KR20050102954A - A composition for probiotic functions comprising mixed live microorganims, yeast cultured composition and mixed enzymes as active ingredients - Google Patents

Abstract

The present invention relates to a physiologically active composition comprising an animal feed additive complex probiotic, a yeast culture and a complex enzyme as an active ingredient, which is added to an animal feed to improve feed intake, increase weight gain and feed utilization of livestock, and intestinal bacteria You can expect a formal effect and diarrhea prevention effect according to the change of.

Description

A composition for probiotic functions comprising mixed live microorganims, yeast cultured composition and mixed enzymes as active ingredients

The present invention relates to a composition for promoting livestock growth containing a complex probiotic, yeast culture, and a complex enzyme for feed additives as an active ingredient. The composition of the present invention is a composition having the effects of improving the palatability and feed intake, increase the daily weight gain, improve feed utilization and the probiotics effect added to the animal feed.

For a long time, the livestock industry has been supplying various additives to feed for the purpose of preventing livestock diseases and increasing productivity. However, many kinds of artificially prepared chemicals, including antibiotics and antibacterial agents, have appeared to be resistant to many pathogenic microorganisms due to their continued use, and they remain in meat and milk, causing adverse effects on the human body. This occurred. The growing public's concern about health and the recognition of these side effects have led to widespread perception of antibiotics and chemically processed feeds and livestock products. In Europe, antibiotics used in humans are not allowed to be used as animal growth promoters. I came to a state. Recently, in addition to consumer demand, the emergence of resistant drugs due to human drugs and cross-resistance, has led to strict regulations on the types and residues of antibiotics available internationally. At present, this trend continues to spread throughout the world, and in the future, the use of antibiotics or antimicrobials for growth promoters will be more stringent. Accordingly, the replacement additives that supplement the shortcomings of antibiotics have recently been attracting attention and are rapidly being replaced by eco-friendly products. Representatives include natural additives such as probiotics, enzymes, yeast cultures, and certain immunoactive substances extracted from them (specific oligosaccharides, met oligosaccharides, glucans, etc.). I am getting it.

The term probiotic was first used by Lilly and Stillwell (Lilly D. M. et al., Science, 'Probiotics: growth promoting factors produced by microorganism', 147; 747, 1996) and refers to living microbial agents. Parker (Parker R. B., An Nutr & Health, 29; 4-8, 1974) defined probiotics as 'living microorganisms that affect intestinal microbial balance'.

   Probiotics allow the intestinal flora to be well developed, leading to the maturation of beneficial microbial communities in the gut with the concept of competitive exclusion, called the 'Nurmi concept', which can subsequently prevent the proximity of pathogenic microorganisms such as Salmonella (Nurmi E. et al. , Nature, 241; 210, 1973), produce vitamins, enzymes and nutrients in the intestine, and break down nutrients that the host has not digested and feed them to the host to improve livestock growth and feed demand.

  In fact, Tortuero reported that cultivation of Lactobacillus acidophilus in broilers affects the flora of the caecum and colon (Tortuero F., Poultry Sci. 52; 197-203, 1973), and Han et al. It was reported that the number of harmful bacteria such as coliforms was decreased (In-Kyu et al., Korean Journal of Animal Science, 26 (2); 150-157, 1984). Kim et al. Reported that the use of complex probiotics such as Bacillus subtilis and Aspergillus oryzae decreased the number of harmful bacteria, such as coliforms, in fungal corn (Kim Chang-jong et al., 30 (9); 542-548, 1988). Probiotics have also been reported to be used to treat abnormal gut flora caused by diseases such as acute rotavirus diarrhea, food allergy and colon cancer (Salminen S. et al., Antonie). Van Leeuwenhoek, 70 (2-4); 347-358, 1996).

  The use of probiotics in broilers was first initiated by Tortuero (1973). According to this study, Lactobacillus acidophilus was cultured and fed to broiler chickens. Subsequently, it was reported that livestock growth was induced through experiments in which Lactobacillus spp. Was cultured and fed to broilers (Burkett 1977 Dilworth 1978 Watkins 1996 Mohan 1996 Yeo 1997 Jin 1998).

  Aspergillus oryzae is an aerobic fungus with a very wide growth pH and temperature range and can use various organic waste resources as substrates (Garraway, Evans, 1984). The enzymes produced are amylase, xylanase and protease. Increasing the feed value (Petterson et al., 1989) and supplementing the action of heat-resistant enzymes produced in the animal body to facilitate the digestion and absorption of nutrients (Graham et al., 1988). In addition to synthesizing various vitamins such as riboflavin, folic acid and nicotinic acid to about 60%, it is known to promote livestock growth as an unknown growth factor (UGF) source produced during fermentation (Russo and Lfeimann, 1959; Tsang and Schaible 1960)

Feeding of fungi and yeast cultures was associated with changes in the rumen microbial community (Wiedmeier et al. 1987; Frumholtz et al., 1989; Fondevila et al., 1990), VFA production and its proportions (Wiedmeier et al., 1987) Kellems et al., 1990), and improved digestibility and feed utilization (Wiedmeier et al. 1987; Fondevila et al., 1990) have been reported to improve livestock productivity by improving production and weight gain (Kellems et al. 1990).

  However, there are some difficulties in the use of probiotics as feed additives. For example, when Lactobacillus is fed to young piglets for use as feed additives, it has been reported that the improvement of weight gain was shown only in the treatment containing lactose. (Pollman 1980), Lactobacillus strains also have limitations in the resistance to intestinal enzymes and acid resistance to gastric acid, which can of course be solved by physical processing, but not so desirable considering economics. have. In addition, there are limitations in the heat resistance required for the production of processed feed. To overcome this limitation, spore forming bacteria such as B. subtilis or B. coagulans (L. sporegenes) have been used.

There was a report that feeding B. coagulans (L. sporegenes) to broilers improved the weight gain and reduced the number of harmful bacteria such as coliforms (Han et al., 1984). There have been reports of decreased cholesterol content in liver and serum (Santoso, 1995). In addition, rats were fed B. subtilis to affect Lactobacillus spp. In the rats. L. murinus and B. subtilis were cultured together in vitro to increase the growth of L. murinus. Subtilis has been suggested to affect the growth of lactic acid bacteria (Hosoi, 1999).

However, the endogenous spore forming bacteria also have a number of limitations, one of which is a limitation in how many endogenous spore forming bacteria form resistant spores due to problems in the manufacturing process during probiotic production, and the second is formed spores. This is a limitation in how quickly the spores can be removed and attached to the intestine after reaching the small intestine after passing through scissors.

The present inventors overcome the limitations of the conventional probiotics as described above, supply nutrients that are easily lacking, improve feed utilization and stabilize the intestinal bacterial flora so as to maximize the original advantages and bring about a synergy effect (synergy effect). In order to prevent diseases caused by pathogenic microorganisms, and to increase the immunity of livestock by secreting physiologically active substances, a composition containing an effective complex probiotic, yeast culture and complex enzyme as an active ingredient was developed.

The purpose of the present invention is to maximize the advantages of each probiotic, yeast, enzyme and supplement the shortcomings to supply nutrients, such as unknown growth factors, insufficient nutrients, improve feed availability and disease caused by pathogenic microorganisms through stabilization of the intestinal flora The purpose of the present invention is to provide a composite probiotic, a yeast culture, and a composition containing a complex enzyme as an active ingredient, which are useful as an effective feed additive for preventing and adding immunity due to the added bioactive substance and the bioactive substance secreted by live bacteria. have.

In order to achieve the object of the present invention, 10 to 30% (by weight) of complex probiotic, 5 to 20% (by weight) of yeast culture, and 0.1 to 1.0% (by weight) of complex enzyme (also called “Enzyme cocktail”) are mixed. To prepare a composition for promoting livestock growth.

The following describes the present invention specifically. (Hereinafter, percentage means weight%.)

Fermentation medium of 1) complex biocide added to the present invention is about 10-30% of herbal medicines, about 10-15% of seaweeds and aquatic by-products, about 5-10% of wild vegetables, about 2-5% of crab shells, about 2-5 of fishmeal %, Rice bran about 10-25%, ink about 3-7%, grain skin about 13-17%, minerals selected from vermiculite, zeolite and elvan, about 5-9%, ocher about 1-3%. Bacillus subtilis, Bacillus thuringiensis, lactic acid bacteria, photosynthetic bacteria (Cyanobacteria, etc.), yeast, gram-positive actinomycetes (Streptomyces spp.) In the medium And aerobic fermentation, anaerobic fermentation, and ripening fermentation using complex microorganisms such as fungi (Aspergillus oryzae, Aspergillus spp., Penicillium, etc.). The total number of strains contained in the composition was 2 photosynthetic bacteria (4 × 10 ⁵ ), 6 yeasts ( 6.2 × 10 ⁶ ), three fungi (1.1 × 10 ⁶ ), four proteolytic bacteria (2 × 10 ⁴ ), and lipolytic bacteria (6.5 × 10 ⁶ ).

   Herbal medicines used in the present invention can be used one or two or more of the herbs selected from Angelica, Licorice, Cheongung, Peony, Sinchocho, Astragalus.

  As the algae and marine by-products used in the present invention, one or two or more components selected from Locust larvae, Haitai, seaweed and kelp can be used.

   As the wild grass that can be used in the present invention, one or two or more kinds of wild grass selected from Myungju, Mosquito, Saejanggye, bellflower, and round can be used.

   As the grain hides used in the present invention, one or more selected from soybean seed, cotton thread, and oxpi may be used.

   Cultivation: 1) about 10-30% of herbal medicines, about 10-15% of seaweeds and marine by-products, about 5-10% of wild grasses, about 2-5% of crab shells, about 2-5% of fishmeal, about 10-25% of rice bran, Put fermentation broth mixed with about 3-7% ink, about 13-17% grain powder, about 5-9% minerals selected from vermiculite, zeolite and elvan, and about 1-3% ocher. After

   2) after aerobic fermentation at low, medium and high temperature for 10 to 12 hours,

   3) The obtained fermented product is transferred to an anaerobic adiabatic fermentation vessel and fermented in anaerobic state,

   4) The fermentation product is transferred to a liquid bottom fermenter to produce a composite probiotic through a fermentation process of 20 to 30 days at low, medium and high temperatures.

   The aerobic fermentation of the second step is to proceed with fermentation gradually increasing the temperature by heating while injecting air, starting at room temperature, low temperature fermentation is to ferment for 2 to 3 hours at a temperature from room temperature to 50 ℃, medium temperature Fermentation is a fermentation for 2 to 3 hours at a temperature of 50 to 70 ℃, high temperature fermentation is fermented for 1 to 2 hours at a temperature of 70 to 80 ℃.

   In the anaerobic fermentation of step 3) it is fermented for 6 to 8 days under the condition of 35 to 45 ℃.

   The fermentation fermentation of step 4) is anaerobic fermentation for 20 to 30 days under the condition of pH 3.3 ± 0.2 under the condition that the temperature of 95 ± 3 ℃ by pure fermentation heat is maintained.

   The fermentation product of 4) is solid-liquid separated for 5 to 7 days by a natural dehydration method in a dehydrator, and the liquid is stored in a stable tank and can be used as an additive for plant cultivation fertilizer or nutrient solution, and the separated solid is used for animal breeding. Used as an additive.

   The complex probiotic used in the present invention utilizes this solid. (This combination probiotic is pending patent application under patent application number 2000-44427.)

2) Yeast culture used in the present invention (Yeast culture) is isolated saccharomyces cerevisiae (Saccharomyces cerevisiae) corn syrup, hydrolyzed corn flour, yeast extract, casein, trace minerals, vitamins Bacillus subtilis (Bacillus subtilis) cultured in a fermentation medium consisting of vitamin premix including star B, starch, mono potassium phosphate, potassium di-phosphate and potassium maltose subtilis) and Bacillus licheniformis cells and cultures are added. It contains Saccharomyces cerevisiae 5.0 × 10 ¹² CFU / kg and Bacillus subtilis and Bacillus licheniformis 1.5 × 10 ¹⁰ CFU / kg.

  This yeast extract is commercially available and can be purchased and used, or can be cultured and used.

  3) Enzyme cocktail used in the present invention contains more than 22,000 FIP amylase, 1,600 FIPase, lipase 28,000 FIP per g. Amylases, proteases and lipases used in the present invention are commercially available and use commercially available enzymes.

   The composition of the present invention uses 0.05 to 0.5% by weight, preferably 0.1 to 0.3% by weight relative to the basic feed for animal breeding.

  Next, the present invention will be described in more detail as examples and test examples.

Example 1

Preparation of complex probiotic, yeast and complex enzyme composition for growth promotion

   Commercially available yeast culture 5-20%, complex probiotics (patent pending 2000-44427, pending patent application, purchased from the applicant) 10-30% mixed, Enzyme cocktail 0.1 ~ 1.0% It was prepared by mixing.

Experimental Example 1

Effect on weight gain in broilers

0.1% of the composition of the present invention is added to broiler broiler feed, followed by 40 days of feeding, and the average weight gain (g), the average feed intake (g), the feed requirement and the number of enteric coliforms ( CFU) was compared with the control group fed the feed without addition of the composition of the present invention. The results were as shown in Table 1 below.

Table 1: Effect on weight gain in broilers

As confirmed in Table 1, the composition of the present invention showed an excellent effect on the average weight gain (g), average feed intake (g), feed requirement and enteric coliform count (CFU) compared to the non-added control group.

Experimental Example 2

Effect of Weaning Pig Growth and Mortality

Weaned piglets were divided into 0.1% of the composition of the present invention, 0.1% added to the head culture, and non-administered controls, and fed for 63 days and fed with the starting weight (kg) and the end weight as in the following normal feed. (kg) Weight gain per day (g), feed intake per day (g) and feed demand were measured respectively. The results are shown in Table 2 below.

Table 2: Effect on weight gain and mortality of weaning piglets

As confirmed in Table 2, the composition of the present invention showed an excellent effect on the end weight (kg), daily gain, daily feed intake and feed rate compared to the non-added control and yeast culture administration group.

As described above, the composition of the present invention can be added to feed to feed livestock to increase feed intake of livestock, improve feed availability, and stabilize the intestinal bacterial flora and disease suppression due to various physiologically active substances.

Claims (2)

Livestock growth promoting composition comprising a probiotic complex probiotic, yeast culture and complex enzyme as an active ingredient. The composition for promoting animal growth according to claim 1, comprising 10 to 30% by weight of complex probiotic, 5 to 20% by weight of yeast culture, and 0.1 to 1.0% by weight of complex enzyme.