KR200445823Y1 - Split culture apparatus for microorganism - Google Patents

Abstract

The present invention relates to a two-part microbial incubator, which provides a culture tank, attaches an electric heater and a refrigerant pipe to an outer surface of the culture tank, and in the microbial incubator in which an exhaust fan is attached, the culture tank is divided into two. The aerobic and anaerobic microorganisms or photonutrients and inorganic nutrients can be partitioned on the machine room to simultaneously or selectively incubate, so that two or more different microorganisms can be cultured simultaneously in one microbial incubator. In one machine room, two temperature, circulation, light source, sterilization and aeration can be controlled.

Description

SPLIT CULTURE APPARATUS FOR MICROORGANISM}

The present invention is a machine for culturing microorganisms such as photosynthetic bacteria, lactic acid bacteria, Bacillus bacteria, and yeast bacteria, which are mainly used in agriculture, fisheries, and water purification, and related to a microbial incubator that provides purified air to a culture tank requiring aseptics. In particular, the present invention relates to a two-part microbial incubator that can provide a more efficient culture system by using a two-part type culture tank used for culturing microorganisms.

The present invention relates to a microbial culture apparatus, and more particularly, to a two-part microorganism culture apparatus capable of mass culture and excellent storage capacity by allowing two microorganisms to be cultured in one system.

Photosynthetic bacteria, lactic acid bacteria, Bacillus bacteria, yeast bacteria and cyanobacteria are microorganisms used in the fields of concentrated livestock, fisheries and water purification. When used in agriculture, they are used to treat seeds, improve soil, prevent contamination of various bacteria, or promote growth rate. When used for livestock farming, it can be applied to livestock feed or drinking water to purify manure, remove odors, inhibit the growth of pathogens, improve digestibility, and supply minerals.

When used in fisheries, water purification effect, decomposition and purification effect of the bottom part of the farm, inhibition of pathogen generation, improvement of disease resistance, role of feed of fry, etc. can be seen. It can also be used for the purification of fermentation agents and various industrial wastes.

The tank-type microbial incubator has been known in order to easily cultivate such microorganisms, but the conventional microbial incubator has a problem that it is difficult to cultivate a large capacity of more than 200ℓ and move well without handling. Therefore, as part of overcoming the limitations of the incubator, an acrylic resin plate, polyethylene resin plate, or PVC resin plate is attached to the inner surface of the metal container so that a large amount of microorganisms such as metal containers can be cultured in large quantities. One technology is disclosed in Korean Patent Application Publication No. 10-2006-0002082 (2006.01.09).

However, even if the microbial incubator with a PVC resin plate-attached strong stainless steel culture tank as described above has the limitation that only one microorganism can be cultured, it contains a disadvantage that it is impossible to culture a plurality of microorganisms.

In general, the microbial incubator 100 is provided with a single culture tank 110 of a container of a predetermined size and attaches an electric heater and a refrigerant pipe to the outer surface of the culture tank 110 and the exhaust fan (top) 150 is attached to the light source device 130 is installed.

In addition, the machine room 140 of the microbial incubator 100 includes an air attracting injector used to maintain cleanliness in hospitals, food factories, and indoor and underground living spaces, and an electric power air generator for attracting air to the injector. ), The steam generator 170, and the supply elements of the refrigerant pipe and the heating heater (not shown) are provided.

In addition, the culture tank (110) of one type of microorganism culture type has a problem such that the culture efficiency is reduced, such as having to use two microbial incubators 100 that are manufactured in a single form when culturing anaerobic or aerobic microorganisms. there was.

In addition, in the machine room 140 of each of the two microbial incubators 100, a heating heater and a refrigerant pipe supply element, an air attracting injector 200, and an electric power air generator 120 and a steam generator 170 are provided. Each of them has been ineffective aspects such as the power consumption and the space required for layout.

Therefore, for example, inefficient culture such as having to provide two incubators because two kinds of microorganisms (Vat with light source and Vat without light source) cannot cultivate a plurality of microorganisms at the same time in one incubator. There were disadvantages such as being forced to perform work.

Moreover, in the case of separately culturing two anaerobic and aerobic microorganisms, the cultivation is very effective by arranging two components for temperature, circulation, light source, aeration, etc. in two machine rooms, each of which is required for two microbial incubators. It would imply such a disadvantage that it cannot be realized.

In order to realize this, the present invention,

In the microbial incubator provided with a culture tank, the electric heater and the refrigerant pipe is attached to the outer surface of the culture tank, and the light source device container attached to the exhaust fan is attached to the upper side,

The culture tank is divided into two compartments characterized in that the compartment is arranged in the upper portion of the machine room to cultivate aerobic and anaerobic microorganisms simultaneously or selectively.

In addition, when cultivating photonutrients and inorganic nutrients, two different culture conditions (light vat, light-free vat) can simultaneously culture different microorganisms in a single system equipped with a two-part culture tank. With one machine room, you can control the temperature, circulation, light source, sterilization and aeration at once.

1 is a full perspective view of a conventional microbial incubator,

Figure 2 is a longitudinal cross-sectional structure of the microorganism incubator of Figure 1,

Figure 3 is a side cross-sectional view schematically showing the internal structure of the two-part microbial incubator according to the present invention, and

4 is an enlarged cross-sectional view illustrating an enlarged portion A of FIG. 3;

<Explanation of symbols for the main parts of the drawings>

   300: two-part microorganism incubator 311, 313: culture tank

   120: electric power air generator 150: exhaust window

   160: insulation 170: steam generator

   200: injector 140: machine room

   400: air distributor 410: first check valve

   411: first valve seat 413: first spring

   420: second check valve 421: first valve seat

   423: second spring 430: cam member

   431 depression

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Figure 3 is a side cross-sectional view schematically showing the internal structure of the two-part microbial incubator according to the present invention, Figure 4 is an enlarged cross-sectional view showing a portion A of FIG.

In the present invention, when cultivating photonutrients and inorganic nutrients, two culture conditions (lighting vat, light-free vat) can be cultured in one culture tank (311, 313) of different microorganisms simultaneously. As a two-part microorganism incubator 300 for controlling the temperature, circulation, light source, sterilization, aeration, etc. of the plurality of culture tanks (311, 313) to the machine room, the outer surface of the culture tanks (311, 313) An electric heater and a refrigerant pipe are attached thereto, and a light source device 130 having an exhaust fan attached thereto is attached to the culture tank 311 on the left side as an example.

In addition, the culture tanks (311, 313) is divided into two compartments are arranged on the machine room 140 to simultaneously or selectively culture aerobic and anaerobic microorganisms.

Of course, the inside of the culture tanks (311, 313), in particular, all parts in contact with the culture medium is based on the resin material does not occur rust, corrosion-resistant materials such as stainless steel can also be used.

In addition, between the two divided culture tanks (311, 313), there is a channel 403 for the air flow therein, the center of the power air generator (120, compressor, etc.) and the steam generator 170 An air distributor 400 having a body 401 having an inlet 405 for line connection is provided in a sealable manner.

In addition, the air distributor 400 has a recessed portion 431-equipped with a cam member (431) rotatably supported in association with the rotation shaft M10 of the drive motor M in the center of the channel 403 of the body 401 ( 430 is provided.

In addition, the inline type first check valve 410 and the second check valve 420 having the valve seats 411 and 421 and the springs 413 and 423 arranged side by side are opposite to both sides of the channel 403. The cam member 430 is interposed therebetween, and each of the cam members 430 is received and supported in a sealable manner via an O-ring (O).

In addition, reference numeral N denotes a nozzle portion N fastened to the front end of the check valves 410 and 420, and when the nozzle N is used, the air is entirely inside the culture tanks 311 and 313. Can be supplied quickly.

Next, the operation of the two-part microbial incubator according to the present invention will be described.

First, when the driving motor M is driven by applying power to the driving motor M of the air distributor 400, first and second check valves 410 and 420 of both sides according to the rotation angle of the rotation shaft M10. Simultaneously pressurizes the valve seats 411 and 421 of the cam member to simultaneously open the elastically supported valve seats 411 and 421 to simultaneously supply air, oxygen, or steam to both culture tanks 311 and 313. Done.

On the other hand, as can be seen in the enlarged partial view of the upper side of Figure 4, when the cam member 430 is rotated 90 degrees, the valve seat 421 of the second check valve 420 on the right side does not press Accordingly, only the valve seat 411 of the first check valve 410 is opened so that the air supply is started, so that it can be usefully coped with when selectively operating only the culture tank 311.

Thus, by installing an air distributor 400 that simultaneously spans the plurality of culture tanks (311, 313) of the two-part microorganism incubator 300 used in the culture of microorganisms, the air in each culture tank (311, 313) By taking the power air generator 120 and the steam generator 170 capable of selectively or simultaneously supplying oxygen or steam, it is possible to implement a more efficient microbial culture system.

In addition, the culture tank box (311, 313) of the present application to provide a clean and high-purity fermentation / culture material by preventing the mutual contamination of the culture medium by using the in-line type check valve so that the culture medium between the inflow back In line with the intent.

As described above, the present invention is capable of cultivating different microorganisms at the same time in two microbial incubators with two culture conditions (Vat with light source and Vat without light source). It is very effective in controlling sterilization and aeration.

As a result, material cost and manufacturing process cost reduction (consumer price reduction), multiple microorganisms simultaneous culture (reduction of culture time), and long-term storage are possible, and thus the washing and steam sterilization functions of each culture tank can be selectively added. Advantages will be given.

Although the present invention has been described with reference to the preferred embodiment shown in the drawings, this is merely exemplary, and those skilled in the art may various modifications and other equivalent embodiments therefrom.

Claims (3)

In the microbial incubator provided with a culture tank, The culture tanks (311, 313) is divided into two, partitioned on the machine room 140 to be selectively cultured aerobic microorganisms and anaerobic microorganisms in the two divided culture tanks (311, 313), Between the two-divided culture tanks 311 and 313, a channel 403 for introducing air into the two-divided culture tanks 311 and 313 is provided at the center thereof. The air splitter (400) having a body (401) having an inlet (405) for connecting the line with the power air generator (120) installed in communication) is installed between the two microbial incubator sealably. The method of claim 1, The light source device 130, the culture tank of one of the two divided culture tank (311, 313) to selectively incubate photonutrients and inorganic nutrition in the two-divided culture tank (311, 313) Microorganism incubator, characterized in that arranged in the upper and lower parts of (311). The method of claim 1, The air distributor 400 includes a depression 431-a cam member 430 that is rotatably supported in conjunction with a rotational axis of the driving motor M at a central portion of the channel 403 of the body 401. , On both sides of the channel 403, the inline type first check valve 410 and the second check valve 420, which are disposed in parallel with the valve seats 411 and 421, are disposed to face the cam member 430. The two-part microbial incubator, characterized in that each of the O-ring (O) is sealed to be received.