KR20080098193A - Saengmaeksan extract and saengmaeksan bean curd using saengmaeksan extract - Google Patents

Abstract

Saengmaeksan(Oriental medicine therapy) extracts is provided to reduce the blood alcohol concentration and The lipid concentration of the neutral and blood cholesterol by being extracted from snake's beard, Maximowiczia typical, ginseng, pumpkin and hedysarum. A bean curd by using Saengmaeksan(Oriental medicine therapy) extracts comprises the following steps. Snake's beard 2.6-5.0%, Maximowiczia typical 2.6-5.0%, ginseng 1.0-5.0%,, pumpkin 2.6-5.0% and hedysarum 2.6-5.0% are mixed as the rate of weight% and Concentrate is extracted by heating at 95-130deg.C for 2-5 hours in the pot for preparing medicine and filters to the gauze and centrifuges at the speed of 5000~8000 X g for 10-30min. to make Saengmaeksan(Oriental medicine therapy) extracts. soy oil 95-97%(v/v) consisting of bean is mixed with the Saengmaeksan(Oriental medicine therapy) extracts3 ~ 5%(v/v).

Description

Saengmaeksan extract and saengmaeksan bean curd using saengmaeksan extract}

Figure 1 shows the results of the average weight increase rate measured the amount of growth and weight change for 4 weeks after the administration of the sample of the animal Sprague-Dawley (male).

Figure 2 shows the results of the total weight change measured the growth and weight change for 4 weeks after the administration of the sample of the animal Sprague-Dawley (male).

Figure 3 shows the lactic acid hydrolysis effect of the raw mach acid extract in the body.

Figure 4 shows the alcohol degradation effect of the body extract of draft malt.

Figure 5 shows the triglyceride content in plasma.

Figure 6 shows the cholesterol content in plasma.

Figure 7 shows the change in plasma AST levels after administration of the sample for 4 weeks to determine the hepatoprotective effect of the general tofu administration group (T1), live maize tofu administration group (T2), and the raw maw extract extract group (T3).

Figure 8 shows the change in plasma ALT levels after administration of the sample for 4 weeks to determine the hepatoprotective effect of the normal tofu administration group (T1), live maize tofu administration group (T2) and the raw maize extract administration group (T3).

Figure 9 shows the antioxidant effect of the draft tofu.

In the present invention, by weight ratio of 2.5 to 5.0%, Schisandra chinensis 2.6 to 5.0%, Astragalus 2.6 to 5.0%, Ginseng 1.0 to 5.0%, Pumpkin 2.6 to 5.0% and distilled water 75 to 87% by mixing for 2 to 5 hours Extracted from the concentrated solution by heating in a bath at a temperature of 95 to 130 ℃, filtered with gauze, centrifuged in a centrifuge at a rate of 5000 to 8000 × g for 10 to 30 minutes to extract the raw malic acid extract will be.

In addition, respectively, by weight ratio 2.6 to 5.0%, Schisandra chinensis 2.6 to 5.0%, Astragalus 2.6 to 5.0%, Ginseng 1.0 to 5.0%, pumpkin 2.6 to 5.0% and distilled water 75 to 87% for 2 to 5 hours The concentrated liquid was extracted by heating in a shaker at a temperature of 95 to 130 ° C., filtered with gauze, and centrifuged at a speed of 5000 to 8000 × g for 10 to 30 minutes to extract raw maize extract, followed by 10% of soybeans. (v / v) The present invention relates to the raw machinate extract and tofu using the extract, characterized in that it is prepared by mixing the 3 to 5% (v / v) of the raw malic acid extract in soymilk 95-97% (v / v). .

The extract of the present invention and the herbal medicine used in the raw tofu mountain using the extract is a medicine known as the raw malic acid, blood, blood donation in the traditional medicine, including Dongbogam, blood, and boredom 인한 (음 無力) It is recorded as a typical prescription that has therapeutic functions such as dyspnea, dizziness, sea water, and heat sensation. Its constituents are Megmun-dong, Schisandra chinensis, Ginseng and Astragalus. According to the internal and external wound plate theory, the raw malt mixed with the above-mentioned herbs is composed of 12g of Macmundong and 6g of ginseng and 5g of Schisandra chinensis. 사용되고) It is used for boredom, respiratory distress, dizziness, seawater, heat sensation due to lung tone, and its effect is ripe and cold. ) Has the effect of. The chief physician is weak in his clan, or clumsy, in construction, due to lack of cough. Symptoms such as dysphagia and self-injury, which are caused by coughing due to a symptom and dysfunction due to injury. Cure). In the present invention, the extract was prepared by adding zucchini to the draft malt, and this was used as a tofu coagulant to prepare the draft tofu.

The medicinal herb extract of the present invention and the medicinal herbs including McMun-Dong, which is the main component of the raw MIC extract used as a tofu coagulant in tofu coagulant tofu, were purchased from Kyungdong Market. Same as Macmun-dong belongs to the family Liliaceae, and its scientific name is Ophiopogon japonicus KER-GAWLER. It contains mucus, small amounts of β-sitosterol and vitamin A-like substances, and is said to be yunjosaeng (화 生 津) and haematjihaeji (化痰止咳). Its origin is dried roots of lichen plant Jigyecho, its efficacy is Yin-Yum Yun-Jung, Saengjin-Jeong-Jeong, and the chief physician is pulmonary condition, soil, blood and hemoptysis. (喀 血), lung (肺), lung 肺 癰 (열 口), heat-drying structure (허 口燥), it is used for gynecological heat, etc. In pharmacological experiments strong cardiac effect, diuretic effect, sputum cutting effect, cough Whining, nutrition, fungi has been found.

Schizandra chinensis BALL. (Schisandra chinensis BALL.) Is a dried fruit of mature fruit, schizandrin, vitamin A-shaped substance. , Vitamin C, organic acids, ether extracts (抽出 物) has been recognized various pharmacology (藥理). Pneumonia Self-Contained, Raw Pulmonary Dome, Insertion Branch, Central Nervous System Excited, Jinhaedam, Uterine Excited, Antibacterial ), Transaminase lowering action (降下 作用) is recognized and used in lung Xinyangheo (肺 腎陽虛). This product is equipped with Omi, especially since it is excellent in acid and rice, it collects and discards used wastes and ripens.益腎 氣) to confound the circle (진 元). It is warm in nature but not dry, so it can be used regardless of yin huo (폐) and Yang (() of the lung. In general, it is used to treat shallowing due to ruins, self-limiting, self-limiting, limiting, bone tingling caused by new license, and inability to speak. In pharmacological experiments, central nervous system excitement, fatigue recovery, cardiovascular function recovery, blood pressure control, gastric juice secretion, blood glucose lowering, glycogen-promoting, etc. were found.

Panax ginseng is a dried product of the root of Panax ginseng GAMEY. Ginseng Panacene, panaquilon, panaxin, and ginseng. Ginseng, Ginseng, Vitamin A, B1, B2, C, Sucrose, Glucose, Fructose, Malt Sugar, Choine, Inorganic Salt, Glutinous, Mucus Daebowongi, Anshin-ikji, Geunbi-ikgi, Geunjin-jin, nervous system excitement, adrenal cortex excitement Mechanism, gonadotropin-like action, cardiac, hypoglycemic, digestive absorption and metabolic hyperactivity, antidiuresis, anaphylaxis, etc. It is mainly used for acute shock and chronic weakness.

Astragalus belongs to the legumes and is the dried product of the root of Astragalus membranaceus BUNGE. (Hwanggi), which contains hydrochloric acid, corin, amino acids, etc. ) This product is known as a summary to treat vain, and when used in the case of vain embarrassment, it can be seen as an elevation, and in the case of a sign blotting. If you use it, it is highly labeled. (부 表 止汗), if you use it for deficiency of Gibgi. If you use it for gastrointestinal bleeding (氣虛 血 痺), it is a hot hardening blood (溫 經 和 血). Horo is a fruit of Lagenaria siceraria (MOLINA.) STANDL. (Chestnut flower), which contains glucose, pentosan, etc., and dihydrate, antipyretic, hygroscopic species. 사용) used in pharmacological experiments, tonic action, immune function control action, cardiac action, diuretic action, blood pressure lowering action, anti-inflammatory action was found.

Zucchini belongs to the family of cucurbits, scientific name Cucurbita spp, and according to the synonym of the pumpkin, it is recorded that the taste is sweet, and it has the effect of relaxing five chapters and brightening eyes. And the well-known benefits of swelling after giving birth, diabetes and insomnia are effective. The older the pumpkin, the sweeter the sugar increases as it ripens. But the sugar that pumpkin has is good for digestion and absorption, so it is very good for people with weak stomachs or patients with recovery periods. Adult disease, edema, diarrhea, cough, cold, night blindness, and stroke It is good for prevention, carotene contained in the pumpkin shows the effect of vitamin A when it enters the body, vitamin A plays a role in strengthening the mucous membrane to increase the resistance to colds. In addition, it is rich in minerals, vitamin B and vitamin C. It is good for women with coldness because it improves metabolism and immunity and warms the body. Yellow vegetables contain antioxidant beta-carotene, lung cancer, esophageal cancer, stomach cancer and bladder cancer. , Laryngeal cancer, prostate cancer, etc. It is effective in inhibiting intestinal viruses and carcinogens because it contains a large amount of this ingredient in old pumpkin.

The present invention may be produced by extracting a mixture of medicinal herbs such as Macmundong, Schisandra chinensis, ginseng, Astragalus, pumpkin, etc., the raw malt extract may exhibit anti-fatigue effect as lactic acid hydrolase, and not only has an alcohol-degrading effect that significantly reduces blood alcohol concentration. The tofu coagulated with raw malic acid extract lowers the concentrations of blood cholesterol and triglycerides, which are the main factors inducing cardiovascular diseases such as hypertension or arteriosclerosis. It can be seen that the antioxidant activity to prevent the increase is significantly higher than when administered the raw malic acid extract alone, and many researches and efforts are underway in the development of drugs and functional substances that can exhibit such effects, especially in the human body The above medicine, or functional water from a few natural products To develop and to the anti-fatigue effect of the new and alcoholysis effect and anti-hyperlipemia effect and the extract saengmaeksan saengmaeksan head provided with the extract to develop a liver function activity and anti-oxidant for that purpose.

The present invention for achieving the above object is by weight ratio of pulsatile 2.6 to 5.0%, Schisandra 2.6 to 5.0%, Astragalus 2.6 to 5.0%, ginseng 1.0 to 5.0%, pumpkin 2.6 to 5.0% and distilled water 75 to 87% of the mixture was heated in a shaker at a temperature of 95 to 130 ° C. for 2 to 5 hours to extract the concentrate, filtered with gauze, and centrifuged at a speed of 5000 to 8000 × g for 10 to 30 minutes to extract the extract. It provides a raw malic acid extract.

In addition, respectively, by weight ratio 2.6 to 5.0%, Schisandra chinensis 2.6 to 5.0%, Astragalus 2.6 to 5.0%, Ginseng 1.0 to 5.0%, pumpkin 2.6 to 5.0% and distilled water 75 to 87% for 2 to 5 hours The concentrated liquid was extracted by heating in a shaker at a temperature of 95 to 130 ° C., filtered with gauze, and centrifuged at a speed of 5000 to 8000 × g for 10 to 30 minutes to extract raw maize extract, followed by 10% of soybeans. (v / v) Soymilk 95-97% (v / v) to provide the raw maengfu tofu using the raw machinate extract prepared by mixing the 3 ~ 5% (v / v) the raw machin extract.

The raw malt extract of the present invention is mixed with McMun-Dong, Schisandra chinensis, Astragalus, Ginseng and Zucchini, and then added 1500 mL of distilled water to a total of 300 g and extracted in a medicinal herb (Daewoong medicinal herb) for 3 hours, followed by primary filtration using gauze and at 8,000 × g. Centrifugation for 15 minutes was provided with raw malic acid extract.

In addition, Shangmasan tofu using the raw malic acid extract is mixed with Mengmundong, Schisandra chinensis, Astragalus, ginseng and pumpkin, and added to the total 300g by adding 1500mL of distilled water, and heated in a bath at a temperature of 110 ℃ for 3 hours to extract the concentrate, Filtration with gauze, centrifuge at 8,000 × g for 15 minutes to extract the raw machi extract by centrifugation, the extracted machi extract was stored at 4 ℃ prepared as a tofu coagulant, 10% known (v / v 4) Tofu was prepared by adding 4% (v / v) of the said raw malic acid extract as a tofu coagulant and adding 2% (v / v) of 20% magnesium chloride solution as a positive control.

In order to prepare the raw tofu used in the present invention, after preparing a conventional 10% (v / v) soymilk solution, the sample was cut for 40 minutes while stirring well. At this time, if bubbles were generated, 4-5 drops of soybean oil were added. The cut soy milk solution was filtered using gauze to separate soybean milk and curd refuse. After cooling the separated soymilk to 85 ~ 90 ℃ 50mL soymilk was dispensed into a beaker. As the tofu coagulant, 3 ~ 5% (v / v) of the raw malic acid extract was added to the beaker and mixed, 2% (v / v) of 20% MgCl ₂ solution was added as a positive control group, and distilled water was added as a negative control group. . Tofu was prepared by leaving the sample containing the tofu coagulant at room temperature for 1 hour.

Experimental Example 1: Classification of experimental animals and experimental groups

The experimental animals were purchased from the experimental animal center of Sprague Dawley species of 100 ± 10g and 4 weeks old male rats, and adapted for 4 weeks in the animal breeding room of Konkuk University. During the four weeks of adaptation, the temperature and humidity of the cage were maintained at 22 ± 2 ° C and 55 ± 5%, respectively. The diet was intended to consume solid feed (Samyang Corporation) and water freely.

Seven rats weighing 350 g (8 weeks of age) adapted for 4 weeks were divided into normal control, negative control, positive control, and test groups. . The negative control group consumed alcohol and water, and the positive control group consumed J's hangover relievers (hangover and liver function recovery) and alcohol.

The experimental group included the T1 group (hereinafter referred to as T1) which administered alcohol and commercial tofu of P company (hereinafter referred to as T1), the T2 group (hereinafter referred to as T2) which used alcohol and drafted tofu, and the extract of alcohol and vegetated acid (Macmundong + ginseng + Schisandra + Astragalus + amber) was set to the T3 group (hereinafter referred to as T3) administered. At this time, the diet was freely supplied with solid feed (Samyang Corporation), and alcohol was calculated to consume 5g / kg.bw / day per rat, and was supplied diluted in drinking water daily. The treatment of each group is shown in Table 1.

Table 1. Composition of Groups

Group No. of exam Treatment  No.1 (Normal Control) 7 None-alcohol  No.2 (Negative control) 7 Alcohol + Water  No.3 (Positive Control) 7 Alcohol + Hangover Relief  No.4 (Test group1-T1) 7 Alcohol + regular tofu  No.5 (Test group2-T2) 7 Alcohol + draft tofu  No.6 (Test group3-T3) 7 Alcohol + Raw Wheat Extract *

* Salt extract: Limoope platyphylla + Schizandra chinensis + Ginseng (Panax ginseng C.A. Meyer) + Astragalus membranaceus BUNGE + Pumpkin (Cucurbita spp.)

Experimental Example 2: Preparation and Administration of Sample

The raw malt extract was made by mixing Mengmun-dong, Schisandra chinensis, Astragalus, Ginseng and Zucchini and adding extracted distilled water to heat extract. The general tofu was purchased commercially, and the tofu malted tofu was 3 ~ Tofu was coagulated directly in the laboratory with 5% raw malate extract.

Normal tofu and raw mackerel tofu were calculated for 350g rats based on the intake of 250g of tofu by 60kg adults, and the raw maize extract was also suitable for 350g rats based on the intake of 100g of herbal medicine per day for 60kg adults. The amount was calculated and orally administered.

On the last day of breeding, the animals are fasted overnight for 16 hours, and the abdomen is incised with weakly anesthetized with ethyl ether. Placed in EDTA tube to prevent blood clotting. The collected blood was used for Lactate dehydrogenase (LDH) analysis, blood alcohol concentration measurement, Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), cholesterol, triglyceride (TG) and Malone dialdehyde (MDA) analysis. Liver and kidneys were extracted immediately after blood collection, immediately washed with cooled saline, dried on filter paper, and weighed.

Body weights were measured at the same time once a week for four weeks from the day of drug administration to the day before necropsy. After the autopsy, the weight of the organ was collected by finding the posterior vena cava of the experimental animal, and then the liver and kidney were extracted and the weight thereof was measured.

Experimental Example 3: Growth, weight change and organ weight change

Pirola and Lieber et al., Piola RC and Lieber CS. Energy wastage in rats given drugs that induce microsomalenzymes. J Nutr 105: 1544-1548, 1975 reported that weight gain was reduced when alcohol was ingested in rats. Another study by Piola RC and Lieber CS. The energy cost of the metabolism of drugs, including ethanol, Phamacology 7: 185, 1972, suggests that when people consume 50% of their energy in liquor instead of sugar, they lose weight. This is because alcohol consumption increases oxygen consumption, increases metabolic rate, and decreases ATP production of alcohol oxidative machinery in intracellular microsomes. Gruchow HW, Sobocinski KA, Barboriak JJ and Scheller JG. Alcohol consumption, nutrient intake and relative body weight among U.S. adults. Am J Clin Nutr 42: 289-595, 1985].

The results of the present invention also showed a similar trend, as measured in the growth and weight change of 4 weeks after the administration of the sample Sprague-Dawley (male) of the experimental animals as shown in Table 2, Figure 1 and 2. In other words, the alcohol-treated negative control group showed significantly lower body weight change than the other groups, and the T1 group administered normal tofu, the T2 group treated with green maize tofu and the T3 group treated with green maize extract were significantly lower than the negative control group. Body weight showed a significant increase close to the normal control group, especially in the T2 group treated with draft maize tofu. On the other hand, the T1 group who received normal tofu showed a similar weight change as the negative control group, indicating that the weight loss due to alcohol consumption was hardly recovered.

Experimental Example 4: Weight of Organs

Long-term intake of ethanol causes the liver to become enlarged by fibrosis of fatty liver and liver. Han, B. H., M.H. Park, L.K.Woo and Y.N. Han. (1979) Studies on the anti-oxidant components of Korea Ginseng, Korea Biochem. J. , 12:33] The weight of liver was increased in all groups ingested alcohol by the accumulation of lipids in liver tissue by alcohol intake [Levy, R.I., Bonnel, M. and Ernst, N.D. (1976) J. Am. Dieter. Assoc. 58: 406-417. And Lieber, C. S., Don P. Jones. and M. DeCarli. (1965) Am. J. Clin. Nutr., 44 (6): 1009] and the experimental results showed similar trends. In the results shown in Table 2, the negative control group with long-term administration of alcohol significantly increased the replacement intermediate ratio (% of liver weight per 100g body weight of the test animal) compared to the normal control group without alcohol. Therefore, long-term administration of alcohol is consistent with the above report. On the other hand, T2 and T3 groups treated with raw maize tofu or extract of maeng acid showed a recovery rate near the normal control group, which was effective in treating and improving fibrosis of fatty liver and liver due to prolonged alcohol administration. The rate of replacement neutrophil equipment (% of kidney weight per 100g body weight of the experimental animals) showed that all experimental groups showed a significant (p <0.5) level in the negative control group and were close to the normal control group. Indicated. In particular, the T2 and T3 groups showed a recovery effect, and the positive control group and the T1 group also showed less effective values than the T2 and T3 groups.

Table 2.Total body weight gains and the weight ratio of liver and kidney

 Group Total body weight gains (g) Mean ± S.D. Liver (% of body weight) Mean ± S.D. Kidney (% of body weight) Mean ± S.D. No.1 (None-alcohol) 43.67 ± 5.96 2.646 ± 0.05 0.619 ± 0.02 No.2 (Alcohol) 35.17 ± 5.98 ^** 2.873 ± 0.07 ^*** 0.652 ± 0.03 ^* No.3 (alcohol + hangover remover) 36.00 ± 3.78 2.705 ± 0.06 0.627 ± 003 ^* No.4 (T1) 36.00 ± 7.37 2.716 ± 0.33 0.628 ± 0.04 ^* No.5 (T2) 43.14 ± 10.11 ^* 2.620 ± 0.12 ^** 0.615 ± 0.03 ^* No.6 (T3) 41.57 ± 11.37 ^* 2.624 ± 0.13 ^** 0.619 ± 0.04 ^*

(Each value represents the mean ± S.D. Of 7 rats)

Means with different superscript asterisks within a column and significantly different from each other at P <0.5 (*) and P <0.05 (**) and P <0.005 (***) as determined by Student's T-test.

Experimental Example 5: Biochemical Test (LDH, Alcohol Concentration, AST, ALT, Cholesterol, TG, MDA)

LDH activity was measured using an automated biochemistry analyzer (Hitachi747, Japan) using an LDH kit (Boehringer Mannheim, Germany). After fasting the rats for 16 to 18 hours before the experiment, the first dose was administered with 2 ml of test substance + 20% alcohol, and after 1 hour, 2 ml of 20% alcohol was administered for 1 hour after 2 hours. After the third dose, 20ml alcohol + 2 ml of test substance was administered, and 3 hours after the last test, blood was collected and allowed to stand at room temperature for 30 minutes, followed by centrifugation (3000xg.15min). Serum was isolated and analyzed for the lactic acid accumulated in the body, which is known to be highly related to systemic fatigue.

Ethanol concentration was measured using a ethanol kit (Roche, swizerland) using a biochemical analyzer (Cobas Integra, Roche, swizerland). After fasting the rats for 16 to 18 hours before the experiment, the first dose was administered with 2 ml of test substance + 20% alcohol, and after 1 hour, 2 ml of 20% alcohol was administered for 2 hours. After the third dose, 20 ml alcohol + 2 ml of test substance was administered, and after 90 minutes and 180 minutes after the last test, blood was collected into the EDTA tube. Thirty minutes before starting, each blood alcohol level was measured by a biochemical analyzer.

Plasma collected blood was left at room temperature for 30 minutes and centrifuged at 3000 xg for 15 minutes. The AST activity of plasma was measured using an AST kit (Boehringer Mannheim, Germany). Aspartic acid and α-ketoglutamic acid are converted to oxaloacetic acid and L-glutamic acid by the action of AST in plasma. The produced oxaloacetic acid was converted to malate by the action of MDH in the presence of coenzyme NADH, and the decrease in absorbance when NADH was oxidized to NAD + was measured by an automatic biochemical analyzer (Hitachi 747) at wavelength 340 nm.

Activity of ALT was measured by an automated biochemistry analyzer (Hitachi 747) using ALT (Boehringer Mannheim, Germany) kit. L-alanine and α-ketoglutamine acid are converted to pyruvic acid and L-glutamic acid by ALT action in plasma. The pyruvate produced was converted to lactate by the action of LDH in the presence of coenzyme NADH, and the absorbance decrease when NADH was oxidized to NAD + was measured by an automatic biochemical analyzer (Hitachi 747) at wavelength 340nm.

Triglyceride (TG) was measured by an autoanalyzer (Hitachi 747) using a TG kit (Boehringer Mannheim, Germany) by the glycerol non-erasing method, and cholesterol was measured using T. chol. Measurements were made with an automated analyzer (Hitachi 747) using a kit (Boehringer Mannheim, Germany).

MDA values were analyzed with a Spectrophotometer (HP8425A. Hewlette Packard.America) using an MDA kit (Bioxtech LPO-586 Assay, Oxis International, Inc. America). The active oxygen produced in the body combines with the fat in the body to produce lipid peroxide, and the lipid peroxide in the body acts as a causative agent of human aging in connection with various adult diseases. Was measured.

All experimental results were statistically analyzed using one-variable analysis method, and Student's T-test was used to verify the significance of each experimental group at p <0.5 level. All data are expressed as mean ± standard deviation.

Experimental Example 6: Anti-Fatigue Effect of Draft Macaric Acid Extract

Table 3 and Figure 3 shows the anti-fatigue effect of the raw malt extract extracted by the present invention. To evaluate the anti-fatigue effect by inducing the change in the level of lactic acid accumulated in the body by administering the liver function disorder substance, the treatment substance was fed to the high lactate level similar to the liver function obstacle feeding group. However, the lactic acid level of the animals fed the malt extract was suppressed to a nearly normal state, indicating that the anti-fatigue extract showed anti-fatigue activity.

Table 3. Anti-Fatigue Effects

Group Lactate Concentration (U / L) Mean ± S.D. Range (min-max) Sample 1 feed 1,059.75 ± 409.67 432-1,562 Sample 2 Feed + liver disorders 1,348.20 ± 465.55 845-1,959 Sample 3 Feed + liver function disorder + liver function therapeutic substance (hang hanger) 1,364.33 ± 176.93 1,098-1,497 Sample 4 Feed + Liver Function Disorder + Raw Wheat Extract 1,194.00 ± 251.33 847-1,496

(Each value represents the mean ± S.D. of 7rats)

Experimental Example 7: Alcohol Degradation Effect of Draft Macaric Acid Extract

Table 4 and Figure 4 shows the alcohol degradation effect of the raw machsan extract extracted by the present invention. 1 hour and 30 minutes after alcohol administration, Sample 3 showed the lowest value, but after 3 hours, blood alcohol concentration was significantly lower than that of other groups. By appearing, it can be seen that the alcohol-degrading action of the raw malt extract can prevent hangover and prevent fatty liver.

Table 4. Alcohol Degradation Effects

Group Alcohol concentration (%) 1 hour 30 minutes 3 hours Feed 0 0 Hazardous Substance Feeding 0.17 0.16 Hangover Eating 0.15 0.14 Feeding draft malic acid extract 0.17 0.11

(Each value represents the mean ± S.D. of 7rats)

Experimental Example 8: Plasma Triglyceride Content

Table 5 and Figure 5 shows the plasma triglyceride content. In the long-term negative alcohol control group, 73.71 ± 61.20 mg / dL showed a significant increase in plasma triglyceride content compared to 39.57 ± 8.62 mg / dL of the normal control group, indicating that hyperlipidemia was induced by long-term alcohol administration. It has been reported that long-term alcohol intake significantly increases triglyceride content, leading to fatty liver, and in particular chronic alcohol intake can lead to fatty liver, leading to malnutrition [Rahimtoola SH. (1985) Cholesterol and coronary heart disease. Am. Med. Assoc. 253: 2094-2095 and Castelli WP, Wilson PW, Levy D, Anderson K. (1990) Serum Lipids and risk of coronary artery disease. Athe roscl. Rev. 21: 7-10.] This leads to a decrease in food intake, especially the deficiency of anti-fatty factors such as protein, methionine, choline, vitamin E and Se [Cutta, S.K., P.A. Miller, L.B. Greenberg and O.A. Levander (1983) Celenium and acute alcoholism. Am. J. Clin. Nutr. 38: 713-718].

In the present invention, the T1 group showed a plasma triglyceride content of 30.20 ± 4.71 mg / dL, the T2 group 26.57 ± 1.68 mg / dL, and the T3 group 27.29 ± 6.96 mg / dL, compared with the negative control group T2 and T3 group These studies have shown significant (p <0.05, p <0.01) studies that significantly improve hyperlipidemia, which can be used to treat or improve hyperlipidemia very effectively with either raw malic acid extract (Mangmun-dong, Schizandra chinensis, Phosphoric acid, Astragalus, or Pumpkin) or fresh maize tofu. The results indicate that there is.

Experimental Example 9: Total Cholesterol Content in Plasma

Table 5 and Figure 6 shows the cholesterol content in the plasma. The plasma cholesterol content of the negative control group was increased to 113.80 ± 38.19 mg / dL compared to 95.71 ± 6.86 mg / dL of the normal group, indicating that hyperlipidemia was induced by long-term alcohol administration as in the case of the plasma triglyceride content. The T1 group had 86.17 ± 5.58 mg / dL, the T2 group had 85.00 ± 5.76 mg / dL, and the T3 group had 84.00 ± 9.20 mg / dL, significantly reducing cholesterol levels in all experimental groups (p <0.05). ), The results showed the possibility to expect excellent hyperlipidemia improvement and treatment effect.

High cholesterol levels in blood have been shown to be a major factor in the induction of cardiovascular diseases such as hypertension and atherosclerosis. Rahimtoola SH. (1985) Cholesterol and coronary heart disease. Am. Med. Assoc. 253: 2094-2095; Castelli WP, Wilson PW, Levy D, Anderson K. (1990) Serum Lipids and risk of coronary artery disease. Atheroscl. Rev. 21: 7-10; And Miettinen TA. (1987) Dietary fiber and lipids. Am. J. Clin. Nutr. 45: 1237 ~ 1242], many studies and efforts are being conducted worldwide to develop drugs and functional substances that lower the plasma cholesterol level of each individual. Especially, the above medicines or functional substances have been developed from natural products which have little side effects on the human body. The value added is expected to be enormous. In this regard, the results of this study offer potential as a novel natural hyperlipidemia treatment.

Table 5. Concentration of plasmid lipid in the rat.

Group TG (mg / dL) Mean ± S.D. CHOL (mg / dL) Mean ± S.D. No.1 (None-alcohol) 39.57 ± 8.62 ^** 92.80 ± 6.01 ^* No.2 (Alcohol) 73.71 ± 61.20 113.80 ± 38.19 No.3 (alcohol + hangover remover) 30.14 ± 6.73 91.57 ± 6.30 No.4 (T1) 30.20 ± 4.71 86.17 ± 5.58 ^* No.5 (T2) 26.57 ± 1.68 ^**** 85.00 ± 5.76 ^** No.6 (T3) 27.29 ± 6.96 ^*** 84.00 ± 9.20 ^**

(Each value represents the mean ± S.D. Of 7 rats)

Means with different superscript asterisks within a column and significantly different from each other at P <0.5 (*) and P <0.1 (**) and P <0.05 (***) and P <0.01 (****) as determined by Student's T-test.

Experimental Example 10: Changes in Plasma AST and ALT Enzyme Activities

Tables 6, 7 and 8 show plasma AST and ALT after administration of the sample for 4 weeks to examine the hepatic functional activity of the general tofu administration group (T1), the draft tofu administration group (T2), and the draft corn extract extract group (T3). AST is an enzyme involved in the process of producing oxaloacetate + glutamate in response to L-Aspartate + α-ketoglutarate and converting oxaloacetate to pyruvate, and ALT in response to DL-Alanine + α-krtoglutarate It is a transfer enzyme that produces glutamate. These enzymes are known as liver disease determinants because they indicate that liver function is decreased when their activity is increased.

As shown in Table 6 and FIG. 7, the AST level in the plasma of the normal control was 84.43 ± 47.88 U / L, but the AST level of the negative control group administered with alcohol for an extended period of time was 254.57 ± 463.20 U / L. Hepatic damage was induced by alcohol administration. In contrast, the T2 and T3 groups exhibited AST activities of 61.00 ± 3.78 U / L and 64.80 ± 6.24 U / L, which is very good when compared to the negative control and 70.29 ± 12.60 U / L of the positive control. Synergistic inhibitory effect was found. T1 group who received regular tofu with alcohol showed AST synergistic inhibitory effect with 78.40 ± 36.77 U / L, but did not show any significant (p <0.5).

Table 6 and Figure 8 shows the ALT enzyme activity. The ALT level of the normal group was 44.00 ± 9.04 U / L, whereas the negative control group was 215.43 ± 428.93 U / L, which was very high compared to the normal group, indicating that hepatic damage due to prolonged alcohol administration was confirmed. T2 and T3 groups were 34.14 ± 5.49 U / L and 36.40 ± 8.50 U / L, respectively (p <0.5), which were significantly lower than those of normal group. It did not reach the T3 group, but showed low activity with 37.83 ± 6.57 U / L.

In general, long-term intake of alcohol tends to increase the activity of AST and ALT, suggesting decreased activity of liver function. One of the first clinical manifestations in the liver when hepatic function is impaired is an increase in AST and ALT activity [Kien, C.L. and H.E. Ganther (1983) Manifestations of chronic selenium deficiency in a child receiving total parenteral nutrition. Am. J. Clin. Nutr. 37: 319-328. And Thompson, J.N. and M.L Scott (1970) Impaired lipid and Vitamin E absorption related to atrophy of the pancreas in selenium-deficient chicks. J. Nutr. 100: 797 to 809]. In particular, plasma ALT is widely used as a potent indicator of hepatobiliary tract disease because it is sensitive to hepatocellular degeneration and necrosis [Soon-Ho Kim, Han-Cheol Son, Eun-Yeop Lee, and Chul-Hoon Jang (1999). . 50 to 52].

Looking at the results shown in the present invention it can be seen that not only the Sangmasan extract, which is a combination of herbal medicine such as McMun-dong, Schisandra chinensis, Ginseng, Astragalus, pumpkin, etc. in the liver dysfunction caused by long-term alcohol intake, but also the saengmaek tofu has a significant recovery effect In addition, it was found that the long term administration significantly improved the recovery function of liver dysfunction than when the raw malic acid extract was administered alone.

Table 6. Enzyme activity of AST and ALT in plasma

Group AST (U / L) Mean ± S.D. ALT (U / L) Mean ± S.D. No.1 (None-alcohol) 84.43 ± 47.88 ^* 44.00 ± 9.04 ^* No.2 (Alcohol) 254.57 ± 463.20 215.43 ± 428.93 No.3 (alcohol + hangover remover) 70.29 ± 12.60 ^* 37.29 ± 9.30 ^* No.4 (T1) 78.40 ± 36.77 39.20 ± 9.22 ^* No.5 (T2) 61.00 ± 3.78 ^* 34.14 ± 5.49 ^** No.6 (T3) 64.80 ± 6.24 ^* 36.40 ± 8.50 ^*

(Each value represents the mean ± S.D. Of 7 rats)

Means with different superscript asterisks within a column and significantly different from each other at P <0.5 (*) and P <0.05 (**) as determined by Student's T-test.

Experimental Example 11: MDA content of plasma

Table 7 and Figure 9 shows the malone dialdehyde (MDA) content. The alcohol-only negative control group was 5.7 ± 3.24 microμmol, which significantly increased plasma MDA content compared to the normal control group's 4.40 ± 0.13, indicating that lipid peroxide production was induced by long-term alcohol administration. Reportedly, active oxygen in the body binds with body fat to produce lipid peroxide, and the lipid peroxide formed in the body is known as a cause of aging that promotes aging associated with various adult diseases.

The T1 group who received normal tofu showed a high value similar to the negative control group with 5.97 ± 1.91μmol, whereas the T2 group who received the DST was 4.82 ± 0.45μmol (P <0.1). Drafted tofu was shown to have antioxidant and anti-aging effects.

Table 7. Concentration of malondialdehyde in rat.

Group MDA (umol) Mean ± S.D. No.1 (None-alcohol) 4.40 ± 0.13 No.2 (Alcohol) 5.70 ± 3.24 ^* No.3 (alcohol + hangover remover) 4.56 ± 60.15 ^** No.4 (T1: Alcohol + regular tofu) 5.97 ± 1.91 ^** No.5 (T2: Alcohol + draft tofu) 4.82 ± 0.45 ^**

(Each value represents the mean ± S.D. Of 7 rats)

Means with different superscript asterisks within a column and significantly different from each other at P <0.5 (*) and P <0.1 (**) as determined by Student's T-test.

Experimental Example 12: Subacute Toxicity Test of Raw Macaric Acid Extracts

In order to test the subacute toxicity of bio-macroic acid extracts in vivo, experimental animals purchased Sprague-Dawley (SD) male 7-week-old male rats weighing 250 ± 10 g from the experimental animal center for 10 days in the animal nursery of Konkuk University. Adapted. During adaptation, the temperature and humidity of the feeding room were 22 ± 2 ℃ and 55 ± 5%, respectively, maintaining constant temperature and humidity. The diet was allowed to consume solid feed (Samyangsa) and water freely.

Six rats of about 300 g body weight adjusted to the rearing room for 10 days were divided into a normal control group and a test group. The normal control group was allowed to freely feed and water and orally administered 2ml of distilled water at 11:30 am daily. Experimental group 1 (hereinafter referred to as T1) allowed free feeding of food and water, and orally administered 2 ml of raw malic acid extract at 11:30 am daily. At this time, the diet freely supplied Samyang's solid feed, and the treatment of each group is shown in Table 8.

Table 8. Composition of Groups

Group No. of exam Treatment No.1 (Normal Control) 6 Feed + drinking water + distilled water No.2 (Test group1-T1) 6 Feed + drinking water + raw extract ^{1 )}

* Make Extracts ^{1 )} : Limoope platyphylla + Schizandra chinensis + Ginseng (Panax ginseng CA Meyer) + Astragalus membranaceus BUNGE + Pumpkin (Cucurbita spp.) Extract

Herbal medicine samples from Pandong ginseng CA Meyer (Gumsan, Chungcheongnam-do), Astragalus membranaceus BUNGE (Yangyang, Gangwon-do), Schizandra chinensis, Liriope platyphylla and Cucurbita spp. Mixed with 100 g of herbal medicine, add 1,000 ml of tertiary distilled water, boil for 2 hours and 30 minutes (Daewoong tangtang machine, DWP-2000M), filter it first with gauze, filter it with 3MM paper and filter the final volume 400 The solution was used while storing it in a 4 ° C. refrigerator. 400 ml of raw malic acid extract was calculated as the amount administered daily to humans, and 300 g rats were orally administered to 2 ml daily for 28 days.

In order to observe the general condition abnormality when administering the sample, the general condition of the animal was closely observed at least two times daily. As a general condition, the expression time, degree, and duration of abnormal condition were observed for skin, hair, eye, mucous membrane, bowel movement, movement, and pattern, and closely examined whether there is an increase in aggression against each other, self-harm, and tissue fusion. Observation checked. And the number of animals in each group was always checked to check the disappearance of test animals.

In order to observe the abnormality of each organ after necropsy, the color, size, and appearance of each organ in abdominal cavity were visually observed to check for abnormalities, and the liver and kidneys were preserved in the preservative solution.

To determine mortality, rat mortality was measured in each group for 28 days. In addition, for weight measurement (once a week) and organ weight measurement (after necropsy), the body weight was measured at the same time once a week for 4 weeks until the day before necropsy after administration of the sample. After finding and sampling the vena cava, the liver and kidney of each group of animals were accurately extracted and the weight of each group was measured to calculate the long-term rate of replacement. In order to measure the amount of feeding and the amount of feeding, the presence of abnormality was observed by measuring the feeding amount and the feeding amount of each group at the same time every day for 28 days once the sample administration.

Blood filled in the EDTA tube for hematological values (measurement of Hematological values and CBC differentiation) is well shaken and homogenized, and Coulter JT (coulter electronics Inc, USA, PN 4235846B) uses WBC (White Blood Cell), RBC (Red Blood Cell, Red Blood Cell), HGB (Hemoglobin, Hemoglobin), HCT (Hematocrit, Blood Volume Percentage), Platelets (Platelet) were measured. After soaking in ethanol for 12 hours, smear blood with a cotton swab on clean slide glass and dry it in air.10 drops of Wright stain (Yong Dong pharm, Co. Kyungki, Korea) solution is fixed and dyed for 3 minutes. (Yong Dong pharm, Co. Kyungki, Korea) 10 drops were mixed sufficiently and stained for 5-6 minutes. At this time, the discomfort in the speculum was minimized by careful not to settle the acupuncture. The slide glass was carefully washed with tertiary distilled water using a spray and dried at room temperature. After drying sufficiently, the specimen was examined without using a cover slide under oil immersion. Using the manual WBC differential counter, white blood cells were counted by type, counting the total number of white blood cells and counting the distribution as a percentage. The granularcytes were double checked with the coulter results. Reticulocyte frequency was dissolved in tertiary distilled water using capillary, filtered, and then mixed with 1% new methylene blue (sigma) stored at room temperature in the same amount with blood for 3 to 5 minutes and smeared on clean slide glass. It was. Reticuolcyte count represents the percentage of reticulocytes that appeared during counting 1000 red blood cells.

For blood biochemical tests (total protein, total cholesterol, albumin, AST, ALT, alkaline phosphatase, total bilirubin), the blood filled in the EDTA freed tube was left at room temperature for 30 minutes and then centrifuged at 3000 × g for 15 minutes. Aspartate aminotransferase (AST) activity of the obtained serum was measured by an automatic biochemical analyzer (Hitachi 747) using the AST kit (Boehringer Mannheim, Germany). The activity of Alanine aminotransferase (ALT) was measured by an automated biochemistry analyzer (Hitachi 747) using ALT (Boehringer Mannheim, Germany) kit. Total cholesterol (T-Chol.) Was measured by T. chol. Measurements were made with an automated biochemistry analyzer (Hitachi 747) using a kit (Boehringer Mannheim, Germany). Albumin combines with BCG (Brom Cresol-Green) to form a compound at pH 4.2. The absorbance was measured by an automatic biochemistry analyzer (Hitachi 747) using ALB (Boehringer Mannheim, Germany) kit.

Alkaline phosphatase (ALP) was measured by an automated biochemistry analyzer (Hitachi 747) using the ALP kit (Boehringer Mannheim, Germany) under the IFCC test principle. Total bilirubin (T-Bil.) Was measured by an automated biochemistry analyzer (Hitachi 747) using Bil-T (Boehringer Mannheim, Germany) Kit under the DPD method. Total protein (TP) was measured using an automated biochemistry analyzer (Hitachi 747) using the TP kit (Boehringer Mannheim, Germany) under the Biuret method.

Before each autopsy for urine tests (measured pH, urine occult blood, urine protein, urine glucose, and urine ketone), one animal from each group was placed in an ethanol sterilized cage and waited for urine to be discharged. Then, the discharged urine was put in a sterile cap tube, and then the degree of urine pH, urine protein, urine glucose, urine ketone and urinary occult blood of each experimental animal was measured by using a test paper (Serotech Korea Co. Ltd., Seoul). Inspected.

Animals were fasted for 16 hours overnight and weighed on the last day of breeding. SD rats weighed after weighing were placed in a 25 cm diameter desiccator, anesthetized with distilled anhydrous ether (TJ Baker Analized ACS reagent, USA) for 3 to 4 minutes, and ventral was visible from the dissection. Place and secure the four legs with thumb tags. After disinfecting the entire abdomen with alcohol, the abdominal skin was picked up with forcep, dissected along the median line with dissection scissors, and the incision was visible with an anatomical knife. Wash with 0.85% saline (Shinyo pure chemicals Co. LTD, Japan), and collect 5 ~ 6ml of blood from posterior vena cava by using syringe and test tube without EDTA while spraying saline sufficiently to prevent organs from drying out. After 30 minutes at, low-temperature (4 ℃) centrifuged for 15 minutes at 3000 rpm to obtain a serum. Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), alkaline phosphatase (ALP), total cholesterol, total protein, albumin and total bilirubin were used. EDTA blood obtained from blood collection in the EDTA tube was used to measure hematological values and CBC differentiation. Liver and kidneys were excised and weighed and fixed in buffered formalin solution (10 ml of 40% formaldehyde, 0.65 g sodium phosphate monobasic, sigma, 0.4 g sodium phosphate dibasic, sigma / 100 ml T.D.W) and stored at room temperature.

All experimental results were statistically analyzed using one-variable analysis method, and Student's T-test was used to verify the significance between each experimental group at p <0.05 level. All data are expressed as mean ± standard deviation.

The general condition of the test animals was closely observed at least twice daily after sample administration. No abnormal clinical symptoms attributable to the test substance during the sample administration were observed. In other words, no abnormalities were observed in skin, hair, eye, mucosa, bowel movement, movement and pattern, and no abnormal signs such as aggression to other animals, self-injury to oneself, and tissue fusion were found.

On the last day of breeding, the animals were fasted overnight for 16 hours and anesthetized with ether for 3 to 4 minutes. After autopsy, the normal control group and each experimental group had heart, lung, liver, kidney, spleen, adrenal, stomach and testis abnormalities. Was observed. The organs of each experimental group showed no apparent abnormal change in shape, shape, size, color, etc. compared to the organs of the normal control group, and the color and shape of each organ were observed to be very clear. No ideal specific symptoms were considered. During the 4 weeks after the administration of the sample, the amount of intake and intake of the T1 group to which the normal control group and the raw machlic acid extract were administered were constant and showed no ups and downs, and there were no experimental animals that died from the normal control group and the experimental group.

The weekly weight change for 4 weeks after the administration of Sprague-Dawley rats (males) was not significantly different from that of the normal control group. The total weight gain for 4 weeks after the sample administration also showed no significant difference (p <0.05) compared to the normal control group as shown in Table 9. That is, the weekly weight change and total weight gain of the experimental animals during the four weeks of the administration of the sample showed that the T1 showed similar values to the normal group, indicating that the administration sample did not have a significant effect on the increase or decrease of the body weight. The long-term weight change of the experimental animals is shown in Table 9. The percent liver weight per body weight of the experimental animals showed a value of 2.94 ± 0.077 in the normal control group, but there was no significant difference (p <0.05) in all the experimental groups. Showed. The percent kidney weight per body weight of the experimental animals also did not show a significant difference (p <0.05) in all the experimental groups compared to the normal control group with a value of 0.711 ± 0.043.

Table 9. Total body weight gains and the weight ratio of liver and kidney

 Group Total body weight gains (g) Mean ± S.D. Liver (% of body weight) Mean ± S.D. Kidney (% of body weight) Mean ± S.D. No.1 (Normal) 120.17 ± 7.84 2.94 ± 0.077 0.711 ± 0.043 No.2 (T1) 120.20 ± 12.86 3.09 ± 0.279 0.717 ± 0.039

(Each value represents the mean ± S.D. Of 6 rats)

Significant differencesas compared with control: P <0.05

Table 10 shows the routine urinalysis results for urine collected from experimental animals of each group (n = 6). In general, the change of urine in the sample reflects the condition of the kidney and urinary system well, so it is useful for the diagnosis of prognosis, and since it reflects the change of body fluid, it is an indicator of metabolic disorder, diabetes, liver disease, unbalance of electrolyte.

The urine pH of the normal control group and the experimental group shown in Table 10 ranged from 7.5 to 8.5, indicating no difference between the normal group and the experimental group. The specificity of the urine pH due to the administration sample was also observed in accordance with the criteria of urinalysis. Did.

The urinary protein of each animal was normal from negative (-) to trace (±) on the basis of urinalysis. However, in this experiment, one out of six T1 group showed traces in 1 and 4 in 1 positive and 2 in 1 positive. In the normal control group, urine protein was detected in almost all experimental animals such as traces in 1 body, 1 positive in 3, and 2 positive in 1. The positive results in both the normal control group and the experimental group could not be judged that the administration sample caused urine protein abnormality. In general, if the urine protein of the normal control group is negative while the urine protein of the test group is positive, pathological proteinuria such as glomerular proteinuria, tubuloproteinuria, kidney and renal proteinuria due to the administration drug may be suspected. When proteinuria is detected in all sample-administered groups, it can be determined as physiological proteinuria (non-pathological proteinuria) such as motor proteinuria and functional proteinuria. In this study, proteinuria was detected in both the normal control group and the experimental group. It was judged that active physiological proteinuria was detected by collecting urine at night in the nocturnal rats. Supports the determination that exercise caused physiological proteinuria.

The detection of glucose in the urine of the sample reflects signs of diabetes mellitus, nephrotic diabetes, endocrine disease, pancreatic disease, cirrhosis and brain tumor. As shown in Table 10, no sugar was detected in both the normal control group and the T1 group to which the raw malic acid extract was administered, and the results of the study showed no abnormal state of urine glucose due to the administration sample.

Table 10 shows the results of urine ketone measurement in the normal control and experimental groups. Urine ketones are a key indicator for diagnosing fatty acid hyperactivity. Ketones increase in dependence on fatty acids rather than sugars and their increase reflects insulin deficiency. Thus, the detection of ketones in the urine of a sample indicates that the sample is starved in severe diabetes or severely inadequate glucose intake. The results of this study on urine ketone showed 1 positive in 2 of 6 normal control rats and 1 positive in T1 group. As described above, the positive result in both the normal control group and the experimental group could not be judged that the administration sample caused the urine ketone body abnormality. Rather, based on the fact that the experimental animals ingested sufficient diets and no urine glucose was detected in all experimental groups, the composition of the Samyangsa diets consumed by the animals (more than 22.1% of crude protein, more than 3.5% of crude fat, less than 5.0% of crude fiber, It was judged that the inquiry content was 8.0% or less…). In other words, the ratio of crude fiber of carbohydrates in the rats of the normal control group and the experimental group was extremely low, and the ratio of crude fat and crude protein was 5, 6 times higher than that of the crude fiber. To obtain more accurate results, it is necessary to feed after changing feeds and retest. Urinary occult blood is an indicator for diagnosing urinary tract abnormalities. Table 10 shows the results of urinary occult blood in the normal control and experimental groups. Urinary occult blood was not detected in each group. Therefore, it was judged that abnormal state of urinary occult blood due to the administration sample did not appear.

Table 10. Urinary analysis

pH Protein (U) Glucose (U) Ketone (U) Blood (U)   No.1 (Normal) 7.5 1Positive Negative Negative Trace (±) 8.5 Trace (±) Negative 1Positive Negative 8.5 1Positive Negative Negative Negative 8.5 2Positive Negative 1Positive Negative 8.5 Negative Negative Negative 8.5 1Positive Negative Negative Negative   No.2 (T1) 8.0 2Positive Negative Negative Negative 8.5 1Positive Negative 1Positive Negative 8.5 1Positive Negative Negative Negative 8.5 Trace (±) Negative Negative Negative 8.5 1Positive Negative Negative Negative 8.5 1Positive Negative Negative Negative

Table 11 shows the results of hematological values and CBC differentiation measurements after ± 4 weeks of administration of the test substance. RBC, WBC, HCT, HGB, PLT, Reticulocyte, WBC diff. As a result of counting statistically by Student's T-test, there was a slight difference in each experimental group, but no group showed a significant difference (p <0.05) compared to that of the normal group. Therefore, the study showed no hematologic toxicity due to the administration sample.

Table 11.Hematological results

No.1 (Normal) No.2 (T1) RBC 7.86 ± 0.13 7.78 ± 0.66 WBC 9.97 ± 1.57 9.42 ± 2.79 BCT 47.80 ± 3.19 46.20 ± 4.40 HGB 15.28 ± 0.17 14.80 ± 1.27 PLT 1072 ± 188.71 1033.40 ± 75.67 Retieylocyte 3.26 ± 0.25 3.23 ± 0.29 Neutrophil Stab. - - Neutrophil Seq. 8.60 ± 3.38 8.00 ± 0.71 Lymphocyte 88.20 ± 5.56 88.20 ± 2.32 Monocyte 3.20 ± 2.18 3.60 ± 1.50 Eosinophil - - Basophil - - Normoblast - - Blast - -

(Each value represents the mean ± S.D. Of 6 rats)

Significant differencesas compared with control: P <0.05

RBC, red blood cell count (10 ⁴ / mm ³ ); WBC, white blood cell count (10 ³ / mm ³ ); HCT, hematocrit (%); HGB, hemoglobin (g / dL); PLT, platelet (10 ³ / mm ³ )

Table 12 shows the results of blood biochemical test after administration of test substance for 4 weeks. TP, albumin, T-Bil, ALP, AST, ALT, T-Chol. As a result of statistical analysis, there was a slight difference in each experimental group, but no group showed a significant difference (p <0.05) compared to that of the normal group. Therefore, it was judged that there was no blood biochemical toxicity due to the administration sample.

Table 12. Biochemical values

No.1 (Normal) No.2 (T1) TP 6.27 ± 0.23 6.35 ± 0.13 Albumin 3.75 ± 0.14 3.82 ± 0.09 T-Bil. 0.1 ± 0.00 0.1 ± 0.00 ALP 95.00 ± 5.77 100.80 ± 4.79 AST 111.33 ± 35.42 104.17 ± 24.45 ALT 40.33 ± 6.07 39.83 ± 3.48 T-Chol 75.67 ± 4.82 75.83 ± 13.58

(Each value represents the mean ± S.D. Of 6 rats)

Significant differencesas compared with control: P <0.05

TP, total protein (mg / dL); ALP, alkaline phosphotase (U / L);

ASP, aspatate aminotransferase (U / L); ALT, alanine aminotransferase (U / L); T-Chol, tota cholesterol (U / L); T-Bil, total bilirubin (mg / dL)

As described above, in the present invention, the raw malt extract, which is obtained by combining herbal medicines such as Macmundong, Schisandra chinensis, Ginseng, Astragalus, and pumpkin, may exhibit anti-fatigue effect by hydrolysis of lactic acid, and alcohol degrading effect, which significantly reduces blood alcohol concentration. In addition, the tofu coagulated with this vegetative acid extract lowers the concentrations of blood cholesterol and triglycerides, which are the main factors for cardiovascular diseases such as hypertension and arteriosclerosis. It can be seen that the function and antioxidant activity to prevent various adult diseases and aging is significantly increased than when the raw malic acid extract alone was administered, and much research and efforts around the world have been made to develop drugs and functional substances that can exhibit such effects. It is progressing, especially from natural substances with few side effects In the development of medicines, or the functional material, and is a very useful invention that can be developed as an anti-fatigue effect of the new and alcoholysis effect and anti-hyperlipemia effect and increase liver function activity, an anti-oxidant.

Claims (2)

Each weight ratio is 2.6 to 5.0%, Mammundong 2.6 to 5.0%, Astragalus 2.6 to 5.0%, Ginseng 1.0 to 5.0%, Pumpkin 2.6 to 5.0% and distilled water 75 to 87%, 95 to 95 hours for 2 to 5 hours Raw machinate extract, characterized in that the extract is concentrated by heating in a bath at a temperature of 130 ℃, filtered with gauze, centrifuged for 10 to 30 minutes at a speed of 5000 ~ 8000 × g in a centrifuge. Each weight ratio is 2.6 to 5.0%, Mammundong 2.6 to 5.0%, Astragalus 2.6 to 5.0%, Ginseng 1.0 to 5.0%, Pumpkin 2.6 to 5.0% and distilled water 75 to 87%, 95 to 95 hours for 2 to 5 hours Extracted the concentrated liquid by heating in a shaker to a temperature of 130 ℃, filtered with gauze, centrifuged in a centrifuge for 10 to 30 minutes at a speed of 5000 ~ 8000 × g, and extracted with the extract of draft malt, soybean 10% (v / v) Tofu maize tofu using the saengmaek extract, characterized in that the 95% to 97% (v / v) soy milk solution is prepared by mixing the 3 to 5% (v / v).

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