CN101063140B - Vancocin biological synthesis gene cluster - Google Patents
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- CN101063140B CN101063140B CN2007100368615A CN200710036861A CN101063140B CN 101063140 B CN101063140 B CN 101063140B CN 2007100368615 A CN2007100368615 A CN 2007100368615A CN 200710036861 A CN200710036861 A CN 200710036861A CN 101063140 B CN101063140 B CN 101063140B
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Abstract
The invention discloses an oriental quasi-non-branch acid bacteria A. orientalis vancocin biological synthetic gene cluster, which is characterized by the following: comprising twenty six genes; encoding and catalyzing vancocin precursor synthase, vancocin synthase and vancocin modifying enzyme. This invention can be used to constitute biology and synthesize vancocin.
Description
Technical field
The invention belongs to technical field of bioengineering, specifically, relate to a kind of microbiotic biological synthesis gene cluster, more particularly, relate to a kind of vancocin biological synthesis gene cluster as glycopeptide antibiotics.
Background technology
Vancomycin (Vancomycin), its structure are that Micormick in 1956 etc. separate a kind of glycopeptide antibiotics that obtains from a strain east amycolatosis fermented liquid as shown in Figure 1, are representative a kind of in the glycopeptide antibiotics that produces of actinomycetes.
The eighties in 20th century, because a large amount of uses of β-Nei Xiananleikangshengsu, cause the infection that causes by methicillin resistance staphylococcus aureus (MRSA) popular gradually.In this case, vancomycin more and more causes people's attention, be the choice drug that is used for the treatment of the treating severe infectious diseases that causes by MRSA at present clinically, and be described as " last line of defense that the mankind tackle the intractable Resistant strain " and " trump microbiotic " by international microbiotic expert.
Along with being on the rise and the development of modern biotechnology and chemical synthesising technology of bacterial drug resistance, the biosynthetic pathway of glycopeptide antibiotics such as vancomycin there has been more deep understanding.Had been found that now the antibiotic biosynthesis gene of many vancomycin families with and corresponding albumen.Along with finishing of the biological synthesis gene cluster sequencing of Chloroemomycin, deepened the understanding of people, and can infer the function of each gene according to this sequence in the gene.Subsequently, the sequence of the biological synthesis gene cluster of the partial sequence of the biological synthesis gene cluster of Balhimycin, sugar based Compound C omplestatin and A47934 also obtains in succession.
According to the study, natural vancomycin biosynthesizing had for 35 steps.Five kinds of amino acid monomers freely wherein, under the catalysis of enzyme, generate 7 kinds of non-protein amino acids, this 7 seed amino acid is synthetic seven linear peptides on non-ribosomal peptide synthetase (NRPS) fabrication line, its aryl side chain combination, crosslinked in time under the catalysis of crosslinking enzyme then, form complicated vancomycin seven peptide backbones, last MDP-glucose and sugared through the ages ammonia are on seven peptide backbones that are connected to vancomycin under the effect of glycosyltransferase.Although whole chemosynthesis of vancomycin have all realized, owing in the vancomycin structure a lot of reactive groups are arranged, making needs at first to carry out protective reaction in the building-up process.Therefore, as will also needing to seek in addition a kind of production technology, to reduce cost as medicine.
1985, thus Hopwood etc. at first produce new heterozygosis compound by carry out transgenosis between the generation bacterium of different compounds, have from then on started a kind of new technology---group and biosynthesizing.Thereby the combination biosynthesizing is to obtain the product structure analogue or have the active novel derivative of potential source biomolecule by the gene in the natural product biosynthetic pathway is regulated.In addition, the research of biological synthesis gene clusters such as Chloroeremycin and Balhimycin is also laid a good foundation for utilizing the combination biosynthesizing to create new heterozygosis glycopeptide antibiotics.
At present new research and development mainly concentrate on research and transformation aspect to the glycosylation mode for heterozygosis glycopeptide antibiotics such as vancomycins, but most up to now research is the heterogenous expression that carries out in streptomycetes such as colon bacillus or S.toyocanesis.U.S. Christopher leader's group and German Wohlleben leader's group a lot of enzymes in the vancomycin biosynthetic pathway have been carried out vivoexpression, and to zymologic property, three-dimensional arrangements etc. have been carried out detailed research, for sufficient preparation has been done in the combination biosynthesizing of glycopeptide antibiotics such as vancomycin.Also do not have but make up biosynthetic research at present in glycopeptide antibiotics such as vancomycin produce thalline, its key just is to lack perfect amycolatosis genetic operating system.
Summary of the invention
Purpose of the present invention just is to provide a kind of vancocin biological synthesis gene cluster.
Vancocin biological synthesis gene cluster of the present invention comprises the upstream and downstream part regulating and controlling sequence of 26 genes and these genes altogether, and its nucleotide sequence is shown in SEQ ID NO:1.
26 genes of vancomycin synthetic gene of the present invention bunch are specially:
1, vancomycin precursor synthase gene: vcm1, vcm17, vcm18, vcm13, vcm23, vcm24, vcm25, vcm26, vcm22, vcm19, vcm21, vcm11, vcm20, vcm14, vcm15 and vcm16, totally 16;
Wherein: vcm1, vcm17, vcm18 and the vcm13 prephenate dehydrogenase (prephenatedehydrogenase) of encoding respectively, hydroxymandelic acid synthetic enzyme Hmas, hydroxymandelic acid oxydase Hmo and hydroxyphenylglycine transaminase HpgT, these 5 enzymes are responsible for the synthetic of catalysis hydroxyphenylglycine (HPG, the amino acid in a non-natural source in vancomycin seven peptide backbones);
Vcm23, vcm24, vcm25 and the vcm26 dihydroxyphenyl acetic acid synthetic enzyme of encoding respectively, alkene acyl CoA lytic enzyme, hydroxyacyl dehydrogenase and alkene acyl CoA lytic enzyme, these 4 enzymes are responsible for the synthetic of catalysis dihydroxy-benzene glycine (DHPG, the amino acid in another non-natural source in vancomycin seven peptide backbones);
Vcm22, vcm19, vcm21, vcm11 and the vcm20 epimerase of encoding respectively, NDP-hexose dehydrogenase, C3-transaminase, C3-methyltransgerase and keto reductase, these 5 enzymes are responsible for catalysis osamine synthetic through the ages;
Vcm14, vcm15 and the vcm16 lytic enzyme of encoding respectively, peptide synthetase VCP4, P450 oxydase, these 3 enzymes are responsible for the synthetic of catalysis beta-hydroxy tyrosine.
2, vancomycin synthase gene: vcm2, vcm3, vcm4, vcm9 and vcm10, totally 5;
Wherein: vcm2, vcm3 and the vcm4 non-ribosomal peptide synthetase of encoding respectively, i.e. Vcp1, Vcp2 and Vcp3, these 3 non-ribosomal peptide synthetases include 7 modules altogether, and 24 structural domains are responsible for the biosynthesizing of catalysis vancomycin seven peptide backbones;
Vcm9 and vcm10 be encoding glycosyl transferring enzyme GtfE and GtfD respectively, these 2 enzymes be responsible on the 4th amino acids that catalysis D-glucose is connected to vancomycin seven peptide backbones respectively and through the ages osamine be connected on the D-glucose;
3, vancomycin modification gene: vcm12, vcm8, vcm11, vcm5, vcm6 and vcm7, totally 6;
Wherein: vcm12 coding N-methyltransgerase, this enzyme are responsible for the catalysis Methyl transporters to the N end nitrogen-atoms of first amino acid (leucine) of vancomycin seven peptide backbones;
The vcm8 chlB4 of encoding, this enzyme is responsible for the chlorination of the 2nd of catalysis vancomycin seven peptide backbone and the 6th amino acids;
Vcm11 coding C-methyltransgerase, this enzyme are responsible for the catalysis methylation reaction of the carbon atom that links to each other with nitrogen-atoms of osamine (this enzyme also participate in simultaneously catalysis synthesizing of osamine) through the ages through the ages;
Vcm5, vcm6 and the vcm7 oxydase Oxy1 that encodes respectively, Oxy2 and Oxy3, these 3 enzymes are responsible for oxidation cross-linked between catalysis vancomycin seven peptide backbones.
The nucleotide sequence of gene in the gene cluster of the present invention can east amycolatosis genome be a template, adopts the PCR method amplification in vitro to obtain.
Gene cluster nucleotide sequence of the present invention can carry out Error-prone PCR or DNA rearrangement (DNA shuffling) etc. by the different piece of sequence or the homologous sequence in other sources, thereby the acquisition activity is higher and/or the target protein of other performance changes.
Gene cluster nucleotide sequence of the present invention, the corresponding gene that can be used to interrupt the east amycolatosis is expressed, and comprises variously interrupting corresponding expression of gene by the mode of changing with single cross or the mode of double exchange.The east amycolatosis mutant strain that lacks one or more genes can be used for producing the different derivatives of vancomycin, also can be used to simultaneously to further investigate the route of synthesis of natural product and/or pathways metabolism etc.
Gene cluster nucleotide sequence of the present invention, can be used to the corresponding gene of east amycolatosis that doubles, but comprise gene clone in the carrier of self-replicating, express in the amycolatosis in the Orient, or be cloned in the integrative vector, be incorporated into the genome specific site and express.The pathways metabolism that can study the east amycolatosis is expressed in crossing of specific gene, also can cause the accumulation of some natural product.
Gene cluster nucleotide sequence of the present invention, the open reading frame that not only comprises the coded protein that participates in vancomycin biosynthesizing bunch, but also comprise the non-coding nucleotide sequence of gene upstream and downstream, the regulating and controlling sequence that wherein comprises genetic expression, regulating and controlling sequence provides condition for the regulation and control of the genetic expression of research east amycolatosis.
Gene cluster nucleotide sequence of the present invention, can be used for locating the Cowes plasmid in the library of more east amycolatosis, these Cowes plasmids include the nucleotide sequence among the present invention, the upstream of the east amycolatosis vancomycin composite variety that simultaneously also contains the present invention and covered or the sequence in downstream.
Gene cluster nucleotide sequence of the present invention can be used for being cloned in the different expression vectors, obtains recombinant expressedly in foreign host, and expressed proteins can be used for the property research and/or the vitro enzyme repercussion study of enzyme.
Description of drawings
Fig. 1 vancomycin structural formula.
Fig. 2 vancomycin biosynthetic pathway.
DPG building-up process in Fig. 3 vancomycin biosynthetic pathway.
DHPG building-up process in Fig. 4 vancomycin biosynthetic pathway.
Glycosylation process in Fig. 5 vancomycin biosynthetic pathway.
Osamine building-up process through the ages in Fig. 6 vancomycin biosynthetic pathway.
Oxidation cross-linked process in Fig. 7 vancomycin biosynthetic pathway.
The mode chart of Fig. 8 vancocin biological synthesis gene cluster.
The positive cosmid plasmid electrophorogram of Fig. 9 pcr amplification.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.Should be understood that following examples only are used to the present invention is described but not are used to limit scope of the present invention.
The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, the condition described in " molecular cloning: laboratory manual " (New York:Cold Spring Harbor Laboratory Press, 1989) is carried out.
The present invention will come from the genome of east amycolatosis A.orientalis, partially digested through Sau3A I, obtain the endonuclease bamhi of main band in the 30-45kb scope, and described endonuclease bamhi is carried out terminal dephosphorization treatment with alkaline phosphatase (CIAP); Carrier-pellet disconnection with the Supercosl that passes through BamH I and Nhe I double digestion connects again, uses Stratagene Gigapack III XL Packaging Extract package kit, makes up and also obtains east amycolatosis genomic library.
With east amycolatosis GtfE Gene Partial sequence fragment is that template prepares probe, with library hybridization, therefrom obtains to contain the positive Cowes plasmid (cosmid) with the probe homologous sequence.Choose wherein 1 relatively large positive cosmid plasmid, carry out nucleotide sequencing, and after sequencing result is analyzed, obtained the nucleotide sequence of vancocin biological synthesis gene cluster.
Embodiment 1: the foundation of east amycolatosis genomic library
Intend not having branch bacillus gene group with reference to the method extracting east of " molecular cloning: laboratory manual ", intend not having branch acid gene group with the partially digested east of Sau3A I enzyme then, select main band to insert fragment at the east of 30～50kb amycolatosis genome.
With Supercosl (Stratagene company) carrier, be cut into linear segment with BamH I enzyme, with the T4 ligase enzyme it is connected 24 hours with above-mentioned partially digested east amycolatosis genomic fragment at 16 ℃ then, obtain to connect product.
Adopt Stratagene Gigapack III XL Packaging Extract test kit,, the connection product that obtains is packaged into phagemid, and is transformed in the intestinal bacteria, be built into east amycolatosis genomic library according to the operational manual of test kit.
The screening by hybridization that contains the cosmid of specific gene (GtfE) in embodiment 2, the library
With the GtfE gene as template, adopt DIG High Prime DNA Labeling and Detection StrarterKit I test kit (Roche company) test kit, preparation probe, screening and acquisition positive colony in the amycolatosis genomic library eastwardly.
The positive colony bacterium that obtains is cultivated, and the positive cosmid of extracting, with it as template, as specificity amplification primer, carry out pcr amplification with Ep1 (SEQ ID NO:28) and Ep2 (SEQ ID NO:29), to the amplification agarose electrophoresis, checking cosmid size, the result as shown in Figure 9.
According to the result of Fig. 9, choose No. 311 cosmid and check order, by Veceor NTI advance 10 (invitrogen company) sequencing result is analyzed.
The result of order-checking shows that the nucleotide sequence of vancocin biological synthesis gene cluster of the present invention comprises 26 genes altogether shown in SEQ ID NO:1, and these 26 genes can reduce 3 classifications, and are specific as follows:
1, vancomycin precursor synthase gene: vcm1, vcm17, vcm18, vcm13, vcm23, vcm24, vcm25, vcm26, vcm22, vcm19, vcm21, vcm11, vcm20, vcm14, vcm15 and vcm16, totally 16;
2, synthetic interior gene: the vcm2 of vancomycin, vcm3, vcm4, vcm9 and vcm10, totally 5;
3, vancomycin modification gene: vcm12, vcm8, vcm11, vcm5, vcm6 and vcm7, totally 6;
Wherein, catalysis vancomycin precursor synthetic gene can further be subdivided into 4 classifications, is specially:
1) HPG synthase gene: vcm1, vcm17, vcm18 and vcm13, totally 4;
These 4 genes prephenate dehydrogenase (prephenate dehydrogenase) of encoding respectively, hydroxymandelic acid synthetic enzyme Hmas, hydroxymandelic acid oxydase Hmo and hydroxyphenylglycine transaminase HpgT, these 4 enzymes are responsible for the synthetic of catalysis HPG;
Wherein, the nucleotide sequence of vcm1 is shown in SEQ ID NO:2; The nucleotide sequence of vcm17 is shown in SEQ ID NO:18; The vcm18 nucleotide sequence is shown in SEQ ID NO:19; The nucleotide sequence of vcm13 is shown in SEQ ID NO:14.The position of these 4 genes in gene cluster, corresponding sequence numbering and function are as shown in table 1.
Table 1, HPG synthase gene
| vcm | The position is (initial ... stop) | SEQ?ID?NO. | The enzyme of |
| 1 | 1…849 | 2 | Prephenate dehydrogenase |
| 17 | 47321…48355 | 18 | The hydroxymandelic acid synthetic enzyme |
| 18 | 48352…49431 | 19 | The hydroxymandelic acid oxydase |
| 13 | 41900…43204 | 14 | The hydroxyphenylglycine transaminase |
2) DHPG synthase gene: vcm23, vcm24, vcm25 and vcm26, totally 4;
These 4 genes dihydroxy-benzene glycine synthetic enzyme of encoding respectively, alkene acyl CoA lytic enzyme, hydroxyacyl dehydrogenase, and alkene acyl CoA lytic enzyme, these 4 enzymes are responsible for the synthetic of catalysis DHPG;
Wherein, the nucleotide sequence of vcm23 is shown in SEQ ID NO:24; The nucleotide sequence of vcm24 is shown in SEQ ID NO:25; The nucleotide sequence of vcm25 is shown in SEQ ID NO:26; The nucleotide sequence of vcm26 is shown in SEQ ID NO:27.The position of these 4 genes in gene cluster, corresponding sequence numbering and function are as shown in table 2.
Table 2, DHPG synthase gene
| vcm | The position is (initial ... stop) | SEQ?ID?NO. | The enzyme of coding |
| 23 | 54523…56346 | 24 | The dihydroxyphenyl acetic acid synthetic enzyme |
| 24 | 56373…57002 | 25 | Alkene acyl CoA |
| 25 | 56999…58225 | 26 | Hydroxyacyl dehydrogenase |
| 26 | 58222…59013 | 27 | Alkene acyl CoA lytic enzyme |
3) osamine synthase gene: vcm22 through the ages, vcm19, vcm21, vcm11 and vcm20, totally 5;
These 5 genes epimerase of encoding respectively, NDP-hexose dehydrogenase, C-3 methyltransgerase, C-3 transaminase, and keto reductase, these 5 enzymes are responsible for catalysis osamine synthetic through the ages.
Wherein, the nucleotide sequence of vcm22 is shown in SEQ ID NO:23; The nucleotide sequence of vcm19 is shown in SEQ ID NO:20; The nucleotide sequence of vcm21 is shown in SEQ ID NO:24; The nucleotide sequence of vcm11 is shown in SEQ ID NO:12; The nucleotide sequence of vcm20 is shown in SEQ ID NO:21.The position of these 4 genes in gene cluster, corresponding sequence numbering and function are as shown in table 3.
Table 3, osamine synthase gene through the ages
| vcm | The position is (initial ... stop) | SEQ?ID?NO. | The enzyme of coding |
| 22 | 54523…55140 | 22 | Epimerase |
| 19 | 51015…52421 | 20 | The NDP-hexose dehydrogenase |
| 21 | 53390…54499 | 22 | The C3 transaminase |
| 11 | 38984…40210 | 12 | The C3-methyltransgerase |
| 20 | 52422…53393 | 21 | Keto reductase |
4) beta-hydroxy tyrosine synthase gene: vcm14, vcm15 and vcm16, totally 3;
These 3 genes lytic enzyme of encoding respectively, peptide synthetase VCP4 and P450 oxydase, these 3 enzymes are responsible for the synthetic of catalysis beta-hydroxy tyrosine oxidase.
Wherein, the nucleotide sequence of vcm14 is shown in SEQ ID NO:15; The nucleotide sequence of vcm15 is shown in SEQ ID NO:16; The nucleotide sequence of vcm16 is shown in SEQ ID NO:17.The position of these 3 genes in gene cluster, corresponding sequence numbering and function are as shown in table 4.
Table 4, beta-hydroxy tyrosine synthase gene
| vcm | The position is (initial ... stop) | SEQ?ID?NO. | The enzyme of coding |
| 14 | 43435…44265 | 16 | Lytic enzyme |
| 15 | 44316…45998 | 17 | Peptide synthetase VCP4 |
| 16 | 46012…47202 | 18 | The P450 oxydase |
The vancomycin synthase gene can further be subdivided into 2 classifications, is specially:
1) non-ribosomal peptides synthase gene: vcm2, vcm3 and vcm4, totally 3;
These 3 genes non-ribosomal peptide synthetase Vcp1 that encodes respectively, Vcp2 and Vcp3, these 3 enzymes are responsible for the biosynthesizing of catalysis vancomycin seven peptide backbones;
Wherein, the nucleotide sequence of vcm2 is shown in SEQ ID NO:3; The nucleotide sequence of vcm3 is shown in SEQ ID NO:4; The nucleotide sequence of vcm4 is shown in SEQ ID NO:5.The position of these 3 genes in gene cluster, corresponding sequence numbering and function are as shown in table 5.
The gene of table 5, non-ribosomal peptide synthetase
| vcm | The position is (initial ... stop) | SEQ?ID?NO. | The enzyme of |
| 2 | 2947…12405 | 3 | |
| 3 | 12758…24910 | 4 | |
| 4 | 24931…30507 | 5 | Vcp3 |
Described coding non-ribosomal peptide synthetase Vcp1,3 genes of Vcp2 and Vcp3 comprise 7 modules, 25 structural domains, described 7 modules are distributed in Vcp1 with 3/3/1 form, Vcp2, on these three subunits of Vcp3, each module has different selections and activation to corresponding amino acid, and the putting in order of described these modules determined amino acid whose the putting in order on vancomycin seven peptide backbones.Situation about above-mentioned subunit, module and structural domain sees table 6 for details.
Table 6, non-ribosomal peptides synthetic enzyme subunit, module and structural domain
Wherein: structural domain A is adenosine structural domain (Adenylation domain); Domain C is condensation structural domain (condensation domain); Structural domain T is sulfhydrylation structural domain (thiolation domain); Structural domain E is epimerization structural domain (epimerase domain).
Referring to the peptide synthetase modular structure among Fig. 3, include 3 modules on the Vcp1 subunit, i.e. module 1, module 2 and module 3.Wherein, module 1 includes 2 structural domains, is respectively structural domain A and structural domain T; Module 2 includes 4 structural domains, is respectively domain C, structural domain A, structural domain T and structural domain E; Module 3 includes 3 structural domains, is respectively domain C, structural domain A and structural domain T.
Include 3 modules on the Vcp2 subunit, i.e. module 4, module 5 and module 6, wherein, module 4 includes 4 structural domains, is respectively domain C, structural domain A, structural domain T and structural domain E; Module 5 includes 4 structural domains, is respectively domain C, structural domain A, structural domain T and structural domain E; Module 6 includes 3 structural domains, is respectively domain C, structural domain A and structural domain T.
Include 1 module on the Vcp3 subunit, promptly module 7, and this module includes 5 structural domains, are respectively domain C, structural domain A, structural domain T, domain C and structural domain TE.
In these modules, except that 1 of module contained structural domain A and structural domain T, remaining 6 module all contained structural domain A, these three structural domains of domain C and structural domain T, and the function of these three structural domains is respectively: the A territory is selected and activated amino acid; The catalysis of T territory forms aminoacyl-S-enzyme complex; The formation of C territory catalysis peptide bond, and guide the extension of peptide chain and the transposition that peptide chain is held to C by the N end.In these external the 2nd, 4,5 modules a structural domain E is arranged respectively also, the L type amino acid in the catalysis biological route of synthesis changes D type amino acid into, makes the amino acid of vancomycin seven peptide backbones of formation be the D-D-L-L-D-D-L form.
2) glycosyltransferase gene: vcm9 and vcm10, totally 2;
These 2 genes are encoded respectively osamine are through the ages transferred to required on vancomycin seven peptide backbones 2 glycosyltransferase GtfE and GtfD, wherein:
Vcm9 encoding glycosyl transferring enzyme GtfE, this enzyme are responsible for above the 4th amino acids that catalysis D-glucose is connected to vancomycin seven peptide backbones;
Vcm10 encoding glycosyl transferring enzyme GtfD, this enzyme be responsible for catalysis through the ages osamine be connected on the D-glucose.
Wherein, the nucleotide sequence of vcm9 is shown in SEQ ID NO:10; The nucleotide sequence of vcm10 is shown in SEQ ID NO:11.The position of these 2 genes in gene cluster, corresponding sequence numbering and function are as shown in table 7.
Table 7, glycosyltransferase gene
| vcm | The position is (initial ... stop) | SEQ?ID?NO. | The enzyme of coding |
| 9 | 36453…37679 | 10 | Glycosyltransferase GtfD |
| 10 | 37732…38958 | 11 | Glycosyltransferase GtfE |
The gene of the enzyme that the catalysis vancomycin is modified can further be subdivided into 4 classifications, is specially:
1) N-methyl transferase gene: vcm12, the enzyme of its coding, promptly the N-methyltransgerase is responsible for the catalysis Methyl transporters to the leucic N end of first amino acid of vancomycin seven peptide backbones nitrogen-atoms;
2) chlB4 gene: vcm8, the enzyme of its coding, promptly chlB4 is responsible for the chlorination of the 2nd of catalysis vancomycin seven peptide backbone and the 6th amino acids;
3) C-methyl transferase gene: vcm11, the enzyme of its coding, i.e. C-methyltransgerase, the methylation reaction of the carbon atom that responsible catalysis links to each other with nitrogen-atoms on the osamine through the ages; With
4) oxidase gene: vcm5, vcm6 and vcm7, totally 3, three enzymes of these 3 coded by said gene are respectively oxydase Oxy2, and Oxy1 and Oxy3 are responsible for oxidation cross-linked between catalysis vancomycin seven peptide backbones.Its oxidation cross-linked sequence of operation is followed successively by Oxy2 → Oxy1 → Oxy3.
Wherein, the nucleotide sequence of vcm12 is shown in SEQ ID NO:13; The nucleotide sequence of vcm8 is shown in SEQ ID NO:9; The nucleotide sequence of vcm11 is shown in SEQ ID NO:10; The nucleotide sequence of vcm5 is shown in SEQ ID NO:6; The nucleotide sequence of vcm6 is shown in SEQ ID NO:7; The nucleotide sequence of vcm7 is shown in SEQ ID NO:8.The position of these 6 genes in gene cluster, corresponding sequence numbering and function are as shown in table 8.
The gene of the enzyme that table 8, catalysis vancomycin are modified
| vcm | The position is (initial ... stop) | SEQ?ID?NO. | The enzyme of coding |
| 12 | 41077…41919 | 13 | The N-methyltransgerase |
| 8 | 34922…36397 | 9 | ChlB4 |
| 11 | 38984…40210 | 12 | The C3-methyltransgerase |
| 5 | 30801…31976 | 6 | Oxv1 |
| 6 | 32307…33530 | 7 | Oxy2 |
| 7 | 33657…34877 | 8 | Oxv3 |
Sequence table
＜110〉Shanghai Inst. of Life Science, CAS
＜120〉vancocin biological synthesis gene cluster
<130>061586N
<160>29
<170>PatentIn?version?3.1
<210>1
<211>59032
<212>DNA
＜213〉east amycolatosis
<400>1
gtgacggtga?ccatcgagaa?ggcgcttgtc?gtcggtacgg?gactgatcgg?cacctccgtc 60
gcgctggccc?ttcgcgaacg?gggcgtggcg?gtgtatctgt?ccgatgtaga?cgctcgggcc 120
gtccggctgg?cgggggacct?cggcgccggc?cgggaatgga?ccggtgagga?agccgacctg 180
gcgctgatcg?ccgtcccgcc?gcatctcgtg?ggtgagcgac?tggcggacct?gcagcggcga 240
ggagccgcgc?gggtgtacac?cgatgtggcg?agcgtcaagg?tcgacccgat?cgccgacgcg 300
gaacggctgg?gctgcgacct?gagttcctat?gtgccgggcc?atccgctcgc?cggccgggaa 360
cggtccggcc?cgtccgccgc?ccgctccgat?ctgttcctcg?gccgtccgtg?ggcgatctgc 420
cccggcgccg?agacggccgc?ggatgccgtc?cggctgacgc?gggaactggt?ttcgtcgtgc 480
ggagcgaggg?cggtcacggt?ggaggcggcc?gagcacgatg?cggcggtggc?gctggtgtcg 540
cacgctccgc?atgtggtggc?gtcggcgatg?gcggcgagtc?tcgcgggcgg?cgacgacgtc 600
gcgctgggcc?tgtcggggca?gggactccgc?gatgtgacgc?ggatcgcggc?cggggatccc 660
ttgctgtggc?gaaggattct?cgccgggaac?gcccagccgg?tcgccggggt?gctcgaccgg 720
atcgcgggcg?acctcgcggc?ggcggcctcg?gcgttgcggt?ccggcgatct?ggacgaggtg 780
acggatctgc?tgcggcgcgg?ggtggccggc?catggccgga?tccccggccc?gcgcggcgcc 840
aaagcaggct?gacccgccac?ggaacactcg?cgctccgacc?ccgcgcgaac?gtgtccaagc 900
gcggaaagtg?ctcgcttgga?ctcactcgtg?gacatcgact?cgattcagca?cgattgagat 960
cgccgacttg?gctcgtgaga?gaggtgaccg?tatggacgtg?gttctgcgct?tcgagggggt 1020
ggacaagagt?gagcacgatc?cggatccctg?ggtgacgaag?gtccgcaagg?ggaccgtgcg 1080
ccgggtgctc?gcctacttcc?gcccgcacgt?cggcaaggtg?gcgctcttcg?tgctcgtcgc 1140
cgtcctggag?gcgctgatcg?tcgtcgcgac?tccgttgctg?ctgaaggaac?tcatcgacaa 1200
cggcatcgtc?aagaacgacc?tcggggtcgt?gatctggatg?gccgtcctca?ccgccgggct 1260
cgccttcctg?ggcgcggcgc?tggcgctgct?gtcgggctac?atctccggga?agatcgggga 1320
ggggatcacc?tacgacctcc?gggtccaggc?gctcgaccat?gtccggcggc?tgccgatcgc 1380
gttcttcacc?cgcacgcaga?ccggggtgct?ggtcggacgg?ctgcacacgg?aactgatcat 1440
ggcgcagcag?catttcaccg?gtctgctcat?ggcggccacc?agcctggtca?tggtcgtggt 1500
ggtgctggcc?gagctgttct?atctttcgtg?gctcgtcgcc?gcgatctcgc?tgctgatcat 1560
cccgatcttc?ctcgtgccct?ggatccgggt?cgggcgggcg?atccagcggc?gcagcatccg 1620
gcagatggac?gagaacgccg?gtctcggcgg?gctgctccag?gagcggttca?acgtgcaggg 1680
ggcgatgctc?tccaaactct?tcggccggcc?ggacgaggag?atggccgagt?acaccgaacg 1740
tgccgggaag?atccgcgaag?tcggtgtgag?ccttgccgtc?tggggccgga?tggcgttcgt 1800
catgatggcg?ctgatggcct?cgctggccac?ggcgctcgtc?tacgggatcg?gtggcgggct 1860
cgtgctcgcg?ggggcgttcc?agctcggcac?gctggtcgcc?atcgccacgc?tgctcgggcg 1920
gatgttcggg?ccgatcaccc?agctgtccgg?gatgcaggag?ctggcgcaga?ccgtcgtggt 1980
gagtttttcc?cgggtcttcg?agctgctcga?cctcaagccg?ctcatccagg?aacgccccgg 2040
cgcggtcgcg?ctggagaagg?acgtggcgcc?ggacatcgag?ttcgagaacg?tgaagttccg 2100
ctatcccacc?gcggacgagg?tctcgctggc?gtcgctggag?catctgcgga?ccgagcggga 2160
gcgcggggaa?gtgtcgccgg?acgtcttgcg?cgacgtgagt?ttccacgtgg?gggccggcac 2220
cctcaccgcg?ctcgtcggcc?cgtcgggggc?ggggaagagc?accatcaccc?acctggtgtc 2280
ccggctgtac?gacccgaacg?ccgggaccgt?gcgcgtcggc?gggcacgacc?tgcgggacct 2340
cagcttcgca?tcgctgcgcg?aaacggtggg?tgtggtcagc?caggacgcct?atctcttcca 2400
caacacgatc?cgggagaacc?ttctctacgc?tcgtccgacc?gccaccgagg?aagagctgat 2460
cgaggcctgc?aagggggccc?agatctggga?tctgatcgaa?tccctgcccc?tcgggttgga 2520
caccgtctcg?ggtgaccgcg?gctaccgcat?gtcgggcggg?gagaagcaac?gactggccat 2580
cgccaggctg?ctgctgaagg?cgccgacgat?cgtcgtactc?gacgaagcca?ccgcgcacct 2640
ggactccgag?tcggaggcgg?ccgtccagcg?ggcgctcaag?accgccctgc?ggaaccggac 2700
gtcgctggtg?atcgcccacc?ggttgtccac?gatccgcgag?gccgaccaga?tcctcgtgat 2760
cgacggtggc?ggggtgcggg?agcgcgggac?gcacgacgag?ctgctggccc?aaggcggcct 2820
gtacgcggag?ctgtactaca?cgcagttcgc?cgacccggcg?aacgacgcgg?ccaagccgga 2880
agtcgaggaa?gccgagcttg?acgagcccga?gcctcagccg?gcgatccaac?ccatcggctt 2940
cggaggatga?cgatgaattc?cgcagcgcgg?accacacgga?cggtgctgga?gctgttcgcg 3000
tcgcacgtgg?accggacgcc?cggcgcggtg?gccgtggtcg?acggtgaccg?ggtgctgaca 3060
taccggcagc?tcgacgagca?agcgagccgg?ttggccggat?gcctggcagg?ccggggaatc 3120
cgccgtggcg?accgggtcgc?ggtgatcatg?ggccggtcgg?cggacctggt?ggcggcgctg 3180
ctcggcgtgt?ggaaggcggg?ggcggcgtat?gtgccggtcg?acgccgccta?tcccgctccg 3240
cgcgtggaat?tcatggtgtc?ggactcggcg?gccgcgctga?cggtgtgctc?ggccgccacc 3300
cgttccgccg?taccggcggg?ggtggcgtcg?ctcgaagcgg?acgccgtcac?ggacgaggac 3360
gcgggcggca?ctccggtcgc?cgcggcgcgc?ccgggggatc?cggcgt?tcgt?gatgtacacg 3420
tccggctcta?cgggcacgcc?gaagggagtg?gttatctccc?agagccgtgt?cgcggagctg 3480
gtgcaggacc?ccggttggga?gatggggccg?gacgacgcgg?tgctcatgca?ctcgccgcac 3540
gccttcgacg?cgtccctgct?cgaaatctgg?acgccgctgt?cctcgggggc?ccgggtggtg 3600
atcgccgaac?cgggcgtgat?ggacgcccgg?cgcctgcggg?aagtggccgc?cgcgggagtg 3660
acgcgcgcgt?acctgaccgc?ggggagtttc?cgtgcggtgg?ctgaggaatc?gccggaatcc 3720
ttcgccgggt?tccgcgaggt?gctgaccggc?ggcgacgtgg?tgcccgtgca?cgcggtggag 3780
cgcgtgcggc?aggcctgccc?ccgggcgcgg?gtccggcata?tgtacggacc?gaccgaagcg 3840
acgatgtgcg?cgacgtggca?tctgctggag?ccgggtgacg?tgctggggac?ggtgctgccg 3900
atcgggcgtc?cgctggcggg?ccgcgcggtc?cacgtgctcg?acgagtcgct?gcggcccgtc 3960
gggccgggtg?tggtcggcga?cctgtatctc?tcgggaagcc?tggcggaggg?ctacttcggc 4020
aaggccgggc?tgacggcgga?acggttcgtg?gcggatccct?ccacgccggg?acagcggatg 4080
tactggacgg?gcgacctcgc?ccagtggacc?gccgagggcg?agctgctgtt?cgcgggccgg 4140
gccgacaacc?aggtgaaggt?ccgcgggttc?cggatcgaac?ccggcgagat?cgaggccgcg 4200
ctcatcgccc?agccggacgt?ccaggacgcc?gtggtggcgg?cgatcgacgg?acggctcatc 4260
ggttatgtgg?tggcgggtga?ggacgtcgac?cccgcgctcg?tccgcgagcg?tctccgtgcg 4320
gtgctgccgg?agtacctggt?cccggtcgcc?gtgctcgtgc?tggacgcgtt?gccgctgacc 4380
ggcaacggca?aggtggaccg?ggcggcgctg?cccgcacccg?agttcacggc?gagcgccacg 4440
agccgggagc?ccgccaccga?ggccgagcgt?gtcctttgtg?gactgttcgc?cgaggtgctc 4500
cggatcgacc?gggccggtgt?cgacgacgga?ttcttcgaac?tgggcgggga?ttcgatcctc 4560
gcgatgcgcc?tggcggcgcg?cgcggccaag?gccggtctgc?tgctgacgcc?ggtccagatc 4620
ttcgaggaga?ccactcccgc?gcggctggcc?gccgtggcgc?gggacctgcc?cgccgaccgg 4680
cccgccgacg?gtgccctgct?cagcctgacc?gaggcggaag?aggcggagct?ggcgctcgcc 4740
gctcccggcg?ccgacgagat?ctggccgctg?gccccgctgc?aggaggggct?gctcttcgaa 4800
tcgatcctcg?acgaccaggg?ccccgacatc?taccaggtgc?aggtgatcct?ggagctgacc 4860
ggcgaggtgg?acgagacccg?gctgcgagcc?gcgtgggaca?ccgtcgtccg?gcggcacgac 4920
gagctgcggc?tgaccttcca?ccggctcgaa?tcgggcaaga?ccgtgcaggc?cgtccacggg 4980
gatgtcaccg?tgccgtggcg?gacggtggat?ctgaccggcg?ccgacgacgt?cgacaccgcc 5040
ttcgaagccc?tggcgatgca?ggagcaggcg?catcggttcg?acctcgccac?ggcgccgctg 5100
atccggctgg?tgctcgtccg?ggtcggggag?gaccggcacc?ggctgctggt?cgtcatccac 5160
cacatcctgg?tcgacggctg?gtcggtggcg?gtcatcctca?acgaggcgtt?cgaggcctac 5220
gagcccggcg?ggcggctgcc?ggaacggcgg?gagggcgccg?gctatctcga?ctacctggct 5280
tggctggaga?ggcaggacaa?ggacgcggca?cgcgaggcct?ggcgggcgga?gctgtccgac 5340
ctcgacgagc?ccgcgctcat?cgcgtcggcg?tcggccgcga?cggactacga?ctaccgcgtc 5400
acccacctgc?cgcccgcgct?gcacgacggc?ctcctggaat?tcacccgtga?gcacgggctg 5460
acaccgagca?cggtggtgca?cgccgcctgg?gcgatggtgc?tgtcgcggct?cacgcggcgg 5520
accgacgtgg?tgttcggcac?catggtcgcg?acccgccccg?cggaactgcc?gggcatcgag 5580
tcgatgccgg?gcctgctgat?gagcgcgatg?ccggtccgtg?tgacgctgga?cggcgggcag 5640
gcggtcatgg?acatgctcac?cgggctgtac?ggccgtcagc tggccctcat?gccgcatcag 5700
cacctgggac?tgccggagat?ccagaaggcg?gccggacccg?gcgcgacctt?cgacacgctg 5760
ctggtggtcg?agaactaccc?gcgggaatac?gccggccggt?acaagtacct?gcgcacgatc 5820
gaagggaccc?actatccgct?gacgctcggc?atcaccccgg?gggaccggtt?caagatccag 5880
ctcggctact?ggcagggcca?ggtcccggac?gccgtcgccg?actcgctgct?ggagtggttc 5940
gtcggtgcca?tcggcgccct?ggtcgccgat?cccgccggtc?cggtggggcg?gatcggggtg 6000
ggcacggcgg?aggcacgcga?ctggcgaacg?ccactcgtgg?cgggggaacc?gctgcacacg 6060
ctggtggggc?ggatggccgc?cgagcggccg?gacggcgtgg?cgctggtgga?cggcgacggc 6120
gagctgacct?acgcgcggtt?gtgggatctc?tcggtgcggt?tcgccgccgt?gctgcgcgcc 6180
cacggcgtcg?gggcggagtc?ccgggtcggc?ctggtggtgg?gacggtcgtc?ctggtggacc 6240
gtcggcatgc?tgggcgtcct?gctcgcgggc?ggcacgttcg?tgccggtgga?cccggcgtat 6300
ccggacgagc?gcaaggaaac?gatcttccgc?agcgcggatc?cgatgctggt?ggtgtgcgcg 6360
gcgtcgaccc?gggggaccgt?gcccgccgag?tacgcggacc?ggtcgctggt?gatcgacgag 6420
gtcgagctga?cggcggcgac?ggaggaagag?ctgccgcggg?tggatccgcg?tagtgccgcg 6480
tacgtgatct?acacgtcggg?atcgacgggg?accccgaagg?gcgtcgtcgt?cacccacacc 6540
gggctgggga?acctggcgct?ggcgcatatt?gaacggttcg?ccgtgaagcc?gtcgtcgcgg 6600
gttttgcagt?tcgcggcgct?cggcttcgac?accatcgtgt?ccgaggtgat?gatggcgctg 6660
ctgtcgggag?ccacgctggt?ggtgccgccg?gagcgggagc?tgccgccgcg?gatggcgatg 6720
tccgaaacgc?tggcacggtg?ggacatcacg?caggtgaagg?cgccgccgtc?ggtgctgggc 6780
acggtcgacg?tgctgccgga?ccgggtggac?accgtggtcg?cggcgggcga?actctgcccg 6840
cccggcctgg?tggaccggat?gtccgcgaac?caccggatga?tcaacgccta?cgggccgacc 6900
gagaccacga?tctgcgcgac?gatgagcatg?ccgttgtcgc?cgggccagca?cccgatcccg 6960
ttcggcggac?cggttccggg?agtgcgcgcc?tatctgctgg?actcgttcct?gcgcccgttg 7020
ccgcccggca?tcaccggtga?gctgtatctg?gcgggcatcg?gggtggcgcg?cggttacctg 7080
ggccgtccgg?cgctgacggc?cgagcggttc?gtcgccgatc?cgttcgcccc?cggtgagcgg 7140
atgtaccgga?ccggggacct?ggcgtactgg?acggatcagg?gcgaactggt?gtccgccggg 7200
cgggccgatg?accaggtgaa?gatccgcggc?ttccgggtcg?aaccgcgcga?aatcgagttc 7260
gcgctgtccg?cgtacccggg?gatcacccag?gccaccgtga?ccgtccgcga?agaccgcctg 7320
atcgcctacg?tgacgccggg?agacatcgac?acccaggcgg?tgcgggcgca?gctcgcgtcg 7380
cggatgcccc?agtacatggt?gcccgcggcg?gtggtggcgc?tggacgtcct?gccgctgacg 7440
gcgcacggga?agatcgaccg?gcgcgcgttg?cccgaccccg?atttcgtcac?cgggacgccc 7500
gccagggagc?ccgccaccga?gaccgagcgg?gtgctgtgcg?agctgttcgc?cggcgtgctc 7560
gggctggagc?gggccggggt?ggacgacagc?ttcttcgacc?tcggcgggga?ctccatcctc 7620
tcgatgcagc?tcgcggcgcg?ggcgcggcgt?tcgggactga?gtttcaccgc?ggcggacgtc 7680
ttcgacggga?agaccccgga?gcgcatcgcg?cagctggcgg?aggtgtcttc?gccgccggaa 7740
caggccgctt?ccccgaagcc?cgacggtgtc?ggcgacgtcg?cctggacgcc?ggtgatgtcg 7800
ctgctgggcg?acggcgtcac?gggacccgga?ttcgcgcagt?ggatggtggt?cgcgacacct 7860
ccgggcctga?cggaaaaggc?tttggcggcg?ggattctcgg?ccgtggtggc?gacgcacgac 7920
atgctgcggg?cccgcgtcgt?cgaagacggg?cgcggacggc?ggctggtggt?cggcgagcgc 7980
ggggcggtgg?acccggccgg?gtggatcacc?cgcgtcgggc?tcgacggccg?ttcgccggac 8040
gaggccgtcg?aggacgcggc?acgcgaggcc?gtcggcaggc?tcgacccggc?ggcgggggtg 8100
atggcgcggg?tcgtgtggct?cgacgccgga?ccggaccggg?ccggccggtt?ggtggtggtc 8160
gtccatcacc?tgtcggtcga?cggtgtgtcc?tggcggatcc?tgctgtcgga?tctgcggtcc 8220
gcctgcgaag?cggcggccga?ggggcggacc?ccggcgctgg?aaccggtggg?cctgtcgttc 8280
aaacggtggg?cgagcctgct?gaaggagcag?gcggtctccg?cggaacgggt?cgcggaactg 8340
gaggcgtgga?aagcgatcct?cggaccggtg?gagcgcgggc?cgtccgagag?ccgggccgcg 8400
gggggcgaag?tgcgctcgcg?gtcgtgggtg?gtgccgaagg?cggagacacg?ggccttggtc 8460
ggccgggctc?cgctggtctt?ccactgcggg?gtgaacgacg?tcctgctcgc?ggggctggcc 8520
ggagcggtgg?cccggcggcg?cggtggcgac?gcggtgctgg?tggacgtgga?aggccacggc 8580
cgtcatccgg?ccgcgggcac?cgatctgtcc?cggacggtgg?gctggttcac?cagcgcgcat 8640
ccggtacggc?tggccgtggc?ggacaccgat?ctggcggacg?tgctcgccgg?tggcccggcg 8700
gccggtcggt?tgctgaagct?ggtcaaggag?cagtcgcggg?cggtgcccgg?cgacggcctc 8760
ggatacggca?tgttgcgcta?tctcaacgac?gtgacggggc?cggtactggc?ggcactgccg 8820
tcgccggaga?tcgggttcaa?ctacctcggc?aggttcgcca?ccgccgagaa?gggcgacgtg 8880
cgggcctggc?agccggtcgg?cgacctcggc?agtgcgcagg?aacccggaat?gggtctgccg 8940
cacgcgctcg?aagtcaacgc?gatcgtccag?gattcgccgg?acggtcccga?actgacgctc 9000
ctgctggaat?ggcaggaaga?cctgctggac?gaagccgagg?tcgaacgact?ggcccaggac 9060
tggctcgaca?tgctggccgg?gctggcccgg?caggcggacg?atcccgccgc?gggcgggcac 9120
accgcgtccg?acttcgagct?cgtcaccttg?gaccaggccg?agatcgaagc?cctcgaagcc 9180
gaatacgcgg?acgccggcgg?agtggccgac?gtgctcccgc?tttcgccgtt?gcagcacggg 9240
ctcgcgttcc?acgccgggta?cggcggtgac?ggggtcgacg?tctacaccgc?ccaggcggtg 9300
ctggacctga?gcggtccgct?ggacatgctg?ctgctgcgga?aatcggtgcg?cgggctgctg 9360
gaccggcacc?cgaacctgcg?cgccggattc?cggcacgccg?ccgccgggcc?cgcctatcag 9420
gtgatcccgg?gcgccgtgga?ggtgccggtg?tcctttgtgg?acgcgacggg?gtcggcggat 9480
ccggaggccg?aggcgacggc?ggtggccgag?gccgagcggg?ctcggccgtt?cgagctggcc 9540
aagcctccgc?tgctgcggat?cacggtggtg?gccctgagcc?gggacagcca?ccgcctggtg 9600
ctgaccaacc?atcacatcct?gctcgacggc?tggtcgacgc?cgctgctggt?ggacgaactg 9660
ctcacgctct?accgcgacgg?cgcggccccg?tccgcgctgg?cgccggtcac?cccgtaccgg 9720
gattacctgg?cctgggtcgg?cgaaaccgat?cgggacgcgt?cgatcaccgc?gtggcgcgag 9780
gcgctggcgg?gcctgccgga?accgaccctg?gtcgcggacg?accggccggt?gccggtccag 9840
gtgcccggcc?agatgtgggc?cgaactggac?gagacgttcg?ccgccgccct?gaccgcgcgg 9900
gcccgcgaat?gcggggtcac?ggtcagcacg?atgctgcaag?cggtgtgggg?cctggtgctg 9960
gcggcgacca?ccggacgcga?cgacgtggtg?ttcggggcgg?tggtgtccgg?gcgaccggcc 10020
gagctgcccg?ggatcgagac?catggtcggc?ctgttcatca?acaccgtgcc?ggtccgggtc 10080
cggacgcggc?cggagcagac?cttcgccgag?ctggtgcggc?agatccagga?cgagcagatc 10140
gcgttgctgg?cccatcacca?cgtcgggctc?gccgacatcc?agcaggccgc?cgggctgggc 10200
cggttgttcg?acaccctcgt?cgtctacgag?aactacccca?ggcccgacga?gaccgccgac 10260
acggagccgg?atcggttgcg?ggtgcgggga?atgaccgccg?ccgacgccac?ccactacccg 10320
ctggcgctgg?cggtcgtccc?cggcgacgtc?ctgcgcctgc?ggctggagca?ccagcccacg 10380
ctgttcacca?cggagcaggc?cgccgccgtg?ctggaccgct?tcacgcgggt?gctcgaagcc 10440
gtggtcgccg?agccgcggac?gccgctcccg?gcggtgccga?tcctgtccgc?cgccgaacag 10500
cgggacctgc?tggcgcgcaa?cgacaccgcg?gtggaggtgc?cggaccggac?gctcccggag 10560
tcgttcgccg?cgcaggccgc?cgccaccccg?gacgcgaccg?cggtggtctt?cgaggacctg 10620
tcgctgtcct?acgccgaact?cgacgcccgt?gccaatcagc?tggcgcgctg?gctcatcgaa 10680
cggggcgccg?ggcccgaagg?cctggtcgcg?gtgctgctgc?cccggtcgct?ggaactggtc 10740
gtcacggtgc?tggcgatcac?caagaccggt?gctgcctggc?tgccgatcga?tccggtctat 10800
ccggccgagc?gcgtgtcgta?catgctcgac?gacgcccggc?cgtcgctggt?gatcacctcc 10860
gcggcgctgt?cggcgtcgcc?cgccggtgag?gtggtggcgg?gcaggcaggc?gacggtggtg 10920
atcgacgatc?ccgtggccgt?tggcgagctg?gctgcccggg?atcgcgctcc?ggtcaccgac 10980
gcggaccgcg?tccggccgct?ggatccgcgt?catccggcgt?acctcatcta?cacctcgggc 11040
tccaccggca?ggcccaaggc?cgtcgtcgtc?acccaccgga?acctggcgaa?ctacctgttc 11100
cactgtggac?ggaagtaccc?ggggctgcgg?ggacgatcgc?tgatgcattc?gtcgatcgct 11160
ttcgatctga?cggtgaccgc?gctgctcact?ccgctgaccg?tgggcggaac?ggttcaggtc 11220
ggtgcgctgg?aagacctgat?cggcacggtg?gaaagcgccc?cggcgctgtt?cctcaaggcc 11280
acgccgagcc?atgtgaagac?gctggacacc?ggttcacggg?agatcgcggt?cgcgggcgac 11340
cttctgctcg?gtggcgagca?gttgcccgtc?gacacgatcg?cccactggcg?ccggacctac 11400
ccgaacaccg?tcgtggtcaa?cgaatacggc?ccgaccgagg?ccaccgtcgg?ctgcgtcgaa 11460
ttccggctcg?aacccgggca?gccggcgccg?tccggcgagg?tggtgccgat?cggcactccc 11520
atgacgaacg?cgcgggcgtt?cgtgctggat?tcgtggctgc?ggccggtgcc?gccgggcgcg 11580
gtgggcgaac?tgtacgtggc?gggcgcgggt?gtggcacgcg?gctatctcgg?gcggccgggg 11640
ctgaccgcga?cgcggttcgt?ggccgacccg?tccggggacg?gcgggctgat?gtaccggacc 11700
ggggacctgg?tgcggtggaa?cccggacggg?gaactgatgt?tcatcggtcg?cgtcgacgac 11760
caggtgaagg?tgcggggtta?ccggatcgag?cccggcgaga?tcgaagcggc?actggtggca 11820
caggagccgg?tgggacaggc?cgtggtgctg?gccaaggacg?gcgcctccgg?caaacggctg 11880
atcggttacg?tgaccgccgc?cggggagtcc?gaagtggacg?aagccgcggt?cctcgcgggc 11940
gtggccgccc?ggttgccgcg?gtacatggtg?ccggcggcgg?tggtggtgct?ggagacgctg 12000
ccgttgacgg?cgaacgggaa?agtggaccgg?gacgcgttgc?cggatcccga?tttcaacgga 12060
cgcgtcaccg?gacgggagcc?ggtcacggag?gccgagcggc?tgctgtgttc?gctcttcgcc 12120
gaggtgctcg?gactggagcg?ggcgggcgcg?gacgacagtt?tcttcgaact?gggtggcgat 12180
tcgatccttt?cgatgcaact?ggcggcgcgt?gcccaccggg?cgggaatagc?cttcggggcg 12240
cgtgaggtgt?tcgaacagcg?gacccccgcg?gcgatcgcgg?cggtcgtgga?acgggcggcg 12300
agcgctcccg?ccgcgagtga?acacgcgacg?tccgacgtcg?ctcttctcga?actggaccaa 12360
gacgagatcg?acgaattcga?agcggagttc?gaaagcggtc?gctgagcccg?tgacgccgtg 12420
aggtgtgccg?ggtcggtggt?gggccgaagg?ctcccttcgc?cgcgtctaat?gcggtgaagg 12480
gagctttcac?cccggtcact?cacccgcgcc?ggccacctcg?ccgacggcgc?ccgtgtcgcg 12540
aaagccactt?tcgcgacgcc?gggccgcgtt?tgccttaccg?ctcaacagaa?cggcgcggct 12600
ggacatgttg?tccggcaagg?ccggaccggg?ggatgatccc?ttcactgggg?ttattccgta 12660
caagaaggcg?cactcctcga?accggccaag?gccgaatcgg?ggatttcaag?ctgagctaga 12720
cgaacttcac?acccttcgct?gattgaggga?gacattgatg?aaccagtcgc?ggattgagga 12780
aatctggccg?ctgtcgccat?tgcaggccgg?attgctcttc?cacgcggttt?acgacggcga 12840
agggcctgac?gtctacgtcg?gtcattggat?cctggacctg?gacgggccgg?tggacgcggc 12900
caggctgcgc?gcggcctggg?aagcgctgct?gtcccggcac?gccccgctgc?gcgcttgttt 12960
ccggcagcgc?aaatcgggcg?agacggtgca?gatcatcgcc?aagcaggtgg?accttccgtg 13020
gcgggagatc?gacctctcgc?aggtcgaaga?tcccgccgaa?gccgtgcgtg?aactggccga 13080
gcaggaccgg?acgaccagat?tcgatctgac?gcgcgcgccc?ttgctgcggc?tcactttgat 13140
ccgcctcggc?gacgacgcgc?accgcctggt?ggtgacctgc?catcacacga?tcatggacgg 13200
ctggtcgctg?cccatcgtgc?tggccgagat?gtcgacgctg?tacgcggcgg?acggcgctcc 13260
gacggacctg?ccggaagtga?cgtcgtaccg?ggagtacctg?gcctggctgg?gccgtcagga 13320
caaggagcgg?tcgctcgcgg?cgtggacgga?acggctggcg?ggcgccgagg?agccgacgct 13380
ggtggtgcct?gcggatccgg?ggcgggcgcc?ggccgagccg?gagagtgtcg?aggccgggct 13440
gccggagaac?gtcacgcgag?cgctgggcga?actggcccgt?gccaagggat?tgacgctgaa 13500
cacggtggtg?cagggcgcgt?gggcactggt?gctggcgcag?ctgaccgggc?ggacggacgt 13560
ggtgttcggg?gcggcggtgt?cggcgcgtcc?gccggacctt?cccggcgtgg?aggggatggt 13620
ggggctgttc?ctcaacaccg?ttcccgtgcg?cgtgagcttg?caggcttcga?cgccggtcgc 13680
cggattgctg?acggagctgc?agaagcggca?gtccgcgctc?atccccgacc?agtacgtggg 13740
gctggcggac?atccagcagg?cggccggtcc?cggcgccgtc?ttcgacacgt?tgatcgtctt 13800
cgagaagttc?caccacgatt?ccggggaatt?gggctccgcc?gagaccttcg?gcgtccgggt 13860
gcatcagggc?cgcgtggccg?cccactatcc?gctgacgctg?gtcgcggtcc?ccggcgagtc 13920
gatgtacttc?aagctggact?atctgaccga?cctcttcgac?cgggacaccg?cgctcgcgat 13980
catcgagcgt?ttcaccgggg?tgctgcgcct?gctgacggac?gcgggcgagg?ccacggtcgc 14040
cgacatcgag?gtgacgagcc?cggccgagcg?cgccttggtg?gtcgacgcct?ggggcacgtc 14100
gtcccaggcg?gcgccgaacc?ggacggcgcc?ggaactgttc?gacacacagg?tggagcgcca 14160
ccgcgacgag?ccggcgatcg?tcgacggcga?ccgggcgctg?tcgtacggag?aactcgccga 14220
gcgcgcggaa?cggctcgccg?gtcacctgca?cggcaaggga?gtccggcgcg?gcgaccgggt 14280
ggccgtggtg?ctggaccggt?cacccgagct?gatcgtgacg?ttgctcgcgg?tgtggaaggc 14340
gggcgcggcg?tacgtgccgg?tggatcccgc?ctatccggtg?gaacgcgtgc?agttcatgct 14400
gacggattcc?gggcccgcgg?cggtggtgtg?cgcgaaaacg?taccgggacg?ccgtgctgga 14460
cgtcggcctc?gaaccgatcg?tgctggacga?tccccggacg?cggcgggcgg?tggaggagag 14520
cgctcgccgg?tcgaccgcca?cgagcggcct?cgacctcgcc?tacgtgatgt?acacgtccgg 14580
gtcgacgggg?acgccgaagg?gggtcgcggt?gtcgcacggc?gacgtcgcgg?cgctggccgg 14640
ggaaccgggc?tggcgggtgg?gccccggcga?cgcggtgctg?atgcacgctt?cgcacgcctt 14700
cgacatctcg?ctgttcgagc?tgtgggtgcc?gctggtgtcg?ggcgcacggg?tggtcttggc 14760
cggagcgggc?gcggtggacg?gcgaggcgct?ggcgcgtcac?gtcgccgacg?gcgtcacggc 14820
cgcccacttg?accgcggggg?ccttccgcgt?gctggccgag?gaatcgccgg?aatccgtcgc 14880
cgggctgcgc?gaggtgctga?ccggcggcga?cgcggtgccg?ctttcggcgg?tggagcgcgt 14940
gcggcgggtg?tgcccggacg?tgcgggtgcg?gcatctctac?ggcccgaccg?aggccacgct 15000
gtgcgcgacg?tggtggctgc?tcgaacccgg?ggacgagccg?ggttcggtgc?tgccgatcgg 15060
acggccgctc?gcgggacggc?gggtctacgt?gctcgacgcg?ttcctgcggc?ccgtgcccgc 15120
gggggtgccc?ggcgagctgt?acctcgccgg?agccggtgtg?gcgcaaggct?atctggaccg 15180
tccggcgttg?acggccgagc?ggttcgtcgc?cgatcccttc?gcggccgacg?ggcggatgta 15240
ccgcaccggg?gacctggcgt?actggacgga?tcagggcgcg?ctggcgttcg?ccgggcgcgc 15300
cgacgaccag?gtgaagatcc?gcggctatcg?cgtggaaccg?ggcgagatcg?aggtggtcct 15360
cggcggtctg?ccgggggtgg?cgcaggccgt?ggtgctcccg?aggggcgagc?atctgatcgg 15420
ctacgtggtc?gccgaagcgg?ggcaggacgc?cgacccggag?cgcctgcgcg?ggcagctcgc 15480
cgagaccttg?cccgaattca?tggtcccggc?cgtggtgctg?gtactggacg?ccttgccgtt 15540
gacggtcaac?gggaaggtgg?accggcgggc?cttgcccgaa?ccggacttcg?cgtcgaagtc 15600
ggcgggccgg?gaaccggtca?ccgacgccga?acgagtcctt?tgtggagtgt?tcgccgaagt 15660
cctcggtctc?gaccgggtcg?ggatcgacga?cagcttcttc?gaactgggcg?gcgactcgat 15720
ctcgtcgatg?caggtcgccg?ctcgcgcccg?tcgtgagggc?atcccgctga?cgccgcggca 15780
ggtgttcgag?caccggactc?ccgagcgcct?cgcggtcttg?gcgcgggaga?ccgccgcgac 15840
gccgcgcgcc?gaggccgtca?ccggcgtggg?ggagatcccg?cggacgccgg?tgatgctcgc 15900
gctcggagac?gacgcgctgc?gccccggttt?cgcgcaggtg?cgcgtcgtcg?tcgctccggc 15960
gggcctggac?caggacacgc?tggcgagcgc?gctgcaggcg?gtgctggaca?cgcacgatct 16020
gctgcgggct?cgggtggagc?cggacggacg?gctgatcgtg?cccgagcgcg?gcgcggtgga 16080
cgcggccggt?ctggtcacgc?gggtggccgc?tggaactgcg?gaactcgacg?aggtcgccga 16140
gcgcgagctc?aggacggcga?cgggcacgct?ggacccgtcg?gcggggatca?tggcgcggct 16200
cgtctgggtc?gacgccgggg?acgccgggcc?gggacggctg?gccttcgtgg?cgcatcacct 16260
ggccgtcgac?gcggtgtcgt?ggggaatcct?gctgccggat?ctgcggacgg?cttacgacga 16320
gctgatctcc?ggccggaccc?ccgccctcga?agcaccggcg?acgtcgtacc?ggcagtgggc 16380
gctgaggcag?gtcgagcagg?cgcacagcga?gcgcaccgtg?gccgaactcg?aggactgggc 16440
cgccctgctg?gacggcgcgg?acccggccgg?ggaagaggac?accggacagt?cgtattcgtg 16500
gtccacgcga?ctgaccggca?ccgaggcgcg?caacctggtg?acgctcttgc?cgggcgcgtt 16560
ccactgcggg?atccaggacg?tcctgctggc?gggactcgcc?ggtgcggtgg?cgcgggtacg 16620
cggtaccggc?gccgggctgc?tggtggacgt?ggaaggacat?gggcgcgaag?ccgccgacgg 16680
cgaggacctg?ctgcgcaccg?tcggctggtt?caccagcgtc?cacccggtcc?gcttcgatct 16740
gtccgatgtg?gacctcgcgg?ccgccggatc?gttgctgaag?accgcgaagg?accagatccg 16800
cgccgtgccc?ggcgatggat?ccggctacgg?gctgctgcgt?catctcaacc?ccggcaccgc 16860
ggcgacgctg?gccgcgctgc?cgtccgcgcg?gatcggcttc?aactatctcg?gccggaccgt 16920
cctcgccccg?gaggacaccg?cgtgggagcc?ggacggcgaa?gggccgctcg?gcggcggtcc 16980
ggacatggtc?ctcgcgcacg?ccgtggaggt?cagcgcggag?gtccaggaca?cgccgtccgg 17040
accccggctg?gggttggcca?tcgacgcccg?ggacgccggc?tccggcctgg?tgcaacggct 17100
cggtgaggcg?tgggcggaga?cgctgagcgc?cctcgcggcg?ctcgccgggg?atcccggcgc 17160
gggcgggcac?acgccgtccg?acttcgccct?cgtcggcctg?acgcggcggg?acgtgacgga 17220
actggaaacg?gcggcgccgg?ggctgaccga?catctggccg?ctgtcgccgt?tgcaggaggg 17280
catgctcttc?gagcgggcct?tcgacgccga?cggcgtcgac?gtctaccaga?gccagcggat 17340
cctgggcctc?gacgggccgc?tcgacgcgga?acggctgcgc?gaggcttgga?cgcgggtgct 17400
cgcccggcac?gaatcgctcc?ggaccggttt?tcatcagctg?gggtccggcg?agacggtgca 17460
ggtcgtcgtg?ggggaggccg?aactcccttg?gcgcgtggtg?gatctgtcgc?acctcgacga 17520
ggcggccgcg?gccgcggaga?tcgaacggct?cctcgccgag?gatcaggaca?ggcggttcga 17580
cgtgacccag?ccgccgttgc?tgcggctgtc?gctgatccgg?ctcggtgccg?accggcaccg 17640
gctcgtgctg?acgtcccacc?atgtcgtgct?ggacggctgg?tcgacgccgc?tcgtcgtggg 17700
tgaggtgtcg?gccgcgtacg?cgggcgggca?gctccccgcg?aaggcgccgt?ccttcaagga 17760
ctacctggcg?tggctgggcc?gccaggacgc?cgacgcgacc?cgggcggcgt?ggcgggccga 17820
gctcgacggc?gcggacgaac?cgtcccttgt?ggacgccgac?gcggacaaga?cgatggtgat 17880
gcccggggag?cacgccgaac?tgctgtccga?ggggacgacg?cgcgcgctca?ccgaactggc 17940
cagggccaac?ggggtgacga?cgagcacgat?cctgcaggcc?gcctgggcgc?tggtgctggc 18000
gcggctcgcg?ggccggacgg?acgtggtgtt?cgggtcggtg?gtggccggcc?gtccggcgga 18060
ggtgccggac?gtcgaacgca?tggtggggat?gttcatcaac?accgtcccgg?tccgggtgcg 18120
gctcgacggc?gggcagccgg?tgctggagct?gatccgggag?ctgcagggac?gcctggcggc 18180
gttgaccgaa?caccagtacc?tcggcctgcc?ggagatccag?aaggcggtgg?gcacgggcgc 18240
ggtcttcgac?accatcgtca?tgatcgagaa?ctacccgcac?accgccgacg?gtctcggcga 18300
cgacggcggg?gtcgcgatca?gctcggtcgt?cacccgcacc?ggcaccggtt?atccgctgac 18360
catgaacgtc?agcctcggga?gccgcctgcg?gatcaacgtg?tcctaccggc?ccgaccggat 18420
cggcgccggc?acggccgccg?aggtcgcccg?gcaggtcgtg?cgggtcctgg?accaggtggt 18480
ggcacggcct?tcgctgccgg?tgggccgcct?cggcgtgacg?agcgaaccgg?cgcgcgagtc 18540
ggtggtggaa?cgctggaacg?cgacgggcga?cgcggccgac?acgacgtcgg?tactggagtt 18600
gttccggcgt?caggccgtcg?cctcgccgga?cgcggtggcg?gtcgtggacg?gggaacgcac 18660
cctgtcctac?accggcctcg?accgtgaatc?cgaccggctg?gccggatatc?tggccgccgt 18720
cggggtgcgg?cgcggtgacc?gcgtcggtgt?ggtgatggaa?cgcggcgcgg?acctcctcgt 18780
cgccctgctc?gcggtctgga?aggcgggtgc?cgcgcaggta?ccggtgaacg?tcggctaccc 18840
cgcggagcgg?atcgagcgga?tgctggccga?cgccggggtg?tcggtcgcgg?tctgcctcga 18900
agcgagccgc?aaggcggtgc?cggacggggt?cgagccggtg?gtcgcggatc?tgccgtcgct 18960
cgacgggaca?cggcacgagg?cgccacgggt?cacggtcggg?gcgcacgacg?tcgcctacgt 19020
catgtacacg?tcgggatcga?ccggggtgcc?gaagggtgtc?gcggtgccac?acggaagcgt 19080
ggcggcgctg?gcgagcgacc?cgggctggtc?gcagggcccc?ggcgatcacg?tactgctgca 19140
cgcgtcgcac?gctttcgacg?cgtcgctggt?cgagctctgg?gtgccgctgg?tcagcggggc 19200
ccgcgtggtg?gtcgcggaac?cgggcaccgt?ggacgcggag?cggctccgcg?aggcgatcgc 19260
ccgcggggtg?accaccgtcc?acctgacggc?gggtgccttc?cgtgcggtgg?ccgaggaagc 19320
gccggactcc?ttccgcgggc?tgcgggagat?cctgaccggt?ggggacgcgg?tcccgctcgc 19380
ctccgtcgaa?cggatgcgcc?gggcctgccc?cggcgtccgg?ttccggcagc?tctacggtcc 19440
gaccgagatc?accctctgcg?ccacctggct?cgttctcgaa?ccgggggcgg?aaacggggga 19500
cacactgccg?atcggcggac?cgctggcggg?tcggcgggcc?tacgtgctcg?acgcgttcct 19560
gcagccggtg?gccccgggtg?tgaccggtga?gctctacctc?gccggagcgg?ggctggcgca 19620
cggctacctc?ggcaacccgg?tgtcgacttc?ggagcggttc?gtcgccgatc?cgttcgcccc 19680
cggagagcgg?atgtaccgga?ccggcgacct?ggcacgctgg?acggaacagg?gccagctgct 19740
cttcggcggg?cgtgccgacg?cccaggtgaa?gatccggggc?taccgggtcg?aacccggcga 19800
gatcgaggcg?gtgctgaccg?agatccccga?cgtcgcgcag?gcgatcgtgg?tggcgcggga 19860
ggaacgcccc?ggcgaaaagc?ggctgatcgc?ctacgtgacc?gcggaaccgg?gacagggact 19920
ggaatccgcc?gcggtccgcg?cgcagctcgc?ggcccggctg?ccggagttca?tggtgccgtc 19980
ggtggtgctg?gtgctggacg?gtttcccgtt?gaccatcaac?gggaagatcg?accgcgcggc 20040
cctgcccgcc?cccgaattcg?tcccggcggc?ggccggtcgc?gaaccgcgca?ccgggaccga 20100
acgggtgctg?tgcgacctgt?tcgccgaaat?cctcggactg?gaccgggtcg?gcgccgacga 20160
cggtttcttc?gaactgggcg?gggactcgat?cctctcgatg?cggctggccg?cccgcgcgcg 20220
ccgcgagaac?ctcgtgttcg?gggcgaagca?ggtcttcgac?cacaagacgc?ccgcggggat 20280
cgcggcggtc?gccgagcacg?gcggacggac?gcgggccgag?gacggcgagg?gcgtcggcga 20340
ggtcccgttg?acaccggtgg?tgcgggcgct?gctcgaacgc?gaccccctcg?gactgacccg 20400
cggcggcatg?gcgcagtggg?tgatcgtgga?cgtgcccggc?gacctttccg?tgaccgccct 20460
ggccgccgga?ctgggcgcgg?tgctcgacac?ccacgacctg?ctgcggagcc?ggctcgtcgc 20520
gaccgagggc?gaggagcccc?ggctggtcgt?ggccgaccgg?ggaaccgtcg?acgcggcgac 20580
gctggtcgag?cgtgccgccg?cgggcgacat?cgacgaaatc?gcgagtgacg?cggcggcacg 20640
cctggatccc?ctcgcgggcg?tgatggtgcg?ggcggtcctg?gcggacgccg?ggcggctcgt 20700
ggtggccgcg?catcacctcg?tggtcgacgt?ggtgtcgtgg?cgaaccctgc?tgccggacct 20760
gcaagcggcc?tgcgaggccg?cagtcgccgg?cctgcgtccg?tcgctcgaag?cgccggacgt 20820
ctccttccgg?cgctggtcgc?ggacgctgac?cgaagaggcg?gcgaaccgga?ccgccgagct 20880
ggcgacgtgg?acggagatcc?tcgacggggc?accgcggcgg?ctcggcgagc?tcgatccccg 20940
gcgtgacacc?gtgtcgaccg?cgggccgccg?gacctggacc?cggccggcgg?gcacgctcgc 21000
ggacaaggcg?acctcggcct?tccactgcgg?tgtccacgag?gtactgctgg?ccaccctggc 21060
cggcgccgtg?accggctggc?ggggcggcac?cgccgtcgtc?gtggacgtcg?aaggccacgg 21120
gcgtcagccc?ctcggcgaca?tggacctgtc?gcggacgctc?ggctggttca?ccgacgtcca 21180
cccgctccgg?ctggacacga?ccggggccga?cccggccgac?gtcgtcgcgg?gcggcgacgc 21240
ggcgggccgt?ctgctgaagc?aggtcaagga?gaacgtgcgg?gcggtgcccg?acggcgggct 21300
cggctacggg?atgctgcgtc?atctcaaccc?ggagacggca?ccggtcctcg?cggcgctgcc 21360
gaaggcggag?atcggattca?actacctcgg?ccgcttctcc?gcacgagccg?acggtggcgg 21420
cgagccttgg?cggataacgg?gaatcgtcgg?tgacgcggcg?gacgatgcca?cgcctctgcg 21480
gcacgtcctg?gagatcgacg?ccgtcgtggc?ggacggcgcg?gacggtcccg?agttcagcct 21540
gaccgtgacc?tgggcccgcc?ggctcctcgg?cgacgccgaa?gccgagtcgt?tcgccgacgc 21600
gtggctggcc?gcgctgaccg?gcctcgccgc?gcacgtcgac?ggcggcggcg?ggggacacac 21660
tccgtccgac?ttcccgctca?ccgcgctgac?gcagcgagag?gtggcgcgga?tcgaggccgc 21720
cgtgccggat?ctgctcgaca?tctggccgct?ttccccgctg?caggagggat?tgctgttcca 21780
ctccaccggc?gaggacggcc?tcgacgtcta?cgcgagcatg?cggaccctcg?ccctcgacgg 21840
cccgctggac?gccgcccggt?tccgcgcgtc?ctggcaggcc?atgctcgacc?ggcacgccgc 21900
cctgcggacg?agtttccacc?agctcccgtc?cgggacggcc?gtgcagacga?tctcccgtga 21960
ggcgacgctg?ccctggcagg?agaccgacct?gtcccggctg?cccgaggacg?aggcgctggc 22020
ggaattcgac?cggctcgccg?cggcacagca?cgccgaacgg?ttcgacctgg?ccaagggacc 22080
gatgctgcgc?ctgcacctgg?tgcgcctcgg?cgaacgcagg?caccggctga?tcttcacgtc 22140
gcatcacatc?gtggtcgacg?gctggtccct?gccgctgctc?gccaccggcg?tgctggccgt 22200
gtacgaagcg?ggcggcgacg?cccgggcgct?gcccccgtcg?gcgtcgtacc?gcgactatct 22260
cgcctggatc?gcggaccagg?acaagacggc?ggcgctggag?gcgtggcgga?ccgaactggc 22320
ggggctcgac?gaggcgaccc?agatcgtgcc?gccggagtcg?atcctcaccc?cgctcgcacc 22380
cgaacgcgtc?aggttcgccc?tcggcgacga?gccgagcagg?cgcctggcgg?agttctgccg 22440
ccgtcacggg?gtcacgccga?acacgctctt?ccaggggatc?tgggccctgc?acctggcccg 22500
gctgaccgga?cggcgggacg?tggtcttcgg?cgccgcggtg?gccgggcgtc?cggccgacat 22560
ccccggggtg?gaggccgcgg?tcggcctgtt?catgaacatg?ctgccggtcc?gggcgcgcct 22620
cgtcggcacc?gaaccggtcg?tcgacatgct?gaagaacctg?caggagcggc?agttcgatct 22680
gctgccgcat?cagcacctcg?ggcagggaga?gatcatcaag?gcggcaggcc?ggggcgcggc 22740
gttcgacacg?atcctggtgt?tcgagaacta?tccgcccccg?ccgccccggc?tcgacggccc 22800
cgacgcgctc?gtcttgagcc?ccgcggggaa?ctcggacgac?gacgggcact?atccgttgtc 22860
cctgcgtgct?tccgtggcgg?gaggtccggt?ccgcggtgaa?gtgatctacc?ggccggacgt 22920
gctcgaccgg?gccgaggccg?aggagacggt?cgcgaccctc?ctccgcgcac?tcgagcaggt 22980
gctggccgaa?ccgcggacgc?cggtggggcg?gatcggcctg?atcggcccgg?agcagcgccg 23040
cctggtggtg?gacgagtgga?accggaccgg?caggccggtc?accgagaaga?cgctgccggt 23100
gctgctgcgc?gaacaggcgg?agcggacgcc?ggacgcgatc?gccgtccagg?acgcggcgcg 23160
aggcctgtcg?taccggggat?tggtcggcga?agtggagtcg?ctggcgcggc?tgctcgccga 23220
ggcgggcgtg?cgacgcgaag?cccgggtggg?ggtgctggtc?gagcgttcgg?ccgacctcgc 23280
ggtgaccctg?ctgggggtca?tgttcgccgg?cggggcgttc?gtgccggtcg?atccggacta 23340
tccgcgtgac?cgcgtcgaat?tcatgctggc?gaactcggcg?ccaggggtgc?tggtgtgcac 23400
gaaggcgacc?cggtcgttcg?tgcccgcgga?gttcgcgggc?acggtgctgg?tgctggagga 23460
cctgcccgcc?gccggtccgg?acgccgccct?gcccgagccg?gcgccggaag?acgcggcgta 23520
cgtgatctac?acgtccggct?cgacgggcgt?gccgaagggt?gtcttggtca?cccactccgg 23580
actcgtcaac?ctcggctacg?cgcatatcga?acggatggcg?gtgacgtcgt?cctcacggat 23640
cctgcagttg?tccgcgatcg?gcttcgacgc?catggtgtcc?gagctctaca?tgtccctgct 23700
ggcgggggcg?accctggtcc?tgccggacgc?ggcgagcatg?ccgccgaagg?tgacgctggg 23760
cgacgcgatc?cggcaggcgg?agatcacgca?tctgaccgtg?tcgccgagtg?tgctggcgag 23820
cgaggacgac?ctgccggaca?cgctgcggac?ggtgctgacg?ggcggcgagg?cgctcccggc 23880
cgcgctggtg?gaccgctggt?cgccgggccg?ccgggtgatc?caggcctacg?ggccgaccga 23940
gaccaccatc?tgctcgacga?tgagcgcccc?gttgtccccg?ggacacgacc?aggtcccgct 24000
cggcggaccg?atccacaacg?tggggcacta?cgtgctcgac?gcgttcctgc?ggccggtgcc 24060
gcccggtgtg?gtcggcgagc?tctacatcac?cggtgtcgga?ctggcccgcg?gctacctcgg 24120
ccgtcccggc?ctgaccgcgg?aacggttcgt?cgccagcccg?ttcgcgcccg?gcgagcggat 24180
gtaccgctcg?ggcgacctgt?tccgctggac?ccgggaaggc?cagctgctct?tcgccggtcg 24240
ggtcgacgcg?caggtcaagg?tccgcggcta?ccgggtcgag?cccgccgaga?tcgaggccgt 24300
gctcgcggag?catccgtggg?tcggccaggt?ggcggtctcc?gtccgccggg?acgggccggg 24360
cgacaagcgg?ctggtggcct?atgtcgtgcc?gtcggcggcc?gccggcgagg?ggaacggcac 24420
gctggccgcc?gcgctgcggg?agctggccgc?cgaacgcctg?ccggagtaca?tgatgcccgc 24480
ggcgttcgtg?tcgctggaga?agatgccgct?caccccgaac?ggcaaactcg?atcaccgggc 24540
gctgcaagcc?cccgacttcg?ccgggacgtc?ggcgaaacgg?gagccgcgca?cggccgcgga 24600
ggcggcgctg?tgcgcgctgt?tcgcggacgt?gctggggctc?gaccaggtgg?ctcccgagga 24660
cagcttcttc?gaactcggcg?gcgactcgat?cacctcgatg?cagttgtcgg?cgagggcccg 24720
ccgtcagggg?ctggaactga?ccccgtggca?ggtgttcgag?gagaagacgc?cggaacggct 24780
ggcgccgctc?gtccaggaac?tcccggccga?ggacgcgccc?gtcgcggtac?cggagcccgc 24840
cgacggcacg?ctactcgccc?tttcccctga?ccagatggac?ctactcgagg?ccgggctggc 24900
cggcgaatga?cccgcgttaa?ggagcagatc?gtgaccgtag?acgacactcg?cgcgaagccc 24960
cgctcgagcg?tggaggacgt?ctggcctctt?tcgccgctgc?aagagggaat?gcttttccac 25020
accgccctcg?acaaggacgg?gccggacacc?tacacggtgc?agaccgtcta?cgggatcgac 25080
gggccgctgg?acgcgggacg?gctgaaggcg?tcgtggcagg?cactcgtgga?ccggcacgcc 25140
gcgttgcggg?cctgtttccg?ctacgtcagc?ggcgcgcaga?tggtgcaggt?catcgcgcgg 25200
gacgccgaga?tcccgtggcg?ggagacggat?ctttcgggac?tggcggccga?cgccgtcgaa 25260
gccgaagtcg?acaggctggc?cgcggcggag?gtggccgagc?ggttgagcgt?cgagacggcg 25320
ccgctgatga?aactgcacct?gatccggctg?ggcgaggaga?gccaccggct?cgtgcacacc 25380
ctgcaccacg?tgctgaccga?cggctggtcg?atgccgatca?tccaccgcga?gctcgccgag 25440
atctacgccg?cgggaggcga?cgcgtccggg?ctgccgccca?ccgtctccta?ccgggactac 25500
ctcgcctggc?tgggacggca?ggacaaggaa?gcggcgaagg?cggcctggcg?gaacgagctc 25560
gccgggctcg?acaccccgac?cttggtcgtt?cccgccgatc?cggcgcgcgt?gccggatatc 25620
cacaccacgg?tggtcgagct?gtcggcggag?ctgaccgacg?gcctcgcccg?gctggcgcgc 25680
ggccacggtc?tcaccctgaa?ctccgtcgtg?cagggcgcgt?gggccgtcgt?gctgtcgcag 25740
ctcgccggtc?gcaccgacgt?ggtgttcggc?gcgaccgctt?cggggcggcc?cgccgatctc 25800
ccgggcgtgg?agtcgatggt?cggccagttg?ctcaacaccc?tgccggtccg?tgtccggctc 25860
gacggcgcga?agcgggcggt?cgacctgttc?gacgaggtgc?agcgcggcca?ggcggcgctc 25920
atggcgcacc?agtatctcgg?gctccaggac?gtgcggagcg?tcgccggacc?cggggcggtc 25980
ttcgacacgc?tcgtcatcta?cgagaacttc?ccccgcaagg?gactcgggaa?gccgtcgggt 26040
gacggcctga?acctgctccc?gctgaagcgg?gggagcaact?cctcgcacta?tccgttcacc 26100
ctcgtcaccg?gcccgggcga?acggatgccg?ctcatcctcg?actacgaccg?gggcctgttc 26160
gaccccgcgg?ccgccgaatc?ggtcgtcggc?gcgatggcac?gggtgctgga?gcggctggtc 26220
gccgagcccg?acgtcctcgt?cggccgtctc?accctcgcga?gcgaggccga?acgcgccctg 26280
gtggtggacg?gtttcaacgc?gaccgcgggc?ccggtgccgg?gggactccgt?cgtcgaactg 26340
ttcgcgcggc?gggtggccgg?ggcgccggac?gcggtggcga?tcatcgacgc?cggcggcgcg 26400
aacctgacct?acgccgaagt?cgatcaggcg?tcggatcggc?tggccgggta?tctcgccggt 26460
cgcggtgtcg?gccgcggcga?ccgcgtcggc?gtggccatgg?aaaggtcgcc?ggagctgccc 26520
atcgcgttcc?tggcgatctg?gaaggcgggc?gccgcctatg?tgccggtgga?catcgagtac 26580
ccggccgagc?gcatctcgtt?catcctcgcc?gactccggcg?tctcgaccgt?cttgtgcacc 26640
gaggccacga?aggcggtcgc?tccggaggac?gcgatcgtgc?tcgacgcgcc?cggaacacgc 26700
gcggccgtgg?acgggtgcgc?cgcaccggga?atccggccgc?acgcggacga?tctggcgtac 26760
gtcatgtaca?cctcgggatc?caccggcctg?ccgaagggcg?tggggattcc?gcacggggcc 26820
gtcgccgggc?tggcgggtga?cccgggctgg?cagatcggcg?aggacgacgg?cgtcctgatg 26880
cacgcgacgc?acgtcttcga?cccgtcgctg?tacgcgatgt?gggtgccgct?cgtctccggc 26940
gcccgggtcc?tgctcaccga?accgggcgtg?ctggacgcgg?ccggggtccg?gcaggccgtg 27000
cgacggggcg?cgacctacgt?ccacctcacc?gcgggcacct?tccgcgcact?ggcggaaacc 27060
tcgccggagt?gcttcgaagg?cctcatcgag?atcgggaccg?gcggcgacgt?ggtccccttg 27120
cagtcggtgg?agaacctgcg?gcgggcccag?cccggcctgc?gcgtgcgcaa?cacctacggg 27180
ccgaccgaga?ccaccctgtg?cgcgacctgg?ctgccgatcg?aacccggtgc?cgtgctcggc 27240
tccgagctgc?cgatcggcca?cccgatgacc?aaccgccgga?tctacgtgct?cgacgccttc 27300
ctgcgcccgg?tcgcgccggg?tgtggccggc?gagctgtaca?tcgcgggcac?ggggctggcc 27360
cgcggctacc?tgaacaaccc?cggcctgacc?gccgaccggt?tcgtggcgtg?cccgttcctc 27420
cccggtgagc?gcatgtaccg?caccggcgac?ctggcccgct?ggacccgcga?cggcgagatc 27480
gtgttcctcg?gccgcgccga?cgaccaggtg?aagatccgcg?gttaccgggt?cgagctcggt 27540
gaggtggagg?ccgtgctggc?cgcccagccg?ggagtggtcg?aggcggtcct?gctggcgcgt 27600
gaggaccagc?cgggcgaaaa?gcgccttgtc?ggctacttcg?tgtccgacgg?caccgacgcc 27660
gggccggagg?agatccggcg?gcggatgggc?ctggtcctgc?ccgactacat?ggtcccgatc 27720
gcggtcgtgg?cgctgcccgg?cctgccggtc?acccccaacg?gcaaggtgga?tcggcgggcg 27780
ctgcccgccc?cggacctcgc?gggacgctcg?tcggagaagg?cgccggagag?cgagaccgaa 27840
aaggtgctgt?gcgcgctgtt?cgccgagatc?ctcagcgtcg?agcaggtggg?ggtcgacgac 27900
gccttcaacg?acctcggtgg?cagttcggcg?ctggccatgc?ggctcgtcgc?gcggatccgt 27960
gaggaactcg?gcgaggacct?gcccatccgg?cagctgttct?cctcaccgac?gcccgcgggg 28020
ctggccaggg?ccctggccgc?gaagtcccgt?ccggtactgg?aagccgccaa?gcggccggat 28080
cgggtgccgt?tgaccgcccg?gcagctgcgg?gcctggctgc?tggccgatcc?tggcggggag 28140
acggcgggcc?tgaccacctc?ggtcgcgctt?cgcctgcacg?gcaagctgaa?cgtgcccgcg 28200
ctggtgacgg?cgctcggcga?cgtcgccgag?cggcacgagc?tcctccgcac?gacttttccc 28260
ggtgacgcgc?agagcgtgca?ccagctcgtc?cacgacaccc?cggcggccgg?gccggtcccg 28320
atcccggtga?ccgaggaggc?cctccccggg?ttgctcgccg?agcggcgcga?ggccgccttc 28380
gacctcaccc?gtgaggtgcc?gtggcggtgc?gatctgttcg?agctctcgga?aaaggagcac 28440
gtgctgcacc?tccaggtgca?ccggatcctg?gcggacgacg?attcgctgga?cgtgttcttc 28500
cgcgacctgg?cggccgccta?cggcgcgcgg?cgtgagggcc?gggtcccgca?gcgcgcgccg 28560
ttggccttgc?agttcgccga?ctacgcgctc?tgggagcggc?ggctgctgga?ggacgagaac 28620
gaaccgggca?gcctgatcaa?cgaacagttc?gccttctggc?gggaaaacct?ggccggtatc 28680
gacggcgaga?cggtgctccc?gttcgaccgg?ccgcggcccg?ccgtcccgtc?gcggcgggcg 28740
ggcaccgtcg?cgctgcggct?ggacgccggc?ccgcacgccc?ggctgacgga?ggccgcggag 28800
tcggcgggcg?tggaaacgtc?gcggctggtg?cacgcggcgc?tcgccatgct?gctggccaaa 28860
tacggagcgg?gcgaagacgt?ggtgatcggc?tcggcgctgc?cccgggacga?ggaactcttc 28920
gacctcgaac?cgatggtcgg?accgttcacc?cggccgctcg?ccgtgcgcac?ggatctctcg 28980
ggtgatccga?cccaccggga?ggtcgtcaac?cgggtgcaag?aagccttcca?ggcgaccgaa 29040
cagcatctgg?acgtgccctt?cgagcgcatc?gtcgagctgc?tcgaactgcc?cgcctcgctc 29100
tcccgtcatc?ccgtgttcca?ggtgggtttg?cgggtggacg?aggaggacat?cgacggctgg 29160
acggcggcgg?aactgcccgc?cctgcgcacc?ggtgtcgaac?ccggtggggt?cgaggccatg 29220
gagctggatc?tgtcggtcga?actcaccgag?cttttcgacg?acgacgacga?accgggcggc 29280
ttcgagggcg?tgctgcgcta?cgccaccgac?ctgttcgacg?aggccaccgc?cgagtcggtg 29340
gcccggcggc?tggtccgcgt?cctcgaacag?gtggcggagg?atcccgggcg?gcggatcagc 29400
gagctggacc?tcttccttga?cgacttcgaa?cgctcccgtc?cgcccatcgc?gccggcgcgc 29460
tgggccgggg?ccgtcccgca?ggtggtcgcc?gacgtggccg?gggacgggcc?gctcggcgcg 29520
ctcgtactgg?acgagcggtc?gcgccccgtc?gccccgggca?ccgtcggcga?gctctacgtc 29580
accggtcccg?cggtggacga?gggaccggcc?gatctcccga?ccgtgccctg?tccgttcggg 29640
gaggaggggc?accggatgct?gcggaccggt?ctgctcgcca?ggaagacgcc?cgccaagacc 29700
ctggtcgtcg?tcggcgagcg?gcggcggacg?agctcttcgg?tgaagacggg?cgacttcgag 29760
atcctgctgc?cgctccggac?gaccggggac?cggcccccgc?tcttctgtgt?ccacgcgagc 29820
ggcgggctga?gctggaacta?cgagccgctg?ttgcgtcacc?tcccgtcgaa?ccagccggtc 29880
tacggcgtcc?aggcccgcgg?tctcgccagg?accgagccgc?tgccgggcag?cgtcgaggag 29940
atggccgccg?actacctcgc?gcagatccgt?gccgtgcagc?cggaagggcc?gtatcacctg 30000
ctcggctggt?cgctcggcgg?ccgtatcgcg?caggcgatgg?ccaaactgct?ggaggaggaa 30060
ggggagaagg?tcggcctgct?cgccttgctc?gacgcctatc?ccgtctacat?gggacggaag 30120
acgaccgggg?cggcgagcga?ggaagaggcg?ctgtcgaagc?ggaaccaggc?ggacctcgac 30180
ctcgcgggcc?agctggtcaa?gggcgaggcc?gcccgtgccc?gcctggaggc?ggtcatgcgc 30240
aatctctggg?acgtcgggcc?gcgccacgtc?tcttccccct?tcgccggcga?catcctgctc 30300
ttcgtggcca?ctgtggaccg?tcccgagcat?ttgccggttt?cggtggcgaa?ggccagctgg 30360
aaggacttca?ccagcgggac?catcgagacc?cacgacgtcc?cgtccaacca?ctacgacatg 30420
gtgcaaaccg?cggcgctggg?ccagattgga?gccatcgtcg?ccgagaaact?ccggcagcgt 30480
ccggaaggtg?aaaggacaca?acgatgacca?accccttcga?caacgaagac?ggttcctttt 30540
tcgttctggt?caacgacgag?ggacagcact?cgctgtggcc?cgccttcgcc?gaggtgcccg 30600
cgggctggat?cacggtgcac?ggcgaggccg?gccgcaagga?atgcctcgcc?tacgtcgagg 30660
agaactggac?cgacctccgg?ccgaagagcc?tcatccagga?agcgggcgcc?tgagggcgcg 30720
tgtccagccg?ctcaacgcgg?acggttggac?acgccgcagg?cttcgatcac?cggctggatg 30780
aggcgaaagg?accttgtgca?gtgttcgagg?agaagaacgc?cctccggggc?acggagatcc 30840
accggagaga?gcgcttcgat?ccggggccgg?aactgcgctc?cctgatggcc?gagggccgca 30900
tgtccgtgat?ggagtccgaa?gacggcccag?acgggcggac?cgggtggctc?gccaccgggt 30960
acgaagaaac?ccgccaggtg?ctcggctcgg?acaagttcag?cgccaagctg?ctcttcggcg 31020
gcaccgcggc?gggccggatc?tggcccggct?tcctcaacca?gtacgacccg?ccggaacaca 31080
cgcggctgcg?ccggatggtg?gcgtcggcgt?tcaccttccg?gcgcatgcag?gacttccggc 31140
cgaagatcga?ggcgatcgtc?gaggccacgc?tggacgacat?cgaagccacc?ggcgggccgg 31200
tggacttcgt?gccgcggttc?gcgtggcccg?tggcgacgac?ggtgatctgc?gacttcctcg 31260
gcatcccgcg?tgacgaccag?gccgacctgt?cccgcgccct?gcacgccagc?cgggccgaac 31320
ggtcgggcaa?acggcgtgtg?gcggcgggga?acaagtactg?gacgtacatg?ggccagatcg 31380
cggccaagac?gcgccgcgat?cccggtgacg?acatgttcgg?cgccgtggtg?cgcgagcacg 31440
gcgacgacat?caccgacgcg?gaactgctgg?gcgtggccgc?gttcgtcatg?ggggcgagcg 31500
gggaccaggt?ggccaggttc?ctcgcggcgg?gggcgtggct?gatggtcgag?caccccgagc 31560
agttcgccgt?gctccgggac?aagccggaaa?ccgtcccgga?ctggctgaac?gaggtggccc 31620
ggtacctcac?cagcgacgag?aagaccactc?cgcgcatcgc?gcgggaggac?gtgtacatcg 31680
gtgaccagct?ggtgaagaag?ggcgacgccg?tcacctgctc?gctgctggct?tcgaaccgcc 31740
ggcagttccc?cgacccggag?gaccggttcg?acatcacccg?ggaaaagccg?gcgcacgtca 31800
ccttcgggca?cggcatccat?cactgcctcg?gcaggccgct?ggccgaaatg?gtgttccggg 31860
cggcgatccc?gggcctggcg?caccgctttc?ccaagctccg?gctggccgaa?ccggaccgcg 31920
agatcaaact?gggcccgccg?ccgttcgacg?tcgaatccct?gctgctcgac?tggtagcccg 31980
ggccgtttcg?ttgccgggta?aggcgaagcc?ggcgccgggc?ccggtccgcg?gactgcgttg 32040
cgaaagccac?tttcgcaacc?ttcaacgttg?cgaaagtggc?tttcgtaacg?tcggaccggc 32100
ccggtgtcgt?gtgactggag?ggacgacaca?cgtgactgga?tggacgacac?acgtgactgg 32160
agggacgaca?agcacgttca?ggcggacgga?tcggccgtgt?tggatgccgt?ccctccagtc 32220
acgtgtgtcg?tccgtcggat?cacaggtgcc?gtccggccag?tcacgggaaa?tcaccatctc 32280
agtggacggc?ccgcccatga?aagcgcatga?cacgttcgca?aaggagtttc?ccgttgagtg 32340
aggacgaccc?gcgcccgctg?cacattcgca?gacaggggct?ggacccggca?cccgaactgc 32400
tcgccgccgg?gccgctgacg?agggtcacca?tcggctccgg?agcggacgcc?gaaacgcatt 32460
ggatggccac?cgcgcacgcc?gtcgtccggc?aggtgatggg?cgatcacaag?agtttcagca 32520
cccggcgtcg?ctgggacccc?cgggacgaga?tcggcgggaa?gggcatcttc?cggccgcggg 32580
aactggtcgg?caacctgatg?gactacgacc?cgcccgagca?cgccaggctg?cggcagaagc 32640
tgaacccggg?tttcacgctg?cgcaagatgc?agcggatggg?gccgtacatc?gaacagatcg 32700
tgaccgaacg?gctggacgaa?ctggagcagg?cgggatcccc?cgcggatctg?gtcgggatcg 32760
tcgccgacaa?ggtccccggc?gccgtgctgt?gcgaactgat?cggtgtgccg?agggacgacc 32820
gcgccacgtt?cttgcagctg?tgtcacgcgc?atctcgacgc?ctcgcgcagc?cagaagagac 32880
gcgcggtggc?gggtgaggcg?ttctcccgct?acctgctggc?gatgatcgcc?agggaacgca 32940
aggaaccggg?cgaaggcctg?atcggagcgg?tcgtcgccga?gtacggcgac?gaagccacgg 33000
acgaggaact?gcgcggcttc?tgcgttcagg?tgatgctggc?cggcgacgac?aacatctccg 33060
gcatgatcgg?cctcggtgcg?ctggccctgc?tgcggaaccc?cgggcagatc?gccgcgttcc 33120
aaggcgacga?gcagcaggcg?caacgggcgg?tcgacgagct?gatccggtac?ctgacggtcc 33180
cgtattcccc?gactccccgg?atcgccatgc?gggacatcac?catcgcgggc?caggcgatca 33240
aaaagggcga?gagcgtcatc?tgttccctcc?cggcggccaa?ccgggatccc?gcgctggtgc 33300
cggatcccgg?acgcctcgac?gtcacccgcg?agtccgcgcc?gcatgtcgcg?ttcgggcacg 33360
gggtccatca?ctgcctgggc?gccgcactga?cccggctcca?gctgcgcacc?gtgttcacct 33420
tgctgtggcg?gcgttttccc?gagctgcggc?tggcggatcc?cgcgcaggaa?accaagttcc 33480
gcctgaccac?cccggcgtac?ggattgaccg?agctgatggt?cgcctggtga?tcggcggctc 33540
cgtccacgcg?aggtcgctgg?acgtgccgcc?cgattaccgg?tcaaattggg?cgtcgctgtg 33600
ttcgggcggt?cgaagccgaa?agacactttc?ccggtctacc?aaggagttcc?agtcggatgg 33660
ggcacggtat?cgaacaggtc?gcgccactcc?tggaggagcc?cgcgaacttt?cagctgagaa 33720
cgaactgcga?tccgcatacg?gacaatttcg?cactgagggc?gcacggcccg?ctcgtccgga 33780
tagccggcga?ttcctcggcc?cagctcggcc?gggatttcgt?ctggcaggcc?cacggctacg 33840
acgtcgtgcg?caggatcctc?ggtgaccacg?agaatttcac?gacgcgcccg?caattcaccc 33900
aggcgaaatc?cggggcgcat?gtcgaggccc?agttcgtcgg?gcagatctcg?acctacgatc 33960
cgcccgagca?cacccggttg?cggaagatgc?tgacaccgga?gttcaccgtc?cggcggatcc 34020
gccggatgga?gcccgcgatc?cagagcctcg?tcgacgatcg?cctcgacatg?ctggaggccg 34080
aggggtcgcc?cgcggacctc?cagggcctgt?tcgccgatcc?ggtcggcgcc?cacgccctgt 34140
gcgaactgct?cggcatcccc?cgcgacgacc?agcgcgagtt?cgtccggcgg?atcaggcgga 34200
acgccgatct?gagccgcggc?ctcaaggcgc?gggccgccga?cagcgcggcg?ttcaaccggt 34260
acctggacaa?cctcatcgcc?cggcagcgcg?cggatcccga?cgacggactc?ctcggcacga 34320
tcgtgcgaga?gcacggcggc?aacgtcacgg?acgaggagtt?gcggggcctg?tgcacggcgc 34380
tgatcctcgg?cggtgtcgaa?accgtcgcgg?ggatgatcgg?cttcggcgtg?ctcgccctcc 34440
ttcagcaccc?cggccagata?ccgttgcttt?tcgaaggccc?ggaaaaggcc?gaccgcgtcg 34500
tcaacgagct?ggtgcgctat?ctgtcccccg?tgcaggcgcc?gaatccccgg?ctcgccatca 34560
aggacgtggt?cgtcgacggg?cagctgatca?aagcggggga?ctatgtcctg?tgctcggttc 34620
tcatggcgaa?cagggacgaa?gcactgaccc?ccgatccgaa?cgttttcgac?gcgaaccgca 34680
ccgctgtttc?ggacgtcggg?ttcgggcacg?gaatccatta?ctgcgtcggt?gccgctttgg 34740
cccggtcgat?gctgcggacg?gcctatcaga?ccttgtggcg?gcgattcccg?gggctgcggc 34800
tggccgtgcc?cgtcgaagaa?gtgaaatacc?gcagcgcgtt?cgttgactgc?cccgatcaag 34860
taccggtcac?ctggtgacga?ccggcccggc?gaagtgacac?tcgacaaagg?aatggtggga 34920
aatgtcggtc?gaagatttcg?atgttgtggt?ggcgggcggc?gggccgggtg?gttcgacggt 34980
ggccaccctg?gtggcgatgc?agggacaccg?ggtcctgctg?ctggagaaag?aggtcttccc 35040
ccggtaccag?atcggtgagt?cgctgctgcc?cgccacggtg?cacggggtct?gccggatgct 35100
cggcgtcgcg?gacgagctgg?cgaattccgg?gttcccgatc?aaacgcggcg?gcacgttccg 35160
ctggggcgcc?cgtccggagc?cgtggacgtt?ccacttcggg?atctcggcca?agatggcggg 35220
ctcgacgtcg?cacgcctatc?aggtcgagcg?ggcgaagttc?gacgacatcc?tgctgaagaa 35280
cgccaagagc?aagggcgtcg?tcgtgcggga?aggctgctcg?gtcaacgacg?tcgtggagga 35340
cggcgagcgg?gtcaccggcg?cgcgctacac?cgacgcggac?ggcaacgcgc?acgaagtctc 35400
ggcccggttc?gtgatcgacg?cgtcgggcaa?caagagccgg?ctctacacga?aggtcaacgg 35460
ttcgcggaac?tactcggagt?tcttccgcag?cctcgcgctg?ttcggctatt?tcgagggtgg 35520
caaacggctg?cccgagccgg?tgtcgggcaa?catcctgagc?gtcgccttcg?acagcggctg 35580
gttctggtac?atccccctga?gcgacacgct?gaccagcgtc?ggcgcggtcg?tgcgccggga 35640
ggacgccgac?aagatccagg?gcgaccgcga?gaaggccctc?aacaccttga?tcgccgaatg 35700
cccgctgatc?tcggagtacc?tctcgaacgc?gaccagggtg?accaccggca?ggtacggcga 35760
actgcgggtg?cgcaaggact?actcgtacca?gcaggacagc?tactggcggc?ccgggatggt 35820
cctggtcggc?gacgccgcgt?gcttcgtgga?cccggtgttc?tcctccgggg?tgcacctggc 35880
gacctacagc?gcgctgctcg?cggcccggtc?gatcaacagc?gtcctcgcgg?gcgacctcga 35940
cgagaagacc?gcgctgaacg?agttcgaggc?gcgctatcgc?cgcgagtacg?gcgtcttcta 36000
cgagttcctc?gtctccttct?atcagatgaa?cgtcaacgag?gaatcgtatt?tctggcaggc 36060
caagaaggtc?acgcagaacc?agagcaccga?catcgagtcg?ttcgtcgagc?tgatcggcgg 36120
ggtgtcgtcc?ggcgagaccg?cgctgacggc?cgccgaccgg?atcgccgcga?acagtgccga 36180
attcgccgcc?gccgtcgaca?agatggcgac?gggcgacggc?gacgacatgg?tgccgatgtt 36240
caagtcgacc?gtggtcaagc?aggcgatgca?ggaggcgggc?caggtccaga?tgaaggcgct 36300
gctcggcgag?gacgccgaac?ccgagctgcc?gctgttcccc?ggcggcctgg?tgacttcgcc 36360
cgacgggatg?aagtggctgc?cgcaccaccc?ggcctgaaac?ctccgcacgc?cggcggtgcg 36420
ccggtggtcg?agcacgaaac?aggggtagcg?aaatgcgtgt?gttgttgtcg?gtgtgcggaa 36480
cccgcgggga?cgtcgagatc?gcggtgtcgc?tggcggtccg?gctgaaggcg?ctcggcgtcg 36540
ggacgcggat?gtgcgcaccg?cccgccgccg?ccgagcggct?ggccgaggtc?gaggtgccgc 36600
atgtgccggt?cggccttccg?cagcacatga?tgttgcagga?ggggatgccg?ccgccgcccc 36660
cggaggagga?gcggcggctc?gcggccatga?cggtcgagat?gcagttcgac?gcgatcccct 36720
cggccgccga?aggatgcgtg?gcggtggtgg?cggtcggcga?tctggccgcc?gcgaccggcg 36780
tgcggtcggt?ggccgaaaag?ctgggcctcc?ccttcttcta?ctccgtcccg?agcccggtct 36840
acctggcttc?cccgcacttc?ccgccgccct?atgacgagcc?gaccaccccg?ggcgtgaccg 36900
accaccggac?actgtgggaa?gagcgtgccc?accggttcgc?ggaacggtac?ggggagacgc 36960
tcaaccggcg?gcgggccgcg?atcggcctgc?cgccggtgga?ggacgtcttc?ggctacggcc 37020
acggcgaccg?gcccatcctg?tcggcggacc?cggtcctcgc?cccgctgcag?ccggacgtcg 37080
acgccgtgca?gaccggcgcg?tggatcctga?ccgacgaccg?gccgcttccc?ccggagctgg 37140
aggcgttcct?ggccgccggc?ccaccgccgg?tgcacgtggg?tttcggcagc?tcgtccggga 37200
aggggatcgc?cgacgccgcg?aagatcgccg?tcgaggtgag?ccgtgcccac?ggccgccggg 37260
tgatcctctc?ccgagggtgg?accgatctgc?tcctgcccga?cgaccgggag?gactgtttcg 37320
ccatcggcga?ggtgaacttc?caggcgctgt?tcccccgggt?ggccgccgtc?atccatcacg 37380
gcagcgcggg?cacggaacac?gtggccacgc?gggcgggcgt?cccccagctc?gtgatccccc 37440
ggaacaccga?ccagccgtac?ttcgccgcca?gggttgccga?tctggggatc?ggtgtggcgc 37500
acgacggccc?gacgccgacc?ttcgagtccc?tgtcggccgc?gctcaccacg?gtcctggcgc 37560
cggaaacgcg?cgcgcgggcg?cgggccgtgg?cggccatggc?ccagaccgac?ggcgcggcgg 37620
cggccgcgga?tctggtgctc?gccgccgtcg?gcgggaacga?gcccgccgtt?cccgcgtgaa 37680
ccatcagcga?ccggacgccg?gccaggaccg?gagaaaacgg?ggaaatacgt?gatgcgtgtg 37740
ttgttgtcga?cctgtgggag?ccgcggagac?gtcgaaccac?tggtggcgtt?ggcggtgcgg 37800
ttgcgggagc?gcggcgccga?ggtgcggatg?tgcgcgccgc?cggactgcgc?ggatcggctg 37860
gccgaagtcg?acgtgccgca?tctgcccctc?ggtgcgtcgg?cgcgcccgtc?ggccgggcag 37920
gcgaaaccct?tgacggccga?ggacatgctc?cggttcacga?ccgagacgat?cgccacgcag 37980
ttcgagcgga?ttccggcggc?cgccgaagga?tgcgccgcgg?tggtgacgac?cggcctgctg 38040
gccgccgcca?tcggcgtgcg?gtcggtggcc?gaaaagctgg?gcatccccta?cttctatggc 38100
ttccactgcc?cgagctatgt?gccgtcgccg?tactatgcgc?ctccgccgcc?cctcggcgag 38160
ccgcccgcac?cggacgggac?cgacatccag?gcgctgtggg?agcgcaacaa?ccagagcgcc 38220
taccggcggt?acggggagcc?gctcaacagc?aggcgcgccg?ccatcggcct?gccgccggtg 38280
gaggacatct?tcggccacgg?ctacaccgat?cacccgtgga?tggcggcgga?cccggtactg 38340
gccccgctgc?aacccacgga?tctcgacgcc?gtgcagaccg?gggcgtggat?cctgcccgac 38400
gaacgaccga?tttccgctga?gctggaggcg?ttcctggacg?ccggcgcacc?accggtgtac 38460
ctggggttcg?gcagccttcg?cgcccccgcc?gacgccgcga?aggtggccat?cgaggcgatc 38520
cgtgcccacg?gccaccgggt?gatcctctcc?cgcggctggg?ccgatctggt?cctgcccgac 38580
gaccgggagg?actgtttcgc?catcggcgaa?gtgaatcagc?aggtgctgtt?ccgccgggtg 38640
gccgccgtca?tccaccacgg?cggcgcgggc?acgacccacg?tggccacgcg?ggcgggcgtc 38700
ccccagatcc?tggttcccca?gatcgcggac?cagccctact?acgccgcccg?ggtggccgaa 38760
ctgggggtcg?gtgtggcgca?tgacggcccg?accccgacct?tcgacacgtt?gtcggcggcg 38820
ctcaccaagg?ccctcgctcc?ggaaacgcgc?gtgcgagcgg?aagccgtggc?ggaaacggtc 38880
cagacggacg?gggccgcggt?ggccgcggac?ctgttgttcg?ccgcggtgac?cgggaaccag 38940
cccgccgttc?ccgcctgatc?atcaccagta?cagaggagac?cgcatgtcga?ccacttcccg 39000
gtgccgcatc?tgcgacggca?ccgtcaccga?gttcatcgat?ctcggacgcc?agccgctttc 39060
ggacgcgttc?gtggttcccg?gcgacgagaa?gaaggagttc?ttcttccggc?tcgccacggg 39120
gatctgcgag?tcctgcacga?tggtgcagct?gatggaagag?gtcccgaggg?acctgatgtt 39180
ccacgaggcg?tacccctatc?actcgtccgg?ttcgtcggtc?atgcgcacgc?atttccagga 39240
actggccaag?cgcctgctga?ccacggagct?gaccggcgag?gacccgttca?tcgtcgagct 39300
ggggtgcaac?gacggcatca?tgctcaaggc?cgtggccgaa?gccggggtac?gccagctcgg 39360
tgtggaaccc?tccggcggcg?tcgcggatct?ggccgcggcc?aagggaatcc?gcgtccgcaa 39420
ggatttcttc?gaagaggcga?cggcggccga?catccgggag?aacgacggcc?ccgccgacgt 39480
gatctacgcg?gccaacaccc?tgtgccacat?cccttacatg?gactcgatcc?tcaagggcgt 39540
cacgacgctg?ctgcgcccga?acggcgtgtt?cgtgttcgag?gacccgtacc?tcggcgacat 39600
cgtggagcgc?acgtcgttcg?accagatcta?cgacgagcat?ttcttcttct?tcaccgcgcg 39660
ctcggtccag?gagatggccc?ggcgcaacgg?tctcgaactc?gtggacgtcg?agcgcatccc 39720
ggtgcacggc?ggcgaggtcc?gctacaccct?cgccctcgcc?ggggcccgta?agccttcctc 39780
ggcggtggcg?gaactcctgg?cgtgggaggc?cgagcgcaag?ctggcggagt?acgccacgct 39840
ggaacgcttc?gccaccaacg?tgaagaagat?caaggaagac?ctgatcgcgc?tgctgaccaa 39900
gctccgcgcc?gagggcaagc?gcgtcgtcgg?ctacggcgcg?acggccaaga?gcgccacggt 39960
gaccaacttc?tgtggcatta?ccccggatct?ggtggagttc?atctcggaca?ccaccccggc 40020
caagcagggc?aggctcagcc?ccggacagca?catcccggtt?cgcgagtacc?aggcgttcgc 40080
cggcgaccac?ccggactacg?cgctgctgtt?cgcctggaac?cacgccgacg?agatcatgaa 40140
cgcggagcag?gccttccgcg?acgccggcgg?gcagtggatc?cggtacgtgc?cgaacgtgca 40200
cgtgagctga?tcgccatgac?ccgagacctc?ggcacggacc?ggatcctggc?gatctccccg 40260
cacttggacg?acgcggtcct?gtccttcggg?gcgggcctcg?cccaggcgga?acgggacggc 40320
gcgaaagtga?ccgtccacac?ggtgttcgcc?ggtaccgcga?cgcccccgtt?ctcaccggcg 40380
gcggaccggc?tgcactcgat?ctgggggctg?tcgccggacg?aggacgcgtc?gctgcgccgc 40440
cgtgacgaag?acatcgccgc?actcggccac?ctgggggccg?agtaccggca?cagccggttc 40500
ctcgacgcca?tctatcgcaa?ggcggcggac?ggccggtgga?tggccgacaa?cgtgccagga 40560
cggcagaaac?tggccatcgg?acggctgtca?ccgcaggacg?atccgctgtt?ctccgcggtg 40620
cgggaggaaa?tcgcgtcggt?cgtccaggag?tgcgacccga?cgctgatcct?gacctgcgcg 40680
gccgccagcg?ggcatatcga?caacgagatc?acccgggacg?ccacgctgct?cgtcgcgcgg 40740
gaaagggaga?tcccggtgcg?gctgtgggaa?gacctcccgc?acgcgatgtt?cgctcagggg 40800
gaagccgaac?tcccggaagg?attccggctc?ggcgcccccg?aattcgcctc?ggtcgaggcg 40860
gacgcgcggg?cgcggaaatt?cgaggcccta?ctgcgctatc?cgacgcagat?gcggatgctc 40920
cacgggccgg?acaaggatct?tttcgcgcag?ttggacgggc?acgccgggaa?gaacggcgtg 40980
cggtccgggt?acggcgaaat?gacctggccg?gtcgtcgtcc?gcgaagaaaa?cggctgaatc 41040
gggcctgaga?cgaatgtcga?cctggaggtt?gtcctgatga?ccgatcaact?ggaccgcggc 41100
ccggcgcgga?ccgcgcacgc?cgatgtcgtc?ctggcctcgg?cgggtgaccg?gggcgttctg 41160
tgcgatttct?acgacgaggc?gggctcggac?acctatcggg?atttgatcca?ggacgccgac 41220
ggcaccccgg?aaacgcggga?attcgcggcc?cgtcttcccg?aggatccggg?gcccgtgctg 41280
gagctggcgg?ccggaatggg?caggctgacg?ttccctttcc?tggagctcgg?ctgggagatg 41340
accgcactgg?agctgtcggc?gacggtggtc?accgctttcc?ggaagcggct?ggcggaatcg 41400
ccgtcggagc?tccgagagcg?gtgcacagtg?gttcaggggg?acatgagcgc?cttcgcgttg 41460
ggcgagcgtt?tcggcgcggt?ggtcatcagc?tcgggttcga?tcaacgaact?cggcgaggcc 41520
gaccggaagg?gcctgtacgc?gtcggttctc?gagcatctgc?ggcccggcgg?gaagttcctg 41580
ctcagcctgg?aattgacgaa?gcccgtcgaa?gcggagccgg?tggagcgcag?ccaggagctg 41640
accggccgga?gcggcaggag?gcacgtattg?cacgtgaaga?tcctgccgtc?ggaggagctc 41700
caggagatca?ccatccaccc?cgccgacgag?accgcggatc?ccttcgtcgt?ctgcacgcat 41760
cgccggaggc?tcttcacggt?ggaccggatc?gcgcgggaac?tcgggcaggc?cggcttcgac 41820
gtcgtcgcgc?gggaaccgtt?cgcctccacc?ggcaccggtc?gtgacgacat?ggtgctggtg 41880
gaggcgatcc?cgcgagaggt?cacggcagac?gcgaagtgat?gagcgcggcg?agcctgctca 41940
cgccctcctc?gatcagctcc?ggcgtgagca?ggctgatgga?cagccggagc?tggttgaacc 42000
cgtccttccc?gccgtagaag?tgatgcatcg?gggtgaacag?cacaccgtgg?tcgcgggcgg 42060
cgtccgccag?cagctcgtcg?tcgacgatga?agggcacggt?gacggtgacg?aagaacccgc 42120
ccgtcggggt?gttccagcgg?accccggggc?ggtcgccgag?cctgcggtcg?agctcgctca 42180
gcgtcagctg?gagattccgc?tggtagatcg?cggtttcccg?gacgttggcc?ttggtcaggc 42240
tgtagtcgtt?gagcagcagc?ttcccggcga?tcaccgcctg?cgcgatgggc?gaggtgttca 42300
cggtgagcat?gcccttgagc?ttcgagagct?ggtcggcgta?caggccaccg?cccgccaccc 42360
gctgatccgc?caccgcgtag?ccgacccggg?cgccgggcat?gccggtcttg?gcgaaggatc 42420
cgatgtagac?cacgttcccc?gaccggtcga?gcgctttcag?cgtggggagg?cgctcggcgc 42480
cgaagagccc?gtacgcgttg?tcttccagca?gaaggatccc?gtgggccgcg?gcgacttcga 42540
gcagccggtg?gcgggacggc?agatccatgc?tggtgccggt?ggggttggcg?aagttgggcg 42600
tcacgtagca?ggcccgcacc?cgcatgccct?gttcgtcggc?gcgcttcagc?tggaggacga 42660
gatcctcggg?gtcgatgccg?ttctcgccgg?accggaccgg?ccagaccggc?gtgtcggtga 42720
gcagcgccgc?cccggtcagg?ccgacgtagg?tgggcgccgg?ggcgagcagc?acgtcccgct 42780
cgttcgcccg?cagggtgcgg?agcaccagga?acatggcctc?ctgggcgccg?acggtgacga 42840
ccaccgattc?cggggcggcg?tcgatgttct?cgtcctcggc?caggttgcgg?gcgatgatgt 42900
cggcgatgat?gcccttcgtg?gtgccgtatt?ggaaaagcgc?gcgggtgacg?ccggcctcgt 42960
ccactttccg?atcgcgccgg?agatgctcgc?agtacgcgtc?gagataatcg?tgaatgagcc 43020
ggatgtcgaa?aaactcttcg?tatggccgtc?ctgccgccat?ggaaatggcc?acagggtatt 43080
cgtcgatcag?ttcattgagc?agattcatcg?acgagatggc?cggatcggtg?agcgatccgt 43140
gcagcgtttc?catgtccaaa?tgggtggaca?aaacgttcga?atccataaat?gctaggattt 43200
ccacccggtg?gcgaagtgtc?aagcgggggt?gtggacgcga?ttcggtgccg?attttccgcc 43260
atgtccagtg?ctgattcctt?ccgaataacg?gatctccgaa?ggtacggtca?ccgcagtgcg 43320
ccgcgtagcc?ggggatcgca?cgttccacat?cagacggaaa?ccggtggccc?gggtgattcg 43380
cggccgccat?gtcgctgcgc?gccgtggctg?tgcggtgagc?ggggaaggac?catcatgctg 43440
atgacaaccg?agaacgggat?ccggttgtct?taccacgatc?agggcaacgg?cccggccgtc 43500
ctgttgctga?ccggcaccgg?cgcgccgagt?tccgtctggg?atctgcacca?ggttcccgtg 43560
ctccgcgccg?ccggattccg?cgtgatcacc?atggacaacc?gcgggatccc?gccgagcgac 43620
gagggcacgg?acgggttcac?catcgacgac?ctcgtcgcgg?acgtggcggc?gctgatcgac 43680
tacctcgacg?ccgtgccgtg?ccgcgtgatc?ggcacgtcga?tgggttcgta?cgtcgcgcag 43740
gagctggcac?tcgcccgtcc?ggaactgctg?gactcggtcc?tgttgatggc?ggcctgcggc 43800
aagagcagcc?tcgtccagcg?agaactcgcg?gaaggcgaag?cgaagctgat?cgaacaaggc 43860
atcgagctgc?caccggcctt?cctcgccgcc?gtccgggcca?tgcacaacct?gggacccgcg 43920
accctcgccg?acgacgacct?cgccggcgac?tggctcgacc?tgttcgccgc?ggcgggatcc 43980
tgggggccgg?gtgtgcggga?gcagctgctg?ctgagcgcgt?tgcccgaccg?cctcgaggcc 44040
taccgctcga?tcaaggtgcc?ctgtcacgtc?gtctcgttcg?aacacgacct?cgtggcgccg 44100
ccgtccgccg?gacgcgagct?ggcggacgcg?atccccggcg?cgacccaccg?cacgattccc 44160
gggtgcgggc?atttcggcta?cctggagaac?ccggaggcgg?tgaaccggga?gctgatccgg 44220
ttcctccgca?ccgaatcccg?tgagactttg?ggggagaccg?catgaccgtt?ttttcggcgg 44280
ccacgacacc?ggccctgttc?gaggcgaccg?cagccgtgct?gccggatcgg?ccggcggtgg 44340
cgatggacac?caccacactc?acctacgccg?aactcaacgg?cgaagccaac?cggctcgcgc 44400
gacggctcgt?ggcgcacggc?gtgggcccgg?agcggctggt?cgcgctggcc?atgccgaggt 44460
cgatcgagtt?cgtcatcgcg?atcctggccg?tgcacaaggc?gggcgccgcg?tacgtgccgg 44520
tcgacccgga?ctatccggag?gaacgcaaac?gccacatgct?ggacgacacg?gcggcgcagc 44580
tcgtgctgtg?cctgccgggg?caggaagtgc?ccggtgacgg?cgtcgtcctg?agcgtggaac 44640
gggaaccggg?gctgtccgag?ccgaacctgg?acgacgggga?ccgcctcggc?ccgttgctgc 44700
ccgaccatcc?cgcgtacgtc?atctacacct?cgggctcgac?cgggaaaccg?aagggcgtgc 44760
tggtcacgca?tcgcgggatc?ccgaacctgg?ccgacgacta?cgtgcgccgc?caggaactgg 44820
tgccggacag?caggttgctg?gccttcgcct?cgccgagctt?cgacgccgcg?gtcgccgagt 44880
tctggccgat?ctggcaggcc?ggtggctgtc?tcgtgctggc?ctccgcgccg?gacctggtcc 44940
ctggcgagcc?gctcgggagg?ctggtgcgag?agcaggggat?cacccacatc?acgctgccgc 45000
cgtcggcgct?ggcgccactg?gaggaggcgg?gcggcctgcc?cgccgggctg?accctcctgg 45060
tggcgggcga?ggcgtgcccg?gcgccggtgg?ccaaacgctg?gtccatggac?cgcgtgatga 45120
tcaacgccta?cggccccacc?gaagtcaccg?tggcggtgac?cgcgagcgag?ccgctgtccg 45180
gtgaggagac?cccgccgatc?ggcaggccga?tcaccggtgt?gcgcacctat?gtcctggacg 45240
aactgctccg?gcccgtcccg?gacggggacg?tgggcgagct?gtacacgatg?ggtcccggcc 45300
tcgcccgcgg?atatctcagg?cggcccgccg?cgaccgcgga?gcggttcctg?ccggatccgt 45360
tcggcgaacc?gggcgggcgc?atgtaccgca?ccggcgaccg?ggtgcggcgg?cgtccggacg 45420
gccagttcgt?cttcgtcggc?cgcgtcgacg?accaggtgaa?ggtccgcggt?caccggatcg 45480
aaccgggcga?ggtggagtcc?gccttgctcg?ccgtggacaa?ggtggcccag?gcggtggtga 45540
ccgcgcacga?caaccggctc?gtcgcctacg?tcgtcggcac?cgggggcgaa?cgcgtgacgg 45600
ccgaagagct?cctcccgccc?ctgcgggagc?ggatgccgtc?ctacctggtc?cccgacgtgg 45660
tcgtcagcct?gccgagactg?ccggtctcgc?cgaacggcaa?ggtcgaccgg?accgccctgc 45720
ccaccccgga?agaggagagc?gccgagcgcg?cggcgtcggg?acgggcgccg?agcacaccga 45780
cggaaatcct?tctcgccgaa?ctgttcgccg?acgtgctcgg?tgtcgccagt?gtcggggtcg 45840
aggacagctt?cttcgagatc?ggtggtcatt?cgctgctcgc?gacccgggtg?gtgagccgga 45900
tccgcgaaag?cctgaagatc?cggctgagag?tgcaggcctt?cttcgacgcg?ccgaccgtgg 45960
cacagctcgc?caaggtgctc?gacggcgcac?tcacctgacc?tggagaactt?catgcagacg 46020
acgaaaaccg?tcgacctcgg?cgatcccgat?ctgtacacca?ccctcgaccg?tcacacccgc 46080
tggcgcgagt?acgcggcgga?agacgcgatg?gtgtggagcg?aaccgggcag?ttcacccggc 46140
ggcttctggt?cggtgttctc?gcaccgggcg?tgcgccgcgg?tgctcggccc?gtccgccccg 46200
ttcacctccg?agtacgggat?gatgatcggc?ttcgaccgcg?agcacccgga?caattccggg 46260
ggccagatga?tggtggtgtc?cgaacaggac?cagcaccgca?aactgcggaa?gctggtcgga 46320
ccgctgcttt?cccgggcggc?ggcgcggaaa?ctgtcggacc?gggtgcggac?cgaggtgcgc 46380
ggcgtgctcg?accgggtgct?cgacggcgag?gtgtgcgacg?tcgccgcgga?gatcggcccg 46440
cgtatccccg?ccgcggtcgt?ctgcgagatc?ctcggcgtgc?ccgccgagga?ccaggacatg 46500
ctcatcgacc?tgaccaatca?cgcgttcggc?ggcgaggacg?agctgttcga?cgggatgacg 46560
ccgcgtcagg?cgcacaccga?gatccttgtc?tacttcgacg?aactgatcag?cgctcgccgc 46620
gcgaaccccg?gcgacgacct?cgtcagcacg?ttgctgtccg?acggcgaact?ctcgatcgac 46680
gacgtggtgc?tcaactgcga?caacgtgctg?atcggcggca?acgagaccac?ccggcacgcg 46740
atcaccggcg?cggtgcacgc?tttcgccgcc?gtgccgggac?ttctggaccg?ggtgcgggac 46800
ggcgacgtcg?acgtcgaccc?cgtcgtggac?gaggtgctgc?gctggacctc?gcccgcgatg 46860
cacgtgctac?gcgtgacgac?cggcgaagtc?acgctcaacg?gccgcgacct?cgaagccggt 46920
acgccggtgg?tcgcgtggct?gcccgccgcg?aaccgggatc?ccgccgtgtt?cgaggaaccg 46980
gacacgttcc?ggccggggcg?gaaacccaac?cggcacatcg?ctttcgggca?cggcatgcac 47040
cactgcctcg?gctccgcgct?cgcgcggatc?gagctggcgg?tcgtgctgcg?ggaggtggcc 47100
ggccgggtgt?cacgcgtgga?gctggagaag?gaaccggcct?ggctgcgcgc?gatcgtcgtg 47160
caggggtacc?gggaactccc?ggtgcggttc?accggacgct?gagtcctatg?tggacgtaag 47220
tgtcaagcgg?acaccgttga?tgcacaaagg?gatgcgggcc?atcctgtgat?cgagggttac 47280
atctattcca?cggaggaatg?ccttgtctgc?tcaagaaagc?acgcagaatt?tcgagatcga 47340
ctatgtggaa?atgtacgtcg?ccaacctcga?ggcggccgcg?tccggctgga?tggacaagta 47400
cggcttctcc?gtcaccgcca?ccgaccggtc?ggccgaccac?cggagcgtga?cgctgcggca 47460
cagcgcgatc?gcgctggtgc?tgaccgagcc?gctgtcggac?cggcaccccg?gggcgaccta 47520
cctgcagacc?cacggtgacg?gggtcgccga?catcgcgctg?cgcacccccg?acgtggccgc 47580
cgctttcgag?gccgcggtga?aggcgggcgc?ggaacccgtc?cgcgagccgg?agaagcgggc 47640
ggactcggtc?gtcacggcca?ccgtcagagg?gttcggcgat?gtcgtgcaca?ccctgatcca 47700
gagcgacgcc?gccgaggaga?ccccgcgcgg?caagggcggc?gtggagctgg?gcgtgatcga 47760
ccacttcgcg?gtctgcctca?acgccggcga?cctcggcccc?acggtggcgt?tctacgagcg 47820
cgcgctcggt?ttcaagcaga?tcttcgagga?gcacatcgtg?gtcggcgcgc?aggcgatgaa 47880
ctccaccgtc?gtgcagagca?cgtcgggcgc?ggtcaccctc?accctgatcg?agccggacaa 47940
gaccgccgac?cccggccaga?tcgacgactt?catcaaggag?caccacgggt?ccggcgtcca 48000
gcacatcgcc?ttcaccagcc?cggacgcggt?ccgcgcggtc?aaggagcttt?ccgcgcgcgg 48060
cgtggaattc?ctgaagaccc?cggacgccta?ctacgacctg?ctcggcgagc?ggatcgagct 48120
ggagacgcac?tcgctggacg?atctgcgcga?gacgaagctg?ctcgccgacg?aggaccacgg 48180
cggccagctg?ttccagatct?tcaccgcctc?cacgcatccg?cgtaagacga?tcttcttcga 48240
gatcatcgag?cgccagggcg?ccggcacgtt?cggcagctcg?aacatcaagg?ccctgtacga 48300
agccgtggag?ctggagcgca?ccgggcagag?caagctcggc?cccgtccgtc?gatgacccac 48360
gtctgtctcg?acgacctcga?gcgcgcggca?cgcgatgtcc?tctccggcga?gaactgggat 48420
ttcctcgccg?ggggcagcgg?cgcggaagcc?tccttggagg?ccaaccgcgc?cgcgctcgag 48480
cggatcttcg?tgatcccccg?ggtattgcgt?gagctgaccg?gtggcaccgt?cgaggccgaa 48540
ctcctcggcg?gtcgcgccgc?gctgccgctg?gcggtcgcgc?ccgtcgcgta?ccagcggttg 48600
ttccaccccg?aaggcgagct?cgccgcggca?cgcgccgccc?gggacgcggg?cgtgccgtac 48660
accatctgca?cgctgagcag?tgtcccgctg?gaggagatcg?cggccgtcgg?cggacggccg 48720
tggttccagc?tgtactggct?gcgggacgag?aaacgctcgc?tcgacctcgt?ccgccgcgcg 48780
gaggacgccg?gctgcgaggc?gatcgtgttc?accgtcgacg?tgccgtggat?gggacgacgg 48840
ctgcgggaca?tgcggaacgg?cttcgcgctg?ccggattccg?tgaccgcggc?caatttcgac 48900
gccggggcgg?ccgcgcacca?ccgcactcgc?ggccggtcgg?cggtggcgga?gcacaccgcg 48960
cgcgaattcg?ccccggccac?ttgggaatcc?gtcgaagcgg?tccgtgcgca?cacggatctc 49020
ccggtggtgc?tcaaaggaat?cctcgcggtc?gaggacgcga?gccgcgccgt?cgacgcgggc 49080
gccgccggga?tcgtggtgtc?caaccacgga?ggcaggcagt?tggacggcgc?cgtgccgggg 49140
atcgagatgc?tgggcgagat?cgccgccacc?ctttccggct?ggccgggcga?actgctgctc 49200
gacggcggga?tccgcggtgg?cggcgacatc?ctcaaggcga?tcgcgctggg?ggcgtcgggt 49260
gtcctggtcg?gacggcccgc?catgtggggg?ctcgccgcgg?gcggggagga?cggcgtccgg 49320
caggtgctcg?acctgctcgc?cgtcgaattc?cggaacgcgc?tgggcctcgc?gggctgcgac 49380
tcggtggacg?cggcccggcg?gctgggcacg?aaggtctccc?gccgcggctg?acgccgttgc 49440
cgttcttacc?cgaaggagga?aggtgcttct?ctcgctcccg?gcgccggtcg?ccccgatcgc 49500
cgcccacagt?ctcctgctgt?tcttgctgca?ggtcggttcg?ctgctcctgc?tcgccgtcgt 49560
cttcgggcgg?ctggccgccc?ggttcgggat?gcccgccgtg?gtcggcgaac?tgctggtcgg 49620
ggtgcttctc?ggcccgtcgt?tgctggcgtg?ggccgcaccg?gggctgcacg?ggtggctttt 49680
ccccgccgtc?accgagcagt?accaccttct?cgacgccgtc?gggcagatcg?gtgtcatcct 49740
gctggtcggt?ttcaccggta?tgcagatgga?catgggcctg?ctccggcggc?ggcgtgccgc 49800
ggcggccgga?gtgggcctcg?gcggcctcat?cgtcccgctc?ggactgggca?tcggcgcggg 49860
ttacgtactg?cccaagatgc?tgatccccga?aggcacggac?agcaccgtct?tcgcgctttt 49920
cctcggcgtg?gcgttgtgcg?tcagcgccat?cccggtcatc?gccaagacgc?tgatggacat 49980
gaaactgctg?caccgcaacg?tcgggcagct?cacgctggcg?gccggcgcga?tcgaagacgc 50040
cttcggctgg?ttcatgctgt?cggtcgtcag?cgcgatggcg?gtcaccgccg?tgtccgcggg 50100
tgccgtgctg?atctcgctcg?gctatctggt?cggcgtcgtc?gtcttcgccc?tcaccatcgg 50160
caggccgctg?gtccggggcg?tcatgcgcac?cgcggcccga?tccgacggtg?cggggctcac 50220
cgtctccgcc?gccgtcatcc?tgatcttcct?ggcctcggcc?gggacgcagg?ccctcggtct 50280
ggaggcgatc?ttcggtgcct?tcctctgcgg?catcctgatc?aacaccgcgg?gcaaggtgga 50340
tcccgtcaaa?ctggctccgc?tgcgcaccgt?cgtccttgcg?gtcttcgcgc?cgatcttctt 50400
cgccacggcc?ggattgcgga?tggatctcac?cgcgctgatc?cgcccgcagg?tcctgctcac 50460
cgggctcgcg?gtgctcgcgc?tggccatcat?cggcaagttc?gtcggcgcgt?acgccggcgc 50520
ccggatcagc?ggcttgaaca?aatgggaggg?gctcgcgctc?ggcgcgggac?tgaacgcgcg 50580
gggtgtcatc?gagatcgtgg?tcgccatggt?ggggctgcgg?ctgggcattc?tcagcgtcga 50640
gatctacacg?atcatcatcc?tcgtcgcgat?cgtcacttcg?ctgatgacgc?cgccgatcct 50700
gcggttcgcg?atgaagcggg?tggaacagac?cgccgaggag?gaggtgcggg?agaccgagca 50760
ccgggcttgg?gccgcggaac?cggtgaagaa?cgagcaggaa?cattcgctct?agcgccccgc 50820
tgctctcggg?cttaaggctc?ccttcaccgc?gtctgatgcg?acgaaaggag?ccttcaccac 50880
agccgccggc?cgaacccggc?ccgcgacggg?attggacgat?ctggaccgtt?ccgccggacc 50940
gaagttacct?ttccgtcagg?gcgcggagcg?taggaagcga?aatttccgtt?ggcacgcaaa 51000
ggggatagac?ggccatgaca?aggagtcctt?cgacgctggt?gcgcccccgc?gacggccagg 51060
atcacgcgga?ccgtgtcgcc?ttgtccgccg?cgaccaccga?tggcgtgcac?atgcggaccg 51120
ccgacgtccg?cgcgtggatc?gccgaacgcc?gtgaggccaa?cgtcttccac?gtcgaacgca 51180
ttccgttcgc?cgcgctggac?caatggggtt?tcgaggacgg?gaccggcaac?atcgcgcacc 51240
ggagcggacg?gttcttcacc?atcgaagggc?tgcacgtggt?cgaggccgac?ggcccgcacg 51300
gcgacggtcc?ctaccgcgaa?tggcagcagc?ccgtcatcaa?acaggccgaa?gtcggcatcc 51360
tcggcatcct?ggccaaggag?ttcgacggcg?tcctgcattt?cctgatgcag?gccaagatgg 51420
agccggggaa?tcccaatctg?gtccagctgt?ctccgaccgt?gcaggccacg?cgcagcaatt 51480
acaccaaggc?gcacggcggc?acgaacgtca?agctgatcga?gtacttcgcg?ccgcccgacc 51540
ccgagcgggt?catcgtcgac?gtgcttcagg?cggagcaggg?atcgtggttc?ctccagaagt 51600
ccaatcgcaa?catgatcgtc?gagaccgccg?acgaagtgcc?gctgtgggac?gacttctgct 51660
ggctcaccct?cgggcagatc?gcggagctga?tgcacgagga?cgagacgatc?aacatgaacg 51720
ccaggagcgt?gctgtcgtgc?ctgccgtacc?acgacaaggc?tcccggcgcg?atgttctccg 51780
acgtccagct?cctgtcgtgg?ttcacgaacg?agcggtcgcg?gcacgacgtg?cgcgcccgcc 51840
gcatcccgct?caaggacgtc?cgcgactgga?agcggggtgc?cgaggcgatc?gagcacgagg 51900
acggccgcta?cttcagggtc?ctcgcggtcg?cggtgaaagg?cagcaaccgc?gagaaggtca 51960
cctggaccca?gccgttgatc?gaatccgtcg?ggatgggcct?cgtcgcgttc?ctcatgcgtg 52020
acttcggcgg?cgttccgcac?gtcctggcgc?acgcccgcgc?cgacggcggg?ttcctggaca 52080
cggtcgagct?ggcgccgacc?gtccagtgca?cgccgcagaa?ctacctgcat?ctgcccgccg 52140
agcaacggcc?gccctttctc?gacctggtcc?tcggcgcccc?gcgatcacgg?atccgttacg 52200
aggcggtaca?ttccgaagaa?ggcggccgct?tcctcagcgt?ccaggcgcgt?tatctggcga 52260
tcgaggcgga?cgacacggtc?gagcctccgc?ccggctacgc?ctggatcact?ccggggcagc 52320
tcaccgcact?cacccggcac?gggcactacg?tcaacgtcga?ggcccgtacg?ctgctcgcct 52380
gcctcaacgc?cgcggccgcg?cagccacggg?gcggtgcctg?aatgaagacg?atcaccgtcc 52440
tcggcgcgtc?gggtttcgtc?ggctccgccg?tcgtgcgcgc?gctggcgcgg?cggccggtcc 52500
gggtgcgggc?ggtggcgcgc?aggcggctca?cgcccccgcc?cggccgggcc?gagatcaccg 52560
ccgtcgccgc?cgatctcacc?gaccgcgccg?cgctcgccgg?cgcggtcgcc?ggctcggacg 52620
cgatcgtgca?cctgctgctc?ggtgacggcg?gctggcgtgg?cgcggagggc?gccgaacgcg 52680
tgaacgtggg?agtcctccgg?gacctcgtcg?agatcgccgg?cggtggcgac?gggccgcctc 52740
cggtggtggt?gttcgccgga?accaccgcgc?aggtcggggt?gccgccgcgg?gaaccgctcg 52800
acgggaccga?accggaccac?ccggcgactc?cttacgacgc?ccagaaactc?gaagcggaac 52860
aactcctcaa?gaaggccacc?gccgacggcc?tggtgcgcgg?gatcagcctg?cggctgccga 52920
cgatcttcgg?cgagaccgcg?gccgagggcg?cgaaccacga?ccgcggcgtg?gtgtccacca 52980
tggcgcggcg?ggcgctcgaa?ggactaccgc?tcaccgtctg?gggcgacggc?acggtgcgac 53040
gcgacctggt?gcacgtcgac?gacgtcgcct?cggcgttcat?ggccgcactg?gaccatccgg 53100
attccctggt?gggcgggcac?tggctgatcg?gtgccggtcg?aggggaccgg?ctgggcgagg 53160
tcttccggct?cgtcgcgaag?gagatgtccg?agcacacggg?gaaaagcccg?gtggaagtgt 53220
cctgtgtgga?gcctccggcg?cacgcgcccg?agacggatct?ccggagcatc?accatcgatt 53280
ccacgccgtt?ccgtgcggtc?accggctggc?gcccggagat?ctcgctgccc?gagggagtgc 53340
gccgcaccgt?cgccgctttg?acgtcatcag?ttcagggaag?ggttcacaca?tgaccacgcg 53400
cgtatgggac?tacctcgccg?aatacggtgc?cgagcgggcc?gatctgctcg?acgcggtcga 53460
gacggtcttc?aactccgggc?agctcgtgct?cggcgcgagc?ctgcgcggct?tcgagtcgga 53520
attcgccgcg?taccacggcg?tcgggcactg?cgccggtctc?gacaacggga?cgaacgcgat 53580
caagctcgcc?ctgcaggcgc?tgggtgtcgg?gccgggcgac?gaggtgatca?ccgtgtccaa 53640
caccgcggca?ccgaccgtgg?tcgccatcga?cgggaccggg?gccaccccgg?tcttcgtcga 53700
cgtccgcgag?gaagacttcc?tgatggacac?cggcctggtc?gaggccgcga?tcaccgaacg 53760
caccaaatgc?ctgctgcccg?tgcacctgta?cggccagtgc?gtggacatgg?ccccgctgaa 53820
ggcgctcgcc?gcgaagcacg?gactgtccct?tttggaggat?tgcgcccagg?cgcacggagc 53880
ccggcagaac?gggacgatcg?cggggacgtt?cggcgacgcg?gccgcgttct?ccttctatcc 53940
caccaaggtt?ctcggcgcgt?acggcgacgg?cggcgcggcc?atcacctccg?acgaagcggt 54000
ggaccggcgg?ctgcggcggt?tgcgctacta?cgggatggaa?gagcgttact?acacggtgga 54060
aacgcccgcc?cacaacagca?ggctggacga?ggtgcaggcg?gagatcctgc?ggcgcaagct 54120
caagcggctc?gacgcgtaca?cggcgggccg?ccgggccgtc?gccgaacgct?atgtcgaggg 54180
cctcggcgac?accgacctga?agcttccgaa?gaccacgccc?ggcaacgagc?acgtgtacta 54240
cgtgtacgtg?gtgcggcacc?cgcgacgcga?cgagatcatc?gagcggctca?aggcgtacga 54300
catccacctg?aacatcagct?acccgtggcc?ggtgcacacc?atgaccgggt?tcgcccacct 54360
cggctacgag?aagggctccc?tgccggtcac?cgagaagctg?gccaccgaga?tcttctcgct 54420
gccgatgtat?cccgcgcttt?cccccgatct?gcaggacaag?gtcgtccacg?ccgtccgcga 54480
agtgctgtcc?agcctctgat?cacgccgacc?acgggagtac?ctgtgcaagc?acgcgaactg 54540
gccgtcgaag?gcgcgatcga?gttcaccccc?aaggtcttcc?ccgacgatcg?cggcctgttc 54600
ctctcgccat?tccaggaaga?ggcgttcacc?gaagcgcacg?gcggcccgct?cttccggacg 54660
gtgcagacga?atcacagcag?ctccaaacgc?ggagtggtgc?ggggcatcca?ctacaccacg 54720
acgccgccgg?gcaccgcgaa?gtacgtctac?tgcgcgagcg?gtcaggcgct?ggacatcgtg 54780
gtcgacctcc?gggtcggctc?gccgacgttc?gggaaatggg?acgcggtcct?gctggaccag 54840
cgtgaccacc?gcgcgatgta?cttccccgtc?ggcgtcggcc?acgccttcgt?ggcgctcgaa 54900
gacgacaccg?tcatgtggta?cctgctctcc?acggcctacg?accccgggaa?cgaactcgcc 54960
ctctcggtcc?tggatcccgc?gctcggcctg?cccgtcgacc?ccggcgtcga?accgatcctg 55020
tccgaacgcg?accaggtggc?gatcacgctc?gccgaggcgg?aacgacaggg?actgctgccg 55080
gactacgcca?cctgcgtgga?gatcgaacgg?cgactgtccg?aagtgcccca?gaccacctga 55140
aagcccactg?tccactccag?accagagaaa?ggtgagatcg?atgcgcgctg?cgcggttcaa 55200
gcagctgctc?cggagcaaat?tgagaacatg?gggatggatg?tatcgatgac?tgccctagcc 55260
gaaaccaccg?cggaccttgc?cgtccacaat?ggattgaccg?agatcacccg?attcgccggc 55320
gtgggcaccg?cggtgtccga?gtcgtcctac?tcgcagtccg?agctgctcga?aatcctcgac 55380
atcgaggatc?cgaagatccg?ctcggtcttc?ctgaacagcg?ccatcgatcg?gcgtttcctc 55440
accctgccgc?cggagggtcc?cggcggagcc?cgcgcggccg?agccgcaggg?cgacctcctc 55500
gacaagcaca?agaagatcgc?cgtcgacatg?ggttgccgtg?ccctggaagc?ctgcctgaag 55560
tcggcgggag?cgacgctttc?ggatctgcgt?cacctgtgct?gtgtcacctc?gaccgggttc 55620
ctgacgcccg?gcctgagcgc?gctcgtcatc?cgggaaatgg?gcatcgaccc?gcattgcagc 55680
cgctcggaca?tcgtgggcat?gggctgcaac?gccggtctca?acgcgctcaa?cgtggtcgcg 55740
ggctggtccg?cggcgcatcc?cggcgaactc?ggcgtcgtcc?tgtgcagcga?ggcgtgttcc 55800
gccgcctacg?cgctggacgg?cacgatgcgg?accgcggtgg?tcaacagtct?tttcggcgac 55860
ggatccgcgg?cgctcgcggt?cgtctccggc?gacgggcgcg?tgccgggtcc?gcgcgtcctg 55920
aaattcgcca?gctacatcat?caccgacgcg?gtcgacgcga?tgcgctacga?ctgggaccgt 55980
gaccaggacc?ggttcagttt?cttcctcgac?ccgcagatcc?cctacgtggt?cggcgcgcac 56040
gccgaaatcg?tcatcgaccg?gcttctgtcc?gggacgggcc?tgcgccgcag?cgacatcggc 56100
cactggctgg?tgcattcggg?cgggaagaag?gtgatcgacg?ccgtcgtcgt?caacctcggc 56160
ctgagccggt?acgacgtgcg?ccacaccacc?ggtgtgctcc?gcgactacgg?gaacctctcc 56220
agcggctcct?tcctcttctc?ctacgagcgg?ctcgccgacg?aagacgtcgc?ccgcccgggg 56280
gactacggcg?tgctcatgac?catggggccc?ggctccacga?tcgaaatggc?gcttatccaa 56340
tggtgacgag?taacgaaagc?aagggcgagc?cgatgctgcg?cttcgacgga?tcccgtccgc 56400
tgtccgccgc?gatcgtcgag?gagatcgacg?cgctgtgcga?tcgagccgag?gacgagcgtg 56460
aacccggacc?agtcaccatc?cacgtcacgg?gcgccccgcc?cgagggctgg?gcgaaggggc 56520
tggcggtcgg?gctcgtgtcc?aaatgggagc?gggtcgtgcg?ccggttcgaa?cgcctcggca 56580
ggctcacggc?cgcggtggcg?tcgggcgaat?gcgccggaac?ggccctggac?ctccttctcg 56640
ccgccgacat?ccggatcgcc?gaaccgggca?ccacgctgcg?gctcgcgtcg?gccggcggtg 56700
gcacctggcc?gggcatgacc?gtgtaccggc?tcaccaagca?ggccggagcg?gcgggcatcc 56760
gccgggccgt?actgctcggg?gcgccactcg?ggacggagcg?cgcgctcgcc?ctcgacctga 56820
tcgacgaggt?gtccgaagac?ccggcgaaga?cgctggccga?gctgaacgtc?gtcatggacg 56880
gcgccgagac?ggcgatccgg?cggcagctga?tcttcgaagc?gggctcgacc?accttcgagg 56940
aggcgctcgg?ttcccacctg?gccgccgcgg?accgggccct?gcggcgggaa?gccaagtcgt 57000
gacgacggat?caaaccgcac?tttcgccagg?gctcgacctc?cgggccctgg?cgggggaggc 57060
ccaccgggtc?gacgacgacg?tccggacgct?gcgtgcggcc?ttcgtcgagg?cgcacgccga 57120
ggagatctac?gccgaactca?ccggcggccg?gacgcggtac?ctgcggatgg?acgaactcgt 57180
ccgcgccgcg?gcgctcgcct?ttccgggtct?ggtgccgtcg?gaggagcaga?tggcggccga 57240
gcgtgcacgg?ccgcaggcgg?agaaggaggg?gcgcgagatc?gaccagggca?tcttcctgcg 57300
cgggatcctg?cgcgcgccga?aggcgggccc?gcatctgctc?gacgccatgc?tccggcccac 57360
cgccagggcg?cagcggctgc?tcccggagtt?ccttgaaacc?ggcgtcgtgc?agatggaggc 57420
cgtccggctg?gagcgccggg?acggcgtcgc?gcacctgacc?ctgtgccggg?acgactgcct 57480
gaacgccgag?gacgcccagc?aggtcgacga?catggagacc?gcggtcgacc?tggtgctgct 57540
cgacccgtcc?gtccgggtgg?gactggtgcg?gggcggggtg?atgagccacc?cccgttacct 57600
ggggcgccgg?gtgttctgcg?cggggatcaa?cctcaagaag?ctcagctccg?gcgacatccc 57660
gctggtggat?ttcctgctgc?ggcgggaaac?cgggtacatc?cagaagatct?tccacggtct 57720
gctgacggag?aattcctggc?attcgaggtt?caccggcaaa?ccctgggtgg?cggccgtcga 57780
ttcgttcgcc?atcggcggcg?ggacccagtt?gctgctggtc?ttcgaccacg?tgctggcggc 57840
ctccgacgcg?tacctcagcc?tgcccgcggc?caaggagggg?atcatccccg?gcgtctccaa 57900
cttccggctt?tcccggatcg?ccgggccgcg?ggtggcccgg?caggtgatcc?tcggcggccg 57960
caagctccgg?gcggacgagc?cggacgcccg?ctcgatcgtc?gacgaggtcg?tgcggccgga 58020
ggaggtggac?tcggccatcg?accgcgcgct?cgcccggctc?gacggggaag?ccgtcgcggc 58080
caaccggcgc?atgctgaacc?tttccgagga?accaccggag?gaattccgcc?ggtatatcgc 58140
cgaattcgcg?ctgcagcagg?ccctgcgcat?ctacggggcg?gacgtgatcg?gcaaggtgga 58200
cggcttcgcg?gtggggtcgc?gatgaacggc?gtccggtacg?agaagaagga?ccacgtcgcc 58260
tacgtgacga?tggaccggcc?cgaggtgctg?aacgcgatgg?accggcggat?gcacgcggaa 58320
ctcgccggga?tctgggacga?cgtcgaagcc?gacgacgacg?tcagggcggt?ggtcctgacc 58380
ggggcggggg?atcgggcgtt?ctccgtcggg?caggacctca?aggaacgcgc?gcggctgacc 58440
gaggaaggcg?tcgaggcgtc?gacgttcggc?agcgccggtc?agccgggcca?tccccggctg 58500
accgaccggt?tcacgctgtc?caaaccggtg?gtcgcgcgtg?tgcacggcta?cgcgctgggc 58560
ggcggcttcg?aactggtgct?ggcgtgcgac?atcgtcgtcg?cttccgaaga?agcggtcttc 58620
gggttgccgg?aggtccgtct?cgggctgatc?gccggggcgg?gcggcgtttt?ccggttgccg 58680
cgccagcttc?cgcagaaagt?ggcgatgggt?catctgctga?ccgggcgcag?aatggacgcg 58740
gcgacggcac?tgcgtcacgg?cctggtcaac?gaggtcgtcc?cggtcgcgga?attggaccgg 58800
tgcgtcgccg?aatggacgga?cgatctggtg?cgtgccgcgc?cgctttcggt?acgggcgatc 58860
aaagaggccg?cgctgaagtc?actcgacctc?ccgctcgaag?aagcgttcaa?aacctcgtat 58920
ccgtgggaag?aacggcggcg?gacgagcgcg?gacgcgagtg?agggcgcccg?cgccttcgcc?58980
gagaaacggg?acccgatctg?gaccggtcgc?tgaatccggg?ttggaccgtt?gt 59032
<210>2
<211>849
<212>DNA
＜213〉east amycolatosis
<400>2
gtgacggtga?ccatcgagaa?ggcgcttgtc?gtcggtacgg?gactgatcgg?cacctccgtc 60
gcgctggccc?ttcgcgaacg?gggcgtggcg?gtgtatctgt?ccgatgtaga?cgctcgggcc 120
gtccggctgg?cgggggacct?cggcgccggc?cgggaatgga?ccggtgagga?agccgacctg 180
gcgctgatcg?ccgtcccgcc?gcatctcgtg?ggtgagcgac?tggcggacct?gcagcggcga 240
ggagccgcgc?gggtgtacac?cgatgtggcg?agcgtcaagg?tcgacccgat?cgccgacgcg 300
gaacggctgg?gctgcgacct?gagttcctat?gtgccgggcc?atccgctcgc?cggccgggaa 360
cggtccggcc?cgtccgccgc?ccgctccgat?ctgttcctcg?gccgtccgtg?ggcgatctgc 420
cccggcgccg?agacggccgc?ggatgccgtc?cggctgacgc?gggaactggt?ttcgtcgtgc 480
ggagcgaggg?cggtcacggt?ggaggcggcc?gagcacgatg?cggcggtggc?gctggtgtcg 540
cacgctccgc?atgtggtggc?gtcggcgatg?gcggcgagtc?tcgcgggcgg?cgacgacgtc 600
gcgctgggcc?tgtcggggca?gggactccgc?gatgtgacgc?ggatcgcggc?cggggatccc 660
ttgctgtggc?gaaggattct?cgccgggaac?gcccagccgg?tcgccggggt?gctcgaccgg 720
atcgcgggcg?acctcgcggc?ggcggcctcg?gcgttgcggt?ccggcgatct?ggacgaggtg 780
acggatctgc?tgcggcgcgg?ggtggccggc?catggccgga?tccccggccc?gcgcggcgcc 840
aaagcaggc 849
<210>3
<211>9459
<212>DNA
＜213〉east amycolatosis
<400>3
atgacgatga?attccgcagc?gcggaccaca?cggacggtgc?tggagctgtt?cgcgtcgcac 60
gtggaccgga?cgcccggcgc?ggtggccgtg?gtcgacggtg?accgggtgct?gacataccgg 120
cagctcgacg?agcaagcgag?ccggttggcc?ggatgcctgg?caggccgggg?aatccgccgt 180
ggcgaccggg?tcgcggtgat?catgggccgg?tcggcggacc?tggtggcggc?gctgctcggc 240
gtgtggaagg?cgggggcggc?gtatgtgccg?gtcgacgccg?cctatcccgc?tccgcgcgtg 300
gaattcatgg?tgtcggactc?ggcggccgcg?ctgacggtgt?gctcggccgc?cacccgttcc 360
gccgtaccgg?cgggggtggc?gtcgctcgaa?gcggacgccg?tcacggacga?ggacgcgggc 420
ggcactccgg?tcgccgcggc?gcgcccgggg?gatccggcgt?tcgtgatgta?cacgtccggc 480
tctacgggca?cgccgaaggg?agtggttatc?tcccagagcc?gtgtcgcgga?gctggtgcag 540
gaccccggtt?gggagatggg?gccggacgac?gcggtgctca?tgcactcgcc?gcacgccttc 600
gacgcgtccc?tgctcgaaat?ctggacgccg?ctgtcctcgg?gggcccgggt?ggtgatcgcc 660
gaaccgggcg?tgatggacgc?ccggcgcctg?cgggaagtgg?ccgccgcggg?agtgacgcgc 720
gcgtacctga?ccgcggggag?tttccgtgcg?gtggctgagg?aatcgccgga?atccttcgcc 780
gggttccgcg?aggtgctgac?cggcggcgac?gtggtgcccg?tgcacgcggt?ggagcgcgtg 840
cggcaggcct?gcccccgggc?gcgggtccgg?catatgtacg?gaccgaccga?agcgacgatg 900
tgcgcgacgt?ggcatctgct?ggagccgggt?gacgtgctgg?ggacggtgct?gccgatcggg 960
cgtccgctgg?cgggccgcgc?ggtccacgtg?ctcgacgagt?cgctgcggcc?cgtcgggccg?1020
ggtgtggtcg?gcgacctgta?tctctcggga?agcctggcgg?agggctactt?cggcaaggcc?1080
gggctgacgg?cggaacggtt?cgtggcggat?ccctccacgc?cgggacagcg?gatgtactgg?1140
acgggcgacc?tcgcccagtg?gaccgccgag?ggcgagctgc?tgttcgcggg?ccgggccgac 1200
aaccaggtga?aggtccgcgg?gttccggatc?gaacccggcg?agatcgaggc?cgcgctcatc 1260
gcccagccgg?acgtccagga?cgccgtggtg?gcggcgatcg?acggacggct?catcggttat 1320
gtggtggcgg?gtgaggacgt?cgaccccgcg?ctcgtccgcg?agcgtctccg?tgcggtgctg 1380
ccggagtacc?tggtcccggt?cgccgtgctc?gtgctggacg?cgttgccgct?gaccggcaac 1440
ggcaaggtgg?accgggcggc?gctgcccgca?cccgagttca?cggcgagcgc?cacgagccgg 1500
gagcccgcca?ccgaggccga?gcgtgtcctt?tgtggactgt?tcgccgaggt?gctccggatc 1560
gaccgggccg?gtgtcgacga?cggattcttc?gaactgggcg?gggattcgat?cctcgcgatg 1620
cgcctggcgg?cgcgcgcggc?caaggccggt?ctgctgctga?cgccggtcca?gatcttcgag 1680
gagaccactc?ccgcgcggct?ggccgccgtg?gcgcgggacc?tgcccgccga?ccggcccgcc 1740
gacggtgccc?tgctcagcct?gaccgaggcg?gaagaggcgg?agctggcgct?cgccgctccc 1800
ggcgccgacg?agatctggcc?gctggccccg?ctgcaggagg?ggctgctctt?cgaatcgatc 1860
ctcgacgacc?agggccccga?catctaccag?gtgcaggtga?tcctggagct?gaccggcgag 1920
gtggacgaga?cccggctgcg?agccgcgtgg?gacaccgtcg?tccggcggca?cgacgagctg 1980
cggctgacct?tccaccggct?cgaatcgggc?aagaccgtgc?aggccgtcca?cggggatgtc 2040
accgtgccgt?ggcggacggt?ggatctgacc?ggcgccgacg?acgtcgacac?cgccttcgaa 2100
gccctggcga?tgcaggagca?ggcgcatcgg?ttcgacctcg?ccacggcgcc?gctgatccgg 2160
ctggtgctcg?tccgggtcgg?ggaggaccgg?caccggctgc?tggtcgtcat?ccaccacatc 2220
ctggtcgacg?gctggtcggt?ggcggtcatc?ctcaacgagg?cgttcgaggc?ctacgagccc 2280
ggcgggcggc?tgccggaacg?gcgggagggc?gccggctatc?tcgactacct?ggcttggctg 2340
gagaggcagg?acaaggacgc?ggcacgcgag?gcctggcggg?cggagctgtc?cgacctcgac 2400
gagcccgcgc?tcatcgcgtc?ggcgtcggcc?gcgacggact?acgactaccg?cgtcacccac 2460
ctgccgcccg?cgctgcacga?cggcctcctg?gaattcaccc?gtgagcacgg?gctgacaccg 2520
agcacggtgg?tgcacgccgc?ctgggcgatg?gtgctgtcgc?ggctcacgcg?gcggaccgac 2580
gtggtgttcg?gcaccatggt?cgcgacccgc?cccgcggaac?tgccgggcat?cgagtcgatg 2640
ccgggcctgc?tgatgagcgc?gatgccggtc?cgtgtgacgc?tggacggcgg?gcaggcggtc 2700
atggacatgc?tcaccgggct?gtacggccgt?cagctggccc?tcatgccgca?tcagcacctg 2760
ggactgccgg?agatccagaa?ggcggccgga?cccggcgcga?ccttcgacac?gctgctggtg 2820
gtcgagaact?acccgcggga?atacgccggc?cggtacaagt?acctgcgcac?gatcgaaggg 2880
acccactatc?cgctgacgct?cggcatcacc?ccgggggacc?ggttcaagat?ccagctcggc 2940
tactggcagg?gccaggtccc?ggacgccgtc?gccgactcgc?tgctggagtg?gttcgtcggt 3000
gccatcggcg?ccctggtcgc?cgatcccgcc?ggtccggtgg?ggcggatcgg?ggtgggcacg 3060
gcggaggcac?gcgactggcg?aacgccactc?gtggcggggg?aaccgctgca?cacgctggtg 3120
gggcggatgg?ccgccgagcg?gccggacggc?gtggcgctgg?tggacggcga?cggcgagctg 3180
acctacgcgc?ggttgtggga?tctctcggtg?cggttcgccg?ccgtgctgcg?cgcccacggc 3240
gtcggggcgg?agtcccgggt?cggcctggtg?gtgggacggt?cgtcctggtg?gaccgtcggc 3300
atgctgggcg?tcctgctcgc?gggcggcacg?ttcgtgccgg?tggacccggc?gtatccggac 3360
gagcgcaagg?aaacgatctt?ccgcagcgcg?gatccgatgc?tggtggtgtg?cgcggcgtcg 3420
acccggggga?ccgtgcccgc?cgagtacgcg?gaccggtcgc?tggtgatcga?cgaggtcgag 3480
ctgacggcgg?cgacggagga?agagctgccg?cgggtggatc?cgcgtagtgc?cgcgtacgtg 3540
atctacacgt?cgggatcgac?ggggaccccg?aagggcgtcg?tcgtcaccca?caccgggctg 3600
gggaacctgg?cgctggcgca?tattgaacgg?ttcgccgtga?agccgtcgtc?gcgggttttg 3660
cagttcgcgg?cgctcggctt?cgacaccatc?gtgtccgagg?tgatgatggc?gctgctgtcg 3720
ggagccacgc?tggtggtgcc?gccggagcgg?gagctgccgc?cgcggatggc?gatgtccgaa 3780
acgctggcac?ggtgggacat?cacgcaggtg?aaggcgccgc?cgtcggtgct?gggcacggtc 3840
gacgtgctgc?cggaccgggt?ggacaccgtg?gtcgcggcgg?gcgaactctg?cccgcccggc 3900
ctggtggacc?ggatgtccgc?gaaccaccgg?atgatcaacg?cctacgggcc?gaccgagacc 3960
acgatctgcg?cgacgatgag?catgccgttg?tcgccgggcc?agcacccgat?cccgttcggc 4020
ggaccggttc?cgggagtgcg?cgcctatctg?ctggactcgt?tcctgcgccc?gttgccgccc 4080
ggcatcaccg?gtgagctgta?tctggcgggc?atcggggtgg?cgcgcggtta?cctgggccgt 4140
ccggcgctga?cggccgagcg?gttcgtcgcc?gatccgttcg?cccccggtga?gcggatgtac 4200
cggaccgggg?acctggcgta?ctggacggat?cagggcgaac?tggtgtccgc?cgggcgggcc 4260
gatgaccagg?tgaagatccg?cggcttccgg?gtcgaaccgc?gcgaaatcga?gttcgcgctg 4320
tccgcgtacc?cggggatcac?ccaggccacc?gtgaccgtcc?gcgaagaccg?cctgatcgcc 4380
tacgtgacgc?cgggagacat?cgacacccag?gcggtgcggg?cgcagctcgc?gtcgcggatg 4440
ccccagtaca?tggtgcccgc?ggcggtggtg?gcgctggacg?tcctgccgct?gacggcgcac 4500
gggaagatcg?accggcgcgc?gttgcccgac?cccgatttcg?tcaccgggac?gcccgccagg 4560
gagcccgcca?ccgagaccga?gcgggtgctg?tgcgagctgt?tcgccggcgt?gctcgggctg 4620
gagcgggccg?gggtggacga?cagcttcttc?gacctcggcg?gggactccat?cctctcgatg 4680
cagctcgcgg?cgcgggcgcg?gcgttcggga?ctgagtttca?ccgcggcgga?cgtcttcgac 4740
gggaagaccc?cggagcgcat?cgcgcagctg?gcggaggtgt?cttcgccgcc?ggaacaggcc 4800
gcttccccga?agcccgacgg?tgtcggcgac?gtcgcctgga?cgccggtgat?gtcgctgctg 4860
ggcgacggcg?tcacgggacc?cggattcgcg?cagtggatgg?tggtcgcgac?acctccgggc 4920
ctgacggaaa?aggctttggc?ggcgggattc?tcggccgtgg?tggcgacgca?cgacatgctg 4980
cgggcccgcg?tcgtcgaaga?cgggcgcgga?cggcggctgg?tggtcggcga?gcgcggggcg 5040
gtggacccgg?ccgggtggat?cacccgcgtc?gggctcgacg?gccgttcgcc?ggacgaggcc 5100
gtcgaggacg?cggcacgcga?ggccgtcggc?aggctcgacc?cggcggcggg?ggtgatggcg 5160
cgggtcgtgt?ggctcgacgc?cggaccggac?cgggccggcc?ggttggtggt?ggtcgtccat 5220
cacctgtcgg?tcgacggtgt?gtcctggcgg?atcctgctgt?cggatctgcg?gtccgcctgc 5280
gaagcggcgg?ccgaggggcg?gaccccggcg?ctggaaccgg?tgggcctgtc?gttcaaacgg 5340
tgggcgagcc?tgctgaagga?gcaggcggtc?tccgcggaac?gggtcgcgga?actggaggcg 5400
tggaaagcga?tcctcggacc?ggtggagcgc?gggccgtccg?agagccgggc?cgcggggggc 5460
gaagtgcgct?cgcggtcgtg?ggtggtgccg?aaggcggaga?cacgggcctt?ggtcggccgg 5520
gctccgctgg?tcttccactg?cggggtgaac?gacgtcctgc?tcgcggggct?ggccggagcg 5580
gtggcccggc?ggcgcggtgg?cgacgcggtg?ctggtggacg?tggaaggcca?cggccgtcat 5640
ccggccgcgg?gcaccgatct?gtcccggacg?gtgggctggt?tcaccagcgc?gcatccggta 5700
cggctggccg?tggcggacac?cgatctggcg?gacgtgctcg?ccggtggccc?ggcggccggt 5760
cggttgctga?agctggtcaa?ggagcagtcg?cgggcggtgc?ccggcgacgg?cctcggatac 5820
ggcatgttgc?gctatctcaa?cgacgtgacg?gggccggtac?tggcggcact?gccgtcgccg 5880
gagatcgggt?tcaactacct?cggcaggttc?gccaccgccg?agaagggcga?cgtgcgggcc 5940
tggcagccgg?tcggcgacct?cggcagtgcg?caggaacccg?gaatgggtct?gccgcacgcg 6000
ctcgaagtca?acgcgatcgt?ccaggattcg?ccggacggtc?ccgaactgac?gctcctgctg 6060
gaatggcagg?aagacctgct?ggacgaagcc?gaggtcgaac?gactggccca?ggactggctc 6120
gacatgctgg?ccgggctggc?ccggcaggcg?gacgatcccg?ccgcgggcgg?gcacaccgcg 6180
tccgacttcg?agctcgtcac?cttggaccag?gccgagatcg?aagccctcga?agccgaatac 6240
gcggacgccg?gcggagtggc?cgacgtgctc?ccgctttcgc?cgttgcagca?cgggctcgcg 6300
ttccacgccg?ggtacggcgg?tgacggggtc?gacgtctaca?ccgcccaggc?ggtgctggac 6360
ctgagcggtc?cgctggacat?gctgctgctg?cggaaatcgg?tgcgcgggct?gctggaccgg 6420
cacccgaacc?tgcgcgccgg?attccggcac?gccgccgccg?ggcccgccta?tcaggtgatc 6480
ccgggcgccg?tggaggtgcc?ggtgtccttt?gtggacgcga?cggggtcggc?ggatccggag 6540
gccgaggcga?cggcggtggc?cgaggccgag?cgggctcggc?cgttcgagct?ggccaagcct 6600
ccgctgctgc?ggatcacggt?ggtggccctg?agccgggaca?gccaccgcct?ggtgctgacc 6660
aaccatcaca?tcctgctcga?cggctggtcg?acgccgctgc?tggtggacga?actgctcacg 6720
ctctaccgcg?acggcgcggc?cccgtccgcg?ctggcgccgg?tcaccccgta?ccgggattac 6780
ctggcctggg?tcggcgaaac?cgatcgggac?gcgtcgatca?ccgcgtggcg?cgaggcgctg 6840
gcgggcctgc?cggaaccgac?cctggtcgcg?gacgaccggc?cggtgccggt?ccaggtgccc 6900
ggccagatgt?gggccgaact?ggacgagacg?ttcgccgccg?ccctgaccgc?gcgggcccgc 6960
gaatgcgggg?tcacggtcag?cacgatgctg?caagcggtgt?ggggcctggt?gctggcggcg 7020
accaccggac?gcgacgacgt?ggtgttcggg?gcggtggtgt?ccgggcgacc?ggccgagctg 7080
cccgggatcg?agaccatggt?cggcctgttc?atcaacaccg?tgccggtccg?ggtccggacg 7140
cggccggagc?agaccttcgc?cgagctggtg?cggcagatcc?aggacgagca?gatcgcgttg 7200
ctggcccatc?accacgtcgg?gctcgccgac?atccagcagg?ccgccgggct?gggccggttg 7260
ttcgacaccc?tcgtcgtcta?cgagaactac?cccaggcccg?acgagaccgc?cgacacggag 7320
ccggatcggt?tgcgggtgcg?gggaatgacc?gccgccgacg?ccacccacta?cccgctggcg 7380
ctggcggtcg?tccccggcga?cgtcctgcgc?ctgcggctgg?agcaccagcc?cacgctgttc 7440
accacggagc?aggccgccgc?cgtgctggac?cgcttcacgc?gggtgctcga?agccgtggtc 7500
gccgagccgc?ggacgccgct?cccggcggtg?ccgatcctgt?ccgccgccga?acagcgggac 7560
ctgctggcgc?gcaacgacac?cgcggtggag?gtgccggacc?ggacgctccc?ggagtcgttc 7620
gccgcgcagg?ccgccgccac?cccggacgcg?accgcggtgg?tcttcgagga?cctgtcgctg 7680
tcctacgccg?aactcgacgc?ccgtgccaat?cagctggcgc?gctggctcat?cgaacggggc 7740
gccgggcccg?aaggcctggt?cgcggtgctg?ctgccccggt?cgctggaact?ggtcgtcacg 7800
gtgctggcga?tcaccaagac?cggtgctgcc?tggctgccga?tcgatccggt?ctatccggcc 7860
gagcgcgtgt?cgtacatgct?cgacgacgcc?cggccgtcgc?tggtgatcac?ctccgcggcg 7920
ctgtcggcgt?cgcccgccgg?tgaggtggtg?gcgggcaggc?aggcgacggt?ggtgatcgac 7980
gatcccgtgg?ccgttggcga?gctggctgcc?cgggatcgcg?ctccggtcac?cgacgcggac 8040
cgcgtccggc?cgctggatcc?gcgtcatccg?gcgtacctca?tctacacctc?gggctccacc 8100
ggcaggccca?aggccgtcgt?cgtcacccac?cggaacctgg?cgaactacct?gttccactgt 8160
ggacggaagt?acccggggct?gcggggacga?tcgctgatgc?attcgtcgat?cgctttcgat 8220
ctgacggtga?ccgcgctgct?cactccgctg?accgtgggcg?gaacggttca?ggtcggtgcg 8280
ctggaagacc?tgatcggcac?ggtggaaagc?gccccggcgc?tgttcctcaa?ggccacgccg 8340
agccatgtga?agacgctgga?caccggttca?cgggagatcg?cggtcgcggg?cgaccttctg 8400
ctcggtggcg?agcagttgcc?cgtcgacacg?atcgcccact?ggcgccggac?ctacccgaac 8460
accgtcgtgg?tcaacgaata?cggcccgacc?gaggccaccg?tcggctgcgt?cgaattccgg 8520
ctcgaacccg?ggcagccggc?gccgtccggc?gaggtggtgc?cgatcggcac?tcccatgacg 8580
aacgcgcggg?cgttcgtgct?ggattcgtgg?ctgcggccgg?tgccgccggg?cgcggtgggc 8640
gaactgtacg?tggcgggcgc?gggtgtggca?cgcggctatc?tcgggcggcc?ggggctgacc 8700
gcgacgcggt?tcgtggccga?cccgtccggg?gacggcgggc?tgatgtaccg?gaccggggac 8760
ctggtgcggt?ggaacccgga?cggggaactg?atgttcatcg?gtcgcgtcga?cgaccaggtg 8820
aaggtgcggg?gttaccggat?cgagcccggc?gagatcgaag?cggcactggt?ggcacaggag 8880
ccggtgggac?aggccgtggt?gctggccaag?gacggcgcct?ccggcaaacg?gctgatcggt 8940
tacgtgaccg?ccgccgggga?gtccgaagtg?gacgaagccg?cggtcctcgc?gggcgtggcc 9000
gcccggttgc?cgcggtacat?ggtgccggcg?gcggtggtgg?tgctggagac?gctgccgttg 9060
acggcgaacg?ggaaagtgga?ccgggacgcg?ttgccggatc?ccgatttcaa?cggacgcgtc 9120
accggacggg?agccggtcac?ggaggccgag?cggctgctgt?gttcgctctt?cgccgaggtg 9180
ctcggactgg?agcgggcggg?cgcggacgac?agtttcttcg?aactgggtgg?cgattcgatc 9240
ctttcgatgc?aactggcggc?gcgtgcccac?cgggcgggaa?tagccttcgg?ggcgcgtgag 9300
gtgttcgaac?agcggacccc?cgcggcgatc?gcggcggtcg?tggaacgggc?ggcgagcgct 9360
cccgccgcga?gtgaacacgc?gacgtccgac?gtcgctcttc?tcgaactgga?ccaagacgag 9420
atcgacgaat?tcgaagcgga?gttcgaaagc?ggtcgctga 9459
<210>4
<211>12153
<212>DNA
＜213〉east amycolatosis
<400>4
atgaaccagt?cgcggattga?ggaaatctgg?ccgctgtcgc?cattgcaggc?cggattgctc 60
ttccacgcgg?tttacgacgg?cgaagggcct?gacgtctacg?tcggtcattg?gatcctggac 120
ctggacgggc?cggtggacgc?ggccaggctg?cgcgcggcct?gggaagcgct?gctgtcccgg 180
cacgccccgc?tgcgcgcttg?tttccggcag?cgcaaatcgg?gcgagacggt?gcagatcatc 240
gccaagcagg?tggaccttcc?gtggcgggag?atcgacctct?cgcaggtcga?agatcccgcc 300
gaagccgtgc?gtgaactggc?cgagcaggac?cggacgacca?gattcgatct?gacgcgcgcg 360
cccttgctgc?ggctcacttt?gatccgcctc?ggcgacgacg?cgcaccgcct?ggtggtgacc 420
tgccatcaca?cgatcatgga?cggctggtcg?ctgcccatcg?tgctggccga?gatgtcgacg 480
ctgtacgcgg?cggacggcgc?tccgacggac?ctgccggaag?tgacgtcgta?ccgggagtac 540
ctggcctggc?tgggccgtca?ggacaaggag?cggtcgctcg?cggcgtggac?ggaacggctg 600
gcgggcgccg?aggagccgac?gctggtggtg?cctgcggatc?cggggcgggc?gccggccgag 660
ccggagagtg?tcgaggccgg?gctgccggag?aacgtcacgc?gagcgctggg?cgaactggcc 720
cgtgccaagg?gattgacgct?gaacacggtg?gtgcagggcg?cgtgggcact?ggtgctggcg 780
cagctgaccg?ggcggacgga?cgtggtgttc?ggggcggcgg?tgtcggcgcg?tccgccggac 840
cttcccggcg?tggaggggat?ggtggggctg?ttcctcaaca?ccgttcccgt?gcgcgtgagc 900
ttgcaggctt?cgacgccggt?cgccggattg?ctgacggagc?tgcagaagcg?gcagtccgcg 960
ctcatccccg?accagtacgt?ggggctggcg?gacatccagc?aggcggccgg?tcccggcgcc 1020
gtcttcgaca?cgttgatcgt?cttcgagaag?ttccaccacg?attccgggga?attgggctcc 1080
gccgagacct?tcggcgtccg?ggtgcatcag?ggccgcgtgg?ccgcccacta?tccgctgacg 1140
ctggtcgcgg?tccccggcga?gtcgatgtac?ttcaagctgg?actatctgac?cgacctcttc 1200
gaccgggaca?ccgcgctcgc?gatcatcgag?cgtttcaccg?gggtgctgcg?cctgctgacg 1260
gacgcgggcg?aggccacggt?cgccgacatc?gaggtgacga?gcccggccga?gcgcgccttg 1320
gtggtcgacg?cctggggcac?gtcgtcccag?gcggcgccga?accggacggc?gccggaactg 1380
ttcgacacac?aggtggagcg?ccaccgcgac?gagccggcga?tcgtcgacgg?cgaccgggcg 1440
ctgtcgtacg?gagaactcgc?cgagcgcgcg?gaacggctcg?ccggtcacct?gcacggcaag 1500
ggagtccggc?gcggcgaccg?ggtggccgtg?gtgctggacc?ggtcacccga?gctgatcgtg 1560
acgttgctcg?cggtgtggaa?ggcgggcgcg?gcgtacgtgc?cggtggatcc?cgcctatccg 1620
gtggaacgcg?tgcagttcat?gctgacggat?tccgggcccg?cggcggtggt?gtgcgcgaaa 1680
acgtaccggg?acgccgtgct?ggacgtcggc?ctcgaaccga?tcgtgctgga?cgatccccgg 1740
acgcggcggg?cggtggagga?gagcgctcgc?cggtcgaccg?ccacgagcgg?cctcgacctc 1800
gcctacgtga?tgtacacgtc?cgggtcgacg?gggacgccga?agggggtcgc?ggtgtcgcac 1860
ggcgacgtcg?cggcgctggc?cggggaaccg?ggctggcggg?tgggccccgg?cgacgcggtg 1920
ctgatgcacg?cttcgcacgc?cttcgacatc?tcgctgttcg?agctgtgggt?gccgctggtg 1980
tcgggcgcac?gggtggtctt?ggccggagcg?ggcgcggtgg?acggcgaggc?gctggcgcgt 2040
cacgtcgccg?acggcgtcac?ggccgcccac?ttgaccgcgg?gggccttccg?cgtgctggcc 2100
gaggaatcgc?cggaatccgt?cgccgggctg?cgcgaggtgc?tgaccggcgg?cgacgcggtg 2160
ccgctttcgg?cggtggagcg?cgtgcggcgg?gtgtgcccgg?acgtgcgggt?gcggcatctc 2220
tacggcccga?ccgaggccac?gctgtgcgcg?acgtggtggc?tgctcgaacc?cggggacgag 2280
ccgggttcgg?tgctgccgat?cggacggccg?ctcgcgggac?ggcgggtcta?cgtgctcgac 2340
gcgttcctgc?ggcccgtgcc?cgcgggggtg?cccggcgagc?tgtacctcgc?cggagccggt 2400
gtggcgcaag?gctatctgga?ccgtccggcg?ttgacggccg?agcggttcgt?cgccgatccc 2460
ttcgcggccg?acgggcggat?gtaccgcacc?ggggacctgg?cgtactggac?ggatcagggc 2520
gcgctggcgt?tcgccgggcg?cgccgacgac?caggtgaaga?tccgcggcta?tcgcgtggaa 2580
ccgggcgaga?tcgaggtggt?cctcggcggt?ctgccggggg?tggcgcaggc?cgtggtgctc 2640
ccgaggggcg?agcatctgat?cggctacgtg?gtcgccgaag?cggggcagga?cgccgacccg 2700
gagcgcctgc?gcgggcagct?cgccgagacc?ttgcccgaat?tcatggtccc?ggccgtggtg 2760
ctggtactgg?acgccttgcc?gttgacggtc?aacgggaagg?tggaccggcg?ggccttgccc 2820
gaaccggact?tcgcgtcgaa?gtcggcgggc?cgggaaccgg?tcaccgacgc?cgaacgagtc 2880
ctttgtggag?tgttcgccga?agtcctcggt?ctcgaccggg?tcgggatcga?cgacagcttc 2940
ttcgaactgg?gcggcgactc?gatctcgtcg?atgcaggtcg?ccgctcgcgc?ccgtcgtgag 3000
ggcatcccgc?tgacgccgcg?gcaggtgttc?gagcaccgga?ctcccgagcg?cctcgcggtc 3060
ttggcgcggg?agaccgccgc?gacgccgcgc?gccgaggccg?tcaccggcgt?gggggagatc 3120
ccgcggacgc?cggtgatgct?cgcgctcgga?gacgacgcgc?tgcgccccgg?tttcgcgcag 3180
gtgcgcgtcg?tcgtcgctcc?ggcgggcctg?gaccaggaca?cgctggcgag?cgcgctgcag 3240
gcggtgctgg?acacgcacga?tctgctgcgg?gctcgggtgg?agccggacgg?acggctgatc 3300
gtgcccgagc?gcggcgcggt?ggacgcggcc?ggtctggtca?cgcgggtggc?cgctggaact 3360
gcggaactcg?acgaggtcgc?cgagcgcgag?ctcaggacgg?cgacgggcac?gctggacccg 3420
tcggcgggga?tcatggcgcg?gctcgtctgg?gtcgacgccg?gggacgccgg?gccgggacgg 3480
ctggccttcg?tggcgcatca?cctggccgtc?gacgcggtgt?cgtggggaat?cctgctgccg 3540
gatctgcgga?cggcttacga?cgagctgatc?tccggccgga?cccccgccct?cgaagcaccg 3600
gcgacgtcgt?accggcagtg?ggcgctgagg?caggtcgagc?aggcgcacag?cgagcgcacc 3660
gtggccgaac?tcgaggactg?ggccgccctg?ctggacggcg?cggacccggc?cggggaagag 3720
gacaccggac?agtcgtattc?gtggtccacg?cgactgaccg?gcaccgaggc?gcgcaacctg 3780
gtgacgctct?tgccgggcgc?gttccactgc?gggatccagg?acgtcctgct?ggcgggactc 3840
gccggtgcgg?tggcgcgggt?acgcggtacc?ggcgccgggc?tgctggtgga?cgtggaagga 3900
catgggcgcg?aagccgccga?cggcgaggac?ctgctgcgca?ccgtcggctg?gttcaccagc 3960
gtccacccgg?tccgcttcga?tctgtccgat?gtggacctcg?cggccgccgg?atcgttgctg 4020
aagaccgcga?aggaccagat?ccgcgccgtg?cccggcgatg?gatccggcta?cgggctgctg 4080
cgtcatctca?accccggcac?cgcggcgacg?ctggccgcgc?tgccgtccgc?gcggatcggc 4140
ttcaactatc?tcggccggac?cgtcctcgcc?ccggaggaca?ccgcgtggga?gccggacggc 4200
gaagggccgc?tcggcggcgg?tccggacatg?gtcctcgcgc?acgccgtgga?ggtcagcgcg 4260
gaggtccagg?acacgccgtc?cggaccccgg?ctggggttgg?ccatcgacgc?ccgggacgcc 4320
ggctccggcc?tggtgcaacg?gctcggtgag?gcgtgggcgg?agacgctgag?cgccctcgcg 4380
gcgctcgccg?gggatcccgg?cgcgggcggg?cacacgccgt?ccgacttcgc?cctcgtcggc 4440
ctgacgcggc?gggacgtgac?ggaactggaa?acggcggcgc?cggggctgac?cgacatctgg 4500
ccgctgtcgc?cgttgcagga?gggcatgctc?ttcgagcggg?ccttcgacgc?cgacggcgtc 4560
gacgtctacc?agagccagcg?gatcctgggc?ctcgacgggc?cgctcgacgc?ggaacggctg 4620
cgcgaggctt?ggacgcgggt?gctcgcccgg?cacgaatcgc?tccggaccgg?ttttcatcag 4680
ctggggtccg?gcgagacggt?gcaggtcgtc?gtgggggagg?ccgaactccc?ttggcgcgtg 4740
gtggatctgt?cgcacctcga?cgaggcggcc?gcggccgcgg?agatcgaacg?gctcctcgcc 4800
gaggatcagg?acaggcggtt?cgacgtgacc?cagccgccgt?tgctgcggct?gtcgctgatc 4860
cggctcggtg?ccgaccggca?ccggctcgtg?ctgacgtccc?accatgtcgt?gctggacggc 4920
tggtcgacgc?cgctcgtcgt?gggtgaggtg?tcggccgcgt?acgcgggcgg?gcagctcccc 4980
gcgaaggcgc?cgtccttcaa?ggactacctg?gcgtggctgg?gccgccagga?cgccgacgcg 5040
acccgggcgg?cgtggcgggc?cgagctcgac?ggcgcggacg?aaccgtccct?tgtggacgcc 5100
gacgcggaca?agacgatggt?gatgcccggg?gagcacgccg?aactgctgtc?cgaggggacg 5160
acgcgcgcgc?tcaccgaact?ggccagggcc?aacggggtga?cgacgagcac?gatcctgcag 5220
gccgcctggg?cgctggtgct?ggcgcggctc?gcgggccgga?cggacgtggt?gttcgggtcg 5280
gtggtggccg?gccgtccggc?ggaggtgccg?gacgtcgaac?gcatggtggg?gatgttcatc 5340
aacaccgtcc?cggtccgggt?gcggctcgac?ggcgggcagc?cggtgctgga?gctgatccgg 5400
gagctgcagg?gacgcctggc?ggcgttgacc?gaacaccagt?acctcggcct?gccggagatc 5460
cagaaggcgg?tgggcacggg?cgcggtcttc?gacaccatcg?tcatgatcga?gaactacccg 5520
cacaccgccg?acggtctcgg?cgacgacggc?ggggtcgcga?tcagctcggt?cgtcacccgc 5580
accggcaccg?gttatccgct?gaccatgaac?gtcagcctcg?ggagccgcct?gcggatcaac 5640
gtgtcctacc?ggcccgaccg?gatcggcgcc?ggcacggccg?ccgaggtcgc?ccggcaggtc 5700
gtgcgggtcc?tggaccaggt?ggtggcacgg?ccttcgctgc?cggtgggccg?cctcggcgtg 5760
acgagcgaac?cggcgcgcga?gtcggtggtg?gaacgctgga?acgcgacggg?cgacgcggcc 5820
gacacgacgt?cggtactgga?gttgttccgg?cgtcaggccg?tcgcctcgcc?ggacgcggtg 5880
gcggtcgtgg?acggggaacg?caccctgtcc?tacaccggcc?tcgaccgtga?atccgaccgg 5940
ctggccggat?atctggccgc?cgtcggggtg?cggcgcggtg?accgcgtcgg?tgtggtgatg 6000
gaacgcggcg?cggacctcct?cgtcgccctg?ctcgcggtct?ggaaggcggg?tgccgcgcag 6060
gtaccggtga?acgtcggcta?ccccgcggag?cggatcgagc?ggatgctggc?cgacgccggg 6120
gtgtcggtcg?cggtctgcct?cgaagcgagc?cgcaaggcgg?tgccggacgg?ggtcgagccg 6180
gtggtcgcgg?atctgccgtc?gctcgacggg?acacggcacg?aggcgccacg?ggtcacggtc 6240
ggggcgcacg?acgtcgccta?cgtcatgtac?acgtcgggat?cgaccggggt?gccgaagggt 6300
gtcgcggtgc?cacacggaag?cgtggcggcg?ctggcgagcg?acccgggctg?gtcgcagggc 6360
cccggcgatc?acgtactgct?gcacgcgtcg?cacgctttcg?acgcgtcgct?ggtcgagctc 6420
tgggtgccgc?tggtcagcgg?ggcccgcgtg?gtggtcgcgg?aaccgggcac?cgtggacgcg 6480
gagcggctcc?gcgaggcgat?cgcccgcggg?gtgaccaccg?tccacctgac?ggcgggtgcc 6540
ttccgtgcgg?tggccgagga?agcgccggac?tccttccgcg?ggctgcggga?gatcctgacc 6600
ggtggggacg?cggtcccgct?cgcctccgtc?gaacggatgc?gccgggcctg?ccccggcgtc 6660
cggttccggc?agctctacgg?tccgaccgag?atcaccctct?gcgccacctg?gctcgttctc 6720
gaaccggggg?cggaaacggg?ggacacactg?ccgatcggcg?gaccgctggc?gggtcggcgg 6780
gcctacgtgc?tcgacgcgtt?cctgcagccg?gtggccccgg?gtgtgaccgg?tgagctctac 6840
ctcgccggag?cggggctggc?gcacggctac?ctcggcaacc?cggtgtcgac?ttcggagcgg 6900
ttcgtcgccg?atccgttcgc?ccccggagag?cggatgtacc?ggaccggcga?cctggcacgc 6960
tggacggaac?agggccagct?gctcttcggc?gggcgtgccg?acgcccaggt?gaagatccgg 7020
ggctaccggg?tcgaacccgg?cgagatcgag?gcggtgctga?ccgagatccc?cgacgtcgcg 7080
caggcgatcg?tggtggcgcg?ggaggaacgc?cccggcgaaa?agcggctgat?cgcctacgtg 7140
accgcggaac?cgggacaggg?actggaatcc?gccgcggtcc?gcgcgcagct?cgcggcccgg 7200
ctgccggagt?tcatggtgcc?gtcggtggtg?ctggtgctgg?acggtttccc?gttgaccatc 7260
aacgggaaga?tcgaccgcgc?ggccctgccc?gcccccgaat?tcgtcccggc?ggcggccggt 7320
cgcgaaccgc?gcaccgggac?cgaacgggtg?ctgtgcgacc?tgttcgccga?aatcctcgga 7380
ctggaccggg?tcggcgccga?cgacggtttc?ttcgaactgg?gcggggactc?gatcctctcg 7440
atgcggctgg?ccgcccgcgc?gcgccgcgag?aacctcgtgt?tcggggcgaa?gcaggtcttc 7500
gaccacaaga?cgcccgcggg?gatcgcggcg?gtcgccgagc?acggcggacg?gacgcgggcc 7560
gaggacggcg?agggcgtcgg?cgaggtcccg?ttgacaccgg?tggtgcgggc?gctgctcgaa 7620
cgcgaccccc?tcggactgac?ccgcggcggc?atggcgcagt?gggtgatcgt?ggacgtgccc 7680
ggcgaccttt?ccgtgaccgc?cctggccgcc?ggactgggcg?cggtgctcga?cacccacgac 7740
ctgctgcgga?gccggctcgt?cgcgaccgag?ggcgaggagc?cccggctggt?cgtggccgac 7800
cggggaaccg?tcgacgcggc?gacgctggtc?gagcgtgccg?ccgcgggcga?catcgacgaa 7860
atcgcgagtg?acgcggcggc?acgcctggat?cccctcgcgg?gcgtgatggt?gcgggcggtc 7920
ctggcggacg?ccgggcggct?cgtggtggcc?gcgcatcacc?tcgtggtcga?cgtggtgtcg 7980
tggcgaaccc?tgctgccgga?cctgcaagcg?gcctgcgagg?ccgcagtcgc?cggcctgcgt 8040
ccgtcgctcg?aagcgccgga?cgtctccttc?cggcgctggt?cgcggacgct?gaccgaagag 8100
gcggcgaacc?ggaccgccga?gctggcgacg?tggacggaga?tcctcgacgg?ggcaccgcgg 8160
cggctcggcg?agctcgatcc?ccggcgtgac?accgtgtcga?ccgcgggccg?ccggacctgg 8220
acccggccgg?cgggcacgct?cgcggacaag?gcgacctcgg?ccttccactg?cggtgtccac 8280
gaggtactgc?tggccaccct?ggccggcgcc?gtgaccggct?ggcggggcgg?caccgccgtc 8340
gtcgtggacg?tcgaaggcca?cgggcgtcag?cccctcggcg?acatggacct?gtcgcggacg 8400
ctcggctggt?tcaccgacgt?ccacccgctc?cggctggaca?cgaccggggc?cgacccggcc 8460
gacgtcgtcg?cgggcggcga?cgcggcgggc?cgtctgctga?agcaggtcaa?ggagaacgtg 8520
cgggcggtgc?ccgacggcgg?gctcggctac?gggatgctgc?gtcatctcaa?cccggagacg 8580
gcaccggtcc?tcgcggcgct?gccgaaggcg?gagatcggat?tcaactacct?cggccgcttc 8640
tccgcacgag?ccgacggtgg?cggcgagcct?tggcggataa?cgggaatcgt?cggtgacgcg 8700
gcggacgatg?ccacgcctct?gcggcacgtc?ctggagatcg?acgccgtcgt?ggcggacggc 8760
gcggacggtc?ccgagttcag?cctgaccgtg?acctgggccc?gccggctcct?cggcgacgcc 8820
gaagccgagt?cgttcgccga?cgcgtggctg?gccgcgctga?ccggcctcgc?cgcgcacgtc 8880
gacggcggcg?gcgggggaca?cactccgtcc?gacttcccgc?tcaccgcgct?gacgcagcga 8940
gaggtggcgc?ggatcgaggc?cgccgtgccg?gatctgctcg?acatctggcc?gctttccccg 9000
ctgcaggagg?gattgctgtt?ccactccacc?ggcgaggacg?gcctcgacgt?ctacgcgagc 9060
atgcggaccc?tcgccctcga?cggcccgctg?gacgccgccc?ggttccgcgc?gtcctggcag 9120
gccatgctcg?accggcacgc?cgccctgcgg?acgagtttcc?accagctccc?gtccgggacg 9180
gccgtgcaga?cgatctcccg?tgaggcgacg?ctgccctggc?aggagaccga?cctgtcccgg 9240
ctgcccgagg?acgaggcgct?ggcggaattc?gaccggctcg?ccgcggcaca?gcacgccgaa 9300
cggttcgacc?tggccaaggg?accgatgctg?cgcctgcacc?tggtgcgcct?cggcgaacgc 9360
aggcaccggc?tgatcttcac?gtcgcatcac?atcgtggtcg?acggctggtc?cctgccgctg 9420
ctcgccaccg?gcgtgctggc?cgtgtacgaa?gcgggcggcg?acgcccgggc?gctgcccccg 9480
tcggcgtcgt?accgcgacta?tctcgcctgg?atcgcggacc?aggacaagac?ggcggcgctg 9540
gaggcgtggc?ggaccgaact?ggcggggctc?gacgaggcga?cccagatcgt?gccgccggag 9600
tcgatcctca?ccccgctcgc?acccgaacgc?gtcaggttcg?ccctcggcga?cgagccgagc 9660
aggcgcctgg?cggagttctg?ccgccgtcac?ggggtcacgc?cgaacacgct?cttccagggg 9720
atctgggccc?tgcacctggc?ccggctgacc?ggacggcggg?acgtggtctt?cggcgccgcg 9780
gtggccgggc?gtccggccga?catccccggg?gtggaggccg?cggtcggcct?gttcatgaac 9840
atgctgccgg?tccgggcgcg?cctcgtcggc?accgaaccgg?tcgtcgacat?gctgaagaac 9900
ctgcaggagc?ggcagttcga?tctgctgccg?catcagcacc?tcgggcaggg?agagatcatc 9960
aaggcggcag?gccggggcgc?ggcgttcgac?acgatcctgg?tgttcgagaa?ctatccgccc 10020
ccgccgcccc?ggctcgacgg?ccccgacgcg?ctcgtcttga?gccccgcggg?gaactcggac 10080
gacgacgggc?actatccgtt?gtccctgcgt?gcttccgtgg?cgggaggtcc?ggtccgcggt 10140
gaagtgatct?accggccgga?cgtgctcgac?cgggccgagg?ccgaggagac?ggtcgcgacc 10200
ctcctccgcg?cactcgagca?ggtgctggcc?gaaccgcgga?cgccggtggg?gcggatcggc 10260
ctgatcggcc?cggagcagcg?ccgcctggtg?gtggacgagt?ggaaccggac?cggcaggccg 10320
gtcaccgaga?agacgctgcc?ggtgctgctg?cgcgaacagg?cggagcggac?gccggacgcg 10380
atcgccgtcc?aggacgcggc?gcgaggcctg?tcgtaccggg?gattggtcgg?cgaagtggag 10440
tcgctggcgc?ggctgctcgc?cgaggcgggc?gtgcgacgcg?aagcccgggt?gggggtgctg 10500
gtcgagcgtt?cggccgacct?cgcggtgacc?ctgctggggg?tcatgttcgc?cggcggggcg 10560
ttcgtgccgg?tcgatccgga?ctatccgcgt?gaccgcgtcg?aattcatgct?ggcgaactcg 10620
gcgccagggg?tgctggtgtg?cacgaaggcg?acccggtcgt?tcgtgcccgc?ggagttcgcg 10680
ggcacggtgc?tggtgctgga?ggacctgccc?gccgccggtc?cggacgccgc?cctgcccgag 10740
ccggcgccgg?aagacgcggc?gtacgtgatc?tacacgtccg?gctcgacggg?cgtgccgaag 10800
ggtgtcttgg?tcacccactc?cggactcgtc?aacctcggct?acgcgcatat?cgaacggatg 10860
gcggtgacgt?cgtcctcacg?gatcctgcag?ttgtccgcga?tcggcttcga?cgccatggtg 10920
tccgagctct?acatgtccct?gctggcgggg?gcgaccctgg?tcctgccgga?cgcggcgagc 10980
atgccgccga?aggtgacgct?gggcgacgcg?atccggcagg?cggagatcac?gcatctgacc 11040
gtgtcgccga?gtgtgctggc?gagcgaggac?gacctgccgg?acacgctgcg?gacggtgctg 11100
acgggcggcg?aggcgctccc?ggccgcgctg?gtggaccgct?ggtcgccggg?ccgccgggtg 11160
atccaggcct?acgggccgac?cgagaccacc?atctgctcga?cgatgagcgc?cccgttgtcc 11220
ccgggacacg?accaggtccc?gctcggcgga?ccgatccaca?acgtggggca?ctacgtgctc 11280
gacgcgttcc?tgcggccggt?gccgcccggt?gtggtcggcg?agctctacat?caccggtgtc 11340
ggactggccc?gcggctacct?cggccgtccc?ggcctgaccg?cggaacggtt?cgtcgccagc 11400
ccgttcgcgc?ccggcgagcg?gatgtaccgc?tcgggcgacc?tgttccgctg?gacccgggaa 11460
ggccagctgc?tcttcgccgg?tcgggtcgac?gcgcaggtca?aggtccgcgg?ctaccgggtc 11520
gagcccgccg?agatcgaggc?cgtgctcgcg?gagcatccgt?gggtcggcca?ggtggcggtc 11580
tccgtccgcc?gggacgggcc?gggcgacaag?cggctggtgg?cctatgtcgt?gccgtcggcg 11640
gccgccggcg?aggggaacgg?cacgctggcc?gccgcgctgc?gggagctggc?cgccgaacgc 11700
ctgccggagt?acatgatgcc?cgcggcgttc?gtgtcgctgg?agaagatgcc?gctcaccccg 11760
aacggcaaac?tcgatcaccg?ggcgctgcaa?gcccccgact?tcgccgggac?gtcggcgaaa 11820
cgggagccgc?gcacggccgc?ggaggcggcg?ctgtgcgcgc?tgttcgcgga?cgtgctgggg 11880
ctcgaccagg?tggctcccga?ggacagcttc?ttcgaactcg?gcggcgactc?gatcacctcg 11940
atgcagttgt?cggcgagggc?ccgccgtcag?gggctggaac?tgaccccgtg?gcaggtgttc 12000
gaggagaaga?cgccggaacg?gctggcgccg?ctcgtccagg?aactcccggc?cgaggacgcg 12060
cccgtcgcgg?taccggagcc?cgccgacggc?acgctactcg?ccctttcccc?tgaccagatg 12120
gacctactcg?aggccgggct?ggccggcgaa?tga 12153
<210>5
<211>5577
<212>DNA
＜213〉east amycolatosis
<400>5
gtgaccgtag?acgacactcg?cgcgaagccc?cgctcgagcg?tggaggacgt?ctggcctctt 60
tcgccgctgc?aagagggaat?gcttttccac?accgccctcg?acaaggacgg?gccggacacc 120
tacacggtgc?agaccgtcta?cgggatcgac?gggccgctgg?acgcgggacg?gctgaaggcg 180
tcgtggcagg?cactcgtgga?ccggcacgcc?gcgttgcggg?cctgtttccg?ctacgtcagc 240
ggcgcgcaga?tggtgcaggt?catcgcgcgg?gacgccgaga?tcccgtggcg?ggagacggat 300
ctttcgggac?tggcggccga?cgccgtcgaa?gccgaagtcg?acaggctggc?cgcggcggag 360
gtggccgagc?ggttgagcgt?cgagacggcg?ccgctgatga?aactgcacct?gatccggctg 420
ggcgaggaga?gccaccggct?cgtgcacacc?ctgcaccacg?tgctgaccga?cggctggtcg 480
atgccgatca?tccaccgcga?gctcgccgag?atctacgccg?cgggaggcga?cgcgtccggg 540
ctgccgccca?ccgtctccta?ccgggactac?ctcgcctggc?tgggacggca?ggacaaggaa 600
gcggcgaagg?cggcctggcg?gaacgagctc?gccgggctcg?acaccccgac?cttggtcgtt 660
cccgccgatc?cggcgcgcgt?gccggatatc?cacaccacgg?tggtcgagct?gtcggcggag 720
ctgaccgacg?gcctcgcccg?gctggcgcgc?ggccacggtc?tcaccctgaa?ctccgtcgtg 780
cagggcgcgt?gggccgtcgt?gctgtcgcag?ctcgccggtc?gcaccgacgt?ggtgttcggc 840
gcgaccgctt?cggggcggcc?cgccgatctc?ccgggcgtgg?agtcgatggt?cggccagttg 900
ctcaacaccc?tgccggtccg?tgtccggctc?gacggcgcga?agcgggcggt?cgacctgttc 960
gacgaggtgc?agcgcggcca?ggcggcgctc?atggcgcacc?agtatctcgg?gctccaggac 1020
gtgcggagcg?tcgccggacc?cggggcggtc?ttcgacacgc?tcgtcatcta?cgagaacttc 1080
ccccgcaagg?gactcgggaa?gccgtcgggt?gacggcctga?acctgctccc?gctgaagcgg 1140
gggagcaact?cctcgcacta?tccgttcacc?ctcgtcaccg?gcccgggcga?acggatgccg 1200
ctcatcctcg?actacgaccg?gggcctgttc?gaccccgcgg?ccgccgaatc?ggtcgtcggc 1260
gcgatggcac?gggtgctgga?gcggctggtc?gccgagcccg?acgtcctcgt?cggccgtctc 1320
accctcgcga?gcgaggccga?acgcgccctg?gtggtggacg?gtttcaacgc?gaccgcgggc 1380
ccggtgccgg?gggactccgt?cgtcgaactg?ttcgcgcggc?gggtggccgg?ggcgccggac 1440
gcggtggcga?tcatcgacgc?cggcggcgcg?aacctgacct?acgccgaagt?cgatcaggcg 1500
tcggatcggc?tggccgggta?tctcgccggt?cgcggtgtcg?gccgcggcga?ccgcgtcggc 1560
gtggccatgg?aaaggtcgcc?ggagctgccc?atcgcgttcc?tggcgatctg?gaaggcgggc 1620
gccgcctatg?tgccggtgga?catcgagtac?ccggccgagc?gcatctcgtt?catcctcgcc 1680
gactccggcg?tctcgaccgt?cttgtgcacc?gaggccacga?aggcggtcgc?tccggaggac 1740
gcgatcgtgc?tcgacgcgcc?cggaacacgc?gcggccgtgg?acgggtgcgc?cgcaccggga 1800
atccggccgc?acgcggacga?tctggcgtac?gtcatgtaca?cctcgggatc?caccggcctg 1860
ccgaagggcg?tggggattcc?gcacggggcc?gtcgccgggc?tggcgggtga?cccgggctgg 1920
cagatcggcg?aggacgacgg?cgtcctgatg?cacgcgacgc?acgtcttcga?cccgtcgctg 1980
tacgcgatgt?gggtgccgct?cgtctccggc?gcccgggtcc?tgctcaccga?accgggcgtg 2040
ctggacgcgg?ccggggtccg?gcaggccgtg?cgacggggcg?cgacctacgt?ccacctcacc 2100
gcgggcacct?tccgcgcact?ggcggaaacc?tcgccggagt?gcttcgaagg?cctcatcgag 2160
atcgggaccg?gcggcgacgt?ggtccccttg?cagtcggtgg?agaacctgcg?gcgggcccag 2220
cccggcctgc?gcgtgcgcaa?cacctacggg?ccgaccgaga?ccaccctgtg?cgcgacctgg 2280
ctgccgatcg?aacccggtgc?cgtgctcggc?tccgagctgc?cgatcggcca?cccgatgacc 2340
aaccgccgga?tctacgtgct?cgacgccttc?ctgcgcccgg?tcgcgccggg?tgtggccggc 2400
gagctgtaca?tcgcgggcac?ggggctggcc?cgcggctacc?tgaacaaccc?cggcctgacc 2460
gccgaccggt?tcgtggcgtg?cccgttcctc?cccggtgagc?gcatgtaccg?caccggcgac 2520
ctggcccgct?ggacccgcga?cggcgagatc?gtgttcctcg?gccgcgccga?cgaccaggtg 2580
aagatccgcg?gttaccgggt?cgagctcggt?gaggtggagg?ccgtgctggc?cgcccagccg 2640
ggagtggtcg?aggcggtcct?gctggcgcgt?gaggaccagc?cgggcgaaaa?gcgccttgtc 2700
ggctacttcg?tgtccgacgg?caccgacgcc?gggccggagg?agatccggcg?gcggatgggc 2760
ctggtcctgc?ccgactacat?ggtcccgatc?gcggtcgtgg?cgctgcccgg?cctgccggtc 2820
acccccaacg?gcaaggtgga?tcggcgggcg?ctgcccgccc?cggacctcgc?gggacgctcg 2880
tcggagaagg?cgccggagag?cgagaccgaa?aaggtgctgt?gcgcgctgtt?cgccgagatc 2940
ctcagcgtcg?agcaggtggg?ggtcgacgac?gccttcaacg?acctcggtgg?cagttcggcg 3000
ctggccatgc?ggctcgtcgc?gcggatccgt?gaggaactcg?gcgaggacct?gcccatccgg 3060
cagctgttct?cctcaccgac?gcccgcgggg?ctggccaggg?ccctggccgc?gaagtcccgt 3120
ccggtactgg?aagccgccaa?gcggccggat?cgggtgccgt?tgaccgcccg?gcagctgcgg 3180
gcctggctgc?tggccgatcc?tggcggggag?acggcgggcc?tgaccacctc?ggtcgcgctt 3240
cgcctgcacg?gcaagctgaa?cgtgcccgcg?ctggtgacgg?cgctcggcga?cgtcgccgag 3300
cggcacgagc?tcctccgcac?gacttttccc?ggtgacgcgc?agagcgtgca?ccagctcgtc 3360
cacgacaccc?cggcggccgg?gccggtcccg?atcccggtga?ccgaggaggc?cctccccggg 3420
ttgctcgccg?agcggcgcga?ggccgccttc?gacctcaccc?gtgaggtgcc?gtggcggtgc 3480
gatctgttcg?agctctcgga?aaaggagcac?gtgctgcacc?tccaggtgca?ccggatcctg 3540
gcggacgacg?attcgctgga?cgtgttcttc?cgcgacctgg?cggccgccta?cggcgcgcgg 3600
cgtgagggcc?gggtcccgca?gcgcgcgccg?ttggccttgc?agttcgccga?ctacgcgctc 3660
tgggagcggc?ggctgctgga?ggacgagaac?gaaccgggca?gcctgatcaa?cgaacagttc 3720
gccttctggc?gggaaaacct?ggccggtatc?gacggcgaga?cggtgctccc?gttcgaccgg 3780
ccgcggcccg?ccgtcccgtc?gcggcgggcg?ggcaccgtcg?cgctgcggct?ggacgccggc 3840
ccgcacgccc?ggctgacgga?ggccgcggag?tcggcgggcg?tggaaacgtc?gcggctggtg 3900
cacgcggcgc?tcgccatgct?gctggccaaa?tacggagcgg?gcgaagacgt?ggtgatcggc 3960
tcggcgctgc?cccgggacga?ggaactcttc?gacctcgaac?cgatggtcgg?accgttcacc 4020
cggccgctcg?ccgtgcgcac?ggatctctcg?ggtgatccga?cccaccggga?ggtcgtcaac 4080
cgggtgcaag?aagccttcca?ggcgaccgaa?cagcatctgg?acgtgccctt?cgagcgcatc 4140
gtcgagctgc?tcgaactgcc?cgcctcgctc?tcccgtcatc?ccgtgttcca?ggtgggtttg 4200
cgggtggacg?aggaggacat?cgacggctgg?acggcggcgg?aactgcccgc?cctgcgcacc 4260
ggtgtcgaac?ccggtggggt?cgaggccatg?gagctggatc?tgtcggtcga?actcaccgag 4320
cttttcgacg?acgacgacga?accgggcggc?ttcgagggcg?tgctgcgcta?cgccaccgac 4380
ctgttcgacg?aggccaccgc?cgagtcggtg?gcccggcggc?tggtccgcgt?cctcgaacag 4440
gtggcggagg?atcccgggcg?gcggatcagc?gagctggacc?tcttccttga?cgacttcgaa 4500
cgctcccgtc?cgcccatcgc?gccggcgcgc?tgggccgggg?ccgtcccgca?ggtggtcgcc 4560
gacgtggccg?gggacgggcc?gctcggcgcg?ctcgtactgg?acgagcggtc?gcgccccgtc 4620
gccccgggca?ccgtcggcga?gctctacgtc?accggtcccg?cggtggacga?gggaccggcc 4680
gatctcccga?ccgtgccctg?tccgttcggg?gaggaggggc?accggatgct?gcggaccggt 4740
ctgctcgcca?ggaagacgcc?cgccaagacc?ctggtcgtcg?tcggcgagcg?gcggcggacg 4800
agctcttcgg?tgaagacggg?cgacttcgag?atcctgctgc?cgctccggac?gaccggggac 4860
cggcccccgc?tcttctgtgt?ccacgcgagc?ggcgggctga?gctggaacta?cgagccgctg 4920
ttgcgtcacc?tcccgtcgaa?ccagccggtc?tacggcgtcc?aggcccgcgg?tctcgccagg 4980
accgagccgc?tgccgggcag?cgtcgaggag?atggccgccg?actacctcgc?gcagatccgt 5040
gccgtgcagc?cggaagggcc?gtatcacctg?ctcggctggt?cgctcggcgg?ccgtatcgcg 5100
caggcgatgg?ccaaactgct?ggaggaggaa?ggggagaagg?tcggcctgct?cgccttgctc 5160
gacgcctatc?ccgtctacat?gggacggaag?acgaccgggg?cggcgagcga?ggaagaggcg 5220
ctgtcgaagc?ggaaccaggc?ggacctcgac?ctcgcgggcc?agctggtcaa?gggcgaggcc 5280
gcccgtgccc?gcctggaggc?ggtcatgcgc?aatctctggg?acgtcgggcc?gcgccacgtc 5340
tcttccccct?tcgccggcga?catcctgctc?ttcgtggcca?ctgtggaccg?tcccgagcat 5400
ttgccggttt?cggtggcgaa?ggccagctgg?aaggacttca?ccagcgggac?catcgagacc 5460
cacgacgtcc?cgtccaacca?ctacgacatg?gtgcaaaccg?cggcgctggg?ccagattgga 5520
gccatcgtcg?ccgagaaact?ccggcagcgt?ccggaaggtg?aaaggacaca?acgatga 5577
<210>6
<211>1176
<212>DNA
＜213〉east amycolatosis
<400>6
gtgttcgagg?agaagaacgc?cctccggggc?acggagatcc?accggagaga?gcgcttcgat 60
ccggggccgg?aactgcgctc?cctgatggcc?gagggccgca?tgtccgtgat?ggagtccgaa 120
gacggcccag?acgggcggac?cgggtggctc?gccaccgggt?acgaagaaac?ccgccaggtg 180
ctcggctcgg?acaagttcag?cgccaagctg?ctcttcggcg?gcaccgcggc?gggccggatc 240
tggcccggct?tcctcaacca?gtacgacccg?ccggaacaca?cgcggctgcg?ccggatggtg 300
gcgtcggcgt?tcaccttccg?gcgcatgcag?gacttccggc?cgaagatcga?ggcgatcgtc 360
gaggccacgc?tggacgacat?cgaagccacc?ggcgggccgg?tggacttcgt?gccgcggttc 420
gcgtggcccg?tggcgacgac?ggtgatctgc?gacttcctcg?gcatcccgcg?tgacgaccag 480
gccgacctgt?cccgcgccct?gcacgccagc?cgggccgaac?ggtcgggcaa?acggcgtgtg 540
gcggcgggga?acaagtactg?gacgtacatg?ggccagatcg?cggccaagac?gcgccgcgat 600
cccggtgacg?acatgttcgg?cgccgtggtg?cgcgagcacg?gcgacgacat?caccgacgcg 660
gaactgctgg?gcgtggccgc?gttcgtcatg?ggggcgagcg?gggaccaggt?ggccaggttc 720
ctcgcggcgg?gggcgtggct?gatggtcgag?caccccgagc?agttcgccgt?gctccgggac 780
aagccggaaa?ccgtcccgga?ctggctgaac?gaggtggccc?ggtacctcac?cagcgacgag 840
aagaccactc?cgcgcatcgc?gcgggaggac?gtgtacatcg?gtgaccagct?ggtgaagaag 900
ggcgacgccg?tcacctgctc?gctgctggct?tcgaaccgcc?ggcagttccc?cgacccggag 960
gaccggttcg?acatcacccg?ggaaaagccg?gcgcacgtca?ccttcgggca?cggcatccat?1020
cactgcctcg?gcaggccgct?ggccgaaatg?gtgttccggg?cggcgatccc?gggcctggcg?1080
caccgctttc?ccaagctccg?gctggccgaa?ccggaccgcg?agatcaaact?gggcccgccg?1140
ccgttcgacg?tcgaatccct?gctgctcgac?tggtag 1176
<210>7
<211>1224
<212>DNA
＜213〉east amycolatosis
<400>7
atgacacgtt?cgcaaaggag?tttcccgttg?agtgaggacg?acccgcgccc?gctgcacatt 60
cgcagacagg?ggctggaccc?ggcacccgaa?ctgctcgccg?ccgggccgct?gacgagggtc 120
accatcggct?ccggagcgga?cgccgaaacg?cattggatgg?ccaccgcgca?cgccgtcgtc 180
cggcaggtga?tgggcgatca?caagagtttc?agcacccggc?gtcgctggga?cccccgggac 240
gagatcggcg?ggaagggcat?cttccggccg?cgggaactgg?tcggcaacct?gatggactac 300
gacccgcccg?agcacgccag?gctgcggcag?aagctgaacc?cgggtttcac?gctgcgcaag 360
atgcagcgga?tggggccgta?catcgaacag?atcgtgaccg?aacggctgga?cgaactggag 420
caggcgggat?cccccgcgga?tctggtcggg?atcgtcgccg?acaaggtccc?cggcgccgtg 480
ctgtgcgaac?tgatcggtgt?gccgagggac?gaccgcgcca?cgttcttgca?gctgtgtcac 540
gcgcatctcg?acgcctcgcg?cagccagaag?agacgcgcgg?tggcgggtga?ggcgttctcc 600
cgctacctgc?tggcgatgat?cgccagggaa?cgcaaggaac?cgggcgaagg?cctgatcgga 660
gcggtcgtcg?ccgagtacgg?cgacgaagcc?acggacgagg?aactgcgcgg?cttctgcgtt 720
caggtgatgc?tggccggcga?cgacaacatc?tccggcatga?tcggcctcgg?tgcgctggcc 780
ctgctgcgga?accccgggca?gatcgccgcg?ttccaaggcg?acgagcagca?ggcgcaacgg 840
gcggtcgacg?agctgatccg?gtacctgacg?gtcccgtatt?ccccgactcc?ccggatcgcc 900
atgcgggaca?tcaccatcgc?gggccaggcg?atcaaaaagg?gcgagagcgt?catctgttcc 960
ctcccggcgg?ccaaccggga?tcccgcgctg?gtgccggatc?ccggacgcct?cgacgtcacc 1020
cgcgagtccg?cgccgcatgt?cgcgttcggg?cacggggtcc?atcactgcct?gggcgccgca 1080
ctgacccggc?tccagctgcg?caccgtgttc?accttgctgt?ggcggcgttt?tcccgagctg 1140
cggctggcgg?atcccgcgca?ggaaaccaag?ttccgcctga?ccaccccggc?gtacggattg 1200
accgagctga?tggtcgcctg?gtga 1224
<210>8
<211>1221
<212>DNA
＜213〉east amycolatosis
<400>8
atggggcacg?gtatcgaaca?ggtcgcgcca?ctcctggagg?agcccgcgaa?ctttcagctg 60
agaacgaact?gcgatccgca?tacggacaat?ttcgcactga?gggcgcacgg?cccgctcgtc 120
cggatagccg?gcgattcctc?ggcccagctc?ggccgggatt?tcgtctggca?ggcccacggc 180
tacgacgtcg?tgcgcaggat?cctcggtgac?cacgagaatt?tcacgacgcg?cccgcaattc 240
acccaggcga?aatccggggc?gcatgtcgag?gcccagttcg?tcgggcagat?ctcgacctac 300
gatccgcccg?agcacacccg?gttgcggaag?atgctgacac?cggagttcac?cgtccggcgg 360
atccgccgga?tggagcccgc?gatccagagc?ctcgtcgacg?atcgcctcga?catgctggag 420
gccgaggggt?cgcccgcgga?cctccagggc?ctgttcgccg?atccggtcgg?cgcccacgcc 480
ctgtgcgaac?tgctcggcat?cccccgcgac?gaccagcgcg?agttcgtccg?gcggatcagg 540
cggaacgccg?atctgagccg?cggcctcaag?gcgcgggccg?ccgacagcgc?ggcgttcaac 600
cggtacctgg?acaacctcat?cgcccggcag?cgcgcggatc?ccgacgacgg?actcctcggc 660
acgatcgtgc?gagagcacgg?cggcaacgtc?acggacgagg?agttgcgggg?cctgtgcacg 720
gcgctgatcc?tcggcggtgt?cgaaaccgtc?gcggggatga?tcggcttcgg?cgtgctcgcc 780
ctccttcagc?accccggcca?gataccgttg?cttttcgaag?gcccggaaaa?ggccgaccgc 840
gtcgtcaacg?agctggtgcg?ctatctgtcc?cccgtgcagg?cgccgaatcc?ccggctcgcc 900
atcaaggacg?tggtcgtcga?cgggcagctg?atcaaagcgg?gggactatgt?cctgtgctcg 960
gttctcatgg?cgaacaggga?cgaagcactg?acccccgatc?cgaacgtttt?cgacgcgaac?1020
cgcaccgctg?tttcggacgt?cgggttcggg?cacggaatcc?attactgcgt?cggtgccgct?1080
ttggcccggt?cgatgctgcg?gacggcctat?cagaccttgt?ggcggcgatt?cccggggctg?1140
cggctggccg?tgcccgtcga?agaagtgaaa?taccgcagcg?cgttcgttga?ctgccccgat 1200
caagtaccgg?tcacctggtg?a 1221
<210>9
<211>1476
<212>DNA
＜213〉east amycolatosis
<400>9
atgtcggtcg?aagatttcga?tgttgtggtg?gcgggcggcg?ggccgggtgg?ttcgacggtg 60
gccaccctgg?tggcgatgca?gggacaccgg?gtcctgctgc?tggagaaaga?ggtcttcccc 120
cggtaccaga?tcggtgagtc?gctgctgccc?gccacggtgc?acggggtctg?ccggatgctc 180
ggcgtcgcgg?acgagctggc?gaattccggg?ttcccgatca?aacgcggcgg?cacgttccgc 240
tggggcgccc?gtccggagcc?gtggacgttc?cacttcggga?tctcggccaa?gatggcgggc 300
tcgacgtcgc?acgcctatca?ggtcgagcgg?gcgaagttcg?acgacatcct?gctgaagaac 360
gccaagagca?agggcgtcgt?cgtgcgggaa?ggctgctcgg?tcaacgacgt?cgtggaggac 420
ggcgagcggg?tcaccggcgc?gcgctacacc?gacgcggacg?gcaacgcgca?cgaagtctcg 480
gcccggttcg?tgatcgacgc?gtcgggcaac?aagagccggc?tctacacgaa?ggtcaacggt 540
tcgcggaact?actcggagtt?cttccgcagc?ctcgcgctgt?tcggctattt?cgagggtggc 600
aaacggctgc?ccgagccggt?gtcgggcaac?atcctgagcg?tcgccttcga?cagcggctgg 660
ttctggtaca?tccccctgag?cgacacgctg?accagcgtcg?gcgcggtcgt?gcgccgggag 720
gacgccgaca?agatccaggg?cgaccgcgag?aaggccctca?acaccttgat?cgccgaatgc 780
ccgctgatct?cggagtacct?ctcgaacgcg?accagggtga?ccaccggcag?gtacggcgaa 840
ctgcgggtgc?gcaaggacta?ctcgtaccag?caggacagct?actggcggcc?cgggatggtc 900
ctggtcggcg?acgccgcgtg?cttcgtggac?ccggtgttct?cctccggggt?gcacctggcg 960
acctacagcg?cgctgctcgc?ggcccggtcg?atcaacagcg?tcctcgcggg?cgacctcgac 1020
gagaagaccg?cgctgaacga?gttcgaggcg?cgctatcgcc?gcgagtacgg?cgtcttctac 1080
gagttcctcg?tctccttcta?tcagatgaac?gtcaacgagg?aatcgtattt?ctggcaggcc 1140
aagaaggtca?cgcagaacca?gagcaccgac?atcgagtcgt?tcgtcgagct?gatcggcggg 1200
gtgtcgtccg?gcgagaccgc?gctgacggcc?gccgaccgga?tcgccgcgaa?cagtgccgaa 1260
ttcgccgccg?ccgtcgacaa?gatggcgacg?ggcgacggcg?acgacatggt?gccgatgttc 1320
aagtcgaccg?tggtcaagca?ggcgatgcag?gaggcgggcc?aggtccagat?gaaggcgctg 1380
ctcggcgagg?acgccgaacc?cgagctgccg?ctgttccccg?gcggcctggt?gacttcgccc 1440
gacgggatga?agtggctgcc?gcaccacccg?gcctga 1476
<210>10
<211>1227
<212>DNA
＜213〉east amycolatosis
<400>10
atgcgtgtgt?tgttgtcggt?gtgcggaacc?cgcggggacg?tcgagatcgc?ggtgtcgctg 60
gcggtccggc?tgaaggcgct?cggcgtcggg?acgcggatgt?gcgcaccgcc?cgccgccgcc 120
gagcggctgg?ccgaggtcga?ggtgccgcat?gtgccggtcg?gccttccgca?gcacatgatg 180
ttgcaggagg?ggatgccgcc?gccgcccccg?gaggaggagc?ggcggctcgc?ggccatgacg 240
gtcgagatgc?agttcgacgc?gatcccctcg?gccgccgaag?gatgcgtggc?ggtggtggcg 300
gtcggcgatc?tggccgccgc?gaccggcgtg?cggtcggtgg?ccgaaaagct?gggcctcccc 360
ttcttctact?ccgtcccgag?cccggtctac?ctggcttccc?cgcacttccc?gccgccctat 420
gacgagccga?ccaccccggg?cgtgaccgac?caccggacac?tgtgggaaga?gcgtgcccac 480
cggttcgcgg?aacggtacgg?ggagacgctc?aaccggcggc?gggccgcgat?cggcctgccg 540
ccggtggagg?acgtcttcgg?ctacggccac?ggcgaccggc?ccatcctgtc?ggcggacccg 600
gtcctcgccc?cgctgcagcc?ggacgtcgac?gccgtgcaga?ccggcgcgtg?gatcctgacc 660
gacgaccggc?cgcttccccc?ggagctggag?gcgttcctgg?ccgccggccc?accgccggtg 720
cacgtgggtt?tcggcagctc?gtccgggaag?gggatcgccg?acgccgcgaa?gatcgccgtc 780
gaggtgagcc?gtgcccacgg?ccgccgggtg?atcctctccc?gagggtggac?cgatctgctc 840
ctgcccgacg?accgggagga?ctgtttcgcc?atcggcgagg?tgaacttcca?ggcgctgttc 900
ccccgggtgg?ccgccgtcat?ccatcacggc?agcgcgggca?cggaacacgt?ggccacgcgg 960
gcgggcgtcc?cccagctcgt?gatcccccgg?aacaccgacc?agccgtactt?cgccgccagg 1020
gttgccgatc?tggggatcgg?tgtggcgcac?gacggcccga?cgccgacctt?cgagtccctg 1080
tcggccgcgc?tcaccacggt?cctggcgccg?gaaacgcgcg?cgcgggcgcg?ggccgtggcg 1140
gccatggccc?agaccgacgg?cgcggcggcg?gccgcggatc?tggtgctcgc?cgccgtcggc 1200
gggaacgagc?ccgccgttcc?cgcgtga 1227
<210>11
<211>1227
<212>DNA
＜213〉east amycolatosis
<400>11
atgcgtgtgt?tgttgtcgac?ctgtgggagc?cgcggagacg?tcgaaccact?ggtggcgttg 60
gcggtgcggt?tgcgggagcg?cggcgccgag?gtgcggatgt?gcgcgccgcc?ggactgcgcg 120
gatcggctgg?ccgaagtcga?cgtgccgcat?ctgcccctcg?gtgcgtcggc?gcgcccgtcg 180
gccgggcagg?cgaaaccctt?gacggccgag?gacatgctcc?ggttcacgac?cgagacgatc 240
gccacgcagt?tcgagcggat?tccggcggcc?gccgaaggat?gcgccgcggt?ggtgacgacc 300
ggcctgctgg?ccgccgccat?cggcgtgcgg?tcggtggccg?aaaagctggg?catcccctac 360
ttctatggct?tccactgccc?gagctatgtg?ccgtcgccgt?actatgcgcc?tccgccgccc 420
ctcggcgagc?cgcccgcacc?ggacgggacc?gacatccagg?cgctgtggga?gcgcaacaac 480
cagagcgcct?accggcggta?cggggagccg?ctcaacagca?ggcgcgccgc?catcggcctg 540
ccgccggtgg?aggacatctt?cggccacggc?tacaccgatc?acccgtggat?ggcggcggac 600
ccggtactgg?ccccgctgca?acccacggat?ctcgacgccg?tgcagaccgg?ggcgtggatc 660
ctgcccgacg?aacgaccgat?ttccgctgag?ctggaggcgt?tcctggacgc?cggcgcacca 720
ccggtgtacc?tggggttcgg?cagccttcgc?gcccccgccg?acgccgcgaa?ggtggccatc 780
gaggcgatcc?gtgcccacgg?ccaccgggtg?atcctctccc?gcggctgggc?cgatctggtc 840
ctgcccgacg?accgggagga?ctgtttcgcc?atcggcgaag?tgaatcagca?ggtgctgttc 900
cgccgggtgg?ccgccgtcat?ccaccacggc?ggcgcgggca?cgacccacgt?ggccacgcgg 960
gcgggcgtcc?cccagatcct?ggttccccag?atcgcggacc?agccctacta?cgccgcccgg 1020
gtggccgaac?tgggggtcgg?tgtggcgcat?gacggcccga?ccccgacctt?cgacacgttg 1080
tcggcggcgc?tcaccaaggc?cctcgctccg?gaaacgcgcg?tgcgagcgga?agccgtggcg 1140
gaaacggtcc?agacggacgg?ggccgcggtg?gccgcggacc?tgttgttcgc?cgcggtgacc 1200
gggaaccagc?ccgccgttcc?cgcctga 1227
<210>12
<211>1227
<212>DNA
＜213〉east amycolatosis
<400>12
atgtcgacca?cttcccggtg?ccgcatctgc?gacggcaccg?tcaccgagtt?catcgatctc 60
ggacgccagc?cgctttcgga?cgcgttcgtg?gttcccggcg?acgagaagaa?ggagttcttc 120
ttccggctcg?ccacggggat?ctgcgagtcc?tgcacgatgg?tgcagctgat?ggaagaggtc 180
ccgagggacc?tgatgttcca?cgaggcgtac?ccctatcact?cgtccggttc?gtcggtcatg 240
cgcacgcatt?tccaggaact?ggccaagcgc?ctgctgacca?cggagctgac?cggcgaggac 300
ccgttcatcg?tcgagctggg?gtgcaacgac?ggcatcatgc?tcaaggccgt?ggccgaagcc 360
ggggtacgcc?agctcggtgt?ggaaccctcc?ggcggcgtcg?cggatctggc?cgcggccaag 420
ggaatccgcg?tccgcaagga?tttcttcgaa?gaggcgacgg?cggccgacat?ccgggagaac 480
gacggccccg?ccgacgtgat?ctacgcggcc?aacaccctgt?gccacatccc?ttacatggac 540
tcgatcctca?agggcgtcac?gacgctgctg?cgcccgaacg?gcgtgttcgt?gttcgaggac 600
ccgtacctcg?gcgacatcgt?ggagcgcacg?tcgttcgacc?agatctacga?cgagcatttc 660
ttcttcttca?ccgcgcgctc?ggtccaggag?atggcccggc?gcaacggtct?cgaactcgtg 720
gacgtcgagc?gcatcccggt?gcacggcggc?gaggtccgct?acaccctcgc?cctcgccggg 780
gcccgtaagc?cttcctcggc?ggtggcggaa?ctcctggcgt?gggaggccga?gcgcaagctg 840
gcggagtacg?ccacgctgga?acgcttcgcc?accaacgtga?agaagatcaa?ggaagacctg 900
atcgcgctgc?tgaccaagct?ccgcgccgag?ggcaagcgcg?tcgtcggcta?cggcgcgacg 960
gccaagagcg?ccacggtgac?caacttctgt?ggcattaccc?cggatctggt?ggagttcatc?1020
tcggacacca?ccccggccaa?gcagggcagg?ctcagccccg?gacagcacat?cccggttcgc?1080
gagtaccagg?cgttcgccgg?cgaccacccg?gactacgcgc?tgctgttcgc?ctggaaccac?1140
gccgacgaga?tcatgaacgc?ggagcaggcc?ttccgcgacg?ccggcgggca?gtggatccgg?1200
tacgtgccga?acgtgcacgt?gagctga 1227
<210>13
<211>843
<212>DNA
＜213〉east amycolatosis
<400>13
atgaccgatc?aactggaccg?cggcccggcg?cggaccgcgc?acgccgatgt?cgtcctggcc 60
tcggcgggtg?accggggcgt?tctgtgcgat?ttctacgacg?aggcgggctc?ggacacctat 120
cgggatttga?tccaggacgc?cgacggcacc?ccggaaacgc?gggaattcgc?ggcccgtctt 180
cccgaggatc?cggggcccgt?gctggagctg?gcggccggaa?tgggcaggct?gacgttccct 240
ttcctggagc?tcggctggga?gatgaccgca?ctggagctgt?cggcgacggt?ggtcaccgct 300
ttccggaagc?ggctggcgga?atcgccgtcg?gagctccgag?agcggtgcac?agtggttcag 360
ggggacatga?gcgccttcgc?gttgggcgag?cgtttcggcg?cggtggtcat?cagctcgggt 420
tcgatcaacg?aactcggcga?ggccgaccgg?aagggcctgt?acgcgtcggt?tctcgagcat 480
ctgcggcccg?gcgggaagtt?cctgctcagc?ctggaattga?cgaagcccgt?cgaagcggag 540
ccggtggagc?gcagccagga?gctgaccggc?cggagcggca?ggaggcacgt?attgcacgtg 600
aagatcctgc?cgtcggagga?gctccaggag?atcaccatcc?accccgccga?cgagaccgcg 660
gatcccttcg?tcgtctgcac?gcatcgccgg?aggctcttca?cggtggaccg?gatcgcgcgg 720
gaactcgggc?aggccggctt?cgacgtcgtc?gcgcgggaac?cgttcgcctc?caccggcacc 780
ggtcgtgacg?acatggtgct?ggtggaggcg?atcccgcgag?aggtcacggc?agacgcgaag 840
tga 843
<210>14
<211>1305
<212>DNA
＜213〉east amycolatosis
<400>14
tcacggcaga?cgcgaagtga?tgagcgcggc?gagcctgctc?acgccctcct?cgatcagctc 60
cggcgtgagc?aggctgatgg?acagccggag?ctggttgaac?ccgtccttcc?cgccgtagaa 120
gtgatgcatc?ggggtgaaca?gcacaccgtg?gtcgcgggcg?gcgtccgcca?gcagctcgtc 180
gtcgacgatg?aagggcacgg?tgacggtgac?gaagaacccg?cccgtcgggg?tgttccagcg 240
gaccccgggg?cggtcgccga?gcctgcggtc?gagctcgctc?agcgtcagct?ggagattccg 300
ctggtagatc?gcggtttccc?ggacgttggc?cttggtcagg?ctgtagtcgt?tgagcagcag 360
cttcccggcg?atcaccgcct?gcgcgatggg?cgaggtgttc?acggtgagca?tgcccttgag 420
cttcgagagc?tggtcggcgt?acaggccacc?gcccgccacc?cgctgatccg?ccaccgcgta 480
gccgacccgg?gcgccgggca?tgccggtctt?ggcgaaggat?ccgatgtaga?ccacgttccc 540
cgaccggtcg?agcgctttca?gcgtggggag?gcgctcggcg?ccgaagagcc?cgtacgcgtt 600
gtcttccagc?agaaggatcc?cgtgggccgc?ggcgacttcg?agcagccggt?ggcgggacgg 660
cagatccatg?ctggtgccgg?tggggttggc?gaagttgggc?gtcacgtagc?aggcccgcac 720
ccgcatgccc?tgttcgtcgg?cgcgcttcag?ctggaggacg?agatcctcgg?ggtcgatgcc 780
gttctcgccg?gaccggaccg?gccagaccgg?cgtgtcggtg?agcagcgccg?ccccggtcag 840
gccgacgtag?gtgggcgccg?gggcgagcag?cacgtcccgc?tcgttcgccc?gcagggtgcg 900
gagcaccagg?aacatggcct?cctgggcgcc?gacggtgacg?accaccgatt?ccggggcggc 960
gtcgatgttc?tcgtcctcgg?ccaggttgcg?ggcgatgatg?tcggcgatga?tgcccttcgt 1020
ggtgccgtat?tggaaaagcg?cgcgggtgac?gccggcctcg?tccactttcc?gatcgcgccg 1080
gagatgctcg?cagtacgcgt?cgagataatc?gtgaatgagc?cggatgtcga?aaaactcttc 1140
gtatggccgt?cctgccgcca?tggaaatggc?cacagggtat?tcgtcgatca?gttcattgag 1200
cagattcatc?gacgagatgg?ccggatcggt?gagcgatccg?tgcagcgttt?ccatgtccaa 1260
atgggtggac?aaaacgttcg?aatccataaa?tgctaggatt?tccac 1305
<210>15
<211>831
<212>DNA
＜213〉east amycolatosis
<400>15
atgctgatga?caaccgagaa?cgggatccgg?ttgtcttacc?acgatcaggg?caacggcccg 60
gccgtcctgt?tgctgaccgg?caccggcgcg?ccgagttccg?tctgggatct?gcaccaggtt 120
cccgtgctcc?gcgccgccgg?attccgcgtg?atcaccatgg?acaaccgcgg?gatcccgccg 180
agcgacgagg?gcacggacgg?gttcaccatc?gacgacctcg?tcgcggacgt?ggcggcgctg 240
atcgactacc?tcgacgccgt?gccgtgccgc?gtgatcggca?cgtcgatggg?ttcgtacgtc 300
gcgcaggagc?tggcactcgc?ccgtccggaa?ctgctggact?cggtcctgtt?gatggcggcc 360
tgcggcaaga?gcagcctcgt?ccagcgagaa?ctcgcggaag?gcgaagcgaa?gctgatcgaa 420
caaggcatcg?agctgccacc?ggccttcctc?gccgccgtcc?gggccatgca?caacctggga 480
cccgcgaccc?tcgccgacga?cgacctcgcc?ggcgactggc?tcgacctgtt?cgccgcggcg 540
ggatcctggg?ggccgggtgt?gcgggagcag?ctgctgctga?gcgcgttgcc?cgaccgcctc 600
gaggcctacc?gctcgatcaa?ggtgccctgt?cacgtcgtct?cgttcgaaca?cgacctcgtg 660
gcgccgccgt?ccgccggacg?cgagctggcg?gacgcgatcc?ccggcgcgac?ccaccgcacg 720
attcccgggt?gcgggcattt?cggctacctg?gagaacccgg?aggcggtgaa?ccgggagctg 780
atccggttcc?tccgcaccga?atcccgtgag?actttggggg?agaccgcatg?a 831
<210>16
<211>1683
<212>DNA
＜213〉east amycolatosis
<400>16
gtgctgccgg?atcggccggc?ggtggcgatg?gacaccacca?cactcaccta?cgccgaactc 60
aacggcgaag?ccaaccggct?cgcgcgacgg?ctcgtggcgc?acggcgtggg?cccggagcgg 120
ctggtcgcgc?tggccatgcc?gaggtcgatc?gagttcgtca?tcgcgatcct?ggccgtgcac 180
aaggcgggcg?ccgcgtacgt?gccggtcgac?ccggactatc?cggaggaacg?caaacgccac 240
atgctggacg?acacggcggc?gcagctcgtg?ctgtgcctgc?cggggcagga?agtgcccggt 300
gacggcgtcg?tcctgagcgt?ggaacgggaa?ccggggctgt?ccgagccgaa?cctggacgac 360
ggggaccgcc?tcggcccgtt?gctgcccgac?catcccgcgt?acgtcatcta?cacctcgggc 420
tcgaccggga?aaccgaaggg?cgtgctggtc?acgcatcgcg?ggatcccgaa?cctggccgac 480
gactacgtgc?gccgccagga?actggtgccg?gacagcaggt?tgctggcctt?cgcctcgccg 540
agcttcgacg?ccgcggtcgc?cgagttctgg?ccgatctggc?aggccggtgg?ctgtctcgtg 600
ctggcctccg?cgccggacct?ggtccctggc?gagccgctcg?ggaggctggt?gcgagagcag 660
gggatcaccc?acatcacgct?gccgccgtcg?gcgctggcgc?cactggagga?ggcgggcggc 720
ctgcccgccg?ggctgaccct?cctggtggcg?ggcgaggcgt?gcccggcgcc?ggtggccaaa 780
cgctggtcca?tggaccgcgt?gatgatcaac?gcctacggcc?ccaccgaagt?caccgtggcg 840
gtgaccgcga?gcgagccgct?gtccggtgag?gagaccccgc?cgatcggcag?gccgatcacc 900
ggtgtgcgca?cctatgtcct?ggacgaactg?ctccggcccg?tcccggacgg?ggacgtgggc 960
gagctgtaca?cgatgggtcc?cggcctcgcc?cgcggatatc?tcaggcggcc?cgccgcgacc 1020
gcggagcggt?tcctgccgga?tccgttcggc?gaaccgggcg?ggcgcatgta?ccgcaccggc 1080
gaccgggtgc?ggcggcgtcc?ggacggccag?ttcgtcttcg?tcggccgcgt?cgacgaccag 1140
gtgaaggtcc?gcggtcaccg?gatcgaaccg?ggcgaggtgg?agtccgcctt?gctcgccgtg 1200
gacaaggtgg?cccaggcggt?ggtgaccgcg?cacgacaacc?ggctcgtcgc?ctacgtcgtc 1260
ggcaccgggg?gcgaacgcgt?gacggccgaa?gagctcctcc?cgcccctgcg?ggagcggatg 1320
ccgtcctacc?tggtccccga?cgtggtcgtc?agcctgccga?gactgccggt?ctcgccgaac 1380
ggcaaggtcg?accggaccgc?cctgcccacc?ccggaagagg?agagcgccga?gcgcgcggcg 1440
tcgggacggg?cgccgagcac?accgacggaa?atccttctcg?ccgaactgtt?cgccgacgtg 1500
ctcggtgtcg?ccagtgtcgg?ggtcgaggac?agcttcttcg?agatcggtgg?tcattcgctg 1560
ctcgcgaccc?gggtggtgag?ccggatccgc?gaaagcctga?agatccggct?gagagtgcag 1620
gccttcttcg?acgcgccgac?cgtggcacag?ctcgccaagg?tgctcgacgg?cgcactcacc 1680
tga 1683
<210>17
<211>1191
<212>DNA
＜213〉east amycolatosis
<400>17
atgcagacga?cgaaaaccgt?cgacctcggc?gatcccgatc?tgtacaccac?cctcgaccgt 60
cacacccgct?ggcgcgagta?cgcggcggaa?gacgcgatgg?tgtggagcga?accgggcagt 120
tcacccggcg?gcttctggtc?ggtgttctcg?caccgggcgt?gcgccgcggt?gctcggcccg 180
tccgccccgt?tcacctccga?gtacgggatg?atgatcggct?tcgaccgcga?gcacccggac 240
aattccgggg?gccagatgat?ggtggtgtcc?gaacaggacc?agcaccgcaa?actgcggaag 300
ctggtcggac?cgctgctttc?ccgggcggcg?gcgcggaaac?tgtcggaccg?ggtgcggacc 360
gaggtgcgcg?gcgtgctcga?ccgggtgctc?gacggcgagg?tgtgcgacgt?cgccgcggag 420
atcggcccgc?gtatccccgc?cgcggtcgtc?tgcgagatcc?tcggcgtgcc?cgccgaggac 480
caggacatgc?tcatcgacct?gaccaatcac?gcgttcggcg?gcgaggacga?gctgttcgac 540
gggatgacgc?cgcgtcaggc?gcacaccgag?atccttgtct?acttcgacga?actgatcagc 600
gctcgccgcg?cgaaccccgg?cgacgacctc?gtcagcacgt?tgctgtccga?cggcgaactc 660
tcgatcgacg?acgtggtgct?caactgcgac?aacgtgctga?tcggcggcaa?cgagaccacc 720
cggcacgcga?tcaccggcgc?ggtgcacgct?ttcgccgccg?tgccgggact?tctggaccgg 780
gtgcgggacg?gcgacgtcga?cgtcgacccc?gtcgtggacg?aggtgctgcg?ctggacctcg 840
cccgcgatgc?acgtgctacg?cgtgacgacc?ggcgaagtca?cgctcaacgg?ccgcgacctc 900
gaagccggta?cgccggtggt?cgcgtggctg?cccgccgcga?accgggatcc?cgccgtgttc 960
gaggaaccgg?acacgttccg?gccggggcgg?aaacccaacc?ggcacatcgc?tttcgggcac 1020
ggcatgcacc?actgcctcgg?ctccgcgctc?gcgcggatcg?agctggcggt?cgtgctgcgg 1080
gaggtggccg?gccgggtgtc?acgcgtggag?ctggagaagg?aaccggcctg?gctgcgcgcg 1140
atcgtcgtgc?aggggtaccg?ggaactcccg?gtgcggttca?ccggacgctg?a 1191
<210>18
<211>1035
<212>DNA
＜213〉east amycolatosis
<400>18
acgcagaatt?tcgagatcga?ctatgtggaa?atgtacgtcg?ccaacctcga?ggcggccgcg 60
tccggctgga?tggacaagta?cggcttctcc?gtcaccgcca?ccgaccggtc?ggccgaccac 120
cggagcgtga?cgctgcggca?cagcgcgatc?gcgctggtgc?tgaccgagcc?gctgtcggac 180
cggcaccccg?gggcgaccta?cctgcagacc?cacggtgacg?gggtcgccga?catcgcgctg 240
cgcacccccg?acgtggccgc?cgctttcgag?gccgcggtga?aggcgggcgc?ggaacccgtc 300
cgcgagccgg?agaagcgggc?ggactcggtc?gtcacggcca?ccgtcagagg?gttcggcgat 360
gtcgtgcaca?ccctgatcca?gagcgacgcc?gccgaggaga?ccccgcgcgg?caagggcggc 420
gtggagctgg?gcgtgatcga?ccacttcgcg?gtctgcctca?acgccggcga?cctcggcccc 480
acggtggcgt?tctacgagcg?cgcgctcggt?ttcaagcaga?tcttcgagga?gcacatcgtg 540
gtcggcgcgc?aggcgatgaa?ctccaccgtc?gtgcagagca?cgtcgggcgc?ggtcaccctc 600
accctgatcg?agccggacaa?gaccgccgac?cccggccaga?tcgacgactt?catcaaggag 660
caccacgggt?ccggcgtcca?gcacatcgcc?ttcaccagcc?cggacgcggt?ccgcgcggtc 720
aaggagcttt?ccgcgcgcgg?cgtggaattc?ctgaagaccc?cggacgccta?ctacgacctg 780
ctcggcgagc?ggatcgagct?ggagacgcac?tcgctggacg?atctgcgcga?gacgaagctg 840
ctcgccgacg?aggaccacgg?cggccagctg?ttccagatct?tcaccgcctc?cacgcatccg 900
cgtaagacga?tcttcttcga?gatcatcgag?cgccagggcg?ccggcacgtt?cggcagctcg 960
aacatcaagg?ccctgtacga?agccgtggag?ctggagcgca?ccgggcagag?caagctcggc?1020
cccgtccgtc?gatga 1035
<210>19
<211>1080
<212>DNA
＜213〉east amycolatosis
<400>19
atgacccacg?tctgtctcga?cgacctcgag?cgcgcggcac?gcgatgtcct?ctccggcgag 60
aactgggatt?tcctcgccgg?gggcagcggc?gcggaagcct?ccttggaggc?caaccgcgcc 120
gcgctcgagc?ggatcttcgt?gatcccccgg?gtattgcgtg?agctgaccgg?tggcaccgtc 180
gaggccgaac?tcctcggcgg?tcgcgccgcg?ctgccgctgg?cggtcgcgcc?cgtcgcgtac 240
cagcggttgt?tccaccccga?aggcgagctc?gccgcggcac?gcgccgcccg?ggacgcgggc 300
gtgccgtaca?ccatctgcac?gctgagcagt?gtcccgctgg?aggagatcgc?ggccgtcggc 360
ggacggccgt?ggttccagct?gtactggctg?cgggacgaga?aacgctcgct?cgacctcgtc 420
cgccgcgcgg?aggacgccgg?ctgcgaggcg?atcgtgttca?ccgtcgacgt?gccgtggatg 480
ggacgacggc?tgcgggacat?gcggaacggc?ttcgcgctgc?cggattccgt?gaccgcggcc 540
aatttcgacg?ccggggcggc?cgcgcaccac?cgcactcgcg?gccggtcggc?ggtggcggag 600
cacaccgcgc?gcgaattcgc?cccggccact?tgggaatccg?tcgaagcggt?ccgtgcgcac 660
acggatctcc?cggtggtgct?caaaggaatc?ctcgcggtcg?aggacgcgag?ccgcgccgtc 720
gacgcgggcg?ccgccgggat?cgtggtgtcc?aaccacggag?gcaggcagtt?ggacggcgcc 780
gtgccgggga?tcgagatgct?gggcgagatc?gccgccaccc?tttccggctg?gccgggcgaa 840
ctgctgctcg?acggcgggat?ccgcggtggc?ggcgacatcc?tcaaggcgat?cgcgctgggg 900
gcgtcgggtg?tcctggtcgg?acggcccgcc?atgtgggggc?tcgccgcggg?cggggaggac 960
ggcgtccggc?aggtgctcga?cctgctcgcc?gtcgaattcc?ggaacgcgct?gggcctcgcg?1020
ggctgcgact?cggtggacgc?ggcccggcgg?ctgggcacga?aggtctcccg?ccgcggctga?1080
<210>20
<211>1407
<212>DNA
＜213〉east amycolatosis
<400>20
atgacaagga?gtccttcgac?gctggtgcgc?ccccgcgacg?gccaggatca?cgcggaccgt 60
gtcgccttgt?ccgccgcgac?caccgatggc?gtgcacatgc?ggaccgccga?cgtccgcgcg 120
tggatcgccg?aacgccgtga?ggccaacgtc?ttccacgtcg?aacgcattcc?gttcgccgcg 180
ctggaccaat?ggggtttcga?ggacgggacc?ggcaacatcg?cgcaccggag?cggacggttc 240
ttcaccatcg?aagggctgca?cgtggtcgag?gccgacggcc?cgcacggcga?cggtccctac 300
cgcgaatggc?agcagcccgt?catcaaacag?gccgaagtcg?gcatcctcgg?catcctggcc 360
aaggagttcg?acggcgtcct?gcatttcctg?atgcaggcca?agatggagcc?ggggaatccc 420
aatctggtcc?agctgtctcc?gaccgtgcag?gccacgcgca?gcaattacac?caaggcgcac 480
ggcggcacga?acgtcaagct?gatcgagtac?ttcgcgccgc?ccgaccccga?gcgggtcatc 540
gtcgacgtgc?ttcaggcgga?gcagggatcg?tggttcctcc?agaagtccaa?tcgcaacatg 600
atcgtcgaga?ccgccgacga?agtgccgctg?tgggacgact?tctgctggct?caccctcggg 660
cagatcgcgg?agctgatgca?cgaggacgag?acgatcaaca?tgaacgccag?gagcgtgctg 720
tcgtgcctgc?cgtaccacga?caaggctccc?ggcgcgatgt?tctccgacgt?ccagctcctg 780
tcgtggttca?cgaacgagcg?gtcgcggcac?gacgtgcgcg?cccgccgcat?cccgctcaag 840
gacgtccgcg?actggaagcg?gggtgccgag?gcgatcgagc?acgaggacgg?ccgctacttc 900
agggtcctcg?cggtcgcggt?gaaaggcagc?aaccgcgaga?aggtcacctg?gacccagccg 960
ttgatcgaat?ccgtcgggat?gggcctcgtc?gcgttcctca?tgcgtgactt?cggcggcgtt?1020
ccgcacgtcc?tggcgcacgc?ccgcgccgac?ggcgggttcc?tggacacggt?cgagctggcg?1080
ccgaccgtcc?agtgcacgcc?gcagaactac?ctgcatctgc?ccgccgagca?acggccgccc?1140
tttctcgacc?tggtcctcgg?cgccccgcga?tcacggatcc?gttacgaggc?ggtacattcc?1200
gaagaaggcg?gccgcttcct?cagcgtccag?gcgcgttatc?tggcgatcga?ggcggacgac?1260
acggtcgagc?ctccgcccgg?ctacgcctgg?atcactccgg?ggcagctcac?cgcactcacc?1320
cggcacgggc?actacgtcaa?cgtcgaggcc?cgtacgctgc?tcgcctgcct?caacgccgcg?1380
gccgcgcagc?cacggggcgg?tgcctga 1407
<210>21
<211>972
<212>DNA
＜213〉east amycolatosis
<400>21
atgaagacga?tcaccgtcct?cggcgcgtcg?ggtttcgtcg?gctccgccgt?cgtgcgcgcg 60
ctggcgcggc?ggccggtccg?ggtgcgggcg?gtggcgcgca?ggcggctcac?gcccccgccc 120
ggccgggccg?agatcaccgc?cgtcgccgcc?gatctcaccg?accgcgccgc?gctcgccggc 180
gcggtcgccg?gctcggacgc?gatcgtgcac?ctgctgctcg?gtgacggcgg?ctggcgtggc 240
gcggagggcg?ccgaacgcgt?gaacgtggga?gtcctccggg?acctcgtcga?gatcgccggc 300
ggtggcgacg?ggccgcctcc?ggtggtggtg?ttcgccggaa?ccaccgcgca?ggtcggggtg 360
ccgccgcggg?aaccgctcga?cgggaccgaa?ccggaccacc?cggcgactcc?ttacgacgcc 420
cagaaactcg?aagcggaaca?actcctcaag?aaggccaccg?ccgacggcct?ggtgcgcggg 480
atcagcctgc?ggctgccgac?gatcttcggc?gagaccgcgg?ccgagggcgc?gaaccacgac 540
cgcggcgtgg?tgtccaccat?ggcgcggcgg?gcgctcgaag?gactaccgct?caccgtctgg 600
ggcgacggca?cggtgcgacg?cgacctggtg?cacgtcgacg?acgtcgcctc?ggcgttcatg 660
gccgcactgg?accatccgga?ttccctggtg?ggcgggcact?ggctgatcgg?tgccggtcga 720
ggggaccggc?tgggcgaggt?cttccggctc?gtcgcgaagg?agatgtccga?gcacacgggg 780
aaaagcccgg?tggaagtgtc?ctgtgtggag?cctccggcgc?acgcgcccga?gacggatctc 840
cggagcatca?ccatcgattc?cacgccgttc?cgtgcggtca?ccggctggcg?cccggagatc 900
tcgctgcccg?agggagtgcg?ccgcaccgtc?gccgctttga?cgtcatcagt?tcagggaagg 960
gttcacacat?ga 972
<210>22
<211>1110
<212>DNA
＜213〉east amycolatosis
<400>22
atgaccacgc?gcgtatggga?ctacctcgcc?gaatacggtg?ccgagcgggc?cgatctgctc 60
gacgcggtcg?agacggtctt?caactccggg?cagctcgtgc?tcggcgcgag?cctgcgcggc 120
ttcgagtcgg?aattcgccgc?gtaccacggc?gtcgggcact?gcgccggtct?cgacaacggg 180
acgaacgcga?tcaagctcgc?cctgcaggcg?ctgggtgtcg?ggccgggcga?cgaggtgatc 240
accgtgtcca?acaccgcggc?accgaccgtg?gtcgccatcg?acgggaccgg?ggccaccccg 300
gtcttcgtcg?acgtccgcga?ggaagacttc?ctgatggaca?ccggcctggt?cgaggccgcg 360
atcaccgaac?gcaccaaatg?cctgctgccc?gtgcacctgt?acggccagtg?cgtggacatg 420
gccccgctga?aggcgctcgc?cgcgaagcac?ggactgtccc?ttttggagga?ttgcgcccag 480
gcgcacggag?cccggcagaa?cgggacgatc?gcggggacgt?tcggcgacgc?ggccgcgttc 540
tccttctatc?ccaccaaggt?tctcggcgcg?tacggcgacg?gcggcgcggc?catcacctcc 600
gacgaagcgg?tggaccggcg?gctgcggcgg?ttgcgctact?acgggatgga?agagcgttac 660
tacacggtgg?aaacgcccgc?ccacaacagc?aggctggacg?aggtgcaggc?ggagatcctg 720
cggcgcaagc?tcaagcggct?cgacgcgtac?acggcgggcc?gccgggccgt?cgccgaacgc 780
tatgtcgagg?gcctcggcga?caccgacctg?aagcttccga?agaccacgcc?cggcaacgag 840
cacgtgtact?acgtgtacgt?ggtgcggcac?ccgcgacgcg?acgagatcat?cgagcggctc 900
aaggcgtacg?acatccacct?gaacatcagc?tacccgtggc?cggtgcacac?catgaccggg 960
ttcgcccacc?tcggctacga?gaagggctcc?ctgccggtca?ccgagaagct?ggccaccgag?1020
atcttctcgc?tgccgatgta?tcccgcgctt?tcccccgatc?tgcaggacaa?ggtcgtccac?1080
gccgtccgcg?aagtgctgtc?cagcctctga 1110
<210>23
<211>618
<212>DNA
＜213〉east amycolatosis
<400>23
gtgcaagcac?gcgaactggc?cgtcgaaggc?gcgatcgagt?tcacccccaa?ggtcttcccc 60
gacgatcgcg?gcctgttcct?ctcgccattc?caggaagagg?cgttcaccga?agcgcacggc 120
ggcccgctct?tccggacggt?gcagacgaat?cacagcagct?ccaaacgcgg?agtggtgcgg 180
ggcatccact?acaccacgac?gccgccgggc?accgcgaagt?acgtctactg?cgcgagcggt 240
caggcgctgg?acatcgtggt?cgacctccgg?gtcggctcgc?cgacgttcgg?gaaatgggac 300
gcggtcctgc?tggaccagcg?tgaccaccgc?gcgatgtact?tccccgtcgg?cgtcggccac 360
gccttcgtgg?cgctcgaaga?cgacaccgtc?atgtggtacc?tgctctccac?ggcctacgac 420
cccgggaacg?aactcgccct?ctcggtcctg?gatcccgcgc?tcggcctgcc?cgtcgacccc 480
ggcgtcgaac?cgatcctgtc?cgaacgcgac?caggtggcga?tcacgctcgc?cgaggcggaa 540
cgacagggac?tgctgccgga?ctacgccacc?tgcgtggaga?tcgaacggcg?actgtccgaa 600
gtgccccaga?ccacctga 618
<210>24
<211>1824
<212>DNA
＜213〉east amycolatosis
<400>24
gtgcaagcac?gcgaactggc?cgtcgaaggc?gcgatcgagt?tcacccccaa?ggtcttcccc 60
gacgatcgcg?gcctgttcct?ctcgccattc?caggaagagg?cgttcaccga?agcgcacggc 120
ggcccgctct?tccggacggt?gcagacgaat?cacagcagct?ccaaacgcgg?agtggtgcgg 180
ggcatccact?acaccacgac?gccgccgggc?accgcgaagt?acgtctactg?cgcgagcggt 240
caggcgctgg?acatcgtggt?cgacctccgg?gtcggctcgc?cgacgttcgg?gaaatgggac 300
gcggtcctgc?tggaccagcg?tgaccaccgc?gcgatgtact?tccccgtcgg?cgtcggccac 360
gccttcgtgg?cgctcgaaga?cgacaccgtc?atgtggtacc?tgctctccac?ggcctacgac 420
cccgggaacg?aactcgccct?ctcggtcctg?gatcccgcgc?tcggcctgcc?cgtcgacccc 480
ggcgtcgaac?cgatcctgtc?cgaacgcgac?caggtggcga?tcacgctcgc?cgaggcggaa 540
cgacagggac?tgctgccgga?ctacgccacc?tgcgtggaga?tcgaacggcg?actgtccgaa 600
gtgccccaga?ccacctgaaa?gcccactgtc?cactccagac?cagagaaagg?tgagatcgat 660
gcgcgctgcg?cggttcaagc?agctgctccg?gagcaaattg?agaacatggg?gatggatgta 720
tcgatgactg?ccctagccga?aaccaccgcg?gaccttgccg?tccacaatgg?attgaccgag 780
atcacccgat?tcgccggcgt?gggcaccgcg?gtgtccgagt?cgtcctactc?gcagtccgag 840
ctgctcgaaa?tcctcgacat?cgaggatccg?aagatccgct?cggtcttcct?gaacagcgcc 900
atcgatcggc?gtttcctcac?cctgccgccg?gagggtcccg?gcggagcccg?cgcggccgag 960
ccgcagggcg?acctcctcga?caagcacaag?aagatcgccg?tcgacatggg?ttgccgtgcc 1020
ctggaagcct?gcctgaagtc?ggcgggagcg?acgctttcgg?atctgcgtca?cctgtgctgt 1080
gtcacctcga?ccgggttcct?gacgcccggc?ctgagcgcgc?tcgtcatccg?ggaaatgggc 1140
atcgacccgc?attgcagccg?ctcggacatc?gtgggcatgg?gctgcaacgc?cggtctcaac 1200
gcgctcaacg?tggtcgcggg?ctggtccgcg?gcgcatcccg?gcgaactcgg?cgtcgtcctg 1260
tgcagcgagg?cgtgttccgc?cgcctacgcg?ctggacggca?cgatgcggac?cgcggtggtc 1320
aacagtcttt?tcggcgacgg?atccgcggcg?ctcgcggtcg?tctccggcga?cgggcgcgtg 1380
ccgggtccgc?gcgtcctgaa?attcgccagc?tacatcatca?ccgacgcggt?cgacgcgatg 1440
cgctacgact?gggaccgtga?ccaggaccgg?ttcagtttct?tcctcgaccc?gcagatcccc 1500
tacgtggtcg?gcgcgcacgc?cgaaatcgtc?atcgaccggc?ttctgtccgg?gacgggcctg 1560
cgccgcagcg?acatcggcca?ctggctggtg?cattcgggcg?ggaagaaggt?gatcgacgcc 1620
gtcgtcgtca?acctcggcct?gagccggtac?gacgtgcgcc?acaccaccgg?tgtgctccgc 1680
gactacggga?acctctccag?cggctccttc?ctcttctcct?acgagcggct?cgccgacgaa 1740
gacgtcgccc?gcccggggga?ctacggcgtg?ctcatgacca?tggggcccgg?ctccacgatc 1800
gaaatggcgc?ttatccaatg?gtga 1824
<210>25
<211>630
<212>DNA
＜213〉east amycolatosis
<400>25
atgctgcgct?tcgacggatc?ccgtccgctg?tccgccgcga?tcgtcgagga?gatcgacgcg 60
ctgtgcgatc?gagccgagga?cgagcgtgaa?cccggaccag?tcaccatcca?cgtcacgggc 120
gccccgcccg?agggctgggc?gaaggggctg?gcggtcgggc?tcgtgtccaa?atgggagcgg 180
gtcgtgcgcc?ggttcgaacg?cctcggcagg?ctcacggccg?cggtggcgtc?gggcgaatgc 240
gccggaacgg?ccctggacct?ccttctcgcc?gccgacatcc?ggatcgccga?accgggcacc 300
acgctgcggc?tcgcgtcggc?cggcggtggc?acctggccgg?gcatgaccgt?gtaccggctc 360
accaagcagg?ccggagcggc?gggcatccgc?cgggccgtac?tgctcggggc?gccactcggg 420
acggagcgcg?cgctcgccct?cgacctgatc?gacgaggtgt?ccgaagaccc?ggcgaagacg 480
ctggccgagc?tgaacgtcgt?catggacggc?gccgagacgg?cgatccggcg?gcagctgatc 540
ttcgaagcgg?gctcgaccac?cttcgaggag?gcgctcggtt?cccacctggc?cgccgcggac 600
cgggccctgc?ggcgggaagc?caagtcgtga 630
<210>26
<211>1227
<212>DNA
＜213〉east amycolatosis
<400>26
gtgacgacgg?atcaaaccgc?actttcgcca?gggctcgacc?tccgggccct?ggcgggggag 60
gcccaccggg?tcgacgacga?cgtccggacg?ctgcgtgcgg?ccttcgtcga?ggcgcacgcc 120
gaggagatct?acgccgaact?caccggcggc?cggacgcggt?acctgcggat?ggacgaactc 180
gtccgcgccg?cggcgctcgc?ctttccgggt?ctggtgccgt?cggaggagca?gatggcggcc 240
gagcgtgcac?ggccgcaggc?ggagaaggag?gggcgcgaga?tcgaccaggg?catcttcctg 300
cgcgggatcc?tgcgcgcgcc?gaaggcgggc?ccgcatctgc?tcgacgccat?gctccggccc 360
accgccaggg?cgcagcggct?gctcccggag?ttccttgaaa?ccggcgtcgt?gcagatggag 420
gccgtccggc?tggagcgccg?ggacggcgtc?gcgcacctga?ccctgtgccg?ggacgactgc 480
ctgaacgccg?aggacgccca?gcaggtcgac?gacatggaga?ccgcggtcga?cctggtgctg 540
ctcgacccgt?ccgtccgggt?gggactggtg?cggggcgggg?tgatgagcca?cccccgttac 600
ctggggcgcc?gggtgttctg?cgcggggatc?aacctcaaga?agctcagctc?cggcgacatc 660
ccgctggtgg?atttcctgct?gcggcgggaa?accgggtaca?tccagaagat?cttccacggt 720
ctgctgacgg?agaattcctg?gcattcgagg?ttcaccggca?aaccctgggt?ggcggccgtc 780
gattcgttcg?ccatcggcgg?cgggacccag?ttgctgctgg?tcttcgacca?cgtgctggcg 840
gcctccgacg?cgtacctcag?cctgcccgcg?gccaaggagg?ggatcatccc?cggcgtctcc 900
aacttccggc?tttcccggat?cgccgggccg?cgggtggccc?ggcaggtgat?cctcggcggc 960
cgcaagctcc?gggcggacga?gccggacgcc?cgctcgatcg?tcgacgaggt?cgtgcggccg?1020
gaggaggtgg?actcggccat?cgaccgcgcg?ctcgcccggc?tcgacgggga?agccgtcgcg?1080
gccaaccggc?gcatgctgaa?cctttccgag?gaaccaccgg?aggaattccg?ccggtatatc?1140
gccgaattcg?cgctgcagca?ggccctgcgc?atctacgggg?cggacgtgat?cggcaaggtg?1200
gacggcttcg?cggtggggtc?gcgatga 1227
<210>27
<211>792
<212>DNA
＜213〉east amycolatosis
<400>27
atgaacggcg?tccggtacga?gaagaaggac?cacgtcgcct?acgtgacgat?ggaccggccc 60
gaggtgctga?acgcgatgga?ccggcggatg?cacgcggaac?tcgccgggat?ctgggacgac 120
gtcgaagccg?acgacgacgt?cagggcggtg?gtcctgaccg?gggcggggga?tcgggcgttc 180
tccgtcgggc?aggacctcaa?ggaacgcgcg?cggctgaccg?aggaaggcgt?cgaggcgtcg 240
acgttcggca?gcgccggtca?gccgggccat?ccccggctga?ccgaccggtt?cacgctgtcc 300
aaaccggtgg?tcgcgcgtgt?gcacggctac?gcgctgggcg?gcggcttcga?actggtgctg 360
gcgtgcgaca?tcgtcgtcgc?ttccgaagaa?gcggtcttcg?ggttgccgga?ggtccgtctc 420
gggctgatcg?ccggggcggg?cggcgttttc?cggttgccgc?gccagcttcc?gcagaaagtg 480
gcgatgggtc?atctgctgac?cgggcgcaga?atggacgcgg?cgacggcact?gcgtcacggc 540
ctggtcaacg?aggtcgtccc?ggtcgcggaa?ttggaccggt?gcgtcgccga?atggacggac 600
gatctggtgc?gtgccgcgcc?gctttcggta?cgggcgatca?aagaggccgc?gctgaagtca 660
ctcgacctcc?cgctcgaaga?agcgttcaaa?acctcgtatc?cgtgggaaga?acggcggcgg 720
acgagcgcgg?acgcgagtga?gggcgcccgc?gccttcgccg?agaaacggga?cccgatctgg 780
accggtcgct?ga 792
<210>28
<211>21
<212>DNA
＜213〉east amycolatosis
<400>28
gccacggcga?ccggcccatc?c 21
<210>29
<211>27
<212>DNA
＜213〉east amycolatosis
<400>29
ctgaagcttc?cgcgatctgg?ggaacca 27
Claims (1)
1. a vancocin biological synthesis gene cluster is characterized in that, the nucleotide sequence of this gene cluster is shown in SEQ ID NO:1, and the gene of whole vancocin biological synthesis gene cluster is specially:
A, vancomycin precursor synthase gene: the vcm1 of coding prephenate dehydrogenase, the vcm17 of coding hydroxymandelic acid synthetic enzyme Hmas, the vcm18 of coding hydroxymandelic acid oxydase Hmo, the vcm13 of coding hydroxyphenylglycine transaminase HpgT, the vcm23 of coding dihydroxyphenyl acetic acid synthetic enzyme, the vcm24 of coding alkene acyl CoA lytic enzyme, the vcm25 of coding hydroxyacyl dehydrogenase, the vcm26 of coding alkene acyl CoA lytic enzyme, the vcm22 of coding epimerase, the vcm19 of coding NDP-hexose dehydrogenase, the vcm21 of coding C3-transaminase, the vcm11 of coding C3-methyltransgerase, the vcm20 of coding keto reductase, the vcm14 of coding lytic enzyme, the vcm15 of encoded peptide synthetic enzyme VCP4 and the oxidasic vcm16 of coding P450, totally 16 genes;
B, vancomycin synthase gene: the vcm2 of coding non-ribosomal peptide synthetase Vcp1, the vcm3 of coding non-ribosomal peptide synthetase Vcp2, the vcm4 of coding non-ribosomal peptide synthetase Vcp3, the vcm10 of the vcm9 of encoding glycosyl transferring enzyme GtfE and encoding glycosyl transferring enzyme GtfD, totally 5 genes;
C, vancomycin modification gene: the vcm12 of coding N-methyltransgerase, the vcm8 of coding chlB4, the vcm11 of coding C-methyltransgerase, the vcm5 of oxydase Oxy1, the vcm7 of the vcm6 of oxydase Oxy2 and oxydase Oxy3, totally 6 genes.
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| CN2007100368615A CN101063140B (en) | 2007-01-26 | 2007-01-26 | Vancocin biological synthesis gene cluster |
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| CN101724673B (en) * | 2008-10-29 | 2013-10-16 | 上海来益生物药物研究开发中心有限责任公司 | Method for preparing methyl vancomycin |
| CN102050869B (en) * | 2009-11-04 | 2016-05-18 | 上海来益生物药物研究开发中心有限责任公司 | A kind of vancomycin compound |
| CN108929860B (en) * | 2017-05-23 | 2023-08-22 | 上海来益生物药物研究开发中心有限责任公司 | Gene engineering bacterium for producing chloroeremomycin as well as preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1523034A (en) * | 2003-09-11 | 2004-08-25 | 上海交通大学 | Southern oligomycin biosynthetic gene cluster |
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| CN1523034A (en) * | 2003-09-11 | 2004-08-25 | 上海交通大学 | Southern oligomycin biosynthetic gene cluster |
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| Title |
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| 王耀耀等.组合链霉素和利福平抗性突变去甲基万古霉素高产菌株的选育.《中国抗生素杂志》.2006,第31卷(第4期),243. * |
| 陈代杰等.万古霉素的研究开发.《中国抗生素杂志》.2004,第29卷(第1期),8. * |
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Effective date of registration: 20200529 Address after: 200032 building 4, No. 300 Fenglin Road, Xuhui District, Shanghai Co-patentee after: Shanghai Health Creation Center for Biopharmaceutical R&D Co.,Ltd. Patentee after: Center for excellence and innovation in molecular plant science, Chinese Academy of Sciences Address before: 200031 No. 320, Yueyang Road, Shanghai, Xuhui District Co-patentee before: Shanghai Health Creation Center for Biopharmaceutical R&D Co.,Ltd. Patentee before: SHANGHAI INSTITUTES FOR BIOLOGICAL SCIENCES, CHINESE ACADEMY OF SCIENCES |
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