KR200417499Y1 - Adipose tissue regeneration kit - Google Patents

Abstract

The present invention relates to a kit for adipose tissue repair.

The present invention is a lock device for this purpose (Kit) at the bottom of the box, the sterile and sterilized adipose tissue washing solution is filled with a washing container 22 and the washing container insertion hole (13) therein; A first syringe container (20) having a built-in first syringe (30) for washing and separating adipose tissue, injecting a washing solution, and mixing a bio-gel (Bio-Gel) and a first syringe insert (11) therein; A second syringe container 21 having a plurality of bio-gel second syringes 40 filled with a biocompatible material for promoting induction of adipogenesis, and a second syringe container insert 12 having the same embedded therein; A connector container 23 having a connector 60 used for injecting a washing solution, mixing a washed adipose tissue with a biocompatible material, and a connector container insert hole 14 therein; and the kit box At the upper end of the needle container (24) with a needle for implantation (50) and a needle container insertion opening (15) therein; Protocol 25 designed to standardize fat transplantation and the protocol inlet 16 embedded therein; consists of a set kit (Kit) (10).

The present invention configured as described above minimizes the risk from infection in fat transplantation by treating and reconstructing the defective area by using the patient's own adipose tissue, which is implantable to the patient, and aseptic and sterilized instruments and standardized lipograft. By constructing the protocol of the kit into a set kit, it is possible not only to stably perform adipose tissue transplantation but also to induce adipose tissue regeneration along with the problem of existing adipose tissue transplantation. Solving at the same time, this allows the user to plant a good image, significantly improving the quality and reliability of the product.

Kits, syringes, flushes, connectors, protocols, adipose tissue, repair.

Description

Adipose tissue restoration kit {ADIPOSE TISSUE REGENERATION KIT}

1 is a kit showing a state in which a plurality of various containers of the present invention is not built

        Perspective view.

Figure 2 illustrates a state in which a plurality of various containers applied to the present invention is built into the kit

        Perspective view.

Figure 3 is a cross-sectional view of the first injector applied to the present invention.

Figure 4 is a cross-sectional view of the second injector applied to the present invention.

5 is an exploded perspective view of a needle container and a needle applied to the present invention.

Figure 6 is an exploded perspective view of a connector container and a connector applied to the present invention.

7 is a connector coupled between the first syringe and the second syringe applied to the present invention

        Schematic diagram of the state.

<Explanation of symbols for the main parts of the drawings>

10: kit 20: first syringe container

21: second syringe container 22: flushing container

23: connector container 24: needle container

25: PROTOCOL 30: First syringe

40: second syringe 50: needle

60: connector 62: protrusion

The present invention relates to a kit for repairing adipose tissue, and more particularly, to minimize the risk from infection, and to minimize the risk from infection, in the transplantation of adipose tissue that treats and reconstructs a defective site using the patient's own adipose tissue, which is implantable to the patient. Sterilized instruments and standardized lipoprotocol protocols in one set of kits not only make adipose tissue transplantation stable but also induce adipose tissue regeneration This solves the problem of the existing fat tissue transplantation at the same time, which allows the user to plant a good image, which can greatly improve the quality and reliability of the product.

According to Melvin A. Shiffman's "Autologous fat transplantation" book, the oldest fat transplant was Neuber's paper, published in 1893. He took almond-sized fat from a tuberculosis bone infection to fill part of his face. And good results.

Since then, many people have tried fat injection in various situations. The results were disappointing, but in 1895 Czerny tried to inject fat to fill the breast defect leading to benign tumor extraction, and Verderame introduced fat injection in ophthalmic surgery in 1909, but most of it was absorbed and more than needed. It was recommended to inject a large amount.

Since then, various attempts to inject fat have continued for about 100 years, but many doctors have been disappointed and abandoned because the results vary so much depending on the doctors that announce the results, and the technique of fat injection has been threatened to die. Later, as many methods for reducing absorption have been developed, the method is in the spotlight again.

Many papers on fat injection were initially disappointing, with absorption rates of over 50% within a year. Doctors who performed fat transplants pointed out many factors that interfere with engraftment and increase absorption of transplanted fat. The engraftment rate is different depending on which part of the body the fat is collected, the difference in the engraftment rate according to the method of collecting the fat is mentioned, and there have been experiments on how much pressure is appropriate for inhalation. Depending on the method of injection, the amount of injection, or the amount of injection, the injection rate is increased by adding betamethasone, insulin, or dextran at the time of injection. In order to increase the engraftment rate of fat infusion, proposals have been made for various methods of reducing absorption and increasing engraftment.

In 1928 Hilse histologically confirmed the formation of adipose tissue in free fat grafts and called the fat filled histocytes called lipoblasts. In 1947, Green used fat to treat secondary bone defects caused by osteomyelitis. He thought the transplanted fat would become connective tissue and eventually become bone-filled. In 1956, Peer transplanted fat into 20 pieces of walnut-sized chunks, and after testing fats that had been transplanted several times over a year, they found that the chunks had higher engraftment rates than the finely divided ones. In contrast, Coleman argued in his article in 1995 that it would be better to inject several portions at a time into a multi-layer because there are more opportunities for oxygen and nutrients.

As mechanical liposuction using cannula was made possible by A. Fisher and G. Fisher in 1974, efforts to use it to inject inhaled fat have continued since Bircoll was implemented in 1982.

Since then, the American Society of Plastic and Reconstruction Surgery (ASPRS) Ad-Hoc Committee on New Procedures suggested that autologous fat injection should have historical and scientific basis for autologous fat transplantation in 1987, and it is still an experimental procedure. It is so diverse that controlled clinical studies are needed to draw conclusions about the usefulness of fat infusion.

The conventional autologous fat graft as described above has been successful in hospitals under the guidance of doctors, and can be said to be a soft tissue defect transplantation that is being developed continuously. However, there are no examples of easy manipulation of fat transplantation and the provision of sterile and sterile instruments anywhere in the world, as well as kitted into a quantified concept under standardized protocols. I can't find a case. In other words, current adipose grafts have made much progress compared to the previous adipose grafts, but there is no suggestion of a standardized protocol or hygienic adipose preparation. In addition, various problems such as ulcers and complications of grafts after transplantation, inconvenience of having to retreat several times due to reduced volume after surgery, fibrosis of other tissues, and cell necrosis due to physical damage are still resolved. It remains a homework that must be done.

The present invention has been made to solve all the problems of the prior art as described above, one set of easy operation method and the provision of sterile and sterilized apparatus in fat transplantation can be found anywhere in the world The first purpose is to secure the technology that has been upgraded in local transplantation by providing kit to the domestic medical market as well as domestically. In the use of adipose tissue repair kits, a standardized protocol is provided for simple and easy operation, and the third purpose is to generate sterile and sterile treatments of all components in the kit. Minimize the problem from the risk of possible infection, and the fourth objective is to use a new concept of biocompatible material called Bio-Gel. Its purpose is to induce the regeneration of adipose tissue, and the fifth purpose is to supply the nutrients of fat using Bio-Gel to solve the problems such as transplantation tissue decay, complications, and volume reduction. The sixth objective is to provide a repair kit for adipose tissue that enables users to plant a good image, thereby significantly improving the quality and reliability of the product.

In order to achieve the above object, the present invention has a locking device at the bottom of a kit box, which is a sterile and sterilized adipose tissue washing solution filled with a washing container and a washing container insertion hole containing the same; A first syringe container containing a first syringe for mixing and washing adipose tissue, injecting a washing solution, and mixing a bio-gel, and a first syringe insert containing the same; A second syringe container containing a plurality of bio-gel second syringes filled with a biocompatible material for promoting induction of adipogenesis, and a second syringe container insertion hole containing the same; A connector container with a connector used for injecting a washing solution, a mixture of washed adipose tissue and a biocompatible material, and a connector container inlet for embedding the same; and the upper end of the kit box includes an injection needle 50 A built-in needle container and a needle container insert therein; It provides a kit for adipose tissue repair, characterized in that the protocol designed for the standardization of adipose transplantation and the protocol insertion therein is composed of a set kit (set).

Hereinafter, described in detail with reference to the accompanying drawings a preferred embodiment of the present invention for achieving this purpose are as follows.

The adipose tissue repair kit applied to the present invention is configured as shown in FIGS. 1 to 7.

In the following description of the present invention, if it is determined that the detailed description of the related known function or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be omitted.

The following terms are terms set in consideration of functions in the present invention, which may vary depending on the intention or custom of the producer, and the definitions should be made based on the contents throughout the present specification.

First, all kits used in the present invention can rebuild 100 ml of adipose tissue per kit, all components are limited to single use, and the components of the kits are used in a sterile space. As a sterile product, it can be protected from the risk of infection during adipose tissue transplantation.

In addition, a bottle filled with an adipose tissue washing solution is required to remove impurities from the adipose tissue obtained from the donor and to increase the maximum yield of the mature adipocytes and the preadipocytes in the adipose tissue. In this case, the flushing solution is used as a "solid tissue semi-solid gel composition for repairing adipose tissue" provided in the patent application No. 2005-0090202 filed on September 28, 2005, the composition is a solution for washing adipose tissue Adipose tissue was washed with collagen, adipogenic solution, serum, and mixed with the medium.

In addition, by using the solution for washing the adipose tissue of the present invention by providing the impurities of the adipose tissue obtained from the donor can be improved the problem of adipose tissue necrosis that can occur after adipose tissue transplantation.

In addition, the adipose tissue washing solution is filled first with a washing solution injection syringe to wash the adipose tissue, and a connector is used to wash the adipose tissue contained in the adipose tissue washing and separation syringe. The flushing solution contained in the syringe for flushing solution can be injected simply as shown. In addition, a syringe connector is used for mixing the washed adipose tissue and the bio-gel, which is composed in the kit, and the mixing is performed with a bio-gel mixing syringe. )do.

In order to inject a mixture of adipose tissue and bio-gel into a lipograft site, an injection needle is required.

There is also provided a standardized protocol (protocol) contained in the kit (kit) to achieve the object of the present invention, the present invention describes the process of the protocol (protocol).

In the present invention, the use range of the kit may be used in various ways such as humans and animals, and the adipose tissue transplantation site may also be used for a wide range of soft tissue defects such as breast, wrinkles, soft tissue defects, and the like. Hereinafter, the configuration of the present invention will be described in more detail.

The present invention is to be provided with a flushing container 22 and a flushing container insertion hole (13) that is filled with a sterile and sterilized adipose tissue washing solution at the bottom of the kit box with a lock device, The washing solution is filled with 300 ml in a sterilized washing container by filtering the washing solution, and the sealing solution is again sealed to prevent the solution from flowing into the inlet of the washing container 22.

In addition, the first syringe container 20 in which a plurality of first syringes 30, each of which washes and separates adipose tissue, injects a washing solution, and mixes a bio-gel, and a first injection device incorporating the same, 11) is equipped with a large amount of adipose tissue obtained from the donor at a time, easy to wash at once, and to remove impurities, the cap (50) to prevent the inlet leak out of each syringe 6 each after sterilization Pack in a set.

In addition, a second syringe container 21 having a plurality of bio-gel second syringes 40 filled with a biocompatible material for promoting induction of fat production and a second syringe container insertion hole 12 having the same embedded therein To be equipped with, to promote the induction of fat production and supply 2.5ml of bio gels to supply fat nutrients sterilized by filling in a 3ml syringe and then packed four (set).

In addition, a connector container 23 having a connector 60 used for injecting a washing solution, mixing a washed adipose tissue with a biocompatible material, and a connector container inserting hole 14 containing the same are provided. 4-5 pieces are packed in a set by sterilizing a syringe connector which is injected by syringe or used for easy mixing of washed adipose tissue and biocompatible material.

The present invention is also provided with a plurality of needle container container 24 and a needle container insertion hole (15) having a built-in needle 50 for implantation on the top of the kit box, mixed fat tissue In order to transplant to the implantation site, the injection needle for each transplant site is sterilized, and 3-6 pieces are packed in one set.

At this time, the first and second injector 20, 21, the needle container 24 and the inside of the connector container 23 is preferably configured in a sterile and sterilized state.

In addition, a screw portion 40a is formed on one side inner circumferential surface of the second syringe 40 so that the protrusion 62 protruding from the outer circumferential surface of the connector 60 is screwed, and the connector 60 passes through a flushing solution. The hole 61 is formed in the center.

In addition, the top of the kit box is provided with a protocol (10) designed for standardization of fat transplantation and a protocol insertion opening (16) therein as a set kit (10).

Meanwhile, the present invention may be variously modified and may take various forms in applying the above components.

And it is to be understood that the present invention is not limited to the specific forms mentioned in the above description, but rather includes all modifications, equivalents, and substitutions within the spirit and scope of the invention as defined by the appended claims. It should be understood that.

Referring to the method of using the subject innovation design kit designed as described above are as follows.

First of all, the present invention solves the problems of engraftment and absorption, which is a problem of conventional adipose graft, and induces adipose tissue regeneration, but can be easily and conveniently processed according to a standardized protocol while being sterilized under aseptic conditions. By using the components, the mixing of the processing (processing) fat tissue and bio-Gel (Bio-Gel) to promote the production of fat to provide a problem solving and simplicity of lipotransplantation at the same time, prior to the following embodiment Each step is described as follows.

That is, the present invention is to divide the adipose tissue collected from the donor into a total of four syringes of 25ml each in the 50ml first syringe 30 for washing and separating adipose tissue, which has a capping of the syringe inlet in advance. .

Thereafter, the adipose tissue washing solution is pre-filled using a 50 ml first syringe 30 for injecting a washing solution prepared in a kit 10, and 20 ml of the washing solution for adipose tissue is a syringe connector 60. Using a syringe filled with 25ml of adipose tissue, and after the injection again to cap the entrance with a cap (cap) and then shake the front and back of the syringe 10-25 times by hand to wash the adipose tissue.

After centrifugation after washing with water, a centrifugation step of autologous adipose tissue, which makes the adipose tissue dense and dense, is centrifuged at 1,200-3,000 rpm for 5-10 minutes after washing in the centrifugation step of the autologous adipose tissue. Separation is preferred.

In other words, when centrifugation of 1,200rpm and 5 minutes or less, the separation of impurities and fat layer and the obtaining of adipose tissue are inferior. Therefore, centrifugation of 1,200rpm and 5 minutes or more should be performed. When the separation is distinct, but because the fat precursor cells in the adipose tissue may be destroyed, centrifugation for 5 to 10 minutes at 1,200 ~ 3,000 rpm is preferable.

After the centrifugation, impurities under the adipose tissue are subjected to an impurity removal step of removing impurities by opening a cap of a syringe inlet to obtain impurities.

In addition, the step of repeating the washing step, centrifugation step, impurity removal step is to be repeated, wherein the repeating step, it is preferable to repeat a total of 3 to 4 times.

That is, less than three times the blood and other impurities can not be removed cleanly, four or more times, the repeating step is preferably three to four times because there is a risk of tissue necrosis due to the repeated washing and centrifugation process.

In addition, the present invention is a syringe containing dense and dense adipose tissue completely free of impurities and a bio-gel prepared in a kit to the adipose syringe using a syringe connector 60. Push-in, after pushing the bio-gel (Bio-Gel), the bio-gel second syringe 40 is removed, using a 50ml first syringe 30 for mixing the bio-gel (Bio-Gel) Push and pull 10-20 times to mix well.When mixing adipose tissue and Bio-Gel, add one syringe filled with Bio-Gel per syringe containing 25ml of adipose tissue. The mixing of the autologous adipose tissue and the biogel (Bio-Gel) is to be performed, wherein the biogel (Bio-Gel) is preferably mixed with a syringe filled with 2.0 to 3.0ml.

In other words, if the biogel is 2.0 or less, the mixing ratio with the adipose tissue may be low, which may reduce the uniformity of fat regeneration. If the biogel is 3.0 or more, the fat may be regenerated, but the biological properties (elasticity) may be reduced. Too rigid, there is a problem of feeling different from the original physical properties and the possibility that the tissue is fiberized, should be less than 3.0.

Finally, the present invention can be performed more easily and conveniently through adipose tissue transplantation through the step of transplanting the autologous adipose tissue to be injected into the treatment site by selecting the injection needle (50) to the site to inject the mixed adipose tissue Will be.

<Example>

The entire process is processed according to a standardized protocol constructed in adipose tissue repair kits. First, liposuction of 100 ml of patient's abdominal fat is divided into 4 ml syringes for adipose tissue washing and separation. Put it. The syringe opening is capped when dividing the adipose tissue to prevent leakage of the adipose tissue during the operation of the syringe. After preparing to wash the adipose tissue, 50 ml of the adipose tissue washing solution contained in the kit is placed in a washing solution injection syringe. Carefully open the cap of the syringe opening containing the adipose tissue to be washed, take out and connect one syringe connector, which is sterilized, and connect the syringe through the connector each 20 ml from the syringe containing the washing solution. Transfer to each other and close the syringe opening with a cap.

Shake the syringe back and forth slowly for 10-25 times to allow the wash solution and fat tissue to mix well. After visual confirmation that the adipose tissue was washed well, centrifuge for 5-10 minutes at 1,200-3,000rpm in a syringe-only centrifuge. After centrifugation, impurities in the adipose tissue lower layer are removed by opening a cap. This washing process, centrifugation process, and impurities removal process are repeated three times to obtain dense and dense adipose tissue and mixed with bio-gel filled with biocompatible materials that induce the regeneration of adipose tissue and fat. do. Mixing with Bio-Gel connects the syringe connector to push the Bio-Gel into the flushed adipose syringe and mix the Bio-Gel in the kit After changing to a 50ml syringe, push and pull both syringes for 10-20 times to mix fat tissue and Bio-Gel well. When mixing washed adipose tissue and Bio-Gel, syringe connectors should be used one by one to minimize the risk of infection. At this time, the mixing volume with bio-gel (Bio-Gel) per 25ml of adipose tissue should be 2.5ml in principle. After mixing with Bio-Gel, four syringes with a total volume of 27.5 ml were prepared and reconstituted with 100 ml of adipose tissue. After mixing, the size of the injection needle is selected according to the site to be implanted, and the injection is divided directly into the site.

As described in detail above, the present invention provides a kit for easy manipulation and providing sterile and sterilized instruments in fat transplantation, which cannot be found anywhere else in the world. It aims to secure technology that has been upgraded one step further in local transplantation by providing it to domestic and global medical markets. Especially, it is simple and easy to operate in the use of autologous adipose tissue repair kits. A standardized protocol was developed to minimize the problems associated with the risk of infection during aseptic and sterile treatment of all components in the kit and a new bio-gel. By using the concept of biocompatible materials, the regeneration of adipose tissue was induced. In addition, by using bio-Gel, Bio-Gel provides a new way to solve problems such as graft tissue decay, complications, and volume reduction by supplying fat nutrients. It is a very useful design that can be planted to greatly improve the quality and reliability of the product.

Claims (3)

At the bottom of the kit box is a locking device, a washing container 22 filled with sterile and sterilized adipose tissue washing solution and a washing container inserting hole 13 containing the same; A first syringe container (20) having a built-in first syringe (30) for washing and separating adipose tissue, injecting a washing solution, and mixing a bio-gel (Bio-Gel) and a first syringe insert (11) therein; A second syringe container 21 having a plurality of bio-gel second syringes 40 filled with a biocompatible material for promoting induction of adipogenesis, and a second syringe container insert 12 having the same embedded therein; It is provided with a connector container 23 and a connector container insertion hole (14) having a built-in connector 60, which is used when the washing solution is injected, washed with adipose tissue and biocompatible materials. On the upper end of the kit box, a needle container (24) with a built-in injection needle (50) and a needle container insertion opening (15) therein; A protocol for designing the standardization of adipose transplantation (25) and the protocol inlet (16) to embed it; a kit for adipose tissue repair, characterized in that it consists of a set kit (10). According to claim 1, The first and second injector (20) (21), the needle container (24) and the connector container 23, the interior of the adipose tissue repair kit, characterized in that the sterile and sterilized state. According to claim 1, A screw portion 40a is formed on one side inner circumferential surface of the second syringe 40 so that the protrusion 62 protruding from the outer circumferential surface of the connector 60 is screwed, and the connector has a hole in the center for passing a washing solution or the like. Adipose tissue repair kit, characterized in that formed.

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