KR20050053068A - Functional food composition comprising the polysaccharide and oligosaccharide extract of laminaria for lowering of the lipids in blood serum and cholesterol - Google Patents

Abstract

The present invention relates to a functional food composition containing the seaweed polysaccharide and oligosaccharide extract obtained by acid and high temperature heating as an active ingredient, the kelp polysaccharide and oligosaccharide of the present invention not only regulate cholesterol and arteriosclerosis index in serum, but also neutral lipid And because it shows an effect of reducing the content of phospholipids, it can be used in health functional foods such as functional health drinks, such as functional food composition for reducing serum lipids and cholesterol.

Description

Functional food composition comprising the polysaccharide and oligosaccharide extract of Laminaria for lowering of the lipids in blood serum and cholesterol}

The present invention relates to a functional food composition for lowering serum lipids and cholesterol containing an extract of kelp polysaccharide and oligosaccharide obtained by acid and high temperature heating as an active ingredient.

Recently, as the standard of living improves, the hygiene environment is improved and the dietary life is westernized, and the average life span is extended. Thus, geriatric disease has emerged as the biggest medical challenge today. Recently, about one-third of total deaths in Korea are caused by circulatory diseases, and atherosclerotic circulatory diseases account for the largest proportion. In Korea, cerebral vascular diseases such as cerebral hemorrhage and cerebral infarction due to hypertension, and coronary artery disease due to hyperlipidemia in developed countries in Gumi, have recently increased incidence of hyperlipidemia due to increased intake of animal fat in Korea. Ischemic heart and brain diseases are increasing on the basis of the Ministry of Health and Welfare, 1997 National Nutrition Survey Report , p37, 1997. Risk factors that cause such atherosclerosis include hyperlipidemia, hypertension, smoking, diabetes, urinary acidosis, obesity, lack of exercise, and excessive drinking (Kannel WB., Med. Clin. North. Am , 79 , pp951-). 956, 1995; Grodstein F., N. Engl. J. Med. , 336 , pp1769-1774, 1997), hyperlipidemia, smoking, and hypertension are known as three major risk factors in Western countries, but in Korea, hypertension, smoking, Diabetes was the main risk factor. However, hyperlipidemia has recently emerged as a major risk factor. In Korea, the prevalence and mortality of atherosclerosis and its associated cardiovascular disease is increasing, and considering the increase in the prevalence of hyperlipidemia, one of the causes, it is urgently required to take measures for proper prevention and treatment of hyperlipidemia. As a main method for preventing cardiovascular diseases, various methods such as avoiding excessive intake of calories and limiting the amount and type of ingested fat have been proposed, and in recent years, proper intake of natural foods has become important in cardiovascular diseases. From this point of view, the active substance for reducing hyperlipidemia, which is a major cause of pulmonary disease, has been actively pursued in various fields.

In addition, elevated plasma cholesterol levels are considered one of the most important risk factors for atherosclerosis.

Cholesterol is produced mainly in the liver and is present in the blood in small round particles called lipoproteins, which carry cholesterol throughout the body. There are two types of lipoproteins, low density lipoprotein (LDL) and high density lipoprotein (HDL). HDL carries cholesterol from other tissues. On the other hand, LDL is produced mainly in the liver and carries cholesterol to other tissues of the human body, so a large amount of LDL accumulates a lot of cholesterol in blood vessels to promote atherosclerosis, and about 70% of blood cholesterol is present as harmful cholesterol (LDL). Kim, Kyoung-hwan, Lee Woo-joo, Pharmacology Lecture, 4th edition, p466-481, 2001).

In fact, cholesterol is an essential ingredient necessary for the normal functioning of the human body, nutrients necessary to make cells, and a component of various hormones such as corticosteroids, male hormones, and female hormones. It can help you digest fatty foods. In addition, it is a nutrient necessary for the human body, such as a component of a biological membrane and used as a starting material for hormonal synthesis. However, when too much cholesterol is accumulated in blood vessels, it is known to cause heart disease. So far, there is no preventive method other than low cholesterol diet, and taking drugs such as cholesterol-lowering drugs are effective, but their use is extremely limited because of causing side effects such as liver dysfunction due to inhibition of cholesterol synthase.

Chitosan, phytosterol, inositol, and pectin are known substances that lower cholesterol in the blood, but the effects or metabolic mechanisms are not clear when phytosterol is excluded. . Phytosterol, a plant sterol, has a known mechanism of inhibiting metabolism of cholesterol in the human body through competition with low-density lipoprotein (LDL) -cholesterol, which is harmful to human body due to its structural similarity to cholesterol. It is FDA approved.

The cause of the rise in plasma cholesterol level is a hereditary disease.In this case, in critically ill patients, cholesterol therapy inhibitors, nicocomol, clofibrate, ion exchange resins, anabolic steroids, etc. Although the drug is used, these drugs have side effects such as hepatotoxicity, gastrointestinal disorder, carcinogenicity.

Another major cause of elevated plasma cholesterol levels is the excessive intake of fat by eating a diet that consumes large amounts of eggs, butter, meat, and the like. In the case of dietary hypercholesterolemia, it usually does not lead to severe hypercholesterolemia, but it is a problem that cholesterol accumulates in blood vessels from a young age and causes arteriosclerosis in adults. There is a risk of causing. In regard to this type of hyperlipidemia, dietary therapy in which lipid intake is limited to an appropriate range is more important than problem-based drug therapy. However, since dietary restrictions involve mental pain and deprivation of the enjoyment of eating, strict enforcement is difficult and the effect is often limited. Therefore, the development of foods with a cholesterol-lowering action is required.

Seaweeds are spotlighted as a health food material with functions of lowering lipids, lowering cholesterol, improving constipation, and releasing heavy metals, and there is increasing interest in their proper use, and development of various health functional products is actively progressing. have. In recent years, the development of seaweed oligosaccharide manufacturing technology capable of industrial production has made a lot of technical approaches to the use of seaweed as a functional food material, but it is still considered to be a lot of research to commercialize (Beaumont JL. Et al., Bull WHO , 43 , pp891-897, 1970). Recently, functional seaweed beverages containing trace amounts of seaweed polysaccharides have been introduced, but they are not based on seaweed, and in the case of Japan, various products using seaweed have been developed, but remain at the level of simple processing. Therefore, when reviewing these various conditions, it seems that there is still plenty of possibility of developing functional materials and products using seaweed.

Kelp (Laminaria) is a genus of seaweed kelp and kelp, a traditional seaweed food. It is distributed in the Pacific coasts of Korea, Japan, Kamchatka Peninsula, Sakhalin Island, etc., and the size is 1.5 to 3.5 m, and the butterfly is 25 to 40 cm. Kelp plants grow over 20 species on the Pacific coast, and there are also large varieties of more than 10 m. The main species are L. japonica, L. ochotensis, and L. religiosa. have. In addition to various pigments such as carotene, xanthophylls, and chlorophyll, kelp contains a lot of carbohydrates, such as manit and laminarin, which are made by carbon assimilation, and alginic acid, which is a component of cell walls. This contains.

Although the constituents of kelp vary depending on the type, they are generally 16% moisture, 7% protein, 1.5% fat, 49% carbohydrates, and 26.5% inorganic salts. 20% of carbohydrates are fibrin and the rest are polysaccharides such as alginic acid and laminarin. In particular, many inorganic salts, such as iodine, potassium, and calcium, are contained.

Meanwhile, Korean Patent Application No. 1991-011992 (Publication 93-1825) discloses a method for preparing a beverage using seaweed and kelp, and Korean Patent Application No. 1995-015089 discloses a method for preparing juice containing seaweed ear as a main ingredient. It is disclosed in relation to the present invention, but the texture is not good by the method of preparing a drink or juice using the kelp itself, and the unique fishy taste and aroma are strong, so that it is unsuitable as a drink.

Therefore, the present inventors completed the present invention by identifying serum lipid and cholesterol lowering effects while searching for various bioactive effects of kelp.

It is an object of the present invention to provide a functional food composition for lowering serum lipids and cholesterol containing an extract of kelp polysaccharide and oligosaccharide obtained by acid and high temperature heating as an active ingredient.

In order to achieve the above object, the present invention provides a functional food composition for lowering serum lipids and cholesterol containing an extract of kelp polysaccharide and oligosaccharide as an active ingredient obtained by acid and high temperature heating treatment.

The kelp is L. japonica , L. ochotensis , L. religiosa , and the like.

The acid treatment means treatment with weak acids, preferably citric acid, such as citric acid, acetic acid and malic acid.

The high temperature treatment includes heat treatment at 100 to 150 ° C., preferably at 120 ° C. for 10 minutes to 5 hours, preferably 60 minutes.

The kelp polysaccharides and oligosaccharides are characterized in that they are mainly alginic acid oligosaccharides.

Hereinafter, the present invention will be described in detail.

The kelp polysaccharide and oligosaccharide extract of the present invention is lyophilized after lyophilization and pulverized, and then the volume of citric acid, acetic acid, malic acid, etc., which is about 3 to 60 times the dry weight of kelp, preferably about 20 to 40 times Hydrothermal extraction, cold extraction, reflux cooling extraction or ultrasonic extraction, for about 10 minutes to 5 hours, preferably 60 minutes, at an extraction temperature of 120 to 150 ° C., preferably 120 ° C., with a weak acid, preferably 0.5% citric acid solution. Extraction method, preferably obtained by continuous extraction once to 5 times, preferably 2 to 3 times by hot water extraction, and then filtered under reduced pressure and the filtrate is 20 to 100 ℃, preferably 50 to 70 with a vacuum rotary concentrator Concentration under reduced pressure at ℃ can be obtained the polysaccharide and oligosaccharide extract of the present invention.

The present invention provides a functional food composition for lowering serum lipids and cholesterol containing kelp polysaccharide and oligosaccharide extract obtained by acid and high temperature heating treatment in the above manner.

It was confirmed through various experiments that the polysaccharide and oligosaccharide extracts obtained from the above method have the effect of reducing cholesterol and arteriosclerosis in serum as well as reducing the content of neutral lipid and phospholipid.

Functional food composition for lowering serum lipids and cholesterol of the present invention comprises the extract in an amount of 0.02 to 90% by weight based on the total weight of the composition.

In addition, the present invention provides a dietary supplement containing the kelp polysaccharide and oligosaccharide extract, and a food supplement acceptable food supplement, which exhibit serum lipid and cholesterol lowering effects.

The kelp polysaccharide and oligosaccharide extract of the present invention has little toxicity and side effects, and thus is a drug that can be used with confidence even when taken for long periods of time.

Therefore, the extract of the present invention may be added to food or beverage for the purpose of lowering serum lipids and cholesterol. Examples of the food to which the extract can be added include various foods, beverages, gums, teas, vitamin complexes, and health functional foods. At this time, the amount of the extract in the food or beverage, that is, in general, the health functional composition of the present invention can be added to 0.01 to 15% by weight of the total food weight, the health beverage composition is 0.02 to 10 g, based on 100 ml, Preferably it can be added in the ratio of 0.3-1 g.

The health beverage composition of the present invention has no particular limitation on the liquid component except for containing the extract as an essential ingredient in the ratios indicated, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract, for example rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. . The proportion of such natural carbohydrates is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. The compositions of the present invention may also contain pulp for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected from the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

The present invention is described in more detail based on the following examples, although the present invention is not limited thereto.

Example 1 Preparation of Kelp Polysaccharide and Oligosaccharide Extract

After washing with water, dried and dried kelp from the market near the East Sea, 3 liters of 0.5% citric acid solution was added to 100 g of kelp and heated and extracted at 120 ℃ for 60 minutes. 5 L of oligosaccharide extract was obtained.

Example 2. Preparation of Kelp Beverage

A kelp drink was prepared by adding a predetermined ratio of the beverage additives of the ingredients shown in Table 1 to the kelp polysaccharide and oligosaccharide extracts obtained in Example 1.

Material and Additives content (%) Kelp polysaccharide and oligosaccharide extract of Example 1 100 Chondroitin Sulfate 0.5 glucose 4 honey One Apple Extract Concentrate (50 Brix) 4 Angelica Extract One Vitamin c 0.001

Experimental Example 1. The effect of kelp polysaccharide and oligosaccharide extract on blood lipid and cholesterol reduction

1-1. Laboratory Animals and Treatment

For experimental animals, received from the Korea Experimental Animal Center for 1 week in the animal breeding room under constant conditions (temperature: 22 ± 1 ℃, humidity: 55 ± 3%, contrast: 12 hours light / dark cycle). Sprague-Dawley male rats and ICR mice (20 ± 2 g) weighing 200 ± 10 g body weight were used.

Induction of dietary hyperlipidemia in experimental animals was induced by hypercholesterolemia for 6 weeks with a cholesterol diet supplemented with 1% cholesterol and 0.5% Na-cholic acid.

As a sample, 10 ml / kg of the kelp polysaccharide and oligosaccharide extract of Example 1 and the kelp drink of Example 2 were administered, and as a reference, cholestyramine resin (CSR, 100 mg / kg, Lucky Pharmaceutical) Was suspended in physiological saline and administered orally once a day using a needle zonde each for the last two weeks of hyperlipidemia induction.

1-2. Changes in Body Weight and Adipose Tissue in Laboratory Animals

The weight change of the experimental animals was measured every week from the start of the experiment to calculate the weight change for the initial weight, the adipose tissue was calculated by taking the fat around the testicles.

The results of observing the effects of the kelp polysaccharide and oligosaccharide extract of Example 1 and the kelp drink of Example 2 on the weight and weight to organ weight ratio in rats induced dietary hyperlipidemia are shown in Table 2 below. Induction group of hyperlipidemia was 245.9 ± 8.91 (g) at 6 weeks of breeding, and it was found that body weight was significantly increased compared to normal group 163.7 ± 7.89 (g).

However, it was confirmed that the weight gain was significantly suppressed to 184.3 ± 6.98 (g), especially when the kelp polysaccharide and oligosaccharide extract of Example 1 was administered, which was higher than the control group cholestyramine resin (190.8 ± 7.10 ^ef ).

In addition, the weight gain of adipose tissue (39.8 ± 2.34 g), which was increased in rats inducing dietary hyperlipidemia, was also significantly improved (26.4 ± 2.11 g) by administration of the kelp drink of Example 2 above.

group Intake (mg / kg) Weight (6 weeks) Adipose tissue (testes, mg / body wt.) Normal diet. 163.7 ± 7.89 ^h 18.0 ± 1.42 ⁱ Hyperlipidemia induction group 245.9 ± 8.91 ^bc 39.8 ± 2.34 ^bc Kelp polysaccharide and oligosaccharide extract of Example 1 10 184.3 ± 6.98 ^fg 30.1 ± 3.24 ^efg Kelp drink of Example 2 10 198.2 ± 8.77 ^de 26.4 ± 2.11 ^hi CSR (control) 100 190.8 ± 7.10 ^ef 25.1 ± 1.87 ^hi   * Each sample and CSR (cholestyramine resin) were administered orally daily for 2 weeks, and rats were sacrificed 24 hours after the final salary, with mean ± S.D. (N = 8). 1) The numerical value represents the mean ± S.D. (n = 5). 2) Numbers marked with the same initials were not significantly different from each other by Duncan's multi-range test (P <0.05)

1-3. Determination of Total Lipid, Phospholipid, and Neutral Lipid Contents in Laboratory Animals

1-3-1. Determination of total lipid content in blood

Total lipid content was determined using 10 ml of sample and cH ₂ SO ₄ 2 according to the method of Frings et al. (Frings CS. Et al., Am. J. Clin. Path. , 53 , pp89-92, 1970). After 10 ml of the solution was kept in a 95 ° C. water bath, 0.1 ml of the mixed solution was added, phospho-vanillin reagent was added thereto, and then incubated at 37 ° C. for 15 minutes to absorb the reagent blank at a wavelength of 540 nm. Was measured.

In the rats induced dietary hyperlipidemia, the effects of the kelp polysaccharide and oligosaccharide extract of Example 1 and the blood total lipid content of the kelp beverage of Example 2 were tested. Serum total lipid content of the normal diet group was 232.2 ± 12.2 mg / ,, but significantly increased to 381.4 ± 15.3 mg / 에서는 in hyperlipidemic rats.

However, in the diet fed with the kelp polysaccharide and oligosaccharide extract of Example 1 and the kelp drink of Example 2, the lipid content of the mice with anemia was reduced by about 15-20% (p <0.05). It was confirmed that.

1-3-2. Determination of blood phospholipid content

Phospholipid content was measured using a kit (Iatron Chem., Co.) prepared according to the enzyme method of Chen et al. (Chen PS, Anal. Chem. , 28 , pp 1756-1790, 1956). That is, the enzyme reagent [containing phospholipase 3.9 U, choline oxidase 5.6 U, peroxidase 3.6 U, 4-aminoantipyrine (0.3252 mg)] After dissolving in enzyme reagent solution [containing tris (hydroxy methyl) -aminomethane 6.057 mg], add 3.0 ml of the prepared enzyme solution to 20 µl of the sample, and incubate for 20 minutes at 37 ° C. The absorbance was measured at a wavelength of 500 nm as a control for the blank. The content is expressed in mg / dl according to the standard weight loss line.

In the rats induced dietary hyperlipidemia, the effects of the kelp polysaccharide and oligosaccharide extract of Example 1 and the blood phospholipids of the kelp beverage of Example 2 were tested. Phospholipid content in the blood did not show a significant difference between the hyperlipidemia induced group, the kelp polysaccharide and oligosaccharide extract of Example 1, and the kelp beverage administration group of Example 2.

1-3-3. Determination of blood triglyceride content

Triglyceride content was measured using a kit (AM 157S-K, Asan) prepared according to McGowan et al . (McGowan MW., Clin, Chem , 29 , pp538-543, 1983). That is, enzyme reagent [lipoprotein lipase (lipoprotein lipase) 10800 U, glycerol kinase 5.4 U, peroxidase 135000 U, L-α-glycerophosphooxidase (L-α) -U-glycerophosphooxidase)] was dissolved in an enzyme reagent solution [containing 0.427 g / dl of N, N-bis (2-hydroxyethyl) -2-aminomethanesulfonic acid], and then 3.0 ml of the enzyme solution prepared in 20 ml of the sample. After the addition was incubated for 10 minutes at 37 ℃ the reagent blank was measured for absorbance at a wavelength of 550 nm as a control.

In the rats induced dietary hyperlipidemia, the effects of the kelp polysaccharide and oligosaccharide extract of Example 1 and the concentration of triglycerides in the blood of the kelp beverage of Example 2 are shown in Table 3 below. The triglyceride content was 86.3 ± 8.21 mg / dl in the normal diet group and 195.0 ± 7.36 mg / dl in the hyperlipidemia induction group. On the other hand, the administration of the kelp polysaccharide and oligosaccharide extract of Example 1 and the kelp beverage of Example 2 reduced the content of neutral lipids by 17% (p <0.05) and 30% (p <0.05), respectively. The decrease in triglyceride content of kelp beverages was higher in the control group compared to cholestyramine resin.

group Intake (mg / kg) Total lipid (mg / mg) Phospholipids (mg / dl) Neutral Lipid (mg / ㎗) Normal diet. 232.2 ± 12.2 ^e 119.1 ± 14.7 ^ns 86.3 ± 8.21 ^e Hyperlipidemia induction group 381.3 ± 15.3 ^a 130.6 ± 17.4 195.0 ± 7.36 ^a Kelp polysaccharide and oligosaccharide extract of Example 1 10 320.1 ± 23.6 ^bcd 128.7 ± 10.5 161.7 ± 6.77 ^bc Kojima drink of Example 2 10 310.4 ± 18.2 ^bcd 131.7 ± 10.6 140.7 ± 6.44 ^cd CSR (control) 100 300.1 ± 14.9 ^cd 135.6 ± 15.5 150.6 ± 4.89 ^c  1) The numerical value represents the mean ± S.D. (n = 8). 2) The same initial numbers were not significantly different from each other by Duncan's multi-range test (P <0.05).

As a result, it was found that the kelp polysaccharide and oligosaccharide extract obtained by the acid and high temperature heating treatment and the kelp beverage containing the same as an active ingredient were effective in reducing serum lipids, in particular, it was effective in inhibiting the increase of neutral lipid. This is meaningful in terms of reducing the factors causing hyperlipidemia and atherosclerosis caused by the accumulation of triglycerides.

1-4. Determination of Total Cholesterol, Neutral Lipid, High Density Lipoprotein (HDL), Low Density Lipoprotein (LDL) and Arteriosclerosis Index in Experimental Animals

1-4-1. Determination of Total Cholesterol Content in Laboratory Animals

The determination of total cholesterol content was carried out using kits prepared by Richmond's enzyme method (Richmond W., Clin, Chem , 22 , pp1579-1584, 1976) (AM 202-K, Asan). In other words, enzyme reagent (containing 20.5 U / L of cholesterol esterase, 10.7 U / L of cholesterol oxidase, 1.81 g / L of sodium hydroxide) in an ice cold phase After dissolving in a lysate (containing 13.6 g / l of potassium phosphate monobasic and 1.88 g / l of phenol), 3.0 ml of the enzyme solution prepared in 20 µl of the sample was added and incubated at 37 ° C for 5 minutes. The absorbance was measured at a wavelength of 500 nm with the reagent blank as a control. According to the standard calibration curve, the blood content was expressed in mg / dl.

Table 4 shows the results of examining the effect on the serum total cholesterol content of the kelp polysaccharide and oligosaccharide extract of Example 1 and the kelp beverage of Example 2 in the rat induced dietary hyperlipidemia.

The total cholesterol content of the blood was 173.4 ± 6.88 mg / dL due to hyperlipidemia induction of 142.1 ± 3.82 mg / dL of the polysaccharide and oligosaccharide extract of Example 1, 127.4 by the administration of the kelp beverage of Example 2 It can be seen that the lowering to ± 4.99 mg / ㎗.

1-4-2. Measurement of High Density Lipoprotein (HDL) and Low Density Lipoprotein (LDL) Content in Laboratory Animals

The measurement of high density lipoprotein (HDL-cholesterol) content and low density lipoprotein (LDL-cholesterol) was performed by enzyme method such as Noma A. et al., Clin. Chem. , 24 , pp1504-1511, 1978. Experiment was carried out using the prepared kit (AM 203-K, Asan). In other words, add 0.2 ml of sedimentation reagent (containing 0.1% dextran sulfate and 0.1 M magnesium chloride) to 20 µl of serum, mix well, and let stand for 10 minutes at room temperature and centrifuge at 3000 rpm for 10 minutes. Then, 01 ml of the supernatant was taken, mixed well with 3.0 ml of enzyme solution, incubated at 37 ° C. for 5 minutes, and the absorbance was measured at a wavelength of 500 nm as a control blank. According to the standard calibration curve, the content is expressed in mg / dl.

The results of investigating the effects of the kelp polysaccharide and oligosaccharide extract of Example 1, serum high density lipoprotein (HDL) and low density lipoprotein (LDL) content of the kelp beverage of Example 2 in rats induced dietary hyperlipidemia Indicated.

The HDL-cholesterol level was not significantly different from that of the hyperlipidemic induction group, the kelp polysaccharide and oligosaccharide extract of Example 1, and the kelp drink of Example 2. LDL-cholesterol was 112.4 ± 9.11 mg / dL in hyperlipidemic rats, but significantly decreased to 70.9 ± 5.44 ^def when the polysaccharide and oligosaccharide extracts of Example 1 were administered, which was 70.3 ± 5.66 ^def when the control group cholestyramine resin was administered. Similar to Even when administering the kelp drink of Example 2, it was significantly lower than the hyperlipidemia induction group with 73.2 ± 8.12 ^def .

1-4-3. Measurement of Arteriosclerosis Index in Experimental Animals

As a result of measuring the arteriosclerosis index (AI) of the experimental animals, 5.47 of the rats of the hyperlipidemia inducing group were 3.85 ± 0.21 when the polysaccharide and oligosaccharide extracts of Example 1 were administered and 4.26 when the kelp beverage of Example 2 was administered. It can be seen that the lowered to ± 0.30. This is lower than the CSR administration group, but it indicates that the effect of lowering the arteriosclerosis index by administering the kelp extract of the present invention.

group Intake (mg / kg) Total Cholesterol (mg / ㎗) HDL-cholesterol (mg / dl) LDL-cholesterol (mg / dl) Arteriosclerosis index Normal diet. 91.2 ± 7.07 ^g 39.7 ± 1.15 ^a 60.3 ± 4.10 ^f 1.30 ± 0.09 ^f Hyperlipidemia induction group (A) 173.4 ± 6.88 ^a 26.8 ± 1.86 ^bcde 112.4 ± 9.11 ^a 5.47 ± 0.33 ^a Kelp polysaccharide and oligosaccharide extract of Example 1 10 142.1 ± 3.82 ^cd 29.3 ± 3.04 ^bc 70.9 ± 5.44 ^def 3.85 ± 0.21 ^c Kelp drink of Example 2 10 127.4 ± 4.99 ^e 24.2 ± 2.09 ^de 73.2 ± 8.12 ^def 4.26 ± 0.30 ^bc CSR 100 109.7 ± 4.00 ^f 29.9 ± 1.13 ^b 70.3 ± 5.66 ^def 2.67 ± 0.25 ^e  1) The numerical value represents the mean ± S.D. (n = 8). 2) The same initial numbers were not significantly different from each other by Duncan's multi-range test (P <0.05). 3) AI = [total cholesterol- (HDL-cholesterol) / (HDL-cholesterol)]

Hyperlipidemia, on the other hand, is an index of arteriosclerosis, which increases the synthesis of triglycerides and secretion of chylomicrons in the small intestine, the synthesis of triglycerides in the liver, the synthesis and secretion of VLDL and LDL-C, and the synthesis of HDL-C This is due to decreased elimination of triglycerides in peripheral tissues due to decreased lipase activity (Goldstein et al., Am. J. Med ., 58, p147-151, 1975; Ross R., New.Eng J. Med ., 314, p488-495, 1986).

In rats induced by dietary hyperlipidemia in this experiment, the LDL-cholesterol content was recovered to almost normal levels by the treatment of polysaccharide and oligosaccharide extract of Example 1, and the blood cholesterol content of Example 2 was significantly increased. The treatment of kelp beverages was reduced. Therefore, it can be seen that the kelp polysaccharide and oligosaccharide extract of the present invention can be developed as a functional food composition, particularly a functional health drink, as a functional food composition for lowering serum lipids and cholesterol.

Examples of the formulation of the composition are described below, but are not intended to limit the present invention but to explain in detail only.

Formulation Example 1 Preparation of Healthy Food

Kelp polysaccharide and oligosaccharide extract of Example 1 ... 1000 mg

Vitamin Mixture ......................

Vitamin A Acetate ............... 70 μg

Vitamin E ................................. 1.0 mg

Vitamin B1 ..................... 0.13 mg

Vitamin B2 ..................... 0.15 mg

Vitamin B6 ......................................... 0.5 mg

Vitamin B12 .................. 0.2 μg

Vitamin C ......................................... 10 mg

Biotin ......................................... 10 μg

Nicotinamide ... 1.7 mg

Folic acid ......................................... 50 ㎍

Calcium Pantothenate ......................................... 0.5 mg

Inorganic mixtures ...............

Ferrous Sulfate ............... 1.75 mg

Zinc Oxide ......................................... 0.82 mg

Magnesium Carbonate ......................................... 25.3 mg

Potassium monophosphate ......................................... 15 mg

Dibasic calcium phosphate ........................ 55 mg

Potassium Citrate ..... 90 mg

Calcium Carbonate ... 100 mg

Magnesium Chloride ............... 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixtures is mixed with a component suitable for a health food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method. The granules may be prepared and used for preparing a health food composition according to a conventional method.

Formulation Example 2 Preparation of Healthy Drinks

Kelp polysaccharide and oligosaccharide extract of Example 1 .. 1000 mg

Citric Acid ..................................... 1000 mg

Oligosaccharide ......................................... 100 g

Plum concentrate ........................... 2 g

Taurine ......................................... 1 g

Purified water is added ............... 900 ml total

After mixing the above components in accordance with the conventional healthy beverage manufacturing method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and refrigerated Used to prepare the healthy beverage composition of the invention.

Although the composition ratio is mixed with a relatively suitable component for a preferred beverage in a preferred embodiment, the compounding ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and usage.

As described above, the kelp polysaccharide and oligosaccharide extract obtained by the acid and high temperature heating treatment of the present invention has an effect of lowering serum lipid and cholesterol content, and as a functional food composition for lowering serum lipid and cholesterol, the health of functional health drinks and the like. Can be used for nutraceuticals.

Claims (8)

Functional food composition for lowering serum lipids and cholesterol, containing kelp polysaccharide and oligosaccharide extract obtained by acid and high temperature heating as an active ingredient. The functional food composition of claim 1, wherein the kelp is L. japonica . The functional food composition according to claim 1, wherein the acid is treated with citric acid. The functional food composition of claim 1, wherein the high temperature heating is performed at 100 to 150 ° C. for 10 minutes to 5 hours. The functional food composition of claim 1, wherein the kelp polysaccharide and oligosaccharide are mainly alginic acid oligosaccharides. The functional food composition of claim 1, comprising 0.02 to 90% by weight of said extract relative to the total weight of the composition. A dietary supplement comprising the kelp polysaccharide and oligosaccharide extract of claim 1, which exhibits serum lipid and cholesterol lowering effects, and a food supplement acceptable food supplement. The health functional food according to claim 7, which is a health beverage.