KR20050050869A - A feed additive for a livestock - Google Patents

A feed additive for a livestock Download PDF

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KR20050050869A
KR20050050869A KR1020030084518A KR20030084518A KR20050050869A KR 20050050869 A KR20050050869 A KR 20050050869A KR 1020030084518 A KR1020030084518 A KR 1020030084518A KR 20030084518 A KR20030084518 A KR 20030084518A KR 20050050869 A KR20050050869 A KR 20050050869A
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weight
parts
additive
livestock
livestock feed
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계기수
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(주)삼조생명과학
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/845—Rhizopus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Fodder In General (AREA)

Abstract

본 발명은 가축 사료용 첨가제에 관한 것으로, 상기 첨가제는 리조푸스 0.05 내지 5.0 중량부, 비오틴 0.01 내지 1.0 중량부, 아연 0.2 내지 20 중량부, 크롬 0.006 내지 0.60 중량부, 구리 0.021 내지 2.1 중량부 및 코발트 0.001 내지 0.05 중량부를 혼합하여 제조되며, 표피세포를 재생시키고 가축발굽을 건강하게 하며 가축 사료의 효율을 증가시키므로, 가축의 피부질환 또는 발굽질환의 예방이나 치료에 유용하게 사용될 수 있다. The present invention relates to an additive for livestock feed, wherein the additive is 0.05 to 5.0 parts by weight of lyfuose, 0.01 to 1.0 parts by weight of biotin, 0.2 to 20 parts by weight of zinc, 0.006 to 0.60 parts by weight of chromium, 0.021 to 2.1 parts by weight of copper and cobalt. It is prepared by mixing 0.001 to 0.05 parts by weight, and regenerates the epidermal cells, makes the animal's hooves healthy, and increases the efficiency of the animal feed, and thus can be usefully used for the prevention or treatment of skin diseases or hoof diseases of the animals.

Description

가축 사료용 첨가제{A feed additive for a livestock}A feed additive for a livestock}

본 발명은 가축 사료용 첨가제에 관한 것으로, 상기 첨가제는 리조푸스 0.05 내지 5.0 중량부, 비오틴 0.01 내지 1.0 중량부, 아연 0.2 내지 20 중량부, 크롬 0.006 내지 0.60 중량부, 구리 0.021 내지 2.1 중량부 및 코발트 0.001 내지 0.05 중량부로 구성되는 가축 사료용 첨가제에 관한 것이다.The present invention relates to an additive for livestock feed, wherein the additive is 0.05 to 5.0 parts by weight of lyfuose, 0.01 to 1.0 parts by weight of biotin, 0.2 to 20 parts by weight of zinc, 0.006 to 0.60 parts by weight of chromium, 0.021 to 2.1 parts by weight of copper and cobalt. It relates to an additive for livestock feed composed of 0.001 to 0.05 parts by weight.

일반적으로, 동물은 몸에 상처가 생길 경우, 그 상처 부위를 어느 정도 스스로 방어하고 자가치유를 하려고 하는 성질을 가지고 있다. 특히, 창상과 같은 외상에 의하여 피부가 손상될 경우, 일정 수준의 이하의 피부손상은 자가치유 능력으로 회복될 수 있으나, 일정 수준 이상의 피부손상은 세균의 감염 등과 같은 기타 요인으로 인하여, 다른 질병으로 확산될 수도 있다. 이를 방지하기 위하여, 생체 친화성이 우수하여 상처 부위에 있어서의 거부반응이 적고, 상처 부위와 밀착할 수 있어야 하며, 상처 부위의 세균 감염을 방지할 수 있어야 하고, 다른 물질과 혼합하여 사용하더라도 반응성이 없는 첨가제의 개발이 요구된다. In general, when an animal has a wound, it has a property of defending itself to some extent and trying to heal itself. In particular, when the skin is damaged by a trauma such as a wound, the skin damage below a certain level can be recovered by the self-healing ability, but the skin damage above a certain level may be caused by other diseases due to other factors such as bacterial infection. It may also spread. In order to prevent this, it has excellent biocompatibility, so there is little rejection reaction at the wound site, it should be able to adhere closely to the wound site, it should be able to prevent bacterial infection at the wound site, and reactive even if mixed with other substances Development of a free additive is required.

한편, 발굽 질환으로는 양, 염소, 말, 젓소 및 육우 등과 같은 가축에 유모상(hairy) 발굽 종기(유두종성 발가락 피부염), 발굽 부패증(지간 결합조직염) 또는 만성 발굽 부패증(지간 피부염) 등이 알려져 있다. On the other hand, hoof diseases include hairy hoof boils (papillary toe dermatitis), hoof rot (interhepatic connective tissue) or chronic hoof rot (cludal dermatitis) in livestock such as sheep, goats, horses, cows and beef cattle. Etc. are known.

발굽 종기는 1974년에 이탈리아에서 처음 보고되었고, 이후 전세계에 만연되었는데, 1980년대 후반 이래 발굽 종기는 소의 절름발이의 중요한 원인으로 알려졌고, 낙농 산업에 큰 경제적 타격을 주었다. 예를 들어, 미국 농무부의 최근 연구 결과에 의하면 미국에서의 모든 낙농 가축들의 47 ％가 발굽 종기에 의해 감염되며, 이들의 78 ％가 1993년 이후에 발생한 것으로 보고되었다(Digital Dermatitis on U.S. Dairy Operations, NAHMS Dairy Study, May, 1997 참조). 젖소의 경우, 발굽 종기는 또한 우유 생산, 재생율 및 체중에서의 손실과 관련된다. Hoof boils were first reported in Italy in 1974, and have since become widespread worldwide. Since the late 1980s, hoof boils have been known to be a major cause of lame cattle and have caused significant economic harm to the dairy industry. For example, a recent study by the US Department of Agriculture reported that 47% of all dairy cattle in the United States were infected with hoof boils, and 78% of them occurred after 1993 (Digital Dermatitis on US Dairy Operations, NAHMS Dairy Study, May, 1997). In the case of cows, hoof boils are also associated with milk production, regeneration and loss in weight.

또, 발굽 부패증은 발가락의 발톱 사이의 연조직의 감염으로, 혐기성 박테리아인 푸소박테륨 네크로포럼(Fusobacterium necrophorum)에 의해 발병되며, 치료되지 않고 방치될 경우에는 발가락의 관절, 뼈 또는 힘줄에 들어가 감염으로부터 회복되기가 어려워진다. 발굽 부패증이 있는 동물은 미열, 식욕 감퇴 및 부수적인 체중 감량이 생기고, 약간의 절름발이에서 심각한 절름발이로 진행된다. Hoof rot is also an infection of the soft tissues between the toenails of the toes, caused by the anaerobic bacterium Fusobacterium necrophorum, which, if left untreated, enters the joints, bones or tendons of the toes. It is difficult to recover from. Animals with hoof rot develop mild fever, loss of appetite and concomitant weight loss, and progress from slight lame to severe lame.

또, 만성 발굽 부패증은 일반적으로 발끝 사이의 부위 즉, 지간 갈라진 틈의 피부에 존재하는 만성적인 염증으로, 박테리아 디켈로박터 노도수스에 의해 발병되며, 지간 갈라진 틈 부위의 피부는 딱딱한 표면을 형성하는 건조 삼출이 있는 종창으로 나타난다. 만성 발굽 부패증은 종종 절름발이 또는 발꿈치 균열/뒷발굽 부식을 일으킬 수 있지만, 일반적으로 동물의 걸음걸이의 변형을 야기한다.In addition, chronic hoof rot is generally a chronic inflammation in the area between the toes, that is, the skin of the bony cleft, which is caused by the bacterium Decelobacter nordosus, and the skin of the bony crevice forms a hard surface. To a swollen swelling with dry exudation. Chronic hoof rot can often cause lame or heel cracking / hind hoof erosion, but generally causes deformation of the gait of the animal.

현재, 발굽 종기, 발굽 부패증 및 만성 발굽 부패증 등과 같은 발굽 질환은 항생제, 예를들어 테트라시클린, 린코마이신, 스펙티노마이신, 페니실린, 옥시테트라시클린 또는 암피실린 등이 족욕(footbath), 분무 또는 발싸개를 사용하여 손상된 부위에 국소적으로 적용된다. 하지만, 항생제는 비싸고, 특히 젖소와 관련하여 항생제의 사용은 동물 또는 우유에 항생제의 잔류물이 존재하게 할 수 있고, 광범위한 항생제의 사용은 항생제 내성이 있는 박테리아 균주의 발전을 일으킬 수 있으므로, 발굽 질환 치료를 위한 새로운 대안이 필요한 실정이다.Currently, hoof diseases, such as hoof boils, hoof rot and chronic hoof rot, are treated with antibiotics such as tetracycline, lincomycin, spectinomycin, penicillin, oxytetracycline or ampicillin, such as footbath, spraying. Or topically applied to the damaged area using a foot wrap. However, hoof disease is expensive because antibiotics are expensive and the use of antibiotics, especially in relation to cows, can result in the presence of antibiotic residues in animals or milk, and the use of a wide range of antibiotics can lead to the development of antibiotic resistant bacterial strains. There is a need for new alternatives for treatment.

이에, 본 발명자들은 표피세포를 재생시키고, 가축발굽을 건강하게 하며 운동능력을 향상시키는 가축 사료용 첨가제를 개발하던 과정에서, 리조푸스 0.05 내지 5.0 중량부, 비오틴 0.01 내지 1.0 중량부, 아연 0.2 내지 20 중량부, 크롬 0.006 내지 0.60 중량부, 구리 0.021 내지 2.1 중량부 및 코발트 0.001 내지 0.05 중량부로 구성되는 가축 사료용 첨가제를 개발하여 본 발명을 완성하였다.Thus, the present inventors in the process of developing the additives for livestock feed that regenerates the epidermal cells, makes the animal's hooves healthy and improves the athletic ability, 0.05 to 5.0 parts by weight of Rifupus, 0.01 to 1.0 parts by weight biotin, 0.2 to 20 zinc The present invention has been completed by developing an additive for livestock feed consisting of parts by weight, 0.006 to 0.60 parts by weight of chromium, 0.021 to 2.1 parts by weight of copper and 0.001 to 0.05 parts by weight of cobalt.

본 발명의 목적은 표피세포를 재생시키고 가축발굽을 건강하게 하며 가축 사료의 효율을 증가시킴으로써 피부질환 또는 발굽질환을 예방하거나 치료할 수 있는 가축 사료용 첨가제를 제공하는 것이다.It is an object of the present invention to provide an additive for livestock feed that can prevent or treat skin diseases or hoof diseases by regenerating epidermal cells, making healthy animal hooves and increasing the efficiency of animal feed.

상기 목적을 달성하기 위하여, 본 발명은 리조푸스 0.05 내지 5.0 중량부, 비오틴 0.01 내지 1.0 중량부, 아연 0.2 내지 20 중량부, 크롬 0.006 내지 0.6 중량부, 구리 0.021 내지 2.1 중량부, 코발트 0.001 내지 0.05 중량부로 구성되는 가축 사료용 첨가제를 제공한다.In order to achieve the above object, the present invention is 0.05 to 5.0 parts by weight of lyfupus, 0.01 to 1.0 parts by weight of biotin, 0.2 to 20 parts by weight of zinc, 0.006 to 0.6 parts by weight of chromium, 0.021 to 2.1 parts by weight of copper, 0.001 to 0.05 parts of cobalt It provides an additive for livestock feed consisting of parts by weight.

보다 바람직하게는, 리조푸스 0.08 내지 3.0 중량부, 비오틴 0.05 내지 0.5 중량부, 아연 0.5 내지 10 중량부, 크롬 0.01 내지 0.3 중량부, 구리 0.05 내지 1.5 중량부 및 코발트 0.001 내지 0.02 중량부로 구성되는 가축 사료용 첨가제를 제공한다.More preferably, livestock comprising 0.08 to 3.0 parts by weight of lyfupus, 0.05 to 0.5 parts by weight of biotin, 0.5 to 10 parts by weight of zinc, 0.01 to 0.3 parts by weight of chromium, 0.05 to 1.5 parts by weight of copper and 0.001 to 0.02 parts by weight of cobalt Provide feed additives.

가장 바람직하게는, 리조푸스 0.1 내지 1.0 중량부, 비오틴 0.05 내지 0.3 중량부, 아연 1 내지 10 중량부, 크롬 0.01 내지 0.1 중량부, 구리 0.05 내지 1.0 중량부 및 코발트 0.001 내지 0.01 중량부로 구성되는 가축 사료용 첨가제를 제공한다.Most preferably, livestock comprising 0.1 to 1.0 parts by weight of lyfupus, 0.05 to 0.3 parts by weight of biotin, 1 to 10 parts by weight of zinc, 0.01 to 0.1 parts by weight of chromium, 0.05 to 1.0 parts by weight of copper and 0.001 to 0.01 parts by weight of cobalt. Provide feed additives.

이하, 본 발명을 상세히 설명한다. Hereinafter, the present invention will be described in detail.

리조푸스(Rhizopus)는 뇌하수체 전엽호르몬의 분비를 촉진하여 난소와 자궁의 활력을 유도하여 번식기능을 증진시키고, 미량미네랄의 흡수를 증가시켜 에너지 대사의 준비율을 높여주며, 단백질 및 에너지 대사의 불균형을 향상시킨다. 시상하부-뇌하수체계(hypothalamo-hypophysial system)을 통하여 갑상선자극호르몬(thyreoid stimulating hormone)을 분비하여 갑상선에서 갑상선호르몬 예를들어, 티록신(thyroxin)과 트리이오도티로닌(triiodothyronine)의 분비를 촉진하여 에너지 대사의 균형을 유지하도록 한다.Rhizopus promotes the secretion of anterior lobe of pituitary gland, induces vitality of the ovary and uterus, improves reproductive function, increases absorption of trace minerals, increases the readiness of energy metabolism, and improves imbalance of protein and energy metabolism. Improve. Thyroid secretion is released through the hypothalamic-hypophysial system to stimulate the secretion of thyroid hormones, such as thyroxin and triiodothyronine, from the thyroid gland. Balance your metabolism.

비오틴은 비타민 H라 불리고도 하며, 혈당강하작용, 인슐린과 유사한 작용으로 혈중 포도당을 세포로 이동시켜주는 기능과 세포내에서 당이 분해되어 에너지를 공급할 수 있도록 준비해 주는 내당인자(glucose tolerance factor)의 기능을 나타낸다.Biotin, also called vitamin H, is a hypoglycemic, insulin-like action that transports glucose into the cell and acts as a glucose tolerance factor that prepares the cells to break down and provide energy. Indicates a function.

아연은 킬레이트미네랄로, 탁월한 혈당강하작용과 에너지대사의 필수단계에 작용하는 카르복실화(carboxylation)에 관여하는 효소인 카르복실레이제(carboxylase)의 조효소로서 작용하므로, 절대 결핍되어서는 안 되는 에너지대사의 중요한 인자이고, 크롬은 내당인자로서의 기능이 알려져 있고, 구리는 골격형성, 연골과 건 형성에 관여하고, 철분대사에 영향을 주어 헤모글로빈을 형성하는 과정에 조효소로 관여하며, 구리 부족시에는 철분이 충분히 공급되어도 빈혈을 발생시키며, 코발트는 비타민 B12의 구성요소이다.Zinc is a chelate mineral that acts as a coenzyme of carboxylase, an enzyme that participates in carboxylation, which plays an important role in blood sugar lowering and energy metabolism. It is an important factor of metabolism, and chromium is known as an endogenous sugar factor, copper is involved in skeletal formation, cartilage and tendon formation, affects iron metabolism as a coenzyme in the process of forming hemoglobin, and in the case of copper deficiency An adequate supply of iron causes anemia, and cobalt is a component of vitamin B12.

본 발명의 가축 사료용 첨가제는 표피세포를 재생시키는 효과가 탁월하여 각종 피부질환의 예방 또는 치료에 유용하게 사용될 수 있다.The additive for livestock feed of the present invention has an excellent effect of regenerating epidermal cells and can be usefully used for the prevention or treatment of various skin diseases.

또한, 본 발명의 가축 사료용 첨가제는 가축발굽을 건강하게 하는 효과가 탁월하여 각종 발굽질환의 예방 또는 치료에 유용하게 사용될 수 있다.In addition, the additives for livestock feed of the present invention is excellent in the effect of making the livestock hooves can be usefully used for the prevention or treatment of various hoof diseases.

또한, 본 발명의 가축 사료용 첨가제는 세포내 포도당 준비율의 상승과 에너지 대사의 개선으로 인해 가축 사료의 효율을 증가시킬 수 있다.In addition, the additive for livestock feed of the present invention can increase the efficiency of livestock feed due to the increase in the intracellular glucose preparation rate and the improvement of energy metabolism.

본 발명에 따른 가축 사료용 첨가제는 경구투여 또는 경피투여를 위해 고형 제제 또는 액체 제제로 사용하며, 가축 사료용 첨가제에 전분, 수크로스, 락토오스, 젤라틴 등과 같은 부형제나 스테아린산 마그네슘(Magnesium stearate), 탈크(talc) 등과 같은 활택제를 추가로 포함하여 사용할 수도 있다. 투여단위는 예를들어, 개별 투여량의 1, 2, 3 또는 4배를 함유하거나 또는 1/2, 1/3 또는 1/4배를 함유할 수 있고, 개별 투여량은 바람직하기로는 가축 사료용 첨가제가 1회에 투여되는 양을 함유하며, 이는 통상 1일 투여량의 전부, 1/2, 1/3 또는 1/4배에 해당한다. The additives for livestock feed according to the present invention are used as solid preparations or liquid preparations for oral or transdermal administration, and excipients such as starch, sucrose, lactose, gelatin, magnesium stearate, talc (talc), etc. It may also be used in addition to a lubricant such as). Dosage units may contain, for example, 1, 2, 3 or 4 times the individual dose or may contain 1/2, 1/3 or 1/4 times, and the individual dose is preferably for livestock feed. The additive contains an amount administered in one dose, which usually corresponds to all, 1/2, 1/3 or 1/4 times the daily dose.

표피세포를 재생시키고 가축발굽을 건강하게 하며 가축 사료의 효율을 증가시킴으로써 피부질환 또는 발굽질환을 예방하거나 치료할 수 있는 가축 사료용 첨가제의 유효용량은 3.5 내지 60 ㎎/㎏이고, 바람직하기로는 20 내지 50 mg/kg이며, 하루 1-6 회 투여될 수 있다. 단, 특정 가축에 대한 유효용량 수준은 가축의 체중, 연령, 성별, 건강상태, 식이, 급여시간, 급여방법, 배설율, 질환의 중증도 등에 따라 변화될 수 있다. The effective dose of the additive for livestock feed which can prevent or treat skin disease or hoof disease by regenerating epidermal cells, making the livestock hooves healthy and increasing the efficiency of the livestock feed is 3.5 to 60 mg / kg, preferably 20 to 50 mg / kg and may be administered 1-6 times a day. However, the effective dose level for a particular livestock may vary depending on the weight, age, sex, health status, diet, feeding time, feeding method, rate of excretion, and severity of the disease.

특히, 본 발명에 따른 가축 사료용 첨가제에 대한 독성시험 결과, 50％ 치사량(LD50)은 적어도 가축 사료용 첨가제 50 g/kg 이상인 것으로 확인되어 가축 사료용 첨가제는 안정한 물질로 판명되었다.In particular, as a result of the toxicity test for livestock feed additives according to the present invention, 50% lethal dose (LD 50 ) was found to be at least 50 g / kg additives for livestock feed, so the additives for livestock feed proved to be a stable substance.

이하, 본 발명을 실시예에 의해 상세히 설명한다. Hereinafter, the present invention will be described in detail by way of examples.

단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다. However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

<실시예 1> 가축 사료용 첨가제의 제조 Example 1 Preparation of Additives for Livestock Feed

리조푸스 단위 분말 0.625g, 비오틴 0.1125g, 아연 메티오네이트 2.3625g, 크롬 트리피콜리네이트 0.0703g, 구리 메티오네이트 0.2363g 및 코발트 메티오네이트 0.0047g를 혼합하여 본 발명의 가축 사료용 첨가제를 제조하였다. An additive for livestock feed of the present invention was prepared by mixing 0.625 g of lycopus unit powder, 0.1125 g of biotin, 2.3625 g of zinc methionate, 0.0703 g of chromium tripicolinate, 0.2363 g of copper methionate, and 0.0047 g of cobalt methionate. It was.

<실험예 1> 세포재생 효과 검토Experimental Example 1 Review of Cell Regeneration Effect

8주령(체중 2 Kg)의 건강한 웅성 가토(New Zealand White Rabbit, 대한 동물 사육 센터)를 사육실 조건에서 사료(제일유지 사료)와 물(drinking water)은 무제한 공급하며 사육하였다. 이어, 실험동물 피부 중, 견갑하각(inferior border of scapular)에서 수평으로 5cm 이동한 부위에 위치한 2 ×2cm 넓이의 피부를 수술부위로 지정하고, 수술부위를 소독한 다음, 2％ 리도카인(lidocaine)을 사용하여 국소 마취하고, 수술부위의 진피를 포함한 피부조직을 절개한 후, 압박술로 지혈하고, 상처 부위를 소독하였다. 이어, 실시예1에서 제조된 가축 사료용 첨가제를 수술 2일째부터 상처 부위에 0.025g/cm2 양으로 도포한 실험군과 포타딘을 이용하여 소독치료만을 수행한 대조군을 제작하고, 대조군과 실험군에 있어서 초기 창상면적에 대하여, 경과 일수에 따른 창상면적의 비율을 구하여 창상잔존율(％)을 측정하였다. 그 결과는 표 1에 나타난 바와 같이, 실시예 1의 첨가제를 처리한 경우에는 초기 피부재생 반응이 빠르게 진행되므로 창상 잔존율이 대조군에 비해 신속하게 감소하는 것을 확인할 수 있었다.A healthy male New Zealand white rabbit (American Animal Breeding Center), 8 weeks of age (2 kg body weight), was raised with unlimited feed (drinking feed) and drinking water under breeding conditions. Subsequently, the skin of a 2 × 2 cm area located 5 cm horizontally from the inferior border of scapular in the experimental animal skin was designated as a surgical site, the surgical site was sterilized, and then 2% lidocaine was used. Local anesthesia was used, the skin tissue including the dermis of the surgical site was incised, hemostasis was performed by compression surgery, and the wound area was disinfected. Subsequently, a control group which was subjected to the disinfection treatment using the experimental group and the potadidine, which was applied with the additive for animal feed prepared in Example 1 in the amount of 0.025 g / cm 2 to the wound site from the second day, was prepared, and in the control group and the experimental group With respect to the initial wound area, the ratio of the wound area to the number of days passed was determined to determine the wound residual rate (%). As a result, as shown in Table 1, when the additive of Example 1 treated the initial skin regeneration reaction was confirmed that the wound residual rate is reduced rapidly compared to the control.

시간의 경과에 따른 창상잔존율의 변화 Changes in Wound Retention Rate Over Time 경과일수(일)Days Since Elapsed 대조군Control 실험군Experimental group 22 94 ± 294 ± 2 94 ± 394 ± 3 77 67 ± 567 ± 5 20 ± 520 ± 5 1515 40 ± 1040 ± 10 10 ± 310 ± 3

<실험예 2> 발굽 질환의 개선 효과 검토Experimental Example 2 Examination of Improvement Effect of Hoof Disease

발굽 질환의 하나인 발굽 종기에 감염된 가축에 대한 실시예 1에서 제조된 가축 사료용 첨가제의 개선효과를 검토하기 위해 소를 이용하여 필드에서 실험하였다. 여러 수준의 심각도로 발굽 종기에 걸린 66마리의 소 전체를 3군 즉, 양성 대조군인 옥시테트라시클린 투여군, 음성 대조군인 물 투여군 및 실시예 1의 첨가제 투여군으로 나누어 실험하였다. 각 군별로 치료를 시작하기 전에 각 소에 대한 손상 크기, 손상 위치, 손상 모양, 피부염 및 통증에 대해 평가하였다. 상기 물질들을 첫째주 동안 경구투여하였고, 2주째의 월요일, 수요일 및 금요일 각각에 일회 경구투여하였다. 2주 동안의 치료 동안 및 그 후 약 2주 동안 각 소를 조사하고, 상기 요소에 대해 평가하였다. 이어서, 손상 개선 백분율을 손상 크기의 평가를 기준으로 측정하였고, 양의 값의 손상 백분율은 개선됨을 나타내고, 높은 양의 값의 변화율일수록 낮은 양의 값의 변화율 보다 크게 개선되었음을 나타낸다. 그 결과는, 하기 표 1 및 표 2와 같이 실시예 1의 첨가제가 항생제인 옥시테트라시클린과 유사한 발굽 질환 치료효과를 나타내는 것으로 확인되었다.In order to examine the improvement effect of the additives for livestock feed prepared in Example 1 for the livestock infected with the hoof boil, which is one of the hoof diseases, the experiment was conducted in the field using cattle. All 66 cows with hoof boils with varying degrees of severity were divided into three groups: the oxytetracycline administration group, the negative control group, the water administration group, and the additive administration group of Example 1. In each group, the size of the injury, the location of the injury, the shape of the injury, the dermatitis and the pain for each cow were evaluated before starting treatment. The materials were orally administered during the first week and once orally each on Monday, Wednesday and Friday of the second week. Each cow was examined during the two weeks of treatment and for about two weeks thereafter and evaluated for these elements. The percentage of improvement in damage was then measured based on the assessment of damage size, indicating that the percentage of damage of positive values improved, and that the higher the rate of change of the value, the greater the rate of change of the lower amount of value. As a result, as shown in Table 1 and Table 2, it was confirmed that the additive of Example 1 exhibited a hoof disease treatment effect similar to that of oxytetracycline, which is an antibiotic.

2주 평가2-week evaluation 투여군Administration group 소의 숫자Number of cows 통증이 없음No pain 통증이 있음Pain 민감함Sensitive 손상 변화율Damage rate (1) 실시예 1의 첨가제(1) Additive of Example 1 2222 19(86 ％)19 (86%) 00 3(14 ％)3 (14%) +68 ％+68% (2) 옥시테트라시클린(2) oxytetracycline 2222 20(91 ％)20 (91%) 00 2(9 ％)2 (9%) +67 ％+67% (3) 물(3) water 2222 00 22(100 ％)22 (100%) 00 -15 ％-15%

30일 평가 30 day evaluation 투여군Administration group 소의 숫자Number of cows 통증이 없음No pain 통증이 있음Pain 민감함Sensitive 손상 변화율Damage rate (1) 실시예 1의 조성물(1) the composition of Example 1 2222 16(73 ％)16 (73%) 1(5 ％)1 (5%) 5(22 ％)5 (22%) +74 ％+ 74% (2)옥시테트라시클린 (25 mg/㎖)(2) oxytetracycline (25 mg / ml) 2222 17(77 ％)17 (77%) 4(18 ％)4 (18%) 1(5 ％)1 (5%) +68 ％+68% (3) 물(3) water 2222 00 22(100 ％)22 (100%) 00 -18 ％-18%

<실험예 3> 랫트에 대한 경구투여 급성 독성실험Experimental Example 3 Oral Acute Toxicity in Rats

6주령의 특정병원체부재(specific pathogen-free, SPF) SD계 랫트를 사용하여 급성독성실험을 실시하였다. 군당 5 마리씩의 랫트에 본 발명에 따른 실시예 1의 첨가제를 각각 0.5％ 메틸셀룰로즈 용액에 현탁하여 각각 20 g/㎏, 30 g/㎏ 및 50 g/㎏의 용량으로 1회 단회 경구투여 하였다. 투여 후 동물의 폐사여부, 임상증상, 체중변화를 관찰하고 혈액학적 검사와 혈액생화학적 검사를 실시하였으며, 부검하여 육안으로 복강장기와 흉강장기의 이상여부를 관찰하였다.Acute toxicity test was performed using 6-week-old specific pathogen-free (SPF) SD rats. Five rats per group were suspended orally in single doses of 20 g / kg, 30 g / kg and 50 g / kg, respectively, with the additive of Example 1 according to the invention suspended in 0.5% methylcellulose solution. After administration, mortality, clinical symptoms, and changes in body weight were observed, and hematological and blood biochemical tests were performed. Necropsy was performed to observe abdominal and thoracic organ abnormalities.

그 결과, 실시예 1의 첨가제를 투여한 모든 동물에서 특기할 만한 임상증상이나 폐사된 동물은 없었으며, 체중변화, 혈액검사, 혈액생화학 검사, 부검소견 등에서도 독성변화는 관찰되지 않았다. 이상의 결과, 본 발명의 가축 사료용 첨가제는 랫트에서 50 g/㎏까지 독성변화를 나타내지 않으며 따라서, 경구투여 중간치사량(LD50)은 50 g/kg 이상인 안전한 물질로 판단되었다.As a result, no significant clinical symptoms or dead animals were noted in all animals to which the additive of Example 1 was administered, and no toxic changes were observed in weight changes, blood tests, blood biochemical tests, autopsy findings, and the like. As a result, the additive for livestock feed of the present invention does not show a toxic change up to 50 g / kg in rats, and therefore, the oral administration intermediate dose (LD 50 ) was determined to be a safe substance of 50 g / kg or more.

상기에서 살펴본 바와 같이, 본 발명의 가축 사료용 첨가제는 표피세포를 재생시키고 가축발굽을 건강하게 하며 가축 사료의 효율을 증가시키므로, 가축의 피부질환 또는 발굽질환의 예방이나 치료에 유용하게 사용될 수 있다. As described above, the additive for livestock feed of the present invention regenerates the epidermal cells, makes the livestock hooves healthy and increases the efficiency of the livestock feed, and thus may be usefully used for the prevention or treatment of skin diseases or hoof diseases of livestock.

Claims (6)

리조푸스 0.05 내지 5.0 중량부, 비오틴 0.01 내지 1.0 중량부, 아연 0.2 내지 20 중량부, 크롬 0.006 내지 0.6 중량부, 구리 0.021 내지 2.1 중량부 및 코발트 0.001 내지 0.05 중량부로 구성되는 가축 사료용 첨가제.An additive for livestock feed comprising 0.05 to 5.0 parts by weight of lyfupus, 0.01 to 1.0 parts by weight of biotin, 0.2 to 20 parts by weight of zinc, 0.006 to 0.6 parts by weight of chromium, 0.021 to 2.1 parts by weight of copper and 0.001 to 0.05 parts by weight of cobalt. 제 1항에 있어서, 상기 첨가제는 리조푸스 0.08 내지 3.0 중량부, 비오틴 0.05 내지 0.5 중량부, 아연 0.5 내지 10 중량부, 크롬 0.01 내지 0.3 중량부, 구리 0.05 내지 1.5 중량부 및 코발트 0.001 내지 0.02 중량부로 구성되는 가축 사료용 첨가제The method of claim 1, wherein the additive is 0.08 to 3.0 parts by weight of lyfupus, 0.05 to 0.5 parts by weight of biotin, 0.5 to 10 parts by weight of zinc, 0.01 to 0.3 parts by weight of chromium, 0.05 to 1.5 parts by weight of copper and 0.001 to 0.02 parts by weight of cobalt. Additives for livestock feed 제 1항 또는 제 2항에 있어서, 상기 첨가제는 피부재생 효과를 가지는 것을 특징으로 하는 가축 사료용 첨가제.The additive for livestock feed according to claim 1 or 2, wherein the additive has a skin regeneration effect. 제 1항 또는 제 2항에 있어서, 상기 첨가제는 동물발굽 질환 치료 또는 예방 효과를 가지는 것을 특징으로 하는 가축 사료용 첨가제.The additive for livestock feed according to claim 1 or 2, wherein the additive has an effect of treating or preventing animal hoof disease. 제 1항 또는 제 2항에 있어서, 상기 첨가제는 운동능력 향상 효과를 가지는 것을 특징으로 하는 가축 사료용 첨가제.The additive for livestock feed according to claim 1 or 2, wherein the additive has an effect of improving athletic performance. 제 1항 또는 제 2항에 있어서, 상기 가축은 소 또는 말인 것을 특징으로 하는 가축 사료용 첨가제.The additive for livestock feed according to claim 1 or 2, wherein the livestock is cow or horse.
KR1020030084518A 2003-11-26 2003-11-26 A feed additive for a livestock KR20050050869A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105497072A (en) * 2015-11-05 2016-04-20 吉林农业大学 Pig cleft hoof disease prevention and treatment medicine blanket

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105497072A (en) * 2015-11-05 2016-04-20 吉林农业大学 Pig cleft hoof disease prevention and treatment medicine blanket
CN105497072B (en) * 2015-11-05 2020-03-13 吉林农业大学 Medicine blanket for preventing and treating pig split hoof disease

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