KR20050055660A - Manufacturing method of a microorganism culture solution - Google Patents
Manufacturing method of a microorganism culture solution Download PDFInfo
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- KR20050055660A KR20050055660A KR1020050041399A KR20050041399A KR20050055660A KR 20050055660 A KR20050055660 A KR 20050055660A KR 1020050041399 A KR1020050041399 A KR 1020050041399A KR 20050041399 A KR20050041399 A KR 20050041399A KR 20050055660 A KR20050055660 A KR 20050055660A
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Abstract
본 발명은 미생물배양공정에 사용하는 2가-3가철염이 함유된 미네랄염이 함유된 미생물배양액 제조방법을 제시하는 것이 목적이다.An object of the present invention is to provide a method for preparing a microbial culture medium containing a mineral salt containing a divalent trivalent iron salt used in the microbial culture process.
이를 위하여 본 발명은, 2가철염과 3가철염을 혼합한 수용액을 자계(磁界)하에서 80∼100℃ 범위로 가열하면서 알칼리(Alkali)를 주입하여 pH를 8∼11로 조정하여 흑색의 자성산화철인 마그네타이트(Magnetite; Fe3O4)가 생성되면 침전, 여과 또는 탈수, 건조한 다음 착자기에서 착자(着磁)한다.To this end, the present invention, while heating the aqueous solution mixed with ferric salts and ferric salts in the range of 80 ~ 100 ℃ under a magnetic field (Alkali) is injected to adjust the pH to 8 to 11 black magnetic iron oxide When magnetite (Fe 3 O 4 ) is produced, it is precipitated, filtered or dehydrated, dried and then magnetized in a magnetizer.
여기서 보다 보자력(保磁力)이 높은 마그네타이트를 생산하기 위해서는 전술한 마그네타이트를 감마제2산화철(γ-Fe2O3)로 전환한 후에 이를 농염산(Conc-HCl) 수용액에 용해한 다음에 2가철염을 혼합하고, 80∼100℃ 범위로 가열하면서 알칼리를 주입하여 pH를 12∼14로 조정하여 흑색의 4,3산화철(Fe3O4)인 마그네타이트가 생성 되면 침전, 여과 또는 탈수, 건조한 다음 착자기에서 착자처리를 한다.In order to produce magnetite with higher coercive force, the above-described magnetite is converted to gamma ferric oxide (γ-Fe 2 O 3 ), and then dissolved in a concentrated hydrochloric acid (Conc-HCl) aqueous solution. Mix and inject alkali while heating to 80 ~ 100 ℃, adjust pH to 12 ~ 14, and produce black ferrous trioxide (Fe 3 O 4 ) magnetite. Precipitate, filter or dehydrate, dry and magnetizer. Process magnetization in
착자된 마그네타이트를 염산에 용해하고, 이에 착염을 생성하는 풀브산(Fulvic acid)을 주입한 다음에 중화제를 주입하여 pH를 6∼8로 중화처리를 하여 2가-3가철염용액을 제조한다.The magnetized magnetite is dissolved in hydrochloric acid, infused with fulvic acid (Fulvic acid), which forms a complex salt, and then neutralized with a neutralizer to prepare a bivalent trivalent iron solution.
그리고 착자된 마그네타이트에 마그네슘(Magnesium), 아연(Zn), 게르마늄 (Germanium), 칼슘 (Calcium), 셀레늄(Selenium)‥·등과 같은 유용미네랄성분이 다량 함유된 광물의 분말을 혼합한 것을 염산수용액에 용해하고, 이에 착염을 생성하는 풀브산을 주입한 다음에 중화제를 주입하여 pH를 6∼8로 중화처리를 하여 2가-3가철염이 함유된 미네랄염을 제조한다.The magnetized magnetite was mixed with mineral powder containing a large amount of useful minerals such as magnesium, zinc, germanium, calcium, selenium, etc. After dissolving and injecting fulvic acid to form a complex salt, a neutralizing agent is injected to neutralize the pH to 6 to 8 to prepare a mineral salt containing a divalent trivalent iron salt.
그리고 2가-3가철염이 함유된 미네랄염용액을 이용하여 미생물배양액을 제조한다.And microbial culture solution is prepared using a mineral salt solution containing divalent trivalent iron salt.
[색인어][Index]
자성산화철, 미네랄염, 마그네타이트(Magnetite), 감마제2산화철(γ-Fe2O3), 착자(着磁), 착염, 미생물배양액Magnetic iron oxide, mineral salt, magnetite, gamma ferric oxide (γ-Fe 2 O 3 ), magnetization, complex salt, microbial culture
Description
본 발명은 미생물배양공정에 사용하는 2가-3가철염이 함유된 미네랄염을 2가철염과 3가철염 혼합수용액을 80∼100℃로 가열하면서 알칼리(Alkali)를 주입하여 pH를 12∼14로 조정하여 흑색의 자성마그네타이트(Magnetite; Fe3O4)를 생성한 다음에 착자(着磁)하여 자화하고, 이를 염산용액에 용해한 다음에 풀브산(Fulvic acid)을 주입한 다음에 중화제를 주입하여 pH를 6∼8로 중화처리를 하여 2가-3가철염용액을 제조하는 방법과 착자된 마그네타이트에 마그네슘염(Magnesium salts), 아연염(Zinc salt), 셀레늄염(Selenium salt), 칼슘염(Calcium salt), 칼륨염(Potassium salt), 게르마늄(Germanium)과 같은 유용미네랄성분이 다량 함유된 광물의 분말을 혼합한 것을 염산에 용해하고, 이에 착염을 생성하는 풀브산을 주입한 다음에 중화제를 주입하여 pH를 6∼8로 중화처리를 하여 2가-3가철염이 함유된 미네랄염용액을 제조하는 방법, 2가-3가철염용액에 부식토와 광물의 분말을 혼합하여 1∼24개월간 숙성한 다음에 2가-3가철염이 함유된 미네랄염용액을 제조하는 방법에 관한 것이다.The present invention is a 12 to 14 pH by injecting alkali (Alkali) while heating the mixed solution of the ferric salt and the ferric salt salt 80 to 100 ℃ the mineral salt containing the divalent- trivalent iron salt used in the microbial culture process Black magnetite (Fe 3 O 4 ) was produced by magnetizing, and then magnetized by magnetization, dissolved in hydrochloric acid solution, and injected with fulvic acid, followed by neutralization. To neutralize the pH to 6 ~ 8 to prepare divalent trivalent iron solution, and magnesium salts, zinc salts, selenium salts and calcium salts in magnetized magnetite. A mixture of mineral powders containing a large amount of useful minerals such as Calcium salt, Potassium salt, and Germanium is dissolved in hydrochloric acid, followed by injection of fulvic acid to form a complex salt, followed by neutralizing agent. To neutralize the pH to 6-8 and divalent trivalent iron salt. Method for preparing a mineral salt solution containing the above, after mixing the ferric earth and mineral powder with divalent trivalent ferric salt solution for 1 to 24 months and then preparing a mineral salt solution containing divalent trivalent iron salt It is about a method.
일본 나가사키현(長崎縣)의 운젠(雲仙) 화산군의 북서쪽에 위치하는 가라고(唐比) 함몰습지(陷沒濕地)의 경우, 해양성 동식물의 사체와 화산회토가 혼합되어 퇴적된 부식토(이탄)는 자철광(마그네타이트)성분인 2가-3가철이 2.5∼5wt%, 기타 미네랄성분이 50∼70wt%, 유기물이 35∼45wt%로 존재한다.In the Kago Recession Wetlands, located northwest of the Unzen Volcanic Group in Nagasaki Prefecture, Japan, humus (pitan) deposited by the mixing of carcasses and volcanic ashes of marine flora and fauna Is 2.5 to 5 wt% of divalent trivalent iron (magnetite), 50 to 70 wt% of other mineral components, 35 to 45 wt% of organic matter.
일본 특허공보 평(平) 5-6619호의 폐수처리공정에서는 미생물배양공정에 전술한 부식토를 펠렛트(Pellet) 형태로 성형 가공한 것과 유문암(流紋岩) 또는 대사이드(Dacite)질 부석(Pumice)과 함께 생물반응기에 충전하여 적용한 결과 일반 생물학적(활성오니) 처리공정에 비해서 처리효율이 월등히 향상되었다.In the wastewater treatment process of Japanese Patent Publication No. Hei 5-6619, the above-mentioned humus soil is formed into pellets in the microbial culture process and rhyolite or dacite pumice ), And applied to the bioreactor, significantly improved the treatment efficiency compared to the general biological (active sludge) treatment process.
대한민국 특허 공개번호 2003-0019285호의 경우 전술한 부식토를 펠렛트 형태로 성형 가공한 것과 광물질을 함께 생물반응기에 충전하여 비육돈에 급여하는 음용수를 처리하여 급여한 결과 성장속도의 향상, 육질의 개선, 축사 내의 악취의 저감 ‥·등의 효과가 있었으며, 이를 산란계의 음용수를 처리하여 급여한 결과 산란율의 향상 및 계사 내의 악취저감 등의 효과가 있었다.In the case of Korean Patent Publication No. 2003-0019285, the above-mentioned humic soil was molded and processed into pellets, and the minerals were filled together with bioreactors and treated with drinking water for feeding pigs. There was an effect of reducing the odor inside, etc., and treatment of the drinking water of the laying hens resulted in the improvement of the laying rate and the reduction of the odor in the house.
그리고 자화된 2가-3가철염과 여러 미네랄염이 함유된 부식토의 경우 전술한 내용 이외에도 공기정화공정, 발효식품제조공정, 탈취처리공정, 토양개량제, 사료첨가제, 작물의 생육 및 발아촉진제, 화장품첨가제 등 다방면에 널리 이용되고 있다.In addition, in the case of humus soil containing magnetized divalent trivalent ferric salt and various mineral salts, in addition to the above, air purification process, fermented food manufacturing process, deodorization process, soil improving agent, feed additive, crop growth and germination promoter, cosmetic It is widely used in various fields such as additives.
그러나 나가사키현(長崎縣)의 가라고(唐比) 함몰습지(陷沒濕地)에서 출토되는 부식토의 경우는 습지보존 및 환경보존 측면에서 개발을 억제하고 있는 실정에 있으면서 상당히 고가로 판매되고 있어 구매조달 및 경제적인 문제점이 있으며, 또한 채굴위치 및 지점에 따라서 조성의 차이가 심하며, 2가-3가철 함량이 낮은 경우에는 효율이 월등히 감소하는 등의 문제점이 있다.However, the humus excavated from the Kago Recessed Wetlands in Nagasaki Prefecture is being sold at a very high price due to the fact that the development is suppressed in terms of wetland conservation and environmental conservation. There is a procurement and economic problems, and there is a serious difference in composition according to the location and location of the mining, there is a problem such that the efficiency is greatly reduced when the dihydric-3 iron content is low.
본 발명은 상기와 같은 문제점을 해소하기 위해 보자력이 높은 2가-3가철염이 함유된 미네랄염을 이용하여 미생물배양액을 제조방법을 제공하는 데 본 발명의 목적이 있는 것이다.The present invention has an object of the present invention to provide a method for producing a microbial culture liquid using a mineral salt containing a high covalent divalent trivalent iron salt to solve the above problems.
이와 같은 목적을 달성하기 위한 본 발명은, 제1철염과 제2철염을 혼합한 수용액을 가열하면서 알칼리를 주입하여 pH를 12 이상으로 조정하여 고순도의 마그네타이트(Fe3O4)를 생산되면 탈수건조한 후에 보자력이 최대한 높게 착자하여 자화 마그네타이트(Magnetite)의 제조단계, 자화된 마그네타이트를 염산에 용해하고, 이에 금속염과 착염을 생성하는 유기산을 주입한 다음에 알칼리로 중화하여 2가-3가철염용액의 제조단계, 필요한 미네랄을 함께 주입하여 2가-3가철염을 함유한 미네랄염용액의 제조단계, 2가-3가철염이 함유된 미네랄염을 이용하여 미생물배양액을 제조하는 단계로 이루어진 것에 특징이 있다.In order to achieve the above object, the present invention provides a high purity magnetite (Fe 3 O 4 ) to produce high purity magnetite (Fe 3 O 4 ) by injecting alkali while heating an aqueous solution mixed with ferrous and ferric salts, followed by dehydration and drying. Later, the coercive force is magnetized as high as possible to produce the magnetized magnetite, and the magnetized magnetite is dissolved in hydrochloric acid, and then, an organic acid that generates metal salts and complex salts is injected therein, followed by neutralization with an alkali to give a solution of the bivalent trivalent iron solution. The manufacturing step, the step of preparing the mineral salt solution containing the divalent trivalent iron salt by injecting the required minerals together, to prepare a microbial culture solution using the mineral salt containing the divalent trivalent iron salt. have.
본 발명은 미생물배양공정에 사용하는 2가-3가철염이 함유된 미네랄염을 이용하여 미생물배양액의 제조방법은 다음과 같다.The present invention is a method for producing a microbial culture liquid using a mineral salt containing a divalent trivalent iron salt used in the microbial culture process.
1. 자화 마그네타이트(Magnetite)의 제조단계1. Manufacturing Step of Magnetized Magnetite
2가철염과 3가철염의 혼합수용액을 80∼100℃로 가열하면서 알칼리(Alkali)를 주입하여 pH를 12∼14로 조정하여 흑색의 마그네타이트(Fe3O4)를 생성한다.Alkali is injected while the mixed aqueous solution of ferric and trivalent salts is heated to 80 to 100 ° C. to adjust the pH to 12 to 14 to produce black magnetite (Fe 3 O 4 ).
Fe2+ + 2Fe3+ + 8OH- → Fe3O4 + 4H2O...............................① Fe 2+ + 2Fe 3+ + 8OH - → Fe 3 O 4 + 4H 2 O ............................... ①
여기서 사용하는 2가철염은 제1황산철(FeSO4), 제l염화철(FeCl2)을 사용하고, 3가철염은 제2황산철(Fe2(SO4)3), 제2염화철(FeCl3)을 사용하며, 알칼리는 가성소다(NaOH), 암모니아수(NH4OH), 수산화칼륨(KOH), 탄산소다(Na2CO3)를 사용한다.The ferric salt used herein is ferrous sulfate (FeSO 4 ), ferric chloride (FeCl 2 ), and the ferric salt is ferric sulfate (Fe 2 (SO 4 ) 3 ), ferric chloride (FeCl) 3 ) is used, and alkali is used for caustic soda (NaOH), ammonia water (NH 4 OH), potassium hydroxide (KOH), sodium carbonate (Na 2 CO 3 ).
본 발명에서는 반응식①의 반응의 수율을 향상하기 위해서 전자석이 설치된 반응기의 자계(磁界) 하에서 교반반응을 한다.In the present invention, in order to improve the yield of the reaction of the reaction formula ①, the stirring reaction is performed under the magnetic field of the reactor in which the electromagnet is installed.
반응식①의 반응이 완료되면 반응과정에서 생성된 염을 제거하기 위해서 3∼4회 수세처리를 한 다음에 탈수 후에 건조한다.After the reaction of Scheme ① is completed, to wash the salt generated in the reaction process 3-4 times, and then dried after dehydration.
건조된 마그네타이트의 분발은 플라스틱(Plastic)병과 같은 캡슐(Capsule)에 압축-충전한 것을 착자기의 요크(Yoke)에 주입하고, 자속밀도가 350∼500G(Gauss) 로 착자처리 한다.The dried magnetite powder is injected into the yoke of the magnetizer by compression-filling a capsule such as a plastic bottle and magnetized to a magnetic flux density of 350 to 500 G (Gauss).
이때, 보다 보자력이 높은 마그네타이트를 생산하기 위해서는 반응식①에서 생성된 건조된 마그네타이트를 산소분위기 중에서 가열하여 감마산화2철(γ-Fe2O3)을 만들거나, 마그네타이트를 30∼35wt%의 과산화수소(H2O2)와 혼합하여 반응하면 감마산화2철(γ-Fe2O3)이 생성율이 향상된다.At this time, in order to produce a magnetite having a higher coercivity, the dried magnetite produced in the reaction equation ① is heated in an oxygen atmosphere to produce gamma ferric oxide (γ-Fe 2 O 3 ), or the magnetite is 30 to 35wt% hydrogen peroxide ( When reacted with H 2 O 2 ), the production rate of gamma ferric oxide (γ-Fe 2 O 3 ) is improved.
다음에 이 감마산화2철(γ-Fe2O3)을 염산용액에 용해한 다음에 전자석이 설치된 반응기의 자계하에서 80∼100℃로 가열하면서 알칼리(Alkali)를 주입하여 pH를 12∼14로 조정하면 흑색의 마그네타이트(Fe3O4)를 재생성한다.Next, the ferric gamma oxide (γ-Fe 2 O 3 ) is dissolved in a hydrochloric acid solution, and alkali (Alkali) is injected while heating to 80-100 ° C. under a magnetic field of a reactor equipped with an electromagnet to adjust the pH to 12-14. The black magnetite (Fe 3 O 4 ) is regenerated.
4Fe3O4 + O2 → 6γ- Fe2O3 ........................................②4Fe 3 O 4 + O 2 → 6γ-Fe 2 O 3 ..................... .... ②
2Fe3O4 + H2O2 → 3γ - Fe2O3 + H2O ...............................③2Fe 3 O 4 + H 2 O 2 → 3γ-Fe 2 O 3 + H 2 O .............................. .③
γ-Fe2O3 + 6HCl → 2FeCl3 + 3H2O .................................④γ-Fe 2 O 3 + 6HCl → 2FeCl 3 + 3H 2 O ................................. ④
FeCl2 + 2FeCl3 + 8NaOH → Fe3O4 + 8NaCl + 4H2O .....................⑤FeCl 2 + 2FeCl 3 + 8NaOH → Fe 3 O 4 + 8NaCl + 4H 2 O .......................... ⑤
FeCl2 + 2FeCl3 + 8NH3 + 4H2O → Fe3O4 + 8NH4Cl .....................⑥FeCl 2 + 2FeCl 3 + 8NH 3 + 4H 2 O → Fe 3 O 4 + 8NH 4 Cl ..................... ⑥
반응식⑤ 또는 ⑥의 반응이 완료되면 반응과정에서 생성된 염을 제거하기 위해서 3∼4회 수세처리를 한 다음에 탈수 후에 건조한다.After completion of the reaction of reaction formula ⑤ or ⑥, to remove the salt generated in the reaction process, it is washed three to four times and then dried after dehydration.
건조된 마그네타이트의 분발은 플라스틱 병과 같은 캡슐에 압축-충전한 것을 착자기의 요크(Yoke)에 주입하고, 자속밀도가 450∼500G로 착자한다.The dried magnetite powder is injected into the yoke of the magnetizer by compression-filling a capsule such as a plastic bottle and magnetized to a magnetic flux density of 450 to 500 G.
2. 2가-3가철염용액의 제조단계2. Preparation Step of Divalent-3 Ferric Salt Solution
착자된 자화마그네타이트분말을 농염산수용액에 용해한다.The magnetized magnetite magnetite powder is dissolved in concentrated hydrochloric acid solution.
Fe3O4 + 8HCl →FeCl2 + 2FeCl3 + 4H2O ..............................⑦Fe 3 O 4 + 8HCl → FeCl 2 + 2FeCl 3 + 4H 2 O ............................. ⑦
반응식⑦의 반응이 완료되면 풀브산(Fulvic acid)과 2가·3가 철염의 혼합 비율은 풀브산/2가·3가 철염을 무게 비(건량 기준)로 0.8∼1.2의 범위로 혼합·교반한 다음에 알칼리(NaOH, KOH, Na2CO3, Ca(OH)2, CaO… 등)를 주입하여 pH를 6∼8로 중화하여 2가-3가철염용액을 만든다.When the reaction of Scheme ⑦ is completed, the mixing ratio of fulvic acid and divalent and trivalent iron salts is mixed and stirred in the range of 0.8-1.2 by weight ratio (dry basis) of fulvic acid / divalent and trivalent iron salts. Then, alkali (NaOH, KOH, Na 2 CO 3 , Ca (OH) 2 , CaO ... etc.) is injected to neutralize the pH to 6-8 to form a bivalent trivalent iron solution.
풀브산(Fulvic acid) + 2가·3가 철염 + 알칼리 → 풀브산철착염(Complex iron fulvate; 2가-3가철염) + H2O ……………………………………………………⑧Fulvic acid + divalent and trivalent iron salts + alkali → Complex iron fulvate + H 2 O. … … … … … … … … … … … … … … … … … … … ⑧
풀브산(Fulvic acid)은 천연의 부식물질(이탄)을 1차 가성소다(NaOH)와 같은 알칼리(Alkali)를 처리하여 pH를 13∼14로 조정하여 용해된 용액에 염산(HCl)과 같은 강산을 주입하여 pH를 1∼2로 조정하였을 때 침전되는 부식산(Humic acid)을 제거하고 난 다음에 용액상태로 추출한다. Fulvic acid is treated with alkali, such as primary caustic soda (NaOH), to adjust the pH to 13-14 by treating natural corrosive substances (peat) with strong acids such as hydrochloric acid (HCl) in the dissolved solution. After the pH was adjusted to 1-2, the precipitated humic acid was removed, and then extracted in solution.
이때 경비절감을 위해서 전술한 반응식⑦의 반응에서 인공제조된 자화마그네타이트분말 대신 천연의 자철광(磁鐵鑛)을 300∼400메시(Mesh)로 분쇄한 분말을 사용할 수도 있다.At this time, instead of artificially manufactured magnetite magnetite powder in the reaction of Reaction Formula ⑦ described above, it is also possible to use a powder obtained by grinding natural magnetite with 300 to 400 mesh.
3. 2가-3가철염을 함유한 미네랄염용액의 제조단계3. Preparation of Mineral Salt Solution Containing Divalent-3 Ferric Salts
자화된 마그네타이트의 분말이나 자화도가 높은 천연의 자철광을 분쇄한 분말에 마그네슘염(Magnesium salts), 아연염(Zinc salt), 셀레늄염(Selenium salt), 칼슘염(Calcium salt), 칼륨염(Potassium salt), 게르마늄(Germanium)과 같은 미네랄성분이나 유용미네랄성분이 다량 함유된 광물의 분말을 염산수용액에 용해하고, 전술한 금속염과 착염을 생성하는 풀브산을 풀브산/미네랄염을 무게 비(건량 기준)로 0.8∼1.2의 범위로 혼합·교반한 다음에 알칼리(NaOH, KOH, Na2CO3, Ca(OH)2, CaO… 등)를 주입하여 pH를 6∼8로 중화하여 유용미네랄염이 함유된 2가-3가철용액을 제조한다.Magnesium salts, zinc salts, selenium salts, calcium salts, potassium salts (Potassium) in powder of magnetized magnetite or natural magnetite with high magnetization salt), mineral powders such as germanium (Germanium) and mineral powders containing a large amount of useful minerals are dissolved in aqueous hydrochloric acid solution, fulvic acid / mineral salt to fulvic acid to produce the complex salt and the above-mentioned metal salt After mixing and stirring in the range of 0.8 to 1.2, alkali (NaOH, KOH, Na 2 CO 3 , Ca (OH) 2 , CaO…, etc.) is injected to neutralize the pH to 6-8. This containing divalent trivalent iron solution was prepared.
4. 미생물배양액제조단계4. Microbial culture solution production step
2가-3가철염을 함유한 미네랄염용액의 제조단계에서 제조된 2가-3가철염을 함유한 미네랄염수용액에 금속이온과 결합할 수 있는 관능기(官能基)가 있으면서 배양액으로 사용할 수 있는 덱스트란(Dextran), 젤라틴(Gelatin), 콜라겐(Collagen), 글루타민(Glutamine), 리신(Lysine), 폴리리신(Poly-L-Lysine), 글리신(Glycine), 키토산(Chitosan), 알브민(Albumin), 한천(Agar), 당밀(Molasses)을 2종류 이상 혼합한 것을 물에 용해한 20∼30wt%의 수용액에 2가-3가철염을 함유한 미네랄염용액을 건량 기준으로 00.1∼10wt%로 주입하고 50∼80℃ 범위로 30∼40분간 30∼40분간 가열반응 후 상온으로 냉각하여 미생물배양액을 제조한다.The mineral salt solution containing the divalent trivalent iron salt prepared in the step of preparing the mineral salt solution containing the divalent trivalent iron salt has a functional group capable of binding with metal ions and can be used as a culture medium. Dextran, Gelatin, Collagen, Glutamine, Lysine, Poly-L-Lysine, Glycine, Chitosan, Albumin ) A mixture of two or more types of agar, molasses, molasses and molasses is injected into a solution of 20 to 30 wt% dissolved in water at 00.1 to 10 wt% of the mineral salt solution containing divalent trivalent iron salt. After heating for 30 to 40 minutes in a range of 50 to 80 ° C. for 30 to 40 minutes, the mixture is cooled to room temperature to prepare a microbial culture solution.
이상에서 상술한 바와 같이 본 발명은, 2가-3가철염을 함유한 미네랄염용액은 미생물배양공정에 널리 보급될 것으로 전망된다.As described above, in the present invention, the mineral salt solution containing the divalent trivalent iron salt is expected to be widely used in the microbial culture process.
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| KR101588441B1 (en) * | 2015-07-22 | 2016-02-03 | 정재락 | Method for preparing organic selenium |
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| KR101588441B1 (en) * | 2015-07-22 | 2016-02-03 | 정재락 | Method for preparing organic selenium |
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