KR20050092318A - Skin whitening cosmetic containing a herb extract with inhibitory activity of melanin formation - Google Patents

Abstract

The present invention relates to a skin whitening cosmetic containing a plant extract having a melanin production inhibitory activity, the skin whitening cosmetics according to the present invention is effective in any one selected from the group consisting of small locust extract, ligature extract and mixtures thereof It is characterized by containing as a component. Soybean ingot extract and alliance extract are natural substances that can be used safely without any side effects on the skin. They also inhibit melanin production and are excellent in inhibiting pigmentation. Therefore, cosmetics containing them as active ingredients are useful for skin whitening such as blemishes and freckles. Very effective.

Description

Skin whitening cosmetic containing a herb extract with inhibitory activity of melanin formation}

The present invention relates to a skin whitening cosmetic, and more particularly to a skin whitening cosmetic containing a plant extract having a melanin production inhibitory activity can be used safely without side effects on the skin.

It is everyone's constant desire to have white, fair skin. Human skin color is determined by the concentration and distribution of melanin in the skin. In addition to genetic factors, it is also influenced by environmental or physiological conditions such as ultraviolet rays, fatigue and stress. Melanin is a type of amino acid tyrosine (tyrosinase) acts as an enzyme called tyrosinase (tyrosinase) is converted to dopa (DOPA), dopaquinone (dopaquinone) is produced through a non-enzymatic oxidation reaction. However, although the pathway by which melanin is made is known, it is still not clear what mechanisms induce melanin synthesis, the previous step in which tyrosinase works.

When such synthesis of melanin occurs excessively in the skin, it may darken the skin tone, and may cause spots and freckles. Therefore, by inhibiting the synthesis of melanin pigment in the skin, not only can brighten the skin tone to realize skin whitening, but also improve skin hyperpigmentation such as spots, freckles, etc. caused by ultraviolet rays, hormones and genetic causes. have.

Therefore, conventionally, a substance having an inhibitory activity against tyrosinase, such as hydroquinone, ascorbic acid, kojic acid, glutathione, and the like, is formulated into a cosmetic such as an ointment or an essence. Whitening was realized or skin hyperpigmentation such as blemishes and freckles was improved. However, hydroquinone exhibits a predetermined whitening effect, but has a problem in that skin irritation is severe and the amount of the compound is limited to a very small amount, and ascorbic acid is easily oxidized, resulting in problems such as discoloration and discoloration. There is this. In addition, thiol-based compounds such as glutathione and cysteine not only have a characteristic unpleasant odor, but also have problems with transdermal absorption, and glycosides and derivatives thereof have high polarity, making it difficult to use as a cosmetic ingredient. Placenta extract, on the other hand, has no irritation to the skin, but lacks a whitening effect.

Therefore, the technical problem to be achieved by the present invention is to solve the above problems can be used safely without side effects on the skin, and to provide a cosmetic for skin whitening excellent in inhibiting the pigmentation effect by inhibiting the production of melanin.

In order to achieve the above technical problem, the present invention provides a cosmetic for skin whitening containing any one selected from the group consisting of small sorbent extract, ligature extract and mixtures thereof as an active ingredient.

Preferably, the dry weight of any one selected from the group consisting of the oleophilic extract, the alliance extract, and a mixture thereof is 0.0001 to 10.0 wt% based on the total weight of the cosmetic.

In addition, the pulverulent lump extract or ligature extract is preferably extracted with one or more solvents selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, ethyl acetate, and acetone. More preferably, the extracted extract is distilled under reduced pressure and lyophilized or spray dried.

Hereinafter, the cosmetic for skin whitening according to the present invention will be described in detail.

Cosmetics for skin whitening according to the present invention comprises a sorbate extract, ligature extract, or a mixture thereof, which is a natural substance having melanin production inhibitory activity, as an active ingredient.

Minor masses use the roots of Jasminum floridum Bge., A plant of the family Asteraceae, and have been used to heal wounds.

The coalition is an outpost of the legume Aeschynomene indica L., which is used to treat colds, jaundice, dermatitis, and eczema.

The present inventors have conducted experiments on mouse melanoma cells (B16 mouse melanoma cells) that can screen to substances that inhibit melanin synthesis induction by using extracts of leucine and coalescence, and they are very strong melanin. It was found to exhibit a production inhibitory effect and a whitening effect. Therefore, the addition of extracts of leucine and / or ligatures, which are natural substances, to cosmetics such as skin ointments, essences, creams, etc., may exhibit a powerful skin whitening effect without any side effects.

Glucose or coalescence can be easily purchased on the market, and their extracts are, for example, after heating and extracting the oil rush or coalescence with an organic solvent such as water or anhydrous or hydrous lower alcohol, ethyl acetate, acetone having 1 to 4 carbon atoms. After concentration under reduced pressure, it can be obtained by lyophilization or spray drying. To obtain a high purity extract, the extract may be extracted one or more times in the same manner.

Lactobacillus or pulmonary extract obtained as described above may be used in each or at the same time contained in various cosmetics such as skin ointment or lotion. The content of the pulmonary ingot extract, ligature extract or mixture thereof contained in the preparation of the cosmetic according to the present invention preferably has a dry weight of 0.0001 to 10.0% by weight based on the total weight of the cosmetic, more preferably 0.0005 to 10.0% by weight. %to be. If the content is less than 0.0001% by weight, it is difficult to expect a clear melanin production inhibitory effect and a whitening effect, and when it exceeds 10.0% by weight, no clear effect is expected as the increasing content.

Hereinafter, the present invention will be described in detail with reference to Examples. However, embodiments according to the present invention can be modified in many different forms, the scope of the present invention should not be construed as limited to the embodiments described below. Embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

Example of pulverization and coalescence extraction

Extraction Example 1

The commercially available pulverized pulverized crushed 100g was added to 3L of 80% methanol, boiled for 3 hours in a reflux extractor with a cooling capacitor and extracted. Subsequently, the resultant was filtered through a 100 mesh filter cloth, and the residue was extracted once more in the same manner. Each extract was combined and filtered using Whatman No. 2 filter paper at room temperature to remove insoluble materials, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser, and lyophilized to obtain 6.5 g of dry extract.

Extraction Example 2-6

Except for changing the solvent according to the description in Table 1 instead of 80% methanol was carried out in the same manner as in Extraction Example 1.

Extraction Example 7

The same procedure as in Extraction Example 1 was conducted except that coalescence was used instead of small lumps.

Extraction Example 8-12

Except for changing the solvent according to the description in Table 1 instead of 80% methanol was carried out in the same manner as in Extraction Example 7.

sample menstruum Dry extract (g) Extraction Example 1 Small mass 80% methanol 5.5 Extraction Example 2 Small mass Purified water 5.1 Extraction Example 3 Small mass 85% ethanol 5.6 Extraction Example 4 Small mass 70% acetone 5.2 Extraction Example 5 Small mass Ethyl acetate 3.7 Extraction Example 6 Small mass chloroform 3.3 Extraction Example 7 Alliance 80% methanol 6.3 Extraction Example 8 Alliance Purified water 6.4 Extraction Example 9 Alliance 85% ethanol 6.6 Extraction Example 10 Alliance 70% acetone 5.8 Extraction Example 11 Alliance Ethyl acetate 4.4 Extraction Example 12 Alliance chloroform 3.5

Examples 1-12

Each of the extracts obtained in accordance with the extraction examples 1 to 12 and the aqueous hydroquinone solution was added to the culture of mouse melanoma cells (B-16 mouse melanoma cells) to test the whitening effect at the cellular level (Lotan R., Lotan D. Cancer Res. 40: 3345-3350, 1980). Each of the extracts obtained in accordance with the extraction examples 1 to 12 to a final concentration of 0.005% (Examples 1 to 12), the hydroquinone aqueous solution was added to the culture medium of B-16 melanoma cells to a concentration of 0.0001% After incubation for 3 days, the cells were trypsin-treated and removed from the culture vessel and centrifuged to extract melanin.

1 ml of sodium hydroxide solution (1N concentration) was added thereto to boil for 10 minutes to dissolve the melanin, and the absorbance was measured at 400 nm using a spectrophotometer to indicate the amount of melanin produced per unit cell count (10 ⁶ cell). In addition, the relative melanin production relative to the control was calculated as the inhibition rate (%) and the results are shown in Table 2 below.

sample Melanin production  % Inhibition Control group (no addition) 0.048 ± 0.003 - Example 1 (final concentration 0.005%) 0.028 ± 0.003 41% Example 2 (final concentration 0.005%) 0.035 ± 0.002 28% Example 3 (final concentration 0.005%) 0.027 ± 0.002 43% Example 4 (final concentration 0.005%) 0.027 ± 0.002 44% Example 5 (final concentration 0.005%) 0.030 ± 0.003 37% Example 6 (final concentration 0.005%) 0.032 ± 0.003 33% Example 7 (final concentration 0.005%) 0.033 ± 0.004 32% Example 8 (final concentration 0.005%) 0.034 ± 0.002 29% Example 9 (final concentration 0.005%) 0.035 ± 0.002 28% Example 10 (final concentration 0.005%) 0.030 ± 0.002 38% Example 11 (final concentration 0.005%) 0.027 ± 0.002 43% Example 12 (final concentration 0.005%) 0.034 ± 0.003 30% Hydroquinone (final concentration 0.0001%) 0.025 ± 0.002 48%

                                             * Repeat count = 3

Referring to Table 2, it can be seen that each sample obtained in accordance with the extraction examples 1 to 12 has a very strong melanin production inhibitory ability against the melanoma cells of the cultured mice compared to the control group (Examples 1 to 12) Considering that it is an extract that is not purified at all, it can be seen that the effect is not significantly reduced when compared with hydroquinone, which is a previously known whitening substance.

Examples 13-20

The melanoma production inhibitory ability of the rat melanoma cells cultured in the same manner as in Example 1 according to the concentration change of the extracts of the extraction examples 1 and 7 was investigated, and the results are shown in Table 3 below.

sample Application concentration (%) Melanin production % Inhibition Control group (no addition) - 0.048 ± 0.004 - Example 13 Extract of Extraction Example 1 Final concentration (0.0005%) 0.037 ± 0.003 23% Example 14 Final concentration (0.005%) 0.030 ± 0.003 37% Example 15 Final concentration (0.05%) 0.028 ± 0.002 43% Example 16 Final concentration (0.5%) 0.025 ± 0.001 48% Example 17 Extract of Extraction Example 7 Final concentration (0.0005%) 0.036 ± 0.002 26% Example 18 Final concentration (0.005%) 0.033 ± 0.004 32% Example 19 Final concentration (0.05%) 0.029 ± 0.002 39% Example 20 Final concentration (0.5%) 0.026 ± 0.002 45% Hydroquinone Final concentration (0.0001%) 0.025 ± 0.002 48%

                                                   * Repeat count = 3

As shown in the results of Table 3, it can be seen that the higher the concentration of the leucine mass and the alliance extract, the greater the inhibition of melanin production against the cultured melanoma cells.

On the other hand, hydroquinone has a strong melanin production inhibitory ability, but exhibits severe cytotoxicity even at low concentration of 0.0001%, natural plant extracts according to the present invention does not show cytotoxicity even at 0.05% or more concentration, inhibiting melanin production higher than hydroquinone You can have an effect.

In the following, the sorbate or ligature extract is added to the external skin ointment, cream, soft cosmetics, nutrient cosmetics, packs, essences to prepare a cosmetic, and look at the pigmentation inhibitory effect appeared by prescribing them to the test subjects.

Preparation Examples 1 and 2

The leucine ingot extract obtained from Extraction Example 5 and the allied extract obtained from Extraction Example 11 were each added as an active ingredient, and a skin external ointment was prepared using the ingredients and contents shown in Table 4 below.

Comparative Example 1

Without adding a plant extract, the skin external ointment was prepared with the ingredients and contents shown in Table 4 below.

Composition (% by weight) Production Example Comparative example One 2 One Plant extracts Hypothelium Extract 10 - - Mingled extract - 10 - Diethyl sebacate 8 8 8 Prepayment 5 5 5 Polyoxyethylene oleyl ether phosphate 6 6 6 Sodium benzoate Quantity Quantity Quantity vaseline to 100 to 100 to 100

Preparation Examples 3 and 4

The leucine lump extract obtained from the extraction example 5 and the ligature extract obtained from the extraction example 11 were each added as an active ingredient, and a cream was prepared according to the ingredients and contents shown in Table 5 below.

Comparative Example 2

Cream was prepared with the ingredients and contents shown in Table 5 below without the addition of plant extracts.

Composition (% by weight) Production Example Comparative example 3 4 2 Plant extracts Hypothelium Extract 1.0 - - Mingled extract - 1.0 - Stearic acid 15.0 15.0 15.0 Cetanol 1.0 1.0 1.0 Potassium hydroxide 0.7 0.7 0.7 glycerin 5.0 5.0 5.0 Propylene glycol 3.0 3.0 3.0 antiseptic Quantity Quantity Quantity incense Quantity Quantity Quantity Purified water to 100 to 100 to 100

Preparation Examples 5 and 6

Small lump extract obtained from the extraction example 5 and the alliance extract obtained from the extraction example 11 were added as an active ingredient, respectively, and the softening soft water was prepared with the components and contents shown in Table 6 below.

Comparative Example 3

Without adding a plant extract, softening water was prepared with the ingredients and contents shown in Table 6.

Composition (% by weight) Production Example Comparative example 5 6 3 Plant extracts Hypothelium Extract 0.2 - - Mingled extract - 0.2 - ethanol 10.0 10.0 10.0 Polylauric acid polyoxyethylene sorbitan 1.0 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 0.2 glycerin 5.0 5.0 5.0 1,3-butylene glycol 6.0 6.0 6.0 incense Quantity Quantity Quantity Pigment Quantity Quantity Quantity Purified water to 100 to 100 to 100

Preparation Examples 7 and 8

The leucine lump extract obtained from the extraction example 5 and the alliance extract obtained from the extraction example 11 were added as an active ingredient, and nutrient cosmetic water was prepared according to the ingredients and contents shown in Table 7 below.

Comparative Example 4

No nutrient extract was prepared using the ingredients and contents shown in Table 7 below without adding a plant extract.

Composition (% by weight) Production Example Comparative example 7 8 4 Plant extracts Hypothelium Extract 0.1 - - Mingled extract - 0.1 - Waselin 2.0 2.0 2.0 Sesquioleate sorbitan 0.8 0.8 0.8 Polyoxyethylene oleylethyl 1.2 1.2 1.2 Methyl paraoxybenzoate Quantity Quantity Quantity Propylene glycol 5.0 5.0 5.0 ethanol 3.2 3.2 3.2 Carboxy Vinyl Polymer 18.0 18.0 18.0 Potassium hydroxide 0.1 0.1 0.1 Pigment Quantity Quantity Quantity incense Quantity Quantity Quantity Purified water to 100 to 100 to 100

Preparation Examples 9 and 10

The leucine lump extract obtained from the extraction example 5 and the ligature extract obtained from the extraction example 11 were each added as an active ingredient, and a pack was prepared with the ingredients and contents shown in Table 8 below.

Comparative Example 5

Packs were prepared with the ingredients and contents shown in Table 8 below without the addition of plant extracts.

Composition (% by weight) Production Example Comparative example 9 10 5 Plant extracts Hypothelium Extract 0.5 - - Mingled extract - 0.5 - glycerin 5.0 5.0 5.0 Propylene glycol 4.0 4.0 4.0 Polyvinyl alcohol 15.0 15.0 15.0 ethanol 8.0 8.0 8.0 Polyoxyethylene oleylethyl 1.0 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 0.2 incense Quantity Quantity Quantity Pigment Quantity Quantity Quantity Purified water to 100 to 100 to 100

Preparation Examples 11 and 12

The leucine lump extract obtained from the extraction example 5 and the ligature extract obtained from the extraction example 11 were each added as an active ingredient, and an essence was prepared according to the ingredients and contents shown in Table 9 below.

Comparative Example 6

Essence was prepared with the ingredients and contents shown in Table 9 below without the addition of plant extracts.

Composition (% by weight) Production Example Comparative example 11 12 6 Plant extracts Hypothelium Extract 5 - - Mingled extract - 5 - Propylene glycol 10.0 10.0 10.0 glycerin 10.0 10.0 10.0 Sodium hyaluronate aqueous solution (1%) 5.0 5.0 5.0 ethanol 5.0 5.0 5.0 Polyoxyethylene Cured Castor Oil 1.0 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 0.1 incense Quantity Quantity Quantity Purified water to 100 to 100 to 100

The method used to verify the pigmentation inhibitory effect by the skin external ointment, cream, supple cosmetics, nutrient cosmetics, pack, essence prepared as described above is as follows.

First, select 20 healthy men and women and attach aluminum foil with two rows of 6 holes with a diameter of 7 mm in the lower part of both arms. Was investigated. The irradiation site was washed well with 70% ethanol aqueous solution before irradiation. Twice a day from 3 days before irradiation to 3 weeks after irradiation, containing cosmetics containing leucine or pulmonary extracts prepared according to Preparation Examples 1 to 12, and bases containing no plant extracts according to Comparative Examples 1 to 6. It was applied in the same row in pairs. Here, the pack agents of Production Examples 9, 10 and Comparative Example 5 were removed 15 minutes after the application.

After prescribing the cosmetics according to the respective production examples and comparative examples according to the above method, the degree of pigmentation was visually determined, and the effect of the pigmentation inhibition of the cosmetics according to each example and the cosmetics according to the comparative example was compared to the apparent effect. Evaluated in three stages, there is no effect, no difference, and also examined the occurrence of skin side effects are shown in Table 10 below.

Experimental substance Distinct Effects (persons) Effective (persons) No difference (persons)  Side effects (persons) Preparation Example 1 3 9 8 0 Preparation Example 2 4 9 7 0 Preparation Example 3 One 9 10 0 Preparation Example 4 One 7 12 0 Preparation Example 5 2 6 10 0 Preparation Example 6 One 8 12 0 Preparation Example 7 0 8 11 0 Preparation Example 8 2 6 12 0 Preparation Example 9 2 6 12 0 Preparation Example 10 3 6 12 0 Preparation Example 11 3 8 9 0 Preparation Example 12 3 7 10 0

As shown in Table 10, at least 8 or more of 20 cosmetic subjects containing the leucine ingot or pulmonary extract prepared according to Preparation Examples 1 to 12 exhibited a skin whitening effect, in particular, a large amount of leucine ingot, The cosmetics of Preparation Examples 1 and 2 containing the Pleurotus extract exhibited a clear pigmentation inhibitory effect of at least 60% and 65%, respectively, than the cosmetics of Comparative Example 1 containing no plant extract, and did not show any side effects in the skin. Able to know.

As described above, the cosmetic for skin whitening containing the oleander or pulmonary extract of the present invention inhibits melanin production and inhibits pigmentation, and thus is effective in improving skin whitening or freckles. In addition, because the sorbate and coalescence extract is a natural substance can be used safely as a cosmetic without side effects.

Claims (3)

Skin whitening cosmetics, characterized in that it contains any one selected from the group consisting of leucine lump extract, ligature extract and mixtures thereof. The cosmetic composition for skin whitening according to claim 1, wherein the dry weight of any one selected from the group consisting of the oleophilic extract, the allied extract, and a mixture thereof is 0.0001 to 10.0 wt% based on the total weight of the cosmetic. The skin whitening cosmetic according to claim 1, wherein the pulverulent lump extract or pulmonary extract is extracted with one or more solvents selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, ethyl acetate, and acetone.