KR20050053071A - Functional food composition comprising the polysaccharide and oligosaccharide extract of laminaria for improvement and prevention of constipation - Google Patents

Abstract

The present invention relates to a functional food composition containing an extract of kelp polysaccharide and oligosaccharide obtained by acid and high temperature heating as an active ingredient. The kelp polysaccharide and oligosaccharide of the present invention promotes the movement of food in the small intestine and promotes the movement of the intestine. Since it exhibits the effect of smoothing bowel movements, it can be used in health functional foods such as functional drinks as a functional food composition for improving constipation and prevention.

Description

Functional food composition comprising the polysaccharide and oligosaccharide extract of Laminaria for improvement and prevention of constipation

The present invention relates to a functional food composition for improving and preventing constipation, containing the extract of kelp polysaccharide and oligosaccharide obtained by acid and high temperature heating as an active ingredient.

In recent years, obesity, which is a major cause of adult diseases due to excessive intake of animal fat, lack of exercise, and excessive drinking, has been increasing rapidly (Ministry of Health and Welfare, 1997 National Nutrition Survey Report, p37, 1997). Therefore, the incidence of constipation due to lack of intake of dietary fiber has been greatly enlarged, and it is also pointed out that this is a cause of adult disease.

Dietary fiber is widely distributed in plant foods, and eating habits are changing from sugar-based foods to protein and lipid-based foods. This lack of dietary fiber is inevitably replaced by other foods or processed products, the seaweed polysaccharide has recently attracted attention.

Seaweed polysaccharides come in a variety of varieties, but their functions have recently been recognized around alginic acid and are widely used as processed food materials.

Seaweeds are spotlighted as a health food material with functions of lowering lipids, lowering cholesterol, improving constipation, and releasing heavy metals, and there is increasing interest in their proper use, and development of various health functional products is actively progressing. have. In recent years, the development of seaweed oligosaccharide manufacturing technology capable of industrial production has made a lot of technical approaches to the use of seaweed as a functional food material, but it is still considered to be a lot of research to commercialize (Beaumont JL. Et al., Bull WHO , 43 , pp891-897, 1970). Recently, functional seaweed beverages containing trace amounts of seaweed polysaccharides have been introduced, but they are not based on seaweed, and in the case of Japan, various products using seaweed have been developed, but remain at the level of simple processing. Therefore, when reviewing these various conditions, it seems that there is still plenty of possibility of developing functional materials and products using seaweed.

Kelp (Laminaria) is a genus of seaweed kelp and kelp, a traditional seaweed food. It is distributed in the Pacific coasts of Korea, Japan, Kamchatka Peninsula, Sakhalin Island, etc., and the size is 1.5 to 3.5 m, and the butterfly is 25 to 40 cm. Kelp plants grow over 20 species on the Pacific coast, and there are also large varieties of more than 10 m. The main species are L. japonica, L. ochotensis, and L. religiosa. have. In addition to various pigments such as carotene, xanthophylls, and chlorophyll, kelp contains a lot of carbohydrates, such as manit and laminarin, which are made by carbon assimilation, and alginic acid, which is a component of cell walls. This contains.

Although the constituents of kelp vary depending on the type, they are generally 16% moisture, 7% protein, 1.5% fat, 49% carbohydrates, and 26.5% inorganic salts. 20% of carbohydrates are fibrin and the rest are polysaccharides such as alginic acid and laminarin. In particular, many inorganic salts, such as iodine, potassium, and calcium, are contained.

Meanwhile, Korean Patent Application No. 1991-011992 (Publication 93-1825) discloses a method for preparing a beverage using seaweed and kelp, and Korean Patent Application No. 1995-015089 discloses a method for preparing juice containing seaweed ear as a main ingredient. It is disclosed in relation to the present invention, but the texture is not good by the method of preparing a drink or juice using the kelp itself, and the unique fishy taste and aroma are strong, so that it is unsuitable as a drink.

Accordingly, the present inventors completed the present invention by examining the effects of the tangle polysaccharide and oligosaccharide extract on the exercise of organs while searching for various bioactive effects of kelp.

It is an object of the present invention to provide a functional food composition for improving constipation and preventing constipation containing an extract of kelp polysaccharide and oligosaccharide obtained by acid and high temperature heating as an active ingredient.

In order to achieve the above object, the present invention provides a functional food composition for constipation improvement and prevention containing the kelp oligosaccharide extract obtained by acid and high temperature heating treatment as an active ingredient.

The kelp is L. japonica , L. ochotensis , L. religiosa , and the like.

The acid treatment means treatment with weak acids, preferably citric acid, such as citric acid, acetic acid and malic acid.

The high temperature treatment includes heat treatment at 100 to 150 ° C., preferably at 120 ° C. for 10 minutes to 5 hours, preferably 60 minutes.

The kelp polysaccharides and oligosaccharides are characterized in that they are mainly alginic acid oligosaccharides.

Hereinafter, the present invention will be described in detail.

The kelp polysaccharide and oligosaccharide extract of the present invention is lyophilized after lyophilization and pulverized, and then the volume of citric acid, acetic acid, malic acid, etc., which is about 3 to 60 times the dry weight of kelp, preferably about 20 to 40 times Hydrothermal extraction, cold extraction, reflux cooling extraction or ultrasonic extraction, for about 10 minutes to 5 hours, preferably 60 minutes, at an extraction temperature of 120 to 150 ° C., preferably 120 ° C., with a weak acid, preferably 0.5% citric acid solution. Extraction method, preferably obtained by continuous extraction once to 5 times, preferably 2 to 3 times by hot water extraction, and then filtered under reduced pressure and the filtrate is 20 to 100 ℃, preferably 50 to 70 with a vacuum rotary concentrator Concentration under reduced pressure at ℃ can be obtained the polysaccharide and oligosaccharide extract of the present invention.

The present invention provides a functional food composition for constipation improvement and prevention containing the extracts of kelp polysaccharide and oligosaccharide obtained by acid and high temperature heating treatment in the above method.

Kelp polysaccharide and oligosaccharide extract obtained from the above method was confirmed through various experiments to promote the movement speed of the food in the small intestine, promote the movement of the intestine to facilitate bowel movement.

Functional food composition for constipation improvement and prevention of the present invention comprises the extract in an amount of 0.02 to 90% by weight based on the total weight of the composition.

In addition, the present invention provides a dietary supplement containing the kelp extract, and a food supplement acceptable food supplement additive exhibiting a constipation prevention and improvement effect.

The kelp polysaccharide and oligosaccharide extract of the present invention has almost no toxicity and side effects, and therefore can be used with confidence even for prolonged administration.

Therefore, the extract of the present invention can be added to food or beverages for the purpose of improving constipation and preventing. Examples of the food to which the extract can be added include various foods, beverages, gums, teas, vitamin complexes, and health functional foods. At this time, the amount of the extract in the food or beverage, that is, in general, the health functional composition of the present invention can be added to 0.01 to 15% by weight of the total food weight, the health beverage composition is 0.02 to 10 g, based on 100 ml, Preferably it can be added in the ratio of 0.3-1 g.

The health beverage composition of the present invention has no particular limitation on the liquid component except for containing the extract as an essential ingredient in the ratios indicated, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract, for example rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. . The proportion of such natural carbohydrates is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. The compositions of the present invention may also contain pulp for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected from the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

The present invention is described in more detail based on the following examples, although the present invention is not limited thereto.

Example 1 Preparation of Kelp Oligosaccharide Extract

Water washed and dried with seaweed purchased from a market near the East Sea, dried, and 100 ml of kelp was added to 3 l of 0.5% citric acid solution and heated and extracted at 120 ° C for 60 minutes. And 5 liters of oligosaccharide extract.

Example 2. Preparation of Kelp Beverage

A kelp drink was prepared by adding a predetermined ratio of the beverage additives of the ingredients shown in Table 1 to the kelp polysaccharide and oligosaccharide extracts obtained in Example 1.

Material and Additives content (%) Kelp polysaccharide and oligosaccharide extract of Example 1 100 Chondroitin Sulfate 0.5 glucose 4 honey One Apple Extract Concentrate (50 Brix) 4 Angelica Extract One Vitamin c 0.001

Experimental Example 1. Effect of Kelp Polysaccharide and Oligosaccharide Extract on Small and Small Intestine Exercise

1-1. Effect on Small Intestine Movement in Laboratory Animals

        In order to examine the effects of the seaweed polysaccharide and oligosaccharide extracts obtained in Example 1 on small intestine movement, 20-25 g of male rats (mouse, received from Korea Experimental Animal Center) that were fasted for 20 hours as experimental animals 10 One group was divided into three groups, and then oral administration of the kelp polysaccharide and oligosaccharide extract of Example 1 and the kelp drink of Example 2 to each group with a normal diet group ingested a normal diet to each group 1 After time, oral administration of charcoal meal (charcoal meal 50%, arabia rubber suspension) at 0.2 ml / mouse, and after 30 minutes of death, laparotomy and extraction from the pyloric region just before the cecum, (cm) was measured, and the effect of the small intestine exercise was compared by the following equation (1).

As a result, as shown in Table 2, in the normal diet group, the movement distance of chagol mill was 63.4%, which was 73.6% by the administration of the kelp polysaccharide and oligosaccharide extract of Example 1, which was further moved by about 20%. In the case of the kelp drink of Example 2, the moving distance was 78.2%, which was 24% more than that of the normal diet group.

As a result, it was found that polysaccharide and oligosaccharide extracts can increase the bowel effect by promoting the movement of food in the small intestine.

group Dosage (mg / kg) motility(%) Normal diet. 63.4 ± 2.34 ^g Kelp polysaccharide and oligosaccharide extract of Example 1 10 73.6 ± 2.19 ^a Kelp drink of Example 2 10 78.2 ± 2.49 ^b  1) The numerical value represents the mean ± S.D. (n = 10). 2) Numbers marked with the same initials were not significantly different from each other by Duncan's multi-range test (P <0.05)

1-2. Effect on ileal movement of experimental animals

In order to determine the effect of the kelp polysaccharide and oligosaccharide extract obtained in Example 1 on the ileal motility, 10 ml / of the kelp polysaccharide and oligosaccharide extract of Example 1 and the kelp drink of Example 2 to the rabbit as an experimental animal kg to expose the small intestine, cut about 30 cm of ileum from 10-15 cm at the junction of the cecum and small intestine, dip it into a Tyroid solution, and use the syringe to The ileum specimens were washed out, and the ileum was cut into 2.0-2.5 cm lengths and organ bath containing a thyroid solution saturated with 37 ° C. and 95% oxygen (O ₂ ) to 5% carbon dioxide (CO ₂ ) mixed gas. Suspension in the organ bath), and after stabilizing the extracted sample, the test sample was added to express the change in the degree of isotonic shrinkage in% of the control (co) test.

As a result, as shown in Table 3 below, the contractile force of the intestinal tract of the rabbit was increased in dose-dependent administration of the kelp polysaccharide and oligosaccharide extract of Example 1 and the kelp drink of Example 2. This is considered evidence that the kelp polysaccharide and oligosaccharide extract has an effect of stimulating the exercise of the intestine by stimulating the intestine of the rabbit.

Concentration (mg / ml) Shrinkage (%) Kelp polysaccharide and oligosaccharide extract of Example 1 Kelp drink of Example 2 1 0 ^-2 2.46 ± 0.33 ^d 7.47 ± 0.76 ^d 3 × 0 ^-2 3.51 ± 0.21 ^c 9.88 ± 0.17 ^c 10 ^-1 5.17 ± 0.09 ^b 10.4 ± 1.48 ^c 3 × 0 ^-1 5.03 ± 0.11 ^b 15.7 ± 1.54 ^b One 6.13 ± 0.15 ^a 23.0 ± 1.39 ^a  1) The numerical value represents the mean ± S.D. (N = 10). 2) Numbers marked with the same initials were not significantly different from each other by Duncan's multi-range test (P <0.05)

As a result, kelp polysaccharide and oligosaccharide extract may promote the movement of food in the small intestine and promote bowel movements to facilitate bowel movements, which may contribute to the prevention and improvement of constipation and further prevention of various adult diseases in the body. It is thought to be.

Examples of the formulation of the composition are described below, but are not intended to limit the present invention but to explain in detail only.

Formulation Example 1 Preparation of Healthy Food

Kelp polysaccharide and oligosaccharide extract of Example 1. 1000 mg

Vitamin Blend ...

Vitamin A Acetate ............... 70 μg

Vitamin E .................................. 1.0 mg

Vitamin B1 ..................... 0.13 mg

Vitamin B2 ..................... 0.15 mg

Vitamin B6 ......................................... 0.5 mg

Vitamin B12 ................................. 0.2 μg

Vitamin C ......................................... 10 mg

Biotin ......................................... 10 μg

Nicotinic Acid Amide ... 1.7 mg

Folic acid ......................................... 50 ㎍

Calcium Pantothenate ..................... 0.5 mg

Inorganic Mixture ...

Ferrous Sulfate ............... 1.75 mg

Zinc Oxide ............... 0.82 mg

Magnesium Carbonate ......................................... 25.3 mg

Potassium monophosphate ......................................... 15 mg

Dicalcium Phosphate Diluent ... 55 mg

Potassium Citrate ............... 90 mg

Calcium Carbonate ... 100 mg

Magnesium Chloride ......................................... 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixtures is mixed with a component suitable for a health food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method. The granules may be prepared and used for preparing a health food composition according to a conventional method.

Formulation Example 2 Preparation of Healthy Drinks

Seaweed polysaccharide and oligosaccharide extract of Example 1 ...... 1000 mg

Citric Acid ......................................... 1000 mg

Oligosaccharide ......................................... 100 g

Plum concentrate ........................... 2 g

Taurine ......................................... 1 g

Purified water is added ................... 900 ml

After mixing the above components in accordance with the conventional healthy beverage manufacturing method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and refrigerated Used to prepare the healthy beverage composition of the invention.

Although the composition ratio is mixed with a relatively suitable component for a preferred beverage in a preferred embodiment, the compounding ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and usage.

As described above, the kelp polysaccharide and oligosaccharide extract obtained by the acid and high temperature heating treatment of the present invention promotes the movement speed of the food in the small intestine, promotes the movement of the intestine, and facilitates bowel movement, thereby improving constipation and preventing functional food. As a composition, it can be used for health functional foods, such as a functional drink.

Claims (8)

Functional food composition for constipation improvement and prevention containing the kelp polysaccharide and oligosaccharide extract obtained by acid and high temperature heating treatment as an active ingredient. The functional food composition of claim 1, wherein the kelp is L. japonica . The functional food composition according to claim 1, wherein the acid is treated with citric acid. The functional food composition of claim 1, wherein the high temperature treatment is performed at 100 to 150 ° C. for 10 minutes to 5 hours. The functional food composition of claim 1, wherein the kelp polysaccharide and oligosaccharide are mainly alginic acid oligosaccharides. The functional food composition of claim 1, comprising 0.02 to 90% by weight of said extract relative to the total weight of the composition. A dietary supplement comprising the extract of kelp polysaccharide and oligosaccharide of claim 1, and a food supplement acceptable food supplement, which has a constipation prevention and improvement effect. The health functional food according to claim 7, which is a health beverage.