KR20050036928A - A health care composition for treating or preventing intestinal disease and constipation - Google Patents

Abstract

The present invention relates to a variety of herbal compositions containing essentially radish ( Raphanus sativus L.) and tea ( Camellia sinensis ), inducing intestinal function activity in animal models using charcoal and constipation-induced experimental models, By activating the mucous secretion of the intestine and showing an excellent effect on improving constipation, it provides an effective and safe health functional food for improving intestinal function and preventing and improving constipation disease.

Description

A health care composition for treating or preventing intestinal disease and constipation

     The present invention relates to a combination composition of several herbal medicines effective for improving intestinal function and preventing and treating constipation diseases, and more particularly, extracts and concentrates several herbal medicines such as radish and tea at an appropriate ratio to extract and concentrate bowel function and constipation. The present invention relates to a herbal extract having an effect of improving disease and a composition containing the same in an appropriate combination ratio.

In recent years, diets have increased intake of high-calorie, high-protein, meat-containing, high-calorie foods such as meat and instant foods, while reducing the intake of foods containing vegetables and fiber. It is well known that obese patients are on the rise.

Constipation has long been known as the source of all kinds of illnesses, and the appetite is not always present and the abdomen is bloated, and the excreted toxins of unexcreted stools are absorbed into the intestine, which is absorbed into the blood to promote skin aging, headaches, acne, and skin rashes. If constipation is severe, it causes hemorrhoids such as breakage of dentition and escape of hemorrhoids during defecation, and even colon cancer. As a result, constipation occurs in a country with a high cultural level. In particular, women and office workers suffer a lot from constipation.

Constipation (constipation) is less than twice a week to see if the stool is less than 35g stool is due to lack of exercise or stress accumulates bowel movements, the ability to move the stool is reduced. Constipation refers to a case in which the stool remains in the colon for more than a normal time. The stool is usually excreted 12 to 72 hours after the food is eaten, and the type of food ingested affects the bowel time. In other words, high fiber foods absorb liquid in the intestine to increase volume, make excreta, and excrete, whereas low fiber content is the opposite. Constipation itself is not a disease but a symptom of headache, vision disorders and allergies caused by toxic gases (hydrogen sulfide, nitrosoamine, ammonia, phenol) due to abnormal intestinal fermentation caused by constipation Active prevention and treatment are needed because it causes various serious secondary diseases such as diseases (depression), skin diseases (acne, etc.), bad breath, colon diseases (hemorrhoids, colon cancer, etc.), arteriosclerosis, high blood pressure, stroke, and immunodeficiency. Do. In order to improve constipation and smooth bowel function, dietary fiber, water intake, exercise, etc. have been attempted as the basis for the treatment of all constipation. Therefore, it causes various side effects. In particular, in the case of irritation laxative effect is gradually reduced during long-term use, moreover, when the long-term administration of the intestinal muscle plexus is destroyed "cognate type colon" may be lost colon function.

     Although herbal medicines and herbal medicines are generally recognized as having no side effects, most of the herbal laxatives currently used are those containing anthraquinone derivatives such as senna and rhubarb, which are irritant laxatives and are taken daily or especially during pregnancy. Taking is contraindicated.

     The most common functional foods for relieving constipation are dietary fiber, and various foods including kelp, yacon, three hundred scents, unidentified tea, Donggyuja tea, aloe, and algae are on sale, but have they been sufficiently proven scientifically? Is being questioned.

Various methods have been studied to solve the above problems, and as a result, various medicines or foods have been developed. For example, in order to solve the problems such as constipation can be seen as the priority of the activity of the intestinal function, many studies have been carried out for this.

For example, Korean Patent Publication No. 180452 discloses a health nutrition composition having an effect of constipation and odor improvement, wherein aloe aboresense powder is extracted from alcohol and an active ingredient of copper and three hundred s. The health nutrition composition which mix | blended the extract which extracted the active ingredient or its powder is described.

In addition, Korean Laid-Open Patent Publication No. 99-84271 discloses 1 from the group consisting of Donggyuja, Donggyu cotyledon, crystalline, cotyledopia cotyledons, presbytery, rhubarb, forget-me-not, senna leaf, mazain, supervised, daepok, wonhwa, pardu, black shaft, etc. Tea prepared by mixing more than one species is described, and Korean Patent Publication No. 149389 describes a herbal composition for treating constipation using dried tangerine peel, ginseng, Schisandra chinensis, alum, licorice, apricot seed and wolfberry. .

As such, many studies have been conducted to solve constipation using natural plants, and the present inventors have also focused on researching herbal medicines for improving intestinal function and constipation.

Radish ( Raphanus sativus L.) and turnip ( Brassica Lap.) Used in the present invention belong to Cruciferae and the sugars contained in the root are mainly glucose and fructose, in addition to kumaric acid, caffeic acid and ferulic acid. (ferulic acid), phenylpyruvic acid, gentidin acid, hydroxy benzoic acid and many amino acids. Alcohol extracts of radish roots have been reported to have antimicrobial and antifungal effects (Bo Bo-seop and Shin, Min-kyo; Hyangjedae, pp582-584, Younglimsa, 1998).

Camellia sinensis belongs to theaceae, and the young and soft leaves of the tea tree mainly contain purine alkaloids and caffeine, and the content is 1 to 5%. In addition to the trace amount, theobromine (theobromine), theophylline (theophylline) and xanthine (xanthine) is contained. Tannin in tea leaves is mainly galloyl - ℓ -, and catechol (galloyl- l -epigallocatechol) with epi-go, which is contained together with caffeine. The pharmacological action of tea leaves is mainly caused by xanthine derivatives (caffeine and theophylline), and also contains a large amount of tannic acid, thus acting as antibacterial and vitamin P. Pharmacological action is to excite the central nervous system, promote mental function, increase thinking ability, eliminate fatigue, expand coronary blood vessels, and diuretic (Jung Bo-seop and Shin, Min-Kyo; . The young leaves of the tea tree can produce various kinds of teas with different flavors depending on the processing method. For example, according to the manufacturing methods such as post-fermentation, fermentation, semi-fermentation, and non-fermentation, respectively, tea, black tea, oolong tea, Iron ore, cannon tea, green tea, etc. can be manufactured.

Carrots ( Daucus carota L. var. Sativa DC.) Belong to Umbelliferae, and the roots of carrots include α-, β-, γ-, ε-carotene, lycopene and phytofluene. It contains many carotenoids, vitamins B1, B2 and anthocyanidins. Its pharmacological effect is effective in treating dryness and it is effective in treating indigestion (Jung Bo-seop and Shin Min-kyo;

The dermis (陣皮, Aurantii nobilis Pericapium) will dry off the rind of the mature fruits of the tangerine trees (Citrus unshiu MARCOR.) Of the Rutaceae (Rutaceae), cheongpi (Aurantii immaturi Pericapium) is a peel of immature fruits of Rutaceae Tangerine Trees It contains dried essential oils such as limonene, flavonoid glycosides, hesperidin and citric acid, naringin, and aspartic acid. , Jinhae, expectorant, copulation drug, anti-type I allergic action (Bo Bo-seop and Shin Min-kyo; Hyang-Yaksa Dictionary, pp783-784, Yeonglimsa, 1998).

Fig ( Ficus carica L.) is a dry fruit of the fig tree of Moraceae, the main ingredient of which contains about 10% of sugars such as glucose and fructose and has a strong sweetness. Organic acids include malic acid and citric acid, as well as benzaldehyde and protease, a protein that is effective in treating cancer, and other enzymes such as lipase, amylase, and oxidase, as well as fiber and protein. Pharmacological action is used for lack of milk, loss of appetite, etc., has the effect of detoxification. The green leaves are applied to hemorrhoids, and the dry juice is used as a medicinal insecticide, and the dried leaves are used as a bath bath for neuralgia (Jung Bo-seop and Shin, Min-Kyo;

Onium ( Allium cepa L.) belongs to the family Liliaceae, with special tinge such as thiol, dimethyl disulfide, diallyl-disulfide and diallyl thioether, thiosulfine acid salt It contains a substance (i) and a small amount of malate. Roots, apertures, and leaves contain coumaric acid, caffeic acid, ferulic acid, and sinapic acid. Onion suppresses the rise of plasma cholesterol, lowers fibrinolytic activity, and can be used for atherosclerosis. It is effective for gastric apathy and enteritis. It has a bactericidal effect and is used as a diuretic and expectorant in folk medicine. ; Hyangjedae Dictionary, pp155-156, Younglimsa, 1998).

Mume Fructus is dried immature fruit of the Prunus mume of the Amygdalaceae. The fruit contains substances such as citric acid, malic acid, fluoride, cytosterol, and oleanolic acid. When immature, it contains clearing. It has antibacterial and antifungal action, and it is an alkaline food for fatigue recovery and constitution improvement. Especially, it has excellent detoxification effect and helps treat stomach upset and food poisoning. Sour taste secretes gastric juice and normalizes digestive system to digest Eliminates insufficiency and gastrointestinal disorders (Bo Bo-seop and Shin Min-kyo; Hyang-Yaksa Dictionary, pp628-629, Younglimsa, 1998).

Apricot tree ( Prunus armeniaca L.) belongs to Prunus armeniaca (Amygdalaceae), fruit apricot (haengja) contains citric acid, β-carotene, essential oils such as myrcene (myrcene), limonene, p-cymene cymene, terpinolene, and the like. It has the effect of antitussive and expectorant, so it is used for relieving, asthma, bronchitis and acute hepatitis in the private sector, and it is also good for skin beauty and is effective in removing freckles and blemishes. In oriental medicine, the seeds are used as a medicine called algae (杏仁), which is effective for fever, Jinhae, expectoration, and swelling, so it is used for coughing, asthma, bronchitis, sore throat, acute pneumonia and constipation. do. It is distributed in Korea, Japan, China, Mongolia, the United States, and Europe (Bo Bo-seop and Shin Min-kyo); Hyangje Ambassador, pp625-627, Yeonglimsa, 1998).

Anti-cancer activity of radish in the above herbal medicines ( Adv. Exp. Med. Biol. , 289 , pp153-163, 1991: Am . J. Epidermiol. , 144 , pp1015-1025, 1996) and liver detoxification ( Biosci. Biotechnol. Biochem. , 59 , pp1882-1886, 1995: Arch. Biochem. Biophys. , 316 , pp797-802, 1995) are known in the United States and Japan, and in particular, radish extract has been used in various functional foods in various ways and compositions. It has been used as a mixture, and various inventions have been proposed for use in health drinks. However, the health beverage composition using such a radish extract is usually only a method used as a beverage composition in vague expectation depending on the digestive function of the radish extract.

As a result of the present inventors focusing on research on foods that can prevent or treat constipation by activating intestinal function, it was confirmed that the extract consisting of several natural herbal medicines and their proper composition ratio has an excellent improvement effect, thereby completing the present invention.

The present invention provides a health functional food for improving intestinal function and preventing and improving constipation disease, comprising several herbal compositions comprising herbal extracts having intestinal function activity and appropriate combination composition thereof.

       In order to achieve the above object, the present invention provides a health functional food for improving constipation and intestinal function, including a herbal composition comprising a radish and tea extract and food supplements that can be added to the food.

Herbal extracts for improving bowel function and preventing and improving constipation diseases of the present invention is characterized in that the extraction with water or alcoholic aqueous solution, more specifically,

About 5 to 20 times after washing and drying in a dryer to remove foreign substances present on the surface of individual ingredients such as radish, tea, carrot, dermis, green skin, fig, onion, buckwheat and apricot, dried or undried Preferably, 10 to 12 times the amount of water or lower alcohol is added to the hot water extraction for 1 to 5 hours, preferably 2 to 3 hours at an extraction temperature of 70 to 120 ℃, preferably 90 ℃ or more, lyophilization, vacuum drying Preferably, the first step is a drying step of the individual material extract prepared by the step of making a powder extract by lyophilization;

After filtering the extract obtained from the extraction step, it was concentrated under reduced pressure at 40 to 80 ℃ X extract obtained by appropriate mixing ratio, preferably 1 to 2: 1 to 2: 0.1 to 1: 0.01 to 1: 0.01 to 1: 0.01-1: 0.1-2: 0.01-1: 0.01-1, or mixed with other suitable herbal medicines in an appropriate ratio, and then 5-20 times, preferably 10-15 times the volume of these complex herbal medicines. A lower alcohol such as distilled water, methanol, ethanol or the like or a mixed solvent thereof, preferably distilled water alone or a mixed solvent of 10 to 50% water and ethanol, and an extraction temperature of 70 to 120 ° C., preferably 90 ° C. or higher 1 to 5 hours, preferably 2 to 3 hours by hot water extraction through freeze-drying, vacuum drying, preferably lyophilized through a second step of drying the compound material extract prepared through the step of making powder extract To be obtained There.

In addition, the present invention for improving constipation and bowel function containing one or more extracts selected from carrots, dermis, green skin, figs, onions, ume or apricot extract in addition to the essential extracts Provide health functional food.

Preferred compositions of the health functional food of the present invention include radish, tea, carrot, dermis, green skin, fig, onion and ume extract; Radish, tea, carrot, dermis, green skin and onion extracts; Radish, tea, carrot, dermis and dermis extracts; It is a functional food containing radish, tea, carrot, dermis, dermis, fig and ume extract, even more preferably radish, tea, carrot, dermis, dermis, fig, onion, ume and apricot extract.

In addition, the present invention is a combination ratio of radish, tea, carrots, dermis, green skin, fig, onion, buckwheat and apricot extract is 1-2: 1-2: 1: 0.01-1: 0.01-1: 0.01-1: 0.01-1: 0.1 -2: 0.01-1: It is providing the health functional food which is 0.01-1.

The amount of the herbal composition of the present invention provides a health functional food having a weight ratio of 0.01 to 80%, preferably 1 to 50% of the total composition.

In addition, the present invention provides a product for improving intestinal function and constipation, containing a herbal composition essentially containing radish and tea and an acceptable food additive.

The product of the present invention is 10 to 80% by weight, tea 10 to 80% by weight, carrot 10 to 80% by weight, dermis 0.1 to 20% by weight, skin peel 0.1 to 20% by weight, fig 5 to 50% by weight, onion 5 It provides a product containing -50% by weight, 0.5-10% by weight, 1-50% by weight apricot extract, 5-30% oligosaccharides and 5-30% dietary components.

Preferably, radish extract 5-30%, tea extract 10-30%, carrot extract 10-20%, dermis extract 5-10%, green skin extract 5-10%, fig extract 10-20%, onion extract 10 ~ 20%, 5 ~ 10% omega extract, 10 ~ 20% apricot extract, 10 ~ 20% oligosaccharide and 10 ~ 20% dietary ingredient.

The compounding composition according to the present invention is characterized by showing an synergistic effect of the active ingredient formulated by the following composition ratio than the sole use for the purpose of improving bowel function and improving constipation.

The composition essentially comprising the radish and tea of the present invention can be used in a variety of food and beverages for the prevention of constipation and bowel disease.

Examples of foods to which the herbal composition comprising essentially the radish and tea of the present invention may be added include, for example, various foods, beverages, breads, cookies, sachets, candy, gum, tea, vitamin complexes, health functional foods, and the like. There is this.

The composition comprising the radish, tea extract and carrot, dermis, green skin, fig, onion, buckwheat or apricot extract of the present invention, the herbal medicine itself is used as a food, there is little toxicity and side effects, so it is safe even when taken for a long time for the purpose of prevention. Can be used.

The herbal medicine of the present invention may be added to food or beverage for the purpose of improving constipation and improving intestinal function. At this time, the amount of the herbal extract in the food or beverage is generally added to the health functional food composition of the present invention to 0.01 to 80% by weight of the total food weight, the health beverage composition is 0.02 to 30g based on 100ml, preferably Preferably at a rate of 0.3 to 5 g.

The health beverage composition of the present invention has no particular limitation on the liquid component except for containing the herbal medicine as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates, etc. as additional ingredients, like ordinary drinks. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And sugars such as conventional sugars such as polysaccharides such as dextrin, cyclodextrin and the like and xylitol, sorbitol, erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (e.g., Rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of said natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), dietary ingredients, flavoring agents such as synthetic and natural flavoring agents, colorants and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, Alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. The compositions of the present invention may also contain pulp for the production of natural fruit juices and fruit juice beverages and vegetable beverages. The present invention can be used as a mixed agent such as lactic acid bacteria preparation beverages or pastes such as yogurt. These components can be used independently or in combination.

The present invention provides a health functional food containing 0.01 to 30% by weight of the composition of vitamins, oligosaccharides, and dietary ingredients as a food supplement.

The proportion of such additives is not so critical but is generally selected in the range of 0 to about 30 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, the present invention will be described in detail with reference to the following Examples and Experimental Examples, but the present invention is not limited thereto.

Example 1. Preparation of Radish Extract

After cutting radish (Hungryong seedling) and drying it, 100 g of 100 g of water was added 1 l of water, and extracted for 2 hours at 100 ° C. The extract was concentrated under reduced pressure using a vacuum condenser (Buchi R020, Germany) and freeze-dried. , Or radish was finely squeezed or radish was concentrated under reduced pressure as described above, and then lyophilized radish extract was used as a sample.

Example 2. Preparation of Tea Extract

Green tea (Shinheung Dawon, Hwagae), Oolong tea (Taiwan), Iron Gwaneum (China), Boy tea (China) and Black tea (Sri Lanka) were purchased and used as samples.

Example 3. Preparation of Carrot Extract

Washed and dried carrots (heungjong seedlings) 100g or raw carrots were cut or obtained by performing the same method as in Example 1, the juice was used as a sample.

Example 4. Preparation of Dermis Extract

100 g of dried dermis (Gyeongdong Market) was obtained in the same manner as in Example 1, and used as a sample.

Example 5. Preparation of Peel Extract

100 g of dried Cheongpi (Gyeongdong Market) was obtained by performing the same method as Example 1, which was used as a sample.

Example 6 Preparation of Fig Extract

100 g of dried figs (Samho Agricultural Cooperative) were obtained by the same method as Example 1, and used as a sample.

Example 7. Preparation of Onion Extract

100 g of onion (heungjong seedlings) or raw onion was cut or obtained by performing the same method as in Example 1 by taking out juice, which was used as a sample.

Example 8. Preparation of Ome Extract

100 g of ume (Gyeongdong Market) was obtained by the same method as Example 1, and used as a sample.

Example 9 Preparation of Apricot Extract

100 g of dried apricots (Samho Agricultural Cooperative) was obtained by the same method as Example 1, and used as a sample.

Example 10 Preparation of Herbal Composition I

Of the herbal extracts prepared in Examples 1 to 9, 20 times purified water was added by combining radish, green tea, carrot, dermis, green skin, fig, onion, and ume in the weight ratio as shown in Table 1 below, and then, at 100 ° C. The extract obtained by time extraction was concentrated under reduced pressure and freeze-dried to use it as a sample.

group Herbal medicine Composition ratio (%) I radish 30 green tea 10 carrot 20 dermis 5 Cheongpi 10 FIG 10 onion 10 Hawk 5

Example 11 Preparation of Herbal Composition II

Of the herbal extracts prepared in Examples 1 to 9, radish, oolong tea, carrot, dermis, green skin, fig, onion, ume, and apricot were added to 20 times purified water by combining in a weight ratio as shown in Table 2 below. The extract obtained by extraction at 2 ° C. was concentrated under reduced pressure and freeze-dried to use it as a sample.

group Herbal medicine Composition ratio (%) Ⅱ radish 20 Oolong tea 25 carrot 10 dermis 10 Cheongpi 10 FIG 10 onion 10 Hawk 5 apricot 10

Example 12 Preparation of Herbal Composition III

Of the herbal extracts prepared in Examples 1 to 9, radish, black tea, carrot, dermis, green skin, onion was added by combining 20 times the purified water by weight composition ratio as shown in Table 3 below and obtained by extracting at 100 ℃ for 2 hours The extract was concentrated under reduced pressure and freeze-dried to use it as a sample.

Formulation Herbal medicine Composition ratio (%) Ⅲ radish 10 black tea 20 carrot 20 dermis 20 Cheongpi 20 onion 20

Example 13 Preparation of Herbal Composition IV

Radish, carrot, tea (iron guanyin), dermis, cheongpi was combined in the weight ratio as shown in Table 4 below, radish, carrot was obtained by extracting juice and added to the remaining herbal medicine 10 times purified water and extracted at 100 ℃ for 2 hours The extract was concentrated under reduced pressure and freeze-dried to use it as a sample.

group Herbal medicine Composition ratio (%) Ⅳ radish 30 Tea (iron Kannon) 20 carrot 30 dermis 10 Cheongpi 10

Example 14 Preparation of Herbal Composition V

Combining radish, black tea, carrot, dermis, green skin, fig, and ume with the weight composition as shown in Table 5, 20 times purified water was added, and the extract obtained by extracting at 100 ° C. for 2 hours was concentrated under reduced pressure and freeze-dried. Used as.

group Herbal medicine Composition ratio (%) Ⅴ radish 10 black tea 10 carrot 30 dermis 5 Cheongpi 2 FIG 30 Hawk 3

Example 15 Preparation of Herbal Composition VI

The radish, onion and carrots are concentrated in the composition shown in Table 6, juice, added to the combined weight ratio of the rest of the herbal medicines, added 5 times purified water and extracted for 2 hours at 100 ℃ concentrated and freeze It was dried and used as a sample.

group Herbal medicine Composition ratio (g) Ⅵ radish 20 Boycha 10 carrot 15 dermis 10 Cheongpi 10 FIG 10 onion 20 Hawk 5

Example 16 Preparation of Health Beverages

3000 mg of radish extract of Example 1, 3000 mg of tea extract of Example 2, 2000 mg of carrot extract of Example 3, 100 mg of dermis extract of Example 4, 100 mg of dermal extract of Example 5, fig of Example 6 Purified water was added to 2000 mg of the extract, 200 mg of the onion extract of Example 7, 50 mg of the mae extract of Example 8, 100 mg of the apricot extract of Example 9, 100 mg of citric acid, 100 g of oligosaccharide, and 1 g of the dietary ingredient, and a total volume of 900 ml. made. After mixing the above components in accordance with a conventional healthy beverage production method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution was filtered and obtained in a sterilized 2 L container, sealed sterilization and refrigerated.

Experimental Example 1.Effects on Activated Carbon Dietary Migration of Herbal Medicine Compositions

In order to evaluate the effect of improving bowel function and constipation, an activated charcoal meal transfer experiment was performed as follows.

Experimental animals were experimented with 8 animals per group divided into control group and test group, using male mice (Balb / C) male (average biolink, negative) having an average body weight of 24 to 27g. That is, the test group was dissolved in drinking water at 3.2 mg / ml concentration of the herbal composition prepared in Examples 10 to 15 and administered for 3 days, and then fasted at 18 o'clock on the fourth day. The herbal composition continued to be administered during fasting. The control group was treated in the same manner but administered only drinking water containing no herbal composition. Activated charcoal (8 mg (0.2 ml) orally administered to each herbal composition at 9 days on the 5th day and 60 minutes later, activated carbon (Sigma, USA) and Arabian gum (Sigma, USA) were mixed at a weight ratio of 5% and 10%, respectively. 0.2 ml of a charcoal meal was orally administered and sacrificed after 30 minutes to extract the gastrointestinal tract. Activated charcoal diet was investigated the rate of migration (R _f ) in the intestinal distance from duodenum to ileum. The same experimental method is called charcoal meal transit.

As a result, the intestinal migration effect of the activated carbon diet was significantly increased when the herbal composition of each group was administered as compared to the control group administered with only drinking water. As a result, it was found that each herbal composition has a significant bowel movement promoting effect, in particular, it was confirmed that the herbal composition II has an excellent activity compared to other herbal compositions (see Table 7).

Experimental group % Growth Improvement rank Control 100.0 - Herbal Composition I 143.7 3 Herbal Composition II 146.5 One  Herbal Medicine Composition III 142.9 4  Herbal Medicine Composition IV 138.8 5 Herbal Composition V 145.3 2  Herbal Medicine Composition VI 131.2 6

Experimental Example 2. Constipation improvement effect of each herbal composition

In order to determine the effect on the improvement of bowel function and constipation of each herbal composition group prepared in Examples 10 to 15 was carried out by performing the following method.

In order to measure bowel variability, animal models were purified for 3 days in metabolic cages using 8 to 10 Sprague Dawley rats (Dae Biolink, Negative) weighing between 220 and 240 g in males. A diet containing 1 mg per 3 g of loperamide (Sigma, USA) was fed to acclimate and cause constipation from day 4. The control group (roperamide group treatment group) was supplied with only drinking water, and the herbal composition was dissolved in drinking water at a concentration of 3.2 mg / ml and administered until the end of the experiment. Stools were taken daily and weighed.

As a result of the constipation improvement effect of each herbal composition, each test group was confirmed to have a constipation improvement effect by increasing the bowel movements during the constipation induction period compared to the control group, in particular, the herbal composition Ⅱ is excellent by increasing the variance during the constipation induction period It has been shown to improve constipation (see Table 8).

Experimental group Variable increase compared to control (%) Days of sample administration D + 1 D + 2 D + 3 D + 4 D + 5 D + 6 D + 7 D + 8 I 54 * 31 * 7 22 19 * 23 * 11 4 Ⅱ 44 * 64 ** 34 * 14 31 * 16 16 30 * Ⅲ 10 53 ** 28 * 21 One 2 10 12 Ⅳ 19 18 23 * 19 23 21 9 26 V 2 30 * 40 ** 42 * 24 23 51 9 Ⅵ 10 20 30 * 15 20 18 5 10  D: Day of administration of the sample containing loperamide, * p <0.05, ** p <0.01

Experimental Example 3. Constipation improvement effect of herbal composition Ⅱ

Among the various compositions, the herbal composition II showing the most excellent constipation relieving effect was investigated in the same way as the constipation induction method of Experimental Example 2, and the variation, moisture content, feed intake and water intake were investigated.

As a result of administering the herbal composition II, it was confirmed that the constipation improvement effect was improved by the increase in solid content of lyophilized stools compared to the control group, and thus the herbal composition II was relatively resolved the constipation symptoms, resulting in increased appetite, feed intake and water intake. It can be interpreted as (see Table 9).

Item % Increase compared to control Days of sample administration D + 1 D + 2 D + 3 D + 4 D + 5 D + 6 D + 7 D + 8 Variable 43.9 64.4 34.1 13.8 31.5 16.5 16.2 30.5 Dry variable - 14.1 25.7 28.3 16.8 18.9 38.4 2.3 Variable moisture content - 42.6 54.2 53.8 32.2 21.3 63.6 17.3 Feed intake 15.3 13.6 5.9 2.6 1.5 0 -0.6 3.9 Variable / Feed Intake 28.2 41.9 27.2 7.2 35.1 9.6 15.1 13.0 Water intake 32.2 35.7 11.9 30.5 24.8 15.7 -0.3 19.0  D: Day of administration of the feed containing loperamide,-: No measurement

Experimental Example 4. Effect of herbal composition II on secretion of mucus in the intestinal tract

To investigate the effect of improving the bowel function related to the constipation effect of herbal composition II, the effect on mucus secretion in the intestinal tract was investigated.

Mucus in the digestive system protects the intestinal surface, the epithelial tissue from mechanical damage and chemical irritation, and acts as a lubricant for digestive movement. The intestinal mucosa is covered with mucus, and mucin plays the most important role in indicating the physical and chemical properties of the mucosa. It is an alkaline to viscous fluid. In the case of constipation, the function of the mucosal layer in the intestinal mucosa is reduced, and administration of loperamide for a certain period of time thinning the thickness of the mucous membrane of the intestinal tract, which causes the movement of the contents of the colon.

     Treatment for the experiment was carried out in the same manner as in Experimental Example 2 above, and extracted from the caecum of the experimental animal to the rectum and fixed in 10% formaldehyde phosphate buffer solution to the naked eye to the number of feces pellet (feces pellet) in the colon Observed.

As a result, the average number of stools in the control group was 5.5, compared to 1.75 in the herbal composition II, which was 68.2% decreased (see Table 10). Composition II had almost no cecum and rectal residues, and the control had a lot of residual content (see FIG. 1). These results were consistent with the number of bowel movements and the number of bowel residues, confirming that the herbal composition II has an excellent anti-constipation effect.

In addition, as a result of observing the mucous secretion cells and the mucosa through histological examination to observe the secretion of mucosa in the intestinal tract, the herbal composition II administration group was activated mucus secretion cells than the control group to facilitate the secretion of mucus and the thickness of the mucus constipation. More markedly (see FIGS. 2A and 2B). The mucus thickness of the normal control group was 17.2 μm, while the constipation-inducing group had a thickness of 11.9 μm, which is about 31% lower than that of the normal control group, while the herbal composition II administration group was 17.4 μm, similar to the normal control group (Table 11). Reference and FIGS. 3A, 3B).

As a result, the herbal composition II was found to increase mucus to maintain intestinal lubrication and increase intestinal motility to alleviate constipation symptoms.

Test group Control Herbal composition II administration group The number of bowels 5.5 1.75

Test group Mucus thickness (μm) % Increase compared to normal control Normal control 17.2 ± 3.24 ¹ 100 Control 11.9 ± 2.49 ^** 69.2 Herbal composition II administration group 17.4 ± 5.55 ^$$ 101.2 1: mean ± standard deviation, **: p <0.01, $$: p <0.01 compared to control

Experimental Example 5. Toxicity Test

Animal experiments were conducted to confirm the toxicity of the herbal composition II prepared in Example 11.

After dividing the ICR mouse (25 ± 2g, the central laboratory animals) and the Sprague Dawley mouse (Sprague Dawley, 200 ± 20g, the central laboratory animals) into nine groups of 10 each, the herbal composition II of the present invention was 1000 And drinking water at a dose of 5000 mg / kg for 1, 2 or 3 months.

As a result of toxicity after oral administration, none of the nine groups died and no symptoms were apparent from the control group. In addition, hematological and biopsy findings were not specific.

Experimental Example 6. Human application test (preliminary clinical trial)

      Herbal composition II, which showed the best effect on improving constipation in the animal experimental model, was administered to 4 people with constipation disease once every 2g, for 1, 2 or 3 months.

As a result of the experiment, the constipation symptom was improved in all the patients, and confirmed the effect of improving bowel function and constipation in the human body (see Table 12).

gender Constipation Secondary symptoms How to take effect Female (23 years old) -1-2 times / week stool-Excessive force- Remnant -Severe acne-bowel discomfort -2 times / day for 1 month-1 times / day for 2 months 1-2 times / week for 3 months -3 days after administration, large amount of bowel movements-discoloration turns from black to brown-relaxation of stool hardness-relieves lingering and intestinal discomfort Female (36 years old) -1-2 times / week stool-Excessive force- Remnant -Intestinal discomfort-Anorexia -2 times / day for 1 month- 1 times / day for 2 months -Large amounts of bowel movement after 5 days of administration-Discoloration changes from black to brown-Relieves hardness of stools-Relieves lingering and intestinal discomfort Female (22 years old) -1-2 times / week stool-Excessive force- Remnant Difficulty in bowel movements -2 times / day for 1 month 1 / day for 2 months -3 days after bowel movement-Relieve constipation after taking 1 month-Stop taking constipation after 2 months Female (33 years old) -1-2 times / week stool-Excessive force- Remnant -Weak constipation effect-severe acne -3 times / day for 1 month-2 times / day for 2 months -The first 5 days of gas discharge-5 days after a large amount of bowel movements-1 day per bowel movement

The herbal extract of the present invention can be administered in the following formulations, and the following formulation examples are merely illustrative of the present invention, whereby the content of the present invention is not limited.

Formulation Example 1 Preparation of Suppositories

75 g of the herbal composition of Example 11

1 g of tetrahydrozoline hydrochloride

Lidocaine 60 g

5 g of hydrocortisone acetate

Allantoin 20 g

Tocopherol acetate 60 g

20 g of hard silicic anhydride

Permazole Bl15 1409 g

The components are sequentially dispersed in a stirred permazole B115 heated at 50 ° C. to 70 ° C. while stirring, cooled to about 40 ° C., and then filled into a suppository container and cooled to form a suppository.

Formulation Example 2 Preparation of Tablet

200 mg herbal composition of Example 11

Lactose 100 mg

Starch 100 mg

Magnesium stearate proper amount

A tablet is prepared by mixing and tableting the above components according to a conventional tablet manufacturing method.

Formulation Example 3 Preparation of Capsule

100 mg of the herbal composition of Example 11

Lactose 50 mg

Starch 50 mg

Talc 2 mg

Magnesium stearate appropriate amount

According to a conventional capsule preparation method, the above ingredients are mixed and filled into gelatin capsules to prepare capsules.

Formulation Example 4 Preparation of Liquid

1000 mg of herbal composition of Example 11

20 g of sugar

20 g of isomerized sugar

Lemon flavor

Purified water was added to make a total of 1000 ml. According to the conventional method for preparing a liquid, the above components are mixed, and then filled into a brown bottle and sterilized to prepare a liquid.

Formulation Example 5 Preparation of Health Functional Food

1000 mg of herbal composition of Example 11

20 g of vitamin mixture

70 μg of Vitamin A Acetate

Vitamin E 1 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

0.2 μg of vitamin B12

Vitamin C 10 mg

10 μg biotin

Nicotinic Acid 1.7 mg

Folate 50 ㎍

Calcium Pantothenate 0.5mg

Mineral mixture

Ferrous Sulfate 1.75 mg

Zinc Oxide 0.82mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium Citrate 90 mg

Calcium Carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the vitamin and mineral mixture is a composition suitable for a relatively healthy food in a preferred embodiment, the composition ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method, Granules may be prepared and used to prepare health food compositions according to conventional methods.

Formulation Example 6 Preparation of Health Beverage

1000 mg of herbal composition of Example 11

Citric acid 100 mg

100 g oligosaccharides

Add 900 ml of purified water

After mixing the above components in accordance with a conventional healthy beverage production method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and then refrigerated and stored in the present invention For the preparation of healthy beverage compositions.

Formulation Example 7 Bread Production

Herbal Composition 1 to 5g of Example 11

1 to 1.5 kg

Sugar 15-20 g

15-20 g of salt

Bread improver 9-15 g

Butter 40-50 g

Milk powder 35-45 g

Yeast 30-40 g

600-800 g of water

After mixing the above components, it is prepared according to the conventional bread production method.

Formulation Example 8 Cookie Preparation

5.9 ~ 11.7% herbal composition of Example 11

Sugar 12.5-16.7%

Margarine 23.3-25%

Egg 7.5%

Wheat flour 45%

After mixing said components, it manufactures according to a conventional cookie manufacturing method.

Formulation Example 9 Candy Preparation

5 g of herbal composition of Example 11

Maltitol 50g

44.5g reduced syrup

Oligosaccharide 0.2g

Grapefruit Seed Extract 0.1g

Natural Herbal Flavor 0.1g

The above raw materials can be blended to produce a conventional candy production method.

Formulation Example 10 Yogurt Preparation

10 parts by weight of the herbal extract of Example 11

12 parts by weight of skim milk powder

4 parts by weight of glucose

2 parts by weight of sucrose

1 part by weight of betacyclodextrin

70 parts by weight of water

After mixing and homogenizing the raw materials, heat-treated at 85 to 90 ℃ temperature for 10 minutes, mixed with 1 part by weight of lactic acid bacteria spawn to the cooled sample and incubated at 50 ℃, 8 hours to produce, produce bananas, strawberries according to your preference 8 parts by weight of jam or fruit preservation prepared with peaches, apricots, kiwis, and 0.07 parts by weight of yogurt flavors are mixed and homogenized to prepare a fermented product of the lake.

Although the composition ratio is a composition suitable for a preferred beverage in a preferred embodiment, the compounding ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and use purpose.

Radish and tea extract of the present invention and the herbal composition containing the same in an appropriate combination ratio not only promote intestinal migration effect, but also has an excellent intestinal mucus secretion promoting activity to treat bowel dysfunction and constipation disease and It can be used very effectively in health functional food for improvement.

Figure 1a is a picture of the colon extracted from the rat normal control, Figure 1b is a picture of the colon extracted from the rat treated with constipation-induced loperamide-induced constipation, Figure 1c is a loperamide and the present invention A photograph of the large intestine extracted from the rat to which the herbal composition II was administered,

FIG. 2a is a diagram illustrating the activity of mucin-secreting cells in rat colon tissues of the control group (loperamide-administered group), and FIG. 2b is a diagram of the activity of mucin-secreting cells in rat colon tissues of the herbal composition II administration group.

Figure 3a is a view of the mucous thickness of the rat colon tissue of the control group (loperamide administration group), Figure 3b is a diagram of the mucous membrane thickness of the rat colon tissue of the herbal composition II administration group.

Claims (8)

A health functional food for improving intestinal function and constipation, comprising a herbal composition comprising essentially radish and tea extracts and a food acceptable additive. The method of claim 1, further comprising carrot ( Daucus carota var sativa), dermis ( Aurantii nobilis Pericapium), green skin (A urantii immaturi Pericarpium), fig ( Ficus carica L.), onion ( Allium cepa L.), oume ( Mume Fructus) Or functional food for improving bowel function and constipation, comprising at least one extract selected from Prunus armeniaca extract. The health functional food according to claim 1 or 2, wherein the composition contains radish, tea, carrot, dermis, green skin, fig, onion, ume and apricot extract. According to claim 1 or 2, wherein the herbal composition of the radish, tea, carrots, dermis, green skin, figs, onions, ume and apricot extract in a blending ratio of 1 to 2: 1 to 2: 0.1 to 1: 0.01 ~ 1: 0.01-1: 0.01-1: 0.1-2: 0.01-1: 0.01-1 healthy functional food. The health functional food according to claim 1 or 2, wherein the amount of the herbal composition has a weight ratio of 0.01 to 80% of the total composition. The health food according to claim 1 or 2, wherein the health functional food is any one selected from bread, cookies, bread, candy, gum, beverage or yogurt. The health functional food according to claim 1 or 2, wherein the health functional food is a beverage. The extract according to claim 7, 5-30% radish extract, 10-30% tea extract, carrot extract 10-20%, dermis extract 5-10%, skin extract 5-10%, fig extract 10-20%, onion extract A functional beverage containing 10-20%, 5-10% ume extract, 10-20% apricot extract, 10-20% oligosaccharide and 10-20% dietary ingredient.