KR20050114099A - 대장암 진단용 ｄｎａ 칩 - Google Patents

대장암 진단용 ｄｎａ 칩 Download PDF

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KR20050114099A
KR20050114099A KR1020040039303A KR20040039303A KR20050114099A KR 20050114099 A KR20050114099 A KR 20050114099A KR 1020040039303 A KR1020040039303 A KR 1020040039303A KR 20040039303 A KR20040039303 A KR 20040039303A KR 20050114099 A KR20050114099 A KR 20050114099A
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dna
homo sapiens
colorectal cancer
dna chip
gene
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KR1020040039303A
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남명진
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남명진
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

Abstract

본 발명은 대장암 특이적인 유전자를 분석하기 위한 대장암 진단용 DNA 칩, 전기 DNA 칩의 제조방법 및 전기 DNA 칩을 포함하는 대장암 진단용 키트에 관한 것이다. 본 발명의 대장암 진단용 DNA 칩은 대장암 조직에서 특이적으로 발현되는 유전자와 결합할 수 있는 유전자 탐침; 올리고(dT)15, -(CH2)6- 및 아민(amine)기를 순차적으로 포함하고, 전기 유전자 탐침의 5' 말단이 올리고(dT)15의 3'말단부위에 연결된 링커; 및, 표면에 알데히드가 부착되고, 전기 링커의 아민기가 표면의 알데히드기와 시프염기 반응을 통해 연결된 고체기판을 포함한다. 본 발명의 대장암 특이적 DNA 칩 및 전기 DNA 칩을 포함하는 대장암 진단용 키트를 사용하면, 대장암 조직에서만 특이적으로 발현되는 유전자의 발현유무를 신속하게 확인할 수 있으므로, 대장암의 조기진단에 널리 활용될 수 있을 것이다.

Description

대장암 진단용 ＤＮＡ 칩{DNA Chip for Diagnosis of Colon Cancer}
본 발명은 대장암 진단용 DNA 칩에 관한 것이다. 좀 더 구체적으로, 본 발명은 대장암 특이적인 유전자를 분석하기 위한 대장암 진단용 DNA 칩 및 전기 DNA 칩을 포함하는 대장암 진단용 키트에 관한 것이다.
현대인의 주요 질환 중에서, 암의 치료방법과 진단방법에 관한 연구는 발병빈도가 높은 폐암, 간암, 위암 등을 중심으로 비교적 활발히 진행되고 있다. 그러나, 발병빈도가 낮은 식도암, 대장암 등에 대한 연구는 상대적으로 저조한 실정이다. 한편, 대장암은 초기에는 별로 증세를 느끼지 않기 때문에 정기적인 진단이 매우 중요하며, 특히, 40대 이상에서 소화 불량이나 배변 습관의 변화, 혈변, 동통 및 빈혈의 징후가 있으면, 반드시 대장암 검사를 받아보는 것이 바람직하다. 상기 대장암 검사는 직장수지 검사(digital rectal examination), 결장경검사(sigmoidoscopy) 대장내시경(colonoscopy) 또는 대장조영술(barium enema) 등의 형태로 수행되는데, 직장수지 검사는 검진자가 장갑을 착용하고 윤활제를 바른 다음, 직장에 손을 삽입하여 촉진을 통해 대장의 정상여부를 알아보는 방법이며, 결장경검사는 거울이 달린 길고 유연한 튜브를 직장을 통해 결장과 대장까지 삽입하여 직접 관찰하고, 대장내시경검사는 내시경을 이용하여 직접적으로 대장내부를 검사하는 방법이고, 대장조영술은 조영제를 투입한 후, 단층촬영하여, 대장에 분포된 혈관의 이상을 검진하는 방법이다. 이러한 검사방법은 대장의 이상유무를 단순히 판단하는 방법이며, 이들 방법에 의하여, 질병의 병변이 발견되는 경우, 보다 구체적으로 조직검사를 수행하게 된다. 질병의 병변이 발견되어 조직검사를 수행하여 진단하는 경우, 비교적 정확한 검사결과를 얻을 수도 있으나, 복합적인 원인에 의하여 발생하는 병변 또는 질환의 초기상태에서 나타나는 병변의 경우에는 부정확하거나 또는 잘못된 검사결과를 얻을 수도 있기 때문에, 이러한 오진율을 낮추려는 노력이 계속되고 있다.
현재, 암과 같이 유전적인 변이가 발생하는 질환에 대하여는 유전자를 이용하여 진단하는 방법이 개발되고 있는데, 발병빈도가 높은 폐암, 간암, 위암 등에 대하여는 상당한 성과가 보고되고 있으나, 상대적으로 발병빈도가 낮은 대장암의 경우에는 연구성과가 보고되지 않고 있는 실정이다.
따라서, 유전자를 이용하여 신속하고 정확하게 대장암을 진단할 수 있는 방법을 개발하여야 할 필요성이 끊임없이 대두되었다.
이에, 본 발명자들은 유전자를 이용하여 신속하고 정확하게 대장암을 진단할 수 있는 방법을 개발하고자 예의 연구 노력한 결과, 대장암 조직에서 특이적으로 발현되는 유전자와 결합할 수 있는 유전자 탐침을 포함하는 DNA 칩 및 시료에서 채취한 RNA로부터 합성된 cDNA를 표지하기 위하여 형광표지된 데옥시 뉴클레오타이드를 포함하는 대장암 진단용 키트를 사용할 경우, 신속하고 정확하게 대장암을 진단할 수 있음을 확인하고, 본 발명을 완성하게 되었다.
결국, 본 발명의 주된 목적은 대장암의 발병을 진단하기 위한 대장암 진단용 DNA 칩을 제공하는 것이다.
본 발명의 다른 목적은 전기 DNA 칩을 포함하는 대장암 진단용 키트를 제공하는 것이다.
본 발명의 대장암 진단용 DNA 칩은 대장암 조직에서 특이적으로 발현되는 유전자와 결합할 수 있는 유전자 탐침; 올리고(dT)15, -(CH2)6- 및 아민(amine)기를 순차적으로 포함하고, 전기 유전자 탐침의 5' 말단이 올리고(dT)15의 3'말단부위에 연결된 링커; 및, 표면에 알데히드가 부착되고, 전기 링커의 아민기가 표면의 알데히드기와 시프염기(Schiff's base) 반응을 통해 연결된 고체기판을 포함한다: 이때, 유전자 탐침이 특별히 제한되는 것은 아니나, 서열번호 1 내지 1069의 염기서열로 표시되는 올리고뉴클레오티드로부터 선택된 1개 이상의 올리고뉴클레오티드를 사용함이 바람직하다.
본 발명자들은 환자들에게 불쾌감을 유발하지 않으면서도 대장암을 보다 신속하게 진단할 수 있는 방법을 개발하기 위하여, 대장암 조직에서 특이적으로 발현되는 유전자를 선별하고자 하였다. 그 결과, 54종의 유전자가 대장암 조직에서 특이적으로 높은 발현비율로 발현되고, 뇌, 폐, 췌장, 난소 등의 정상조직에서는 현저히 낮은 발현비율로 발현됨을 확인하였다. 이에, 본 발명자들은 전기 54종의 유전자의 발현을 검색할 수 있는 탐침을 제조하였는데, 각 유전자당 약 20개의 탐침을 제조하고, 이를 이용하여 제조된 DNA 칩을 사용할 경우, 환자들에게 불쾌감을 유발하지 않으면서도 대장암을 보다 신속하게 진단할 수 있음을 확인하였다.
본 발명의 전기 대장암 진단용 DNA 칩의 제조방법은 대장암 조직에서 특이적으로 발현되는 유전자와 결합할 수 있는 유전자 탐침을 합성하는 공정; 올리고(dT)15, -(CH2)6- 및 아민(amine)기를 순차적으로 포함하는 링커를 합성하는 공정; 전기 합성된 유전자 탐침의 5' 말단을 전기 합성된 링커의 올리고(dT)15의 3'말단부위에 결합시키는 공정; 유전자 탐침이 결합된 링커를 알데히드가 결합된 고체의 표면에 시프염기(Schiff's base)반응을 통해 결합시키는 공정; 및, 반응되지 않고 잔존하는 알데히드를 환원시키는 공정을 포함한다: 이때, 고체는 특별히 제한되는 것은 아니나 유리인 것이 바람직하고, 알데히드의 환원조건은 특별히 이에 제한되는 것은 아니나 NaBH4 등의 환원제를 사용함이 바람직하다.
한편, 본 발명의 대장암 진단용 DNA 칩을 이용하여, 대장암 환자를 선별하기 위한 진단키트를 제작할 수 있다. 즉, 본 발명의 대장암 진단용 키트는 전기 대장암 진단용 DNA 칩 및 시료에서 채취한 RNA로부터 합성된 cDNA를 표지하기 위하여 형광표지된 데옥시 뉴클레오타이드를 포함한다: 이때, 증폭산물을 표지할 수 있는 형광표지된 데옥시 뉴클레오타이드가 특별히 제한되는 것은 아니나 텍사스 레드(Texas Red) dATP, 시아닌 3(Cyanine 3) dCTP, 시아닌 5(Cyanine 5) dGTP, 플루오레세인-12(fluorescein-12) dUTP, 바이오틴-11-CTP, 바이오틴-16-UTP 등을 사용함이 바람직하다.
본 발명의 대장암 특이적 DNA 칩 및 전기 DNA 칩을 포함하는 대장암 진단용 키트를 사용하면, 대장암 조직에서만 특이적으로 발현되는 유전자의 발현유무를 신속하게 확인할 수 있으므로, 대장암의 조기진단에 널리 활용될 수 있을 것이다.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 요지에 따라 본 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 당업계에서 통상의 지식을 가진 자에 있어서 자명할 것이다.
실시예 1: 대장암 특이 유전자의 선별
정상 뇌조직(BN) 6건, 뇌의 1기 성상세포종(astrocytoma) 조직(BA I) 19건, 정상 대장조직(CN) 8건, MSS(microsatelite stability) 대장암조직(CMSS) 38건, MSI(microsatelite instability) 대장암조직(CMSI) 13건, 정상폐조직(LN) 10건, 1기 폐암조직(L I) 37건, 정상 췌장조직(PN) 7건, 췌장암조직(PT) 8건 및 장액성 난소암조직(ovary cancer serous, OS) 24건의 시료를 각각 수득하고, Trizol(Life Technologies, USA)을 이용하여 전기 수득한 시료로부터 RNA를 추출하였으며, 컬럼(RNesay spin column, Qiagen, USA)을 사용하여 정제하여, 18S와 28S rRNA가 유사하게 발현되는 RNA 만을 선별하였다.
전기 선별된 5㎍의 RNA를 oligo(dT)24를 프라이머로 사용한 cDNA 합성키트(cDNA synthesis kit, Superscript Choice System; Life Technologies, USA)에 적용하여 cDNA를 합성한 후, 전기 합성된 cDNA를 주형으로 하여 T7 polymerase in vitro 전사 시스템(T7 Megascript kit, Ambion, USA)를 이용하여 cRNA를 합성하였다. 이때, 전기 cRNA는 바이오틴-11-CTP와 바이오틴-16-UTP(Enzo, USA)를 이용하여 표지하였다. 이어, 전기 표지된 cRNA 15㎍을 절편화용액(40mM Tris-acetate, pH 8.1, 100mM potassium acetate and 30mM magnesium acetate)으로 처리하고, 94℃에서 30분간 반응시켜서, 30 내지 50 염기의 크기가 되게 무작위적으로 절편화시켰다. 그런 다음, 청어 정자 DNA(Promega, USA) 0.1mg/ml와 아세틸화된 BSA(Life Technologies, USA) 500g/ml를 포함하는 혼성화반응용액(hybridization cocktail, 100mmol/L MES, 1mol/L NaCl, 20mmol/L EDTA, 0.01%(v/v) Tween 20) 300㎕를 94℃의 온도로 5분동안 가열한 후, 냉각시키고, 상온에서 16,000g로 원심분리하여 상층액을 수득하였다. 전기 수득한 혼성화반응용액과 전기 절편화된 cDNA 10㎍을 혼합하고, 7129개의 유전자를 확인할 수 있는 마이크로어레이(HuGeneFL Array, Affymetrix, USA)에 적용한 후, 45℃에서 16시간 동안 혼성화반응을 수행하였다.
반응이 종결된 후, 마이크로어레이를 세척하고, 스트렙타비딘 용액(streptavidin-phycoerythrin, Molecular Probes, USA)으로 염색한 다음, 6x SSPE 완충용액(sodium chloride, sodium phosphate, EDTA)으로 세척하였다. 이어, 항-스트렙타비딘 항체(biotinylated anti-streptavidin IgG)를 처리하고, 스트렙타비딘 용액으로 다시 염색한 후, 6X SSPE로 다시 세척하였다. 그런 다음, 전기 마이크로어레이를 스캐너(GeneArray Scanner, Affymetrix, USA)로 스캔한 후, 이미지분석기(GeneChip software, Affymetrix, USA)를 사용하여 결과를 측정하고, 분위수-표준화(quantile-normalization)를 통하여 분석하였다(참조: 표 1).
각종 조직에서의 특정 유전자 발현비율 측정결과
유전자 AC MO R BN BA I CN CMSS CMSI LN L I PN PT OS
U51096 1013 111 9.16 -19 16 111 1086 805 21 31 57 31 2
X05232 855 158 5.41 33 27 99 777 1076 -4 32 43 158 -4
U22376 1758 199 8.85 42 -9 -19 1956 1195 127 149 56 -31 199
U51095 2498 438 5.70 33 93 193 2474 2564 58 51 438 179 54
X14253 1103 236 4.68 58 65 85 1349 405 22 57 236 80 48
L21998 6963 1345 5.18 859 1037 245 6139 9306 893 1141 1345 1057 1292
U30246 1578 288 5.48 93 29 288 1374 2160 70 151 108 80 100
U33317 1381 405 3.41 171 149 405 1494 1058 59 134 76 31 127
U79725 2424 730 3.32 65 26 730 2622 1862 345 102 10 49 74
L02785 1293 337 3.84 68 67 337 1710 108 13 32 87 213 31
M10050 7363 2652 2.78 -91 -135 2652 9101 2416 -148 -81 -12 -68 -145
L41349 628 188 3.33 108 79 61 701 421 79 47 188 98 65
X83228 4316 626 6.89 -35 -27 626 4641 3389 -45 41 56 147 -35
M30496 1777 572 3.11 356 341 290 1954 1273 382 435 189 478 572
Z29067 796 278 2.86 178 278 165 914 459 192 187 129 203 186
L41939 851 292 2.91 180 189 40 917 664 272 185 201 64 292
U07969 5795 626 9.25 180 181 626 5962 5317 127 234 -10 383 115
U26726 1773 463 3.83 91 43 463 1882 1464 97 237 309 347 321
X12901 1374 205 6.72 -21 13 188 1578 791 -44 183 205 141 -26
M73489 1406 686 2.05 204 169 362 1553 987 106 172 686 168 138
M76180 2273 647 3.52 6 14 647 2615 1300 116 326 580 189 48
L25931 2044 702 2.91 166 571 386 2177 1665 618 702 341 483 664
U55206 2009 613 3.28 117 162 159 2311 1149 141 292 38 124 613
M77140 578 188 3.08 22 29 188 637 412 -24 29 41 48 9
M30703 1054 537 1.96 -26 64 102 1214 595 487 537 22 239 -16
M29540 22036 5652 3.90 -12 5 331 23943 16608 83 5652 85 4015 -45
M63967 432 198 2.18 42 58 145 459 355 60 168 171 198 176
U64197 1063 470 2.26 109 60 470 1115 913 123 401 178 378 276
X98311 927 361 2.57 185 173 72 988 754 81 223 249 361 157
M15796 2201 1175 1.87 85 261 556 2028 2694 369 641 123 398 1175
(다음 면에서 계속)
D13645 1039 512 2.03 104 114 97 1080 921 277 332 230 345 512
M85085 282 153 1.84 67 66 22 265 328 -19 112 -26 81 153
L25876 1340 712 1.88 273 160 145 1250 1596 107 404 288 665 712
J04102 2466 1036 2.38 88 153 369 2605 2072 1036 851 -142 306 76
L00058 2309 998 2.31 42 282 374 2465 1866 735 566 422 463 998
M14758 615 344 1.79 242 251 76 768 179 344 58 94 77 95
D63486 8118 4381 1.85 3349 4381 4114 7767 9115 3169 4242 2799 2499 3738
U41813 570 325 1.75 48 26 325 548 631 -18 14 63 16 -3
M36067 338 195 1.73 102 3 -19 338 339 22 35 -3 -3 195
D87682 588 340 1.73 167 107 102 627 476 82 175 -29 84 340
X59543 1558 918 1.70 141 444 239 1440 1894 504 538 151 605 918
Z48541 1227 453 2.71 453 295 46 1530 366 313 144 210 75 98
U27333 4958 2009 2.47 -178 -192 2009 4630 5891 309 1258 -6 1139 104
X53586 2342 1376 1.70 188 355 1376 2349 2322 792 555 729 1136 571
U19523 559 314 1.78 -22 -18 196 512 692 314 304 65 214 160
U09564 2552 1438 1.77 724 519 735 2661 2242 638 1209 842 1438 1406
M29536 7070 4360 1.62 1945 2264 3891 7747 5144 2602 3279 2738 3016 4360
X68314 14315 9168 1.56 454 343 9168 15115 12039 162 3248 437 3224 254
D87716 479 303 1.58 149 197 63 410 674 146 207 58 303 288
L23808 2965 1471 2.02 27 -23 536 2716 3674 30 1471 126 254 496
U78525 4288 2719 1.58 1148 1391 1566 4606 3383 1070 2528 1169 2719 2412
D50919 446 274 1.63 -49 163 274 424 510 237 253 17 235 274
X60486 1950 1282 1.52 476 317 648 1710 2631 618 603 390 545 1282
U24169 2148 1414 1.52 338 537 496 2265 1815 415 966 209 1084 1414
상기 표 1에서, AC는 대장암조직에서의 특정 유전자 발현비율의 평균값을 나타내고, MO는 다른 조직 중에서의 해당 유전자의 발현비율 중 최고치를 나타내며, R은 전기 AC를 MO로 나눈 비율을 의미한다.
또한, 상기 표 1에서, 유전자 U51096는 꼬리형 호메오상자 전사인자 2(caudal type homeo box transcription factor 2), X05232는 세포간질 금속성 단백질 분해효소 3(matrix metalloproteinase 3), U22376는 v-myb 조류 골수아세포증 바이러스성 암유전자 유사유전자(avian myeloblastosis viral oncogene homolog), U51095는 꼬리형 호메오상자 전사인자 1(caudal type homeo box transcription factor 1), X14253는 기형암종-유래 성장인자 1(teratocarcinoma-derived growth factor 1), L21998는 소장/기관 특이적 뮤신 2(intestinal/tracheal mucin 2), U30246는 나트륨/칼륨/염소 수송체(sodium/potassium/chloride transporter)인 용질 수송체 12군 멤버 2(solute carrier family 12), U33317은 파네스세포 특이적 데펜신 알파6(Paneth cell-specific defensin alpha 6), U79725는 막투과 당단백질 A33(trans-membrane glycoprotein A33), L02785는 용질 수송체 26군 멤버 3(solute carrier family 26, member 3), M10050은 지방산 결합단백질 1(fatty acid binding protein 1), L41349는 포스포리파제 C 베타 4(phospholipase C, beta 4), X83228은 간-소장 카드헤드린(liver-intestine cadhedrine)인 카드헤드린 17(cadherin 17), M30496은 유비퀴틴 티오에스테라제(ubiquitin thiolesterase)인 유비퀴틴 카르복실-말단 에스테라제 L3(ubiquitin carboxyl-terminal esterase L3), Z29067은 NIMA (never in mitosis gene a)-연관 키나제(NIMA-related kinase) 3, L41939는 EphB2, U07969는 간-소장 카드헤드린(liver-intestine cadhedrine)인 카드헤드린 17(cadherin 17), U26726는 하이드록시스테로이드(11-베타) 탈수소효소 2(hydroxysteroid (11-beta) dehydrogenase)2), X12901는 빌린 1(villin 1), M73489는 열안정성 엔테로톡신 수용체(heat stable enterotoxin receptor)인 구아닐레이트 환형화효소 2C(guanylate cyclase 2C), M76180은 방향성 L-아미노산 디카르복실화효소(aromatic L-amino acid decarboxylase)인 도파 탈카르복실화효소(dopa decarboxylase), L25931은 라민 B 수용체(lamin B receptor), U55206은 접합효소(conjugase)이자 폴리폴리감마글루타밀 가수분해효소(gamma-glutamyl hydrolase)인 감마-글루타밀 가수분해효소(gamma-glutamyl hydrolase), M77140은 갈라닌(galanin), M30703은 신경초종-유래 성장인자(schwannoma-derived growth factor)인 암피레귤린(amphiregulin), M29540은 암태아성 항원-연관 세포유착분자 5(carcinoembryonic antigen-related cell adhesion molecule 5), M63967은 알데히드 탈수소효소 5(aldehyde dehydrogenase 5), U64197은 소형 유도성 사이토카인 아과 A 멤버 20(small inducible cytokine subfamily, member 20), X98311은 암태아성 항원-연관 세포유착분자 7(carcinoembryonic antigen-related cell adhesion molecule 7), M15796은 증식세포 핵항원(proliferating cell nuclear antigen), D13645는 KIAA0020 유전자 산물, M85085는 64kD 크기의 분열 촉진인자 단위체 2(cleavage stimulation factor, subunit 2), L25876은 CDK2-연관 이중 특이성 탈인산화효소(CDK2-associated dual specificity phosphatase)인 싸이클린-의존성 키나제 억제제 3(cyclin-dependent kinase inhibitor 3), J04102는 v-ets 조류 적아세포증 바이러스 E26 암유전자 유사유전자 2(avian erythroblastosis virus E26 oncogene homolog 2), L00058은 v-myc 조류 골수세포종증 바이러스성 암유전자 유사유전자(avian myelocytomatosis viral oncogene homolog), M14758는 ATP-결합 카세트 아과 B 멤버 1(ATP-binding cassette, sub-family B, member 1), D63486은 KIAA0152 유전자 산물, U41813은 호메오 상자 A9(homeo box A9), M36067은 ATP-의존성 DNA 리가제 I(ATP-dependent DNA ligase I), D87682는 KIAA0241 단백질, X59543는 리보핵산 환원효소 M1(ribonucleotide reductase M1), Z48541는 수용체형(receptor type)인 단백질 티로신 탈인산화효소 O(protein tyrosine phosphatase O), U27333은 갈락토시드 3(4)-L-푸코실전이효소(galacto-side 3(4)-L-fucosyltransferase)인 푸코실전이효소 3(fucosyltransferase 3), X53586는 인테그린 알파 6(integrin alpha 6), U19523은 GTP 사이클로히드롤라제 1(GTP cyclohydrolase 1), U09564은 SFRS(splicing factor serine/arginine specific) 단백질 키나제 1(protein kianse 1), M29536은 38kD 크기의 진핵생물 번역개시인자 2 단위체 2(eukaryotic translation initiation factor 2, subunit 2), X68314은 글루타티온 과산화효소 2(glutathione peroxidase 2), D87716은 KIAA0007 단백질, L23808은 대식세포 엘라스타제(macrophage elastase)인 세포간질 금속성 단백질 분해효소 12(matrix metalloproteinase 12), U78525는 116kD 크기의 진핵생물 번역개시인자 3 단위체 9(eukaryotic translation initiation factor 3, subunit 9), D50919는 KIAA0129 유전자산물, X60486은 H4 히스톤 계열, 멤버 G(H4 histone family, member G) 및 U24169는 JTV1 유전자산물을 각각 의미한다.
전기 표 1에서 보듯이, 전기 유전자들은 대장암조직에서의 발현정도가 정상 대장조직은 물론 다른 암조직에서의 발현정도보다 월등히 높다는 것을 확인할 수 있었다.
실시예 2: DNA 칩의 제조
전기 실시예 1에서 확인된 54종의 유전자를 검출할 수 있는 복수개의 탐침을 각 유전자별로 제조하고, 제조된 탐침을 이용하여 DNA 칩을 제조하였다.
실시예 2-1: 탐침의 수득
전기 실시예 1에서 대장암 조직에서 특이적으로 발현되는 유전자로 확인된 54종의 유전자를 검출할 수 있는 복수개의 탐침을 합성하여 수득하였다(참조: 표 2).
대장암 조직에서 특이적으로 발현되는 유전자 검출용 탐침
유전자 서열번호 유전자 서열번호 유전자 서열번호
U51096 1-20 X12901 361-380 D63486 721-740
X05232 21-40 M73489 381-400 U41813 741-760
U22376 41-60 M76180 401-420 M36067 761-780
U51095 61-80 L25931 421-440 D87682 781-800
X14253 81-100 U55206 441-460 X59543 801-820
L21998 101-120 M77140 461-480 Z48541 821-840
U30246 121-140 M30703 481-500 U27333 841-849
U33317 141-160 M29540 501-520 X53586 850-869
U79725 161-180 M63967 521-540 U19523 870-889
L02785 181-200 U64197 541-560 U09564 890-909
M10050 201-220 X98311 561-580 M29536 910-929
L41349 221-240 M15796 581-600 X68314 930-949
X83228 241-260 D13645 601-620 D87716 950-969
M30496 261-280 M85085 621-640 L23808 970-989
Z29067 281-300 L25876 641-660 U78525 990-1009
L41939 301-320 J04102 661-680 D50919 1010-1029
U07969 321-340 L00058 681-700 X60486 1030-1049
U26726 341-360 M14758 701-720 U24169 1050-1069
실시예 2-2: DNA 칩의 제조
먼저, 올리고(dT)15, -(CH2)6- 및 아민(amine)기가 순차적으로 연결된 링커를 작제한 다음, 전기 실시예 2-1에서 수득한 각 탐침의 5' 말단을 링커의 티민부위에 연결시켰다.
이어, 전기 링커와 연결된 탐침을 완충용액(350mM sodium bicarbonate, pH 9.0)에 각각 50μM로 용해시키고, 알데히드가 부착된 슬라이드(silylated slide, CSS-100, CEL, USA) 표면에 어레이어(MicroGrid II, BioRobotics, USA)를 이용하여 크기 150㎛, 간격 400㎛로 점적한 후, 시프염기 반응을 수행하였다. 이어, 0.2%(w/v) SDS용액과 3차 증류수를 이용하여 순차적으로 세척하고, NaBH4 용액(0.1g NaBH4, 30㎖ PBS, 10㎖ ethanol)에 15분 동안 침지하여, 아민이 결합되지 않은 알데히드 잔기를 환원시킨 후, 3차증류수로 세척하고 건조시켜서 DNA 칩을 제조하였다.
실시예 3: DNA 칩을 이용한 대장암 환자의 선별
전기 실시예 2에서 제조된 DNA 칩을 이용하여, 대장암 환자를 진단할 수 있는지를 알아보기 위하여, 정상인, 대장암 환자 및 기타 암환자로부터 시료를 수득하고, 전기 시료를 전기 DNA 칩에 적용하여 대장암 환자를 선별할 수 있는지 확인하였다.
즉, 정상인 20명으로부터 수득한 대장조직 시료(대조군), 뇌의 1기 성상세포종 환자 20명으로부터 수득한 대장조직 시료(실험군 1), MSS 대장암 환자 20명으로부터 수득한 대장조직 시료(실험군 2), 1기 폐암 환자 20명으로부터 수득한 대장조직 시료(실험군 3), 췌장암 환자 20명으로부터 수득한 대장조직 시료(실험군 4) 및 난소암 환자 20명으로부터 수득한 대장조직 시료(실험군 5)로부터, RNA 추출키트(Micro-to-Midi Tatal RNA purification system, Life Technologies, USA)를 사용하여 각각의 mRNA를 분리하였고, 역전사 키트(Platinum Biochip Reagent Kit, Genocheck, 한국) 및 시아닌 5 dGTP(Cyanine 5 dGTP, Amersham, GB)를 이용하여 전기 분리된 mRNA로부터 형광표지된 각각의 cDNA를 합성하였다.
이어, 전기 실시예 2에서 제조된 DNA 칩에 전기 합성된 각 cDNA를 가하고, 혼성화반응을 12시간동안 수행한 후, 혼성화반응이 종결된 DNA 칩을 평판 스캐너(GenePix Scanner 4000A, Axon Inc, USA)으로 스캔하여, 전기 DNA 칩에 구비된 1069개의 탐침 중에서 형광발색반응을 나타내는 탐침의 수를 계수하고, 이로부터 발색반응 탐침의 평균비율을 측정하였다(참조: 표 3).
DNA 칩에 의하여 검출된 각 시료의 평균 발색반응 비율(%)
실험군 평균 발색반응 비율
대조군 5.3
실험군 1 10.6
실험군 2 73.4
실험군 3 12.6
실험군 4 9.7
실험군 5 11.4
상기 표 3에서 보듯이, 본 발명의 DNA 칩은 정상인의 대장조직 뿐만 아니라, 대장암이 아닌 다른 암환자에서 수득한 대장조직에서 수득한 시료에 대하여 낮은 발색반응 비율을 나타내는 반면, 대장암 환자의 대장조직에서 수득한 시료에 대하여는 매우 높은 발색반응 비율을 나타내므로, 대장암 환자의 식별에 용이하게 사용될 수 있음을 알 수 있었다.
실시예 4: DNA 칩을 이용한 대장암 환자의 선별(II)
전기 표 1에 도시된 유전자 중에서 대장암 조직에 상대적으로 높은 발현비율을 나타내는 9종의 유전자(U51096, X05232, U22376, U51095, L21998, U30246, X83228, U07969 및 X12901)로 부터 수득한 서열번호 1-20, 21-40, 41-60, 61-80, 101-120, 121-140, 241-260, 321-340 및 361-380의 180개의 탐침을 사용하는 것을 제외하고는, 전기 실시예 2-2의 방법으로 DNA 칩을 제조하였다.
이어, 전기 제조된 DNA 칩을 사용하는 것을 제외하고는, 실시예 3과 동일한 방법을 사용하여, 발색반응 탐침의 평균비율을 측정하였다(참조: 표 4).
DNA 칩에 의하여 검출된 각 시료의 평균 발색반응 비율(%)
실험군 평균 발색반응 비율
대조군 5.3
실험군 1 8.6
실험군 2 93.4
실험군 3 9.3
실험군 4 6.7
실험군 5 7.5
상기 표 4의 결과를 전기 표 3의 결과와 비교하면, 대장암 환자에 대한 검출감도가 현저히 증가하고, 대장암이 아닌 다른 암 환자에 대한 검출감도가 감소되었음을 알 수 있었는데, 이러한 결과는 전체 1069개의 탐침 중에서 대장암에 대한 특이성이 높은 탐침을 선별하여 사용하였기 때문에 나타난 결과라고 예측되었다.
이상에서 상세히 설명하고 입증하였듯이, 본 발명은 대장암 특이적인 유전자를 분석하기 위한 대장암 진단용 DNA 칩 및 전기 DNA 칩을 포함하는 대장암 진단용 키트를 제공한다. 본 발명의 대장암 특이적 DNA 칩 및 전기 DNA 칩을 포함하는 대장암 진단용 키트를 사용하면, 대장암 조직에서만 특이적으로 발현되는 유전자의 발현유무를 신속하게 확인할 수 있으므로, 대장암의 조기진단에 널리 활용될 수 있을 것이다.
이상으로 본 발명 내용의 특정한 부분을 상세히 기술하였는 바, 당업계의 통상의 지식을 가진 자에게 있어서, 이러한 구체적 기술은 단지 바람직한 실시태양일 뿐이며, 이에 의해 본 발명의 범위가 제한되는 것이 아닌 점은 명백할 것이다. 따라서, 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의하여 정의된다고 할 것이다.
<110> NAM, Myeong Jin <120> DNA Chip for Diagnosis of Colon Cancer <160> 1069 <170> KopatentIn 1.71 <210> 1 <211> 25 <212> DNA <213> Homo sapiens <400> 1 gacagaaagc ctccgctggg cttca 25 <210> 2 <211> 25 <212> DNA <213> Homo sapiens <400> 2 cattgcctgg actgaccaca ccaac 25 <210> 3 <211> 25 <212> DNA <213> Homo sapiens <400> 3 ccagcttcat ctatccgact cttct 25 <210> 4 <211> 25 <212> DNA <213> Homo sapiens <400> 4 ctcttctctt cctagatctg caggc 25 <210> 5 <211> 25 <212> DNA <213> Homo sapiens <400> 5 tcttcctaga tctgcaggct gcacc 25 <210> 6 <211> 25 <212> DNA <213> Homo sapiens <400> 6 tgcaggctgc acctctggct agagc 25 <210> 7 <211> 25 <212> DNA <213> Homo sapiens <400> 7 gaaaggcaag cttgaggcca agatg 25 <210> 8 <211> 25 <212> DNA <213> Homo sapiens <400> 8 ctcatggccc tcggaggtcc agctg 25 <210> 9 <211> 25 <212> DNA <213> Homo sapiens <400> 9 gccctcggag gtccagctgg gcctc 25 <210> 10 <211> 25 <212> DNA <213> Homo sapiens <400> 10 cctgcctccg ggcagcaagg tttac 25 <210> 11 <211> 25 <212> DNA <213> Homo sapiens <400> 11 ggtttacact gcggaacgca aaggc 25 <210> 12 <211> 25 <212> DNA <213> Homo sapiens <400> 12 cactgcggaa cgcaaaggca gctaa 25 <210> 13 <211> 25 <212> DNA <213> Homo sapiens <400> 13 ggaacgcaaa ggcagctaag ataga 25 <210> 14 <211> 25 <212> DNA <213> Homo sapiens <400> 14 ggactgacca aagactgcag aaccc 25 <210> 15 <211> 25 <212> DNA <213> Homo sapiens <400> 15 ccagaccagg aaaggcttgg ctggt 25 <210> 16 <211> 25 <212> DNA <213> Homo sapiens <400> 16 ccgaacagga cttgttagag agcct 25 <210> 17 <211> 25 <212> DNA <213> Homo sapiens <400> 17 aggacttgtt agagagcctg tcacc 25 <210> 18 <211> 25 <212> DNA <213> Homo sapiens <400> 18 cagagcttct ctgggctgaa tgtat 25 <210> 19 <211> 25 <212> DNA <213> Homo sapiens <400> 19 ttctctgggc tgaatgtatg tcagt 25 <210> 20 <211> 25 <212> DNA <213> Homo sapiens <400> 20 tgctataaat gccagagcca acctg 25 <210> 21 <211> 25 <212> DNA <213> Homo sapiens <400> 21 ttcacctaag tctctgtgaa ttgaa 25 <210> 22 <211> 25 <212> DNA <213> Homo sapiens <400> 22 taagtctctg tgaattgaaa tgttc 25 <210> 23 <211> 25 <212> DNA <213> Homo sapiens <400> 23 ctcctgcctg tgctgtgact cgagt 25 <210> 24 <211> 25 <212> DNA <213> Homo sapiens <400> 24 cctgtgctgt gactcgagtc acact 25 <210> 25 <211> 25 <212> DNA <213> Homo sapiens <400> 25 ctgtgactcg agtcacactc aaggg 25 <210> 26 <211> 25 <212> DNA <213> Homo sapiens <400> 26 tcaagggaac ttgagcgtga atctg 25 <210> 27 <211> 25 <212> DNA <213> Homo sapiens <400> 27 gagcgtgaat ctgtatcttg ccggt 25 <210> 28 <211> 25 <212> DNA <213> Homo sapiens <400> 28 tgccggtcat ttttatgtta ttaca 25 <210> 29 <211> 25 <212> DNA <213> Homo sapiens <400> 29 tcatttttat gttattacag ggcat 25 <210> 30 <211> 25 <212> DNA <213> Homo sapiens <400> 30 ttatgttatt acagggcatt caaat 25 <210> 31 <211> 25 <212> DNA <213> Homo sapiens <400> 31 agggcattca aatgggctgc tgctt 25 <210> 32 <211> 25 <212> DNA <213> Homo sapiens <400> 32 ttcaaatggg ctgctgctta gcttg 25 <210> 33 <211> 25 <212> DNA <213> Homo sapiens <400> 33 tgggctgctg cttagcttgc acctt 25 <210> 34 <211> 25 <212> DNA <213> Homo sapiens <400> 34 gctgcttagc ttgcaccttg tcaca 25 <210> 35 <211> 25 <212> DNA <213> Homo sapiens <400> 35 gcaccttgtc acatagagtg atctt 25 <210> 36 <211> 25 <212> DNA <213> Homo sapiens <400> 36 atagagtgat ctttcccaag agaag 25 <210> 37 <211> 25 <212> DNA <213> Homo sapiens <400> 37 ggggaagcac tcgtgtgcaa cagac 25 <210> 38 <211> 25 <212> DNA <213> Homo sapiens <400> 38 gcactcgtgt gcaacagaca agtga 25 <210> 39 <211> 25 <212> DNA <213> Homo sapiens <400> 39 caagtgactg tatctgtgta gacta 25 <210> 40 <211> 25 <212> DNA <213> Homo sapiens <400> 40 actgtatctg tgtagactat ttgct 25 <210> 41 <211> 25 <212> DNA <213> Homo sapiens <400> 41 atgaaaaacg ttttttgctg ctatg 25 <210> 42 <211> 25 <212> DNA <213> Homo sapiens <400> 42 tgctgctatg gtcttagcct gtaga 25 <210> 43 <211> 25 <212> DNA <213> Homo sapiens <400> 43 tagcctgtag acatgctgct agtat 25 <210> 44 <211> 25 <212> DNA <213> Homo sapiens <400> 44 agacatgctg ctagtatcag agggg 25 <210> 45 <211> 25 <212> DNA <213> Homo sapiens <400> 45 tagtatcaga ggggcagtag agctt 25 <210> 46 <211> 25 <212> DNA <213> Homo sapiens <400> 46 ttgactatgc actagtattt cagac 25 <210> 47 <211> 25 <212> DNA <213> Homo sapiens <400> 47 gcactagtat ttcagacttt ttaat 25 <210> 48 <211> 25 <212> DNA <213> Homo sapiens <400> 48 tacatttgaa aacttgtttg ggaga 25 <210> 49 <211> 25 <212> DNA <213> Homo sapiens <400> 49 gtttatacaa gcatgcgttg cactt 25 <210> 50 <211> 25 <212> DNA <213> Homo sapiens <400> 50 tataatttgg gagttctgca tttga 25 <210> 51 <211> 25 <212> DNA <213> Homo sapiens <400> 51 agttctgcat ttgatccgca tcccc 25 <210> 52 <211> 25 <212> DNA <213> Homo sapiens <400> 52 tatggtctca gaactgttgc atgga 25 <210> 53 <211> 25 <212> DNA <213> Homo sapiens <400> 53 gaactgttgc atggatcctg tgttt 25 <210> 54 <211> 25 <212> DNA <213> Homo sapiens <400> 54 gcatggatcc tgtgtttgca actgg 25 <210> 55 <211> 25 <212> DNA <213> Homo sapiens <400> 55 ctgtgtttgc aactggggag acaga 25 <210> 56 <211> 25 <212> DNA <213> Homo sapiens <400> 56 tgatagccag tcactgcctt aagaa 25 <210> 57 <211> 25 <212> DNA <213> Homo sapiens <400> 57 gtcactgcct taagaacatt tgatg 25 <210> 58 <211> 25 <212> DNA <213> Homo sapiens <400> 58 tgcaagatgg ccagcactga acttt 25 <210> 59 <211> 25 <212> DNA <213> Homo sapiens <400> 59 gccagcactg aacttttgag atatg 25 <210> 60 <211> 25 <212> DNA <213> Homo sapiens <400> 60 tgacggtgta cttactgcct tgtag 25 <210> 61 <211> 25 <212> DNA <213> Homo sapiens <400> 61 gtggggacca cactgatcct ggaga 25 <210> 62 <211> 25 <212> DNA <213> Homo sapiens <400> 62 accacactga tcctggagaa aaggg 25 <210> 63 <211> 25 <212> DNA <213> Homo sapiens <400> 63 ggaatgcttg cagagcatga cctga 25 <210> 64 <211> 25 <212> DNA <213> Homo sapiens <400> 64 cgtggtcaac tcacacctgc ctctt 25 <210> 65 <211> 25 <212> DNA <213> Homo sapiens <400> 65 tcccaggctg gatactaagc acaaa 25 <210> 66 <211> 25 <212> DNA <213> Homo sapiens <400> 66 tactaagcac aaagcccata gcact 25 <210> 67 <211> 25 <212> DNA <213> Homo sapiens <400> 67 gcacaaagcc catagcactg ggctc 25 <210> 68 <211> 25 <212> DNA <213> Homo sapiens <400> 68 agcccatagc actgggctct gatgg 25 <210> 69 <211> 25 <212> DNA <213> Homo sapiens <400> 69 ctgatggctg ctccactggg ttaca 25 <210> 70 <211> 25 <212> DNA <213> Homo sapiens <400> 70 gctgctccac tgggttacag aatca 25 <210> 71 <211> 25 <212> DNA <213> Homo sapiens <400> 71 ggttacagaa tcacagccct catga 25 <210> 72 <211> 25 <212> DNA <213> Homo sapiens <400> 72 ctcatgatca ttctcagtga gggct 25 <210> 73 <211> 25 <212> DNA <213> Homo sapiens <400> 73 atcattctca gtgagggctc tggat 25 <210> 74 <211> 25 <212> DNA <213> Homo sapiens <400> 74 ctcagtgagg gctctggatt gagag 25 <210> 75 <211> 25 <212> DNA <213> Homo sapiens <400> 75 ccaggctgcc catcagggcc caggg 25 <210> 76 <211> 25 <212> DNA <213> Homo sapiens <400> 76 gactttattc ggaccaagca gagct 25 <210> 77 <211> 25 <212> DNA <213> Homo sapiens <400> 77 accaagcaga gctcacagct ggaca 25 <210> 78 <211> 25 <212> DNA <213> Homo sapiens <400> 78 tcacagctgg acaggtgttg tatat 25 <210> 79 <211> 25 <212> DNA <213> Homo sapiens <400> 79 ggaatctctt ggatgcagct tcaag 25 <210> 80 <211> 25 <212> DNA <213> Homo sapiens <400> 80 tcttggatgc agcttcaaga ataaa 25 <210> 81 <211> 25 <212> DNA <213> Homo sapiens <400> 81 taatatgttc ttttaaatga ttgaa 25 <210> 82 <211> 25 <212> DNA <213> Homo sapiens <400> 82 atgtggacct tagaatacag ttttg 25 <210> 83 <211> 25 <212> DNA <213> Homo sapiens <400> 83 ggaaccagag tgctgaagga atgga 25 <210> 84 <211> 25 <212> DNA <213> Homo sapiens <400> 84 ggaatggaag tccatctgcg tgtgt 25 <210> 85 <211> 25 <212> DNA <213> Homo sapiens <400> 85 ccatctgcgt gtgtgcaggg agact 25 <210> 86 <211> 25 <212> DNA <213> Homo sapiens <400> 86 gttacttgat tggtgattag gtggt 25 <210> 87 <211> 25 <212> DNA <213> Homo sapiens <400> 87 gggcaagttt ccatcatcta tagaa 25 <210> 88 <211> 25 <212> DNA <213> Homo sapiens <400> 88 gtttccatca tctatagaaa gctat 25 <210> 89 <211> 25 <212> DNA <213> Homo sapiens <400> 89 attatacctc aatgtcccca acaag 25 <210> 90 <211> 25 <212> DNA <213> Homo sapiens <400> 90 tacctcaatg tccccaacaa gattg 25 <210> 91 <211> 25 <212> DNA <213> Homo sapiens <400> 91 tcaatgtccc caacaagatt gctta 25 <210> 92 <211> 25 <212> DNA <213> Homo sapiens <400> 92 tgtccccaac aagattgctt aataa 25 <210> 93 <211> 25 <212> DNA <213> Homo sapiens <400> 93 attgtgtttc ctccaagcta ttcaa 25 <210> 94 <211> 25 <212> DNA <213> Homo sapiens <400> 94 gtttcctcca agctattcaa ttctt 25 <210> 95 <211> 25 <212> DNA <213> Homo sapiens <400> 95 cctccaagct attcaattct tttaa 25 <210> 96 <211> 25 <212> DNA <213> Homo sapiens <400> 96 caagctattc aattctttta actgt 25 <210> 97 <211> 25 <212> DNA <213> Homo sapiens <400> 97 taaaccaagt gatcaaacta catat 25 <210> 98 <211> 25 <212> DNA <213> Homo sapiens <400> 98 ccaagtgatc aaactacata ttgta 25 <210> 99 <211> 25 <212> DNA <213> Homo sapiens <400> 99 tcaaactaca tattgtaaag cccat 25 <210> 100 <211> 25 <212> DNA <213> Homo sapiens <400> 100 tatatatgtg tatgcacagt aaaaa 25 <210> 101 <211> 25 <212> DNA <213> Homo sapiens <400> 101 aggtttcgta cgccggctgc accaa 25 <210> 102 <211> 25 <212> DNA <213> Homo sapiens <400> 102 cgtacgccgg ctgcaccaag accgt 25 <210> 103 <211> 25 <212> DNA <213> Homo sapiens <400> 103 agaccgtcct catgaatcat tgctc 25 <210> 104 <211> 25 <212> DNA <213> Homo sapiens <400> 104 tcctcatgaa tcattgctcc gggtc 25 <210> 105 <211> 25 <212> DNA <213> Homo sapiens <400> 105 attgctccgg gtcctgcggg acatt 25 <210> 106 <211> 25 <212> DNA <213> Homo sapiens <400> 106 cctgcgggac atttgtcatg tactc 25 <210> 107 <211> 25 <212> DNA <213> Homo sapiens <400> 107 ttgtcatgta ctcggccaag gccca 25 <210> 108 <211> 25 <212> DNA <213> Homo sapiens <400> 108 tgtactcggc caaggcccag gccct 25 <210> 109 <211> 25 <212> DNA <213> Homo sapiens <400> 109 aggcccaggc cctggaccac agctg 25 <210> 110 <211> 25 <212> DNA <213> Homo sapiens <400> 110 aggccctgga ccacagctgc tcctg 25 <210> 111 <211> 25 <212> DNA <213> Homo sapiens <400> 111 tggaccacag ctgctcctgc tgcaa 25 <210> 112 <211> 25 <212> DNA <213> Homo sapiens <400> 112 acagctgctc ctgctgcaaa gagga 25 <210> 113 <211> 25 <212> DNA <213> Homo sapiens <400> 113 aggtggtcct gagctgcccc aatgg 25 <210> 114 <211> 25 <212> DNA <213> Homo sapiens <400> 114 tcctgagctg ccccaatggc ggctc 25 <210> 115 <211> 25 <212> DNA <213> Homo sapiens <400> 115 cccacatcga gagctgccag tgcca 25 <210> 116 <211> 25 <212> DNA <213> Homo sapiens <400> 116 agtgccagga caccgtctgc gggct 25 <210> 117 <211> 25 <212> DNA <213> Homo sapiens <400> 117 taagctcggc ttcctctctt cagat 25 <210> 118 <211> 25 <212> DNA <213> Homo sapiens <400> 118 cggcttcctc tcttcagata tttat 25 <210> 119 <211> 25 <212> DNA <213> Homo sapiens <400> 119 gagtctttgt tcagtccttg ctttc 25 <210> 120 <211> 25 <212> DNA <213> Homo sapiens <400> 120 ttgttcagtc cttgctttcc aataa 25 <210> 121 <211> 25 <212> DNA <213> Homo sapiens <400> 121 gacataccgg cagatcaggt taaat 25 <210> 122 <211> 25 <212> DNA <213> Homo sapiens <400> 122 tattgtcatg agtctcccag ttgca 25 <210> 123 <211> 25 <212> DNA <213> Homo sapiens <400> 123 catgagtctc ccagttgcac gaaaa 25 <210> 124 <211> 25 <212> DNA <213> Homo sapiens <400> 124 agttgcacga aaaggtgctg tgtct 25 <210> 125 <211> 25 <212> DNA <213> Homo sapiens <400> 125 tgtgtctagt gctctctaca tggca 25 <210> 126 <211> 25 <212> DNA <213> Homo sapiens <400> 126 tctctacatg gcatggttag aagct 25 <210> 127 <211> 25 <212> DNA <213> Homo sapiens <400> 127 ttcataaatg ttctatacag tggac 25 <210> 128 <211> 25 <212> DNA <213> Homo sapiens <400> 128 aatgttctat acagtggaca gccct 25 <210> 129 <211> 25 <212> DNA <213> Homo sapiens <400> 129 ctatacagtg gacagccctc cagaa 25 <210> 130 <211> 25 <212> DNA <213> Homo sapiens <400> 130 tccagaatgg tacttcagtg cctag 25 <210> 131 <211> 25 <212> DNA <213> Homo sapiens <400> 131 atggtacttc agtgcctagt gtagt 25 <210> 132 <211> 25 <212> DNA <213> Homo sapiens <400> 132 tgcctagtgt agtaacctga aatct 25 <210> 133 <211> 25 <212> DNA <213> Homo sapiens <400> 133 gacacattaa catcacaatg gcgaa 25 <210> 134 <211> 25 <212> DNA <213> Homo sapiens <400> 134 tttgaaagca cacaggaaag cttgc 25 <210> 135 <211> 25 <212> DNA <213> Homo sapiens <400> 135 agcacacagg aaagcttgct ccatt 25 <210> 136 <211> 25 <212> DNA <213> Homo sapiens <400> 136 ctccattgat aacgtgtatg gagac 25 <210> 137 <211> 25 <212> DNA <213> Homo sapiens <400> 137 tcaattccat atctcaatct taatg 25 <210> 138 <211> 25 <212> DNA <213> Homo sapiens <400> 138 ccatatctca atcttaatgg tgatt 25 <210> 139 <211> 25 <212> DNA <213> Homo sapiens <400> 139 ggtgattctt ctctgttgaa ctgaa 25 <210> 140 <211> 25 <212> DNA <213> Homo sapiens <400> 140 tcttctctgt tgaactgaag tttgt 25 <210> 141 <211> 25 <212> DNA <213> Homo sapiens <400> 141 cactccaagc tgaggatgat ccact 25 <210> 142 <211> 25 <212> DNA <213> Homo sapiens <400> 142 aagctgagga tgatccactg caggc 25 <210> 143 <211> 25 <212> DNA <213> Homo sapiens <400> 143 cttatgaggc tgatgcccag gagca 25 <210> 144 <211> 25 <212> DNA <213> Homo sapiens <400> 144 atgaccagga ctttgccgtc tcctt 25 <210> 145 <211> 25 <212> DNA <213> Homo sapiens <400> 145 aggatgcaag ctcaagtctt agagc 25 <210> 146 <211> 25 <212> DNA <213> Homo sapiens <400> 146 ttagagcttt gggtggctca acaag 25 <210> 147 <211> 25 <212> DNA <213> Homo sapiens <400> 147 ctttgggtgg ctcaacaagg gcttt 25 <210> 148 <211> 25 <212> DNA <213> Homo sapiens <400> 148 caacaagggc tttcacttgc cattg 25 <210> 149 <211> 25 <212> DNA <213> Homo sapiens <400> 149 gccattgcag aaggtcctgt tattc 25 <210> 150 <211> 25 <212> DNA <213> Homo sapiens <400> 150 gcagaaggtc ctgttattca acaga 25 <210> 151 <211> 25 <212> DNA <213> Homo sapiens <400> 151 ggtcctgtta ttcaacagaa tattc 25 <210> 152 <211> 25 <212> DNA <213> Homo sapiens <400> 152 aatattccta tgggacctgc actgt 25 <210> 153 <211> 25 <212> DNA <213> Homo sapiens <400> 153 ggacctgcac tgtcatgggt attaa 25 <210> 154 <211> 25 <212> DNA <213> Homo sapiens <400> 154 accacagatt ctgctgcctc tgagg 25 <210> 155 <211> 25 <212> DNA <213> Homo sapiens <400> 155 ctccagcgac cctagccatg agaac 25 <210> 156 <211> 25 <212> DNA <213> Homo sapiens <400> 156 tcataattta ctttatgacc tagaa 25 <210> 157 <211> 25 <212> DNA <213> Homo sapiens <400> 157 ctgtcgtgtg tcctatacat tgcca 25 <210> 158 <211> 25 <212> DNA <213> Homo sapiens <400> 158 tgtgtcctat acattgccat caact 25 <210> 159 <211> 25 <212> DNA <213> Homo sapiens <400> 159 ctatacattg ccatcaactt tgttt 25 <210> 160 <211> 25 <212> DNA <213> Homo sapiens <400> 160 attgccatca actttgtttc ctcat 25 <210> 161 <211> 25 <212> DNA <213> Homo sapiens <400> 161 ttctctggtc tccttgagat gatcg 25 <210> 162 <211> 25 <212> DNA <213> Homo sapiens <400> 162 atgatcgtag acacagggat gattc 25 <210> 163 <211> 25 <212> DNA <213> Homo sapiens <400> 163 taaagtgaac acacacaagg gagct 25 <210> 164 <211> 25 <212> DNA <213> Homo sapiens <400> 164 acacaaggga gcttgcttgc agatg 25 <210> 165 <211> 25 <212> DNA <213> Homo sapiens <400> 165 ttgcttgcag atggtctgag ttctt 25 <210> 166 <211> 25 <212> DNA <213> Homo sapiens <400> 166 gcagatggtc tgagttcttg tgtcc 25 <210> 167 <211> 25 <212> DNA <213> Homo sapiens <400> 167 ataattggca tgtctcctca accca 25 <210> 168 <211> 25 <212> DNA <213> Homo sapiens <400> 168 tggttgttcc tgcatcccga tacct 25 <210> 169 <211> 25 <212> DNA <213> Homo sapiens <400> 169 cacagcctcc ttctgtttgt cgagc 25 <210> 170 <211> 25 <212> DNA <213> Homo sapiens <400> 170 gtcgagcatt tcttctactc ttgag 25 <210> 171 <211> 25 <212> DNA <213> Homo sapiens <400> 171 catttcttct actcttgaga gctca 25 <210> 172 <211> 25 <212> DNA <213> Homo sapiens <400> 172 ttctactctt gagagctcag gcagc 25 <210> 173 <211> 25 <212> DNA <213> Homo sapiens <400> 173 ggctgcttag gtctcatgga ccagt 25 <210> 174 <211> 25 <212> DNA <213> Homo sapiens <400> 174 ttaggtctca tggaccagtg gctgg 25 <210> 175 <211> 25 <212> DNA <213> Homo sapiens <400> 175 gaccagtggc tggtctcacc caact 25 <210> 176 <211> 25 <212> DNA <213> Homo sapiens <400> 176 tggctggtct cacccaactg cagtt 25 <210> 177 <211> 25 <212> DNA <213> Homo sapiens <400> 177 gtctcaccca actgcagttt actat 25 <210> 178 <211> 25 <212> DNA <213> Homo sapiens <400> 178 cccaactgca gtttactatt gctat 25 <210> 179 <211> 25 <212> DNA <213> Homo sapiens <400> 179 ttgctatctt ttctggatga tcaga 25 <210> 180 <211> 25 <212> DNA <213> Homo sapiens <400> 180 atcctttgct tcattccaaa tgctt 25 <210> 181 <211> 25 <212> DNA <213> Homo sapiens <400> 181 ttaccataaa tacaaatgga ggatt 25 <210> 182 <211> 25 <212> DNA <213> Homo sapiens <400> 182 tacgtaatcg ggtatatgag gtgcc 25 <210> 183 <211> 25 <212> DNA <213> Homo sapiens <400> 183 tatatgaggt gccagttgaa acaaa 25 <210> 184 <211> 25 <212> DNA <213> Homo sapiens <400> 184 cttcatctga ctatgacata aaaac 25 <210> 185 <211> 25 <212> DNA <213> Homo sapiens <400> 185 caactttata cccagaaagt tattg 25 <210> 186 <211> 25 <212> DNA <213> Homo sapiens <400> 186 tatacccaga aagttattga taagt 25 <210> 187 <211> 25 <212> DNA <213> Homo sapiens <400> 187 agaatatgtt tcaaactttg gaaca 25 <210> 188 <211> 25 <212> DNA <213> Homo sapiens <400> 188 tgtttcaaac tttggaacaa gatgg 25 <210> 189 <211> 25 <212> DNA <213> Homo sapiens <400> 189 gcatggcata tttttcacat atcta 25 <210> 190 <211> 25 <212> DNA <213> Homo sapiens <400> 190 catatttttc acatatctag tatga 25 <210> 191 <211> 25 <212> DNA <213> Homo sapiens <400> 191 tatatcttgt agctttatca aaggg 25 <210> 192 <211> 25 <212> DNA <213> Homo sapiens <400> 192 ttgttcatac atatttttgt agcac 25 <210> 193 <211> 25 <212> DNA <213> Homo sapiens <400> 193 atacatattt ttgtagcact gacag 25 <210> 194 <211> 25 <212> DNA <213> Homo sapiens <400> 194 gtagcactga cagatttcca tccta 25 <210> 195 <211> 25 <212> DNA <213> Homo sapiens <400> 195 ctgacagatt tccatcctag tcact 25 <210> 196 <211> 25 <212> DNA <213> Homo sapiens <400> 196 gatttccatc ctagtcacta ccttc 25 <210> 197 <211> 25 <212> DNA <213> Homo sapiens <400> 197 catcctagtc actaccttca tgcat 25 <210> 198 <211> 25 <212> DNA <213> Homo sapiens <400> 198 taccttcatg cataggttta gcagt 25 <210> 199 <211> 25 <212> DNA <213> Homo sapiens <400> 199 tagcagtata gtggcgccac tgttt 25 <210> 200 <211> 25 <212> DNA <213> Homo sapiens <400> 200 ggcgccactg ttttgaatct cataa 25 <210> 201 <211> 25 <212> DNA <213> Homo sapiens <400> 201 ggtctgccgg aagagctcat ccaga 25 <210> 202 <211> 25 <212> DNA <213> Homo sapiens <400> 202 ccggaagagc tcatccagaa gggga 25 <210> 203 <211> 25 <212> DNA <213> Homo sapiens <400> 203 gggtccaaag tgatccaaaa cgaat 25 <210> 204 <211> 25 <212> DNA <213> Homo sapiens <400> 204 gtcaagacag tggttcagtt ggaag 25 <210> 205 <211> 25 <212> DNA <213> Homo sapiens <400> 205 acagtggttc agttggaagg tgaca 25 <210> 206 <211> 25 <212> DNA <213> Homo sapiens <400> 206 acagctttca aaaacatcaa gtctg 25 <210> 207 <211> 25 <212> DNA <213> Homo sapiens <400> 207 gtgaccgaac tcaacggcga cataa 25 <210> 208 <211> 25 <212> DNA <213> Homo sapiens <400> 208 gaactcaacg gcgacataat cacca 25 <210> 209 <211> 25 <212> DNA <213> Homo sapiens <400> 209 gacataatca ccaataccat gacat 25 <210> 210 <211> 25 <212> DNA <213> Homo sapiens <400> 210 atcaccaata ccatgacatt gggtg 25 <210> 211 <211> 25 <212> DNA <213> Homo sapiens <400> 211 gggagctcta ttgccaccat gagtt 25 <210> 212 <211> 25 <212> DNA <213> Homo sapiens <400> 212 aataccatga cattgggtga cattg 25 <210> 213 <211> 25 <212> DNA <213> Homo sapiens <400> 213 atgacattgg gtgacattgt cttca 25 <210> 214 <211> 25 <212> DNA <213> Homo sapiens <400> 214 gacattgtct tcaagagaat cagca 25 <210> 215 <211> 25 <212> DNA <213> Homo sapiens <400> 215 atttaaacaa gtctgcattt catat 25 <210> 216 <211> 25 <212> DNA <213> Homo sapiens <400> 216 taaacaagtc tgcatttcat attat 25 <210> 217 <211> 25 <212> DNA <213> Homo sapiens <400> 217 tctattgcca ccatgagttt ctccg 25 <210> 218 <211> 25 <212> DNA <213> Homo sapiens <400> 218 ggcaagtacc aactgcagag ccagg 25 <210> 219 <211> 25 <212> DNA <213> Homo sapiens <400> 219 taccaactgc agagccagga aaact 25 <210> 220 <211> 25 <212> DNA <213> Homo sapiens <400> 220 tttgaagcct tcatgaaggc aatcg 25 <210> 221 <211> 25 <212> DNA <213> Homo sapiens <400> 221 agatgcagca gatggtgaaa ttgga 25 <210> 222 <211> 25 <212> DNA <213> Homo sapiens <400> 222 accgcagacc agcaacagta gtatg 25 <210> 223 <211> 25 <212> DNA <213> Homo sapiens <400> 223 tagtatgaaa ctccaaaatg caaac 25 <210> 224 <211> 25 <212> DNA <213> Homo sapiens <400> 224 acccacaaag catcaaaaga ctcac 25 <210> 225 <211> 25 <212> DNA <213> Homo sapiens <400> 225 gactcactca caaaacttct gaaca 25 <210> 226 <211> 25 <212> DNA <213> Homo sapiens <400> 226 ctcacaaaac ttctgaacac aaact 25 <210> 227 <211> 25 <212> DNA <213> Homo sapiens <400> 227 aaacttctga acacaaactc catgg 25 <210> 228 <211> 25 <212> DNA <213> Homo sapiens <400> 228 ctgaacacaa actccatgga tgaaa 25 <210> 229 <211> 25 <212> DNA <213> Homo sapiens <400> 229 agacttggaa tgtctgactg acttc 25 <210> 230 <211> 25 <212> DNA <213> Homo sapiens <400> 230 tctgactgac ttctatttaa cagct 25 <210> 231 <211> 25 <212> DNA <213> Homo sapiens <400> 231 aacagcttga gtattgcatt tcctt 25 <210> 232 <211> 25 <212> DNA <213> Homo sapiens <400> 232 ttgagtattg catttccttg gccaa 25 <210> 233 <211> 25 <212> DNA <213> Homo sapiens <400> 233 tactattcca gtaaggcaga gtcca 25 <210> 234 <211> 25 <212> DNA <213> Homo sapiens <400> 234 tccagtaagg cagagtccaa ccatt 25 <210> 235 <211> 25 <212> DNA <213> Homo sapiens <400> 235 aaggcagagt ccaaccattg ataat 25 <210> 236 <211> 25 <212> DNA <213> Homo sapiens <400> 236 tgataataca acttaaacat gtttg 25 <210> 237 <211> 25 <212> DNA <213> Homo sapiens <400> 237 tacaacttaa acatgtttgc tataa 25 <210> 238 <211> 25 <212> DNA <213> Homo sapiens <400> 238 aagtaaatga gcttggtgtg aacaa 25 <210> 239 <211> 25 <212> DNA <213> Homo sapiens <400> 239 tgaacaactc ttcctttgtg atgcc 25 <210> 240 <211> 25 <212> DNA <213> Homo sapiens <400> 240 cttaggacat gttgtaactg caggc 25 <210> 241 <211> 25 <212> DNA <213> Homo sapiens <400> 241 ttctgtgtca ttaaccatct ttaat 25 <210> 242 <211> 25 <212> DNA <213> Homo sapiens <400> 242 gtcattaacc atctttaatc aatgt 25 <210> 243 <211> 25 <212> DNA <213> Homo sapiens <400> 243 attaaccatc tttaatcaat gtgac 25 <210> 244 <211> 25 <212> DNA <213> Homo sapiens <400> 244 taatcaatgt gacatgttgc tcttt 25 <210> 245 <211> 25 <212> DNA <213> Homo sapiens <400> 245 tcaatgtgac atgttgctct ttggc 25 <210> 246 <211> 25 <212> DNA <213> Homo sapiens <400> 246 tgacatgttg ctctttggct gaaat 25 <210> 247 <211> 25 <212> DNA <213> Homo sapiens <400> 247 ctttggctga aattcttcaa cttgg 25 <210> 248 <211> 25 <212> DNA <213> Homo sapiens <400> 248 tggctgaaat tcttcaactt ggaaa 25 <210> 249 <211> 25 <212> DNA <213> Homo sapiens <400> 249 atgacacaga cccacagaag gtgtt 25 <210> 250 <211> 25 <212> DNA <213> Homo sapiens <400> 250 acacagaccc acagaaggtg ttcaa 25 <210> 251 <211> 25 <212> DNA <213> Homo sapiens <400> 251 tgttcaaaca caacctactc tgcaa 25 <210> 252 <211> 25 <212> DNA <213> Homo sapiens <400> 252 tactctgcaa accttggtaa aggaa 25 <210> 253 <211> 25 <212> DNA <213> Homo sapiens <400> 253 accagtcagc tggccagatt tcctc 25 <210> 254 <211> 25 <212> DNA <213> Homo sapiens <400> 254 agtcagctgg ccagatttcc tcact 25 <210> 255 <211> 25 <212> DNA <213> Homo sapiens <400> 255 agatttcctc actacctgcc atgca 25 <210> 256 <211> 25 <212> DNA <213> Homo sapiens <400> 256 tacctgccat gcatacatgc tgcgc 25 <210> 257 <211> 25 <212> DNA <213> Homo sapiens <400> 257 ccatgcatac atgctgcgca tgttt 25 <210> 258 <211> 25 <212> DNA <213> Homo sapiens <400> 258 tgcatacatg ctgcgcatgt tttct 25 <210> 259 <211> 25 <212> DNA <213> Homo sapiens <400> 259 gctgcgcatg ttttcttcat tcgta 25 <210> 260 <211> 25 <212> DNA <213> Homo sapiens <400> 260 gcgcatgttt tcttcattcg tatgt 25 <210> 261 <211> 25 <212> DNA <213> Homo sapiens <400> 261 attcatgcta ttgcaaacaa taaag 25 <210> 262 <211> 25 <212> DNA <213> Homo sapiens <400> 262 tttgaatctg gatcaacctt gaaaa 25 <210> 263 <211> 25 <212> DNA <213> Homo sapiens <400> 263 aaattcctgg aggaatctgt gtcaa 25 <210> 264 <211> 25 <212> DNA <213> Homo sapiens <400> 264 gtgtcaatga gccctgaaga acgag 25 <210> 265 <211> 25 <212> DNA <213> Homo sapiens <400> 265 gaacgagcca gatacctgga gaact 25 <210> 266 <211> 25 <212> DNA <213> Homo sapiens <400> 266 gccagatacc tggagaacta tgatg 25 <210> 267 <211> 25 <212> DNA <213> Homo sapiens <400> 267 gccatccgag ttactcatga gacca 25 <210> 268 <211> 25 <212> DNA <213> Homo sapiens <400> 268 cgagttactc atgagaccag tgccc 25 <210> 269 <211> 25 <212> DNA <213> Homo sapiens <400> 269 actcatgaga ccagtgccca tgaag 25 <210> 270 <211> 25 <212> DNA <213> Homo sapiens <400> 270 agtgcccatg aaggtcagac tgagg 25 <210> 271 <211> 25 <212> DNA <213> Homo sapiens <400> 271 ggtcagactg aggcaccaag tatag 25 <210> 272 <211> 25 <212> DNA <213> Homo sapiens <400> 272 actgaggcac caagtataga tgaga 25 <210> 273 <211> 25 <212> DNA <213> Homo sapiens <400> 273 aaagtagatc ttcattttat tgcat 25 <210> 274 <211> 25 <212> DNA <213> Homo sapiens <400> 274 ttagatgggc ggaagccatt tccaa 25 <210> 275 <211> 25 <212> DNA <213> Homo sapiens <400> 275 ttattagagg atgccataga agttt 25 <210> 276 <211> 25 <212> DNA <213> Homo sapiens <400> 276 aagtttatgg agcgcgaccc tgatg 25 <210> 277 <211> 25 <212> DNA <213> Homo sapiens <400> 277 cgcgaccctg atgaactaag attta 25 <210> 278 <211> 25 <212> DNA <213> Homo sapiens <400> 278 agatttaatg cgattgctct ttctg 25 <210> 279 <211> 25 <212> DNA <213> Homo sapiens <400> 279 ctttctgcag catagcttgt caata 25 <210> 280 <211> 25 <212> DNA <213> Homo sapiens <400> 280 attttctctg cccatacact aactc 25 <210> 281 <211> 25 <212> DNA <213> Homo sapiens <400> 281 actcaggagt tcatgctgag atgat 25 <210> 282 <211> 25 <212> DNA <213> Homo sapiens <400> 282 gagttcatgc tgagatgatc atgag 25 <210> 283 <211> 25 <212> DNA <213> Homo sapiens <400> 283 tcatgagttc atgcgacgta tattt 25 <210> 284 <211> 25 <212> DNA <213> Homo sapiens <400> 284 gttcatgcga cgtatatttt ccttt 25 <210> 285 <211> 25 <212> DNA <213> Homo sapiens <400> 285 tatattttcc tttggaaaca gaatg 25 <210> 286 <211> 25 <212> DNA <213> Homo sapiens <400> 286 cgttcttgat tagtatcgtg agttt 25 <210> 287 <211> 25 <212> DNA <213> Homo sapiens <400> 287 tgattagtat cgtgagtttg aaaag 25 <210> 288 <211> 25 <212> DNA <213> Homo sapiens <400> 288 tgtgagcatc gggctttgca gtccc 25 <210> 289 <211> 25 <212> DNA <213> Homo sapiens <400> 289 catcgggctt tgcagtccca tagaa 25 <210> 290 <211> 25 <212> DNA <213> Homo sapiens <400> 290 gctttgcagt cccatagaac agaaa 25 <210> 291 <211> 25 <212> DNA <213> Homo sapiens <400> 291 atgggatgct agcgtgccac tacct 25 <210> 292 <211> 25 <212> DNA <213> Homo sapiens <400> 292 tgctagcgtg ccactaccta cttgt 25 <210> 293 <211> 25 <212> DNA <213> Homo sapiens <400> 293 actacctact tgtgtgattg tggga 25 <210> 294 <211> 25 <212> DNA <213> Homo sapiens <400> 294 tacttgtgtg attgtgggaa attac 25 <210> 295 <211> 25 <212> DNA <213> Homo sapiens <400> 295 caatttcctc aaccataaaa tgaag 25 <210> 296 <211> 25 <212> DNA <213> Homo sapiens <400> 296 aatgcctacc tcagagggat gctga 25 <210> 297 <211> 25 <212> DNA <213> Homo sapiens <400> 297 agagggatgc tgaccacaga ccttt 25 <210> 298 <211> 25 <212> DNA <213> Homo sapiens <400> 298 accacagacc tttatagcag cccgt 25 <210> 299 <211> 25 <212> DNA <213> Homo sapiens <400> 299 gacctttata gcagcccgta tgata 25 <210> 300 <211> 25 <212> DNA <213> Homo sapiens <400> 300 agcccgtatg atattattca catta 25 <210> 301 <211> 25 <212> DNA <213> Homo sapiens <400> 301 caagcggtgc cagccacgag acgtc 25 <210> 302 <211> 25 <212> DNA <213> Homo sapiens <400> 302 gtgccagcca cgagacgtca ccaag 25 <210> 303 <211> 25 <212> DNA <213> Homo sapiens <400> 303 agacgtcacc aagaaaacat gcaac 25 <210> 304 <211> 25 <212> DNA <213> Homo sapiens <400> 304 gattcatgcg atgtgtccaa tcgga 25 <210> 305 <211> 25 <212> DNA <213> Homo sapiens <400> 305 tgcgatgtgt ccaatcggag acaaa 25 <210> 306 <211> 25 <212> DNA <213> Homo sapiens <400> 306 agacaaaagc agtttctctc caact 25 <210> 307 <211> 25 <212> DNA <213> Homo sapiens <400> 307 aagcagtttc tctccaactc cctct 25 <210> 308 <211> 25 <212> DNA <213> Homo sapiens <400> 308 tccaactccc tctgggaagg tgacc 25 <210> 309 <211> 25 <212> DNA <213> Homo sapiens <400> 309 tgggaaggtg acctggccag agcca 25 <210> 310 <211> 25 <212> DNA <213> Homo sapiens <400> 310 ggtgacctgg ccagagccaa gaaac 25 <210> 311 <211> 25 <212> DNA <213> Homo sapiens <400> 311 ctggccagag ccaagaaaca ctttc 25 <210> 312 <211> 25 <212> DNA <213> Homo sapiens <400> 312 actcaacaac caagcgcctg gagga 25 <210> 313 <211> 25 <212> DNA <213> Homo sapiens <400> 313 ctgccaccac tgggcaaaca gaaga 25 <210> 314 <211> 25 <212> DNA <213> Homo sapiens <400> 314 tctgtctttg gagagtattt tagaa 25 <210> 315 <211> 25 <212> DNA <213> Homo sapiens <400> 315 aactccaatg aaagacactg tttct 25 <210> 316 <211> 25 <212> DNA <213> Homo sapiens <400> 316 agacactgtt tctcctgttg gctca 25 <210> 317 <211> 25 <212> DNA <213> Homo sapiens <400> 317 tgtttctcct gttggctcac agggc 25 <210> 318 <211> 25 <212> DNA <213> Homo sapiens <400> 318 tggctcacag ggctgaaagg ggctt 25 <210> 319 <211> 25 <212> DNA <213> Homo sapiens <400> 319 ggggcttttg tcctcctggg tcagg 25 <210> 320 <211> 25 <212> DNA <213> Homo sapiens <400> 320 tttgtcctcc tgggtcaggg agaac 25 <210> 321 <211> 25 <212> DNA <213> Homo sapiens <400> 321 ctgcctgcct tggtctccca ataca 25 <210> 322 <211> 25 <212> DNA <213> Homo sapiens <400> 322 cttggtctcc caatacaggc atgaa 25 <210> 323 <211> 25 <212> DNA <213> Homo sapiens <400> 323 tcccaataca ggcatgaacc actgc 25 <210> 324 <211> 25 <212> DNA <213> Homo sapiens <400> 324 ctacttagat atttcatgtg ctata 25 <210> 325 <211> 25 <212> DNA <213> Homo sapiens <400> 325 atatttcatg tgctatagac attag 25 <210> 326 <211> 25 <212> DNA <213> Homo sapiens <400> 326 ttccatgaca tttttcctct ctgca 25 <210> 327 <211> 25 <212> DNA <213> Homo sapiens <400> 327 acatttttcc tctctgcaaa tggct 25 <210> 328 <211> 25 <212> DNA <213> Homo sapiens <400> 328 cctctctgca aatggcttag ctact 25 <210> 329 <211> 25 <212> DNA <213> Homo sapiens <400> 329 gcaaatggct tagctacttg tgttt 25 <210> 330 <211> 25 <212> DNA <213> Homo sapiens <400> 330 ttcccttttg gggcaagaca gactc 25 <210> 331 <211> 25 <212> DNA <213> Homo sapiens <400> 331 atatatcagt gttgtctcat agaac 25 <210> 332 <211> 25 <212> DNA <213> Homo sapiens <400> 332 gttgtctcat agaactgcct ggatt 25 <210> 333 <211> 25 <212> DNA <213> Homo sapiens <400> 333 catagaactg cctggattcc attta 25 <210> 334 <211> 25 <212> DNA <213> Homo sapiens <400> 334 ttttctgatt ccatcctgtg tcccc 25 <210> 335 <211> 25 <212> DNA <213> Homo sapiens <400> 335 catcctgtgt ccccttcatc cttga 25 <210> 336 <211> 25 <212> DNA <213> Homo sapiens <400> 336 ccttcatcct tgactccttt ggtat 25 <210> 337 <211> 25 <212> DNA <213> Homo sapiens <400> 337 cttgactcct ttggtatttc actga 25 <210> 338 <211> 25 <212> DNA <213> Homo sapiens <400> 338 ctttggtatt tcactgaatt tcaaa 25 <210> 339 <211> 25 <212> DNA <213> Homo sapiens <400> 339 atttcactga atttcaaaca tttgt 25 <210> 340 <211> 25 <212> DNA <213> Homo sapiens <400> 340 aatttcaaac atttgtcaga gaaga 25 <210> 341 <211> 25 <212> DNA <213> Homo sapiens <400> 341 cctctccact gtttcatgag cccaa 25 <210> 342 <211> 25 <212> DNA <213> Homo sapiens <400> 342 cactgtttca tgagcccaaa caccc 25 <210> 343 <211> 25 <212> DNA <213> Homo sapiens <400> 343 accctgcagc ttggagaact ccgct 25 <210> 344 <211> 25 <212> DNA <213> Homo sapiens <400> 344 cagcttggag aactccgctg gatgg 25 <210> 345 <211> 25 <212> DNA <213> Homo sapiens <400> 345 gggagtctca tgcaagactt cactg 25 <210> 346 <211> 25 <212> DNA <213> Homo sapiens <400> 346 ctcatgcaag acttcactgc agcct 25 <210> 347 <211> 25 <212> DNA <213> Homo sapiens <400> 347 caagacttca ctgcagcctt tcaca 25 <210> 348 <211> 25 <212> DNA <213> Homo sapiens <400> 348 ttcactgcag cctttcacag gactc 25 <210> 349 <211> 25 <212> DNA <213> Homo sapiens <400> 349 aggactctgc agatagtgcc tctgc 25 <210> 350 <211> 25 <212> DNA <213> Homo sapiens <400> 350 ctgcagatag tgcctctgca aacta 25 <210> 351 <211> 25 <212> DNA <213> Homo sapiens <400> 351 atagtgcctc tgcaaactaa ggagt 25 <210> 352 <211> 25 <212> DNA <213> Homo sapiens <400> 352 tcaggattgt ttctcggcac cagtg 25 <210> 353 <211> 25 <212> DNA <213> Homo sapiens <400> 353 ttgtttctcg gcaccagtgc ctcag 25 <210> 354 <211> 25 <212> DNA <213> Homo sapiens <400> 354 ctcggcacca gtgcctcagt gctgc 25 <210> 355 <211> 25 <212> DNA <213> Homo sapiens <400> 355 gcctcagtgc tgcaattgag ggcta 25 <210> 356 <211> 25 <212> DNA <213> Homo sapiens <400> 356 gtgctgcaat tgagggctaa atccc 25 <210> 357 <211> 25 <212> DNA <213> Homo sapiens <400> 357 caagtgtctc ttgactggct caaga 25 <210> 358 <211> 25 <212> DNA <213> Homo sapiens <400> 358 gactggctca agaattaggg cccca 25 <210> 359 <211> 25 <212> DNA <213> Homo sapiens <400> 359 gaagcatgta ctgtacttcc caatt 25 <210> 360 <211> 25 <212> DNA <213> Homo sapiens <400> 360 tgtactgtac ttcccaattg ccaca 25 <210> 361 <211> 25 <212> DNA <213> Homo sapiens <400> 361 cccatcattg tggtgaagca gggac 25 <210> 362 <211> 25 <212> DNA <213> Homo sapiens <400> 362 atcattgtgg tgaagcaggg acacg 25 <210> 363 <211> 25 <212> DNA <213> Homo sapiens <400> 363 tggagccaga tcactgctga ggtca 25 <210> 364 <211> 25 <212> DNA <213> Homo sapiens <400> 364 atcactgctg aggtcacaag cccca 25 <210> 365 <211> 25 <212> DNA <213> Homo sapiens <400> 365 gacgtgttca atgctaacag caacc 25 <210> 366 <211> 25 <212> DNA <213> Homo sapiens <400> 366 aatgctaaca gcaacctcag ttctg 25 <210> 367 <211> 25 <212> DNA <213> Homo sapiens <400> 367 gctaacagca acctcagttc tgggc 25 <210> 368 <211> 25 <212> DNA <213> Homo sapiens <400> 368 ctcagttctg ggcctctgcc catct 25 <210> 369 <211> 25 <212> DNA <213> Homo sapiens <400> 369 cagctagtga acaagcctgt agagg 25 <210> 370 <211> 25 <212> DNA <213> Homo sapiens <400> 370 gtgaacaagc ctgtagagga gctcc 25 <210> 371 <211> 25 <212> DNA <213> Homo sapiens <400> 371 gaacacctgt ccattgaaga tttca 25 <210> 372 <211> 25 <212> DNA <213> Homo sapiens <400> 372 tccattgaag atttcactca ggcct 25 <210> 373 <211> 25 <212> DNA <213> Homo sapiens <400> 373 ttcactcagg cctttgggat gactc 25 <210> 374 <211> 25 <212> DNA <213> Homo sapiens <400> 374 gcctttggga tgactccagc tgcct 25 <210> 375 <211> 25 <212> DNA <213> Homo sapiens <400> 375 gctctgcctc gatggaagca acaaa 25 <210> 376 <211> 25 <212> DNA <213> Homo sapiens <400> 376 tagggtctca ttttctcacc gatat 25 <210> 377 <211> 25 <212> DNA <213> Homo sapiens <400> 377 aattttgcag atgtgcctat gagca 25 <210> 378 <211> 25 <212> DNA <213> Homo sapiens <400> 378 gcagatgtgc ctatgagcac aaact 25 <210> 379 <211> 25 <212> DNA <213> Homo sapiens <400> 379 gtgcctatga gcacaaactt ctgtg 25 <210> 380 <211> 25 <212> DNA <213> Homo sapiens <400> 380 ttctgtggca aatgccagtt ttgtt 25 <210> 381 <211> 25 <212> DNA <213> Homo sapiens <400> 381 ctgcactgag gcagcgacct caagt 25 <210> 382 <211> 25 <212> DNA <213> Homo sapiens <400> 382 agcgacctca agtgtcctga aagct 25 <210> 383 <211> 25 <212> DNA <213> Homo sapiens <400> 383 ctcaagtgtc ctgaaagctt acatt 25 <210> 384 <211> 25 <212> DNA <213> Homo sapiens <400> 384 gaaagcttac attttcctga gacct 25 <210> 385 <211> 25 <212> DNA <213> Homo sapiens <400> 385 tttcctgaga cctcaatgaa gcaga 25 <210> 386 <211> 25 <212> DNA <213> Homo sapiens <400> 386 gagacctcaa tgaagcagaa atgta 25 <210> 387 <211> 25 <212> DNA <213> Homo sapiens <400> 387 acttaggctt ggctgccctg tctgg 25 <210> 388 <211> 25 <212> DNA <213> Homo sapiens <400> 388 gcttggctgc cctgtctgga acatg 25 <210> 389 <211> 25 <212> DNA <213> Homo sapiens <400> 389 ctgccctgtc tggaacatgg acttt 25 <210> 390 <211> 25 <212> DNA <213> Homo sapiens <400> 390 tgtctggaac atggactttc ttgca 25 <210> 391 <211> 25 <212> DNA <213> Homo sapiens <400> 391 ggactttctt gcatgaatca gatgt 25 <210> 392 <211> 25 <212> DNA <213> Homo sapiens <400> 392 cagatgtgtg ttctcagtga aataa 25 <210> 393 <211> 25 <212> DNA <213> Homo sapiens <400> 393 ctcagtgaaa taactacctt ccact 25 <210> 394 <211> 25 <212> DNA <213> Homo sapiens <400> 394 gaaataacta ccttccactc tggaa 25 <210> 395 <211> 25 <212> DNA <213> Homo sapiens <400> 395 actaccttcc actctggaac cttat 25 <210> 396 <211> 25 <212> DNA <213> Homo sapiens <400> 396 accttattcc agcagttgtt ccagg 25 <210> 397 <211> 25 <212> DNA <213> Homo sapiens <400> 397 cagttgttcc agggagcttc tacct 25 <210> 398 <211> 25 <212> DNA <213> Homo sapiens <400> 398 ctatctagaa cttgagaaga tttta 25 <210> 399 <211> 25 <212> DNA <213> Homo sapiens <400> 399 tactggcttt ccttctgtat tcata 25 <210> 400 <211> 25 <212> DNA <213> Homo sapiens <400> 400 taaataccca tcttcattaa agtat 25 <210> 401 <211> 25 <212> DNA <213> Homo sapiens <400> 401 ggaacacatc aaagagctgg cggcc 25 <210> 402 <211> 25 <212> DNA <213> Homo sapiens <400> 402 catcaaagag ctggcggccg acgtg 25 <210> 403 <211> 25 <212> DNA <213> Homo sapiens <400> 403 gtgaagccag ctgcaggaat caaaa 25 <210> 404 <211> 25 <212> DNA <213> Homo sapiens <400> 404 taaatatcat cctgccttca tggaa 25 <210> 405 <211> 25 <212> DNA <213> Homo sapiens <400> 405 tcatcctgcc ttcatggaac tcagc 25 <210> 406 <211> 25 <212> DNA <213> Homo sapiens <400> 406 catggaactc agctgtctgt ggctt 25 <210> 407 <211> 25 <212> DNA <213> Homo sapiens <400> 407 gtggcttccc atgtctttct ccaaa 25 <210> 408 <211> 25 <212> DNA <213> Homo sapiens <400> 408 gtctttctcc aaagccatcc agagg 25 <210> 409 <211> 25 <212> DNA <213> Homo sapiens <400> 409 agccatccag agggttgtga ttttg 25 <210> 410 <211> 25 <212> DNA <213> Homo sapiens <400> 410 gattttgtct gcttagtatc tcatc 25 <210> 411 <211> 25 <212> DNA <213> Homo sapiens <400> 411 gtctgcttag tatctcatca acaaa 25 <210> 412 <211> 25 <212> DNA <213> Homo sapiens <400> 412 ttagtatctc atcaacaaag aaata 25 <210> 413 <211> 25 <212> DNA <213> Homo sapiens <400> 413 tcttcatggc catagctttt attca 25 <210> 414 <211> 25 <212> DNA <213> Homo sapiens <400> 414 tggccatagc ttttattcat tagct 25 <210> 415 <211> 25 <212> DNA <213> Homo sapiens <400> 415 ttttcatgtt cttgcagtca tcaga 25 <210> 416 <211> 25 <212> DNA <213> Homo sapiens <400> 416 atattttcca gggcaatcaa tgttc 25 <210> 417 <211> 25 <212> DNA <213> Homo sapiens <400> 417 gcaatcaatg ttcacgcaac ttgaa 25 <210> 418 <211> 25 <212> DNA <213> Homo sapiens <400> 418 ggtcttcaaa ttgtcttttg tcatg 25 <210> 419 <211> 25 <212> DNA <213> Homo sapiens <400> 419 caaattgtct tttgtcatgt ggcta 25 <210> 420 <211> 25 <212> DNA <213> Homo sapiens <400> 420 gtcttttgtc atgtggctaa atgcc 25 <210> 421 <211> 25 <212> DNA <213> Homo sapiens <400> 421 attaaattct agtgaagttg atata 25 <210> 422 <211> 25 <212> DNA <213> Homo sapiens <400> 422 agtagcaaat tcactaaact tttgt 25 <210> 423 <211> 25 <212> DNA <213> Homo sapiens <400> 423 cactaaactt ttgtgttcag agtta 25 <210> 424 <211> 25 <212> DNA <213> Homo sapiens <400> 424 tctcagtact ttcaatgtag gggaa 25 <210> 425 <211> 25 <212> DNA <213> Homo sapiens <400> 425 acatagtgtg tatgtttggg tttta 25 <210> 426 <211> 25 <212> DNA <213> Homo sapiens <400> 426 agtgttaatt ctatactgca tttga 25 <210> 427 <211> 25 <212> DNA <213> Homo sapiens <400> 427 atactgcatt tgaagtgtat gtaac 25 <210> 428 <211> 25 <212> DNA <213> Homo sapiens <400> 428 ttatgttaat gcagtcatga agttg 25 <210> 429 <211> 25 <212> DNA <213> Homo sapiens <400> 429 atgaagttgg tttgctccag catcc 25 <210> 430 <211> 25 <212> DNA <213> Homo sapiens <400> 430 ctccagcatc cggtagtctt taaac 25 <210> 431 <211> 25 <212> DNA <213> Homo sapiens <400> 431 tttaaacatt cttttagtga aattg 25 <210> 432 <211> 25 <212> DNA <213> Homo sapiens <400> 432 gttttatcag tgctaatgtg tgcaa 25 <210> 433 <211> 25 <212> DNA <213> Homo sapiens <400> 433 tattttgctt ttctcctggc atcag 25 <210> 434 <211> 25 <212> DNA <213> Homo sapiens <400> 434 tcctggcatc agaaagtggt ggcgt 25 <210> 435 <211> 25 <212> DNA <213> Homo sapiens <400> 435 ggtggcgttt tctgtactgg attgc 25 <210> 436 <211> 25 <212> DNA <213> Homo sapiens <400> 436 tctgtactgg attgcaccaa ggaag 25 <210> 437 <211> 25 <212> DNA <213> Homo sapiens <400> 437 ttgcaccaag gaagcttttg gggag 25 <210> 438 <211> 25 <212> DNA <213> Homo sapiens <400> 438 atgaaaagag ccctttatca ataca 25 <210> 439 <211> 25 <212> DNA <213> Homo sapiens <400> 439 atcaatacag tgctgcaatt tctgg 25 <210> 440 <211> 25 <212> DNA <213> Homo sapiens <400> 440 ctgcaatttc tggatatcag ctaca 25 <210> 441 <211> 25 <212> DNA <213> Homo sapiens <400> 441 tccatgatta agctgttaga ataac 25 <210> 442 <211> 25 <212> DNA <213> Homo sapiens <400> 442 gaataacttg ctactcatgg caaga 25 <210> 443 <211> 25 <212> DNA <213> Homo sapiens <400> 443 actcatggca agattaggaa gtcac 25 <210> 444 <211> 25 <212> DNA <213> Homo sapiens <400> 444 aagtcacaga ttcttttcta taatg 25 <210> 445 <211> 25 <212> DNA <213> Homo sapiens <400> 445 cttttctata atgtgcctgg ctctg 25 <210> 446 <211> 25 <212> DNA <213> Homo sapiens <400> 446 tataatgtgc ctggctctga ttctt 25 <210> 447 <211> 25 <212> DNA <213> Homo sapiens <400> 447 gtgcctggct ctgattcttc attat 25 <210> 448 <211> 25 <212> DNA <213> Homo sapiens <400> 448 ggctctgatt cttcattatg tatgt 25 <210> 449 <211> 25 <212> DNA <213> Homo sapiens <400> 449 tttcatggaa aagccttctt atatc 25 <210> 450 <211> 25 <212> DNA <213> Homo sapiens <400> 450 ggaaaagcct tcttatatct gaaga 25 <210> 451 <211> 25 <212> DNA <213> Homo sapiens <400> 451 gccttcttat atctgaagat tgaaa 25 <210> 452 <211> 25 <212> DNA <213> Homo sapiens <400> 452 atccagagaa agcaccttat gagtg 25 <210> 453 <211> 25 <212> DNA <213> Homo sapiens <400> 453 caccttatga gtggaagaat ttgga 25 <210> 454 <211> 25 <212> DNA <213> Homo sapiens <400> 454 atttggatgg catttcccat gcacc 25 <210> 455 <211> 25 <212> DNA <213> Homo sapiens <400> 455 atggcatttc ccatgcacct aatgc 25 <210> 456 <211> 25 <212> DNA <213> Homo sapiens <400> 456 tttcccatgc acctaatgct gtgaa 25 <210> 457 <211> 25 <212> DNA <213> Homo sapiens <400> 457 ctaatgctgt gaaccccgca tttta 25 <210> 458 <211> 25 <212> DNA <213> Homo sapiens <400> 458 tgatttatca gttcagtcca attta 25 <210> 459 <211> 25 <212> DNA <213> Homo sapiens <400> 459 atcagttcag tccaatttat actgg 25 <210> 460 <211> 25 <212> DNA <213> Homo sapiens <400> 460 gaaatatttc ttcatttcag caatg 25 <210> 461 <211> 25 <212> DNA <213> Homo sapiens <400> 461 gccgttggca accacaggtc attca 25 <210> 462 <211> 25 <212> DNA <213> Homo sapiens <400> 462 tcattcagcg acaagaatgg cctca 25 <210> 463 <211> 25 <212> DNA <213> Homo sapiens <400> 463 gagctgcggc ccgaagatga catga 25 <210> 464 <211> 25 <212> DNA <213> Homo sapiens <400> 464 ggaagctttg acaggtccat acctg 25 <210> 465 <211> 25 <212> DNA <213> Homo sapiens <400> 465 tttgacaggt ccatacctga aaaca 25 <210> 466 <211> 25 <212> DNA <213> Homo sapiens <400> 466 atgcgcacaa tcattgagtt tctgt 25 <210> 467 <211> 25 <212> DNA <213> Homo sapiens <400> 467 tttctgtctt tcttgcatct caaag 25 <210> 468 <211> 25 <212> DNA <213> Homo sapiens <400> 468 tctttcttgc atctcaaaga ggccg 25 <210> 469 <211> 25 <212> DNA <213> Homo sapiens <400> 469 ttgcatctca aagaggccgg tgccc 25 <210> 470 <211> 25 <212> DNA <213> Homo sapiens <400> 470 tcagaagaca tcgagcggtc ctgag 25 <210> 471 <211> 25 <212> DNA <213> Homo sapiens <400> 471 gagcggtcct gagagcctcc tgggc 25 <210> 472 <211> 25 <212> DNA <213> Homo sapiens <400> 472 tgtctgtgtg ctgtaacctg aagtc 25 <210> 473 <211> 25 <212> DNA <213> Homo sapiens <400> 473 tgtgctgtaa cctgaagtca aacct 25 <210> 474 <211> 25 <212> DNA <213> Homo sapiens <400> 474 gtaacctgaa gtcaaacctt aagat 25 <210> 475 <211> 25 <212> DNA <213> Homo sapiens <400> 475 tgaagtcaaa ccttaagata atgga 25 <210> 476 <211> 25 <212> DNA <213> Homo sapiens <400> 476 taatggataa tcttcggcca attta 25 <210> 477 <211> 25 <212> DNA <213> Homo sapiens <400> 477 ataatcttcg gccaatttat gcgga 25 <210> 478 <211> 25 <212> DNA <213> Homo sapiens <400> 478 ttcggccaat ttatgcggag tcagc 25 <210> 479 <211> 25 <212> DNA <213> Homo sapiens <400> 479 atgcggagtc agccattcct gttct 25 <210> 480 <211> 25 <212> DNA <213> Homo sapiens <400> 480 agtcagccat tcctgttctc tttgc 25 <210> 481 <211> 25 <212> DNA <213> Homo sapiens <400> 481 tcacattgga gtcactgcca agtca 25 <210> 482 <211> 25 <212> DNA <213> Homo sapiens <400> 482 ggagtcactg ccaagtcata gccat 25 <210> 483 <211> 25 <212> DNA <213> Homo sapiens <400> 483 aagtcatagc cataaatgat gagtc 25 <210> 484 <211> 25 <212> DNA <213> Homo sapiens <400> 484 atgatgagtc ggtcctcttt ccagt 25 <210> 485 <211> 25 <212> DNA <213> Homo sapiens <400> 485 ggtcctcttt ccagtggatc ataag 25 <210> 486 <211> 25 <212> DNA <213> Homo sapiens <400> 486 tcataagaca atggaccctt tttgt 25 <210> 487 <211> 25 <212> DNA <213> Homo sapiens <400> 487 agaatttcaa aatttctgca ttcac 25 <210> 488 <211> 25 <212> DNA <213> Homo sapiens <400> 488 tatatagagc acctggaagc agtaa 25 <210> 489 <211> 25 <212> DNA <213> Homo sapiens <400> 489 cacctggaag cagtaacatg caaat 25 <210> 490 <211> 25 <212> DNA <213> Homo sapiens <400> 490 aagtccatga aaactcacag catga 25 <210> 491 <211> 25 <212> DNA <213> Homo sapiens <400> 491 ctcacagcat gattgacagt agttt 25 <210> 492 <211> 25 <212> DNA <213> Homo sapiens <400> 492 attagcagcc atagctgcct ttatg 25 <210> 493 <211> 25 <212> DNA <213> Homo sapiens <400> 493 gcctttatgt ctgctgtgat cctca 25 <210> 494 <211> 25 <212> DNA <213> Homo sapiens <400> 494 gtctgctgtg atcctcacag ctgtt 25 <210> 495 <211> 25 <212> DNA <213> Homo sapiens <400> 495 cacagctgtt gctgttatta cagtc 25 <210> 496 <211> 25 <212> DNA <213> Homo sapiens <400> 496 ctgttattac agtccagctt agaag 25 <210> 497 <211> 25 <212> DNA <213> Homo sapiens <400> 497 cagtccagct tagaagacaa tacgt 25 <210> 498 <211> 25 <212> DNA <213> Homo sapiens <400> 498 gaaacttcga caagagaatg gaaat 25 <210> 499 <211> 25 <212> DNA <213> Homo sapiens <400> 499 gtacatgcta tagcataact gaaga 25 <210> 500 <211> 25 <212> DNA <213> Homo sapiens <400> 500 tacaggatat cacattggag tcact 25 <210> 501 <211> 25 <212> DNA <213> Homo sapiens <400> 501 cactgcactg tctgagaatt tccaa 25 <210> 502 <211> 25 <212> DNA <213> Homo sapiens <400> 502 tgcactgtct gagaatttcc aaaac 25 <210> 503 <211> 25 <212> DNA <213> Homo sapiens <400> 503 aaactttaat gaactaactg acagc 25 <210> 504 <211> 25 <212> DNA <213> Homo sapiens <400> 504 ctttaatgaa ctaactgaca gcttc 25 <210> 505 <211> 25 <212> DNA <213> Homo sapiens <400> 505 aactgacagc ttcatgaaac tgtcc 25 <210> 506 <211> 25 <212> DNA <213> Homo sapiens <400> 506 cagcttcatg aaactgtcca ccaag 25 <210> 507 <211> 25 <212> DNA <213> Homo sapiens <400> 507 cttcatgaaa ctgtccacca agatc 25 <210> 508 <211> 25 <212> DNA <213> Homo sapiens <400> 508 actgtccacc aagatcaagc agaga 25 <210> 509 <211> 25 <212> DNA <213> Homo sapiens <400> 509 gtccaccaag atcaagcaga gaaaa 25 <210> 510 <211> 25 <212> DNA <213> Homo sapiens <400> 510 attaatttca tgggactaaa tgaac 25 <210> 511 <211> 25 <212> DNA <213> Homo sapiens <400> 511 ttaaatgtct tgtttcccag atttc 25 <210> 512 <211> 25 <212> DNA <213> Homo sapiens <400> 512 aatgtcttgt ttcccagatt tcagg 25 <210> 513 <211> 25 <212> DNA <213> Homo sapiens <400> 513 agctatccac tcttacagca atttg 25 <210> 514 <211> 25 <212> DNA <213> Homo sapiens <400> 514 ccactcttac agcaatttga taaaa 25 <210> 515 <211> 25 <212> DNA <213> Homo sapiens <400> 515 ttacattttc tccctatgtg gtcgc 25 <210> 516 <211> 25 <212> DNA <213> Homo sapiens <400> 516 cattttctcc ctatgtggtc gctcc 25 <210> 517 <211> 25 <212> DNA <213> Homo sapiens <400> 517 cctatgtggt cgctccagac ttggg 25 <210> 518 <211> 25 <212> DNA <213> Homo sapiens <400> 518 atgtggtcgc tccagacttg ggaaa 25 <210> 519 <211> 25 <212> DNA <213> Homo sapiens <400> 519 tggtcgctcc agacttggga aacta 25 <210> 520 <211> 25 <212> DNA <213> Homo sapiens <400> 520 aatctgctct ttgtataaca gaaaa 25 <210> 521 <211> 25 <212> DNA <213> Homo sapiens <400> 521 ccaacaacac caggtatggc ctggc 25 <210> 522 <211> 25 <212> DNA <213> Homo sapiens <400> 522 gcctggctgc ggctgtgttc acccg 25 <210> 523 <211> 25 <212> DNA <213> Homo sapiens <400> 523 ctgtgttcac ccgggatctg gacaa 25 <210> 524 <211> 25 <212> DNA <213> Homo sapiens <400> 524 tcacccggga tctggacaag gccat 25 <210> 525 <211> 25 <212> DNA <213> Homo sapiens <400> 525 gggatctgga caaggccatg tactt 25 <210> 526 <211> 25 <212> DNA <213> Homo sapiens <400> 526 tggacaaggc catgtacttc accca 25 <210> 527 <211> 25 <212> DNA <213> Homo sapiens <400> 527 tgtacttcac ccaggcactc caggc 25 <210> 528 <211> 25 <212> DNA <213> Homo sapiens <400> 528 gggtaaacac ctacaacatc gtcac 25 <210> 529 <211> 25 <212> DNA <213> Homo sapiens <400> 529 cctgccacac gccatttgga gggtt 25 <210> 530 <211> 25 <212> DNA <213> Homo sapiens <400> 530 atgggcttaa ggcctacaca gaggt 25 <210> 531 <211> 25 <212> DNA <213> Homo sapiens <400> 531 ttaaggccta cacagaggta aagac 25 <210> 532 <211> 25 <212> DNA <213> Homo sapiens <400> 532 cggtcaccat caaggttcct cagaa 25 <210> 533 <211> 25 <212> DNA <213> Homo sapiens <400> 533 ctcagaagaa ctcgtaaggc agctg 25 <210> 534 <211> 25 <212> DNA <213> Homo sapiens <400> 534 cgtaaggcag ctgtcaggga ggccc 25 <210> 535 <211> 25 <212> DNA <213> Homo sapiens <400> 535 gcagctgtca gggaggccca gtcac 25 <210> 536 <211> 25 <212> DNA <213> Homo sapiens <400> 536 gtcagggagg cccagtcaca gtcca 25 <210> 537 <211> 25 <212> DNA <213> Homo sapiens <400> 537 gaggcccagt cacagtccag caatt 25 <210> 538 <211> 25 <212> DNA <213> Homo sapiens <400> 538 accaccttga cgaatgcttg ccaag 25 <210> 539 <211> 25 <212> DNA <213> Homo sapiens <400> 539 aatgcttgcc aagctgtttt aaagc 25 <210> 540 <211> 25 <212> DNA <213> Homo sapiens <400> 540 tgccaagctg ttttaaagcc aagaa 25 <210> 541 <211> 25 <212> DNA <213> Homo sapiens <400> 541 ttgtctgtgt gcgcaaatcc aaaac 25 <210> 542 <211> 25 <212> DNA <213> Homo sapiens <400> 542 gtgtgcgcaa atccaaaaca gactt 25 <210> 543 <211> 25 <212> DNA <213> Homo sapiens <400> 543 gcaaatccaa aacagacttg ggtga 25 <210> 544 <211> 25 <212> DNA <213> Homo sapiens <400> 544 attgtgcgtc tcctcagtaa aaaag 25 <210> 545 <211> 25 <212> DNA <213> Homo sapiens <400> 545 cgtctcctca gtaaaaaagt caaga 25 <210> 546 <211> 25 <212> DNA <213> Homo sapiens <400> 546 cttttctgga atggaattgg acata 25 <210> 547 <211> 25 <212> DNA <213> Homo sapiens <400> 547 ggaattggac atagcccaag aacag 25 <210> 548 <211> 25 <212> DNA <213> Homo sapiens <400> 548 cttgcacatc atggagggtt tagtg 25 <210> 549 <211> 25 <212> DNA <213> Homo sapiens <400> 549 gcttatctaa tttgtgcctc acctg 25 <210> 550 <211> 25 <212> DNA <213> Homo sapiens <400> 550 ctaatttgtg cctcacctgg acttg 25 <210> 551 <211> 25 <212> DNA <213> Homo sapiens <400> 551 tgtgcctcac ctggacttgt ccaat 25 <210> 552 <211> 25 <212> DNA <213> Homo sapiens <400> 552 tcacctggac ttgtccaatt aatga 25 <210> 553 <211> 25 <212> DNA <213> Homo sapiens <400> 553 attcatattg catcatagtt tgctt 25 <210> 554 <211> 25 <212> DNA <213> Homo sapiens <400> 554 tcatagtttg ctttgtttaa gcatc 25 <210> 555 <211> 25 <212> DNA <213> Homo sapiens <400> 555 ttgtttaagc atcacattaa agtga 25 <210> 556 <211> 25 <212> DNA <213> Homo sapiens <400> 556 aagcatcaca ttaaagtgaa actgt 25 <210> 557 <211> 25 <212> DNA <213> Homo sapiens <400> 557 tattttggtt tagtgcaaag tataa 25 <210> 558 <211> 25 <212> DNA <213> Homo sapiens <400> 558 attatatgga ctttcttgca agcaa 25 <210> 559 <211> 25 <212> DNA <213> Homo sapiens <400> 559 tggactttct tgcaagcaac aagct 25 <210> 560 <211> 25 <212> DNA <213> Homo sapiens <400> 560 ttcttgcaag caacaagcta ttttt 25 <210> 561 <211> 25 <212> DNA <213> Homo sapiens <400> 561 tagtcaaact ctcatttagg tgaca 25 <210> 562 <211> 25 <212> DNA <213> Homo sapiens <400> 562 aactctcatt taggtgacag cctgg 25 <210> 563 <211> 25 <212> DNA <213> Homo sapiens <400> 563 catttaggtg acagcctggt gactc 25 <210> 564 <211> 25 <212> DNA <213> Homo sapiens <400> 564 ggtgacagcc tggtgactcc agaat 25 <210> 565 <211> 25 <212> DNA <213> Homo sapiens <400> 565 gtgactccag aatggagcca gtcat 25 <210> 566 <211> 25 <212> DNA <213> Homo sapiens <400> 566 ccagaatgga gccagtcatg ctaaa 25 <210> 567 <211> 25 <212> DNA <213> Homo sapiens <400> 567 tggagccagt catgctaaat gccat 25 <210> 568 <211> 25 <212> DNA <213> Homo sapiens <400> 568 atgccatata ctcacactga aacat 25 <210> 569 <211> 25 <212> DNA <213> Homo sapiens <400> 569 cacactgaaa catgaggaag caggt 25 <210> 570 <211> 25 <212> DNA <213> Homo sapiens <400> 570 tagatcccag aacagacaaa acttt 25 <210> 571 <211> 25 <212> DNA <213> Homo sapiens <400> 571 gagagtccag gctgtctgag tcagc 25 <210> 572 <211> 25 <212> DNA <213> Homo sapiens <400> 572 ccaggctgtc tgagtcagca cagta 25 <210> 573 <211> 25 <212> DNA <213> Homo sapiens <400> 573 gtcctttctg ctttaactct tagaa 25 <210> 574 <211> 25 <212> DNA <213> Homo sapiens <400> 574 ttctgaaatt aaccaatcag tttat 25 <210> 575 <211> 25 <212> DNA <213> Homo sapiens <400> 575 tattcttctg ttcctggatt cccat 25 <210> 576 <211> 25 <212> DNA <213> Homo sapiens <400> 576 tctgttcctg gattcccatt ttaca 25 <210> 577 <211> 25 <212> DNA <213> Homo sapiens <400> 577 attttgcaga atggaaactg ccctg 25 <210> 578 <211> 25 <212> DNA <213> Homo sapiens <400> 578 cagaatggaa actgccctga ctctt 25 <210> 579 <211> 25 <212> DNA <213> Homo sapiens <400> 579 ggaaactgcc ctgactcttg aatca 25 <210> 580 <211> 25 <212> DNA <213> Homo sapiens <400> 580 tgccctgact cttgaatcac aaata 25 <210> 581 <211> 25 <212> DNA <213> Homo sapiens <400> 581 ttgtacctct aagtacatat gtaga 25 <210> 582 <211> 25 <212> DNA <213> Homo sapiens <400> 582 atattgtttt ctgtaaataa cctat 25 <210> 583 <211> 25 <212> DNA <213> Homo sapiens <400> 583 attctctcca atttgtttaa agaat 25 <210> 584 <211> 25 <212> DNA <213> Homo sapiens <400> 584 taaagtccaa agtctgatct ggtct 25 <210> 585 <211> 25 <212> DNA <213> Homo sapiens <400> 585 ccaaagtctg atctggtcta gttaa 25 <210> 586 <211> 25 <212> DNA <213> Homo sapiens <400> 586 ctggtctagt taacctagaa gtatt 25 <210> 587 <211> 25 <212> DNA <213> Homo sapiens <400> 587 ttttgtctct tagaaatact tgtga 25 <210> 588 <211> 25 <212> DNA <213> Homo sapiens <400> 588 ataatacaaa agggtcttga ctcta 25 <210> 589 <211> 25 <212> DNA <213> Homo sapiens <400> 589 aattgtcaca gacaagtaat gtcga 25 <210> 590 <211> 25 <212> DNA <213> Homo sapiens <400> 590 ttcaactaac ttttgcactg aggta 25 <210> 591 <211> 25 <212> DNA <213> Homo sapiens <400> 591 taacttttgc actgaggtac ctgaa 25 <210> 592 <211> 25 <212> DNA <213> Homo sapiens <400> 592 acttctttac aaaagccact ccact 25 <210> 593 <211> 25 <212> DNA <213> Homo sapiens <400> 593 caacggtgac actcagtatg tctgc 25 <210> 594 <211> 25 <212> DNA <213> Homo sapiens <400> 594 tcagtatgtc tgcagatgta cccct 25 <210> 595 <211> 25 <212> DNA <213> Homo sapiens <400> 595 gacacttaaa atactacttg gctcc 25 <210> 596 <211> 25 <212> DNA <213> Homo sapiens <400> 596 tggctcccaa gatcgaggat gaaga 25 <210> 597 <211> 25 <212> DNA <213> Homo sapiens <400> 597 tgagaactgc ttctaagatg ccagc 25 <210> 598 <211> 25 <212> DNA <213> Homo sapiens <400> 598 tgccagcata tactgaagtc ttttc 25 <210> 599 <211> 25 <212> DNA <213> Homo sapiens <400> 599 catatactga agtcttttct gtcac 25 <210> 600 <211> 25 <212> DNA <213> Homo sapiens <400> 600 tcttttctgt caccaaattt gtacc 25 <210> 601 <211> 25 <212> DNA <213> Homo sapiens <400> 601 gcgtgtgtgt tggtgtctga cattc 25 <210> 602 <211> 25 <212> DNA <213> Homo sapiens <400> 602 gtgttggtgt ctgacattct gggat 25 <210> 603 <211> 25 <212> DNA <213> Homo sapiens <400> 603 gtgtctgaca ttctgggatc tgcca 25 <210> 604 <211> 25 <212> DNA <213> Homo sapiens <400> 604 gacgttcagc ctaccatgaa tgcca 25 <210> 605 <211> 25 <212> DNA <213> Homo sapiens <400> 605 ctgcatcctg gtggcaagga cggag 25 <210> 606 <211> 25 <212> DNA <213> Homo sapiens <400> 606 gagcttcaca ttgcagaaca tcctg 25 <210> 607 <211> 25 <212> DNA <213> Homo sapiens <400> 607 cacattgcag aacatcctgc aggac 25 <210> 608 <211> 25 <212> DNA <213> Homo sapiens <400> 608 catcctgcag gacatctagt tctga 25 <210> 609 <211> 25 <212> DNA <213> Homo sapiens <400> 609 cttgtagagc atgttggtat gaaga 25 <210> 610 <211> 25 <212> DNA <213> Homo sapiens <400> 610 atgaagaacc tgaagtcctg ggcta 25 <210> 611 <211> 25 <212> DNA <213> Homo sapiens <400> 611 aacctgaagt cctgggctag tgtaa 25 <210> 612 <211> 25 <212> DNA <213> Homo sapiens <400> 612 aagtcctggg ctagtgtaaa tcgag 25 <210> 613 <211> 25 <212> DNA <213> Homo sapiens <400> 613 agtgtaaatc gaggtgccat tattc 25 <210> 614 <211> 25 <212> DNA <213> Homo sapiens <400> 614 ggtgccatta ttctttctag cctcc 25 <210> 615 <211> 25 <212> DNA <213> Homo sapiens <400> 615 ctccagagtt gtgacctgga agttg 25 <210> 616 <211> 25 <212> DNA <213> Homo sapiens <400> 616 agttgtgacc tggaagttgc aaaca 25 <210> 617 <211> 25 <212> DNA <213> Homo sapiens <400> 617 aaagctgcac tgaaaagctt gattc 25 <210> 618 <211> 25 <212> DNA <213> Homo sapiens <400> 618 gttctgtttc ccaatgcaga aaaga 25 <210> 619 <211> 25 <212> DNA <213> Homo sapiens <400> 619 tagggtccac catactggta attgg 25 <210> 620 <211> 25 <212> DNA <213> Homo sapiens <400> 620 ccaccatact ggtaattggg gtact 25 <210> 621 <211> 25 <212> DNA <213> Homo sapiens <400> 621 gacctatacc tagtggaatg caggg 25 <210> 622 <211> 25 <212> DNA <213> Homo sapiens <400> 622 tacctagtgg aatgcagggt cccag 25 <210> 623 <211> 25 <212> DNA <213> Homo sapiens <400> 623 tgcagggtcc cagtccaatt aacat 25 <210> 624 <211> 25 <212> DNA <213> Homo sapiens <400> 624 gtcccagtcc aattaacatg ggggc 25 <210> 625 <211> 25 <212> DNA <213> Homo sapiens <400> 625 agggatccag acaggtccca gtcat 25 <210> 626 <211> 25 <212> DNA <213> Homo sapiens <400> 626 gtcccgggca gaaccaagtc actcc 25 <210> 627 <211> 25 <212> DNA <213> Homo sapiens <400> 627 accaagtcac tccacaggat catga 25 <210> 628 <211> 25 <212> DNA <213> Homo sapiens <400> 628 atcatgagaa ggctgctttg attat 25 <210> 629 <211> 25 <212> DNA <213> Homo sapiens <400> 629 agaaggctgc tttgattatg caggt 25 <210> 630 <211> 25 <212> DNA <213> Homo sapiens <400> 630 ctgctttgat tatgcaggtt ctaca 25 <210> 631 <211> 25 <212> DNA <213> Homo sapiens <400> 631 tgcaggttct acaactgact gcaga 25 <210> 632 <211> 25 <212> DNA <213> Homo sapiens <400> 632 ttgccatgtt gcctcctgag caaag 25 <210> 633 <211> 25 <212> DNA <213> Homo sapiens <400> 633 tgttgcctcc tgagcaaagg cagag 25 <210> 634 <211> 25 <212> DNA <213> Homo sapiens <400> 634 ctcctgagca aaggcagagt atcct 25 <210> 635 <211> 25 <212> DNA <213> Homo sapiens <400> 635 ctggagcacc ttgataggtt ttcaa 25 <210> 636 <211> 25 <212> DNA <213> Homo sapiens <400> 636 atcctaaccc ttgaatgact caaat 25 <210> 637 <211> 25 <212> DNA <213> Homo sapiens <400> 637 acccttgaat gactcaaatc agtgc 25 <210> 638 <211> 25 <212> DNA <213> Homo sapiens <400> 638 ggaggactcc catcaccttc tctca 25 <210> 639 <211> 25 <212> DNA <213> Homo sapiens <400> 639 agaacaaaat cacttcattt tattg 25 <210> 640 <211> 25 <212> DNA <213> Homo sapiens <400> 640 ctttgaacac aattttagta cactg 25 <210> 641 <211> 25 <212> DNA <213> Homo sapiens <400> 641 ggaattatca cccatcatca tccaa 25 <210> 642 <211> 25 <212> DNA <213> Homo sapiens <400> 642 atcacccatc atcatccaat cgcag 25 <210> 643 <211> 25 <212> DNA <213> Homo sapiens <400> 643 catccaatcg cagatggagg gactc 25 <210> 644 <211> 25 <212> DNA <213> Homo sapiens <400> 644 gggactcctg acatagccag ctgct 25 <210> 645 <211> 25 <212> DNA <213> Homo sapiens <400> 645 atagccagct gctgtgaaat aatgg 25 <210> 646 <211> 25 <212> DNA <213> Homo sapiens <400> 646 tgctatggag gacttgggag atctt 25 <210> 647 <211> 25 <212> DNA <213> Homo sapiens <400> 647 gtagctgctt gtctcctact atacc 25 <210> 648 <211> 25 <212> DNA <213> Homo sapiens <400> 648 ctcctactat acctgtctga cacaa 25 <210> 649 <211> 25 <212> DNA <213> Homo sapiens <400> 649 ctatacctgt ctgacacaat atcac 25 <210> 650 <211> 25 <212> DNA <213> Homo sapiens <400> 650 gacacaatat caccagagca agcca 25 <210> 651 <211> 25 <212> DNA <213> Homo sapiens <400> 651 ggatccgggg caatacagac catca 25 <210> 652 <211> 25 <212> DNA <213> Homo sapiens <400> 652 aagcaataca attatcttca tgagt 25 <210> 653 <211> 25 <212> DNA <213> Homo sapiens <400> 653 tttcgggaca aattagctgc acatc 25 <210> 654 <211> 25 <212> DNA <213> Homo sapiens <400> 654 ttagctgcac atctatcatc aagag 25 <210> 655 <211> 25 <212> DNA <213> Homo sapiens <400> 655 ctatcatcaa gagattcaca atcaa 25 <210> 656 <211> 25 <212> DNA <213> Homo sapiens <400> 656 tcaagagatt cacaatcaag atctg 25 <210> 657 <211> 25 <212> DNA <213> Homo sapiens <400> 657 gattcacaat caagatctgt atcaa 25 <210> 658 <211> 25 <212> DNA <213> Homo sapiens <400> 658 caatcaagat ctgtatcaag ataaa 25 <210> 659 <211> 25 <212> DNA <213> Homo sapiens <400> 659 tatatgacca tgtctgaaat gtcag 25 <210> 660 <211> 25 <212> DNA <213> Homo sapiens <400> 660 ttatcaactt gaatgtaaat gtaca 25 <210> 661 <211> 25 <212> DNA <213> Homo sapiens <400> 661 tggccttatt gcatcccaaa ccacg 25 <210> 662 <211> 25 <212> DNA <213> Homo sapiens <400> 662 tattgcatcc caaaccacgc ctctt 25 <210> 663 <211> 25 <212> DNA <213> Homo sapiens <400> 663 ggctgcctcc cttgtggcag caacg 25 <210> 664 <211> 25 <212> DNA <213> Homo sapiens <400> 664 tgtggcagca acggcacagc taatt 25 <210> 665 <211> 25 <212> DNA <213> Homo sapiens <400> 665 agcaacggca cagctaattc tactc 25 <210> 666 <211> 25 <212> DNA <213> Homo sapiens <400> 666 ggcacagcta attctactca cagtg 25 <210> 667 <211> 25 <212> DNA <213> Homo sapiens <400> 667 gctaattcta ctcacagtgc tttta 25 <210> 668 <211> 25 <212> DNA <213> Homo sapiens <400> 668 aagaggcacc gggaagccgt cctgg 25 <210> 669 <211> 25 <212> DNA <213> Homo sapiens <400> 669 gaagccgtcc tggcgcctgg cagtc 25 <210> 670 <211> 25 <212> DNA <213> Homo sapiens <400> 670 gtttgagatt ctcaaaggag cgagc 25 <210> 671 <211> 25 <212> DNA <213> Homo sapiens <400> 671 gattctcaaa ggagcgagca tgtcg 25 <210> 672 <211> 25 <212> DNA <213> Homo sapiens <400> 672 caaaggagcg agcatgtcgt ggaca 25 <210> 673 <211> 25 <212> DNA <213> Homo sapiens <400> 673 agcgagcatg tcgtggacac acaca 25 <210> 674 <211> 25 <212> DNA <213> Homo sapiens <400> 674 acacacagac tatttttaga ttttc 25 <210> 675 <211> 25 <212> DNA <213> Homo sapiens <400> 675 cttttgcctt ttgcaaccag gaaca 25 <210> 676 <211> 25 <212> DNA <213> Homo sapiens <400> 676 tgcaaaaact ctttgagagg gtagg 25 <210> 677 <211> 25 <212> DNA <213> Homo sapiens <400> 677 gaaacaacca tgtcatttca gaagt 25 <210> 678 <211> 25 <212> DNA <213> Homo sapiens <400> 678 accatgtcat ttcagaagtt agttt 25 <210> 679 <211> 25 <212> DNA <213> Homo sapiens <400> 679 attgttctca gaatccctta acagt 25 <210> 680 <211> 25 <212> DNA <213> Homo sapiens <400> 680 ctcagaatcc cttaacagtt gtatt 25 <210> 681 <211> 25 <212> DNA <213> Homo sapiens <400> 681 ggagcaaaag ctcatttctg aagag 25 <210> 682 <211> 25 <212> DNA <213> Homo sapiens <400> 682 catttctgaa gaggacttgt tgcgg 25 <210> 683 <211> 25 <212> DNA <213> Homo sapiens <400> 683 gaaacacaaa cttgaacagc tacgg 25 <210> 684 <211> 25 <212> DNA <213> Homo sapiens <400> 684 caaacttgaa cagctacgga actct 25 <210> 685 <211> 25 <212> DNA <213> Homo sapiens <400> 685 gctacggaac tcttgtgcgt aagga 25 <210> 686 <211> 25 <212> DNA <213> Homo sapiens <400> 686 aacgattcct tctaacagaa atgtc 25 <210> 687 <211> 25 <212> DNA <213> Homo sapiens <400> 687 tccttctaac agaaatgtcc tgagc 25 <210> 688 <211> 25 <212> DNA <213> Homo sapiens <400> 688 cctgagcaat cacctatgaa cttgt 25 <210> 689 <211> 25 <212> DNA <213> Homo sapiens <400> 689 caatcaccta tgaacttgtt tcaaa 25 <210> 690 <211> 25 <212> DNA <213> Homo sapiens <400> 690 cctatgaact tgtttcaaat gcatg 25 <210> 691 <211> 25 <212> DNA <213> Homo sapiens <400> 691 aacttgtttc aaatgcatga tcaaa 25 <210> 692 <211> 25 <212> DNA <213> Homo sapiens <400> 692 gatcaaatgc aacctcacaa ccttg 25 <210> 693 <211> 25 <212> DNA <213> Homo sapiens <400> 693 atgcaacctc acaaccttgg ctgag 25 <210> 694 <211> 25 <212> DNA <213> Homo sapiens <400> 694 cctcacaacc ttggctgagt cttga 25 <210> 695 <211> 25 <212> DNA <213> Homo sapiens <400> 695 ataatgtaaa ctgcctcaaa ttgga 25 <210> 696 <211> 25 <212> DNA <213> Homo sapiens <400> 696 taaactgcct caaattggac tttgg 25 <210> 697 <211> 25 <212> DNA <213> Homo sapiens <400> 697 gcctcaaatt ggactttggg cataa 25 <210> 698 <211> 25 <212> DNA <213> Homo sapiens <400> 698 acacaatgtt tctctgtaaa tattg 25 <210> 699 <211> 25 <212> DNA <213> Homo sapiens <400> 699 tgtttctctg taaatattgc catta 25 <210> 700 <211> 25 <212> DNA <213> Homo sapiens <400> 700 aatattgcca ttaaatgtaa ataac 25 <210> 701 <211> 25 <212> DNA <213> Homo sapiens <400> 701 gtgaactctg actgtatgag atgtt 25 <210> 702 <211> 25 <212> DNA <213> Homo sapiens <400> 702 aactctgact gtatgagatg ttaaa 25 <210> 703 <211> 25 <212> DNA <213> Homo sapiens <400> 703 caaacactta cagaattatg aagag 25 <210> 704 <211> 25 <212> DNA <213> Homo sapiens <400> 704 tgtttaacat ttcctcagtc aagtt 25 <210> 705 <211> 25 <212> DNA <213> Homo sapiens <400> 705 ttaacatttc ctcagtcaag ttcag 25 <210> 706 <211> 25 <212> DNA <213> Homo sapiens <400> 706 acatttcctc agtcaagttc agagt 25 <210> 707 <211> 25 <212> DNA <213> Homo sapiens <400> 707 cctcagtcaa gttcagagtc ttcag 25 <210> 708 <211> 25 <212> DNA <213> Homo sapiens <400> 708 cagtcaagtt cagagtcttc agaga 25 <210> 709 <211> 25 <212> DNA <213> Homo sapiens <400> 709 tcaagttcag agtcttcaga gactt 25 <210> 710 <211> 25 <212> DNA <213> Homo sapiens <400> 710 tcagagtctt cagagacttc gtaat 25 <210> 711 <211> 25 <212> DNA <213> Homo sapiens <400> 711 gagtcttcag agacttcgta attaa 25 <210> 712 <211> 25 <212> DNA <213> Homo sapiens <400> 712 tcttcagaga cttcgtaatt aaagg 25 <210> 713 <211> 25 <212> DNA <213> Homo sapiens <400> 713 agacatcatc aagtggagag aaatc 25 <210> 714 <211> 25 <212> DNA <213> Homo sapiens <400> 714 attttcccat ttggactgta actga 25 <210> 715 <211> 25 <212> DNA <213> Homo sapiens <400> 715 ttcccatttg gactgtaact gactg 25 <210> 716 <211> 25 <212> DNA <213> Homo sapiens <400> 716 ccatttggac tgtaactgac tgcct 25 <210> 717 <211> 25 <212> DNA <213> Homo sapiens <400> 717 ggactgtaac tgactgcctt gctaa 25 <210> 718 <211> 25 <212> DNA <213> Homo sapiens <400> 718 ctgtaactga ctgccttgct aaaag 25 <210> 719 <211> 25 <212> DNA <213> Homo sapiens <400> 719 taactgactg ccttgctaaa agatt 25 <210> 720 <211> 25 <212> DNA <213> Homo sapiens <400> 720 actgccttgc taaaagatta tagaa 25 <210> 721 <211> 25 <212> DNA <213> Homo sapiens <400> 721 caggcaagga ccagctgccc attct 25 <210> 722 <211> 25 <212> DNA <213> Homo sapiens <400> 722 aggaccagct gcccattctg agccc 25 <210> 723 <211> 25 <212> DNA <213> Homo sapiens <400> 723 agctgcccat tctgagccca gggca 25 <210> 724 <211> 25 <212> DNA <213> Homo sapiens <400> 724 tgagcccagg gcagcctctt caacc 25 <210> 725 <211> 25 <212> DNA <213> Homo sapiens <400> 725 cagggcagcc tcttcaacca ttatt 25 <210> 726 <211> 25 <212> DNA <213> Homo sapiens <400> 726 tggtctaacc tggcttgtca ggaaa 25 <210> 727 <211> 25 <212> DNA <213> Homo sapiens <400> 727 cttccacatt gggcctggct gctct 25 <210> 728 <211> 25 <212> DNA <213> Homo sapiens <400> 728 cattgggcct ggctgctcta ttctg 25 <210> 729 <211> 25 <212> DNA <213> Homo sapiens <400> 729 gcctggctgc tctattctgt accaa 25 <210> 730 <211> 25 <212> DNA <213> Homo sapiens <400> 730 tcaagttctt agcccttgta gcttc 25 <210> 731 <211> 25 <212> DNA <213> Homo sapiens <400> 731 cccatcctct ctctgtggag gccaa 25 <210> 732 <211> 25 <212> DNA <213> Homo sapiens <400> 732 ctctctctgt ggaggccaaa ccaac 25 <210> 733 <211> 25 <212> DNA <213> Homo sapiens <400> 733 ctctttgcca gcagccacaa catgc 25 <210> 734 <211> 25 <212> DNA <213> Homo sapiens <400> 734 gccagcagcc acaacatgca ttgac 25 <210> 735 <211> 25 <212> DNA <213> Homo sapiens <400> 735 agccacaaca tgcattgaca gcggc 25 <210> 736 <211> 25 <212> DNA <213> Homo sapiens <400> 736 cattgacagc ggcacagtga gatat 25 <210> 737 <211> 25 <212> DNA <213> Homo sapiens <400> 737 cagcggcaca gtgagatata actga 25 <210> 738 <211> 25 <212> DNA <213> Homo sapiens <400> 738 ctggctttcc ttctgggctg tctcc 25 <210> 739 <211> 25 <212> DNA <213> Homo sapiens <400> 739 ctgggctgtc tccatctgac cctac 25 <210> 740 <211> 25 <212> DNA <213> Homo sapiens <400> 740 tcaggatgtt tttcactgtc cattg 25 <210> 741 <211> 25 <212> DNA <213> Homo sapiens <400> 741 atatgtcttc tgtataacta ggctg 25 <210> 742 <211> 25 <212> DNA <213> Homo sapiens <400> 742 cttctgtata actaggctgt taacc 25 <210> 743 <211> 25 <212> DNA <213> Homo sapiens <400> 743 tataactagg ctgttaacct atgat 25 <210> 744 <211> 25 <212> DNA <213> Homo sapiens <400> 744 gttaacctat gattgtaaag tagct 25 <210> 745 <211> 25 <212> DNA <213> Homo sapiens <400> 745 agtgttctcg gggatgcata gattc 25 <210> 746 <211> 25 <212> DNA <213> Homo sapiens <400> 746 tagattcatc attttctcca cctta 25 <210> 747 <211> 25 <212> DNA <213> Homo sapiens <400> 747 atttaagtct gtccattatc tatat 25 <210> 748 <211> 25 <212> DNA <213> Homo sapiens <400> 748 gtctgtccat tatctatata gtcct 25 <210> 749 <211> 25 <212> DNA <213> Homo sapiens <400> 749 tatgcataat cagacaagcg taatg 25 <210> 750 <211> 25 <212> DNA <213> Homo sapiens <400> 750 taatcagaca agcgtaatgc ataat 25 <210> 751 <211> 25 <212> DNA <213> Homo sapiens <400> 751 gcataatcag acaagcttga atatt 25 <210> 752 <211> 25 <212> DNA <213> Homo sapiens <400> 752 tgtttttgca ccagacgaac agtga 25 <210> 753 <211> 25 <212> DNA <213> Homo sapiens <400> 753 gctatacata tgtgcagaag gttac 25 <210> 754 <211> 25 <212> DNA <213> Homo sapiens <400> 754 agattgctct gccaagatac ttgaa 25 <210> 755 <211> 25 <212> DNA <213> Homo sapiens <400> 755 ctctgccaag atacttgaac actgt 25 <210> 756 <211> 25 <212> DNA <213> Homo sapiens <400> 756 caagatactt gaacactgtg tttta 25 <210> 757 <211> 25 <212> DNA <213> Homo sapiens <400> 757 tgtgattcaa acttctgtgt actgg 25 <210> 758 <211> 25 <212> DNA <213> Homo sapiens <400> 758 gcacccattg tgattgtgga agata 25 <210> 759 <211> 25 <212> DNA <213> Homo sapiens <400> 759 catagagtat agctctgtag tggaa 25 <210> 760 <211> 25 <212> DNA <213> Homo sapiens <400> 760 gtatagctct gtagtggaat atgtc 25 <210> 761 <211> 25 <212> DNA <213> Homo sapiens <400> 761 ggcgctgtga ttcccgacca ctggc 25 <210> 762 <211> 25 <212> DNA <213> Homo sapiens <400> 762 gtgattcccg accactggct ggacc 25 <210> 763 <211> 25 <212> DNA <213> Homo sapiens <400> 763 cttcgcttcc ctcggtttat tcgag 25 <210> 764 <211> 25 <212> DNA <213> Homo sapiens <400> 764 ttccctcggt ttattcgagt ccgtg 25 <210> 765 <211> 25 <212> DNA <213> Homo sapiens <400> 765 cggtttattc gagtccgtga agaca 25 <210> 766 <211> 25 <212> DNA <213> Homo sapiens <400> 766 gaagacaagc agccggagca ggcca 25 <210> 767 <211> 25 <212> DNA <213> Homo sapiens <400> 767 gcctgtttgt accggaagca aagtc 25 <210> 768 <211> 25 <212> DNA <213> Homo sapiens <400> 768 cagattcaga accaacaagg cgagg 25 <210> 769 <211> 25 <212> DNA <213> Homo sapiens <400> 769 cagaaccaac aaggcgagga ctcag 25 <210> 770 <211> 25 <212> DNA <213> Homo sapiens <400> 770 ggcgaggact caggctctga ccctg 25 <210> 771 <211> 25 <212> DNA <213> Homo sapiens <400> 771 gactcaggct ctgaccctga agata 25 <210> 772 <211> 25 <212> DNA <213> Homo sapiens <400> 772 ctcctagggc ctgggtacag ggcat 25 <210> 773 <211> 25 <212> DNA <213> Homo sapiens <400> 773 tgagttggac ggaccccagg gttat 25 <210> 774 <211> 25 <212> DNA <213> Homo sapiens <400> 774 cctttgcttt ttagcaaatc tgctg 25 <210> 775 <211> 25 <212> DNA <213> Homo sapiens <400> 775 ctttttagca aatctgctgt ggcag 25 <210> 776 <211> 25 <212> DNA <213> Homo sapiens <400> 776 tctgctgtgg caggctgtgg atttt 25 <210> 777 <211> 25 <212> DNA <213> Homo sapiens <400> 777 gcttactccg gagtctggga ttcat 25 <210> 778 <211> 25 <212> DNA <213> Homo sapiens <400> 778 tccggagtct gggattcatc ccgtc 25 <210> 779 <211> 25 <212> DNA <213> Homo sapiens <400> 779 gtctgggatt catcccgtca tttct 25 <210> 780 <211> 25 <212> DNA <213> Homo sapiens <400> 780 gattcatccc gtcatttctt tcaat 25 <210> 781 <211> 25 <212> DNA <213> Homo sapiens <400> 781 catcaggttc tcacacccac gtagt 25 <210> 782 <211> 25 <212> DNA <213> Homo sapiens <400> 782 gttctcacac ccacgtagta tgctg 25 <210> 783 <211> 25 <212> DNA <213> Homo sapiens <400> 783 attttttcgt gtgtgcaaat gtgaa 25 <210> 784 <211> 25 <212> DNA <213> Homo sapiens <400> 784 tgaacatttg aacatttcag ttagg 25 <210> 785 <211> 25 <212> DNA <213> Homo sapiens <400> 785 acatttgaac atttcagtta ggtac 25 <210> 786 <211> 25 <212> DNA <213> Homo sapiens <400> 786 gaacatttca gttaggtact gagcc 25 <210> 787 <211> 25 <212> DNA <213> Homo sapiens <400> 787 ttcagttagg tactgagcca gaaaa 25 <210> 788 <211> 25 <212> DNA <213> Homo sapiens <400> 788 taggtactga gccagaaaag gtaat 25 <210> 789 <211> 25 <212> DNA <213> Homo sapiens <400> 789 aagttttcag aaagcttctg tgctt 25 <210> 790 <211> 25 <212> DNA <213> Homo sapiens <400> 790 ttttcagaaa gcttctgtgc ttaga 25 <210> 791 <211> 25 <212> DNA <213> Homo sapiens <400> 791 gtattttgta gctggaagaa taaga 25 <210> 792 <211> 25 <212> DNA <213> Homo sapiens <400> 792 atcaaggctt acatgtttca ggcca 25 <210> 793 <211> 25 <212> DNA <213> Homo sapiens <400> 793 gcttacatgt ttcaggccac ttggt 25 <210> 794 <211> 25 <212> DNA <213> Homo sapiens <400> 794 tttcaggcca cttggtcact ggttc 25 <210> 795 <211> 25 <212> DNA <213> Homo sapiens <400> 795 acttggtcac tggttcctga attta 25 <210> 796 <211> 25 <212> DNA <213> Homo sapiens <400> 796 tggtcactgg ttcctgaatt tagaa 25 <210> 797 <211> 25 <212> DNA <213> Homo sapiens <400> 797 tcactggttc ctgaatttag aaaac 25 <210> 798 <211> 25 <212> DNA <213> Homo sapiens <400> 798 ttaactgtgg cacatgtttt gatca 25 <210> 799 <211> 25 <212> DNA <213> Homo sapiens <400> 799 gtggcacatg ttttgatcag aaagg 25 <210> 800 <211> 25 <212> DNA <213> Homo sapiens <400> 800 acgtctaatc atactgaatt gtaaa 25 <210> 801 <211> 25 <212> DNA <213> Homo sapiens <400> 801 gaggaacaca gcagccatgg tgtgc 25 <210> 802 <211> 25 <212> DNA <213> Homo sapiens <400> 802 cacagcagcc atggtgtgct ctttg 25 <210> 803 <211> 25 <212> DNA <213> Homo sapiens <400> 803 ggtgtgctct ttggagaata gagat 25 <210> 804 <211> 25 <212> DNA <213> Homo sapiens <400> 804 gtgtggatcc tgaggaaaga cttgg 25 <210> 805 <211> 25 <212> DNA <213> Homo sapiens <400> 805 gaagagacca gcatgtcttc agtag 25 <210> 806 <211> 25 <212> DNA <213> Homo sapiens <400> 806 accagcatgt cttcagtagc caaac 25 <210> 807 <211> 25 <212> DNA <213> Homo sapiens <400> 807 atgtcttcag tagccaaact acttc 25 <210> 808 <211> 25 <212> DNA <213> Homo sapiens <400> 808 tcagtagcca aactacttct tgagc 25 <210> 809 <211> 25 <212> DNA <213> Homo sapiens <400> 809 gtttgcttga ggtggtaagg ctttg 25 <210> 810 <211> 25 <212> DNA <213> Homo sapiens <400> 810 ccctgttgca ggcaaaagga gtaat 25 <210> 811 <211> 25 <212> DNA <213> Homo sapiens <400> 811 taaggtttca tcacccattt agcac 25 <210> 812 <211> 25 <212> DNA <213> Homo sapiens <400> 812 acccatttag cactgctttt ctgaa 25 <210> 813 <211> 25 <212> DNA <213> Homo sapiens <400> 813 ttctgaagac ttcagttttg ttaag 25 <210> 814 <211> 25 <212> DNA <213> Homo sapiens <400> 814 ttagttttac tgctttgact ggtgg 25 <210> 815 <211> 25 <212> DNA <213> Homo sapiens <400> 815 ctttgactgg tgggtcctct agatg 25 <210> 816 <211> 25 <212> DNA <213> Homo sapiens <400> 816 ggtcctctag atgcaaaact gagtg 25 <210> 817 <211> 25 <212> DNA <213> Homo sapiens <400> 817 ggacctttat ctggatatgg tccta 25 <210> 818 <211> 25 <212> DNA <213> Homo sapiens <400> 818 ttatctggat atggtcctat aggtt 25 <210> 819 <211> 25 <212> DNA <213> Homo sapiens <400> 819 ggatatggtc ctataggtta ttctg 25 <210> 820 <211> 25 <212> DNA <213> Homo sapiens <400> 820 ggtcctatag gttattctga aataa 25 <210> 821 <211> 25 <212> DNA <213> Homo sapiens <400> 821 tggactaatg ttactgtcca agttc 25 <210> 822 <211> 25 <212> DNA <213> Homo sapiens <400> 822 caagttcttt ctcaagaaac cacat 25 <210> 823 <211> 25 <212> DNA <213> Homo sapiens <400> 823 ctttctcaag aaaccacatc tggtt 25 <210> 824 <211> 25 <212> DNA <213> Homo sapiens <400> 824 caagaaacca catctggttc agaag 25 <210> 825 <211> 25 <212> DNA <213> Homo sapiens <400> 825 caagttggac tctttgaact ctgtt 25 <210> 826 <211> 25 <212> DNA <213> Homo sapiens <400> 826 tttgaactct gttgctgtct gagca 25 <210> 827 <211> 25 <212> DNA <213> Homo sapiens <400> 827 ctctgttgct gtctgagcaa tcgtg 25 <210> 828 <211> 25 <212> DNA <213> Homo sapiens <400> 828 tgctgtctga gcaatcgtgg tgcct 25 <210> 829 <211> 25 <212> DNA <213> Homo sapiens <400> 829 ctgagcaatc gtggtgccta gactt 25 <210> 830 <211> 25 <212> DNA <213> Homo sapiens <400> 830 ggtgcctaga ctttgcattc cttgt 25 <210> 831 <211> 25 <212> DNA <213> Homo sapiens <400> 831 ttgcattcct tgttctgttg acctg 25 <210> 832 <211> 25 <212> DNA <213> Homo sapiens <400> 832 tccttgttct gttgacctgc ataca 25 <210> 833 <211> 25 <212> DNA <213> Homo sapiens <400> 833 ctatataggc tgtgaaaacg cactt 25 <210> 834 <211> 25 <212> DNA <213> Homo sapiens <400> 834 gcagcatgct gggacaatta tttga 25 <210> 835 <211> 25 <212> DNA <213> Homo sapiens <400> 835 ctctatggat tctgacagag atttc 25 <210> 836 <211> 25 <212> DNA <213> Homo sapiens <400> 836 ttgtcagttt cattttaacg agtgt 25 <210> 837 <211> 25 <212> DNA <213> Homo sapiens <400> 837 atgacattga tggcattgtg aagcc 25 <210> 838 <211> 25 <212> DNA <213> Homo sapiens <400> 838 tgaagcctgt tactttgtgt cactt 25 <210> 839 <211> 25 <212> DNA <213> Homo sapiens <400> 839 ctgttacttt gtgtcacttc ctgat 25 <210> 840 <211> 25 <212> DNA <213> Homo sapiens <400> 840 gctgcttccc tgtggcaact gtggc 25 <210> 841 <211> 25 <212> DNA <213> Homo sapiens <400> 841 gagtgatggt tctggctgat ttgtt 25 <210> 842 <211> 25 <212> DNA <213> Homo sapiens <400> 842 gtgatggttc tggctgattt gtttg 25 <210> 843 <211> 25 <212> DNA <213> Homo sapiens <400> 843 atggttctgg ctgatttgtt tgtga 25 <210> 844 <211> 25 <212> DNA <213> Homo sapiens <400> 844 gatttgtttg tgatgttgtt agccg 25 <210> 845 <211> 25 <212> DNA <213> Homo sapiens <400> 845 tttgtttgtg atgttgttag ccgcc 25 <210> 846 <211> 25 <212> DNA <213> Homo sapiens <400> 846 ttgtttgtga tgttgttagc cgcct 25 <210> 847 <211> 25 <212> DNA <213> Homo sapiens <400> 847 tttgtgatgt tgttagccgc ctgtg 25 <210> 848 <211> 25 <212> DNA <213> Homo sapiens <400> 848 tgtgatgttg ttagccgcct gtgag 25 <210> 849 <211> 25 <212> DNA <213> Homo sapiens <400> 849 tgatgttgtt agccgcctgt gaggg 25 <210> 850 <211> 25 <212> DNA <213> Homo sapiens <400> 850 catgcttcac aatgttaagt tatat 25 <210> 851 <211> 25 <212> DNA <213> Homo sapiens <400> 851 gcaacagcaa acaggtgcta atttg 25 <210> 852 <211> 25 <212> DNA <213> Homo sapiens <400> 852 gcagtgtctg tgttttgaaa gaata 25 <210> 853 <211> 25 <212> DNA <213> Homo sapiens <400> 853 cagtttgtag tgccactgtt gtttt 25 <210> 854 <211> 25 <212> DNA <213> Homo sapiens <400> 854 gttgtacttg gaattcttag tcaca 25 <210> 855 <211> 25 <212> DNA <213> Homo sapiens <400> 855 cttggaattc ttagtcacaa aatat 25 <210> 856 <211> 25 <212> DNA <213> Homo sapiens <400> 856 gtttaaagtc tcagtttctt gcttg 25 <210> 857 <211> 25 <212> DNA <213> Homo sapiens <400> 857 agtctcagtt tcttgcttgg ggaac 25 <210> 858 <211> 25 <212> DNA <213> Homo sapiens <400> 858 ttgcttgggg aacttgtgtc cctaa 25 <210> 859 <211> 25 <212> DNA <213> Homo sapiens <400> 859 ggggaacttg tgtccctaat gtgtt 25 <210> 860 <211> 25 <212> DNA <213> Homo sapiens <400> 860 cttgtgtccc taatgtgtta gattg 25 <210> 861 <211> 25 <212> DNA <213> Homo sapiens <400> 861 tagattgcta gattgctaag gagct 25 <210> 862 <211> 25 <212> DNA <213> Homo sapiens <400> 862 tttagactta tggttgttaa aaatg 25 <210> 863 <211> 25 <212> DNA <213> Homo sapiens <400> 863 gtcatctcaa gtcaagtcac tggtc 25 <210> 864 <211> 25 <212> DNA <213> Homo sapiens <400> 864 caagtcactg gtctgtttgc atttg 25 <210> 865 <211> 25 <212> DNA <213> Homo sapiens <400> 865 actggtctgt ttgcatttga tacat 25 <210> 866 <211> 25 <212> DNA <213> Homo sapiens <400> 866 gcatttgata catttttgta ctaac 25 <210> 867 <211> 25 <212> DNA <213> Homo sapiens <400> 867 gatacatttt tgtactaact agcat 25 <210> 868 <211> 25 <212> DNA <213> Homo sapiens <400> 868 tttttgtact aactagcatt gtaaa 25 <210> 869 <211> 25 <212> DNA <213> Homo sapiens <400> 869 attagaaatt acctgtggat atttg 25 <210> 870 <211> 25 <212> DNA <213> Homo sapiens <400> 870 ttgatgctat ttgggttatg atggg 25 <210> 871 <211> 25 <212> DNA <213> Homo sapiens <400> 871 ctatttgggt tatgatgggg caaga 25 <210> 872 <211> 25 <212> DNA <213> Homo sapiens <400> 872 tttaaatcta gcccagtata aacat 25 <210> 873 <211> 25 <212> DNA <213> Homo sapiens <400> 873 ttctgagcac tcaactcatg tttgg 25 <210> 874 <211> 25 <212> DNA <213> Homo sapiens <400> 874 aagtgtgact tcgaggacca aagaa 25 <210> 875 <211> 25 <212> DNA <213> Homo sapiens <400> 875 gacttcgagg accaaagaaa ttgtc 25 <210> 876 <211> 25 <212> DNA <213> Homo sapiens <400> 876 cagctataca tttatcttta tgaac 25 <210> 877 <211> 25 <212> DNA <213> Homo sapiens <400> 877 taatgactcg ttgttctaac atttc 25 <210> 878 <211> 25 <212> DNA <213> Homo sapiens <400> 878 acatttccta gaagtgttct tataa 25 <210> 879 <211> 25 <212> DNA <213> Homo sapiens <400> 879 atccacaggc tgttgtctta ttagt 25 <210> 880 <211> 25 <212> DNA <213> Homo sapiens <400> 880 aggctgttgt cttattagta aatgc 25 <210> 881 <211> 25 <212> DNA <213> Homo sapiens <400> 881 caaagtaatg actttgtctg tttta 25 <210> 882 <211> 25 <212> DNA <213> Homo sapiens <400> 882 actctagtct ttagtacttc aaaat 25 <210> 883 <211> 25 <212> DNA <213> Homo sapiens <400> 883 tcaaaattac cttttcatat ccatg 25 <210> 884 <211> 25 <212> DNA <213> Homo sapiens <400> 884 tttcatatcc atgatcttga gtcca 25 <210> 885 <211> 25 <212> DNA <213> Homo sapiens <400> 885 atccatgatc ttgagtccat ttggg 25 <210> 886 <211> 25 <212> DNA <213> Homo sapiens <400> 886 gatcttgagt ccatttgggg gattt 25 <210> 887 <211> 25 <212> DNA <213> Homo sapiens <400> 887 atgtatttca atacactgtt caaaa 25 <210> 888 <211> 25 <212> DNA <213> Homo sapiens <400> 888 gagtatgtat gttcctgaag ttggt 25 <210> 889 <211> 25 <212> DNA <213> Homo sapiens <400> 889 gtatgttcct gaagttggtc ctatt 25 <210> 890 <211> 25 <212> DNA <213> Homo sapiens <400> 890 tccttcattg ggtactcctg aaatc 25 <210> 891 <211> 25 <212> DNA <213> Homo sapiens <400> 891 attgggtact cctgaaatca gacat 25 <210> 892 <211> 25 <212> DNA <213> Homo sapiens <400> 892 tactcctgaa atcagacatg ttcct 25 <210> 893 <211> 25 <212> DNA <213> Homo sapiens <400> 893 tgaaatcaga catgttcctg tagaa 25 <210> 894 <211> 25 <212> DNA <213> Homo sapiens <400> 894 tttctatgca cctatcaaga atcaa 25 <210> 895 <211> 25 <212> DNA <213> Homo sapiens <400> 895 cttcagcaat tctaatccac tttgg 25 <210> 896 <211> 25 <212> DNA <213> Homo sapiens <400> 896 tttttacatc taaagcaata gacta 25 <210> 897 <211> 25 <212> DNA <213> Homo sapiens <400> 897 catctaaagc aatagactag aactg 25 <210> 898 <211> 25 <212> DNA <213> Homo sapiens <400> 898 agaactgaat tatcttctac atagt 25 <210> 899 <211> 25 <212> DNA <213> Homo sapiens <400> 899 acatagtaaa atcacaattg tggaa 25 <210> 900 <211> 25 <212> DNA <213> Homo sapiens <400> 900 cacaattgtg gaattctggt gatat 25 <210> 901 <211> 25 <212> DNA <213> Homo sapiens <400> 901 attctggtga tattaaggtg aaata 25 <210> 902 <211> 25 <212> DNA <213> Homo sapiens <400> 902 ataggcagct gtagatcttg atctt 25 <210> 903 <211> 25 <212> DNA <213> Homo sapiens <400> 903 agctgtagat cttgatcttc caggt 25 <210> 904 <211> 25 <212> DNA <213> Homo sapiens <400> 904 agatcttgat cttccaggta cccca 25 <210> 905 <211> 25 <212> DNA <213> Homo sapiens <400> 905 tgatcttcca ggtaccccat gtacc 25 <210> 906 <211> 25 <212> DNA <213> Homo sapiens <400> 906 taatactgtc aaattgccac gatct 25 <210> 907 <211> 25 <212> DNA <213> Homo sapiens <400> 907 tgtcaaattg ccacgatctc actaa 25 <210> 908 <211> 25 <212> DNA <213> Homo sapiens <400> 908 attgccacga tctcactaaa ggatt 25 <210> 909 <211> 25 <212> DNA <213> Homo sapiens <400> 909 aaggatttct atttgctgtc agtta 25 <210> 910 <211> 25 <212> DNA <213> Homo sapiens <400> 910 aacttgtcat tctagatgtt ctgtt 25 <210> 911 <211> 25 <212> DNA <213> Homo sapiens <400> 911 tcattctaga tgttctgttg ccagt 25 <210> 912 <211> 25 <212> DNA <213> Homo sapiens <400> 912 tagatgttct gttgccagta tcaaa 25 <210> 913 <211> 25 <212> DNA <213> Homo sapiens <400> 913 cttccaggct gtcacgggca agcga 25 <210> 914 <211> 25 <212> DNA <213> Homo sapiens <400> 914 caagcgagca cagctccgtg ccaaa 25 <210> 915 <211> 25 <212> DNA <213> Homo sapiens <400> 915 tgccaaagct aactaatttg ctaat 25 <210> 916 <211> 25 <212> DNA <213> Homo sapiens <400> 916 ctaatttgct aatcactgat tttgc 25 <210> 917 <211> 25 <212> DNA <213> Homo sapiens <400> 917 tgctaatcac tgattttgca aagct 25 <210> 918 <211> 25 <212> DNA <213> Homo sapiens <400> 918 tcactgattt tgcaaagctt gttgt 25 <210> 919 <211> 25 <212> DNA <213> Homo sapiens <400> 919 tgtggctgga caggtttgcc atcag 25 <210> 920 <211> 25 <212> DNA <213> Homo sapiens <400> 920 tggacaggtt tgccatcaga gtgga 25 <210> 921 <211> 25 <212> DNA <213> Homo sapiens <400> 921 tctgaagtcc ttgcaagacg ctgat 25 <210> 922 <211> 25 <212> DNA <213> Homo sapiens <400> 922 cgctgatgct caagctgttg acata 25 <210> 923 <211> 25 <212> DNA <213> Homo sapiens <400> 923 tgctcaagct gttgacatac tcatt 25 <210> 924 <211> 25 <212> DNA <213> Homo sapiens <400> 924 agctgttgac atactcattg cctac 25 <210> 925 <211> 25 <212> DNA <213> Homo sapiens <400> 925 tgacatactc attgcctact ttaac 25 <210> 926 <211> 25 <212> DNA <213> Homo sapiens <400> 926 tgcctacttt aacacctgtc agaga 25 <210> 927 <211> 25 <212> DNA <213> Homo sapiens <400> 927 cacctgtcag agaaacgtga tatgg 25 <210> 928 <211> 25 <212> DNA <213> Homo sapiens <400> 928 tcgttcatag acttctgtaa aatgc 25 <210> 929 <211> 25 <212> DNA <213> Homo sapiens <400> 929 atagacttct gtaaaatgca agata 25 <210> 930 <211> 25 <212> DNA <213> Homo sapiens <400> 930 tgtgcgccgc tcagatgtgg cctgg 25 <210> 931 <211> 25 <212> DNA <213> Homo sapiens <400> 931 ccgctcagat gtggcctgga acttt 25 <210> 932 <211> 25 <212> DNA <213> Homo sapiens <400> 932 tgagaagttc ctcatagggc cggag 25 <210> 933 <211> 25 <212> DNA <213> Homo sapiens <400> 933 cttcccaacc atcaacattg agcct 25 <210> 934 <211> 25 <212> DNA <213> Homo sapiens <400> 934 caacattgag cctgacatca agcgc 25 <210> 935 <211> 25 <212> DNA <213> Homo sapiens <400> 935 tgagcctgac atcaagcgcc tcctt 25 <210> 936 <211> 25 <212> DNA <213> Homo sapiens <400> 936 caagcgcctc cttaaagttg ccata 25 <210> 937 <211> 25 <212> DNA <213> Homo sapiens <400> 937 cctccttaaa gttgccatat agatg 25 <210> 938 <211> 25 <212> DNA <213> Homo sapiens <400> 938 agtcctgagg agccttagga tgcag 25 <210> 939 <211> 25 <212> DNA <213> Homo sapiens <400> 939 gaggagcctt aggatgcagc atgcc 25 <210> 940 <211> 25 <212> DNA <213> Homo sapiens <400> 940 ctgctggacc tcagcattcc cttga 25 <210> 941 <211> 25 <212> DNA <213> Homo sapiens <400> 941 cccttgatat cagtcccctt cactg 25 <210> 942 <211> 25 <212> DNA <213> Homo sapiens <400> 942 gattcaactt gggctccaag acttg 25 <210> 943 <211> 25 <212> DNA <213> Homo sapiens <400> 943 gctccaagac ttgggtaagc tctgg 25 <210> 944 <211> 25 <212> DNA <213> Homo sapiens <400> 944 gctctgggcc ttcacagaat gatgg 25 <210> 945 <211> 25 <212> DNA <213> Homo sapiens <400> 945 atgatggcac cttcctaaac cctca 25 <210> 946 <211> 25 <212> DNA <213> Homo sapiens <400> 946 gcgtgaaggg cctggagcca ctctg 25 <210> 947 <211> 25 <212> DNA <213> Homo sapiens <400> 947 agggcctgga gccactctgc tagaa 25 <210> 948 <211> 25 <212> DNA <213> Homo sapiens <400> 948 tggagccact ctgctagaag agacc 25 <210> 949 <211> 25 <212> DNA <213> Homo sapiens <400> 949 cactctgcta gaagagacca ataaa 25 <210> 950 <211> 25 <212> DNA <213> Homo sapiens <400> 950 atattaatga taaccttgtt ggaat 25 <210> 951 <211> 25 <212> DNA <213> Homo sapiens <400> 951 attacctact cttggcagtg ttagg 25 <210> 952 <211> 25 <212> DNA <213> Homo sapiens <400> 952 ctcttggcag tgttagggta tctgt 25 <210> 953 <211> 25 <212> DNA <213> Homo sapiens <400> 953 aatgataagt ctcactcaag atttt 25 <210> 954 <211> 25 <212> DNA <213> Homo sapiens <400> 954 ttttggtgtg ctacaaaagc cttta 25 <210> 955 <211> 25 <212> DNA <213> Homo sapiens <400> 955 aagcctttac aaattatcag tagta 25 <210> 956 <211> 25 <212> DNA <213> Homo sapiens <400> 956 gaatgagccg atattggctt agtgc 25 <210> 957 <211> 25 <212> DNA <213> Homo sapiens <400> 957 tattggctta gtgctcacta gggga 25 <210> 958 <211> 25 <212> DNA <213> Homo sapiens <400> 958 agtgctcact aggggacatg cacat 25 <210> 959 <211> 25 <212> DNA <213> Homo sapiens <400> 959 ggacatgcac atggaagctc acctc 25 <210> 960 <211> 25 <212> DNA <213> Homo sapiens <400> 960 acatggaagc tcacctctaa gaaag 25 <210> 961 <211> 25 <212> DNA <213> Homo sapiens <400> 961 tttttcccac ctgtgaatat gtaaa 25 <210> 962 <211> 25 <212> DNA <213> Homo sapiens <400> 962 ataccaatga cagaacagag atttg 25 <210> 963 <211> 25 <212> DNA <213> Homo sapiens <400> 963 tttgtgtgct catcttaaga gccag 25 <210> 964 <211> 25 <212> DNA <213> Homo sapiens <400> 964 catcttaaga gccagagcca tataa 25 <210> 965 <211> 25 <212> DNA <213> Homo sapiens <400> 965 gagccagagc catataagca tcttg 25 <210> 966 <211> 25 <212> DNA <213> Homo sapiens <400> 966 gccatataag catcttggga aagca 25 <210> 967 <211> 25 <212> DNA <213> Homo sapiens <400> 967 aagcaagttt gaaccagctg ctggt 25 <210> 968 <211> 25 <212> DNA <213> Homo sapiens <400> 968 cagctgctgg tgtaatgtac agtta 25 <210> 969 <211> 25 <212> DNA <213> Homo sapiens <400> 969 aatggagctg tttatggcaa tttaa 25 <210> 970 <211> 25 <212> DNA <213> Homo sapiens <400> 970 attgatgcag tcttctattc taaaa 25 <210> 971 <211> 25 <212> DNA <213> Homo sapiens <400> 971 tatttcttcc aaggatctaa ccaat 25 <210> 972 <211> 25 <212> DNA <213> Homo sapiens <400> 972 ttccaaggat ctaaccaatt tgaat 25 <210> 973 <211> 25 <212> DNA <213> Homo sapiens <400> 973 ggatctaacc aatttgaata tgact 25 <210> 974 <211> 25 <212> DNA <213> Homo sapiens <400> 974 tttgaatatg acttcctact ccaac 25 <210> 975 <211> 25 <212> DNA <213> Homo sapiens <400> 975 tatgacttcc tactccaacg tatca 25 <210> 976 <211> 25 <212> DNA <213> Homo sapiens <400> 976 ctccaacgta tcaccaaaac actga 25 <210> 977 <211> 25 <212> DNA <213> Homo sapiens <400> 977 ttgttagttc acttcagctt aataa 25 <210> 978 <211> 25 <212> DNA <213> Homo sapiens <400> 978 tattgcatat ttgctatgtc ctcag 25 <210> 979 <211> 25 <212> DNA <213> Homo sapiens <400> 979 atatttgcta tgtcctcagt gtacc 25 <210> 980 <211> 25 <212> DNA <213> Homo sapiens <400> 980 gctatgtcct cagtgtacca ctact 25 <210> 981 <211> 25 <212> DNA <213> Homo sapiens <400> 981 ctctaattgt ccattcttgc ttgac 25 <210> 982 <211> 25 <212> DNA <213> Homo sapiens <400> 982 ttgtccattc ttgcttgact ctact 25 <210> 983 <211> 25 <212> DNA <213> Homo sapiens <400> 983 attcttgctt gactctacta ttaag 25 <210> 984 <211> 25 <212> DNA <213> Homo sapiens <400> 984 gcttgactct actattaagt ttgaa 25 <210> 985 <211> 25 <212> DNA <213> Homo sapiens <400> 985 ttaccttcaa agcaagataa ttcta 25 <210> 986 <211> 25 <212> DNA <213> Homo sapiens <400> 986 agcatgctct gtaagttgct tccta 25 <210> 987 <211> 25 <212> DNA <213> Homo sapiens <400> 987 aagttgcttc ctaacatcct tggac 25 <210> 988 <211> 25 <212> DNA <213> Homo sapiens <400> 988 aacatccttg gactgagaaa ttata 25 <210> 989 <211> 25 <212> DNA <213> Homo sapiens <400> 989 aattatactt acttctggca taact 25 <210> 990 <211> 25 <212> DNA <213> Homo sapiens <400> 990 tcgtcactga agaaatcatt cccct 25 <210> 991 <211> 25 <212> DNA <213> Homo sapiens <400> 991 cgcctgctgt tcccgcgctg agcta 25 <210> 992 <211> 25 <212> DNA <213> Homo sapiens <400> 992 tgagctacag gactcccgag tgtga 25 <210> 993 <211> 25 <212> DNA <213> Homo sapiens <400> 993 acaggactcc cgagtgtgag ccgcg 25 <210> 994 <211> 25 <212> DNA <213> Homo sapiens <400> 994 ctcccgagtg tgagccgcgg ttcct 25 <210> 995 <211> 25 <212> DNA <213> Homo sapiens <400> 995 ggttcctctg ttgcagcgca gccgt 25 <210> 996 <211> 25 <212> DNA <213> Homo sapiens <400> 996 tccctgtgct ctctggctct ggact 25 <210> 997 <211> 25 <212> DNA <213> Homo sapiens <400> 997 tgtgactgcg cctggattct gccat 25 <210> 998 <211> 25 <212> DNA <213> Homo sapiens <400> 998 tgcgcctgga ttctgccatt gcgac 25 <210> 999 <211> 25 <212> DNA <213> Homo sapiens <400> 999 ctgccattgc gacacatttt tgtgc 25 <210> 1000 <211> 25 <212> DNA <213> Homo sapiens <400> 1000 ttgcgacaca tttttgtgcc tttca 25 <210> 1001 <211> 25 <212> DNA <213> Homo sapiens <400> 1001 ctgttggaac cgccggcgtt ggctc 25 <210> 1002 <211> 25 <212> DNA <213> Homo sapiens <400> 1002 acttagcgac gccactggcg gcacc 25 <210> 1003 <211> 25 <212> DNA <213> Homo sapiens <400> 1003 ctgcgcccag tgatgtttcc acggt 25 <210> 1004 <211> 25 <212> DNA <213> Homo sapiens <400> 1004 tgcctgtaca cagccgagca gcatt 25 <210> 1005 <211> 25 <212> DNA <213> Homo sapiens <400> 1005 gccgagcagc atttccgttg aagga 25 <210> 1006 <211> 25 <212> DNA <213> Homo sapiens <400> 1006 tgaaggactt gcatccccat tgcgg 25 <210> 1007 <211> 25 <212> DNA <213> Homo sapiens <400> 1007 acttgcatcc ccattgcggg cagtg 25 <210> 1008 <211> 25 <212> DNA <213> Homo sapiens <400> 1008 atccccattg cgggcagtgc tggac 25 <210> 1009 <211> 25 <212> DNA <213> Homo sapiens <400> 1009 attgcgggca gtgctggacg tgtcc 25 <210> 1010 <211> 25 <212> DNA <213> Homo sapiens <400> 1010 cagtgccatc tataaaatgg ggata 25 <210> 1011 <211> 25 <212> DNA <213> Homo sapiens <400> 1011 aatagcacta cctaccaagt gctgt 25 <210> 1012 <211> 25 <212> DNA <213> Homo sapiens <400> 1012 actacctacc aagtgctgtg aggct 25 <210> 1013 <211> 25 <212> DNA <213> Homo sapiens <400> 1013 taccaagtgc tgtgaggctc aaatg 25 <210> 1014 <211> 25 <212> DNA <213> Homo sapiens <400> 1014 ttgccttaaa actatagtcc tatac 25 <210> 1015 <211> 25 <212> DNA <213> Homo sapiens <400> 1015 tatagtccta tacaaaaatt agctg 25 <210> 1016 <211> 25 <212> DNA <213> Homo sapiens <400> 1016 tatatactgc tgtatcatgc cagga 25 <210> 1017 <211> 25 <212> DNA <213> Homo sapiens <400> 1017 atttatcagc attcccaagg gagct 25 <210> 1018 <211> 25 <212> DNA <213> Homo sapiens <400> 1018 gggagcttgc acggtactga ccgag 25 <210> 1019 <211> 25 <212> DNA <213> Homo sapiens <400> 1019 ttgcacggta ctgaccgagt gctga 25 <210> 1020 <211> 25 <212> DNA <213> Homo sapiens <400> 1020 ggtactgacc gagtgctgag actac 25 <210> 1021 <211> 25 <212> DNA <213> Homo sapiens <400> 1021 gaccgagtgc tgagactact ggtat 25 <210> 1022 <211> 25 <212> DNA <213> Homo sapiens <400> 1022 gaatattctc agcacaggaa tgagg 25 <210> 1023 <211> 25 <212> DNA <213> Homo sapiens <400> 1023 cacaggaatg aggcttcctt ggttt 25 <210> 1024 <211> 25 <212> DNA <213> Homo sapiens <400> 1024 gcttccttgg tttccatgtc tgtaa 25 <210> 1025 <211> 25 <212> DNA <213> Homo sapiens <400> 1025 ttggtttcca tgtctgtaag ggtta 25 <210> 1026 <211> 25 <212> DNA <213> Homo sapiens <400> 1026 agggttactg atcacttacc ttctt 25 <210> 1027 <211> 25 <212> DNA <213> Homo sapiens <400> 1027 tctctttcag acttgaatct gtaga 25 <210> 1028 <211> 25 <212> DNA <213> Homo sapiens <400> 1028 ctgtagacat ttctttattg atatg 25 <210> 1029 <211> 25 <212> DNA <213> Homo sapiens <400> 1029 cagcaatttt ctaatatttg gttta 25 <210> 1030 <211> 25 <212> DNA <213> Homo sapiens <400> 1030 actcgaggtg tgcttaaggt tttct 25 <210> 1031 <211> 25 <212> DNA <213> Homo sapiens <400> 1031 gttttcttag agaacgttat tcgag 25 <210> 1032 <211> 25 <212> DNA <213> Homo sapiens <400> 1032 gtagtatatg ccctaaaacg tcagg 25 <210> 1033 <211> 25 <212> DNA <213> Homo sapiens <400> 1033 tatgccctaa aacgtcaggg gcgca 25 <210> 1034 <211> 25 <212> DNA <213> Homo sapiens <400> 1034 gccctaaaac gtcaggggcg cactc 25 <210> 1035 <211> 25 <212> DNA <213> Homo sapiens <400> 1035 tatggcttcg gcggctgaat ctaag 25 <210> 1036 <211> 25 <212> DNA <213> Homo sapiens <400> 1036 ttcggcggct gaatctaaga atacg 25 <210> 1037 <211> 25 <212> DNA <213> Homo sapiens <400> 1037 atctaagaat acgcggtctc ctgag 25 <210> 1038 <211> 25 <212> DNA <213> Homo sapiens <400> 1038 taagaatacg cggtctcctg agaac 25 <210> 1039 <211> 25 <212> DNA <213> Homo sapiens <400> 1039 gaatacgcgg tctcctgaga acttc 25 <210> 1040 <211> 25 <212> DNA <213> Homo sapiens <400> 1040 gcggtctcct gagaacttca aaaaa 25 <210> 1041 <211> 25 <212> DNA <213> Homo sapiens <400> 1041 cgctcacaaa gtcgtttaaa gagct 25 <210> 1042 <211> 25 <212> DNA <213> Homo sapiens <400> 1042 agctgaaatg cgttgcgaga atgag 25 <210> 1043 <211> 25 <212> DNA <213> Homo sapiens <400> 1043 tgaaatgcgt tgcgagaatg agttt 25 <210> 1044 <211> 25 <212> DNA <213> Homo sapiens <400> 1044 gtgacagcct gcataagaat gaatt 25 <210> 1045 <211> 25 <212> DNA <213> Homo sapiens <400> 1045 acagcctgca taagaatgaa ttgtg 25 <210> 1046 <211> 25 <212> DNA <213> Homo sapiens <400> 1046 atgaattgtg tttgccatga ccggc 25 <210> 1047 <211> 25 <212> DNA <213> Homo sapiens <400> 1047 tgtgtttgcc atgaccggcc acact 25 <210> 1048 <211> 25 <212> DNA <213> Homo sapiens <400> 1048 tgccatgacc ggccacactg tgaca 25 <210> 1049 <211> 25 <212> DNA <213> Homo sapiens <400> 1049 gaccggccac actgtgacaa aattt 25 <210> 1050 <211> 25 <212> DNA <213> Homo sapiens <400> 1050 acagtgccag ccaatgtgca gaggt 25 <210> 1051 <211> 25 <212> DNA <213> Homo sapiens <400> 1051 ccagccaatg tgcagaggtg gatga 25 <210> 1052 <211> 25 <212> DNA <213> Homo sapiens <400> 1052 cacggccctc aagctcctta agtga 25 <210> 1053 <211> 25 <212> DNA <213> Homo sapiens <400> 1053 cctcaagctc cttaagtgaa ttgcc 25 <210> 1054 <211> 25 <212> DNA <213> Homo sapiens <400> 1054 gtgctctttc atgcctatta tcagt 25 <210> 1055 <211> 25 <212> DNA <213> Homo sapiens <400> 1055 taggccagtt gtcaagtgtc aataa 25 <210> 1056 <211> 25 <212> DNA <213> Homo sapiens <400> 1056 tcaataaaag cgcatcatgt aattt 25 <210> 1057 <211> 25 <212> DNA <213> Homo sapiens <400> 1057 atgtgcccca tcgaaggcga aggga 25 <210> 1058 <211> 25 <212> DNA <213> Homo sapiens <400> 1058 aacattgcac gtttcttgtt ctctc 25 <210> 1059 <211> 25 <212> DNA <213> Homo sapiens <400> 1059 aagcataatg ctgtcaacgc aaccc 25 <210> 1060 <211> 25 <212> DNA <213> Homo sapiens <400> 1060 aatgctgtca acgcaaccct tatag 25 <210> 1061 <211> 25 <212> DNA <213> Homo sapiens <400> 1061 gcaaccctta tagatagctg ggtag 25 <210> 1062 <211> 25 <212> DNA <213> Homo sapiens <400> 1062 cttatagata gctgggtaga tattg 25 <210> 1063 <211> 25 <212> DNA <213> Homo sapiens <400> 1063 aaagccgctg ttttccgctc catga 25 <210> 1064 <211> 25 <212> DNA <213> Homo sapiens <400> 1064 agcccttggc tcgctgggaa tgaac 25 <210> 1065 <211> 25 <212> DNA <213> Homo sapiens <400> 1065 ctcaccgtag cagacgtggt gctgt 25 <210> 1066 <211> 25 <212> DNA <213> Homo sapiens <400> 1066 gtgctgtggt ctgtactcca gcaga 25 <210> 1067 <211> 25 <212> DNA <213> Homo sapiens <400> 1067 tggtctgtac tccagcagat cggag 25 <210> 1068 <211> 25 <212> DNA <213> Homo sapiens <400> 1068 gtactccagc agatcggagg ctgca 25 <210> 1069 <211> 25 <212> DNA <213> Homo sapiens <400> 1069 agtgtgacag tgccagccaa tgtgc 25

Claims (6)

  1. (ⅰ) 대장암 조직에서 특이적으로 발현되는 유전자와 결합할 수 있는 유전자 탐침;
    (ⅱ) 올리고(dT)15, -(CH2)6- 및 아민(amine)기를 순차적으로 포함하고, 전기 유전자 탐침의 5' 말단이 올리고(dT)15의 3'말단부위에 연결된 링커; 및,
    (ⅲ) 표면에 알데히드가 결합되고, 전기 링커의 아민기가 표면의 알데히드기와 시프염기(Schiff's base)반응을 통해 연결된 고체를 포함하는, 대장암 진단용 DNA 칩.
  2. 제 1항에 있어서,
    유전자 탐침은 서열번호 1 내지 1069의 염기서열로 표시되는 올리고뉴클레오티드로부터 선택된 1개 이상의 올리고뉴클레오티드인 것을 특징으로 하는
    대장암 진단용 DNA 칩.
  3. (ⅰ) 대장암 조직에서 특이적으로 발현되는 유전자와 결합할 수 있는 서열번호 1 내지 20, 21 내지 40, 41 내지 60, 61 내지 80, 101 내지 120, 121 내지 140, 241 내지 260, 321 내지 340 및 361 내지 380의 유전자 탐침;
    (ⅱ) 올리고(dT)15, -(CH2)6- 및 아민(amine)기를 순차적으로 포함하고, 전기 유전자 탐침의 5' 말단이 올리고(dT)15의 3'말단부위에 연결된 링커; 및,
    (ⅲ) 표면에 알데히드가 결합되고, 전기 링커의 아민기가 표면의 알데히드기와 시프염기(Schiff's base) 반응을 통해 연결된 고체를 포함하는, 대장암 진단용 DNA 칩.
  4. 제 1항의 대장암 진단용 DNA 칩 및 시료에서 채취한 RNA로부터 합성된 cDNA를 표지하기 위하여 형광표지된 데옥시 뉴클레오타이드를 포함하는, 대장암 진단용 키트.
  5. 제 4항에 있어서,
    형광표지된 데옥시 뉴클레오타이드는 텍사스 레드(Texas Red) dATP, 시아닌 3(cyanine 3) dCTP, 시아닌 5(cyanine 5) dGTP 또는 플루오레세인-12(fluorescein-12) dUTP인 것을 특징으로 하는
    대장암 진단용 키트.
  6. 제 3항의 대장암 진단용 DNA 칩 및 시료에서 채취한 RNA로부터 합성된 cDNA를 표지하기 위하여 형광표지된 데옥시 뉴클레오타이드를 포함하는, 대장암 진단용 키트.
KR1020040039303A 2004-05-31 2004-05-31 대장암 진단용 ｄｎａ 칩 KR20050114099A (ko)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101104105B1 (ko) * 2007-12-14 2012-01-12 한국생명공학연구원 대장암 진단용 조성물 및 그 용도
KR101232130B1 (ko) * 2008-12-16 2013-02-12 연세대학교 산학협력단 인간의 장암 진단용 키트
KR101416504B1 (ko) * 2011-03-31 2014-08-06 연세대학교 산학협력단 인간의 장암 진단용 키트
KR101416502B1 (ko) * 2011-03-31 2014-08-06 연세대학교 산학협력단 인간의 장암 진단용 키트
KR101416503B1 (ko) * 2011-03-31 2014-08-06 연세대학교 산학협력단 인간의 장암 진단용 키트

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101104105B1 (ko) * 2007-12-14 2012-01-12 한국생명공학연구원 대장암 진단용 조성물 및 그 용도
KR101232130B1 (ko) * 2008-12-16 2013-02-12 연세대학교 산학협력단 인간의 장암 진단용 키트
KR101416504B1 (ko) * 2011-03-31 2014-08-06 연세대학교 산학협력단 인간의 장암 진단용 키트
KR101416502B1 (ko) * 2011-03-31 2014-08-06 연세대학교 산학협력단 인간의 장암 진단용 키트
KR101416503B1 (ko) * 2011-03-31 2014-08-06 연세대학교 산학협력단 인간의 장암 진단용 키트

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