Pro Ala

8130 8135 8140

Glu Glu Arg Glu Arg Arg Ala Leu Asp Ala Val Leu Arg His Thr Ala

8145 8150 8155 8160

Glu Val Leu Gly His Ala Thr Ala Asp Gly Val Asp Arg Glu Arg Gly

8165 8170 8175

Phe Gln Gln Leu Gly Phe Asp Ser Leu Met Ser Val Glu Leu Arg Asn

8180 8185 8190

Arg Leu Gly Ala Ala Ala Gly Leu Arg Leu Pro Ala Thr Val Ile Phe

8195 8200 8205

Asp His Pro Thr Pro Ala Ala Leu Ala Ala His Leu Val Ala Glu Leu

8210 8215 8220

Ala Pro Gly Arg Pro Thr Val Ala Ser Leu Arg Thr Ser Thr Leu Ala

8225 8230 8235 8240

Glu Leu Glu Ala Ala Ala Asn Glu Phe Ala Gly Asp Pro Glu Leu Arg

8245 8250 8255

Glu Gly Leu Arg Thr Arg Leu Arg Ala Leu Leu Arg Thr Leu Asp Asp

8260 8265 8270

Pro Ala Pro Asp Glu Pro Leu Glu Glu Thr Gly Glu Glu Ser Leu Ala

8275 8280 8285

Glu Leu Leu Asp Leu Ala Asp Arg Glu Leu Gly Asp Phe

8290 8295 8300

<210>12

<211>3406

<212>PRT

<213>Streptomyces flaveolus

<400>1

Met Thr Glu His Asp Arg Thr Thr Asp Arg Ala Thr Ala Arg Gly Ala

1 5 10 15

Thr Ala Leu Ser Gln Glu Gln Thr Asp Asp Arg Lys Val Val Glu Thr

20 25 30

Val Arg Arg Leu Thr Thr Asp Leu Arg Arg Ala Lys Gln Arg Leu Arg

35 40 45

Glu Ala Glu Asp Arg Ala His Glu Pro Ile Ala Ile Val Gly Met Ala

50 55 60

Cys Arg Tyr Pro Gly Gly Val Gly Ser Pro Glu Asp Leu Trp Arg Leu

65 70 75 80

Val Leu Asp Gly Arg Asp Ala Met Gly Ala Phe Pro Thr Asp Arg Gly

85 90 95

Trp Asp Leu Ala Ala Leu Phe Ala Asp Asp Pro Glu Arg Ser Gly Thr

100 105 110

Ser His Thr Arg Glu Gly Gly Phe Leu His Asp Ala Gly Glu Phe Asp

115 120 125

Pro Gly Leu Phe Gly Ile Ser Pro Arg Glu Ala Leu Ala Met Asp Pro

130 135 140

Gln Gln Arg Leu Leu Leu Glu Thr Ala Trp Glu Ala Val Glu Arg Ala

145 150 155 160

Gly Ile Asp Pro Thr Ser Leu Arg Gly Ser Arg Thr Gly Val Tyr Ala

165 170 175

Gly Val Met Tyr His Asp Tyr Gly Thr Gly Ala Asp Pro Leu Pro Glu

180 185 190

Gly Val Glu Gly Tyr Leu Gly Leu Gly Thr Ala Gly Ser Val Ala Ser

195 200 205

Gly Arg Ile Ala Tyr Thr Leu Gly Leu Glu Gly Pro Ala Val Thr Val

210 215 220

Asp Thr Ala Cys Ser Ser Ser Leu Val Ala Leu His Ser Ala Val Arg

225 230 235 240

Ala Leu Arg Ala Gly Glu Cys Thr Met Ala Leu Ala Gly Gly Ala Thr

245 250 255

Val Leu Ser Thr Pro Ala Val Phe Val Asp Phe Ser Arg Gln Gly Gly

260 265 270

Leu Ala Ala Asp Gly Arg Cys Lys Ser Tyr Ser Ala Glu Ala Asp Gly

275 280 285

Thr Gly Trp Ser Glu Gly Val Gly Met Leu Leu Val Glu Arg Leu Gly

290 295 300

Asp Ala Glu Arg Leu Gly His Pro Val Leu Ala Val Leu Arg Gly Ser

305 310 315 320

Ala Val Asn Gln Asp Gly Ala Ser Ser Gly Leu Thr Thr Pro Asn Gly

325 330 335

Pro Ala Gln Gln Arg Val Ile Arg Gln Ala Leu Ala Asp Ala Arg Leu

340 345 350

Thr Pro Ala Asp Leu Asp Leu Val Glu Gly His Gly Thr Gly Thr Pro

355 360 365

Leu Gly Asp Pro Ile Glu Val Gln Ala Leu Leu Ala Thr Tyr Gly Gln

370 375 380

Asp Arg Ala Glu Pro Leu Trp Leu Gly Ser Val Lys Ser Asn Ile Gly

385 390 395 400

His Thr Gln Ala Ala Ala Gly Val Ala Gly Val Ile Lys Ala Val Leu

405 410 415

Ala Leu Arg His Gly Val Leu Pro Gly Thr Ala His Leu Thr Glu Pro

420 425 430

Thr Pro Gln Val Asp Trp Thr Ala Gly Ala Val Glu Pro Leu Arg Glu

435 440 445

Thr Arg Ala Trp Pro Glu Thr Gly Arg Pro Arg Arg Ala Ala Val Ser

450 455 460

Ser Phe Gly Ile Ser Gly Thr Asn Ala His Ile Val Leu Glu Gln Ala

465 470 475 480

Pro Ala Pro Ala Ala Pro Gln Ala Ala Gly Ala Gln Ala Pro Ala Ala

485 490 495

Pro Arg Pro Val Gly Asn Gln Ala Thr Ala Ala Pro Arg Ser Met Glu

500 505 510

Asp Arg Thr Ala Ala Ala Pro Ser Ala Gly Gly Asp Pro Thr Leu Thr

515 520 525

Ala Pro Ala Pro Ser Ala Pro Arg Pro Ala Pro Ala Ala Leu Pro Val

530 535 540

Pro Leu Ser Ala Ala Thr Glu Pro Gly Val Arg Ala Gln Ala Leu Arg

545 550 555 560

Leu Ala Ala His Leu Thr Glu His Pro Glu Leu Ala Pro Gln Asp Ile

565 570 575

Ala Phe Ser Ala Ala Thr Thr Arg Ala Ala Leu Ala Ser Arg Ala Val

580 585 590

Val Leu Ala Asp Asp Arg Ala Gly Leu Leu Asp Ala Leu Thr Ala Leu

595 600 605

Ala Glu Gly Arg Pro Gly Pro Ala Val Val Thr Gly Ala Ala Ala Ala

610 615 620

Gly Ala Arg Arg Ile Thr Phe Val Phe Pro Gly Gln Gly Ala Gln Trp

625 630 635 640

Ala Gly Met Ala Val Pro Leu Leu Glu Thr Ser Pro Val Phe Ala Ala

645 650 655

Lys Trp Ala Glu Cys Ala Arg Val Leu Ala Pro Trp Val Asp Trp Ser

660 665 670

Pro Asp Glu Ala Leu Arg Ser Pro Gln Ala Leu Glu Arg Val Asp Val

675 680 685

Val Gln Pro Val Leu Trp Ala Val Met Val Ser Leu Ala Glu Leu Trp

690 695 700

Arg Ala Ala Gly Val Arg Pro Asp Ala Val Leu Gly His Ser Gln Gly

705 710 715 720

Glu Ile Ala Ala Ala Cys Val Ala Gly Ala Leu Ser Leu Glu Asp Gly

725 730 735

Ala Lys Val Val Ala Leu Arg Ala Lys Ala Leu Leu Ala Leu Ala Gly

740 745 750

Arg Gly Gly Met Leu Ser Val Pro Leu Pro Glu Ala Glu Val Arg Ala

755 760 765

Arg Leu Asp Ser Arg Pro Gly Leu Gly Ile Ala Ala Val Asn Gly Pro

770 775 780

Ala Thr Val Val Val Ser Gly Glu Thr Ala Ala Leu Asp Glu Ala Gln

785 790 795 800

Ala Ala Trp Glu Ala Glu Gly Val Arg Val Arg Arg Ile Pro Val Asp

805 810 815

Tyr Ala Ser His Ser Pro His Val Ala Glu Val Gln Asp Arg Leu Ala

820 825 830

Ala Asp Leu Ala Gly Ile Ala Pro Arg Pro Ala Glu Val Thr Phe Leu

835 840 845

Ser Thr Leu Thr Gly Glu Pro Phe Asp Thr Thr Gly Leu Asp Ala Gly

850 855 860

Tyr Trp Tyr Arg Asn Leu Arg Glu Gln Val Arg Phe Glu Ala Ala Thr

865 870 875 880

Arg Arg Ala Leu Glu Gln Gly His Arg Val Phe Ile Glu Val Gly Pro

885 890 895

His Pro Val Leu Thr Leu Gly Val Gln Gln Thr Ala Glu Ala Met Asp

900 905 910

Val Pro Ala Glu Ala Ile Ala Thr Leu Arg Arg Asp Gln Gly Asp Leu

915 920 925

Leu Arg Phe Arg Thr Ala Leu Ala Glu Ala Ala Val Leu Gly Ala Pro

930 935 940

Val Asp Trp Ala Ala Glu Leu Ala Pro Tyr Ala Pro Arg Arg Val Asp

945 950 955 960

Leu Pro Thr Tyr Ala Phe Gln Arg Glu Arg Tyr Trp Leu Thr Pro Gln

965 970 975

Arg Arg Ala Ala Leu Ala Ala Ser Thr Gly Thr Asp Pro Trp Asp Gly

980 985 990

Arg Phe Trp Asp Ile Val Asp Arg Ala Asp Ala Glu Glu Leu Ser Arg

995 1000 1005

Ala Leu Gly Val Asp Thr Asp Asp Pro Leu Thr Ala Val Val Pro Ala

1010 1015 1020

Leu Ala Arg Trp Arg Arg Leu Leu Arg Glu Arg Ser Ala Val Asp Ser

1025 1030 1035 1040

Trp Arg Tyr Thr Val Ala Trp Glu Arg Leu Ala Val Pro Asp Ser Ala

1045 1050 1055

Arg Leu Thr Gly Pro Trp Leu Leu Val Val Pro Glu Pro Arg Ala Gly

1060 1065 1070

Asp Ala Leu Ala Ala Arg Cys Ala Ala Ala Leu Thr Gly His Gly Ala

1075 1080 1085

Glu Val Thr Thr Leu Thr Leu Gly Ala Asp Asp Thr Asp Arg Ala Ala

1090 1095 1100

Leu Ala Ala Arg Leu Thr Gly Leu Arg Pro Ala Gly Val Leu Ser Leu

1105 1110 1115 1120

Leu Ala Leu Asp Asp Ala Pro His Pro Ala His Pro Ala Leu Pro Thr

1125 1130 1135

Gly Leu Ala Leu Thr Val Ala Leu Val Gln Ala Leu Gly Asp Ala Gly

1140 1145 1150

Val Thr Ala Pro Leu Trp Cys Ala Thr Arg Gly Ala Val Ala Thr Gly

1155 1160 1165

Pro Ala Asp Pro Val Thr Ala Pro Val Gln Ala Gln Ile Trp Gly Leu

1170 1175 1180

Gly Arg Val Val Ala Leu Glu His Pro Asp Arg Trp Gly Gly Leu Ile

1185 1190 1195 1200

Asp Leu Pro Ala Glu Trp Asp Asp Arg Ala Ala Gly Arg Leu Ala Ala

1205 1210 1215

Leu Leu Ala Ala Gly Gly Asp Glu Asp Gln Thr Val Leu Arg Ala Thr

1220 1225 1230

Gly Val Ser Ala Arg Arg Leu Thr Arg Ala Pro Leu Gly Thr Glu Pro

1235 1240 1245

Ala Pro Arg Pro Trp Arg Cys Ala Gly Thr Val Leu Leu Thr Gly Gly

1250 1255 1260

Thr Gly Ala Leu Gly Pro His Leu Val Arg Trp Leu Ala Gly Gln Gly

1265 1270 1275 1280

Ala Glu Arg Ile Val Leu Pro Gly Arg Arg Gly Ala Asp Ala Pro Ile

1285 1290 1295

Ala Ala Glu Leu Thr Ala Glu Leu Ala Gly Thr Gly Thr Glu Leu His

1300 1305 1310

Phe Pro Val Cys Asp Val Thr Asp Arg Ala Glu Leu Ala Ala Leu Ile

1315 1320 1325

Asp Gly Leu Asp Thr Ala Gly Thr Pro Val Arg Ser Val Leu His Ala

1330 1335 1340

Ala Thr Ala Leu Glu Leu Arg Pro Leu Ala Asp Thr Pro Val Asp Thr

1345 1350 1355 1360

Phe Ala Gly Gln Thr Ala Ala Lys Val Leu Gly Ala Arg His Leu Asp

1365 1370 1375

Glu Leu Phe Ala Asp Arg Asp Leu Asp Ala Phe Val Leu Phe Ser Ser

1380 1385 1390

Val Ala Gly Val Trp Gly Ser Gly Leu His Ala Pro Tyr Ala Ala Ala

1395 1400 1405

Asn Ala Tyr Leu Asp Ala Leu Ala Glu Asp Arg Arg Ala Arg Gly Leu

1410 1415 1420

Thr Ala Thr Ser Val Ala Trp Gly Ile Trp Ala Ala Val Asn Glu Trp

1425 1430 1435 1440

Asp Gly Val Asn Ala Asp Val Asp Pro Glu Arg Val Gly Arg Gln Gly

1445 1450 1455

Leu Pro Phe Leu Asp Pro Asp Leu Ala Val Ala Gly Leu Arg Gln Ala

1460 1465 1470

Leu Asp His Asp Glu Thr Thr Val Val Ile Ala Asp Val Asp Trp Thr

1475 1480 1485

Arg Phe Val Pro Val Phe Cys Ser Ala Arg Arg Arg Pro Leu Leu Glu

1490 1495 1500

Ser Val Pro Glu Ala Ala Ala Val Leu Arg Thr Ala Thr Val Asp Thr

1505 1510 1515 1520

Gly Thr Val Ser Ala Leu Arg Glu Arg Leu Gly Ser Leu Pro Glu Ala

1525 1530 1535

Gly Arg Arg Arg Ala Val Thr Glu Leu Val Arg Glu His Ala Ala Ala

1540 1545 1550

Val Leu Gly His Asp Ser Pro Ala Ala Leu Pro Ala Asp Arg Ala Phe

1555 1560 1565

Arg Asp Val Gly Phe Asp Ser Ile Thr Ala Val Glu Leu Arg Asn Arg

1570 1575 1580

Leu Arg Ser Ala Thr Gly Leu Ala Leu Pro Ala Thr Leu Val Phe Asp

1585 1590 1595 1600

His Pro Ser Pro Thr Ala Leu Ala Gly His Leu Leu Ala Leu Ala Phe

1605 1610 1615

Asp Thr Ala Ala Ala Asp Leu Ala Ala Pro Ala Ala Arg Ala Ala Asp

1620 1625 1630

Asp Asp Asp Asp Pro Ile Ala Val Val Gly Leu Ser Cys Arg Tyr Ala

1635 1640 1645

Gly Gly Val Ala Ser Pro Asp Glu Leu Trp Arg Leu Val Val Ala Gly

1650 1655 1660

Gln Asp Ala Val Gly Ala Leu Pro Thr Asp Arg Gly Trp Asp Leu Asp

1665 1670 1675 1680

Ser Leu Tyr Asp Ser Asp Pro Asp Ala Arg Gly Arg Ser Tyr Val Arg

1685 1690 1695

Gln Gly Ala Phe Leu Thr Asp Pro Ala Gly Phe Asp Ala Ala Phe Phe

1700 1705 1710

Gly Ile Ala Pro Ala Glu Ala Arg Ala Thr Asp Pro Gln Gln Arg Leu

1715 1720 1725

Leu Leu Glu Ala Ala Trp Glu Ala Phe Glu His Ala Gly Ile Asp Ala

1730 1735 1740

Thr Gly Leu Arg Gly Ser Arg Val Gly Val Phe Ala Gly Ala Asn Val

1745 1750 1755 1760

Gly Asp Tyr Ala Ser Ser Arg Gly Pro Gly Ala Gly Gly Ser Asp Gly

1765 1770 1775

Gln Leu Leu Thr Gly Asn Val Pro Ser Val Ile Ser Gly Arg Ile Ser

1780 1785 1790

Tyr Thr Phe Gly Phe Glu Gly Pro Ala Val Thr Val Asp Thr Ala Cys

1795 1800 1805

Ser Ser Ala Leu Val Ala Leu His Leu Ala Cys Arg Ser Val Arg Gly

1810 1815 1820

Gly Glu Ser Asp Met Ala Leu Ala Gly Gly Val Ala Leu Met Ser Ser

1825 1830 1835 1840

Pro Ala Ala Leu Ile Gly Phe Ser Ala Gln Arg Gly Leu Ser Gly Asp

1845 1850 1855

Gly Arg Cys Lys Ala Phe Ala Asp Ala Ala Asp Gly Thr Gly Leu Ala

1860 1865 1870

Glu Gly Val Gly Leu Leu Leu Val Glu Arg Leu Ser Arg Ala Arg Ala

1875 1880 1885

Gln Gly His Arg Val Leu Ala Leu Val Arg Gly Ser Ala Ile Asn Gln

1890 1895 1900

Asp Gly Ala Ser Asn Gly Leu Thr Ala Pro Ser Gly Pro Ala Gln Gln

1905 1910 1915 1920

Arg Val Ile Thr Ala Ala Leu Ala Asp Ala Gly Leu Arg Pro Ala Asp

1925 1930 1935

Val Asp Ala Val Glu Ala His Gly Thr Gly Thr Arg Leu Gly Asp Pro

1940 1945 1950

Ile Glu Ala Gln Ala Leu Leu Ala Thr Tyr Gly Gln Asp Arg Ala Glu

1955 1960 1965

Pro Leu Trp Leu Gly Ser Val Lys Ser Asn Ile Gly His Ser Gln Ala

1970 1975 1980

Ala Ser Gly Ala Ala Gly Val Ile Lys Thr Val Gln Ala Leu Arg His

1985 1990 1995 2000

Gly Leu Leu Pro Ala Thr Leu His Val Asp Arg Pro Thr Thr Gln Val

2005 2010 2015

Asp Trp Thr Ala Gly Ala Val Glu Val Leu Thr Glu Ala Arg Asp Trp

2020 2025 2030

Pro Ala Val Asp Arg Pro Arg Arg Ala Ala Val Ser Ala Phe Gly Leu

2035 2040 2045

Ser Gly Thr Asn Ala His Val Ile Leu Glu Gln Ala Pro Ala Glu Asp

2050 2055 2060

Ala His Pro Ala Pro Glu Pro Ala Pro Gly Glu Asp Ser His Pro Thr

2065 2070 2075 2080

Pro Glu Thr Ala Pro Gly Glu Asp Ala Pro Arg Thr Ala Pro Glu Pro

2085 2090 2095

Ala Arg Pro Val Val Trp Pro Val His Gly Arg Thr Arg Asp Ala Leu

2100 2105 2110

Arg Ala Gln Ala Ala Arg Leu Arg Thr His Leu Glu Thr Arg Pro Asp

2115 2120 2125

Ala Arg Pro Ala Asp Val Gly Trp Thr Leu Ala Ala Gly Arg Ala Val

2130 2135 2140

Phe Asp His Arg Ala Val Val Leu Gly Ala Asp Arg Ala Glu Leu Leu

2145 2150 2155 2160

Arg Gly Leu Asp Ala Val Ala Ala Gly Thr Pro Asp Pro Ala Val Ala

2165 2170 2175

Asp Gly Ala Ala Gln Gly Ala Asp Arg Ala Val Phe Val Phe Pro Gly

2180 2185 2190

His Gly Ala Gln Trp Pro Gly Met Ala Arg Arg Leu Phe Asp Asp Phe

2195 2200 2205

Pro Val Phe Arg Glu Ser Val Leu Gln Cys Ala Asp Ala Phe Ala Glu

2210 2215 2220

Phe Val Asp Trp Ser Leu Leu Asp Val Leu Arg Asp Glu Glu Gly Ala

2225 2230 2235 2240

Pro Pro Leu His Arg Val Asp Val Val Gln Pro Ala Leu Phe Thr Met

2245 2250 2255

Met Val Ser Leu Ala Ala Leu Trp Arg Ser Tyr Gly Val Glu Pro Ser

2260 2265 2270

Ala Val Val Gly His Ser Gln Gly Glu Ile Ala Ala Ala Tyr Val Ala

2275 2280 2285

Gly Ala Leu Asp Leu Arg Asp Ala Ala Arg Ile Val Ala Thr Arg Gly

2290 2295 2300

Lys Ala Trp Leu Thr Leu Ala Gly Thr Gly Gly Met Ala Ser Val Ala

2305 2310 2315 2320

Leu Pro Arg Ala Glu Ala Ala Glu Arg Leu Arg Pro Phe Gly His Arg

2325 2330 2335

Leu Asp Ile Ala Ala Val Asn Asp Pro Arg Ser Val Thr Val Ala Gly

2340 2345 2350

Asp Leu Asp Ala Leu Glu Glu Phe Leu Thr Gly Leu Glu Thr Glu Gly

2355 2360 2365

Val Arg Val Arg Arg Val Arg Gln Ile Val Gly Ala Gly His Thr Ala

2370 2375 2380

His Val Asp Ala Leu Arg Asp Gln Leu Ile Glu Thr Leu Ala Pro Thr

2385 2390 2395 2400

Ala Pro Arg Ser Ala Pro Ile Ala Phe Cys Ser Thr Val Thr Gly Gly

2405 2410 2415

Leu Leu Asp Thr Ala Gly Leu Asp His His Tyr Trp Tyr Arg Asn Ala

2420 2425 2430

Arg Arg Thr Val Leu Phe Glu Gln Ala Val Arg Thr Leu Ala Glu Gln

2435 2440 2445

Gly Tyr Gly Pro Phe Leu Glu Ile Ser Ala His Pro Met Phe Thr Val

2450 2455 2460

Ala Val Gln Glu Thr Leu Glu Asp Ala Gly Val Gly Ala Ala Val Leu

2465 2470 2475 2480

Ala Thr Leu Arg Arg Asp Glu Gly Gly Pro Asp Arg Phe Leu Arg Ala

2485 2490 2495

Ala Ala Glu Ala His Thr Ala Gly Val Thr Val Asp Trp Arg Pro Ala

2500 2505 2510

Phe Ala Gly Ala Gly Ala Arg Thr Thr Asp Leu Pro Thr Tyr Ala Phe

2515 2520 2525

Gln Arg Thr Arg His Trp Leu Glu Pro Gly Gly Asp Ser Gly Asp Val

2530 2535 2540

Thr Ala Ala Gly Leu Ala Pro Ala Gly His Pro Leu Leu Gly Ala Val

2545 2550 2555 2560

Val Glu Leu Ala Asp Gly Ala Met Val Leu Thr Gly Arg Leu Ser Ala

2565 2570 2575

Thr Ala Gln Pro Trp Pro Gly Pro Glu Ala Pro Asp Ala Ala Leu Val

2580 2585 2590

Asp Leu Val Leu Ala Ala Ala Asp Arg Ala Asp Cys Ala Gly Val Gly

2595 2600 2605

Glu Leu Thr Val Tyr Glu Pro Leu Met Ala Pro Ala Gly Gly Ala Asp

2610 2615 2620

Thr Arg Val Thr Val Gly Ala Pro Asp Ala Val Gly Ser Arg Thr Ala

2625 2630 2635 2640

Ala Val His Thr Arg Thr Ala Asp Ser Pro Trp Val Arg His Ala Glu

2645 2650 2655

Ala Thr Leu Val Ala Arg Pro Ala Pro Gly Glu Ser Leu Thr Asp Trp

2660 2665 2670

Pro Pro Ala Gly Ala Glu Pro Ile Asp Pro Pro Ala Glu Ala Gly Arg

2675 2680 2685

Ala Gly Val Ala Val Thr Ala Ala Trp Gln Arg Gly Asp Asp Leu Phe

2690 2695 2700

Thr Glu Val Ala Leu Asp Asp Gly Ala Ala Glu Arg Ala Asp Ala Phe

2705 2710 2715 2720

Ala Leu His Pro Leu Leu Leu Asp Ala Ala Leu Ser Pro Leu Leu Asp

2725 2730 2735

Gly Glu Leu Thr Pro Thr Ala Trp Ser Gly Val Arg Leu His Ala Thr

2740 2745 2750

Gly Ala Arg Thr Leu Arg Ala Arg Ala Glu Arg Ile Gly Pro Asp Thr

2755 2760 2765

Val Ala Leu Thr Leu Ala Asp Pro Glu Gly Gly Pro Val Leu Thr Ala

2770 2775 2780

Asp Thr Val Arg Leu Ala Ala Ala Pro Ala Ala Gly Ala Gly Arg Arg

2785 2790 2795 2800

Thr Gly Lys Asp Ala Leu Phe Arg Met Glu Trp Val Pro Ala Pro Leu

2805 2810 2815

Ala Pro Ala Thr Pro Gly Arg Trp Ala Val Leu Gly Ala Asp Pro Leu

2820 2825 2830

Gly Ala Ala Asp Thr Leu Arg Ser Leu Gly His Thr Val His Gly Ala

2835 2840 2845

Asp Gly Pro Ala Gly Leu Thr Glu Val Pro Asp Ala Val Leu Ile Thr

2850 2855 2860

Ala Val Gly Ala Pro Gly Glu Ala Pro Ala Glu Ala Arg Thr Val Leu

2865 2870 2875 2880

His Gly Thr Leu Ala Ala Leu Gln Thr Leu Leu Ala Asp Asp Arg Phe

2885 2890 2895

Thr Ala Val Pro Leu Val Val Leu Thr Arg Gly Ala Ala Ala Asp Arg

2900 2905 2910

Ala Asp Asp Leu Ala Gly Ala Ala Ala Trp Gly Leu Val Arg Ser Ala

2915 2920 2925

Gln Ser Glu His Pro Gly Arg Phe Val Leu Ala Asp Ile Asp Asp Asp

2930 2935 2940

Pro Arg Ser Trp Arg Ala Leu Gly Gly Val Pro Ala Thr Gly Glu Pro

2945 2950 2955 2960

Gln Leu Ala Leu Arg Ala Gly Ala Ala Thr Val Pro Arg Leu Ala Arg

2965 2970 2975

Leu Ala Pro Pro Asp Ala Pro Ala Pro Trp Asp Pro Asp Arg Thr Val

2980 2985 2990

Leu Val Thr Gly Ala Ser Gly Asp Leu Gly Ala Leu Val Ala Arg His

2995 3000 3005

Leu Val Ala Ala His Gly Val Arg His Leu Ile Leu Ala Ser Arg Arg

3010 3015 3020

Gly Pro Ala Ala Pro Gly Ala Ala Gly Leu Gly Ala Glu Leu Arg Ala

3025 3030 3035 3040

Ser Gly Ala Ala Thr Val Thr Ile Ala Ala Cys Asp Thr Ala Asp Arg

3045 3050 3055

Lys Ala Leu Ala Glu Leu Ile Ala Ala Val Pro Asp Glu His Pro Leu

3060 3065 3070

Thr Ala Val Val His Ser Ala Ser Val Leu Asp Asp Gly Val Ile Ala

3075 3080 3085

Ala Leu Asp Pro Asp Arg Leu Asp Thr Ala Leu Arg Pro Lys Ala Asp

3090 3095 3100

Ala Ala Trp His Leu His Glu Leu Thr Arg His Leu Asn Leu Ser Ala

3105 3110 3115 3120

Phe Val Leu Phe Ser Ser Val Ala Gly Thr Phe Gly Gly Leu Gly Gln

3125 3130 3135

Gly Asn Tyr Ala Ala Gly Asn Ala Phe Leu Asp Ala Leu Ala Arg His

3140 3145 3150

Arg Arg Ala His Gly Leu Pro Ala Thr Ser Val Ala Trp Gly Trp Trp

3155 3160 3165

Ala Glu Arg Ala Ala Lys Ser Gly His Gly Thr Ala Ala Glu Ala Pro

3170 3175 3180

Val Ala Val Asn Gly Met Thr Pro Leu Thr Glu Asp His Gly Leu Ala

3185 3190 3195 3200

Leu Phe Asp Ala Ala Cys Arg Gly Asp Glu Pro Phe Val Val Ala Gly

3205 3210 3215

Ala Leu His Leu Arg Ser Leu Arg Ala Ala Ala Asp Glu Leu Pro Ala

3220 3225 3230

Pro Leu Arg Gly Leu Val Arg Ala Pro Ala Arg Lys Ala Ala Ala Gln

3235 3240 3245

Ala Ala Gly Thr Gly Thr Pro Thr Val Ala Gly Glu Leu Ala Gly Arg

3250 3255 3260

Pro Pro Ala Arg Arg Glu Ala Phe Leu Val Asp Leu Val Arg Asp Glu

3265 3270 3275 3280

Thr Ala Leu Val Leu Gly His Thr Gly Arg Asp Asp Val Pro Ala His

3285 3290 3295

His Arg Phe Leu Asp Gln Gly Phe Asp Ser Leu Ala Ala Leu Lys Leu

3300 3305 3310

Arg Asn Arg Leu Ala Ala Ala Thr Gly Leu Arg Leu Pro Pro Thr Leu

3315 3320 3325

Val Phe Asp His Pro Thr Pro Thr Glu Leu Ala Arg His Leu Leu Ala

3330 3335 3340

Glu Leu Val Pro Pro Ala Asp Ala Glu Pro Pro Ala Phe Asp Asp Glu

3345 3350 3355 3360

Asp Ala Ala Thr Ala Val Leu Val Leu Glu Glu Leu Gly Gln Leu Asp

3365 3370 3375

Glu Ala Ile Thr Arg Leu Pro Ala Asp Gly Pro Glu Arg Thr Arg Ile

3380 3385 3390

Ala Gly Leu Leu Thr Asp Leu Leu Ala Arg Trp Gly Arg ter

3395 3400 3405

<210>13

<211>388

<212>PRT

<213>Streptomyces flaveolus

<400>1

Met Glu Asn Val Gln Ala Trp Val Leu Pro Ala Gly Pro Gln Asp Arg

1 5 10 15

Ser Gly Gly Pro Val Arg Gly Glu Leu Thr Leu Thr Arg Ile Pro Val

20 25 30

Ala Ser Pro Gly Glu His Glu Val Leu Val Glu Ser Leu Val Gly Cys

35 40 45

Trp Glu Ala Asn Met Glu His Ala Leu Ala Arg Ser Pro Val Asp Ile

50 55 60

Val Arg Gln Arg Gly Glu Glu Gln Ile Val Ile Gly Thr Cys Gly Val

65 70 75 80

Val Arg Val Leu Ser Thr Gly Ser Ala Val Arg Gly Leu Arg Glu Gly

85 90 95

Gln Glu Cys Leu Trp Ile Pro Phe Gly His Ile Asp Arg Asn Gly Tyr

100 105 110

Ala Glu Thr Ile Cys Ala Tyr Asp Ala Pro Gly Ser Pro Gly Leu Leu

115 120 125

Ala Glu Arg Met Val Val Pro Ala Asp Arg Leu Val Pro Leu Pro Ala

130 135 140

Asp Gly Arg Tyr Pro Leu Glu Arg Trp Ala Pro Phe Ala Arg Tyr Phe

145 150 155 160

Thr Ala Trp Asp Asn Trp Arg Val Thr Ser Arg Cys Trp Arg Ser Gln

165 170 175

Val Asp Asp Ala Trp Asp Glu Gln Pro Leu Val Leu Gly Trp Gly Gly

180 185 190

Gly Val Val Phe Ala Glu Leu Glu Leu Ala Arg Arg Glu Gly Phe Arg

195 200 205

Thr Ala Met Val Ser Gly Arg Glu Ser Arg Leu Lys Glu Ile Ala Ala

210 215 220

Ser Gly Ala Ile Ala Val Asp Arg Arg Glu Phe Arg Asp Leu Asp Tyr

225 230 235 240

Leu Arg Ala Lys Glu Ser Thr Asp Pro Asp Ala Met Asp Arg Tyr Arg

245 250 255

Ala Ser Glu Ala Arg Phe Leu Glu Ile Val Gly Glu Leu Ser Gly Gly

260 265 270

His Gly Val Ser Val Phe Leu Asp Asn Leu Gly Gly Gly Leu His Arg

275 280 285

Ala Thr Met Lys Ser Leu Ala Arg Glu Gly Val Val Ser Thr Val Gly

290 295 300

Trp Lys Thr Gly Met Arg Leu Trp Asn Leu Arg Ala Thr Glu Cys Ile

305 310 315 320

Ser Arg His Ile His Val His Thr His Ala Trp Arg His Gln Asp Ala

325 330 335

Pro Arg Ile Arg Asp Val Met Gln Glu Thr Gly Trp Leu Pro Asp Ile

340 345 350

Ala Asp Asp Pro Val Thr Ala Phe Ala Arg Val Pro Glu Leu Ala Asp

355 360 365

Ser Tyr Arg Arg Asp Asp Leu Asp Thr Tyr Phe Pro Leu Phe Ser Gly

370 375 380

Ala Gly Arg Gly

385

<210>14

<211>1515

<212>PRT

<213>Streptomyces flaveolus

<400>1

Met Ser Pro Arg Ser Tyr Asp Val Ala Val Val Gly Met Ala Cys Ala

1 5 10 15

Phe Pro Gly Ala Pro Asp Leu Asp Arg Tyr Trp Ala Asn Val Val Ala

20 25 30

Gly Val Asp Ser Val Thr Glu Val Pro Pro Asp Arg Trp Pro Ala Asp

35 40 45

Arg His Trp Gly Gly Ala Asp Ala Ala Pro Gly Glu Gln Ser Pro Ser

50 55 60

Lys Trp Gly Gly Phe Leu Pro Pro Leu Pro Phe Asp Ala Leu Ala His

65 70 75 80

Gly Val Pro Pro Asn Ser Leu Gly Gly Ile Glu Thr Ala Gln Leu Leu

85 90 95

Ala Leu His Thr Ala Asp Arg Ala Leu Ala Asp Ala Gly Tyr Pro Glu

100 105 110

Arg Pro Phe Asp Arg Glu Thr Thr Ser Val Ile Phe Ala Ala Glu Ala

115 120 125

Gly Ala Asp Leu Ala Ser Ala Tyr Ala Ala Arg Ser Met Leu Gly Gln

130 135 140

His Leu Glu Ser Val Pro Glu Glu Leu Asp Ala Arg Leu Pro Arg Leu

145 150 155 160

Thr Glu Asp Ser Phe Pro Gly Thr Leu Ala Asn Val Ile Ala Gly Arg

165 170 175

Ile Ala Asn Arg Leu Asp Leu Gly Gly Pro Thr Tyr Thr Val Asp Ala

180 185 190

Ala Cys Ala Ser Ser Leu Ala Ala Leu Asp Gln Ala Cys Lys Glu Leu

195 200 205

Leu Ala Glu Thr Ser Asp Met Val Leu Cys Gly Ala Val Asp Thr His

210 215 220

Asn Ala Leu His Asp His Leu Leu Phe Gly Ser Ala Arg Ala Leu Ser

225 230 235 240

Pro Thr Gly Arg Cys Arg Ala Phe Asp Ala Ser Ala Asp Gly Ile Val

245 250 255

Leu Ala Glu Gly Val Ala Cys Leu Val Leu Lys Arg Leu Ala Asp Ala

260 265 270

Glu Arg Asp Gly Asp Arg Val Tyr Ala Val Ile Lys Ala Val Gly Ala

275 280 285

Gly Ser Asp Gly Arg Gly Leu Gly Leu Thr Ala Pro Arg Pro Glu Gly

290 295 300

Gln Arg Arg Ala Leu Glu Arg Ala Tyr Ala Leu Ala Gly Leu Ser Pro

305 310 315 320

Arg Glu Val Gly Leu Val Glu Ala His Gly Thr Gly Thr Val Leu Gly

325 330 335

Asp Arg Thr Glu Leu Glu Thr Leu Thr Glu Val Phe Arg Thr Ala Gly

340 345 350

Ala Gly Pro Gly Ala Cys Ser Leu Gly Ser Val Lys Ser Leu Ile Gly

355 360 365

His Ala Lys Cys Ala Ala Gly Met Ala Gly Leu Ile Lys Ala Val Leu

370 375 380

Ala Val His Thr Gly Val Arg Pro Pro Thr Arg Leu Thr Glu Pro Asn

385 390 395 400

Ala Ala Trp Asp Pro Ala Thr Ser Pro Phe Ala Phe Asp Thr Thr Ala

405 410 415

Arg Pro Trp Ala Glu Pro Arg Arg Val Ala Gly Val Ser Ala Phe Gly

420 425 430

Phe Gly Gly Val Asn Tyr His Ala Val Val Ala Ala Pro Ala Pro Asp

435 440 445

Pro Ser His Glu Ser Ala Pro Arg Ser Ala Gly Leu Leu Leu Phe Arg

450 455 460

Gly Thr Glu Asp Glu Val Gln Thr Ala Leu Arg Asp Leu Ala Gly Arg

465 470 475 480

Leu Ala Glu Gly Thr Val Arg Pro Ser Ala Leu Thr Gly Val Thr Gly

485 490 495

Ala Gly Pro Ala Arg Val Ala Leu Val Thr Glu Gly Ala Ala Thr Leu

500 505 510

Gly Glu Gln Leu Asp Leu Ala Leu Arg Leu Glu Asp Arg Pro Glu Arg

515 520 525

Gly Val His Val Gly Asp Gly Ala Arg Gln Pro Val Ala Leu Leu Phe

530 535 540

Pro Gly Gln Gly Ser Gln Arg Pro Gly Met Leu Ala Asp Leu Phe Val

545 550 555 560

Ala Phe Pro Arg Leu His Arg Phe Leu Arg Ala Glu Pro Ala Leu Thr

565 570 575

Gly Ala Leu Phe Pro Pro Ala Ala Phe Gly Gly Thr Pro Pro Arg Leu

580 585 590

Val Asp Thr Arg Leu Ala Gln Pro Ala Leu Gly Leu Gly Ala Leu Ala

595 600 605

Ala Leu Asp Leu Leu Arg Ala Cys Gly Val Glu Pro Asp Met Thr Ala

610 615 620

Gly His Ser Tyr Gly Glu Leu Pro Ala Leu Ala Ala Ala Gly Ala Leu

625 630 635 640

Pro Glu Thr Ala Val Met Pro Leu Ser Gly Ala Arg Ala Glu Ala Met

645 650 655

His Thr Ala Ala Gly Gln Asp Pro Gly Ala Met Ala Ala Val Ala Ala

660 665 670

Pro Ala Gly Thr Val Ala Glu Leu Leu Ala Arg His Gly Leu Ala Asp

675 680 685

Asp Val Thr Pro Ala Asn Arg Asn Ala Pro Gly Gln Val Val Leu Ala

690 695 700

Gly Ser Ala Ala Gly Ile Asp Ala Ala Val Ala Val Leu Arg Glu Ala

705 710 715 720

Gly His Thr Ala Lys Arg Leu Pro Val Ala Ala Ala Phe His Ser Pro

725 730 735

Arg Met Ala Pro Ala Val Asp Ala Phe Ala Gly Ala Leu Ala Gly His

740 745 750

Asp Leu Arg Ala Pro Arg Leu Pro Val Trp Ser Gly Ala Thr Ala Arg

755 760 765

Pro Tyr Pro Ala Asp Pro Glu Ala Ile Arg Thr Gln Leu Ala Gly Ala

770 775 780

Leu Ala Arg Pro Val Arg Phe Ala Glu Gln Ile Glu Asp Met Tyr Ala

785 790 795 800

Ala Gly Val Arg Val Phe Val Glu Ala Gly Pro Gly Thr Val Leu Thr

805 810 815

Arg Leu Ala Gly Glu Val Leu Ala Gly Arg Pro His Thr Ala Leu Ser

820 825 830

Val Glu Ala Pro Gly Arg Pro Gly Leu Ala His Leu Leu Gly Thr Leu

835 840 845

Ala Ala Leu Ala Val Arg Gly Thr Pro Val Arg Leu Asp Glu Leu Thr

850 855 860

Val Ala Arg Asp Glu Pro Val Ser Ala Pro Pro Gly Trp Thr Val Asp

865 870 875 880

Gly His Leu Val Arg Thr Ala Asp Gly Thr Pro Val Pro Gly Gly Leu

885 890 895

Gln Pro Ala Gly Pro Pro Val Thr Leu Thr Gly Gly Gly Val Pro Asp

900 905 910

Ser Arg Asp Glu Thr Val Ile Glu Phe Leu Arg Gly Thr Gln Arg Ile

915 920 925

Val Ala Ala Gln Gln Glu Val Met Leu Arg Tyr Leu Gly Thr Gln Pro

930 935 940

Pro Ala Pro Glu Pro Ala Pro Ala His Asp Pro Pro Glu Ser Ala Ala

945 950 955 960

Thr Ala Ala Pro Thr Val Leu Asp Leu Val Ala Ala Arg Thr Gly Tyr

965 970 975

Pro Pro Ala Met Leu Asp Pro Asp Leu Asp Leu Glu Ala Ala Leu Gly

980 985 990

Val Asp Ser Leu Lys Arg Thr Glu Ile Ala Ser Ala Leu Val Arg Gly

995 1000 1005

Thr Pro Ala Ala Asp Ala Val Gly Glu Leu Ala Asp Leu Arg Thr Ile

1010 1015 1020

Arg Ala Met Thr Asp Trp Leu Ala Thr Arg Thr Gly Pro Asp Gly Pro

1025 1030 1035 1040

Gly Pro Asp Gly Pro Gly Arg Gln Thr Ala Val Pro Glu Pro Glu Thr

1045 1050 1055

Met Pro Ala Gly Ala Ser Asp Thr Val Arg His Val Val Glu Pro Val

1060 1065 1070

Pro Glu Glu Pro Pro Ala Gly Pro Leu Pro Thr Leu Thr Arg Ala Ala

1075 1080 1085

Val Ser Gly Gly Gly Ala Val Gly Asp Val Leu Ala Thr Leu Leu Lys

1090 1095 1100

Glu Arg Gly Thr Glu Val Val Ser Asp Pro Ala Gly Cys Asp Ala Leu

1105 1110 1115 1120

Leu Leu Leu Asp Ala Leu Asp Gly Gly Asp Tyr Thr Leu Pro Gly Arg

1125 1130 1135

Phe Thr Asp Ile Arg Ala Ala Val Leu Gly Gly Leu Arg Thr Leu Leu

1140 1145 1150

Leu Ala Thr Cys His Asp Ala Gly Pro Ala Gly Ser Gly Val His Gly

1155 1160 1165

Leu Ala Arg Ala Leu Ser Arg Glu His Pro Gly Leu Ala Val Thr Ala

1170 1175 1180

Val Asp Leu Pro Ala Gly Gln Pro Ala Glu Glu Ala Ala Arg Thr Leu

1185 1190 1195 1200

Leu Ala Glu Leu Gly Gly Ser Arg Pro Ser Val Thr His Thr Asp Gly

1205 1210 1215

Arg Arg Ala Val Trp Arg Thr Arg Pro Ala Pro Leu Pro Ala Ala Asp

1220 1225 1230

Val Thr Ala Gly Asp Leu Gly Leu Asp Arg Asp Ser Val Val Leu Leu

1235 1240 1245

Thr Gly Gly Ala Arg Gly Ile Thr Ala Arg Val Ala Arg Ala Leu Ala

1250 1255 1260

Thr Leu Thr Gly Cys His Leu Glu Leu Val Gly Arg Ser Glu Pro Val

1265 1270 1275 1280

Thr Gly Thr Val Leu Thr Asp Ala Asp Leu Arg Thr Arg Leu Ile Ala

1285 1290 1295

Glu Gly Gly Arg Asp Pro Ala Gly Ile Glu Arg Ala Val Arg Ala His

1300 1305 1310

Ala Ala Gly Arg Gln Val Arg Gln Cys Leu Asp Asp Leu Ala Gly Pro

1315 1320 1325

Ala Ala Ser Val Arg Tyr His Arg Ala Asp Val Arg Asp Pro Glu Arg

1330 1335 1340

Leu Gly Ala Val Leu Asp Asp Val Tyr Ala Arg His Gly Arg Leu Asp

1345 1350 1355 1360

Thr Val Val His Ala Ala Gly Gln Val Arg Asp Arg Leu Leu Arg Asp

1365 1370 1375

Lys Ser Pro Asp Glu Phe Ala Glu Val Tyr Asp Thr Lys Val Ala Gly

1380 1385 1390

Ala Arg Ala Leu Ala Ala Arg Leu Arg Pro Gly Leu Arg His Leu Val

1395 1400 1405

Leu Phe Gly Ser Ile Ala Gly Val Thr Gly Asn Arg Gly Gln Thr Asp

1410 1415 1420

Tyr Ala Ala Ala Asn Asp Ala Leu Asp Thr Met Ala Arg Gln Trp Ser

1425 1430 1435 1440

Gly Arg Val Ala Asp Arg Val Leu Ala Leu Asp Trp Gly Pro Trp Ala

1445 1450 1455

Ala Asp Ala Gly Gly Met Val Thr Ala Glu Leu Glu Arg Ala Tyr Ala

1460 1465 1470

Arg Asn Gly Ile Gly Leu Ile Asp Pro Asp Asp Gly Val Arg Ala Phe

1475 1480 1485

Leu Arg Glu Leu Ala Phe Gly Arg Asp Pro Gln Val Leu Leu Thr Val

1490 1495 1500

Gly Asp Pro Ala Gly Phe Gly Ser Ala Leu Asp

1505 1510 1515

<210>15

<211>425

<212>PRT

<213>streptomyces flaveolus

<400>1

Leu Thr Asp Ile Leu Leu Ala Ala Ala Ala Gly Pro Gly Ala Leu Gly

1 5 10 15

Ala Ala Leu Arg Ser Gly Arg Arg Gly Gly Ala Gly Pro Cys Arg Val

20 25 30

Ala Val Leu Asp Pro Thr Ala Glu Arg Ile Ala Gln Ala Leu Ala Leu

35 40 45

Ile Asp Arg Gly Glu Pro Trp His Gly Gly Asn Asp Ile Arg Phe Ala

50 55 60

Pro Arg Pro Gly Pro Ala Gly Arg Thr Val Leu Leu Phe Pro Gly Leu

65 70 75 80

Asp Ala Arg Thr Gly Pro Pro Pro Asp Asp Val Leu Arg Trp Leu Gly

85 90 95

Arg Pro Ala Gly Pro Ala Thr Gly Ala Ala Thr Leu Ala Glu Arg Thr

100 105 110

His Ala Thr Leu Arg Leu Asn Arg Val Leu His Ala Ala Leu Leu Arg

115 120 125

Ala Gly Ile Val Pro Asp Ala Met Ala Gly His Ser Ala Gly Glu Trp

130 135 140

Ser Ala Leu Phe Ala Ala Gly Leu Phe Pro Glu Thr Ala Phe Asp Glu

145 150 155 160

Phe Ala Arg Thr Met Gly Ala Ala Pro Val Pro Asp Val Val Phe Ala

165 170 175

Ala Val Gly Leu Pro Ala Glu Ala Ala Ala Arg Leu Thr Glu Gly Glu

180 185 190

Pro Arg Val Val Leu Ser His Asp Asn Gly Pro Asp Gln Ser Val Leu

195 200 205

Cys Gly Asp Glu Asp Ala Val Thr Arg Cys Leu Asp Arg Ile Ala Asp

210 215 220

Pro Glu Val Pro Arg Arg Val Leu Pro Phe Arg Ser Gly Phe His Ser

225 230 235 240

Pro Met Leu Ala Pro Tyr Leu Pro Gly Phe Ser Ala Val Phe Asp Arg

245 250 255

Val Pro Leu Ala Arg Pro Ala Val Pro Val Trp Ser Ala Thr Thr Val

260 265 270

Ala Pro Tyr Pro Asp Asp Glu Thr Glu Leu Arg Ala Leu Leu Gly Arg

275 280 285

His Leu Val Glu Pro Val Arg Phe Arg Ala Val Ile Glu Arg Leu Tyr

290 295 300

Glu Gln Gly Ala Arg Val Phe Val Gln Ala Gly Cys Gly Ser Leu Thr

305 310 315 320

Arg Phe Val Thr Ala Thr Leu Ala Gly Arg Pro His Leu Thr Val Ser

325 330 335

Ala Gly Ser Ala Thr Arg Pro Gln Leu Asp Gln Leu Arg Trp Ala Ala

340 345 350

Ala Ala Leu Trp Thr Ala Gly His Thr Pro Asp Leu Ala Ala Leu Gly

355 360 365

Leu Thr Gly Pro Ala Ala Asp Pro Val Pro Ser Thr Arg Ala Val Thr

370 375 380

Gly Ala His Pro Arg Thr Gly Ala Tyr Pro Arg Thr His Pro Val Ala

385 390 395 400

Gly Ala Tyr Glu Ala Ala Val Ala Ala Val Ser Ala Ser Val Glu Ser

405 410 415

Val Tyr Arg Thr Trp Lys Glu Arg Thr

420 425

<210>16

<211>239

<212>PRT

<213>Streptomyces flaveolus

<400>1

Met Ser Ser Arg Arg His Ala Val Val Thr Gly Ser Ser Arg Gly Ile

1 5 10 15

Gly Ala Ala Val Ala Ala Arg Leu Ala Arg Ala Gly Trp Ala Val Thr

20 25 30

Gly Trp Asp Arg Thr Pro Gly Asp Thr Thr Gly Leu Ala Asp Trp Arg

35 40 45

Glu Val Asp Val Ser Asp Ala Glu Ser Val Gln Ala Ala Ala Arg Glu

50 55 60

Val Thr Ala Thr Asp Leu Leu Val Asn Ser Ala Gly Val Gly Ala Ile

65 70 75 80

Ala Pro Ser Ala Glu Leu Lys Pro Arg Thr Trp Glu Arg Val Val Gly

85 90 95

Val Asn Leu Ser Gly Thr Phe Tyr Cys Ala Gln Ala Leu Phe Pro Ala

100 105 110

Leu Ser Ala Arg Arg Gly Leu Val Val Asn Leu Ala Ser Val Met Ala

115 120 125

His Arg Ala Val Pro Gly Arg Ala Ala Tyr Cys Ala Ser Lys Ala Gly

130 135 140

Val Val Met Leu Thr Glu Ala Leu Gly Val Glu Trp Ala Glu His Gly

145 150 155 160

Val Arg Val Val Ala Val Ser Pro Ala Tyr Val Arg Thr Pro Leu Val

165 170 175

Ala Glu Gly Phe Ala Asn Gly Asn Leu Asp Glu Ser Ala Ile Ala Ala

180 185 190

Arg Thr Pro Leu Gly Arg Leu Ala Glu Pro Glu Glu Ile Ala Asp Leu

195 200 205

Val Leu Ser Leu Thr Gly Asp Glu Phe Ala Tyr Leu Thr Ala Thr Thr

210 215 220

Leu Arg Phe Asp Gly Gly Trp Thr Ala Asp Gly Val Phe Pro Arg

225 230 235

<210>17

<211>340

<212>PRT

<213>Streptomyces flaveolus

<400>1

Met Pro Val Thr Asp Phe Gly Val Leu Ala Leu Ala His Ala Leu Gly

1 5 10 15

Asp Pro Arg Asp Val Ala Ala Thr Ala Ala Asp His Val Asp Asp Pro

20 25 30

Asp Arg Val Leu Leu Trp Gly Tyr Arg Gly Tyr His Arg Ala Pro Gln

35 40 45

Gly Thr Thr Ser Thr Ala Leu Ala Ala Arg Ala Ala Glu Lys Ala Leu

50 55 60

Ala Lys Ala Gly Val Asp Pro Arg Asp Leu Asp Leu Val Val Val Ala

65 70 75 80

Asp Ser Ser Val Pro Glu Tyr Leu Leu Trp Asp Thr Ser Ala Val Val

85 90 95

Ala Arg Ala Ile Gly Ala Gly Thr Ala Pro Thr Leu Leu Leu Thr Gln

100 105 110

Gly Cys Ala Ser Ala Val Thr Ala Phe Gln Gln Ile Ala Gly Ile Phe

115 120 125

Ala Thr Arg Pro Asp Ala Glu Thr Val Leu Leu Val Ala Val Asn Gln

130 135 140

Val Ser Glu Ala His Thr Asn Arg Met Arg Phe Asn Thr Leu Leu Gly

145 150 155 160

Ser Asp Gly Ala Ala Ala Ala Val Leu Arg Arg Gly His Asp Arg Leu

165 170 175

Arg Trp Leu Ala Thr Glu Gln Leu Thr Asp Pro Thr Tyr Ala Asp Phe

180 185 190

Tyr Arg Val Glu Tyr Gly Gly Ala Ala Val Pro His Ala Pro Glu Gly

195 200 205

Ala Gly Asn Leu Asp Val Asp Pro Leu Ser Leu Val Tyr Arg His Phe

210 215 220

Arg Arg Glu Pro Glu His Leu Ala Lys Phe Val Arg Thr Leu Asn Ser

225 230 235 240

Arg Val Arg Thr Val Phe Glu Asp Ala Cys Asp Gly Ala Gly Val Asp

245 250 255

Pro Gly Gln Val Lys Arg Phe Leu Phe Leu Asn Asp Asn Gln Asp Ser

260 265 270

Leu Ala Asp Val Ala Lys Ala Val Gly Val Pro Leu Asp Arg Thr Asn

275 280 285

Ala Glu Leu Ala Lys Asp Leu Gly His Cys Gly Gly Ala Asp Gln Leu

290 295 300

Ile Cys Leu Asp Thr Leu Leu Glu Arg Gly Glu Leu Ala Glu Gly Asp

305 310 315 320

Val Val Ala Leu Ala Gly Leu Ser Ile Gly Met His Trp Tyr Cys Thr

325 330 335

Leu Leu Thr Val

340

<210>18

<211>84

<212>PRT

<213>Streptomyces flaveolus

<400>1

Met Ala Glu Leu Thr Ile Gln Asp Leu Ile Asp Ile Phe Asn Arg Ser

1 5 10 15

Ile Gly Asp Gln Asp Pro Val Glu Pro Glu Gly Asp Pro Ser Asp Val

20 25 30

Thr Phe Ala Ala Leu Gly Phe Asp Ser Leu Thr Thr Leu Asn Ala Val

35 40 45

Arg Arg Ile Glu Arg Lys His Gly Val Glu Leu Gly Glu Asn Val Ile

50 55 60

Ser Glu Ala Arg Thr Pro Arg Gln Leu Leu Asp Arg Val Asn Ala Ala

65 70 75 80

Leu Ala Ala Ala

<210>19

<211>616

<212>PRT

<213>streptomyces flaveolus

<400>1

Met Cys Gly Ile Thr Gly Trp Val Ala Tyr Gly Asp Asp Leu Ala Arg

1 5 10 15

His Arg Ser Val Val Gln Ala Met Thr Asp Thr Met Ile Cys Arg Gly

20 25 30

Pro Asp Ala Glu Gly Val Trp Ile Glu Gly Pro Val Ala Leu Gly His

35 40 45

Arg Arg Leu Ser Ile Ile Asp Pro Ala Gly Gly Ala Gln Pro Met Val

50 55 60

Ala Thr Arg Asp Gly Arg Thr Leu Ala Ala Leu Thr Phe Cys Gly Glu

65 70 75 80

Ile Tyr Asn Phe Arg Glu Leu Arg Ala Glu Leu Ala Ala Leu Gly His

85 90 95

Thr Phe Arg Thr Asp Ser Asp Thr Glu Val Val Leu Asn Ala Tyr Leu

100 105 110

Gln Trp Gly Ala Asp Phe Ala Ala Arg Leu Asn Gly Met Phe Ala Ile

115 120 125

Gly Leu Trp Asp Ala Thr Thr Arg Glu Leu Leu Leu Val Arg Asp Pro

130 135 140

Ile Gly Val Lys Pro Leu Tyr Tyr Ala Arg Thr Ser Asp Gly Val Val

145 150 155 160

Phe Gly Ser Glu Pro Lys Ala Val Leu Ala His Arg Gly Val Pro Arg

165 170 175

Arg Val Asp Ala Gln Gly Leu Ala Glu Ile Leu Asp Met Val Arg Thr

180 185 190

Pro Glu Val Thr Pro Phe Thr Asp Leu Ser Glu Val Arg Pro Gly Gln

195 200 205

Val Val Arg Val Thr Glu Gly Gly Leu Thr Arg Thr Arg Tyr Trp Gln

210 215 220

Leu Thr Ala Arg Glu His Thr Asp Asp Leu Asp Thr Thr Ile Ala Thr

225 230 235 240

Val Arg Glu Leu Leu Glu Asp Ile Val Ser Arg Gln Leu Ile Ala Asp

245 250 255

Val Pro Val Ala Thr Leu Leu Ser Gly Gly Leu Asp Ser Ser Ala Ile

260 265 270

Thr Ala Leu Ala Gln Arg Thr Leu Thr Ala Glu Gly Arg Gly Pro Val

275 280 285

Arg Ser Tyr Ser Val Asp Phe Gln Gly Ala Ala Asp Gly Phe Glu Pro

290 295 300

Asp Ala Val Arg Gly Thr Ala Asp Ala Pro Tyr Val Arg Asp Cys Ala

305 310 315 320

Ala His Val Gly Ala Asp His Ser Glu Val Leu Leu Asp Ser Thr Glu

325 330 335

Leu Ala Asp Pro Ala Val Arg Ala Ala Ile Leu Gly Ala Thr Asp Leu

340 345 350

Pro Pro Ala Tyr Trp Gly Asp Leu Trp Pro Ser Leu Tyr Leu Leu Cys

355 360 365

Arg Glu Ile Arg Lys Arg Ala Thr Val Val Leu Ser Gly Glu Ser Ala

370 375 380

Asp Glu Leu Phe Gly Gly Tyr Arg Trp Tyr Gln Arg Pro Glu Ala Val

385 390 395 400

Asp Ala Pro Thr Phe Pro Trp Leu Thr Pro Gly Ser Ala Arg Ile Phe

405 410 415

Gly Gly Ser Ser Leu Ile Asp Gln Gly Leu Leu Glu Lys Leu Asp Leu

420 425 430

Glu Gly Tyr Arg Arg Asp Arg Tyr Ala Glu Ala Leu Ala Glu Val Pro

435 440 445

Val Leu Ala Gly Glu Ser Ala Val Asp Arg Arg Met Arg Glu Val Thr

450 455 460

Tyr Leu Asn Ile Thr Arg Phe Met Gln Ala Val Leu Asp Arg Lys Asp

465 470 475 480

Arg Met Ser Met Ala Val Gly Leu Glu Val Arg Val Pro Phe Cys Asp

485 490 495

His Arg Leu Met Asp Tyr Val Phe Asn Val Pro Trp Ala Met Lys Ser

500 505 510

Phe Asp Gly Arg Glu Lys Ser Leu Leu Arg Ala Ala Val Arg Asp Val

515 520 525

Leu Pro Asn Ser Val Leu Glu Arg Thr Lys Thr Pro Phe Pro Ala Thr

530 535 540

Gln Asp Thr Arg Tyr Glu Gln Ala Leu Arg Ala Glu Leu Arg Glu Val

545 550 555 560

Leu Ala Asp Pro Asp Ala Pro Val Arg Pro Leu Leu Asn Thr Ala Arg

565 570 575

Val Asn Arg Val Leu Gly Arg Glu Leu Asp Asp Phe Ser Leu Pro His

580 585 590

Asp Arg Gly Gly Ile Glu Met Ala Leu Trp Leu Asn Arg Trp Leu Ala

595 600 605

Ser Tyr Glu Val Thr Val Thr Val

610 615

<210>20

<211>259

<212>PRT

<213>Streptomyces flaveolus

<400>1

Met Pro Asp Arg Ser Ser Ala Glu His Gln Leu Trp Leu Arg Gly Phe

1 5 10 15

His Gln Pro Lys Pro Gly Ala Pro Arg Leu Ile Cys Leu Pro His Ala

20 25 30

Gly Gly Ser Ala Ser Phe Tyr Phe Pro Val Ser Gln Ala Leu Ser Pro

35 40 45

Ala Val Asp Val Leu Ala Val Gln Tyr Pro Gly Arg Gln Asp Arg Arg

50 55 60

Gln Glu Ser Pro Ala Ala Ser Val Gln Glu Leu Ala Glu Gly Val Phe

65 70 75 80

Arg Ala Leu Asp Asp Gln Glu Asp Thr Pro Leu Ala Leu Phe Gly His

85 90 95

Ser Met Gly Ala Met Val Gly Phe Glu Leu Ala Arg Leu Leu Glu Ala

100 105 110

Ala Gly Arg Pro Pro Ala Val Leu Phe Ile Ser Gly Arg Arg Gly Pro

115 120 125

Ser Ile Val Trp Thr Glu Thr Val His Thr Met Asp Asp Glu Arg Leu

130 135 140

Ile Ala Glu Val Ala Lys Leu Glu Gly Thr Asp Ala Ala Leu Leu Gln

145 150 155 160

Asp Glu Glu Val Leu Arg Met Ile Leu Pro Ala Leu Arg Ala Asp Tyr

165 170 175

Arg Ala Val Glu Thr Tyr Gln Arg Thr Pro Gly Pro Arg Leu Ser Cys

180 185 190

Pro Phe Val Val Met Thr Gly Asp Ala Asp Pro Arg Val Thr Pro Asp

195 200 205

Glu Ala Arg Thr Trp Ala Glu Glu Thr Asp Gly Ala Phe Glu Leu Glu

210 215 220

Val Tyr Pro Gly Ala His Phe Tyr Leu Val Ala Gln Gln Gln Ala Val

225 230 235 240

Leu Ala Arg Ile Glu Ala Thr Met Arg Arg Leu Gly Ser Thr Thr Gly

245 250 255

Ala Val Val

<210>21

<211>453

<212>PRT

<213>Streptomyces flaveolus

<400>1

Val Asn Glu Ile Leu Ser Ala Val Leu Ser Ala Glu Ala Thr Ala Ala

1 5 10 15

Asp Phe Ala Ala Leu Pro Leu Pro Glu Ser Tyr Arg Ala Ile Thr Val

20 25 30

His Lys Asp Glu Thr Glu Met Phe Thr Gly Val Gly Pro Ala Asp Lys

35 40 45

Asp Pro Arg Lys Ser Leu His Leu Asp Glu Val Pro Val Pro Glu Leu

50 55 60

Gly Pro Gly Glu Ala Leu Val Ala Val Met Ala Ser Ser Val Asn Tyr

65 70 75 80

Asn Ser Val Trp Ser Ser Ile Phe Glu Pro Leu Pro Thr Phe Ala Phe

85 90 95

Leu Glu Arg Tyr Gly Arg Arg Gly Glu Leu Ala Lys Arg His Asp Leu

100 105 110

Pro Tyr His Val Ile Gly Ser Asp Leu Ala Gly Val Val Leu Arg Thr

115 120 125

Gly Pro Gly Val Gln Ala Trp Lys Pro Gly Asp Glu Val Val Ala His

130 135 140

Cys Leu Ser Val Glu Leu Glu Ser Ser Asp Gly His Asp Asp Thr Met

145 150 155 160

Met Asp Pro Glu Gln Arg Ile Trp Gly Phe Glu Thr Asn Phe Gly Gly

165 170 175

Leu Ala Glu Leu Ala Leu Val Lys Ser Asn Gln Leu Met Pro Lys Pro

180 185 190

Arg His Leu Thr Trp Glu Glu Ala Ala Ala Pro Gly Leu Val Asn Ser

195 200 205

Thr Ala Tyr Arg Gln Leu Val Ser Arg Asn Gly Ala Gln Met Lys Gln

210 215 220

Gly Asp Asn Val Leu Ile Trp Gly Ala Ser Gly Gly Leu Gly Ser Tyr

225 230 235 240

Ala Thr Gln Leu Ala Leu Ala Gly Gly Ala Asn Pro Val Cys Val Val

245 250 255

Ser Thr Pro Gln Lys Ala Ala Ile Cys Arg Ser Met Gly Ala Glu Ala

260 265 270

Val Ile Asp Arg Asp Ala Glu Asp Tyr Arg Phe Trp Arg Asp Gly Asn

275 280 285

Thr Gln Asp Pro Arg Glu Trp Lys Arg Phe Gly Lys Arg Ile Arg Glu

290 295 300

Leu Thr Gly Gly Glu Asp Val Asp Ile Val Phe Glu His Pro Gly Arg

305 310 315 320

Glu Thr Phe Gly Ala Ser Val Phe Val Ala Arg Lys Gly Gly Thr Ile

325 330 335

Val Thr Cys Ala Ser Thr Ser Gly Tyr Glu His Gln Tyr Asp Asn Arg

340 345 350

Tyr Leu Trp Met Ser Leu Lys Arg Ile Ile Gly Ser His Phe Ala Asn

355 360 365

Tyr Arg Glu Ala Trp Glu Ala Asn Arg Leu Val Ala Lys Gly Arg Ile

370 375 380

His Pro Thr Leu Ser Lys Val Tyr Pro Leu Glu Glu Thr Gly Gln Ala

385 390 395 400

Ala Phe Asp Val Tyr Gly Asn Val His His Gly Lys Val Gly Val Leu

405 410 415

Gly Leu Ala Pro Thr Glu Gly Leu Gly Val Arg Asp Glu Glu Met Arg

420 425 430

Ala Arg His Val Gly Ala Ile Asn Arg Phe Arg Pro Ser Ala Glu Pro

435 440 445

Gln Thr Glu Pro Val

450

<210>22

<211>71

<212>PRT

<213>Streptomyces flaveolus

<400>1

Met Ser Asn Pro Phe Asp Lys Glu Asp Gly Ser Phe Phe Val Val Val

1 5 10 15

Asn Met Glu Gly Gln His Ser Leu Trp Pro Ala Phe Ala Glu Ile Pro

20 25 30

Ala Gly Trp Ser Ile Val His Gly Glu Thr Asp Lys Ala Ala Cys Leu

35 40 45

Glu Tyr Val Ala Ala His Trp Thr Asp Met Arg Pro Arg Ser Leu Thr

50 55 60

Asp Ala Ala Pro Thr Pro Glu

65 70

<210>23

<211>178

<212>PRT

<213>Streptomyces flaveolus

<400>1

Met Pro Arg Arg Pro Gln Ser Arg Thr Pro Leu Arg Lys Gln Ser Val

1 5 10 15

Asp Asn Lys Val Phe Lys Cys Gln Gly Cys Arg Met Ser Ser Ile Pro

20 25 30

Pro Gln Pro Ala Asp Ala Phe Thr Ala Pro Ser Ala His Glu Ala Arg

35 40 45

Val Gln Arg Ala Tyr Thr Ser Leu Phe Arg Ile Ala Glu Arg His Ala

50 55 60

Ala Thr Asp Gly Gln Arg Arg Arg Gln Ala Gln Ser His Met Ile Ser

65 70 75 80

Pro Tyr Glu Ala Val Arg Leu Val Ser Phe Leu Leu Ser Gly Ala Ala

85 90 95

Gln Leu Glu Gly Glu Glu Pro Glu Val Asp Arg Ala Asp Ile Thr Ala

100 105 110

Ala Leu Ser Leu Leu Pro Leu Ala Arg Ala Glu Met Asp Glu Val Glu

115 120 125

Ala Gly Ile Leu Lys Met Ala His Gly Arg Gly Met Thr Trp Ser Glu

130 135 140

Ile Ala Phe Gly Leu Gly Leu Ala Thr Pro Gln Ala Ala Arg Gln Arg

145 150 155 160

His Glu Arg Leu Ala Ser Arg Met Glu Ser Gly Ala Glu Lys Lys Ala

165 170 175

Asp Gly

<210>24

<211>152

<212>PRT

<213>Streptomyces flaveolus

<400>1

Val Gln Ala Ser Ser Gly Glu Thr Thr Pro Thr Thr Ser Arg Pro Gly

1 5 10 15

Arg Thr Pro Asn Trp Pro Ala Phe Ala Arg Ala Ser Glu Ala Glu Thr

20 25 30

Arg Phe Ala Gly Asp Pro His Phe Pro Pro Ile Ala Asn Gly Leu Asp

35 40 45

Tyr Leu Val Ser Val Val Glu Leu Leu Gln Arg Glu Lys Gly Ala Gly

50 55 60

Ser Ala Arg Asp Leu Lys Tyr Ala Val Leu His Leu Ala Ala Gly Ala

65 70 75 80

Glu Val Leu Pro Lys Ala Arg Leu Leu Met Glu His Trp Ser Leu Val

85 90 95

Phe Thr Asp Pro Gly Ala Ala Thr Arg Thr Ala Leu Glu Asp Gly Ser

100 105 110

Leu Ser Ser Cys Thr Pro Glu Arg Thr Arg Lys Arg Leu Asn Arg Arg

115 120 125

Trp Thr Ser Ala Ser Pro Ala Arg Pro Ala Ser Met Thr Ser Arg Gly

130 135 140

Ala Thr Ala Ala Glu Pro Tyr Ile

145 150

<210>25

<211>144

<212>PRT

<213>Streptomyces flaveolus

<400>1

Val Ser Leu Ile Glu Val Gly Pro Gly Gln Val Glu Leu Val Val Arg

1 5 10 15

Gly Pro Gly Val Leu Ala Thr Ser Val Arg Leu Phe Asp Trp Ser Arg

20 25 30

Ala Asp Glu Tyr Glu Thr Val Phe Ala Val Glu Ala Ile Ala Asp Gly

35 40 45

Val Arg Ala Arg Leu Glu Asn Val Thr Val Thr Val Trp Asp Asp Met

50 55 60

Ser Ala Phe Phe Asp Gly Leu Ala Arg Asp Phe Arg Gly Trp Asp Gly

65 70 75 80

Glu Arg Val Trp Ile Asn Asn His Leu Val Val Thr Ala Thr Phe Gly

85 90 95

Ser Gly Gly His Val Tyr Val Asp Trp Thr Leu Gln Ser Gly Phe Phe

100 105 110

Pro Gly Asp Trp Lys Cys Thr Val Thr Thr Val Ile Glu Ala Gly Glu

115 120 125

Gly Met Thr Ala Val Ala Ala Asp Leu Arg Glu Phe Leu Arg Gln Glu

130 135 140

Claims (3)

1. SFA A biosynthesis related genes, it is characterized in that, described gene is precursor complex functionality gene sfaJ, the nucleotides sequence of described sfaJ gene is classified 96225-97388 the base of SEQ ID NO.:1 as, length is 1164 base pairs, coding zinc refers to desaturase, and length is 388 amino acid.

2. the coded albumen of SFA A biosynthesis related genes as claimed in claim 1, is characterized in that, described proteins encoded is the albumen of sfaJ coded by said gene, and its aminoacid sequence is as shown in SEQ IDNO.:13.

3. sfaJ gene knockout mutant strain, it is characterized in that, described mutant strain produces the faint yellow streptomycete of bacterium (Streptomyces flaveolus) derived from the SFA of wild-type, and the sfaJ gene is knocked, thereby do not produce SFA, wherein the nucleotides sequence of sfaJ gene is classified 96225-97388 the base of SEQ ID NO.:1 as.

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