KR101279652B1 - Device for separating platelet rich plasma with a coagulation catalyst, method for separating and coagulating platelet rich plasma using the same - Google Patents

Abstract

PURPOSE: A separating apparatus for platelet rich plasma is provided to remarkably shorten the solidification rate of PRP and plasma and enhance fibrin extraction productivity by performing second centrifuge in an inserting state of the coagulation catalyst of glass material within the upper chamber of the PRP separating device. CONSTITUTION: A separating apparatus (20) for platelet rich plasma comprises a main body (21) which has inner space and divided the inner space into upper liquid chamber (22) and lower liquid chamber (23); an upper cover (25) which covers the upper liquid chamber; a lower cover (26) that rises according to the inner periphery of the lower liquid chamber which outer periphery is adhered; a liquid collecting unit (24) that is formed between the upper liquid chamber and the lower liquid chamber and has a fluid path (24a) which connects the upper liquid chamber and the lower liquid chamber in the center unit in a upper protruded structure; and coagulation catalyst body (27) which is used as catalyst to rapidly coagulate the PRP and the plasma with a centrifuge by being inserted inside the upper liquid chamber.

Description

DEVICE FOR SEPARATING PLATELET RICH PLASMA WITH A COAGULATION CATALYST, METHOD FOR SEPARATING AND COAGULATING PLATELET RICH PLASMA USING THE SAME}

The present invention relates to a platelet enricher (Platelet Rich Plasma) separation device, a method for separating and coagulating autologous platelet concentrate using the same, and in detail, autoplatelet concentrate separated from blood by centrifugation (hereinafter referred to as PRP) And a platelet concentrate separating device having a coagulation catalyst body capable of extracting fibrin by rapidly solidifying plasma and a method for separating and coagulating self platelet concentrate using the same.

Blood carries the oxygen taken in the lungs to the tissue cells, carries the carbon dioxide from the tissues to the lungs to release them, transports the nutrients absorbed from the digestive tract to organs or tissue cells, and decomposes unnecessary substances in the living body as tissue degradation products. And transports hormone secreted from the endocrine glands to the organs and tissues to distribute the body heat uniformly to keep the body temperature constant. In addition, bacteria and foreign substances entering the living body are destroyed and detoxified (Detoxification) of the blood.

Some of these blood are used as a major index to determine various diseases or health conditions, but platelets rich in growth factors in the blood are used for therapeutic purposes. Blood is composed of red blood cells, white blood cells, and platelets. Among these, platelets are mainly present in plasma, and plasma is divided into PRP and PPP (Platelet poor plasma). PRP has been used for therapeutic purposes since it is implanted in the pain area, especially in the knee restraint, ligaments, muscles, etc., thereby stimulating stem cells to help produce cells.

PRP is a small amount of about 1% of the collected blood, and its high viscosity makes it difficult to separate from red blood cells, and research is being actively conducted on a technique for extracting PRP except red blood cells. In general, PRP is extracted by using a centrifuge after injecting blood taken from the human body into the PRP separator. In the past, the test tube was mainly used as a PRP separator. However, since PRP extraction is cumbersome, recently, a separator having a liquid chamber divided into two has been developed and used.

An example of such a PRP separation device has been proposed in the Republic of Korea Patent No. 10-1128163 (Registration Date: 12/12/2012) by the inventor of the present application, which is shown in FIG.

1 is a perspective view illustrating a PRP separator according to the prior art.

1, the PRP separation apparatus 10 according to the prior art is provided with an inner space, the inner space is divided into the upper liquid chamber 12 and the lower liquid chamber 13 and the upper liquid chamber ( 12 is installed to seal the upper end of the lower cap 15, and the lower end of the lower liquid chamber 13, the outer peripheral surface is in close contact with the inner peripheral surface of the lower liquid chamber 13 by an external force applied upward The lower stopper 16 coupled to ascendably in a state, and the fluid passage which divides the upper liquid chamber 12 and the lower liquid chamber 13 and communicates the upper liquid chamber 12 and the lower liquid chamber 13 at the center thereof protrude upwards. It consists of including a liquid collecting part 14 provided in a structure.

Referring to the PRP separation method using the PRP separation device 10 as follows.

First, the blood collected from the human body is injected into the lower liquid chamber 13 using a die, and then the PRP separator 10 into which the blood is injected is centrifuged using a centrifuge. When centrifugation is completed, red blood cells, PRP, and plasma are sequentially separated from the bottom in three stages by the specific gravity difference. Thereafter, the lower stopper 16 is raised to introduce some of the plasma contained in the lower liquid chamber 13 into the upper liquid chamber 12 via the fluid passage 14a. Thereafter, the lower stopper 16 is continuously raised so that the plasma contained in the lower liquid chamber 13 flows into the upper liquid chamber 12 through the fluid passage 14a, and the PRP is present in the fluid passage 14a. By introducing the syringe into the fluid passage 14a through the injection port of the stopper 15, the PRP present in the fluid passage 14a can be extracted, and the PRP can be easily separated and extracted.

Meanwhile, recently, PRP and plasma are separated and solidified to extract fibrin by using the above-described PRP separator, and fibrin is used as a base for implants for dental treatment, and the demand is steadily increasing. There is a trend. In the future, the demand for orthopedic surgeries is expected to explode.

PRP and plasma naturally begin to coagulate when exposed to air, but it takes a long time to solidify, so shortening the coagulation time of PRP and plasma is important for productivity. Accordingly, a method of coagulating PRP and plasma using a centrifugal separator while the PRP and plasma remain in the upper liquid chamber of the PRP separation device in which PRP and plasma are separated, thereby coagulating time of PRP and plasma Has been shortened to some extent.

However, as in the prior art, the method of coagulating PRP and plasma by centrifugation of PRP and plasma may shorten the coagulation time of PRP and plasma to some extent, but the time until coagulation is about 20-30 minutes. Since there is still a problem that productivity is lowered due to the need, research and development on a technology that can shorten the solidification time is urgently needed.

KR 10-1026599 B1, Mar. 25, 25, 3-6 KR 10-2011-0009651 A, Jan. 28, 2011, pp. 3-7 KR 10-1128163 B1, Mar. 12, 2012, pp. 6-8. Fig. 1-Fig. 5

Accordingly, the present invention has been proposed to solve the problems of the prior art, and provides an autologous platelet concentrate separating apparatus having a coagulation catalyst body capable of rapidly coagulating PRP and plasma separated from blood to improve fibrin extraction productivity. Its purpose is to.

Another object of the present invention is to provide a method for separating and coagulating autologous platelet concentrate using the autologous platelet concentrate separation device equipped with the coagulation catalyst.

The present invention according to one aspect to achieve the above object is provided with an inner space and the inner space is divided into an upper liquid chamber and a lower liquid chamber, an upper stopper for sealing the upper liquid chamber, the outer peripheral surface of the lower liquid chamber A lower stopper that rises along the inner circumferential surface of the lower liquid chamber while being in close contact with the inner circumferential surface, and is formed between the upper liquid chamber and the lower liquid chamber, and a fluid passage for communicating the upper liquid chamber and the lower liquid chamber in the center protrudes upward. It consists of a collecting part formed of a structure and a glass tube, a coagulation catalyst body which is inserted into the upper liquid chamber and serves as a catalyst so that the PRP and the plasma coagulate rapidly upon coagulation with PRP using a centrifuge It provides a self-platelet concentrate separating apparatus comprising a.

Preferably, the apparatus further includes a release preventing member coupled to a lower outer circumferential surface of the main body, wherein the release preventing member includes a first fastener formed with a screw thread on an inner circumferential surface to be coupled to a thread formed on the lower outer circumferential surface of the main body, and the first fastening member. A second fastener communicating with the sphere and having a thread formed on the inner circumferential surface with a diameter smaller than the first fastener may be included.

Preferably, the apparatus further includes a lifting member inserted into and fastened to the second fastening member of the release preventing member, wherein the lifting member has a bolt structure and is partially screwed with the second fastening member of the release preventing member to lower the liquid chamber of the main body. The volume of the lower liquid chamber may be varied by pulling the lower stopper upwardly in contact with the inner circumferential surface of the lower liquid chamber after being introduced into the upper portion.

In addition, the present invention according to another aspect for achieving the above object in the method for separating and coagulation of autologous platelet concentrate and plasma using the autoplatelet concentrate separation device described above, (a) the coagulation catalyst is the autoplatelet Injecting blood into the lower liquid chamber of the autologous platelet concentrate separator in the state of being inserted into the upper liquid chamber of the concentrate separation apparatus, and (b) centrifuging the autoplatelet concentrate concentrate apparatus in which blood is injected into the lower liquid chamber. Separating red blood cells, PRP, and plasma in the lower liquid chamber by first centrifugation through a separator; and (c) separating the lower stopper along the inner circumferential surface of the lower liquid chamber by using the elevating member. Injecting plasma into the upper liquid chamber through the fluid passage of the liquid collecting part; and (d) in the state where the coagulation catalyst body is present in the upper liquid chamber. Self-platelet concentrate material comprising the step of coagulating the PRP and the plasma introduced into the upper liquid chamber by centrifuging the PRP and the autologous platelet concentrate separating apparatus introduced into the upper liquid chamber through a centrifuge Provide separation and coagulation methods.

As described above, according to the present invention, by performing the second centrifugation in a state in which the coagulation catalyst body of glass material is inserted into the upper liquid chamber of the PRP separator, the coagulation rate of PRP and plasma can be significantly reduced. This can improve fibrin extraction productivity.

1 is a perspective view showing a PRP separator according to the prior art.
Figure 2 is an assembled perspective view showing a PRP separator having a coagulation catalyst body according to an embodiment of the present invention.
3 is an exploded perspective view showing the PRP separator shown in FIG.
4 is a cross-sectional perspective view of the PRP separator shown in FIG.
5 is a cross-sectional view of the PRP separator shown in FIG.
Figure 6 is an assembled perspective view showing a PRP separator having a coagulation catalyst body according to another embodiment of the present invention.
7 is an exploded perspective view showing the PRP separator shown in FIG.
8 is a cross-sectional perspective view of the PRP separator shown in FIG.
9 is a cross-sectional view of the PRP separator shown in FIG.
10 is a flowchart illustrating a method for separating and coagulating PRP and plasma according to an embodiment of the present invention.

BRIEF DESCRIPTION OF THE DRAWINGS The advantages and features of the present invention, and how to accomplish them, will become apparent by reference to the embodiments described in detail below with reference to the accompanying drawings. However, the present invention is not limited to the embodiments described below, but may be embodied in various other forms.

The present embodiments are provided so that the disclosure of the present invention is thoroughly disclosed and that those skilled in the art will fully understand the scope of the present invention. And the present invention is only defined by the scope of the claims. Thus, in some embodiments, well known components, well known operations, and well-known techniques are not specifically described to avoid an undesirable interpretation of the present invention.

In addition, like reference numerals refer to like elements throughout the specification. Moreover, terms used herein (to be referred to) are intended to illustrate the embodiments and are not intended to limit the invention. In the present specification, the singular form includes plural forms unless otherwise specified in the specification. Also, components and acts referred to as " comprising (or comprising) " do not exclude the presence or addition of one or more other components and operations.

Unless defined otherwise, all terms (including technical and scientific terms) used herein may be used in a sense commonly understood by one of ordinary skill in the art to which this invention belongs. Also, commonly used predefined terms are not ideally or excessively interpreted unless they are defined.

Hereinafter, the technical features of the present invention will be described in detail with reference to the accompanying drawings.

FIG. 2 is an assembled perspective view illustrating the PRP separator having a coagulation catalyst body according to an embodiment of the present invention, FIG. 3 is an exploded perspective view illustrating the PRP separator shown in FIG. 2 is a cross-sectional perspective view of the PRP separator shown in FIG. 2, and FIG. 5 is a cross-sectional view of the PRP separator shown in FIG.

2 to 5, the PRP separation apparatus 20 according to an embodiment of the present invention is provided with an inner space and the inner body 21 is divided into the upper liquid chamber 22 and the lower liquid chamber 23 Include.

The main body 21 has a cylindrical structure, and has a structure in which an upper portion and a lower portion are opened. The main body 21 may be made of a transparent material or a translucent material through which the internal space is projected.

The lower liquid chamber 23 of the main body 21 may have a diameter smaller than that of the upper liquid chamber 22 so that the main body 21 may be centrifuged in a state in which the main body 21 is stably housed inside the centrifuge. Accordingly, the stepped portion is formed at the boundary between the upper liquid chamber 22 and the lower liquid chamber 23.

The stepped portion formed in the main body 21 at the boundary between the upper liquid chamber 22 and the lower liquid chamber 23 is caught by the inlet of the accommodation portion when it is accommodated in the accommodation portion of the centrifuge, thereby centrifuging the lower portion of the main body 21. The bottom of the main body 21 can be prevented from being damaged by hitting the bottom of the receiving portion of the centrifuge during centrifugation by separating from the bottom of the receiving portion of the separator.

Meanwhile, the main body 21 according to the present invention is formed to have a structure in which the upper liquid chamber 22 and the lower liquid chamber 23 have different diameters, as shown in FIGS. 2 to 5. 21) is by no means limited to the structure shown in FIGS. For example, the upper liquid chamber 22 and the lower liquid chamber 23 may be formed to have the same diameter as shown in FIG. 1.

In addition, the PRP separator 20 according to the embodiment of the present invention includes an upper cap 25 fastened to cover and seal the upper opening of the main body 21, that is, the upper liquid chamber 22. To this end, the upper outer peripheral surface of the main body 21, the thread (21a, Fig. 3) is fastened with the screw thread (25a, Fig. 5) formed on the inner peripheral surface of the upper stopper 25 to seal the opened portion of the upper liquid chamber 22 Is formed).

As shown in FIG. 3, the upper stopper 25 is provided with an injection port 25b for injecting blood collected from the human body into the fluid passage 24a in the center portion or for extracting the PRP separated in the main body 21. . The injection port 25b is sealed by the packing member 30 made of a soft material into which the needle of the syringe can be inserted, and is formed to face the fluid passage 24a.

The packing member 30 is installed to be fixed to the top of the upper stopper 25 by the fixing pin 31 to prevent separation from the upper stopper 25 when opening the injection hole 25b as shown in FIG. It is preferable. That is, the packing member 30 penetrates through the fixing groove 30a provided at one side of the packing member 30 and is fixed to the coupling groove 25c provided in the upper stopper 25 by one side of the fixing pin 31. As the additional upper stopper 25 is fixedly installed, a portion of the upper stopper 25b is fixed without being separated from the upper stopper 25 even when the injection port 25b is opened, thereby reducing the risk of loss.

In addition, the PRP separator 20 according to the embodiment of the present invention has a lower outer peripheral surface so that blood injected into the lower liquid chamber 23 does not leak into the lower opening of the main body 21 as shown in FIGS. 4 and 5. And a lower stopper 26 rising along the inner circumferential surface of the lower liquid chamber 23 while being in close contact with the inner circumferential surface of the liquid chamber 23.

The lower stopper 26 is inserted through the lower opening of the main body 21 to seal the lower liquid chamber 23 and at the same time a strong pressure is applied from the lower part to the upper part while the outer peripheral surface is in close contact with the inner peripheral surface of the lower liquid chamber 23. In the case of the lower liquid chamber 23 rises in the direction of the liquid collection part (24).

In addition, the PRP separator 20 according to an embodiment of the present invention is formed between the upper liquid chamber 22 and the lower liquid chamber 23 as shown in Figures 2 to 5 and the upper liquid chamber 22 and the lower portion in the center The liquid passage 24a for communicating the liquid chamber 23 includes a liquid collecting part 24 provided in a structure protruding upward.

The liquid collecting part 24 is formed in a curved structure having a curved shape that rises from the outer part to the central part as shown in FIGS. 4 and 5. The reason is to prevent the separated substances from mixing when the volume of the lower liquid chamber 23 is changed after centrifugation. This is because the tapered shape interferes with the change of the volume of the lower liquid chamber 23, thereby inhibiting the rise of the PRP.

The fluid passage 24a of the liquid collection part 24 is provided spaced apart from the inner surface (ceiling surface) of the upper stopper 25 by a predetermined distance. The bottom part communicates with the lower liquid chamber 23 and the upper part is the upper liquid chamber 22. ) Has a structure in communication with. The collection part 24 as a whole has a trumpet shape placed downward.

In addition, the PRP separation apparatus 20 according to an embodiment of the present invention is inserted into the upper liquid chamber 22 in a cylindrical tube structure as shown in Figure 3 PRP using a centrifugal separator and PRP and plasma during coagulation It includes a coagulation catalyst body 27 that serves as a catalyst to coagulate quickly.

The coagulation catalyst body 27 is made of a glass material, and the outer circumferential surface may be installed to be in contact with the inner circumferential surface of the upper liquid chamber 22, or may be installed in a state spaced apart from the inner circumferential surface of the upper liquid chamber 22 by a predetermined distance. Preferably, in order to prevent the coagulation catalyst body 27 from being independently rotated in the upper liquid chamber 22 and broken by a collision during centrifugation using a centrifuge, the outer circumferential surface contacts the inner circumferential surface of the upper liquid chamber 22. do.

On the other hand, the PRP separator 20 according to the present invention, as shown in Figure 3, the lifting member 32, 6 to Fig. 6 to raise the lower plug 26 in the upper direction on the lower surface of the lower plug 26 9 may further include a reinforcing member 28 (see FIGS. 3 and 5) fastened to support the lower plug 26 from an external force applied to the lower portion of the lower plug 26.

In addition, the PRP separator 20 according to the embodiment of the present invention is a lower portion of the main body 21 to prevent the lower plug 26 is separated from the main body 21 by a strong rotational force during centrifugation using a centrifuge It may further include a separation preventing member 29 is screwed on. This detachment preventing member 29 has a substantially cylindrical lid structure as shown in FIG.

Referring to FIG. 3, the separation preventing member 29 includes a first fastener 29a having a thread 29a-1 formed on an inner circumferential surface thereof to be fastened with a thread 21b formed on an outer circumferential surface of the main body 21, and a bolt structure. A second in communication with the first fastener (29a) and the screw thread (29b-1) formed on the inner circumferential surface in order to be engaged with the lifting member 32 (see Figs. 6 to 9) formed with the thread (32-2a) on the outer periphery of the furnace Fastener 29b. At this time, the second fastener 29b is formed to be smaller than the diameter of the first fastener 29a so as not to contact the first fastener 29a when the rising member 32 is screwed through.

6 is an assembled perspective view illustrating the PRP separator having a coagulation catalyst body according to another embodiment of the present invention, FIG. 7 is an exploded perspective view illustrating the PRP separator shown in FIG. 6, and FIG. 8. 6 is a cross-sectional perspective view of the PRP separator shown in FIG. 6, and FIG. 9 is a cross-sectional view of the PRP separator shown in FIG. 6.

6 to 9, the PRP separator 120 according to another embodiment of the present invention is different from each other except for the PRP separator 20 and the lifting member 32 shown in FIGS. 2 to 5. As described above, the same configuration will be replaced with the above description, and hereinafter, a configuration related to the lifting member 32 will be described in detail.

The lifting member 32 has a bolt structure as shown in FIG. 7, and in particular, an operation part 32-1 that can be operated by a user by hand, and one side part are integrally fastened with the operation part 32-1. The other end portion is inserted into the second fastener 29b of the release preventing member 29 and a male thread is formed on the outer circumferential surface thereof so that the fastening portion 32-2 fastened with the second fastening portion 29b of the release preventing member 29. ).

The lifting member 32 is formed so that a part of the fastening part 32-2 is engaged with the thread 29b-1 formed on the inner circumferential surface of the second fastener 29b (see FIG. 3) of the separation preventing member 29. The thread 32-2a is formed in the outer peripheral surface of the site | part inserted into the 2 fastener 29b. Accordingly, the elevating member 32 is inserted into the second fastener 29b of the separation preventing member 29 in a screw coupling manner and coupled to the second fastener 29b of the separation preventing member 29 to be elevated. The volume of the lower liquid chamber 23 is varied by raising the lower plug 26 provided in the lower liquid chamber 23 to the upper portion in the lifting operation.

Hereinafter, the PRP and plasma coagulation method using the PRP separation device 120 according to another embodiment of the present invention will be described.

10 is a flowchart illustrating a method for separating and coagulating PRP and plasma according to an embodiment of the present invention.

First, referring to Figures 6 to 10, first prepare a PRP separator 120 (ST1).

The preparation step of the PRP separation device 120, the upper plug 25 of the main body 21 in the state in which the coagulation catalyst body 27 is inserted into the upper inside of the main body 21, that is, the upper liquid chamber 22 first; The upper part of the upper liquid chamber 22 is closed by being fastened to the upper part, and the lower plug 26 is inserted into the lower part of the main body 21, that is, the lower liquid chamber 23 by the interference fitting method. Seal the lower part. In addition, the release preventing member 29 may be fastened to the outer outer surface of the main body 21.

Thereafter, the PRP separator 120 is sterilized in a state in which the coagulation catalyst body 27 is inserted into the upper liquid chamber 22 (ST2).

Thereafter, the blood collected from the human body through the injection port 25b of the upper stopper 25 is injected into the inside of the main body 21, that is, the lower liquid chamber 23 through the fluid passage 24a of the collection part 24 ( ST3).

Thereafter, the blood is injected into the lower liquid chamber 23, the PRP separator 120 is accommodated in a centrifuge, and the blood is first centrifuged to separate red blood cells, PRP, and plasma in the lower liquid chamber 23 (ST4). .

Thereafter, the PRP separator 120 having the first centrifugal separation is withdrawn from the centrifuge, and then the lifting member 32 is introduced into the lower liquid chamber 23 through the second fastener 29b of the separation preventing member 29. Insert it. Then, the rising member 32 is rotated in a clockwise direction to raise the lower stopper 26 along the inner circumferential surface of the lower liquid chamber 23, excluding red blood cells, PRP, and red blood cells in the plasma. PRP and plasma are introduced into the upper liquid chamber 22 through the fluid passage 24a of the collecting part 24 to separate the PRP and plasma from the red blood cells (ST5).

Thereafter, the PRP and plasma are introduced into the upper liquid chamber 22 and separated from the erythrocytes, and the lifting member 32 is separated from the separation preventing member 29 and then the PRP separation apparatus 120 according to the embodiment of the present invention. The second centrifugation is carried out by receiving in a centrifuge (ST6). Then, the second centrifugation is performed for 3 to 5 minutes to coagulate PRP and plasma introduced into the upper liquid chamber 22 to extract fibrin.

The secondary centrifugation performed in the step ST6 is performed in a state in which the coagulation catalyst body 27 made of glass is inserted into the upper liquid chamber 22, so that the secondary centrifugation in the state in which the coagulation catalyst body 27 is not present. It is possible to shorten the coagulation rate of PRP and plasma than to perform.

In order to confirm the coagulation rate of PRP and plasma through the second centrifugation, the second centrifugation was performed while the coagulation catalyst body 27 was inserted into the upper liquid chamber 22, until the PRP and the plasma coagulated. It took 3 to 5 minutes. On the contrary, when the second centrifugation was performed without the coagulation catalyst body according to the present invention inserted into the upper liquid chamber 22, it was experimentally confirmed that it took about 20-30 minutes until PRP and plasma coagulated. Could.

As described above, in the present invention, by performing the second centrifugation while the coagulation catalyst body 27 made of glass is inserted into the upper liquid chamber 22, the coagulation rate of PRP and plasma can be significantly reduced. This can improve fibrin extraction productivity.

As described above, the technical spirit of the present invention has been described in detail in the preferred embodiments, but the above-described preferred embodiments are for the purpose of description and not of limitation. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

20, 120: auto platelet concentrate separation device (PRP separation device)
21:
22: upper liquid chamber
23: lower liquid chamber
24: collection part
24a: fluid passage
25: upper stopper
26: lower stopper
27: coagulation catalyst
28: reinforcing member
29: separation prevention member
30: packing member
31: fixed pin
32: rising member

Claims (4)

An inner space is provided and the inner space is divided into an upper liquid chamber and a lower liquid chamber;
An upper cap sealing the upper liquid chamber;
A lower stopper which rises along the inner circumferential surface of the lower liquid chamber while the outer circumferential surface is in close contact with the inner circumferential surface of the lower liquid chamber;
A liquid collecting part formed between the upper liquid chamber and the lower liquid chamber, and having a structure in which a fluid passage protruding upward from the upper liquid chamber to communicate with the lower liquid chamber in a central portion thereof; And
It is made of a glass tube, which is inserted into the inside of the upper liquid chamber and the coagulation catalyst body acts as a catalyst so that the PRP and the plasma coagulate rapidly when PRP and plasma coagulation using a centrifuge
Self-platelet concentrate separating apparatus comprising a.
The method of claim 1,
And a detachment preventing member fastened to the lower outer circumferential surface of the main body, wherein the detachment preventing member is in communication with the first fastener formed with a screw thread on the inner circumferential surface to be fastened with the thread formed on the lower outer circumferential surface of the main body. An apparatus for separating autologous platelet concentrate comprising a second fastener formed with a thread on an inner circumferential surface with a diameter smaller than that of the first fastener.
3. The method of claim 2,
And a rising member inserted into and fastened to the second fastener of the release preventing member, wherein the lifting member is bolted to a part of the second fastener of the release preventing member to be drawn into the lower liquid chamber of the main body. And a platelet concentrate separating apparatus for varying the volume of the lower liquid chamber by raising the lower plug closely attached to the inner circumferential surface of the lower liquid chamber.
In the method for separating and coagulating autologous platelet concentrate and plasma using the autologous platelet concentrate separator of claim 3,
(a) injecting blood into the lower liquid chamber of the autoplatelet concentrate separator while the coagulation catalyst body is inserted into the upper liquid chamber of the autoplatelet concentrate separator;
(b) separating the erythrocytes, PRP, and plasma in the lower liquid chamber by first centrifuging the autologous platelet concentrate separator in which blood is injected into the lower liquid chamber through a centrifugal separator;
(c) using the lifting member to raise the lower stopper along the inner circumferential surface of the lower liquid chamber to introduce separated PRP and plasma into the upper liquid chamber through a fluid passage of the liquid collecting part; And
(d) centrifuging the autologous platelet concentrator separating the PRP and the plasma into the upper liquid chamber in a state where the coagulation catalyst is present in the upper liquid chamber through a centrifugal separator and entering the upper liquid chamber; Coagulating PRP and the plasma
Self-platelet concentrate separation and coagulation method comprising a.

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